Rational Design and Optimisation of CRISPR-Cas9 Delivery Systems for Targeted Genomic Transformation Kalpana Pandya, Likshit S. Jaisinghani, Avanish Tripathi, Dileep Kumar, Sunny Kumar Saraf, Jagannath Sahoo, Devendra Kumar Journal of Gene Medicine, 2026 The CRISPR‐Cas9 genome‐editing technique offers a promising therapeutic strategy for genetic disorders, including neurodegenerative diseases like Alzheimer's disease (AD), characterised by inherited susceptibility and progressive cognitive decline, as well as other hallmarks such as amyloid beta (Aβ 1–42 ) plaques and neurofibril tangles (NFTs). However, the blood–brain barrier (BBB) poses a significant challenge to the effectiveness of gene editing components in the affected brain region and impedes clinical translation. This comprehensive review compares various CRISPR‐Cas9 delivery vectors, viral, nonviral and physical, with a focus on their efficacy in neurological diseases such as AD. Viral vectors viz., adeno‐associated viruses (AAVs) and lentiviruses (LVs) demonstrate high transduction efficiency and BBB permeability. AAVs are preferred for their low immunogenicity, minimal toxicity, high neuronal tropism and episomal persistence, enabling sustained expression without insertional mutagenesis. LVs offer larger genetic payloads but raise concerns about genomic integration and potential oncogenesis, though integration‐defective variants mitigate these risks. Nonviral vectors, including peptide and polymer‐based nanoparticles, lipid nanoparticles (LNPs) and Inorganic carriers such as gold and silver nanoparticles, are less immunogenic and easier to handle but require further optimisation for in vivo BBB crossing and endosomal escape. Physical methods such as electroporation and microinjection are suitable for in vitro/ex vivo use, while novel CNS‐targeted strategies, such as RVG‐tagged particles, TfR‐directed LNPs and engineered AAV variants, enhance brain penetration via receptor‐mediated transcytosis. These preclinical studies show that these technologies can successfully edit genes and provide therapeutic benefits, including amyloid reduction and cognitive improvement in AD models. Yet off‐target effects, immune responses and regulatory hurdles persist. Overall, continuous innovation in delivery vector design and safety profile‐targeting strategies is crucial for advancing CRISPR‐Cas9 towards clinical therapies for AD‐based therapies and related neurological disorders.
QbD-guided development and optimization of a rapid-disintegrating sublingual film of olanzapine Devendra Patil, Bappaditya Chatterjee, Jagannath Sahoo, Rohan Pai Therapeutic Delivery, 2026 AIM: Olanzapine is an atypical antipsychotic used to treat chronic schizophrenia and bipolar I disorder, including mixed/manic episodes. It is a BCS class II medication, and thus there is a potential for sublingual administration allowing rapid absorption. This may potentially result in a faster onset of action compared to oral tablets. The aim of this work was to formulate a fast-dissolving sublingual film of olanzapine with rapid disintegration. MATERIALS AND METHODS: drug release of films. RESULTS: dissolution studies estimated the release to be 94.79% in 10 min. CONCLUSION: assessment of the film are warranted to prove enhanced permeation indicating quicker absorption sublingually, leading to an increase in bioavailability.
Case Studies: Success Stories in Green Grafting Yashmi Vasani, Koteswara Rao GSN, Jagannath Sahoo, Sakshi Parab, Viraj Amin Green Grafting Innovations in Polymer Functionalization for Sustainable Solutions in Pharmaceutical and Healthcare Industry Part 2, 2026 The current developments in green nanotechnology, which have traditionally been limited to gold nanoparticles (AuNPs) used for in vitro biocompatibility testing, have addressed many issues. Researchers are interested in knowing several green nanotechnological protocols and how they are being employed to functionalize gold nanoparticles. Different characterization tools are required to determine the size and physicochemical properties of gold nanoparticles. An overview of the various applications of gold nanoparticles in diagnostic imaging techniques, such as X-ray contrast agents, SPECT imaging, PET imaging, OCT, fluorescence imaging, MRI, SERS, and ultrasound imaging, is of particular interest to researchers. Moreover, this chapter will also focus on drug delivery systems, photothermal therapy, cancer therapy, and molecular imaging, as well as the therapeutic potential of phytochemicals as reducing agents for gold nanoparticles. The systemic toxicity of gold nanoparticles and the strategies to address this toxicity are also important for their effective use in biomedical science. In conclusion, our chapter highlights the numerous potential roles of gold nanoparticles in nanomedicine, while drawing attention to some of the shortcomings associated with their applications. In turn, organic polymers find applications in adhesion, biomaterials, protective coatings, tribology (including friction, lubrication, and wear), advanced composites, and microelectronics, as well as in the rapidly advancing field of thin-film technology. These polymers are important in the plastic industry, but surface modification techniques are used to convert them into useful products. Polymers are suitable for implants and tissue engineering scaffolds because plasma treatment increases cell adhesion, growth, and proliferation in the biomedical field.
Fabrication and characterization of buccal films of propranolol hydrochloride: an in-vitro and ex-vivo analysis Prakash Kumar, Jagannath Sahoo, Bhavna Kumar, Abhijeet Ojha, Abdul Ahad, Islauddin Khan Journal of Dispersion Science and Technology, 2026 The aim of current examination was to formulate propranolol hydrochloride (PHCL) loaded buccal films by combining polyvinyl alcohol with guar gum using solvent casting process. The formulated films were assessed for a variety of measures including physical evaluation, in vitro drug permeation, in vitro drug release and ex vivo permeation studies. The prepared PHCL loaded films demonstrated homogeneous surface, and the weight uniformity of prepared films was noted in the range of 160 ± 0.29 mg to 169 ± 0.32 mg. The thickness and swelling index of fabricated films were found in the range of 0.132 ± 0.013 mm to 0.264 ± 0.011 mm and 38.73 ± 1.02% to 46.99 ± 1.92%, respectively. The pH of all developed films was near to neutral (pH 6.41 ± 0.71 to pH 6.59 ± 0.91). The folding endurance and drug content of all prepared films were within the range of 74.33 ± 4.04 to 150.00 ± 2.00 and 91.38 ± 0.71% to 97.34 ± 0.13%, respectively. The formulation F2 exhibited maximum in vitro drug permeation (85.11 ± 4.26%) across egg shell membrane, in vitro drug release of 96.66 ± 6.01% and ex vivo drug permeation of 87.36 ± 3.80% across buccal mucosa. The findings of ex vivo permeation through buccal mucosa exhibited that the drug permeation rate was sustained for 360 minutes. A buccal film containing PHCL shows tremendous potential for systemic administration. In addition to circumventing hepatic first-pass metabolism, this method has the advantage of minimizing side effects. To ensure the efficacy of the prepared formulation, further studies in an animal model are recommended.
Structural Characterization, In Vitro Antidiabetic Activity, and Molecular Docking Studies of Isolated Compounds From Allamanda cathartica Ritu Tomar, Shashank Shekher Mishra, Vivek Sahu, Jagannath Sahoo, Mandeep K. Arora, Santosh Kumar Rath Chemistry and Biodiversity, 2025 Allamanda cathartica is a perennial shrub, traditionally used for the treatment of several ailments, including jaundice, malaria, and enlargement of the spleen. Besides ornamental values, the plant also possesses a wide variety of medicinal properties due to the presence of bioactive secondary metabolites of different chemical classes. The present work describes the phytochemical investigation of the leaf part of A. cathartica, which resulted in the isolation of five secondary metabolites (ACL 1–5) of different classes, including β‐sitosterol (ACL‐1), β‐sitosterol glucoside (ACL‐2), ursolic acid (ACL‐3), plumieride (ACL‐4), and pinitol (ACL‐5). The compound ACL‐5 was reported for the first time from the species. All the compounds were characterized by 1H NMR, 13C NMR, 2D NMR, and HRMS spectral analysis. Further to the plant extract, all the fractions and five isolated compounds were screened for in vitro α‐amylase and α‐glucosidase activity. The isolated compounds exhibited notable inhibitory activity against carbohydrate‐hydrolyzing enzymes. For α‐amylase inhibition, IC50 values ranged from 522.95 to 662.67 µg/mL, with plumieride showing the highest potency at a concentration of 522.95 µg/mL and pinitol acetate the lowest at 662.67 µg/mL. Similarly, for α‐glucosidase inhibition, IC50 values ranged from 576.82 to 691.12 µg/mL, where plumieride again demonstrated the strongest activity at 576.82 µg/mL and pinitol acetate the lowest at 662.67 µg/mL. In support, all isolated compounds were taken further for molecular modelling studies to observe the rationale of our in vitro studies. Therefore, this work concludes that the isolated compounds ACL‐4 and ACL‐5 have demonstrated strong antidiabetic potential, with ACL‐4 having a somewhat more powerful antidiabetic effect than ACL‐5, according to both in vitro and docking experiments.
Luliconazole bilosomal gel for treating fungal infection: development, optimization and antifungal activity Kartik Aralelimath, Jagannath Sahoo, Sarika Wairkar Therapeutic Delivery, 2025 AIMS Luliconazole is a class of imidazole that exhibits high antifungal activity. Luliconazole has drawbacks, such as low aqueous solubility and poor skin penetration. METHODS To overcome these limitations, luliconazole-loaded bilosomes (LZBSs) were developed via ethanol injection, using soy lecithin, cholesterol, Span 60, and bile salt. A 23 factorial design was employed to optimize the formulation. RESULTS The optimized batch of LZBS resulted in a vesicle size of 177.3 ± 0.00 nm with an entrapment efficacy of 90.0 ± 2.5 % and zeta potential of -54.8 ± 3.15 mV. TEM analysis confirmed the spherical shape of bilosome vesicles and ATR-FTIR results supported the formation of bilosomes without any interaction. LZBS was loaded into Carbopol gel (LZBS gel) and evaluated for in vitro and ex vivo drug release study; results showed extended release of 90.53 ± 7.89 % and 84.97 ± 5.58 %, respectively, up to 24 h. Antifungal study for LZBS gel demonstrated superior activity against Candida albicans as compared to marketed and pure drug. CONCLUSION Thus, luliconazole-loaded bilosome gel proved to be effective against fungal infections.
Population Modelling Devendra Patil, Jagannath Sahoo, Rohan Pai, S. N. Koteswara Rao G. Applications of Computational Tools in Drug Design and Development, 2025
A Comprehensive Review of Experimental Animal Models of Hepatopathy Ramesh Kumar Gupta, Sachin Chaudhary, Abdel-Nasser El-Shorbagi, Udaivir Singh Sara, Prashant Shukla, Sudhansu Ranjan Swain, Jagannath Sahoo, P. N. Murthy P. N. Murthy Biomedical and Pharmacology Journal, 2022
Development of fast dissolving tablets levocetirizine dihydrochloride by sublimation method International Journal of Pharmacy and Technology, 2013
Enhancement of dissolution rate of glimepiride using solid dispersions with polyvinylpyrrolidone K 90 Indian Journal of Pharmaceutical Education and Research, 2010
Formulation design and optimization of mouth dissolving tablets of levocetirizine hydrochloride using sublimation technique Indian Journal of Pharmaceutical Education and Research, 2009
Fabrication and characterization of buccal films of propranolol hydrochloride: an in-vitro and ex-vivo analysis P Kumar, J Sahoo, B Kumar, A Ojha, A Ahad, I Khan Journal of Dispersion Science and Technology 47 (6), 951-958 , 2026 2026
Case Studies: Success Stories in Green Grafting Y Vasani, KR GSN, J Sahoo, S Parab, V Amin Green Grafting: Innovations in Polymer Functionalization for Sustainable … , 2026 2026
Versatile potential of ultrasound mediated microbubble for biomedical applications: Current trends and emerging strategies MBP Trisha H. Sarkar, Utkarsh U. Bhamare, Jagannath Sahoo Journal of Drug Delivery Science and Technology 120, 1-15 , 2026 2026
Transcending Cellular Barriers by Leveraging Nano-Bio Interactions in Nanomaterial-Driven Disease Therapy D Tripathi, V Shukla, RK Tiwari, J Sahoo Current Topics in Medicinal Chemistry , 2026 2026
Structural Characterization, In Vitro Antidiabetic Activity, and Molecular Docking Studies of Isolated Compounds From Allamanda cathartica R Tomar, SS Mishra, V Sahu, J Sahoo, MK Arora, SK Rath Chemistry & Biodiversity 22 (12), e01183 , 2025 2025 Citations: 1
Luliconazole bilosomal gel for treating fungal infection: development, optimization and antifungal activity. SW Aralelimath K., Jagannath Sahoo J Therapeutic Delivery 16 (8), 733-743 , 2025 2025 Citations: 2
Design and evaluation of casein nanoparticles for co-delivery of curcumin and fluorouracil acetic acid in cancer therapy A Chaudhary, A Behera, J Sahoo Medical Oncology 42 (8), 335 , 2025 2025 Citations: 1
Design and Evaluation of a Data-Driven Youth Well-Being Dashboard: A Pre-Post Mixed-Methods Study in Indian Colleges B Malhotra, S Bhargava, A Joshi, J Sahoo, M Singhal, RL Musunuri National Journal of Community Medicine 16 (07), 655-667 , 2025 2025
Enhancing Ketoprofen Solubility: A Strategic Approach Using Solid Dispersion and Response Surface Methodology D Tripathi, DK Sharma, J Sahoo Current Radiopharmaceuticals 18 (2), 100-119 , 2025 2025 Citations: 3
Exploring the Feasibility of Accelerometer-Based Mobile Health Apps to Enhance Physical Activity in Western Rajasthan V Arora, SS Mishra, A Joshi, J Sahoo, N Joshi, V Joshi, K Bajaj Indian Journal of Science and Technology 18 (20), 1547-1553 , 2025 2025
Molecularly imprinted polymer-based biosensor for detection of salivary glucose in diabetes PS Arjvee Vaidya, Jagannath Sahoo International Journal of Pharmaceutics 67, 1-11 , 2025 2025
Dermal drug delivery via bilosomes: a synergistic integration for better therapeutic outcomes SW Kartik Aralemiath, Jagannath sahoo Journal of Microencapsulation 41 (8), 818-831 , 2024 2024 Citations: 5
An experimental approach to development and evaluation of hepatoprotective polyherbal formulation JSKM Ramesh Kumar Gupta, Sudhansu Ranjan Swain Indian Journal of Experimental Biology 62, 1-12 , 2024 2024
Aiding Clinical Decision-Making at the Individual and Community Level Using Mobile Sensor Data: Study Protocol for an Experimental Design SSMAJMSJS Vikas Arora Cureus , 2024 2024 Citations: 1
Computational and in-vitro Investigation of Phytochemicals from Allamanda cathartica as a Potential Candidate for the Treatment of Type 2 Diabetes mellitus R Tomar, SS Mishra, J Sahoo, SK Rath Journal of Computational Biophysics and Chemistry 23 (07), 901-923 , 2024 2024 Citations: 9
In Silico and In Vitro Investigation of Phytoconstituents from Flacourtia Jangomas as a Potential Candidate for the Treatment of Type 2 Diabetes Mellitus R Tomar, SS Mishra, J Sahoo, SK Rath ChemistrySelect 9 (36), e202400542 , 2024 2024 Citations: 3
Quality by design approach for the formulation of bilayer tablets of domperidone and itopride in gastro-esophageal reflux disease R Prajapati, B Kumar, J Sahoo, S Shakya, DK Lal Journal of Applied Pharmaceutical Science 14 (8), 169-181 , 2024 2024 Citations: 2
Assessment of Anti-Alzheimer Characterization of Green Synthesized Zinc Nanoparticles Containing Extract of Cordyceps militaris K Saini, S Shilpi, S Arya, J Sahoo Indian Journal of Pharmaceu-tical Education and Research 58, s925-s933 , 2024 2024 Citations: 2
Navigating the solution to drug formulation problems at research and development stages by amorphous solid dispersion technology D Tripathi, MP BH, J Sahoo, J Kumari Recent Advances in Drug Delivery and Formulation: Formerly Recent Patents on … , 2024 2024 Citations: 14
Computational investigation of phytochemicals from Allamanda cathartica as a potent agonist of Peroxisome proliferator-activated receptor-gamma (PPARγ) for the treatment of … R Tomar, S Mishra, J Sahoo, SK Rath 2024
MOST CITED SCHOLAR PUBLICATIONS
Enhancement of dissolution rate of gliclazide using solid dispersions with polyethylene glycol 6000 S Biswal, J Sahoo, PN Murthy, RP Giradkar, JG Avari Aaps Pharmscitech 9 (2), 563-570 , 2008 2008 Citations: 203
Formulation and process optimization of naproxen nanosuspensions stabilized by hydroxy propyl methyl cellulose B Mishra, J Sahoo, PK Dixit Carbohydrate polymers 127, 300-308 , 2015 2015 Citations: 82
Comparative study of propranolol hydrochloride release from matrix tablets with Kollidon® SR or hydroxy propyl methyl cellulose J Sahoo, PN Murthy, S Biswal, SK Sahoo, AK Mahapatra Aaps Pharmscitech 9 (2), 577-582 , 2008 2008 Citations: 82
Formulation of Sustained Release dosage form of Verapamil Hydrochloride by Solid Dispersion Technique using Eudragit RLPO or Kollidon SR PNM J Sahoo, S Biswal AAPS PharmSciTech. 10 (1), 27-33 , 2009 2009 Citations: 81
Physicochemical properties of solid dispersions of gliclazide in polyvinylpyrrolidone K90 S Biswal, J Sahoo, PN Murthy Aaps Pharmscitech 10 (2), 329-334 , 2009 2009 Citations: 69
Enhanced bioavailability of cinnarizine nanosuspensions by particle size engineering: Optimization and physicochemical investigations B Mishra, J Sahoo, PK Dixit Materials Science and Engineering: C 63, 62-69 , 2016 2016 Citations: 65
Characterisation of gliclazide-PEG 8000 solid dispersions S Biswal, J Sahoo, PN Murthy Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City … , 2010 2010 Citations: 59
Solid Dispersions: a technology for improving Bioavailability KA Sharma KS, Sahoo J Journal of Analytical and Pharmaceutical Research , 2019 2019 Citations: 49
A comparative study of controlled release matrix tablets of Diclofenac Sodium, Ciprofloxacin Hydrochloride, and Theophylline . SJ Saha RN, Sanjeev C Drug Delivery 8, 149-154 , 2001 2001 Citations: 29
Therapeutic implications of dietary polyphenols-loaded nanoemulsions in cancer therapy R Tomar, SS Das, VKR Balaga, S Tambe, J Sahoo, SK Rath, ... ACS Applied Bio Materials 7 (4), 2036-2053 , 2024 2024 Citations: 26
Review on Substituted 1,3,4-Oxadiazole and its Biological Activities. JS Rituparna Palit Nikita Saraswat Int. Res. J. Pharm 7 (2), 1-7 , 2016 2016 Citations: 25
Plumbagin Alleviates Intracerebroventricular-Quinolinic Acid Induced Depression-like Behavior and Memory Deficits in Wistar Rats. IM Arora MK, Ratra A, Asdaq SMB, Alshamrani AA, Alsalman AJ, Kamal M, Tomar ... Molecules 27 (6), 1834 , 2022 2022 Citations: 24
Nanosuspension: A novel drug delivery system S Nayak, D Panda, J Sahoo J Pharm Res 3 (2), 241-6 , 2010 2010 Citations: 24
Therapeutic potential of policosanol in the concurrent management of dyslipidemia and non-alcoholic fatty liver disease DKAJ Mandeep K. Arora, Sudhanshu Pandey, Ritu Tomar, Jagannath Sahoo Future Journal of Pharmaceutical Sciences 8 (11), 1-9 , 2022 2022 Citations: 21
A promising approach of dermal targeting of antipsoriatic drugs via engineered nanocarriers drug delivery systems for tackling psoriasis D Tripathi, M Srivastava, K Rathour, AK Rai, P Wal, J Sahoo, RK Tiwari, ... Drug Metabolism and Bioanalysis Letters Formerly: Drug Metabolism Letters 16 … , 2023 2023 Citations: 20
Identification of new resistant sources for bacterial blight in pomegranate BT Priya, BNS Murthy, C Gopalakrishnan, RB Artal, S Jagannath European journal of plant pathology 146 (3), 609-624 , 2016 2016 Citations: 20
Formulation design and optimization of mouth dissolving tablets of levocetirizine hydrochloride using sublimation technique AK Mahapatra, PN Murthy, J Sahoo, S Biswal, SK Sahoo Indian Journal of Pharmaceutical Education and Research 43 (1), 39-45 , 2009 2009 Citations: 20
Physicochemical Properties of Glimepiride in Solid Dispersions with Polyethylene Glycol 20000 PNM OP Baliar Singh. S Biswal, J Sahoo international journal of Pharmaceutical sciences and nanotechnology 2 (2 … , 2009 2009 Citations: 19
Hepatoprotective potential of Trichosanthes dioica roxb in hepatotoxicity induced by simvastatin and its consequences on biochemical and haematological indices RK Gupta, SR Swain, J Sahoo, A Gupta, S Chaudhary Pharmacogn J 10, 720-724 , 2018 2018 Citations: 17
A Review on Traditional and Ethnomedicinal uses of Elaeocarpus ganitrus (Rudraksha) SJ Dixit PK, Bhardwaj M, Nagarajan K International Journal of Pharmaceutical Sciences Review and Research , 2018 2018 Citations: 15
