In vitro Plant Regeneration from Mature Seed Explants of Withania somnifera (L.) Dunal, an Important, Rare and Endangered Medicinal Plant Sape Subba TATA, Geddam JYOTHIRMAYEE, Owk Aniel KUMAR Notulae Scientia Biologicae, 2019 Withania somnifera (L.) Dunal a member of the Solanaceae family, is a traditional medicinal plant commonly known in India as Ashwagandha. It is used for different diseases such as hiccup, cough, rheumatism, tuberculosis, and exhibits excellent antitumor and anti-bacterial activities as well. Direct organogenesis of plants using mature seeds provides faster response and is also a time saving approach, thus the present study was conducted to investigate the optimal concentrations and combinations of plant growth regulators with MS medium for the establishment of an efficient regeneration system in W. somnifera using mature seed as an explant. Therefore, an efficient in vitro protocol for high frequency regeneration has been developed using mature seeds as explant. In the present study, the multiple shoots along with embryogenic callus induction was best seen in MS medium supplemented with BAP (1.5 mg/L) and IAA (0.5 mg/L). Furthermore, MS medium fortified with GA3 (0.3 mg/L) and IBA (3.0 mg/L) alone was suited for shoot elongation and rhizogenesis respectively. The rooted plantlets were hardened and successfully established in the soil. The establishment of a highly reproducible regeneration system would greatly influence the efforts of improvement of the hereby studied medicinal plant species through useful gene transfer technology.
Development of a profused in vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell SS Tata Curr Biol 11, 14-17 , 2020 2020 Citations: 4
In vitro plant regeneration from mature seed explants of Withania somnifera (L.) Dunal, an important, rare and endangered medicinal plant SS Tata, G Jyothirmayee, OA Kumar Notulae Scientia Biologicae 11 (4), 387-391 , 2019 2019 Citations: 9
In Vitro Micropropagation of the Medicinal Plant Physalis angulata L. OA Kumar, S RAMESH, SS TATA Notulae Scientia Biologicae 8 (2), 161-163 , 2016 2016 Citations: 9
Establishment of a rapid plant regeneration system in Physalis angulata L. through axillary meristems OA KUMAR, S RAMESH, SS TATA Notulae Scientia Biologicae 7 (4), 471-474 , 2015 2015 Citations: 14
Assessment of genetic purity of F1 interspecific hybrids of Chilli pepper (Capsicum L.) OA Kumar, SS Tata Asian Journal of Plant Science and Research 5 (5), 47-51 , 2015 2015 Citations: 1
Evaluation of genetic diversity in 21 cultivars of chili pepper (Capsicum annuum L.) using isozyme markers OA Kumar, SS Tata, T Rupavathi European Journal of Experimental Biology 4 (6), 44-49 , 2014 2014 Citations: 11
Genetic Variation of Isozyme Polyphenol Oxidase (PPO) Profiles in Different Varieties of Capsicum annuum L. OA KUMAR, T RUPAVATHI, SS TATA Notulae Scientia Biologicae 5 (4), 454-457 , 2013 2013 Citations: 4
In Vitro Conservation of Withania Somnifera (L) Dunal (Ashwagandha)-A Multipurpose Medicinal Plant OA Kumar, G Jyothirmayee, SS Tata Journal of Asian Scientific Research 3 (8), 852 , 2013 2013 Citations: 8
In vitro plant regeneration from leaf explants of Withania somnifera (L) Dunal (Ashwaganda) - an important medicinal plant OA Kumar, G Jyothirmayee, SS Tata Research in Biotechnology 2 (5) , 2011 2011 Citations: 16
Multiple shoot induction and plant regeneration from nodal explants of Chili Peppers (Capsicum annuum L) OA Kumar, T Rupavati, SS Tata Asian J. Expt. Biol. Sci 2 (3), 517-520 , 2011 2011 Citations: 6
Multiple shoot regeneration from nodal explants of Ashwagandha (L.) Dunal Withania somnifera OA Kumar, G Jyothirmayee, SS Tata Asian J Exp Biol Sci 2 (4), 636-40 , 2011 2011 Citations: 19
Phenolics quantification in some genotypes of Capsicum annuum L OA Kumar, SA Rao, SS Tata Journal of phytology 2 (6) , 2010 2010 Citations: 26
SDS-PAGE seed storage protein profiles in chili peppers (Capsicum L.) OA Kumar, SS Tata Notulae Scientia Biologicae 2 (3), 86-90 , 2010 2010 Citations: 25
Seed Storage Protein Profiles in Cultivars of Capsicum annuum L. OA Kumar, T Rupavati, SS Tata Recent Research in Science and Technology 2 (3) , 2010 2010 Citations: 10
In vitro induction of callusogenesis in chilli peppers (Capsicum annum L.) O Aniel Kumar, S Subba Tata, T Rupavati International Journal of Current Research 3, 42-45 , 2010 2010 Citations: 18
In vitro shoot bud differentiation from hypocotyl explants of chili peppers (Capsicum annuum L.) OA KUMAR, SS TATA Notulae Scientia Biologicae 2 (1), 66-69 , 2010 2010 Citations: 5
Ascorbic acid heterosis in chili peppers (Capisucm L.) OA Kumar, SS Tata Journal of Phytology 2 (2) , 2010 2010 Citations: 8
Cytogenetic studies of F1 Hybrid Capsicum annuum L. x Capsicum chacoense (Hunz). OA Kumar, RC Panda, SS Tata, KGR Rao Journal of Phytology 2 (2) , 2010 2010 Citations: 1
Ascorbic acid contents in chili peppers (Capsicum L.) OA KumAr, SS Tata Notulae Scientia Biologicae 1 (1), 50-52 , 2009 2009 Citations: 107
Cytogenetics of a Spontaneous Fasciated Stem Mutant of Chili Pepper (Capsicum annuum L.) OA Kumar, SS Tata, KGR Rao Cytologia 71 (3), 321-324 , 2006 2006 Citations: 5
MOST CITED SCHOLAR PUBLICATIONS
Ascorbic acid contents in chili peppers (Capsicum L.) OA KumAr, SS Tata Notulae Scientia Biologicae 1 (1), 50-52 , 2009 2009 Citations: 107
Phenolics quantification in some genotypes of Capsicum annuum L OA Kumar, SA Rao, SS Tata Journal of phytology 2 (6) , 2010 2010 Citations: 26
SDS-PAGE seed storage protein profiles in chili peppers (Capsicum L.) OA Kumar, SS Tata Notulae Scientia Biologicae 2 (3), 86-90 , 2010 2010 Citations: 25
Multiple shoot regeneration from nodal explants of Ashwagandha (L.) Dunal Withania somnifera OA Kumar, G Jyothirmayee, SS Tata Asian J Exp Biol Sci 2 (4), 636-40 , 2011 2011 Citations: 19
In vitro induction of callusogenesis in chilli peppers (Capsicum annum L.) O Aniel Kumar, S Subba Tata, T Rupavati International Journal of Current Research 3, 42-45 , 2010 2010 Citations: 18
In vitro plant regeneration from leaf explants of Withania somnifera (L) Dunal (Ashwaganda) - an important medicinal plant OA Kumar, G Jyothirmayee, SS Tata Research in Biotechnology 2 (5) , 2011 2011 Citations: 16
Establishment of a rapid plant regeneration system in Physalis angulata L. through axillary meristems OA KUMAR, S RAMESH, SS TATA Notulae Scientia Biologicae 7 (4), 471-474 , 2015 2015 Citations: 14
Evaluation of genetic diversity in 21 cultivars of chili pepper (Capsicum annuum L.) using isozyme markers OA Kumar, SS Tata, T Rupavathi European Journal of Experimental Biology 4 (6), 44-49 , 2014 2014 Citations: 11
Seed Storage Protein Profiles in Cultivars of Capsicum annuum L. OA Kumar, T Rupavati, SS Tata Recent Research in Science and Technology 2 (3) , 2010 2010 Citations: 10
In vitro plant regeneration from mature seed explants of Withania somnifera (L.) Dunal, an important, rare and endangered medicinal plant SS Tata, G Jyothirmayee, OA Kumar Notulae Scientia Biologicae 11 (4), 387-391 , 2019 2019 Citations: 9
In Vitro Micropropagation of the Medicinal Plant Physalis angulata L. OA Kumar, S RAMESH, SS TATA Notulae Scientia Biologicae 8 (2), 161-163 , 2016 2016 Citations: 9
In Vitro Conservation of Withania Somnifera (L) Dunal (Ashwagandha)-A Multipurpose Medicinal Plant OA Kumar, G Jyothirmayee, SS Tata Journal of Asian Scientific Research 3 (8), 852 , 2013 2013 Citations: 8
Ascorbic acid heterosis in chili peppers (Capisucm L.) OA Kumar, SS Tata Journal of Phytology 2 (2) , 2010 2010 Citations: 8
Multiple shoot induction and plant regeneration from nodal explants of Chili Peppers (Capsicum annuum L) OA Kumar, T Rupavati, SS Tata Asian J. Expt. Biol. Sci 2 (3), 517-520 , 2011 2011 Citations: 6
In vitro shoot bud differentiation from hypocotyl explants of chili peppers (Capsicum annuum L.) OA KUMAR, SS TATA Notulae Scientia Biologicae 2 (1), 66-69 , 2010 2010 Citations: 5
Cytogenetics of a Spontaneous Fasciated Stem Mutant of Chili Pepper (Capsicum annuum L.) OA Kumar, SS Tata, KGR Rao Cytologia 71 (3), 321-324 , 2006 2006 Citations: 5
Development of a profused in vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell SS Tata Curr Biol 11, 14-17 , 2020 2020 Citations: 4
Genetic Variation of Isozyme Polyphenol Oxidase (PPO) Profiles in Different Varieties of Capsicum annuum L. OA KUMAR, T RUPAVATHI, SS TATA Notulae Scientia Biologicae 5 (4), 454-457 , 2013 2013 Citations: 4
Assessment of genetic purity of F1 interspecific hybrids of Chilli pepper (Capsicum L.) OA Kumar, SS Tata Asian Journal of Plant Science and Research 5 (5), 47-51 , 2015 2015 Citations: 1
Cytogenetic studies of F1 Hybrid Capsicum annuum L. x Capsicum chacoense (Hunz). OA Kumar, RC Panda, SS Tata, KGR Rao Journal of Phytology 2 (2) , 2010 2010 Citations: 1