Dr Vijay Singh

@matagujricollege.org

Assistant Professor, Department of Botany, Mata Gujri College, Fatehgarh Sahib
Mata Gujri College, Fatehgarh Sahib

RESEARCH, TEACHING, or OTHER INTERESTS

Plant Science, Agricultural and Biological Sciences, Environmental Science

26

Scopus Publications

Scopus Publications

  • The Influence of Geographical Factors on Polyploidy in Angiosperms with Cartographic Evidence from the Northwestern Himalayas: A Review
    Anupreet Singh Tiwana, Siva Prathap Thummalakunta, Saurabh Gupta, Vijay Singh, and Ramesh Chand Kataria

    Technoscience Publications
    The review paper comprised the impact of geographical and environmental factors on polyploidy and vice versa. The review covers different effects of geographical factors, like spatial isolation, altitude, and local climate on polyploidy, and the behavior of polyploid(s) in abiotic factors, such as temperature and light with a few examples of northwest Himalayas. The paper concludes that polyploid plants behave differently in environmental conditions, as polyploids are more prominent in higher altitudes, colder environments, and nutrient-rich soil than diploid progenitors, but have a mixed distribution in different geographical conditions. Further, polyploidy is more common among perennials than annuals, while niche differentiation depends more on the local environment. The virtual case study results from North and North Western India have been shown with the help of ArcGIS online software. The scrutiny of spatial distribution on maps highlights the fact that polyploidy is still a complex research puzzle with interesting perspectives.

  • Picrorhiza kurroa Royle ex Benth.: Kutki
    Kamalpreet Kaur, Gurpreet Kaur, and Vijay Singh

    Springer Nature Singapore

  • Species of the Genus Rhodiola Found in the Western Himalayas
    Gurpreet Kaur, Kamalpreet Kaur, Vijay Singh, Navneet Kaur Panag, and Preeti Saluja

    Springer Nature Singapore

  • In vitro Antimicrobial Activity of Cucumis L. and Momordica L. against Human Pathogens
    Vijay Singh, Rupinderpal Kaur, Yumnam Devashree, Damanjeet Kaur, and Saurabh Gupta

    Pleiades Publishing Ltd

  • Selenium uptake and immobilization using indigenous Bacillus strain isolated from seleniferous soils of Punjab
    Saurabh Gupta, Bandana, Baby, Abhijit Kumar, and Vijay Singh

    Informa UK Limited

  • Genotoxic effect of fruit extract of wild and cultivated cucurbits using Allium cepa assay
    Rupinderpal Kaur, Yumnam Devashree, Ravindra Kumar, Saurabh Gupta, Mohd. Saleem Wani, and Vijay Singh

    Informa UK Limited
    ABSTRACT Many chemical substances in plants have the potential to be toxins, which may induce harm by altering physiological mechanisms. Allium cepa L. assay is a sensitive test used for the determination of anti-mitotic and genotoxic effects in plant extracts. Genotoxic effects of aqueous, acetone, and methanolic fruit extracts of the cucurbit species were studied using the A. cepa bioassay, of which Cucumis melo var. agrestis L. and Momordica balsamina L. are wild and C. melo. L., C. melo. var. momordica L., M. charantia L., and M. dioica L. are cultivated. Onion bulbs were exposed to fruit extracts for 48 h and onion roots were examined with a microscope to determine their effect on cells. The extracts were slightly mito-depressants with a minimum mitotic index (46.20 ± 0.23%) in methanolic fruit extract of C. melo var. momordica, compared to the aqueous solution, a negative control (64.20 ± 0.12%), and 1 mg∙L−1sodium azide (NaN3) solution (22.53 ± 0.41%). The highest percentage of clastogenic aberrations was in the methanolic extract of C. melo var. momordica (11.8%) and least in the aqueous extract of C. melo var. momordica (2%). Also, mitotic abnormalities, chromosome stickiness, chromatin transfer, chromatin bridges, disorientation of chromosomes, laggards, and polar deviation occurred in root cells of A. cepa. Genotoxicity has been reported in different fruit extracts of cucurbits, which needs to be further investigated in animal models to nullify possible detrimental effects in humans.

  • Swertia chirayita
    Vijay Singh, Vikrant Jaryan, Vikas Sharma, Himanshu Sharma, Indu Sharma, and Vikas Sharma

    Elsevier

  • In vitro pollen germination of Betula utilis, a typical tree line species in Himalayas
    Mohammad Saleem Wani, Maroof Hamid, Younas Rasheed Tantray, Raghbir Chand Gupta, A.H. Munshi, and Vijay Singh

    Elsevier BV

  • Comparison of Key Mineral Elements in Wild Edible Fruits of Ziziphus Mauritiana and Z. Nummularia Using Atomic Absorption Spectrophotometer (AAS) and Flame Photometer
    Amit Sareen, Raghbir Chand Gupta, Gulshan Bansal, and Vijay Singh

    Informa UK Limited
    ABSTRACT Wild fruits, directly or in combination with other foodstuff, are used by many people living in the rural regions of tropical countries to address food and income insecurity. The composition of wild fruits needs to be analyzed and compared with the composition of other traditional fruits. In the present study, we investigated the nutritional status of fruits of two wild species (eight populations) of Ziziphus mauritiana (ZM) and Z. nummularia (ZN) from the different locations of Northwest India, which were denoted as ZM-1, ZM-3, ZM-11, ZN-4, ZN-5, ZN-10, ZN-13, and ZN-14 depending on species and location. ZM-3 emerged the highest moisture content while ZM-11 showed the highest ash content. ZN-14 presented the highest crude fiber and vitamin C contents. ZN-13 exhibited the highest crude fiber content. The concentration of potassium (2456.250 ± 36.044 mg/100 g), sodium (139.257 ± 2.575 mg/100 g), copper (6.733 ± 0.097 mg/100 g), and zinc (2.223 ± 0.085 mg/100 g) was found to be highest in Z. mauritiana, and the amount of calcium (428.350 ± 2.037 mg/100 g), magnesium (110.947 ± 1.086 mg/100 g), iron (9.657 ± 0.244 mg/100 g), manganese (2.623 ± 0.067 mg/100 g), and zinc (2.223 ± 0.085 mg/100 g) was highest in Z. nummularia. Results provide an indication of the nutritional value of the wild edible fruits of Ziziphus mauritiana and Z. nummularia from different populations and can be used to promote these wild fruits for domestication.

  • IAPT chromosome data 30
    Karol Marhold, Jaromír Kučera, Erton Mendonça Almeida, Lânia I.F. Alves, Claudia Araneda‐Beltrán, Carlos M. Baeza, Evgeny V. Banaev, Fabiane R.C. Batista, N. Ivalú Cacho, Olga A. Chernyagina,et al.

    Wiley
    Karol Marhold (ed.), Jaromír Kučera (ed.), Camila Aguiar-Melo, Erton Mendonça de Almeida, Lânia Isis Ferreira Alves, Tatyana V. An’kova, Fernanda Bered, Kallyne Bonifácio, Luana Carvalho, Franco E. Chiarini, Joel M.P. Cordeiro, Mihai Costea, Julio Rubén Daviña, Aleksandr L. Ebel, Allan Falconi-Souto, Cattleya M.P. Felix, Leonardo P. Felix, Aveliano Fernández, Miguel Ángel García, Ignacio García-Ruiz, André dos Santos Bragança Gil, Marcelo Guerra, Luiza Domingues Hirsch, Ana Isabel Honfi, Eliane Kaltchuk-Santos, Sandra Knapp, Rohit Kumar, Vandna Kumari, Juliana Lovo, Reinaldo F.P. Lucena, Enoque Medeiros-Neto, Ana Paula Moraes, Rodrigo Garcia Silva Nascimento, José Achilles Lima Neves, Felipe Nollet, Regina Célia de Oliveira, Andrés Orejuela, Marisa Toniolo Pozzobon, Anna Verena Reutemann, André Rodolfo de Oliveira Ribeiro, Gabriel Hugo Rua, Angeline M.S. Santos, Anádria Stéphanie da Silva, Rosemere Silva, Ronimeire Torres da Silva, Vijay Kumar Singhal, Tatiana T. Souza-Chies, Saša Stefanović, José Francisco Montenegro Valls, Cassiano A.D. Welker & Elena Yu. Zykova

  • Male meiotic studies in six species of pedicularis L. From churdhar and adjoining hills of Sirmaur district (H. P.), India
    Vijay Singh, Raghbir Chand Gupta, Kuljit Kaur, Ramesh Chand Kataria, and Ravindra Kumar

    International Society of Cytology
    Summary The genus Pedicularis L. comprises many medicinal species in Sirmaur district of Himachal Pradesh, the Northwest Himalayan range. In the present study, male meiotic studies have been carried out in six species of the genus and revealed n =8 chromosome number in all taxa. The chromosome number ( n =8) in P. heterodonta and P. punctata are new to the world, while P. oederi reveals the first chromosome report for Indian populations. Besides, a B chromosome and structural heterozygosity were reported for the first time in P. hoffmeisteri and P. pectinata , respectively. Further, meiotic anomalies, like chromatin bridges, interbivalent connections, laggards, un-even gamete formation and reduce pollen viability, were also observed in the studied populations.

  • Male meiotic studies in 29 species of lamiaceae from Sirmaur District of Himachal Pradesh, India
    Vijay Singh, Raghbir Chand Gupta, Kusum Sharma, Vikas Sharma, Manjul Sharma, and Kuljit Kaur

    International Society of Cytology
    Lamiaceae is a requisite depository of many important medicinal herbs, distributed all over the world. The detailed meiotic studies, firstly are helpful in solving taxonomic and evolutionary enigmas and secondly provide best chemotypes for further exploration and conservatory purposes. Male meiosis in 29 species of 54 populations belonging to the 18 genera of Lamiaceae has been undertaken for the first time from Sirmaur district of Himachal Pradesh falling in Western Himalayas. Euploid cytotypes are reported in Ajuga parviflora (n=8x=32), Nepeta eryostachys (n=4x=18) and N. graciliflora (n=4x=18). Besides, B-chromosomes in previously known cytotypes of five species, A. bracteosa (n=32+0-2B), Leucas lanata (n=11+0-1B), Lycopus europeus (n=11+0-1B), Phlomis bracteosa (n=11+0-1B) and Vitex negundo (n=16+0-1B), are reported for the first time. A substantial amount of meiotic abnormalities in the form of chromatin stickiness, cytomixis, chromatin bridges and laggards are reported in the presently worked out population that leads to the high variability in the microsporogenesis, pollen grain size heterogeneity and ultimately, reduce pollen viability. Only, the populations with B-chromosome had a low or negligible frequency of meiotic abnormalities.

  • Male meiosis in 18 species of 07 genera of the tribe Astereae (Asteraceae) from Western Himalaya
    Raghbir Chand Gupta, Henna Goyal, Vijay Singh, and Rajesh Kumar Goel

    Springer Science and Business Media LLC

  • New and varied chromosome reports in twenty-six species of the family asteraceae from cold deserts of the Western Himalaya
    Raghbir Chand Gupta, Henna Goyal, Rajesh Kumar Goel, and Vijay Singh

    International Society of Cytology
    In the present study, 26 species belonging to 17 genera and five tribes of the family Asteraceae collected from natural habitats in cold deserts of the western Himalaya were analyzed on their meiotic events. The chromosome counts are reported for the first time in four species viz. Brachyactis roylei (n=9), Cousinia thomsonii (n=12), Erigeron umbrosus (n=9) and Waldheimia glabra (n=9). The euploid or aneuploid chromosome numbers are reported in Arctium lappa (n=17), Bellis perennis (n=5) and Saussurea heteromalla (n=8). The chromosome counts of n=9 in E. borealis is new to the Indian populations. The chromosome counts in the rest 18 species are new additions from the cold desserts of Lahaul-Spiti district of Himachal Pradesh. The present meiotic study also discloses meiotic abnormalities viz. chromosome stickiness, cytomixis, laggards, etc. leading to the hypo-/or hyperploid meiocytes. These abnormalities further may alter pollen viability by producing unreduced pollen grains and might be responsible for the disparity in the chromosome numbers.

  • Cytogenetic variations in eight species of Saussurea DC. (Asteraceae, Cardueae) from Northwest Himalaya
    V. Singh, R. C. Gupta, R. A. Malik, H. Goyal, S. K. Pradhan, and K. Kaur

    Informa UK Limited
    Abstract The genus Saussurea comprises many species with highly medicinal, religious or other economical values. The chromosome number in the genus ranges from 2n = 24 to 108, based on x = 12, 13, 16, or 17, but the primary base number is still not clear. The present meiotic study cover 8 species (14 populations) of Saussurea from the Northwest Himalayas, and add new or varied cytotypes, as an attempt to solve the enigmatic cytogenetic variations in the genus. The chromosome numbers in Saussurea auriculata (n = 16) is new to the world, while S. costus (n = 17), S. jacea (n = 17) and S. roylei (n = 17) reveal the varied cytotypes at world level. Besides, S. taraxicifolia (n = 16) is a first ever report for Indian populations. The meiotic studies on different populations reveal a substantial amount of meiotic abnormalities in the form of chromosome stickiness, un-oriented bivalents, cytomixis, laggards, etc. leading to the meiocytes with less (aneuploidy) or more (polyploidy) chromosome numbers. These abnormalities may produce unreduced pollen grains and adversely affect pollen viability. The high number of these meiotic abnormalities may be responsible for the chromosome number variation in the genus.

  • Cytomorphological variations and new reports of B-chromosomes in the genus Plantago (Plantaginaceae) from the Northwest Himalaya
    Raghbir Chand Gupta, Vijay Singh, Santosh Bala, Reyaz Ahmad Malik, Vikas Sharma, and Kuljit Kaur

    Elsevier BV

  • Development and characterization of novel microsatellite markers in Trillium govanianum: a threatened plant species from North-Western Himalaya
    Vikas Sharma, Mohammad Saleem Wani, Vijay Singh, Kuljit Kaur, and Raghbir Chand Gupta

    Springer Science and Business Media LLC

  • New chromosome reports in Lamiaceae of Kashmir (Northwest Himalaya), India
    Reyaz Ahmad Malik, Raghbir Chand Gupta, Vijay Singh, Santosh Bala, and Santosh Kumari

    Springer Science and Business Media LLC

  • Development of novel SSR markers for evaluation of genetic diversity and population structure in Tribulus terrestris L. (Zygophyllaceae)
    Kuljit Kaur, Vikas Sharma, Vijay Singh, Mohammad Saleem Wani, and Raghbir Chand Gupta

    Springer Science and Business Media LLC

  • Meiotic and Ethnobotanical Studies on Rheum Species from Kashmir Himalaya
    Shagoon Tabin, Kuljit Kaur, Vijay Singh, Azra Nahaid Kamili, and Raghbir Chand Gupta

    International Society of Cytology
    The Rheum species (R. emodi, R. spiciforme, and R. webbianum) are endangered species and have vanished from lower altitudes. Meiotic studies of the three species show that the polyploidy is reported only in R. webbianum, as it depicts two cytotypes, i.e., diploid (2x) in population from Panzila Top and tetraploid (4x) from Parkhachik and Tangole, of which the diploid cytotype is reported for the first time in this species. Moreover, a diploid cytotype (2x) in R. spiciforme is reported for the first time in India. Further, meiotic course is abnormal in all the studied populations, as all show meiotic abnormalities in the form of cytomixis, chromatin stickiness, unoriented bivalents, chromatin bridges and laggards, leading to abnormal microsporogensis, ultimately affecting pollen viability. Ethnobotanical information depicts the use of different parts of all the Rheum species by local people to control many diseases like piles, constipation, skin eruptions, intestinal infection, wounds, joint pains and also used as food.

  • Meiotic chromosomal studies in family Zygophyllaceae R. Br. from Rajasthan
    Raghbir Chand Gupta, Kuljit Kaur, and Vijay Singh

    The Indian Society of Genetics and Plant Breeding
    Present paper deals with the cytomorphological study of 4 genera and 5 species belonging to family Zygophyllaceae collected from various localities of Rajasthan. Three cytomorphovariants of Tribulus terrestris have been observed with 2n=24, 36 and 48. Peganum harmala with 2n=12 (based on x=6) is reported for the first time from world level, earlier the species is based on x=12. The presence of B-chromosomes also makes the first report of the species from world level. Fagonia cretica (2n=22), Tribulus alatus (2n=24) and Zygophyllum simplex (2n=16) have been cytologically worked out for the first time from India.

  • Anomalous chromosomal behaviour and chromosomal data in some members of subclass gamopetalae from district Hamirpur (H. P.), India
    Raghbir Chand Gupta, Kamlesh Kumari, Kuljit Kaur, and Vijay Singh

    International Society of Cytology
    Summary The present study pertains to the cytomorphology of 19 species belonging to nine families of the subclass Gamopetalae from Hamirpur district (H. P.). Out of these, 5 species are new or varied chromosomes reports. Campanula benthamii (2 n =2 x =28) and Leucas hyssopifolia (2 n =2 x =22) are the first ever chromosome counts for the species. Spilanthes acmella (2 n =6 x =78) and Borreria articularis (2 n =4 x =52) are varied cytotypes. Meanwhile, the B chromosome is reported for the first time in Leucas lanata (2 n =22+0-1B). Almost all of the accessions (with disparity in percentage) show laggards, chromosome stickiness and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double the normal chromosome number. Such meiotic abnormalities produce unreduced pollen grains and reduce pollen viability.

  • Cytogenetic variation among populations of Aster thomsonii C. B. Clarke from district Sirmaur, Himachal Pradesh (India)
    Raghbir Chand Gupta and Vijay Singh

    International Society of Cytology
    Presently, cytomorphological investigations have been carried out in five populations of Aster thomsonii from different altitudinal areas of district Sirmaur (H.P.). The investigations revealed three cytomorphotypes: diploid (2n=18), tetraploid (2n=36) and hexaploid (2n=54). The chromosome count of 2n=54 (hexaploid cytotype) has been reported for the first time at world level. All the populations have anomalous meiotic course and reduced reproductive potential of 72, 81 and 59% for diploid, tetraploid and hexaploid, respectively.

  • Cytology of the genus Artemisia (Anthemidae, Asteraceae) in the Western Himalayas
    Raghbir Chand Gupta, Henna Goyal, and Vijay Singh

    Springer Science and Business Media LLC
    The present study revealed the varied frequency of natural chromosomal abnormalities in 13 populations pertaining to 9 species of the genus Artemisia L. from different localities of Himachal Pradesh (Western Himalaya). Intraspecific chromosome variability has been reported for the first time on worldwide basis in Artemisia vestita (2n = 2x = 36) and from India in A. macrocephala (2n = 2x = 18) and A. scoparia (2n = 2x = 36). Besides, B-chromosomes have been reported here for the first time in A. nilagirica and A. roxburghiana. Most of the populations show anomalous meiotic behaviour resulting in cytomixis, chromosomal stickiness, unoriented bivalents, formation of laggards and bridges which leads to abnormal microsporogenesis, and production of heterogeneous-sized fertile pollen grains along with reduced pollen fertility.

  • Meiotic studies in some species of tribe Cichorieae (asteraceae) from Western Himalayas
    Raghbir Chand Gupta, Henna Goyal, Vijay Singh, and Rajesh Kumar Goel

    Hindawi Limited
    The present paper deals with meiotic studies in 15 species belonging to 6 genera of the tribe Cichorieae from various localities of Western Himalayas. The chromosome number has been reported for the first time inHieracium crocatum(2n=10) andLactuca lessertiana(2n=2x=16). Further, intraspecific variability has been reported for the first time inH. umbellatum(2n=2x=10and2n=6x=54),Tragopogon dubius(2n=2x=14and2n=4x=28), andT. gracilis(2n=2x=14). The chromosome report of2n=2x=10inYoungia tenuifoliais made for the first time in India. Maximum numbers of the populations show laggards, chromosome stickiness, and cytomixis from early prophase to telophase-II, leading to the formation of aneuploid cells or meiocytes with double chromosome number. Such meiotic abnormalities produce unreduced pollen grains and the reduced pollen viability.