Edson Lourenço da Silva
@ifpi.edu.br
Instituto Federal do Piaui
Scopus Publications
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Scopus Publications
MANUELLA F. LEAL, LUIZ RICARDO L. SIMONE, EMERSON S. CASTRO, ORIANNA DOS SANTOS, ANTÔNIA R.V. DA SILVA, KARINA K.S. DANTAS, JOÃO H. DE SOUSA, EDSON L. DA SILVA, TAMARIS G. PINHEIRO, and ANA CAROLINA F. LACERDA
FapUNIFESP (SciELO)
Manuella Feitosa Leal, Luiz Ricardo Lopes de Simone, Ana Carolina Figueiredo Lacerda, Edson Lourenço da Silva, and Tamaris Gimenez Pinheiro
Check List Pensoft Publishers
Corbicula fluminea is an invasive clam originally from Asia that threatens the biodiversity of freshwater communities around the world. It has greatly expanded in Brazil since its first record. We report here the occurrence of C. fluminea from Piauí state, Parnaíba river basin and, provide an assessment of its current distribution in Brazil. The compiled data include 382 records in 22 states and the Federal District, encompassing a variety of ecosystems. These results contribute to a discussion about the invasion and expansion process of C. fluminea in Brazil over time.
MARCELO JOÃO SILVA, Flávia Manoela Galvão Cipriano, Ana Paula de Araújo Vieira, Maria Rita dos Santos Cândido, Tamaris Gimenez Pinheiro, and Edson Lourenço da Silva
EDUFU - Editora da Universidade Federal de Uberlandia
Ameivula is as a new genus of Teiidae family that emerged after extensive revision of species that comprised the former complex of species called Cnemidophorus group. Its species has a wide distribution from the northeast of Brazil to northern Argentina. Cytogenetic studies in the Teiidae family have shown that karyotypical data are important tools in phylogenetic and systematic studies within this group allowing to determine the position of species in the family. Thus, this study aimed to describe the karyotype of Ameivula ocellifera (Spix, 1825) from Picos, Piauí state in the Brazilian Northeast. Specimens were collected from August 2014 to October 2015 using interception traps and pitfalls, mounted randomly along the Caatinga area. The animals were collected and transported to Federal Institute of Piauí, campus Picos, where was carried out all laboratory procedures. Individuals analyzed showed a diploid number of 2n = 50 for both sexes, with karyotype composed by 30 macrochromosomes and 20 microchromosomes of telocentric and subtelocentric types. There were no heteromorphic sex chromosomes in the studied specimens. C-band technique evidenced the heterochromatic blocks in pericentromeric and telomeric regions of chromosomes. The nucleolar organizing regions appeared as a simple unit located at the terminal portion of the long arm of chromosomal pair number 5. The chromosomal characteristics of A. ocellifera analyzed do not show divergences regarding individuals from other regions. However, the nucleolar organizing regions seems to be a good chromosomal marker that permits to distinguish the species already studied.
Marcelo J. da Silva, Ana P. de Araújo Vieira, Flávia M. Galvão Cipriano, Maria R. dos Santos Cândido, Edivaldo H.C. de Oliveira, Tamaris Gimenez Pinheiro, and Edson L. da Silva
S. Karger AG
In this study, we analyzed the karyotype of <i>Salvator merianae</i> (Teiidae) from the Brazilian semiarid region using different cytogenetic markers. Chromosomes were examined by classical (Giemsa and AgNOR staining) and molecular (FISH with ribosomal, telomeric, and microsatellite probes) cytogenetic approaches. <i>S. merianae </i>showed a diploid chromosome number of 2n = 38 (10 biarmed macrochromosomes + 28 microchromosomes). No sex-linked chromosome heteromorphisms were observed<i>. </i>Clusters of 18S/28S rDNA were localized in the terminal region of the long arm of pair 2. In addition to the typical telomeric signals, (TTAGGG)<sub>n</sub> repeats were detected in the pericentromeric region of some macrochromosome pairs, which might indicate the occurrence of chromosomal rearrangements via chromosome fusions. Hybridization signals of the microsatellite probes (GA)<sub>n</sub>, (GAA)<sub>n</sub>, and (GAG)<sub>n</sub> were uniformly distributed across all chromosomes, while (CA)<sub>n</sub>, (CAA)<sub>n</sub>, and (CAC)<sub>n</sub> produced brighter signals in the telomeric and pericentromeric regions of specific chromosome pairs. The comparison with previous studies demonstrates that, despite the wide distribution of <i>S. merianae</i>, the macrostructure organization of the karyotype remained unchanged, showing stability in diploid number and chromosome morphology.
Antonio Ferreira Mendes-Sousa, Leid Daiane Neri de Araújo, Samires Silva de Sousa, Suzane de Carvalho Alencar, Wesesller Almeida de Sousa Júnior, Larisse Maria de Sousa, Suzane Maria da Rocha, José Cleves da Silva Maia, Márcia Maria Mendes Marques, Tamaris Gimenez Pinheiro,et al.
FapUNIFESP (SciELO)
Abstract INTRODUCTION Triatomine bugs are hematophagous insects that are extremely important in public health because they are natural vectors of Trypanosoma cruzi, the causative agent of Chagas disease. In this study, we aimed to assess the occurrence of triatomine species and the natural T. cruzi infection in the Guaribas Valley territory, an endemic region for Chagas disease in northeastern Brazil. METHODS Insects were actively captured from July 2017 to October 2019 in the intra- and peridomiciles of 16 municipalities of the Guaribas Valley territory, in the southeast area of Piauí state. Triatomine species were identified following a taxonomic key, and natural infection was investigated through insects’ fresh feces exams. RESULTS A total of 430 triatomines were collected, including 211 nymphs and 219 adults. Of all collected specimens, 39 (9.1%) were from the intradomiciles and 391 (90.9%) from peridomiciles. Nine species, including two subspecies, could be identified: Triatoma brasiliensis brasiliensis, T. brasiliensis macromelasoma, T. pseudomaculata, T. sordida, T. juazeirensis, T. melanocephala, Panstrongylus lutzi, Rhodnius domesticus, R. nasutus, and R. robustus. T. brasiliensis were the most frequently collected bugs, representing 72% of all the identified insects. None of the examined invertebrates presented flagellate forms of T. cruzi. CONCLUSIONS: This is the first report of T. b. macromelasoma and T. juazeirensis in the Guaribas Valley territory. The persistence of triatomine species in the domiciles in an endemic area for Chagas disease emphasizes the relevance of entomological surveillance and vector control measures in the studied area.
Edson Lourenço da Silva, Adriana Josefa da Rocha, Manuella Feitosa Leal, Orianna dos Santos, João Hemerson de Sousa, Antônia Rafaela Viana da Silva, Karina Ketelen Silva Dantas, Erika Maria Matos Rulim, Emerson Santos Castro, Ana Carolina Landim Pacheco,et al.
FapUNIFESP (SciELO)
Abstract: The pronounced dry season determines the characteristics of the semiarid region of Brazil. Numerous small reservoirs are built to overcome this condition, accumulating multiple uses and causing important changes in the landscape and the local biota. Considering the limited amount of information about the malacofauna of reservoirs, mainly in the Northeast region of the country, this inventory provides a list of mollusks from three important reservoirs located in the city of São Julião, state of Piauí. The collections were carried out monthly between May/2017 and April/2018, sampling a total of 11,149 mollusks, corresponding to the species Melanoides tuberculata (Müller, 1774) (n = 9,724), Biomphalaria straminea (Dunker, 1848) (n = 1,361) and Pomacea lineata (Spix, 1827) (n = 64). The richness of mollusks from the three reservoirs was low, similar to those described in studies conducted in other regions of Northeast Brazil. There was a significant difference in the abundance of M. tuberculata and B. straminea among the studied reservoirs. These environments are not connected and are installed in urban and rural areas, presenting different ecological conditions. The relationship between the human population and the reservoirs, associated with the lack of sanitation, increases the risks of spreading waterborne diseases besides the development of environmental imbalance by the introduction of exotic species.
Edson Lourenço da Silva, Manuella Feitosa Leal, Orianna dos Santos, Adriana Josefa da Rocha, Ana Carolina Landim Pacheco, and Tamaris Gimenez Pinheiro
Pensoft Publishers
Melanoides tuberculata (Müller, 1774) was recorded during field surveys of the malacofauna of Território Vale do Guaribas in Piauí state, northeastern Brazil. Individuals were observed every month (June 2017–September 2018) in both lotic and lentic habitats. Considering that M. tuberculata has high reproductive and adaptative capacities besides being an intermediate host of digenetic trematodes, this invasive species is expected to be a threat to native biodiversity. Furthermore, it may become a serious environmental problem considering the multiple uses of the water bodies where it lives.
Patrícia Parise-Maltempi, Edson da Silva, Willem Rens, Frances Dearden, Patricia CM O’Brien, Vladimir Trifonov, and Malcolm A Ferguson-Smith
Springer Science and Business Media LLC
Rafael Splendore de Borba, Edson Lourenço da Silva, Josi Margarete Ponzetto, Allan Pierre Bonetti Pozzobon, Liano Centofante, Anderson Luis Alves, and Patrícia Pasquali Parise-Maltempi
FapUNIFESP (SciELO)
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the Mato Grosso wetland were sampled. The ATP sintetase (subunits 6 and 8) gene was completely sequenced, the mean of nucleotide base composition in the sequences was 31.2% (T), 30.2% (C), 26.9% (A) and 11.9% (G), with no gene saturation. The population analysis in the TCS program generated a network with six haplotypes (A to F), where the ancestral haplotype (A) has a frequency of 25% and is composed by individuals from Cuiabá and Paraguay Rivers. The phylogenetic analysis showed the occurrence of two mtDNA lineages (1 and 2), the distance observed between the two lineages was 0.6%. The phylogenetic and phylogeographic results as well as the negative values of Fst for some populations, indicate a possible occurrence of gene flow among the analyzed populations. These results highlights the importance of flood pulse existent on wetland as a vehicle that permits a temporary connection among isolated population maintaining the species genetic variability.
Edson Lourenço da Silva, Rafael Splendore de Borba, and Patrícia Pasquali Parise-Maltempi
Springer Science and Business Media LLC
Abstract Background Members of the Anostomidae family provide an interesting model system for the study of the influence of repetitive elements on genome composition, mainly because they possess numerous heterochromatic segments and a peculiar system of female heterogamety that is restricted to a few species of the Leporinus genus. The aim of this study was to isolate and identify important new repetitive DNA elements in Anostomidae through restriction enzyme digestion, followed by cloning, characterisation and chromosome mapping of this fragment. To identify repetitive elements in other Leporinus species and expand on studies of repetitive elements in Anostomidae, hybridisation experiments were also performed using previously described probes of LeSpe I repetitive elements. Results The 628-base pair (bp) LeSpe II fragment was hybridised to metaphase cells of L. elongatus individuals as well as those of L. macrocephalus, L. obtusidens, L. striatus, L. lacustris, L. friderici, Schizodon borellii and S. isognathus. In L. elongatus, both male and female cells contained small clusters of LeSpe II repetitive elements dispersed on all of the chromosomes, with enrichment near most of the terminal portions of the chromosomes. In the female sex chromosomes of L. elongatus (Z2,Z2/W1W2), however, this repeated element was absent. In the remaining species, a dispersed pattern of hybridisation was observed on all chromosomes irrespective of whether or not they were sex chromosomes. The repetitive element LeSpe I produced positive hybridisations signals only in L. elongatus, L. macrocephalus and L. obtusidens, i.e., species with differentiated sex chromosomes. In the remaining species, the LeSpe I element did not produce hybridisation signals. Conclusions Results are discussed in terms of the effects of repetitive sequences on the differentiation of the Anostomidae genome, especially with respect to sex chromosome evolution. LeSpe II showed hybridisation patterns typical of Long Interspersed Elements (LINEs). The differential distribution of this element may be linked to sex chromosome differentiation in L. elongatus species. The relationship between sex chromosome specificity and the LeSpe I element is confirmed in the species L. elongatus, L. macrocephalus and L. obtusidens.
E.L. da Silva, A.F. Busso, and P.P. Parise-Maltempi
S. Karger AG
Chromosome mapping and studies of the genomic organization of repetitive DNA sequences provide valuable insights that enhance our evolutionary and structural understanding of these sequences, as well as identifying chromosomal rearrangements and sex determination. This study investigated the occurrence and organization of repetitive DNA sequences in <i>Leporinus elongatus</i> using restriction enzyme digestion and the mapping of sequences by chromosomal fluorescence in situ hybridization (FISH). A 378-bp fragment with a 54.2% GC content was isolated after digestion with the <i>Sma</i>I restriction enzyme. BLASTN search found no similarity with previously described sequences, so this repetitive sequence was named Le<i>Sma</i>I. FISH experiments were conducted using <i>L. elongatus</i> and other Anostomidae species, i.e. <i>L. macrocephalus,</i><i>L. obtusidens, L. striatus, L. lacustris, L. friderici, Schizodon borellii, S. isognathus</i>, and <i>Abramites hypselonotus</i> which detected signals that were unique to male and female <i>L. elongatus</i> individuals. Double-FISH using Le<i>Sma</i>I and 18S rDNA showed that Le<i>Sma</i>I was located in a nucleolus organizer region (NOR) in the male and female metaphases of <i>L. elongatus</i>. This report also discusses the role of repetitive DNA associated with NORs in the diversification of Anostomidae species karyotypes.
E. Lourenço da Silva, D. Piscor, J. M. Ponzeto, L. Centofante, A. L. Alves, and P. P. Parise-Maltempi
Springer Science and Business Media LLC
Rafael Splendore de Borba, Edson Lourenço da Silva, Ana Carolina Sales Pacheco, Patricia Pasquali Parise-Maltempi, and Anderson Luis Alves
Springer Science and Business Media LLC
Patricia Parise-Maltempi, Edson Silva, Rafael de Borba, Liano Centofante, and Carlos Miyazawa
Pensoft Publishers
Abstract Thoracocharax stellatus (Characiformes, Gasteropelecidae) is a small Neotropical species of fish, widely distributed in several rivers of South America. Evidence for karyotype heteromorphysm in populations from different geographical regions has been reported for this species. In this way, populations of Thoracocharax stellatus from the Paraguay River basin were cytogenetically characterized and the results were compared with other studies performed in the same species but from different basins. The results showed a diploid number of 2n = 54 for Thoracocharax stellatus, with chromosomes arranged in 6 metacentric (m), 6 submetacentric (sm), 2 subtelocentric (st) and 40 acrocentric (a), for both sexes, with a simple Nucleolus Organiser Region (NOR) system reported by the techniques of silver nitrate impregnation and fluorescent in situ hybridisation (FISH) using 18S rDNA sequences as probe. The distribution of constitutive heterochromatin, observed by the C-band technique and Chromomycin A3 staining showed great similarity among the analyzed populations and consists mainly of discrete blocks in the pericentromeric and telomeric regions of most chromosomes. The presence of female heterogamety was also observed indicating a ZZ/ZW system with W chromosome almost totally heterochromatic. The results also show cytogenetic diversity of the group and are useful to understand the mechanisms of karyotype evolution of the family.
Edson Lourenço da Silva, Liano Centofante, and Carlos Suetoshi Miyazawa
FapUNIFESP (SciELO)
Foram utilizadas análises de variáveis canônicas livres de tamanho com o objetivo de investigar os padrões de variação morfológica entre populações de Thoracocharax stellatus em bacias hidrográficas Sul-Americanas: rios das bacias Araguaia-Tocantins e Paraguai do Brasil e Orinoco na Venezuela. Distinções entre as amostras do Araguaia-Tocantins e Orinoco foram observadas, com leve sobreposição dessas populações com as amostras da bacia do Paraguai. Os caracteres morfométricos que são os principais responsáveis por esta diversificação são comprimento da cabeça e comprimento da nadadeira dorsal. Os mecanismos que podem estar atuando nesta variação geográfica entre populações de T. stellatus são discutidos.