Agricultural and Biological Sciences, Soil Science, Plant Science, Agronomy and Crop Science
6
Scopus Publications
Scopus Publications
Exploration of Cellulolytic Microbial Consortium as Biodecomposer of Coffee Fruit Skin Waste Eka Wisdawati, Nildayanti ., Aksal Mursalat, Islah Fauliah, Hasmi Aprelia Pakistan Journal of Biological Sciences, 2025 Background and Objective: Coffee fruit skin waste contains high cellulose leading to difficulty in degrading them naturally which finally creates environmental pollution. This research is aimed at finding out the compatible cellulolytic microbe in degrading cellulose content of coffee fruit skin waste. Materials and Methods: Media Carboxymethyl Cellulose (CMC) was used to select cellulolytic fungi. These fungi were tested through a consortium test on media to check out whether the microbial consortium was compatible or not. Furthermore, the ability of microbial consortium in degrading lignocellulose of coffee fruit skin waste was examined. Results: It was found that there were five singular isolates and seven consortium isolates able to degrade cellulose in variative cellulolytic index ranging from 0.43-2.75. In the cellulolytic compatibility test of the seven consortium isolates, there were two compatible consortium isolates with a CCI amount above 1, meanwhile, the consortium test result which was inoculated in PDA media, demonstrated the presence of six consortium isolates of non-synergistic due to the forming of inhibition zone in each isolate. A positive interaction happened between Trichoderma asperellum and Aspergillus japonicus (TA/AJ). Likewise, at the cellulolytic degradation capability test, consortium TA/AJ can degrade cellulose faster compared to using one isolate. Conclusion: The consortium of TA/AJ was the best and proved to be compatible in degrading lignocellulolytic of coffee fruit skin waste.
Phosphate solubilization potential of rhizosphere fungi isolated from satoimo taro plant E Wisdawati, H Widyastuti Iop Conference Series Earth and Environmental Science, 2023 Phosphorus is an essential nutrient required by plants for their growth and development. Phosphates bioavailability in nature is approximately 95-99% but insoluble, therefore the plant can not simply use them. The alternative to overcome phosphate deficiency is by utilizing phosphate solubilizing fungi. This research is aimed at selecting and characterizing phosphate solubilizing fungi in rhizosphere of saitomo taro (Colocasia esculenta var antiquorum). The test on isolate ability to solubilize phosphate was conducted through qualitative and quantitative. Qualitative was carried out by counting index of phosphate solubilization on solid Pikovskaya while quantitative was through counting concentration of phosphate solubilization through spectrophotometry at wave length 693 nm. The result of qualitative test showed that the highest index of phosphate solubilisation was 1.08 at isolate RTP25 while quantitative demonstrated that isolate RTP16 released highest concentration of phosphate solubilization with the amount 12.78 µgl−1. Isolate RTP 25 (by Trichoderma sp) and RTP16 (by Aspergillus sp) be potential to be developed as phosphate biofertilizer.
Comparation of two pruning methods toward arabica coffee seedling growth E Wisdawati, M Yusuf, E Tambaru, A Pasareang Iop Conference Series Earth and Environmental Science, 2023 Coffee production success in Indonesia was strongly affected by culture technique, for instance, proper pruning. Pruning Arabica coffee seedling is one of techniques to boost productivity by cutting the plant shoots and stems. This study aims to determine the effectiveness of shoot pruning and 50% pruning on the growth of Arabica coffee seedlings. Data processing was carried out by statistical methods to compare or test the average data of two paired populations using the T test at a level of 0.05. Parameters observed were plant height, number of leaves, stem diameter and number of branches. The results of the arabica coffee seedling experiment with two treatments of pruning on the shoots pruning showed that the average plant height, number of leaves, and number of branches, was higher than pruning at the 50% pruning. Meanwhile, pruning 50% of stem diameter showed a higher average than pruning of shoots. It can be concluded that pruning of Arabica coffee seedlings, especially shoot pruning, has a better effect on the growth of arabica coffee seedlings.
Screening and identification of cellulolytic fungi at rhizosphere of safira taro plant E Wisdawati, T Kuswinanti, A Rosmana, A Nasruddin Iop Conference Series Earth and Environmental Science, 2021 Agriculture waste and pathogen cell wall of plant contain a lot of cellulose which are very difficult to degrade. One of the ways to hydrolyse them is through utilizing cellulase, an enzyme produced from secondary metabolite of cellulolytic fungi. This research was aimed at isolating fungi from rhizosphere of safira taro plant and testing the isolate capability to produce cellulase enzyme to be able to act as decomposer and biocontrol of the plant. The research methods are isolating rhizosphere fungi, testing isolate capability in producing cellulase enzyme and identifying potential isolate producing cellulolytic fungi. It obtained 10 fungi isolates, 4 were negative and 6 positive in producing cellulase enzyme with cellulolytic index 1.16 – 1.66. The highest index was RTB14 isolate which belongs to Aspergillus flavus.
Production of Indol-3-Acetic Acid (IAA) by fungal isolates of taro (Colocasia esculenta var. antiquorum) rhizosphere E Wisdawati, T Kuswinanti, A Rosmana, A Nasruddin Iop Conference Series Earth and Environmental Science, 2020 Many taros (Colocasia esculenta var. antiquorum) grow in Sulawesi due to their large scale acceptability and high return per unit area, but their productivity is still low. In this study, fungi producing indol-3-acetic acid (IAA) were selected and characterized from the taro rhizosphere with the aim that it can be applied in stimulating the growth and production of this plant. By analyzing the content of IAA in the medium of fungi isolated, 12 fungal isolates were found producing this compound. The highest level of IAA is provided by isolate ETR 33 (8.89 ppm), ETR 29 (8.21 ppm), and ETR 5 (7.82 ppm). These isolates were identified as Fusarium, Trichoderma, and Aspergillus, respectively. These data that three strains of fungi were identified and characterized as the producer of IAA and potentially be used to develop a bio-fertilizer to increase the productivity of taro.
