mohammad sadegh gholami farashah
@ssu.ac.ir
Assistant Professor, Department of Biology and Anatomical Sciences, Faculty of Medicine
Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
RESEARCH, TEACHING, or OTHER INTERESTS
Anatomy, Histology, Embryology, Cell Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Mohammad Sadegh Gholami Farashah, Maryam Javadi, Jafar Soleimani Rad, Seyed Kazem Shakouri, Solmaz Asnaashari, Siavoush Dastmalchi, Sadeneh Nikzad, and Leila Roshangar
Maad Rayan Publishing Company
Purpose: Exosomes are natural nanoparticles that participate in intercellular communication through molecular transport. Recently, due to their membrane vesicular structure and surface proteins, exosomes have been used extensively in the research field of drug delivery. Osteoporosis is an inflammation in which the cellular balance of bone tissue is disturbed that reduces bone density and making bone prone to abnormal fractures with small amount of force. Utilizing estrogen is one of the main therapeutic strategies for osteoporosis. Despite the positive effects of estrogen on bone tissue, changes in the natural estrogen levels of the body can cause a number of diseases such as different types of cancer. Therefore, designing a therapeutic system which controls more accurate tissue targeting of estrogen seems to be a rational and promising practical approach. Methods: In this study, bone marrow mesenchymal stem cells (BMMSCs)-derived exosomes were loaded by estradiol using two different methods of drug loading, namely incubation and sonication methods and then the survival effects of the drug loaded exosomes on BMMSCs was investigated. Results: Examination of size, shape, and surface factors of exosomes in different states (pure exosomes and drug-loaded exosomes) showed that the round morphology of exosomes was preserved in all conditions. However, the particles size increased significantly when loaded by sonication method. The increased survival of BMMSCs was noted with estradiol-loaded exosomes when compared to the control group. Conclusion: The results suggest that estradiol-loaded exosomes have potential to be used as nano-drug carriers in the treatment of osteoporosis.
M. Naghibi, Hamid Tayefi Nasrabadi, Jafar Soleimani Rad, A. Garjani, Mohammad Sadegh Gholami Farashah and Daryoush Mohammadnejad
Background: In many diabetic patients, spermatogenesis complications are frequent causing infertility problems. This study aimed to demonstrate the effect of Forskolin on male reproductive dysfunction caused by type 2 diabetes. Materials and Methods: In this experimental study, type 2 diabetes was induced by a high-fat diet (HFD) for one month and then a low single dose injection (35 mg/kg) of streptozotocin (STZ) in Wistar rats. After 72 hours, rats with more than 200 mg/dl of blood glucose were considered type 2 diabetic rats. Forty rats (200-250 g) were divided into four groups (n=10) including group 1 (G1): rats with normal diet and buffer citrate (STZ solvent) injection, group 2 (G2): control type 2 diabetic rats with HFD and STZ injection, group 3 (G3): type 2 diabetic rats received phosphate buffer saline (PBS) as Forskolin solvent, and group 4 (G4): Forskolin treated diabetic rats (10 mg/kg) for 1 month. Results: In comparison to control group, in diabetic groups (G2 and G3) some parameters are increased significantly: The blood glucose (P=0.00078), testicular malondialdehyde (MDA) level and body weight (P=0.00009) and Bax gene expression (P=0.00007). Unlike, some parameters are decreased significantly: The serum level of testosterone (P=0.0009), testicular superoxide dismutase (SOD, P=0.00007) and glutathione peroxidase (GPX) levels (P=0.00008), sperm concentration (P=0.00008), motility (P=0.00009), normal morphological sperm (P=0.00008) and Bcl-2 gene expression (P=0.00009). However, in Forskolin treated group (G4) the parameters stayed close to control values that was significantly (P=0.00007) higher than in G2 and G3 groups. Therefore, treatment with Forskolin significantly improved these abnormal changes in Forskolin-treated group. Conclusion: Our study demonstrates that Forskolin is an effective antidiabetic agent, which significantly improves sperm concentration, testosterone levels, and antioxidant activity in diabetic rats.
Mohammad Sadegh Gholami Farashah, Amirhossein Mohammadi, Maryam Javadi, Jafar Soleimani Rad, Seyed Kazem Shakouri, Shahla Meshgi, and Leila Roshangar
Springer Science and Business Media LLC
Mohammad Sadegh Gholami Farashah, Maryam Javadi, Amirhossein Mohammadi, Jafar Soleimani Rad, Seyed Kazem Shakouri, and Leila Roshangar
Springer Science and Business Media LLC
Mehran Naghibi, Hamid Tayefi Nasrabadi, Jafar Soleimani Rad, Mohammad Sadegh Gholami Farashah, and Daryoush Mohammadnejad
Hindawi Limited
This study aimed to investigate the effects of metformin and forskolin independently and in combinations on the sperm quality parameters and sexual hormones of diabetic male rats. Fifty adult male rats were divided randomly into five identical groups, and diabetes mellitus was induced to the rats, except for the rats in the control group, using a high‐fat diet and injection of Streptozotocin. Daily administration of metformin and forskolin independently and in combinations were performed for 8 weeks in different groups. Sperm quality parameters (including sperm count, morphology, sperm motility and Johnson score), testosterone, blood sugar level, Bax to Bcl‐2 ratio mRNA expression level and oxidative stress levels were measured and compared between the investigated groups. Treating diabetic rats with metformin and forskolin resulted in significant improvement in sperm quality parameters, increased testosterone levels, reduced oxidative stress in blood and testicular tissue, and decreased blood sugar, and Bax to Bcl‐2 ratio level. Although the combination of metformin with forskolin had a higher effect in some parameters such as testosterone levels compared to treatment with metformin or forskolin alone, this combination had not shown a synergistic effect in all the sperm quality parameters. Metformin and forskolin are effective anti‐diabetic agents, which significantly improve the sperm quality and sexual hormone levels in diabetic rats. Combining metformin and gorskolin resulted in significantly better testosterone level and antioxidant activity in blood serum without significant effect on sperm quality of diabetic rats.
Maryam Javadi, Jafar Soleimani Rad, Mohammad Sadegh Gholami Farashah, and Leila Roshangar
Springer Science and Business Media LLC
Maryam Javadi, Jafar Soleimani Rad, Maryam Pashaiasl, Mohammad Sadegh Gholami Farashah, and Leila Roshangar
Elsevier BV
Raana Sarvari, Peyman Keyhanvar, Samira Agbolaghi, Mohammad Sadegh Gholami Farashah, Amirhouman Sadrhaghighi, Mohammad Nouri, and Laila Roshangar
Informa UK Limited
Abstract Shape-memory characteristics shed light on materials science and open horizons for accessing to unconventional functions in material categories subsuming metals, polymers, and ceramics. Biomedical fields manifest great interest in adopting shape-memory materials for unconventional properties, specifications, and well-defined mechanical properties. In spite of the mentioned advantages, their biocompatibility for long-term and permanent applications has not yet been comprehensively distinguished. This article summarizes the most common clinical applications of shape memory alloys and polymers, e.g., tissue engineering, drug delivery, hydrogels, orthodontic wires/distracters, artificial discs, orthopedic staples, atrial septal occlusion devices, neurosurgical stents, etc., and also addresses their critical biocompatibility issues. Graphical Abstract
Raana Sarvari, M. Nouri, L. Roshangar, Mohammad Sadegh Gholami Farashah, A. Sadrhaghighi, S. Agbolaghi and Peyman Keyhanvar
Development of biopolymers possessing both biodegradable and electrically conducting properties has attracted a huge interest in the biomedical field. These systems have some benefitials in wound healing and reducing the long-term health risks. In this study, the pectin-polycaprolactone (Pec-PCL) copolymers were synthesized by ring-opening polymerization. Subsequently, the solutions of the synthesized Pec-PCL and homopolyaniline (H-PANI) were blended in various ratios and their conductivity properties were measured by cyclic voltammetry and the composition of 80:20 was selected for electrospinning process because of the suitable electroactive behavior and biodegradability. The morphology, biocompatibility, hydrophilicity, and mechanical properties of the nanofibers were thoroughly investigated. Resulted scaffolds represented a porous structure with large surface area (110–130 nm) and Young’s modulus of 1615 ± 32 MPa, which imitated the natural microenvironment of extra cellular matrix (ECM) to regulate the cell attachment, proliferation and differentiation. The results demonstrated that these electrospun nanofibers could be potentially applied in biomedical such as tissue engineering.
Susan Mohammadi, Morteza Abouzaripour, Nastaran Hesam Shariati, Fardin Fathi, Mohammad Sadegh Gholami Farashah, and Mohammad Bakhtiar Hesam Shariati
Knowledge E
Metastasis is responsible for most cancer-related morbidity and mortality. In principle, metastasis is the spread of cancer from the primary site to distant tissues. Pathological consistency may be compromised during metastasis. We report the case of a 55-year-old man with MRI images of the dorsal spine showing the effects of a fifth dorsal collapse. He was referred to our hospital because of back pain, imbalance, inability to walk, and weakness of the lower limb. The histological features of bone tumors were corresponding with cell carcinoma, and bone damage was considered metastatic from a site in the lung. His general situation gently diminished, and He died during radiotherapy.
Reza Asghari, Susan M Mohammadi, Fardin Fathi, Nastaran Hesam Shariati, Mohammad Sadegh Gholami Farashah, and Mohammad Bakhtiar Hesam Shariati
Knowledge E
Splenic artery aneurysm (SAA) is rare, often with no sign patient, discovered accidentally in ultrasonography and imaging studies. A healthy 45-year-old woman was referred to us by abdominal pain in the epigastric region-imaging showed a large mass located between the spleen, stomach, and pancreas. CT scan showed two true aneurysms of a 4 mm and 12 mm diameter in the middle third and distal part of the splenic artery. SAAs that are lesser than 2cm can be controlled; however, our patient was given an open surgery, and splenectomy with the removal of the aneurysm has done.
Hamed Shoorei, Majid Banimohammad, Maziar M Kebria, Mohammad Afshar, Mohammad MH Taheri, Majid Shokoohi, Mohammad SG Farashah, Mina Eftekharzadeh, Omid Akhiani, Robert Gaspar,et al.
Frontiers Media SA
In vitro follicular culture systems provide optimal culture models for research about the physiology of the ovary and support the clinical practices to achieve competent mature oocytes for in vitro fertilization. In vitro maturation of preantral follicles makes it possible to study the effects of therapeutic agents on various conditions or disorders of the ovary. Nowadays, preventive bioflavonoids against cancer, hypercholesterolemia, fatty liver, or a variety of toxic agents are in focus. The aim of this study was to design and investigate the impacts of different concentrations of hesperidin, a glycoside flavonoid, on the in vitro preantral follicle growth and maturation in the three-dimensional (3D) culture system which was made with sodium alginate. Preantral follicles (n = 1363) were mechanically isolated from immature mice ovaries, then, after capsulating, they were randomly divided into four groups: the control group received no concentration of hesperidin, and three experimental groups were supplemented with 10, 22.5, and 50 µmol/L of hesperidin. All groups were cultured for 12 days. At the end of the culture period, the percentage of survival rate, antrum formation, obtained metaphase II oocytes, and the secretion of 17β-estradiol and progesterone were significantly higher in the group Hesp 50 (50 µmol/L hesperidin). Moreover, the mean average of follicular diameter cultured in the group Hesp 50 was also increased and the mRNA expression levels of PCNA, FSH-R, and Bcl-2 genes were higher, while Bax mRNA expression was significantly reduced compared with the other groups. Follicles cultured in the presence of 50 µmol/L of hesperidin had a higher fertilization rate and embryo development. Adding hesperidin at the concentration of 50 µmol/L to the culture medium resulted in higher follicular growth and maturation and increased the rate of in vitro fertilization and embryo development. Impact statement It has been stated that hesperidin has many pharmacological effects, such as anti-inflammatory and antioxidant effects, antimicrobial activity, and anti-carcinogenic activity; but hesperidin and its derivatives have been under investigation as anti-fertility factors for a very long time. However, our results show that hesperidin can improve mice follicular growth and maturation during in vitro 3D culture. Hesperidin as an antioxidant factor could enhance the mRNA expression levels of two important genes involved in folliculogenesis, PCNA, and FSH-R. Our results prove for the first time that hesperidin not only has deleterious effects on follicular development but can also increase rates of in vitro fertilization and embryo development.
Mohammad Sadegh Gholami Farashah, Nastaran Hesam Shariati, Reza Asghari, Susan Mohammadi, Mohammad Amin Dolatkhah, and Mohammad Bakhtiar Hesam Shariati
Knowledge E
Abstract- Pulmonary sequestration is a relatively rare congenital malformation characterized by an abnormal mass of dysplastic lung tissue. This mass is separated from the bronchopulmonary tree and vascularized by an aberrant systemic artery. Pulmonary sequestration common symptoms are chest pain, persistent dry cough, shortness of breath, hemoptysis, and recurrent attractions of pneumonia. We report a case of a 45-year-old man who suffered from recurrent cough for one year and bleeding with pain in the left chest. He was eventually diagnosed with intralobar pulmonary sequestration. Surgical removal of the sequestration generally has good outcomes.