Fatemeh Shafiee

Scopus Publications

Fabrication of a novel Ganoderma lucidum-integrated polyvinyl alcohol/sodium alginate hydrogel for advanced wound healing applications
Faezeh Yazdanparast, Mehdi Mehdikhani, Afsaneh Yegdaneh, Fatemeh Shafiee, Melika Babaei, et al.
Heliyon, 2026
Peptide and Protein Tags for the Half-Life Extension of Therapeutic Agents: A Review Article
Sorour Aboutalebi, Fatemeh Najafi, Negin Yazdizadeh, Fatemeh Shafiee
Protein Journal, 2026
Evaluation of the Anti-melanogenic Effect of Aqueous and Hydroalcoholic Extracts of Nasturtium Officinale on the B16F10 Cell Line
Mohaddese Heydari, Masoud Sadeghi-Dinani, Fatemeh Shafiee
Avicenna Journal of Medical Biotechnology, 2026
Background: This project aimed to evaluate the anti-melanogenic characteristics of Nasturtium officinale (N. officinale) by assessing the impact of both aqueous and hydroalcoholic extracts on the inhibition of cellular and mushroom tyrosinase enzymes, as well as the suppression of the melanin synthesis in B16F10 melanoma cells.Methods: The aerial components of N. officinale were subjected to extraction using distilled water: ethanol (7:3) through the maceration technique. The extract’s phenolic compounds were quantified employing the Folin-Ciocalteu method. The evaluation of the safety profile of the extracts on B16F10 cells was done by the MTT assay. Subsequently, the melanin concentration in B16F10 cells, alongside the inhibitory effects on both mushroom and cellular tyrosinase, was assessed following treatment with the aforementioned extracts.Results: The aqueous and hydroalcoholic extracts exhibited no significant toxicity on B16F10 when compared to Phosphate-Buffered Saline (PBS). Additionally, there was no notable difference in the cytotoxic effects of extracts on the B16F10 cell line. Both extracts resulted in inhibition of cellular and mushroom tyrosinase, along with a decrease in melanin levels in B16F10 in a concentration-dependent manner. Ultimately, the total phenolic content in the aqueous and hydroalcoholic extracts was found to be approximately 14 and 30 mg/g of gallic acid, respectively.Conclusion: This in vitro investigation offers evidence supporting the skin brightening properties of N. officinale as an anti-melanogenic agent. Given its safety profile and absence of toxic effects on melanoma cells, it may be incorporated into the formulation of skin-brightening products following preclinical tests.
Intein-Mediated Production and Anti-tumor Evaluation of DT386-BR2 in Animal Model of Human Breast Cancer
Sajad Maghareh-Dehkordi, Taha Safi Ghahderijani, Melika Kolahdoozan, Fatemeh Moazen, Ardeshir Talebi, et al.
International Journal of Peptide Research and Therapeutics, 2026
Mutant soluble Interleukin-6 receptor as a potential agent for inhibiting cell migration and inducing apoptosis in a breast cancer cell line
Maryam Sadat Mousavizadeh-Marvast, Mina Mirian, Fatemeh Shafiee
Molecular Biology Reports, 2025
Preparation of Pluronic F127 hydrogel loaded with BIF1-iRGD recombinant protein for its targeted anti-cancer effects
Zahra Tamizifar, Abbas Jafarian-Dehkordi, Somayeh Taymouri, Fatemeh Shafiee
Research in Pharmaceutical Sciences, 2025
Background and purpose: Pluronic F127-based hydrogel is a fair formulation for increasing the protein stability and half-life without decreasing its biological activity. The present study aimed to prepare FP127 hydrogel loaded with the recombinant BIF1-iRGD protein as a new immunotoxin with targeting potential of cancer cells. Experimental approach: BIF1-iRGD in 19% w/v of FP127 was prepared by the cold method, and its in vitro release was determined. MTT and flow-cytometry assays were performed to evaluate the cytotoxic and apoptotic effects of BIF1-iRGD and BIFl-iRGD-hydrogel against 4T1 cells. The tumor size of 4T1 Balb-C mice was evaluated, and H&E staining was used for histopathology evaluation. Findings/Results: BIFl-iRGD release followed the first-order model. The toxicity of BIFl-iRGD was less than that in the formulation as a hydrogel after 48 and 72 h of treatment. The null hydrogel showed no toxicity compared to the untreated cells. After 24 h, cells treated with BIFl-iRGD-hydrogel and BIFl-iRGD around their 48-h IC 50 value developed apoptosis at about 55% and 35%, respectively. The tumor size decreased over time in the treated mice during the 20 days after the last injection. Also, no significant difference was observed between the effectiveness of the two groups. Tumor sections of mice treated with BIF1-iRGD and BIF1-iRGD-hydrogel had necrotic parts of about 65% and 70%, respectively. Conclusion and implications: BIF-iRGD-hydrogel showed in vivo anticancer effects with increased toxicity in comparison to the native protein. However, the investigation of protein distribution and probable cytotoxic effect on vital organs must be noted.
Characterization of enteric-coated capsules filled with colchicine loaded zein nanoparticles for colon delivery
Somayeh Taymouri, Somayeh Mirseyfifard, Fatemeh Shafiee
Therapeutic Delivery, 2025
Fractionation of Allium giganteum Effective Extract on Gastrointestinal Neoplasms Cell Lines for Its Anti-Proliferative Properties
Masoud Sadeghi Dinani, Mohammadreza Ghanbarzadeh, Fatemeh Shafiee
Advanced Biomedical Research, 2025
Background: This study was designed to investigate the various extracts of Allium giganteum in term of their cytotoxic effects on gastric and colon cancer cell lines and then fractionation of extract with the most desired effects on cancer cells in comparison to the normal cell line. Materials and Methods: The A. giganteum air-dried bulbs were extracted in four solvents including aqueous, butanol, chloroform: methanol (9:1), and dichloromethan extracts. Evaluating the cytotoxicity of extracts were done by MTT assay against HT-29, KATO-III, and HUVEC cell lines. The extract with The most cytotoxicity on cancer cells and lower toxicity on normal cell line was subjected to the fractionation by MPLC using a RP-18 silica gel column and in the next stage and the cytotoxicity of resulted fractions were determined by MTT assay. Results: Chloroform: methanol extract was the safest extract on HUVEC cells with great cytotoxic effects against cancer cell lines (IC50 17.3 and 23 µg/mL, for KATO-III and HT-29, respectively). The results of MTT assay for 7 fractions of the chloroform: methanol extract showed that 5th fraction (E) was the most cytotoxic fraction (IC50 9 and 11.2 µg/mL for HT-29 and KATO-III, respectively). TLC and NMR analysis of the fractions suggested the presence of steroidal saponins as the main constituents. Conclusion: Significant cytotoxic effects of Allium giganteum was established against GI cancer cell lines especially fractions rich in steroidal saponins. So, these fractions are attractive candidate for the isolation and identification of effective ingredients.
Apoptosis-inducing proteins with reduced expression in breast cancer: A review article
Mohammad Mehdi Khaleghi, Faezeh Rouhi, Kourosh Eslami, Fatemeh Shafiee
Biochemistry and Biophysics Reports, 2025
In vitro Evaluation of the Cytotoxic Effects of a Recombinant form of the Soluble Mutant IL-6 Receptor on an Ovarian Cancer Cell Line
Iranian Journal of Biotechnology, 2025
DNA fragmentation factor 40-based therapeutic approaches for cancer: a review article
Faezeh Masaeli, Saba Omoomi, Fatemeh Shafiee
Medical Oncology, 2024
Kinetic studies, molecular docking, and antioxidant activity of novel 1,3-diphenyl pyrazole-thiosemicarbazone with anti-tyrosinase and anti-melanogenesis properties
Fateme Azimi, Mohammad Mahdavi, Mehdi Khoshneviszadeh, Fatemeh Shafiee, Mahin Azimi, et al.
Bioorganic Chemistry, 2024
Optimization of the Production of Soluble Recombinant TEV Protease in Two E. coli Strains
Matineh Shahriari, Fatemeh Shafiee, Fatemeh Moazen, Hamid Mir Mohammad Sadeghi
Avicenna Journal of Medical Biotechnology, 2024
Intein Based Fusion Proteins: Great Tags for the Soluble Production and Convenient Purification of Recombinant Proteins
Fatemeh Shafiee, S. Sharifi, Armin Amini
Iranian Journal of Biotechnology, 2024
Recombinant production of interleukin-1 receptor antagonist in fusion to albumin binding domain with potential affinity to human serum albumin
Fatemeh Shafiee, Ali Yazdani
Research in Pharmaceutical Sciences, 2024
Production of recombinant DNA fragmentation factor 40 in fusion to an antimicrobial peptide from spider venom and evaluation of its cytotoxic effects
Zahra Shafiee-Ardestani, Fatemeh Shafiee
Research in Pharmaceutical Sciences, 2024
Recombinant Production of TP4-LYC1, A New Chimeric Peptide with Targeted Cytotoxicity to HeLa Cells
Hanieh Mohammad Pour, Ali Jahanian-Najafabadi, Fatemeh Shafiee
Avicenna Journal of Medical Biotechnology, 2024
An investigation to find the correlation between lupus anticoagulant and coagulation abnormalities in COVID-19 patients; a narrative review
Amir Aria, Ahmadreza Maghsoudi, Fatemeh Shafiee, Mahnaz Momenzadeh
Journal of Nephropathology, 2024
The Antimicrobial Effects of TP4-LYC1 Fusion Protein against Multidrug-Resistant A. Baumannii
Journal of Isfahan Medical School, 2023
Preparation and in Vitro Evaluation of Inhalation Powder Containing Fluconazole Nanoparticles for the Treatment of Fungal Lung Infections
Journal of Isfahan Medical School, 2023
Design and construction of scFv-PE35KDEL as a novel immunotoxin against human epidermal growth factor receptor 2 for cancer therapy
Zahra Shariaty Vaziri, Fatemeh Shafiee, Vajihe Akbari
Biotechnology Letters, 2023
Arsenic Polyoxotungstate-Zeolitic Imidazolate Framework-8 as a Potential Selective Anti-cancer Nano Platform
Zahra Moazeni Bistgani, Fatemeh Shafiee, Jaleh Varshosaz, Hossein Kazemian, Shahram Tangestaninejad, et al.
Journal of Inorganic and Organometallic Polymers and Materials, 2023
Development and evaluation of wafer loaded with sertaconazole solid dispersion for the treatment of oral candidiasis
Horieh Hajhashemi, Somayeh Taymouri, Fatemeh Shafiee
Brazilian Journal of Pharmaceutical Sciences, 2023
Andrographolide protects against doxorubicin-and arsenic trioxide-induced toxicity in cardiomyocytes
Leila Safaeian, Fatemeh Shafiee, Samira Haghighatnazar
Molecular Biology Reports, 2023
Invasive fungal infections in hematologic malignancies: Incidence, management, and antifungal therapy
Journal of Research in Medical Sciences, 2023

Fatemeh Shafiee

RESEARCH INTERESTS

Scopus Publications