Gamal Ali Abdelhafez
Verified @azhar.edu.eg
Department of Animal production, Faculty of Agriculture, Al-Azhar University, Nasr City, Cairo, Egypt
Professor
RESEARCH INTERESTS
Poultry Nutrition
3
Scopus Publications
Scopus Publications
- A rapid and simple single-step method for the purification of Toxoplasma gondii tachyzoites and bradyzoites
Saeed El‐Ashram, Yu Zhang, Yongsheng Ji, Dina Salama, Kun Mei, Li Zhili, Huang Shujian, Haoji Zhang, Shawky M. Aboelhadid, Reem A. Alajmi,et al.
Wiley
AbstractThis study describes a simple method for the large‐scale isolation of pure Toxoplasma gondii tachyzoites and bradyzoites. T. gondii tachyzoites were obtained from infected human foreskin fibroblasts (HFFs) and peritoneal exudates of mice, while tissue cysts containing bradyzoites were collected from chronically infected mice. Harvested cells and brain tissues were incubated in Hanks balanced salt solution (HBSS), containing 0.25% trypsin and 0.5% taurodeoxycholic acid (TDC) for 5 min. Subsequent washes in phosphate buffered saline (PBS) were conducted, and the cell viability of the preparations was good, as determined by flow cytometry and ability to reinfect HFF cells and propagate in mice. The purification procedure allowed for a rapid preparation of pure T. gondii tachyzoites and bradyzoites in sufficient quantity that can be used for downstream procedures. The advantage of the new method is that it is convenient and inexpensive. - Effects of nanochitosan supplementation on productive performance of Japanese quail
Saeed El-Ashram, Gamal Ali Abdelhafez, and Khaled Yehia Farroh
Elsevier BV
Summary The present study evaluated the effects of chitosan nanoparticles (ChNP) as a feed additive during the 1-42 d of age on growth performance, blood serum parameters, carcass traits, intestinal bacteria, digestive enzymes, and fat digestibility. Two hundred and forty of 1-d-old Japanese quail were randomly distributed into four groups and fed a basal diet for 42 d. Groups 1, 2, 3, and 4 were fed 0.0 mg/kg diet (control group), 200-mg ordinary chitosan/kg diet, 50-mg ChNP/kg diet, and 200-mg ChNP/kg diet, respectively. The highest (P - Effects of phytogenic supplementation on productive performance of broiler chickens
Saeed El-Ashram and Gamal A. Abdelhafez
Elsevier BV
Summary The present study evaluated the feed additive containing essential oils of thyme and star anise from 1 to 42 days of age on growth performance, physiological parameters, carcass traits, intestinal bacterial community, antioxidant status of meat and economic efficiency in broilers under Egyptian weather condition. Two hundred and seventy one-day-old Indian River (IR) broiler chicks were randomly distributed in three groups and fed a basal diet for 42 days. The control (Co) group, T1 group, and T2 group were fed 0.0 mg/kg diet, 150 mg/kg diet and 300 mg/kg diet, respectively. The highest final weight and weight gain were recorded by the T2 and T1 groups compared to the control group (p