miRBiT: a rules-based single-sample serum miRNA classifier for pan-cancer detection with multi-cohort validation Pandikannan Krishnamoorthy, Madhavan Parthasarathy, Nilanjana Das, Athira S Raj, Ganakalyan Behera, Ashok Kumar, Vikas Gupta, Saikat Das, Himanshu Kumar Briefings in Bioinformatics, 2026 Liquid biopsy offers a minimally invasive approach to early cancer diagnosis. MicroRNAs (miRNAs) are small, non-coding RNAs showing excellent diagnostic potential due to their stability and dysregulation upon different physiological conditions. However, existing miRNA-based cancer classifiers rely on cohort-based comparisons, limiting their clinical utility. Extensive analyses in this study present miRNA binary trend (miRBiT), a miRNA rules-based single-sample classifier, trained on 16,190 samples, tested across nine independent datasets, and further validated on 8 distinct disease cohorts. miRBiT utilizes miRNA expression signatures at an intra-sample level to classify ‘cancer’ and ‘non_cancer’ samples, including healthy and other diseases with high sensitivity and specificity, enabling personalized predictions. Additionally, an interactive web application, miRBiT Explorer serum miRNA expression resource, is developed to visualize and validate serum miRNA expression patterns in 46,349 samples. This study highlights the potential of miRNAs in robust cancer classification, enabling personalized, minimally invasive cancer screening and early detection at an unprecedented scale.
Rhino sinusitis caused by Lasiodiplodia theobromae in a diabetic patient Anand Kumar Maurya, Sweta Kumari, Ganakalyan Behera, Arati Bhadade, Karuna Tadepalli Medical Mycology Case Reports, 2023 We report an uncommon instance of rhinosinusitis by Lasiodiplodia theobromae in a known diabetic patient. A melanized fungus called Lasiodiplodia theobromae causes a typical plant disease that rots fruits and plants. Infections in humans are currently limited. Mostly from tropical and subtropical regions, there have been few reported occurrences. The fungus has been associated with clinical manifestations such as onychomycosis, corneal ulcers, and phaeohyphomycosis. Identification by phenotype was inconclusive. DNA sequencing was used for final identification. Amphotericin B and surgical debridement were effective treatments for the patient.
Recurrent eccrine acrospiroma of the parotid region: A case report Vikas Gupta, Utkal Priyadarshi Mishra, Ganakalyan Behera Journal of the Nepal Medical Association, 2021 Eccrine acrospiroma is a benign tumor of skin and adnexa arising from eccrine sweat gland epithelium. It is usually solitary, slow growing tumor commonly affecting extremities. Rarely it affects head and neck region, and extremely rare in parotid region. Females are affected more often. Treatment of choice is wide local excision with adequate skin margins. Although benign this tumor is very notorious for recurrence after inadequate resection. We describe here a case of young male patient with recurrent eccrine acrospiroma over parotid region which was managed by wide local excision with primary repair with excellent results.
Multicenter epidemiologic study of coronavirus disease-associated mucormycosis, India Atul Patel, Ritesh Agarwal, Shivaprakash M. Rudramurthy, Manoj Shevkani, Immaculata Xess, Ratna Sharma, Jayanthi Savio, Nandini Sethuraman, Surabhi Madan, Prakash Shastri, Deepak Thangaraju, Rungmei Marak, Karuna Tadepalli, Pratik Savaj, Ayesha Sunavala, Neha Gupta, Tanu Singhal, Valliappan Muthu, Arunaloke Chakrabarti, and Emerging Infectious Diseases, 2021 During September-December 2020, we conducted a multicenter retrospective study across India to evaluate epidemiology and outcomes among cases of coronavirus disease (COVID-19)-associated mucormycosis (CAM). Among 287 mucormycosis patients, 187 (65.2%) had CAM; CAM prevalence was 0.27% among hospitalized COVID-19 patients. We noted a 2.1-fold rise in mucormycosis during the study period compared with September-December 2019. Uncontrolled diabetes mellitus was the most common underlying disease among CAM and non-CAM patients. COVID-19 was the only underlying disease in 32.6% of CAM patients. COVID-19-related hypoxemia and improper glucocorticoid use independently were associated with CAM. The mucormycosis case-fatality rate at 12 weeks was 45.7% but was similar for CAM and non-CAM patients. Age, rhino-orbital-cerebral involvement, and intensive care unit admission were associated with increased mortality rates; sequential antifungal drug treatment improved mucormycosis survival. The COVID-19 pandemic has led to increases in mucormycosis in India, partly from inappropriate glucocorticoid use.
Extended Procedures Pankuri Mittal, Hitesh Verma, Amit Kesari, R. S. Virk, Kshitiz Charya, Smriti Panda, Alok Thakar, Rajesh Kumar Meena, Ramesh S. Doddamani, Manish Gupta, Rohit Verma, Vikas Gupta, Ganakalyan Behera, Amit Shanker, Namrita Mahmi, M. Ravi Sankar, Arulalan Mathialagan Essentials of Rhinology, 2021
Medullary thyroid carcinoma co-existent with Hashimoto's thyroiditis diagnosed by a comprehensive cytological approach Sheetal Malpani, Ashwani Tandon, Hemlata Panwar, Ujjawal Khurana, Neelkamal Kapoor, Ganakalyan Behera, Vikas Gupta Diagnostic Cytopathology, 2020 Fine‐needle aspiration cytology (FNAC) is a preliminary test for the diagnosis of thyroid lesions. We hereby report a rare case of medullary thyroid carcinoma (MTC) co‐existing with Hashimoto's thyroiditis (HT). This case was substantiated with ancillary tests on cytology material to give a novel insight. A 60‐year‐old female presented clinically with diffuse enlargement of the thyroid, and right‐side nodule on ultrasonography. FNAC of the isthmic area showed features of HT, while cytology of right‐side nodule displayed sheets of plasmacytoid cells and frequent scattered large bizarre and pleomorphic cells, lymphoglandular bodies and pale eosinophilic material. The differential diagnosis of MTC with co‐existent HT or high‐grade lymphoma was considered. To establish the diagnosis, serum calcitonin and ancillary studies on aspirated material were carried out. High serum calcitonin (7251 pg/mL), Congophilia on smears, and CD 45−ve; CD56+ve expression of tumor cells on flowcytometric analysis established the diagnosis of MTC over lymphoma. This diagnosis was further confirmed upon histopathology. Ancillary studies on aspirated material established the diagnosis of MTC and excluded the diagnosis of lymphoma. Establishing the correct diagnosis was cardinal in such a scenario as these diseases have extremely diverse management.
Tracheal amyloidosis presenting as life threatening airway obstruction Alkesh Kuma Khurana, Vikas Gupta, Abhishek Goyal, Ganakalyan Behera, Garima Goel Journal of Clinical and Diagnostic Research, 2017 We hereby present a case of a 62-year-old man who presented with tracheostomy in situ for the last two years and unexplained etiology for the life-threatening airway compromise. Thorough work up revealed tracheal amyloidosis as the cause for airway obstruction. This case describes not only the uncommon presentation of the disease but also the isolated nature of deposition of amyloid.
Isolated Lichen Planus of Lower Lip: A Case Report Dillip Kumar Samal, Ganakalyan Behera, Vikas Gupta, Kaushik Majumdar, Ujjawal Khurana Indian Journal of Otolaryngology and Head and Neck Surgery, 2014
