Host metabolomic responses in recurrent P. vivax malaria Michael N. Yakubu, Victor I. Mwangi, Rebeca L. A. Netto, Maria G. C. Alecrim, Jessica R. S. Alves, et al. Scientific Reports, 2024 Malaria is the leading parasitic disease worldwide, with P. vivax being a major challenge for its control. Several studies have indicated metabolomics as a promising tool for combating the disease. The study evaluated plasma metabolomic profiles of patients with recurrent and non-recurrent P. vivax malaria in the Brazilian Amazon. Metabolites extracted from the plasma of P. vivax-infected patients were subjected to LC–MS analysis. Untargeted metabolomics was applied to investigate the metabolic profile of the plasma in the two groups. Overall, 51 recurrent and 59 non-recurrent patients were included in the study. Longitudinal metabolomic analysis revealed 52 and 37 significant metabolite features from the recurrent and non-recurrent participants, respectively. Recurrence was associated with disturbances in eicosanoid metabolism. Comparison between groups suggest alterations in vitamin B6 (pyridoxine) metabolism, tyrosine metabolism, 3-oxo-10-octadecatrienoate β-oxidation, and alkaloid biosynthesis II. Integrative network analysis revealed enrichment of other metabolic pathways for the recurrent phenotype, including the butanoate metabolism, aspartate and asparagine metabolism, and N-glycan biosynthesis. The metabolites and metabolic pathways predicted in our study suggest potential biomarkers of recurrence and provide insights into targets for antimalarial development against P. vivax.
Anthocyanin Profiles in the Tropical Fruits Eugenia jambolana and Inga edulis: A Comparative Study Using Paper Spray Ionization (PSI-MS), Tissue Spray Ionization (TSI-MS), and Direct Infusion (DI-MS) Gesiane S. Lima, Nerilson M. Lima, Naará S. Balbino, Jean C. P. Sousa, Gabriel F. Santos, et al. Chemistry and Biodiversity, 2024 Paper spray ionization (PSI‐MS) and tissue spray ionization (TSI‐MS) mass spectrometry are simple and rapid ambient ionization mass spectrometry techniques that offer numerous advantages over conventional analysis methods. This study aims to analyze the efficiency of detecting anthocyanins from Eugenia jambolana fruit peel and Inga edulis seeds using PSI‐MS, TSI‐MS, and DI‐MS (direct infusion). DI‐MS exhibited high efficiency, detecting all compounds in abundance, with anthocyanins malvidin 3,5‐O‐diglucoside (1) and petunidin 3,5‐O‐diglucoside (2) being the most prevalent. PSI‐MS, however, struggled to detect delphinidin 3‐O‐glucoside and showed lower abundances for compounds 1, 2, 3 (delphinidin 3,5‐O‐diglucoside), and 4 (petunidin 3‐O‐glucoside) compared to DI‐MS, attributed to the technique's challenges with molecular weight and polarity. TSI‐MS was least effective, detecting only compounds 1, 2, and 3 at low intensities. The overall unique compounds identified across techniques were 134, emphasizing the importance of comprehensively employing multiple methods to analyze anthocyanins in these edible plants.
Infraspecific Chemical Variability and Metabolomic Profiling by Paper Spray Ionization (PSI-MS) of Averrhoa carambola from Different Brazilian Biomes Naará S. Balbino, Nerilson M. Lima, Hugo G. Machado, Gesiane S. Lima, Jean C. P. Sousa, et al. Chemistry and Biodiversity, 2024 This research focused on the molecular diversity of A. carambola collected from three Brazilian biomes (Cerrado, Amazônia, and Mata Atlântica), whose results revealed significant differences in metabolite profiles among these biomes through PSI‐MS analysis. Chemometric analysis provided valuable insights into the clustering patterns and metabolic distinctions. Cerrado and Mata Atlântica biomes exhibited a 70 % similarity, indicating a notable degree of resemblance. In Cerrado, carambolaside A was notably abundant, while carambolaside M was low in Amazônia and moderate in Cerrado samples. Carambolaside B was abundant in Amazônia but relatively low in the Cerrado and Mata Atlântica. In contrast, the Amazônia biome samples appeared to be more dissimilar. In Cerrado, epicatechin, kaempferol, and procyanidin B showed lower abundance, while apigenin, quercetin, myricetin, and rutin displayed moderate levels. Mata Atlântica showed relatively higher levels of kaempferol, quercetin, and rutin. This study indicated the environmental influence on secondary metabolites production in A. carambola fruits.
Endophytic fungal community associated with Colombian plants Hernando José Bolívar-Anillo, Ezzanad Abdellah, Gesiane da Silva Lima, Inmaculada Izquierdo-Bueno, Javier Moraga, et al. Neotropical Endophytic Fungi Diversity Ecology and Biotechnological Applications, 2021
Chemical composition and acaricidal activity of essential oils and selected terpenes from two species of psidium in the cerrado biome of brazil against Tetranychus urticae Boletin Latinoamericano Y Del Caribe De Plantas Medicinales Y Aromaticas, 2020
