Gisele Silvestre da Silva
@embrapa.br
Embrapa Tropical Agroindustry
RESEARCH INTERESTS
Agrochemistry
Scopus Publications
Scopus Publications
Priscilla Alves, Jhonyson Guedes, Luiz Serrano, Marlon Martins, Debora de Sousa, Gisele Silva, Paulo Ribeiro, Dávila Zampieri, and Guilherme Zocolo
Sociedade Brasileira de Quimica (SBQ)
Investigating specialized plant metabolites, traditionally referred to as ‘secondary metabolites’ present in leaf extracts of cashew trees (Anacardium occidentale) resistant and susceptible to anthracnose disease was carried out using metabolomics combined with chemometric tools. We used clones of dwarf-cashew with the following variations of characteristics: resistant and healthy (CCP 76, BRS 226, BRS 189), susceptible and healthy (BRS 265), and another clone also susceptible but affected by the disease (BRS 265). The UPLC-QTOF-MSE (ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry) analysis of the leaves of Anacardium clones allowed us annotation a total of 39 metabolites. The multiple orthogonal partial discriminant analysis of the least-squares (OPLS-DA) allowed twelve metabolites to be potential biomarkers of differentiation among the clones studied. Namely, the triene-(17:3)-anacardic acid found on CCP 76 and BRS 189 clones, respectively, is the main candidate for biomarker of resistance. While catechin, B-type procyanidin isomers, and procyanidin dimer monogallate identified in BRS 265 are significant potential biomarkers of susceptibility.
Ana K. F. Leite, Thatyane V. Fonteles, Thaiz B.A.R. Miguel, Giselle Silvestre da Silva, Edy Sousa de Brito, Elenilson G. Alves Filho, Fabiano A.N. Fernandes, and Sueli Rodrigues
Elsevier BV
This study evaluated the atmospheric cold plasma (ACP) effect on cashew apple juice composition at different frequencies (200 and 700 Hz). The impact of this non-thermal technology on the organic juice compounds after the processing and along with the in vitro digestion carried out in a simulated digestion system at 37 °C/6 h was evaluated. The changes in the juice composition were determined by NMR spectroscopy and chemometric analyses. Vitamin C and total phenolic compounds were also quantified in processed and non-processed (control) juices and after each digestion phase. The results showed decreased glucose and fructose in samples treated by ACP and an increment in malic acid concentration for ACP700. ACP increased the amount of vitamin C in the juices and did not affect the total phenolic content. The gastric digestion highlighted the pronounced effect of plasma on the juice composition, increasing all of the components detected by NMR. Cashew apple juice processed by ACP700 presented a higher concentration of malic acid and phenylalanine. An increased bioaccessibility of vitamin C was also found for ACP700. Although ACP processing has decreased some compounds' concentration, this technology improved the bioaccessibility of vitamin C - the main bioactive compound of cashew apple juice.
Marcelo Oliveira Holanda, Sandra Machado Lira, José Ytalo Gomes da Silva, Chayane Gomes Marques, Lia Corrêa Coelho, Carla Laíne Silva Lima, Joana Talita Galdino Costa, Gisele Silvestre da Silva, Glauber Batista Moreira Santos, Guilherme Julião Zocolo,et al.
Elsevier BV
Ana Carolina Viana de Lima, Ana Paula Dionisio, Fernando Antonio Pinto de Abreu, Gisele Silvestre da Silva, Rodolfo Dantas Lima Junior, Hilton César Rodrigues Magalhães, Deborah dos Santos Garruti, Idila Maria da Silva Araújo, Adriana Guirado Artur, Carlos Alberto Kenji Taniguchi,et al.
Elsevier BV
Complete characterization of microfiltered red-purple pitaya colorant (MRPPC) and its potential applications in foods is described. Using sensorial analysis, products that use carmine or beetroot dye as a food colorant in their formulations were compared. The effect of storage under refrigeration on the microbiological, physicochemical, and chemical changes of MRPPC were evaluated. The results showed that UPLC-ESI-QTOF-MSE was effective for the simultaneous determination of twenty metabolites, putatively identified as carbohydrates, flavonoids, and betalains. The MRPPC was shown to have microbiological and physicochemical stability through twelve weeks of storage, and chemometric analyses efficiently distinguished the metabolic profile in each storage period. Sensory analysis revealed that the MRPPC was useful as a food colorant in yogurt, where it improved color quality without affecting aroma and other characteristics. These results indicate that MRPPC is promising food ingredient as a natural red-purple colorant.
Aurilene Vasconcelos, Elenilson Alves Filho, Tigressa Rodrigues, Adriely Vieira, Renato Innecco, Gisele Silva, and Guilherme Zocolo
Sociedade Brasileira de Quimica (SBQ)
The collection time could be an important target for optimizing technological processes in obtaining essential oils of interest to agribusiness. The objective of this study was to distinguish the time for the collecting of Achillea millefolium L. leaves, which provides the highest yield and quality of the essential oil. Also, identify the type of polyethylene packaging and leaf storage period during the one-year period that would maintain the essential oil characteristics. Gas chromatography-mass spectrometry and chemometric studies were performed into two steps trial to detect changes in chemical profile induced by different conditions. First, an analysis of the leaf collection time was performed using chromatographic data from six different gathering times throughout the day. After determining the best time to collect from the leaves, the essential oil was extracted in five storage periods over a year. The highest oil content was observed in leaves harvested between 11 and 15 h, with a maximum of 39 min after 13 h. Therefore, it is recommended to perform extraction in the early afternoon. There was no significant statistical differentiation related to polyethylene packages. In addition, it is recommended that the essential oil can be stored without significant changes for up to six months.
Caio Castro, Licia Luz, Jhonyson Guedes, Diogo Porto, Maria Francilene Silva, Gisele Silva, Paulo Ribeiro, Kirley Canuto, Edy Brito, Dávila Zampieri,et al.
Sociedade Brasileira de Quimica (SBQ)
Myracrodruon urundeuva (“aroeira-do-sertão”) is a species threatened with extinction due to anthropogenic exploitation. Phytochemical analysis of bark, branch and leaf extracts revealed the presence of several compounds such as flavonoids, phenols, tannins, quercetin derivatives and anacardic acids. Dereplication methodology was performed to tentatively identify 50 compounds analyzed by ultra-performance liquid chromatography coupled with an electrospray ionization quadrupole time-of-flight mass spectrometry operating in MS mode (UPLC-QTOF-MS). The extracts exhibited anti-tumor effect in cancer cells HCT-116 (colorectal), SF-295 (glioblastoma), HL-60 (leukemia), and RAJI (leukemia). Also, these results correlate with the principal component analysis (PCA) data that identified three distinct groups indicating, efficiently, metabolic differences between organs of M. urundeuva. Through discriminatory analysis of the orthogonal partial least squares (OPLS-DA), the variable of importance in the projection (VIP) and S-Plot, we were able to determine 30 potential biomarkers. The fingerprint of hydroethanolic extracts was correlated with the cytotoxicity assay and demonstrated a significant difference in the composition of plant extract.
Akenaton O.C.V. Gomes, Maria V. Brito, Ricardo A. Marques, Leandro B. Lima, Igor M. Cavalcante, Tellin D.N. Vieira, Fátima M. Nunes, Mary A.S. Lima, Daniel E. Uchôa, Cristiano S. Lima,et al.
Elsevier BV
Abstract Microorganisms can produce a great variety of enzymes in short growing time, and their enzyme complexes have been used in multi-step bioconversions of natural products. Whole cells from different fungal strains were capable of biotransforming diterpenes through a variety of reactions, most of them involving enzymes that promote carbon oxidations. In this paper, we report the first biotransformation of the cytotoxic pimarane-type diterpene annonalide (1) into nor-annonalide (2) by growing cells of the filamentous fungus Fusarium oxysporum f. sp. tracheiphilum (UFCM 0089). Lipase, decarboxylase and alcohol dehydrogenase (ADH) enzymes mediated this unprecedented bioconversion. Theoretical studies (DFT analysis, molecular docking and molecular dynamics) were performed as an attempt to propose a plausive pathway involved on the formation of product 2, and binding interactions of intermediate 1a into the catalytic site of pyruvate decarboxylase enzyme were identified.
Jhonyson Guedes, Elenilson Alves Filho, Maria Silva, Tigressa Rodrigues, Christiane Ramires, Maria Lima, Gisele Silva, Cláudia Pessoa, Kirley Canuto, Edy Brito,et al.
Sociedade Brasileira de Quimica (SBQ)
Agroindustrial residues, such as leaves of fruit plants, can be sources of bioactive molecules, thus adding value to co-products that are rarely explored in agroindustry. In this context, this study aimed to look into the phytochemical profiles in detail and to explore the antitumor potential of S. tuberosa and S. mombin leaves. We observed that, S. tuberosa leaf extract was cytotoxic in both tumor and healthy cells. S. mombin extracts selectively inhibited cell proliferation in the tumor cell line for prostate cancer (PC3) and did not significantly affect healthy cells. The metabolic profiles of the extracts were evaluated by gas chromatography coupled with mass spectrometry and twenty-three metabolites were identified. The correlation of metabolic profiles with cytotoxic tests indicated possible chemical markers that may be responsible for the inhibition of cell proliferation. This study revealed that the unexplored co-products in agroindustry may have great therapeutic potential, and therefore should be screened for biologically active compounds
Glauber Batista Moreira Santos, Ana Paula Dionísio, Hilton César Rodrigues Magalhães, Fernando Antonio Pinto de Abreu, Sandra Machado Lira, Ana Carolina Viana de Lima, Gisele Silvestre da Silva, Jhonyson Arruda Carvalho Guedes, Idila Maria da Silva Araujo, Adriana Guirado Artur,et al.
Elsevier BV
The effects of processing on the chemical, physicochemical, enzymatic, and volatile metabolic composition of pitaya pulp were assessed for the first time. To this end, the following treatments to obtain pitaya pulp were evaluated: Treatment A (TA, pulp processing without ascorbic acid), Treatment B (TB, whole fruit processing with ascorbic acid), and Control (whole fruit processing without ascorbic acid). The treatment employed in TB resulted in low polyphenol oxidase and peroxidase activity, and no significant chemical or physicochemical alterations in most parameters evaluated. In addition, TB presents high yields and fiber content compared to the TA or Control. For metabolic analysis, Gas Chromatography-Mass Spectrometry (GC-MS) was effective for the simultaneous determination of 80 volatile metabolites in pitaya. Chemometric analyses was used to efficiently distinguish the volatile compounds of each treatment, and demonstrated that TB presents an interesting volatile profile due the conservation or agregation of compounds.
Sandra Machado Lira, Ana Paula Dionísio, Marcelo Oliveira Holanda, Chayane Gomes Marques, Gisele Silvestre da Silva, Lia Coêlho Correa, Glauber Batista Moreira Santos, Fernando Antonio Pinto de Abreu, Francisco Ernani Alves Magalhães, Emanuela de Lima Rebouças,et al.
Elsevier BV
Pitaya is a Cactacea with potential for economic exploitation, due to its high commercial value and its functional components - such betalains, oligosaccharides and phenolic compounds. Although the biological activities of pitaya have been studied using in vivo and in vitro models (anti-inflammatory and antiproliferative activities, as example), its anxiolytic-like effect is still unexplored. Therefore, the aim of this work was to perform a characterization of pulp and peel of pitaya (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) using UPLC-QTOF-MSE, and to assess its toxicity and anxiolytic-like effect in adult zebrafish (Danio rerio). The results showed 16 and 15 compounds (in pulp and peel, respectively), including maltotriose, quercetin-3-O-hexoside, and betalains, putatively identified by UPLC-QTOF-MSE. Thus, pitaya pulp and peel showed no toxicity in both models tested (Vero cell lines and zebrafish model, LC50 ˃ 1 mg/mL); and a significant anxiolytic activity, since the treated fish reduced the permanence in the clear zone (Light & Dark Test) compared to that in the control, exhibiting anxiolytic-simile effect of diazepam. However, these effects were reduced by pre-treatment with the flumazenil suggesting that the pulp and peel of pitaya are anxiolytics agents mediated via the GABAergic system. These findings suggested that H. polyrizhus has the potential of developing an alternative plant-derived anxiolytic therapy. In addition, pitaya peel (which is a waste in the food industry) should be regarded as a valuable product, which has the potential as an economic value-added ingredient for anxiety disorders.