Glauco Botelho dos Santos

@uff.br

Departamento de Odontologia Restauradora
Universidade Federal Fluminense

EDUCATION

Graduação em Odontologia pela Faculdade de Odontologia de Nova Friburgo (1997), é especialista em Dentística pela USP (1999) e Implantodontia (2015), mestre em Odontologia (Materiais Dentários) pela Universidade de São Paulo (2003) e doutor em Odontologia (Dentística) pela UERJ (2008)

RESEARCH INTERESTS

COMPÓSITOS, CERÂMICAS, FOTOATIVAÇÃO

FUTURE PROJECTS

Desenvolvimento de materiais e de técnicas restauradoras adesivas objetivando a melhoria no desempenho e na longevidade dos procedimentos restauradores

Os objetivos específicos deste projeto são: 1) Caracterizar um sistema adesivo experimental de dois frascos, contendo diferentes concentrações (1%, 3% e 5%) de silicato de sódio e silicato de magnésio visando avaliar propriedades de selamento, resistência mecânica, biocompatibilidade e efeitos antimicrobianos. 2) Avaliar a efetividade de técnicas de tratamento da dentina visando melhorias na adesividade e na diminuição da permeabilidade dentinária/ sensibilidade pós-operatória..


Applications Invited
23

Scopus Publications

Scopus Publications

  • Effects of magnification on restorative dental preparation performance: a scoping review and level of evidence mapping
    Fernando Alexandria Soares de Oliveira, Vittorio Moraschini, Daniel Costa Ferreira de Almeida, and Gustavo Oliveira dos Santos

    Springer Science and Business Media LLC

  • Accuracy of artificial intelligence in implant dentistry: A scoping review with systematic evidence mapping
    Vittorio Moraschini, Daniel Costa Ferreira de Almeida, Rafael Seabra Louro, Alice Maria de Oliveira Silva, Mario Pereira Couto Neto, Gustavo Oliveira dos Santos, and José Mauro Granjeiro

    Elsevier BV

  • EVALUATION OF RECEMENTATION PROTOCOLS FOR DISLODGED GLASS FIBER POSTS
    Ísis Ingrid Nogueira Simões, E. M. da Silva, A. G. Penelas, L. Poskus, G. B. dos Santos and José Guilherme Antunes Guimarães

    Quintessence Publishing
    PURPOSE To evaluate different protocols for recementation of dislodged glass fiber posts (GFPs). MATERIALS AND METHODS The root canals of 50 bovine incisors were prepared to receive GFPs, which were conditioned with 24% hydrogen peroxide, silanized, and cemented with a self-adhesive resin cement. After light curing, the specimens were submitted to a pullout test. The dislodged GPFs were distributed into five experimental groups (n = 10 each) according to surface treatment for recementation: NT = no treatment; SI = silane; ET = 92.8% ethanol/silane; HP = 24% hydrogen peroxide/silane; AL = airborne-particle abrasion (50-μm alumina)/silane. The GFPs were recemented with the same self-adhesive resin cement, and specimens were submitted to a second pullout test. Data were analyzed by Student t test and one-way ANOVA (α = .05). RESULTS For all groups, the recementation bond strength was higher than the strength obtained after the first cementation (P < .05). There were no statistical differences among the experimental recementation protocols (P = .096). CONCLUSION All recementation protocols might be reliably applied since they led to higher bond strength than the first cementation.

  • Protocols for Mechanical Cleaning of the Post Space on the Bond Strength Between Root Dentin and Cementation System
    CM Alencar, JRE Verbicário dos Santos, FFA Jassé, GO dos Santos, WG Escalante-Otárola, GM Castro-Nuñez, EA de Campos, and MC Kuga

    Operative Dentistry
    SUMMARY Objective To evaluate the effects of mechanical versus chemical cleaning protocols for cleaning the root dentin surface before cementation of fiberglass posts for their effect on the bond strength, failure mode, and dentinal penetration of the cementing agent using an etch-and-rinse adhesive system on dentin prepared to receive a fiberglass post. Methods Forty roots of bovine teeth were endodontically treated and prepared for fiber post cementation. The specimens were randomized into 4 groups of 10: Control group (CO) - irrigation with 2.5% NaOCl; DW group- irrigation with distilled water; RB group - rotating brush for cleaning root canals, and CUI group - continuous ultrasonic irrigation. The fiberglass posts were cemented, and the specimens were immersed in distilled water for 6 months. A push-out test was performed on the cervical, middle, and apical thirds of the samples. Dentinal penetration of the cementing agent and the fracture pattern were evaluated by laser confocal microscopy. Statistical analysis was performed using analysis of variance (ANOVA) and post hoc Tukey tests (α=0.05). Results: The RB and CUI groups showed significantly higher bond strength values when compared to the Control and DW groups (p&amp;lt;0.05). In addition, in the control and DW groups, the apical third presented lower bond strength values when compared to middle and cervical thirds. Conclusion While DW showed the highest incidence of adhesive type failure, CUI resulted in the highest dentinal penetration of the cementing agent (p&amp;lt;0.05). RB and CUI resulted in the highest bond strength between cementation system and root dentin. In addition, CUI favored greater dentinal penetration of fiberglass post cementing agent.

  • Evaluation of Surgical Gown Textiles for Resistance Against Aerosols: A Preclinical Double-Blind Study
    Eliane Porto Barboza, Gustavo Oliveira dos Santos, Caroline Montez, Luiza Abreu Sendra, Esio de Oliveira Vieira, Waldimir Carvalho, and Vinicius Farias Ferreira

    Frontiers Media SA
    Personal protective equipment is used to prevent healthcare workers from contaminants. With the advent of COVID-19 pandemic, the use of effective PPE becomes more critical to avoiding infection by SARS-CoV-2. This preclinical double-blind study evaluated the performance of surgical gown textiles for penetration resistance against aerosols. The different textiles were divided into seven groups: (1) SMS PP OE Phobic; (2) Laminated Phobic; (3) Medical Barrier; (4) Trilayer Fabric; (5) Impermeable gown; (6) Impermeable with heat sealing tape; and (7) Clean Wear. All groups were exposed to aerosol-generating procedure for 5 min, according to Barboza et al. 2020. All measurements were recorded and exported to a datasheet for analysis using SPSS software. The groups showed significant differences (p &amp;lt; 0.01). Groups 2, 3, 4, 5, and 6 proved to be effective barriers against aerosols and presented a constant behavior. Groups 1 and 7 showed pigmented areas of 13.05 and 48.23%, respectively, suggesting that, in the present test model, these water-repellent and antimicrobial fabrics were not effective barriers against 5-min aerosols generated by a high-speed dental handpiece. Polyethylene or polyurethane laminated fabrics were efficient against 5-min aerosols. The SMS textile, globally used for surgical gowns, and the cotton fabric, impregnated with nanoparticles, were not effective barriers. The breathability and comfort of these textiles should be tested in future studies. Healthcare workers should be aware of the exact specifications of their surgical gown textiles.

  • Are Non-woven Gowns Safe for Dental Professionals? A Preclinical Double-Blind Study
    Eliane Porto Barboza, Gustavo Oliveira dos Santos, Caroline Montez, Luiza Abreu Sendra, Esio Oliveira Vieira, and Vinicius Farias Ferreira

    Frontiers Media SA
    With the advent of the coronavirus disease 2019 (COVID-19) pandemic, the risk of dental professional's infection increases exponentially due to aerosol-generating procedures. The protective performance of dental professional gown fabrics is a major challenge against microorganisms and aerosols. Globally, health care workers wear gowns manufactured with non-woven fabrics. However, these professionals may have a false greater sense of protection based on the greater weight of the gown's fabric. This study evaluated whether aerosol generated by dental procedures has the potential to pass through three different non-woven weights (40, 60, and 80 g/m2). Single and folded formats of each weight were analyzed. The positions of the mannequin and the high-speed handpiece were standardized. The non-woven samples were placed over a sheet of sulfite paper and fixed on a marked position of a standardized smooth surface, 10 cm away from the end of the mannequin's head, simulating the dental professional body. For better visualization, the water used in the equipment was tinted with water-soluble red artificial dye. Blind to the nature of the groups, one researcher started the 5 uninterrupted min of aerosol production. The sheets were randomized coded, so that the samples could not be identified by the evaluator. Another researcher, blind to the tests, scanned all sheets and the images were analyzed by ImageJ software. The groups showed significant differences (p < 0.01). Except for the folded 80 g/m2, all groups presented pigmented mean areas ranging from 16.18% (60 g/m2 folded) to 35.68% (40 g/m2 single), suggesting that the one-layer non-woven samples were not effective as barriers for 5 uninterrupted min of aerosol spray. Dental procedures exceeding the studied time may provide different results with the non-woven 80 g/m2 folded. Dental health workers, who are exposed daily to aerosol-generating procedures, should be aware of the exact specification and performance of the fabrics used in the manufacturing of their disposable garments. If the use of an impermeable surgical gown is not possible during aerosol-generating procedures, a plastic garment associated with a disposable gown should be worn, as recommended by the World Health Organization.

  • Cryotherapy in reducing pain, trismus, and facial swelling after third-molar surgery: Systematic review and meta-analysis of randomized clinical trials
    Edmundo Marques do Nascimento-Júnior, Gustavo Marques Sobral dos Santos, Mário Luis Tavares Mendes, Maximiliano Cenci, Marcos Britto Correa, Tatiana Pereira-Cenci, and Paulo Ricardo Saquete Martins-Filho

    Elsevier BV

  • Effect of different times of nonthermal argon plasma treatment on the microtensile bond strength of self-adhesive resin cement to yttria-stabilized tetragonal zirconia polycrystal ceramic
    Alexandre Barboza Elias, Renata Antoun Simão, Maíra Prado, Paulo Francisco Cesar, Glauco Botelho dos Santos, and Eduardo Moreira da Silva

    Elsevier BV
    Statement of problem. Nonthermal argon plasma may increase the surface energy of yttria‐stabilized tetragonal zirconia polycrystal (Y‐TZP) dental ceramics. However, studies that evaluated the effect of increased plasma treatment times on the bond strength of resin cements to Y‐TZP ceramics are lacking. Purpose. The purpose of this in vitro study was to evaluate the effect of different nonthermal argon plasma (NTAP) treatment times on the surface energy and bond strength of a self‐adhesive resin cement to Y‐TZP ceramic. Material and methods. Forty‐eighty Y‐TZP plates were divided into 2 groups (n=24): as‐sintered (AS) and airborne‐particle abrasion (APA) with 50‐&mgr;m Al2O3, which were subdivided into 4 groups (n=6) according to the time of NTAP treatment: 0, 20, 60, and 120 seconds. The surface energy was evaluated with a goniometer. Forty Y‐TZP blocks submitted to the same surface treatments (8 groups; n=5) were cemented to composite resin blocks, using a self‐adhesive resin cement. After storage in distilled water at 37°C for 24 hours, the Y‐TZP‐composite resin blocks were cut into beams and submitted to a microtensile bond strength (&mgr;TBS) test. Data were analyzed using 2‐way ANOVA and the Tukey honestly significant differences test (&agr;=.05). Results. Treatment with NTAP increased the surface energy for AS and APA groups (P<.05). For both groups, the &mgr;TBS was as follows: 0 seconds < 20 seconds < 60 seconds = 120 seconds (P<.05). Only after 120 seconds of NTAP treatment was the &mgr;TBS of APA higher than that of AS (P<.05). Conclusions. Treatment with NTAP improved the surface energy and increased the &mgr;TBS of self‐adhesive resin cement to Y‐TZP ceramic, with higher times of plasma treatment resulting in higher bond strength.

  • Impact of bisphosphonate therapy on dental implant outcomes: An overview of systematic review evidence
    V. Mendes, G.O. dos Santos, M.D. Calasans-Maia, J.M. Granjeiro, and V. Moraschini

    Elsevier BV
    The purpose of this overview was to assess the methods, quality, and outcomes of systematic reviews conducted to evaluate the impact of bisphosphonates on dental implants and the risk of developing bisphosphonate-related osteonecrosis of the jaw after dental implant surgery. An electronic search without date or language restriction was performed in the PubMed/MEDLINE, Cochrane CENTRAL, Web of Science, and LILACS databases (to January 2018). Eligibility criteria included systematic reviews that evaluated the impact of bisphosphonates on implant outcomes. The quality assessment of the included reviews was done using AMSTAR 2 guidelines. The protocol of this overview was registered in PROSPERO (CRD42018089617). The search and selection process yielded seven reviews, published between 2009 and 2017. None of the systematic reviews included in this study obtained the maximum score in the quality assessment. The scientific evidence available demonstrates that patients with a history of bisphosphonate use do not present a higher risk of dental implant failure or marginal bone loss compared to patients who have not used bisphosphonates. The literature also suggests that patients who undergo surgical trauma during the installation of dental implants may be more susceptible to bisphosphonate-related osteonecrosis of the jaw.


  • Effectiveness for dentin hypersensitivity treatment of non-carious cervical lesions: a meta-analysis
    Vittorio Moraschini, Larissa Salina da Costa, and Gustavo Oliveira dos Santos

    Springer Science and Business Media LLC
    ObjectiveThe aim of this review is to conduct a systematic review and meta-analysis comparing the effectiveness of in-home or in-office treatments for dentin hypersensitivity.Materials and methodsAn electronic search without restriction on dates or languages was performed in four electronic databases until March 2017. In addition, hand-searches in regular journals and in the gray literature were also conducted. To develop the search strategy, clinical questions were formulated using the PICOS method. Eligibility criteria included randomized clinical trials (RCTs) that compared the effectiveness of different agents for the treatment of dentin hypersensitivity through chemical occlusion, physical occlusion, nerve desensitization, or photobiomodulation (low-level light therapy). This systematic review was registered in PROSPERO under number CRD42016039394.ResultsTwenty-five RCTs (16 parallel; 9 split-mouth), published from 1992 to 2016, were included. The results of the meta-analysis showed that in-office subgroups treated with chemical or physical occlusion of dentin tubules and nerve desensitization had a statistically significant difference from placebo, with P < 0.00001, P < 0.00001, and P = 0.02, respectively. For in-home treatments, the results of the meta-analysis showed that only those subgroups treated with chemical occlusion of dentin tubules and nerve desensitization exhibited a statistically significant difference from placebo, with P < 0.00001 and P = 0.03, respectively.ConclusionsThe results of pairwise meta-analysis suggest that among in-office treatments, dentinal tubule occlusion (whether chemical or physical) and nerve desensitization provide the best outcomes for treatment of dentin hypersensitivity. For in-home treatments, only chemical occlusion of dentin tubules and nerve desensitization showed a greater treatment efficacy than placebo and the difference was statistically significant.

  • Interrelation of periodontal parameters between asthmatics and nonasthmatics subjects: a systematic review and meta-analysis
    Vivian Mendes, Gustavo Oliveira dos Santos, and Vittorio Moraschini

    Elsevier BV
    BACKGROUND The aim of this systematic review (SR) is to evaluate the association between asthma and periodontal parameters. METHODS An electronic search without date or language restrictions ​​was carried out in MEDLINE, Cochrane, Web of Science, and LILACS until May 2017. In addition, manual search and in the grey literature were also conducted. The search process, data analysis, and quality assessment were performed by two independent reviewing authors. Eligibility criteria included prospective and retrospective cohort studies, case-controls, and randomized clinical trials. For the meta-analysis, the inverse variance method was used in fixed or random effect models, which were chosen according to heterogeneity. The estimates of the intervention effects were expressed as the mean differences. RESULTS The search and selection process yielded 21 studies, published between 1979 and 2017. The meta-analysis showed a statistically significant difference for the parameters of gingival bleeding, plaque index, and gingival index for asthmatic participants with P<0.0001, P<0.0001, and P=0.0005, respectively. CONCLUSIONS The data from this SR suggest that asthmatic patients may be more susceptible to negative periodontal changes, althought further high-quality research wuold be welcome.

  • Noninvasive approach to esthetic rehabilitation of conoid anterior teeth


  • Effect of intra-oral aging on t→m phase transformation, microstructure, and mechanical properties of Y-TZP dental ceramics
    Luciana Meireles Miragaya, Renato Bastos Guimarães, Rodrigo Othávio de Assunção e Souza, Glauco dos Santos Botelho, José Guilherme Antunes Guimarães, and Eduardo Moreira da Silva

    Elsevier BV
    The aim of the present study was to evaluate the influence of intra-oral aging on the tetragonal-to-monoclinic (t→m) phase transformation of two Y-TZP dental ceramics - Lava Frame (Frame) and Lava Plus (Plus) - and determine the impact of this response on their microstructures and mechanical properties: flexural strength, Young's modulus, microhardness and fracture toughness. Standardized ceramic specimens were analyzed by X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). After the baseline analysis, the specimens were attached to personalized intra-oral resin appliances and exposed to the oral cavity of 20 subjects for 60 days and then analyzed again. Specimens produced for mechanical properties evaluation were also analyzed before and after the 60-day intra-oral aging. The data were analyzed using two-way ANOVA and Tukey HSD's post hoc test (α=0.05). Weibull analysis was used to evaluate the strength reliability. Both Y-TZP ceramics suffered t→m phase transformation after 60-day intra-oral aging (Plus=4.7%/Frame=7.7%). SEM and AFM analyses showed dislodgement of ZrO2 grains and a significant increase in roughness after intra-oral aging for both ceramics. Both Y-TZP ceramics suffered a decrease on flexural strength, Young's modulus and fracture toughness after intra-oral aging (p<0.05). Only Plus presented a decrease of microhardness after intra-oral aging (p<0.05). Intra-oral aging for 60 days produced t→m phase transformation, changed the microstructure and decreased the mechanical properties of two Y-TZP dental ceramics.

  • ZnCl<inf>2</inf> Incorporated into Experimental Adhesives: Selected Physicochemical Properties and Resin-Dentin Bonding Stability
    Giselle Soares Almeida, Eduardo Moreira da Silva, José Guilherme Antunes Guimarães, Rayssa Nogueira Lamego da Silva, Glauco Botelho dos Santos, and Laiza Tatiana Poskus

    Hindawi Limited
    The aim of this study was to evaluate the degree of conversion (DC%), water sorption (WS), solubility (SO), and resin-dentin bonding stability of experimental adhesive systems containing ZnCl2. Different concentrations (wt.%) of ZnCl2 were added to a model etch-and-rinse adhesive system consisting of BISGMA, HEMA, UDMA, GDMA, water, and ethanol: Zn0 (0%-control group); Zn2 (2%); Zn3.5 (3.5%); and Zn5 (5%). Adper Single Bond 2 (SB) was used as commercial reference. The samples were light cured for 20s using a quartz-tungsten-halogen unit (650 mW/cm2). DC% (n=5) was measured using FT-IR spectroscopy, and WS and SO (n=5) were calculated based on ISO4049. Microtensile bond strength (μTBS) and nanoleakage (NL) were measured after 24 h and 12 months of water storage (n=10). Data were analyzed using ANOVA and Tukey’s HSD test (5%). Zn5 presented the lowest DC% and the highest WS and SO (p&lt;0.05). Zn0 and Zn2 presented statistically similar DC%, WS, SO, and immediate μTBS. All adhesives containing ZnCl2 maintained a μTBS stability after 12 months, but only Zn2 and Zn3.5 did not suffer an increase in NL. SB presented the highest immediate μTBS but the greatest reduction after 12 months (p&lt;0.05). The addition of 2 wt.% of ZnCl2 in adhesive formulations seems to be a promising way to improve the resin-dentin bonding stability. Higher concentrations than 2 wt.% could impair some physicochemical properties.

  • Characterization of conventional and high-translucency Y-TZP dental ceramics submitted to air abrasion
    Bhenya Ottoni Tostes, Renato Bastos Guimarães, Jaime Dutra Noronha-Filho, Glauco dos Santos Botelho, José Guilherme Antunes Guimarães, and Eduardo Moreira da Silva

    FapUNIFESP (SciELO)
    Abstract This study evaluated the effect of air-abrasion on t®m phase transformation, roughness, topography and the elemental composition of three Y-TZP (Yttria-stabilized tetragonal zirconia polycrystal) dental ceramics: two conventional (Lava Frame and IPS ZirCad) and one with high-translucency (Lava Plus). Plates obtained from sintered blocks of each ceramic were divided into four groups: AS (as-sintered); 30 (air-abrasion with 30 mm Si-coated Al2O3 particles); 50 (air-abrasion with 50 mm Al2O3 particles) and 150 (air-abrasion with 150 mm Al2O3 particles). After the treatments, the plates were submitted to X-ray diffractometry; 3-D profilometry and SEM/EDS. The AS surfaces were composed of Zr and t phases. All treatments produced t®m phase transformation in the ceramics. The diameter of air-abrasion particles influenced the roughness (150&gt;50&gt;30&gt;AS) and the topography. SEM analysis showed that the three treatments produced groove-shaped microretentions on the ceramic surfaces, which increased with the diameter of air-abrasion particles. EDS showed a decrease in Zr content along with the emergence of O and Al elements after air-abrasion. Presence of Si was also detected on the plates air-abraded with 30 mm Si-coated Al2O3 particles. It was concluded that irrespective of the type and diameter of the particles, air-abrasion produced t®m phase transformation, increased the roughness and changed the elemental composition of the three Y-TZP dental ceramics. Lava Plus also behaved similarly to the conventional Y-TZP ceramics, indicating that this high translucency ceramic could be more suitable to build monolithic ceramic restorations in the aesthetic restorative dentistry field.

  • Resin-dentin bond stability of experimental 4-META-based etch-and-rinse adhesives solvated by ethanol or acetone
    C. Amaral, Alice Marques Diniz, Eugênio Braz Rodrigues Arantes, Glauco Botelho dos SANTOS, J. D. Noronha-Filho and E. D. da Silva


    PURPOSE To investigate the influence of 4-META concentration and type of solvent on the degree of conversion (DC%) and resin-dentin bond stability of experimental etch-and-rinse adhesives. MATERIALS AND METHODS Four different concentrations of 4-META (12 wt%, 20 wt%, 30 wt%, 40 wt%) were added to a model adhesive system consisting of TEG-DMA (25 wt%), UDMA (20 wt%), HEMA (30 wt%), water (4 wt%), camphorquinone (0.5 wt%), and tertiary amine (0.5 wt%) dissolved in 20% acetone (A12, A20, A30 and A40) or 20% ethanol (E12, E20, E30 and E40). DC% was evaluated by FT-IR spectroscopy. Human molars were wet ground until the occlusal dentin was exposed, the adhesive systems were applied after 37% phosphoric acid etching, and resin composite buildups were incrementally constructed. After storage in distilled water at 37°C for 24 h, the teeth were cut into resin-dentin beams (cross-sectional area 1 mm2). Microtensile bond strength (μTBS) was evaluated after 24 h, 6 months, and 1 year of water storage at 37°C. The failure mode was categorized as adhesive, mixed, or cohesive. Data were analyzed using ANOVA and Tukey's HSD test (α = 0.05). RESULTS A12 presented the lowest DC% (p < 0.05). All the other adhesive systems showed statistically similar DC% (p > 0.05). All adhesive systems maintained resin-dentin bond stability after 6 months of water storage, while only A40 and E40 maintained it after 1 year. CONCLUSION Irrespective of the type of organic solvent, the incorporation of high concentrations of 4-META (40 wt%) improved the resin-dentin bond stability of the experimental etch-and-rinse adhesive systems over a period of 1 year.

  • Effect of HEMA phosphate as an alternative to phosphoric acid for dentin treatment prior to hybridization with etch-and-rinse adhesive systems
    T. Foscaldo, Glauco Botelho dos SANTOS, L. Miragaya, M. Garcia, V. Hass and E. D. da Silva


    PURPOSE To evaluate the effect of dentin treatment using HEMA phosphate (HEMA-P) on the microtensile bond strength (μTBS) and nanoleakage of an etch-and-rinse adhesive system. MATERIALS AND METHODS The occlusal surfaces of human molars were wet ground until superficial dentin was exposed. The specimens were then assigned to two groups according to dentin treatment: PA: 37% H₃PO₄ for 15 s; or HP: HEMA-P for 15 s. Adper Single Bond 2 was applied to the treated dentin surfaces and resin composite buildups were incrementally constructed over them. After 24-h storage in artificial saliva at 37°C, the bonded teeth were cut into resin-dentin sticks with a cross-sectional area of 1 mm², which were submitted to μTBS testing immediately or after 3 months of storage in artificial saliva at 37°C. Nanoleakage was assessed using SEM/EDS, and the interaction between dentin and H₃PO₄ or HEMA-P was evaluated by combining micro-Raman and FT-IR spectroscopy. The data were analyzed using two-way ANOVA and Tukey's HSD post-hoc test (α = 0.05). RESULTS HP presented significantly higher μTBS than PA at both times (p < 0.05). Both treatments maintained μTBS stability after 3 months of artificial saliva storage (p > 0.005). At both times, PA presented higher nanoleakage than HP (p < 0.05). CONCLUSIONS Both dentin treatments maintained μTBS stability after 3 months of artificial saliva storage. The use of HEMA-P was associated with less nanoleakage than was traditional phosphoric-acid etching.

  • Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability
    Eduardo Moreira da Silva, Carolina Ullmann Fernandes de Sá Rodrigues, Marcos Paulo de Oliveira Matos, Thais Rodrigues de Carvalho, Glauco Botelho dos Santos, and Cristiane Mariote Amaral

    Elsevier BV
    OBJECTIVES To evaluate the degree of conversion (DC%), water sorption (WS), solubility (SO) and the resin-dentin bonding stability (μTBS) of experimental (EXP) etch-and-rinse adhesive systems containing MMP-inhibitors: Galardin-GAL, Batimastat-BAT, GM1489-GM1 and chlorhexidine diacetate-CHX. METHODS DC% was measured using FT-IR spectroscopy, while WS and SO were calculated based on ISO4049. Thirty-six human molars were wet ground until the occlusal dentin was exposed. The adhesive systems were applied and resin composite buildups were incrementally constructed. After 24 h immersion in distilled water at 37 °C, the specimens were cut into resin-dentin beams with a cross-sectional area of 1 mm(2). The μTBS was evaluated after 24 h, 6 months and 12 months of water storage at 37 °C. Adper Single Bond 2 (SB2) was used as a commercial control. The data were analyzed using ANOVA and Tukey's HSD test. RESULTS SB2 presented the highest DC% (p<0.05). CHX presented the lowest WS (p<0.05). GM1, GAL and BAT presented similar WS when compared with SB2 (p>0.05). SO was found to be not significant (p>0.05). All adhesive systems maintained μTBS stability after 6 months of water storage. Only BAT, GM1 and CHX maintained μTBs stability after 12 months of water storage. CONCLUSIONS The experimental adhesive systems with GM1489 and chlorhexidine diacetate presented the best physicochemical properties and preserved resin-dentin bonding stability after 12 months of water storage. CLINICAL SIGNIFICANCE GM1489 could be suitable for inclusion as an MMP-inhibitor in etch-and-rinse adhesive systems to maintain resin-dentin bonding stability over time.

  • Amalgam and resin composite longevity of posterior restorations: A systematic review and meta-analysis
    Vittorio Moraschini, Cheung Ka Fai, Raphael Monte Alto, and Gustavo Oliveira dos Santos

    Elsevier BV
    OBJECTIVES The aim of the present review was to evaluate by means of a systematic review and meta-analysis the hypothesis of no difference in failure rates between amalgam and composite resin posterior restorations. DATA Randomized controlled trials, controlled clinical trials and prospective and retrospective cohort studies were included in this review. The eligibility criteria included clinical trials in humans with at least 12 months of follow-up comparing the failures rates between occlusal and occlusoproximal amalgam and composite resin restorations. Clinical questions were formulated and organized according to the PICOS strategy. SOURCE An electronic search without restriction on the dates or languages was performed in PubMed/MEDLINE, Cochrane Central Register of Controlled Trials, and Web of Science up until March 2015. STUDY SELECTION The initial search resulted in 938 articles from PubMed/MEDLINE, 89 titles from the Cochrane Central Register of Controlled Trials, and 172 from the Web of Science. After an initial assessment and careful reading, 8 studies published between 1992 and 2013 were included in this review. According to the risk of bias evaluation, all studies were classified as high quality. CONCLUSIONS The results of this review suggest that composite resin restorations in posterior teeth still have less longevity and a higher number of secondary caries when compared to amalgam restorations. In relation to fractures, there was no statistically significant difference between the two restorative materials regarding the time of follow-up. CLINICAL SIGNIFICANCE There is currently a worldwide trend towards replacing amalgam restorations with mercury-free materials, which are adhesive and promote aesthetics. It is important to perform an updated periodic review to synthesize the clinical performance of restorations in the long-term.

  • Light transmission on dental resin composites
    G.B. dos Santos, R.V. Monte Alto, H.R. Sampaio Filho, E.M. da Silva, and C.E. Fellows

    Elsevier BV
    OBJECTIVES The purposes of this study was: (1) to examine the light transmittance characteristics of two light-cured resin composites, for different thickness, (2) to correlate the light transmittance through the resin composites and the filler contents, and (3) to determine the penetration depth of the light as a function of the wavelength. METHODS Two resin composites (Filtek Z250, shade A2 and Filtek Supreme XT, shade A2E) were used. Specimens of six different thicknesses (0.15, 0.25, 0.30, 0.36, 0.47 and 0.75 mm) were prepared (n=3). The transmittance at wavelengths from 400 to 800 nm was measured using a UV-visible spectrophotometer, before and after light polymerization. RESULTS AND SIGNIFICANCE Significant differences were found in the wavelength dependence of transmittance between the two materials, and between the unpolymerized and polymerized stages of each resin composite. At lower wavelengths, the light transmittance of the Filtek Supreme XT resin composite was lower than the Filtek Z250. At the higher wavelengths, however, Filtek Supreme XT presented higher light transmittance. For both resin composites, the penetration depth was higher after polymerization. However, Filtek Supreme XT showed a higher gain in transmittance at the 0.15 mm thickness. The difference in light transmittance characteristics of the resin composites may affect their depth of polymerization.

  • The influence of C-factor, flexural modulus and viscous flow on gap formation in resin composite restorations
    E. Moreira da Silva, G. O. dos Santos, J. G. A. Guimarães, A. A. L. Barcellos, and E. M. Sampaio

    Operative Dentistry
    Clinical Relevance Resin composites with high viscous flow and low flexural modulus may reduce gap formation in resin composite restorations.

  • Analysis of gap formation at tooth-composite resin interface: Effect of C-factor and lightcuring protocol
    Gustavo Oliveira dos Santos, Antônio Henrique Monteiro da Fonseca Thomé da Silva, José Guilherme Antunes Guimarães, Alexandre de Araújo Lima Barcellos, Eduardo Martins Sampaio, and Eduardo Moreira da Silva

    FapUNIFESP (SciELO)
    Objective: The aim of this study was to evaluate the effect of C-factor and light-curing protocol on gap formation in composite resin restorations. Material and Methods: Cylindrical cavities with 5.0 mm diameter and three different depths (A=1.0, B=2.0 and C=3.0 mm) were prepared on the occlusal surface of 30 human molars and restored in a single increment with P 60. The composite resin was light-cured according to two protocols: standard - 850 mW/cm2 / 20 s and gradual - 100 up to 1000 mW/cm2/ 10 s + 1000 mW/cm2 / 10 s. After storage in distilled water (37°C/7 days), the restorations were cut into three slices in a buccolingual direction and the gap widths were analyzed using a 3D-scanning system. The data were submitted to ANOVA and Student-Newman-Keuls test (α=0.05). Results: ANOVA detected a significant influence for the C-factor and light-curing protocol as independent factors, and for the double interaction C-factor vs. light-curing protocol. Cavities with higher C-factor presented the highest gap formation. The gradual light-curing protocol led to smaller gap formation at cavity interfaces. Conclusions: The findings of this study suggest that the C-factor played an essential role in gap formation. The gradual light-curing protocol may allow relaxation of composite resin restoration during polymerization reaction.

RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)

Light transmission on dental resin composites
CEF G B dos Santos,R V Monte Alto,H R Sampaio Filho, E M da Silva
Dental Materials 24 (5), 571-576 127* 2008

Composite depth of cure obtained with QTH and LED units assessed by microhardness and micro-Raman spectroscopy
GB Santos, IS Medeiros, CE Fellows, A Muench, RR Braga
Operative dentistry 32 (1), 79-83 92 2007

Experimental etch-and-rinse adhesive systems containing MMP-inhibitors: Physicochemical characterization and resin-dentin bonding stability
EM da Silva, CUF de Sá Rodrigues, MP de Oliveira Matos, ...
Journal of dentistry 43 (12), 1491-1497 21 2015

Effect of intra-oral aging on t→ m phase transformation, microstructure, and mechanical properties of Y-TZP dental ceramics
LM Miragaya, RB Guimarães, ROA e Souza, G dos Santos Botelho, ...
Journal of the Mechanical Behavior of Biomedical Materials 72, 14-21 16 2017

Resin-dentin bond stability of experimental 4-META-based etch-and-rinse adhesives solvated by ethanol or acetone
CM Amaral, AM Diniz, EB Arantes, GB Dos Santos, JD Noronha-Filho, ...
J Adhes Dent 18 (6), 513-520 11 2016

Effect of different times of nonthermal argon plasma treatment on the microtensile bond strength of self-adhesive resin cement to yttria-stabilized tetragonal zirconia …
AB Elias, RA Simão, M Prado, PF Cesar, GB dos Santos, EM da Silva
The Journal of prosthetic dentistry 121 (3), 485-491 8 2019

ZnCl2 incorporated into experimental adhesives: selected physicochemical properties and resin-dentin bonding stability
GS Almeida, EM da Silva, JGA Guimarães, RNL da Silva, GB Dos Santos, ...
BioMed Research International 2017 4 2017

Effect of HEMA Phosphate as an Alternative to Phosphoric Acid for Dentin Treatment Prior to Hybridization with Etchand-Rinse Adhesive Systems.
T Foscaldo, GB Dos Santos, LM Miragaya, M Garcia, V Hass, EM da Silva
Journal of Adhesive Dentistry 18 (5) 4 2016

Characterization of conventional and high-translucency Y-TZP dental ceramics submitted to air abrasion
BO Tostes, RB Guimarães, JD Noronha-Filho, GS Botelho, ...
Brazilian Dental Journal 28 (1), 97-104 3 2017

Uso de sistema de fibras de reforço em prótese adesiva posterior: relato de caso clínico
RV Monte-Alto, GO Santos, GB Santos, KRHC Dias, MS Miranda
Rev. dental press estét, 102-114 2 2009

Influence of brushing on enamel surface morphology submitted to a bleaching gel.
JH Correa, VM Valadao, GB Santos, RVM Alto, EM Silva
JOURNAL OF DENTAL RESEARCH 82, 185-185 2 2003

Resin-dentin bond stability of etch-and-rinse adhesive systems with different concentrations of MMP inhibitor GM1489
MESNG MIRANDA, EM SILVA, MF OLIVEIRA, FS Simmer, GB SANTOS, ...
Journal of Applied Oral Science 28 2020

Influência do Protocolo de Fotoativação na Cimentação de Pinos de Fibra de Vidro
LC Pegado, AG Penelas, GB Santos, JGA Guimarães
Journal of Health Sciences 19 (5), 58-58 2017

Restauração de dentes tratados endodonticamente com pino de fibra de vidro e acessório em canais amplos
RVM Alto, GB Santos, RSMS Lima, LT Poskus, EM Silva, MS Miranda
Clín. int. j. braz. dent, 60-68 2009

Influence of a light-curing protocol on the polymerization depth of a restorative composite material.
LMN Pimentel, RVM Alto, RC Coviello, GB Santos, EM Silva
JOURNAL OF DENTAL RESEARCH 82, 184-184 2003

Evaluation of a softstart technique of a light-curing unit.
GB Santos, RVM Alto, CF Santos, JGA Guimaraes, EM Silva, A Muench
JOURNAL OF DENTAL RESEARCH 82, 280-280 2003

Effect of storage in water on dentin bonding using two adhesive systems.
CCG Nogueira, GB Santos, RC Coviello, JGA Guimaraes, EM Silva
JOURNAL OF DENTAL RESEARCH 82, 183-183 2003

Influence of two dentinal treatments following contamination with blood and/or saliva on bond strength to bovine dentin.
RC Coviello, LMN Pimentel, GB Santos, EM Silva, JGA Guimaraes
JOURNAL OF DENTAL RESEARCH 82, 183-183 2003

Evaluation of two alternative methods for application of two adhesive systems.
CF Santos, RVM Alto, GB Santos, AAL Barcellos, EM Silva
JOURNAL OF DENTAL RESEARCH 82, 183-183 2003

Estudo da microdureza Knoop e do grau de conversão de um compósito em função da profundidade e fontes ativadoras
GB Santos
2003

The soft start polimerization effect on shear bond strength of two-dentin bonding agents
AB Vasconcellos, EM Silva, AAL Barcellos, GB Santos, RVM Alto, ...
JOURNAL OF DENTAL RESEARCH 81, B128-B128 2002

SoftStart polymerization influence in microleakage of resin composite restorations.
PA Lopez, EM Silva, RP Mattos, RO Campany, GB Santos, LMN Pimentel
JOURNAL OF DENTAL RESEARCH 81, B127-B127 2002

Cimento de ionomero de vidro
GB Santos
1999

Dicas: Pinos de Fibra de Vidro Personalizados
RV Monte-Alto, GB dos Santos, GO dos Santos, J Noronha