Agricultural and Biological Sciences, Genetics, Molecular Biology, Plant Science
43
Scopus Publications
796
Scholar Citations
17
Scholar h-index
28
Scholar i10-index
Scopus Publications
Induction of plant resistance to bean common mosaic virus (BCMV) via multiple applications of nanoparticle-conjugated dsRNA Nulevino Iralu, Sumiah Wani, Shahjahan Rashid, Farhana Wani, Dasari Meghanath, Gowhar Ali, Aflaq Hamid BMC Plant Biology, 2026 Bean common mosaic virus (BCMV) is regarded as the most destructive to common beans, capable of causing yield losses of up to 100%. A non-transgenic approach involving the topical application of dsRNA to induce RNA interference is increasingly being utilized to develop efficient, eco-friendly, sustainable virus and vector management strategies. This approach does not require genetic modification and is particularly beneficial for crops that are challenging to modify through transgenesis or gene editing. This study aimed to evaluate the efficacy of multiple applications of nanoparticle-conjugated dsRNA in three different treatment assemblages, applied at varying time intervals, for the effective management of BCMV. Results from qRT-PCR assay showed significant downregulation of viral expression in all three assemblages with lower expression values upto 50 dpi, confirming the prolonged efficacy of topically applied dsRNA inducing RNAi. Following three topical applications of nanoparticle-conjugated dsRNA, viral expression in treated plants was significantly reduced to 1.218 and 1.493 for dsRNA targeting the HC-Pro and CP genes, respectively, as compared to a high viral expression value of 22.994 in positive control. This research represents a significant advancement in BCMV management, demonstrating that multiple applications of dsRNA can effectively suppress viral infection till the flowering stage of the crop and can be utilised as an environmental-benign and non-toxic alternative to manage viral infections in plants.
Genomics-informed elucidation of trait-phenotype relationships and MABB approaches deliver major gene blast resistance in the aromatic rice landrace Mushk Budji Asif Bashir Shikari, Raheel Shafeeq Khan, Noor-ul Ain, F. A. Mohiddin, Gazala Hassan Khan, Najeeb-ul-Rehman Sofi, Zahoor A. Dar, M. Ashraf Ahangar, Gowhar Ali, Aflaq Hamid Wani, Bilal A. Padder Frontiers in Genetics, 2026 Introduction A novel set of pyramided lines for durable blast resistance was developed using marker-assisted backcross breeding (MABB) strategy in the genetic background of the aromatic landrace Mushk Budji (MB). Methods Simultaneous-but-stepwise transfer of the blast resistance genes Pi54 and Pi9 was achieved through the integration of foreground selection (FS) and background genome recovery processes, aided by genome-wide SSR and >1500 KASP markers. MABB, whole genome re-sequencing, coupled with stringent phenotypic selection for aroma, amylose content, kernel dimensions, and cooking quality, helped minimize the linkage drag and achieve early recurrent parent genome (RPG) recovery in the inter-cross BC 2 F 2:3 generation. Results The two-gene lines carrying Pi9 + Pi54 were developed through inter-crossing corresponding near-isogenic lines (NILs) with an RPG of approximately 90%. With the help of sequencing of the derived NILs, we were able, for the first time, to establish the role of major alleles underlying rice quality and stress resilience in MB. In the process, we confirmed the retention of favorable alleles at key genetic loci, such as BADH2 (aroma), Wx (amylose content), Rc (white pericarp), Hd1/Hd4/Hd5 (heading date), and COLD1/COLD6 (cold tolerance) in the derived NILs. GGE biplot analysis revealed stable performance of five advanced lines across target ecologies. Discussion The set of NILs was useful in elucidating the phenotypic effects of 11 genes related to grain type, quality, and adaptability traits in the landrace MB. Multi-environment screening for blast resistance, at hot spot locations, in addition to artificial inoculation, validated the resistance response of the lines to both leaf and neck blast. This study demonstrates the successful integration of genomics-assisted breeding and phenotypic selection to improve a heritage rice variety for enhanced disease resistance and ecological adaptation. The development of improved MB lines represents a rare endeavor towards the area expansion and conservation of the heirloom rice.
DUS Characterization of Rajmash (Phaseolus vulgare L.) Genotypes for Morpho-physiological Traits under Rainfed Conditions Khusboo Tariq, Gowhar Ali, Zahoor A Dar, Asif Shikari, Aflaq Hamid, Ajaz A Lone, Aman Tutlani, Nusrat ul Islam, Andleeb Javaid, Rajneesh Kumar Legume Research, 2025 Background: The current investigation was carried out in the year 2021-22 at Dryland Agriculture Research Station (DARS), Budgam. The experiment was laid out in randomized complete block design with three replications in field and completely randomized design (CRD) in vitro conditions for evaluating different traits of genotypes. DUS characterization was conducted as per the DUS testing guidelines by Protection of Plant Varieties and Farmers Act, Authority, Descriptors for rajmash (Phaseolus vulgaris L.) GOI, New Delhi [reproduced from Plant Variety Journal of India. Vol.1 (1), 2007]. Methods: The observations were recorded on 18 DUS characters including morphological and quality attributes and physiological characters. For all the features studied, the analysis of variance for morpho-physiological and qualitative attributes found significant differences, providing adequate chance to select the genotypes with desired qualities. DUS traits were examined in accordance with the descriptor, “state” and “code” provided for each character. Result: The observed range of variation were recorded for different traits for different genotypes. Cluster analysis for morphological, maturity, yield and quality characteristics arranged the rajmash genotypes into 11 clusters, as per Mahalanobis D2 analysis using Tocher’s method with cluster III having the maximum number of genotypes (8), followed by cluster I, II, IV and V with 6 genotypes, 2 genotypes were grouped in cluster VII and XI and the remaining clusters with 1 genotype each. Diversity analysis showed grouping of genotypes in 11 clusters, which will help in selection of genetically divergent parents for exploitation in hybridization programme and would constitute an essential gene pool for future breeding programme.
Recent Advances in Crop Variety Identification Using DNA Technology Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Preface Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Integration of DNA Technology with Conventional Approaches for Crop Varietal Identification Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Multi-locus Profiling and Estimation of Genetic Diversity Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Varietal Identification in Pulse Crops Using Molecular Markers: Current Status and Prospects Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Bioinformatics Tools for Crop Variety Identification Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Role of DNA Technology in Seed Purity Testing Recent Advances in Crop Variety Identification Using DNA Technology, 2025
PCR-Based Markers in Crop Variety Identification Recent Advances in Crop Variety Identification Using DNA Technology, 2025
DNA Fingerprinting in Rapeseed Mustard Varieties and Germplasm: Recent Status and Future Prospects Recent Advances in Crop Variety Identification Using DNA Technology, 2025
DNA Fingerprinting of Wheat Varieties and Germplasm: Present Status and Future Prospects Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Statistical Methods for Analyzing Genetic Data in Crop Variety Identification Recent Advances in Crop Variety Identification Using DNA Technology, 2025
Variability studies in maize genotypes under temperate conditions of Kashmir, India Plant Archives, 2014
Impact of corm weight on saffron yield under temperate conditions of Kashmir Vegetos, 2012
Demography of saffron growing villages of Kashmir Asif M. Iqbal, Sheikh Sameer Samad, F.A. Nehvi, Aijaz A. John, Ameekue Arshid, Aijaz A. Lone, Gowhar Ali, Salvee Yasmeen, Jyoti Sethi Medicinal Plants, 2012
RECENT SCHOLAR PUBLICATIONS
Genetic Divergence and Population Structure in Sprouting Broccoli (Brassica oleracea var. italica Plenck) Revealed through Cluster and Principal Component Analyses A Fayaz, RA Dar, A Amin, B Afroza, R Rashid, G Ali, UH Masoodi, ... Journal of Advances in Biology & Biotechnology 29 (4), 482-490 , 2026 2026
Induction of plant resistance to bean common mosaic virus (BCMV) via multiple applications of nanoparticle-conjugated dsRNA N Iralu, S Wani, S Rashid, F Wani, D Meghanath, G Ali, A Hamid BMC Plant Biology , 2026 2026
Genomics-informed elucidation of trait-phenotype relationships and MABB approaches deliver major gene blast resistance in the aromatic rice landrace Mushk Budji AB Shikari, RS Khan, N Ain, FA Mohiddin, GH Khan, NR Sofi, ZA Dar, ... Frontiers in Genetics 16, 1699333 , 2026 2026 Citations: 1
Genomics-informed elucidation of trait-phenotype relationships and MABB approaches deliver major gene blast resistance in the aromatic rice landrace Mushk Budji AB Shikari, RS Khan, N Ain, FA Mohiddin, GH Khan, NR Sofi, ZA Dar, ... Frontiers in Genetics 16, 1699333 , 2026 2026
Correlation and Path Coefficient Analysis in Sprouting Broccoli (Brassica oleracea var. italica Plenck): An Experimental Research RA Dar, A Fayaz, B Afroza, G Ali, N Nazir, AM Aezum, G Gani, JA Baba, ... Journal of Scientific Research and Reports 31 (11), 833-840 , 2025 2025
Correlation and Path Analysis for Yield and Its Contributing Traits in Summer Squash (Cucurbita pepo L.) U Maqbool, A Amin, B Afroza, G Ali, N Nazir, PA Sheikh, M Rashid Journal of Experimental Agriculture International 47 (10), 341-349 , 2025 2025
Assessment of Genetic Variability, Heritability and Expected Genetic Advance in Summer Squash (Cucurbita pepo L.) U Maqbool, A Amin, B Afroza, G Ali, N Nazir, PA Sheikh, I Abidi, G Nazir, ... International Journal of Plant & Soil Science 37 (10), 143-154 , 2025 2025
Identification and Molecular Validation of Bean Common Mosaic Virus Resistant Donors in Common Bean ( Phaseolus vulgaris L.) R Farooq, R Rashid, B Afroza, ARM Al Tawaha, A Karnwal, A Hamid, ... Indian Journal of Microbiology 65 (3), 1546-1553 , 2025 2025 Citations: 1
Establishing an efficient protoplast isolation and PEG-mediated transformation system for functional genomic studies in Colletotrichum lindemuthianum A Nabi, Y Raunta, RA Shah, I Lateef, Z Rashid, N Nabi, T Gowhar, ... Physiological and Molecular Plant Pathology 138, 102677 , 2025 2025
Delineating the source of resistance to bean common mosaic virus (BCMV) and bean common mosaic necrosis virus (BCMNV) in common bean (Phaseolus vulgaris) cultivars of Jammu and … D Meghanath, S Wani, S Bashir, S Rashid, A Javaid, ZA Dar, SH Wani, ... Frontiers in microbiology 16, 1614122 , 2025 2025 Citations: 2
Topical application of cocktail dsRNA induces plant resistance against bean common mosaic virus (BCMV) F Wani, S Rashid, S Saleem, G Ali, FA Mohiddin, A Hamid Applied Biochemistry and Biotechnology 197 (5), 3431-3446 , 2025 2025 Citations: 6
A Rapid and Field-Deployable RT-LAMP Assay for Reliable Detection of Potato Virus Y (PVY) S Rashid, S Wani, N Iralu, G Ali, A Hamid Indian Journal of Microbiology, 1-9 , 2025 2025
Evaluation of Mean Performance in Agronomic and Quality Traits of Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Genotypes DSH Tinkie, U Masoodi, G Ali, N Nazir, R Rashid, G Nazir, M Rashid, ... Archives of Current Research International 25 (4), 303-313 , 2025 2025
Analysis of Genetic Variability, Correlation and Path Coefficients for Various Agronomic and Quality Traits in Bottle Gourd [Lagenaria siceraria (Molina) Standl.] Genotypes MRMM Durani Sahar Hassan Tinkie , Ummyiah H. Masoodi , Gowhar Ali , Nageena ... Journal of Experimental Agriculture International 47 (3), 264-276 , 2025 2025
Development of rapid and simple detection of bean common mosaic virus (BCMV) in mung beans (Vigna radiata) using reverse transcription-loop mediated isothermal amplification … AH Parvaiz Ullah, Shahjahan Rashid, Sumiah Wani, Nulevino Iralu, Sajad Un ... VirusDisease , 2025 2025 Citations: 1
Extraction of high-quality genomic DNA from plants using modified CTAB-based method A Javaid, S Wani, N Iralu, L Javaid, D Meghanath, G Ali, A Hamid Detection of plant viruses: Advanced techniques, 69-75 , 2025 2025 Citations: 4
Polyacrylamide Gel Electrophoresis for Visualization of Nucleic Acid A Javaid, N Iralu, S Wani, G Ali, A Hamid Detection of Plant Viruses: Advanced Techniques, 99-104 , 2025 2025 Citations: 1
Gel Electrophoresis for Visualization of Nucleic Acid A Javaid, S Wani, N Iralu, G Ali, A Hamid Detection of Plant Viruses: Advanced Techniques, 89-97 , 2025 2025
Detection of plant viruses: advanced techniques A Hamid, G Ali, A Shikari, S Saleem, SH Wani, S Wani Springer Nature , 2025 2025 Citations: 6
Correlation and path coefficient analysis of yield related traits in summer squash (Cucurbita pepo L.) M Rashid, A Amin, SB Zehra, G Ali, N Nazir, S Masood, G Nazir, ... SKUAST Journal of Research 27 (3), 445-454 , 2025 2025
MOST CITED SCHOLAR PUBLICATIONS
Gene action for grain yield and its attributes in maize (Zea mays L.) G Ali, AC Rather, A Ishfaq, SA Dar, SA Wani, MN Khan Int. J. Agric. Sci 3 (2), 278-281 , 2007 2007 Citations: 37
Studies on genetic variability and interrelationship for yield and yield component characters in chickpea (Cicer arietnum L) SA Dar, A Ishfaq, MH Khan, FA Pir, FA Gowhar, AA Manzar Trends in Biosciences 5 (2), 119-121 , 2012 2012 Citations: 33
Heterosis and combining ability for grain yield and its components in high altitude maize inbreds (Zea mays L.) G Ali, A Ishfaq, AG Rather, SA Wani, G Zaffar, MI Makhdoomi The Indian Journal of Genetics and Plant Breeding 67 (1), 81-82 , 2007 2007 Citations: 33
Combining ability for yield and maturity traits in elite inbred lines of maize ( Zea mays L.). SA Dar, GA Gowhar Ali, AG Rather, MN Khan 2007 Citations: 30
Molecular breeding for enhancing resilience against biotic and abiotic stress in major cereals. A Gazal, ZA Dar, SH Wani, AA Lone, AB Shikari, G Ali, I Abidi SABRAO Journal of Breeding & Genetics 48 (1) , 2016 2016 Citations: 24
Disease reaction studies of maize (Zea mays L.) against turcicum leaf blight involving indigenously identified cytosterile source A Ishfaq, ZA Dar, AA Lone, G Ali, A Gazal, B Hamid, FA Mohiddin African Journal of Microbiology Research 8 (27), 2592-2597 , 2014 2014 Citations: 24
Line× tester analysis in maize (Zea mays L.) for various morpho-agronomic traits under temperate conditions ZA Dar, AA Lone, NS Khuroo, G Ali, I Abidi, MA Ahangar, MA Wani, ... Int. J. Curr. Microbiol. App. Sci 6 (7), 1430-1437 , 2017 2017 Citations: 22
Prospects of clonal selection for enhancing productivity in saffron (Crocus sativus L.) G Ali, AM Iqbal, FA Nehvi, SS Samad, S Nagoo, S Naseer, NA Dar African Journal of Agricultural Research 8 (5), 460-467 , 2013 2013 Citations: 22
Genetic variability studies in cherry tomato for growth, yield, and quality traits in open field conditions K Hussain, S Lone, A Malik, KZ Masoodi, ZA Dar, N Nazir, G Ali, S Farwah International Journal of Agricultural and Applied Sciences 2 (2), 60-64 , 2021 2021 Citations: 21
Correlation studies in temperate maize (Zea mays L.) inbred lines ZA Dar, AA Lone, BA Alaie, G Ali, A Gazal, S Gulzar, N Yousuf Plant Archives 15 (2), 1191-1194 , 2015 2015 Citations: 21
Study on genetic variability, heretability and genetic advance in pea (Pisum sativum L.) SA Dar, A Ishfaq, G Ali, FA Pir, A Manzar Annals of Horticulture 6 (1), 161-163 , 2013 2013 Citations: 21
Common beans and abiotic stress challenges A Lone Current Journal of Applied Science and Technology , 2021 2021 Citations: 19
Studies on genetic variability, heritability and genetic advance in bottle gourd [Lagenaria siceraria (Molina) Standl.] genotypes M Rashid, KP Wani, K Hussain, ZA Dar, PK Singh, A Khalil, G Ali, ... Int. J. Chem. Stud 8 (3), 455-458 , 2020 2020 Citations: 19
Analysis of combining ability in maize (Zea mays L.) under temperate conditions DZ Ahmed, LA Ahmed, WS Hussain, A Bashir, A Ishfaq, A Gowhar, ... International Journal of Agriculture Sciences 9 (2), 3647-3649 , 2017 2017 Citations: 19
Genetic diversity in maize (Zea mays L.) inbred lines from Kashmir G Shazia, ZA Dar, AA Lone, MA Khan, MA Bhat, G Ali, N Yousuf, RA Lone International Journal of Pure App. Biosciences 5, 229-235 , 2017 2017 Citations: 19
Combining ability analysis for yield and yield contributing traits in Popcorn (Zea mays everta L.) under temperate conditions ZA Dar, AA Lone, BA Alie, MA Ahangar, G Ali, I Abidi, A Gazal, RA Lone J. Pharm. Phytochem 7, 361-366 , 2018 2018 Citations: 18
Distribution and molecular characterization of potato virus Y (PVY) strains infecting potato ( Solanum tuberosum ) crop in Kashmir (India) S Wani, S Saleem, SU Nabi, G Ali, BA Paddar, A Hamid VirusDisease 32 (4), 784-788 , 2021 2021 Citations: 17
Combining ability analysis for yield and its component traits in high altitude maize (Zea mays L.) inbreds G Ali, I Ahmed, SA Dar, AM Iqbal Adv. in life sci 1 (1), 66-69 , 2012 2012 Citations: 17
Genetic variability studies involving drought tolerance related traits in maize (Zea mays L.) in breds NU Islam, G Ali, ZA Dar, S Maqbool, S Baghel, A Bhat International Journal of Chemical Studies 8 (1), 414-419 , 2020 2020 Citations: 16
TILLING and Eco-TILLING–A reverse genetic approach for crop improvement A Khan, I Abidi, MA Bhat, ZA Dar, G Ali, AB Shikari, MA Khan International Journal of Current Microbiology and Applied Science 7 (06), 15-21 , 2018 2018 Citations: 16