Gretta Veronica Badillo Pazmay
@inrca.it
IRCCS INRCA - Istituto Nazionale Ricovero e Cura Anziani
RESEARCH, TEACHING, or OTHER INTERESTS
Biotechnology, Molecular Biology, Molecular Medicine, Aging
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
GianLuca Colussi, Cristiana Catena, Lorenza Driul, Francesca Pezzutto, Valentina Fagotto, Daniele Darsiè, Gretta V. Badillo-Pazmay, Giulio Romano, Paola E. Cogo, and Leonardo A. Sechi
Ovid Technologies (Wolters Kluwer Health)
Background: Preeclampsia has been associated with features of secondary hyperparathyroidism. In this study, we examine the relationships of calcium metabolism with blood pressure (BP) in preeclamptic women and in a control group of normal (NORM) pregnancies in the postpartum. Methods: Sixty-three consecutive preeclamptic women (age 35 ± 6 years) were studied 4 weeks after delivery. We collected clinical and lab information on pregnancy and neonates and measured plasma and urinary calcium and phosphate, plasma parathyroid hormone (PTH) and 25-hydroxy vitamin D [25(OH)D], and performed 24-h ambulatory BP monitoring. BP and calcium metabolism of 51 preeclamptic were compared with 17 NORM pregnant women that matched for age, race, and postpartum BMI. Results: 25(OH)D deficiency (<10 ng/ml) was found in 3% of preeclamptic women, insufficiency (10–30 ng/ml) in 67%, and NORM values (31–100 ng/ml) in the remaining 30%. Elevated plasma PTH (≥79 pg/ml) was found in 24% of preeclamptic women who had 25(OH)D plasma levels of 21.4 ± 8.3 ng/ml. In these women, PTH levels was independently associated with 24-h SBP and DBP and daytime and night-time DBP. Prevalence of nondippers and reverse dippers was elevated (75% and 33%, respectively). No associations between calcium metabolism and neonates’ characteristics of preeclamptic women were observed. Prevalence of vitamin D deficiency and insufficiency and of elevated plasma PTH levels were comparable in matched groups. Considering preeclamptic women and matched controls as a whole group, office SBP and DBP levels were associated with PTH independently of preeclampsia and other confounders. Conclusion: Features of secondary hyperparathyroidism are common in the postpartum. Preeclampsia and increased PTH levels were both independent factors associated with increased BP after delivery, and both might affect the future cardiovascular risk of these women.
Francesca D'Este, Emilia Della Pietra, Gretta Veronica Badillo Pazmay, Luigi E. Xodo, and Valentina Rapozzi
Elsevier BV
Aleksandra Wołoszyn, Claudio Pettinari, Riccardo Pettinari, Gretta Veronica Badillo Patzmay, Anna Kwiecień, Giulio Lupidi, Massimo Nabissi, Giorgio Santoni, and Piotr Smoleński
Royal Society of Chemistry (RSC)
Antiproliferative activities on human multiple myeloma cell lines of a series of novel ruthenium(ii) bpy and phen derivatives containing PTA and mPTA ligands.
Maura Montani, Gretta V. Badillo Pazmay, Albana Hysi, Giulio Lupidi, Riccardo Pettinari, Valentina Gambini, Martina Tilio, Fabio Marchetti, Claudio Pettinari, Stefano Ferraro,et al.
Elsevier BV
M. Montani, C. Marchini, G. V. B. Pazmay, C. Andreani, C. Bartolacci, A. Amici, D. Pozzi and G. Caracciolo
AIP Publishing
Intracellular delivery of reporter genes causes cells to be luminescent or fluorescent, this condition being of tremendous relevance in applied physics research. Potential applications range from the study of spatial distribution and dynamics of plasma membrane and cytosolic proteins up to the rational design of nanocarriers for gene therapy. Since efficiency of gene delivery is the main limit in most biophysical studies, versatile methods that can maximize gene expression are urgently needed. Here, we describe a robust methodology based on repeated gene delivery in mammalian cells. We find this procedure to be much more efficient than the more traditional route of gene delivery making it possible to get high-quality data without affecting cell viability. Implications for biophysical investigations are discussed.
D. Ranucci, D. Beghelli, M. Trabalza-Marinucci, R. Branciari, C. Forte, O. Olivieri, G.V. Badillo Pazmay, C. Cavallucci, and G. Acuti
Elsevier BV
Piotr Smoleński, C. Pettinari, F. Marchetti, M. F. C. Guedes da Silva, G. Lupidi, Gretta Veronica Badillo Patzmay, D. Petrelli, L. A. Vitali and A. Pombeiro
Two new silver(I) complexes of formula [Ag(mPTA)4](Tpms)4(BF4) (1) and [Ag(Tpms)(mPTA)](BF4) (2) (mPTA = N-methyl-1,3,5-triaza-7-phosphaadamantane cation, Tpms = tris(pyrazol-1-yl)methanesulfonate anion) have been synthesized and fully characterized by elemental analyses, (1)H and (31)P{(1)H} NMR, ESI-MS, and IR spectroscopic techniques. The single-crystal X-ray diffraction study of 1 discloses a noncoordinated nature of the Tpms species, existing as counterions around the highly charged metal center [Ag(mPTA)](5+), 1 being the first reported coordination compound bearing a κ(0)-Tpms. 1 features high solubility and stability in water (S25 °C ≈ 30 mg·mL(-1)). The two complexes interact with calf thymus DNA via intercalation mode, binding to the BSA with decrease of its tryptophan fluorescence with a static quenching mechanism. The two new silver complexes exhibit significant antibacterial and antifungal activities screened in vitro against the standard strains of Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans.