Marta Helena Fernandes Henriques
@ipc.pt
Instituto Politécnico Coimbra
Scopus Publications
Scopus Publications
Alexandre M.S. Jorge, Cristiana S.A. Bento, Marta H.F. Henriques, Marisa C. Gaspar, and Mara E.M. Braga
Elsevier BV
Lara Campos, Paulina Tuma, Tânia Silva, David Gomes, Carlos Dias Pereira, and Marta H. F. Henriques
MDPI AG
The food industry is looking for substitutes for sucrose in food items due to the excessive consumption of products with added sugar and the demand for healthier products. Alternative natural sweeteners can help achieve this goal. Different types of low-fat yoghurts (1% fat), with low-protein and high-protein levels (3% and 4.5–6.5% protein, respectively), were produced using alternative natural sweeteners. The low-protein yoghurts were made with stevia (0.03% w/w) or agave syrup (4.5% w/w). The high-protein yoghurts were made with stevia (0.04% w/w), xylitol (6% w/w) or honey (6% w/w). Sucrose (6% w/w) was used as a control in both trials. pH and titratable acidity, CIEL*a*b* color parameters, syneresis index, rheology and the texture profile of the low-fat yoghurts were evaluated over refrigerated storage. All products underwent sensory evaluation by an untrained panel. The high-protein yoghurts were found to be more acidic (>1% as lactic acid), had a lower syneresis index (between 2.1 and 16.2%) and a better consistency (stronger gel structure) than the low-protein yoghurts. In terms of rheological parameters, stevia-sweetened yoghurts scored higher than the other sweetened yoghurts, showing a better gel structure. The different sweeteners tested did not significantly affect the sensory properties of the yoghurts, although the high-protein yoghurts scored higher for most of the attributes evaluated. Overall, consumers preferred stevia-sweetened yoghurts to yoghurts sweetened with sucrose or agave for the low-protein yoghurts. Of the tested formulations, those containing high protein with the alternative natural sweetener xylitol received higher scores in all attributes. These results reveal the potential of the tested natural sweeteners as sucrose substitutes, while contributing to improving the nutritional value of yoghurts.
Patrícia Coimbra, Beatriz Marona, Marta H.F. Henriques, Lara Campos, David M.G.S. Gomes, Carla Vitorino, João J.S. Sousa, Mara E.M. Braga, and Marisa C. Gaspar
Elsevier BV
Alexandre M.S. Jorge, Marisa C. Gaspar, Marta H.F. Henriques, and Mara E.M. Braga
Elsevier BV
Cátia Garcia, Maria Inês Dias, Marta H. F. Henriques, Lillian Barros, and Fernando Ramos
MDPI AG
With great cultural significance, spirits and distillate beverages constitute an important niche market in Europe. The development of new food products, particularly for the functionalization of these beverages, is increasing exponentially. The present work aimed to develop a new wine spirit beverage aged with almond shells and flowers of P. tridentatum for further characterization of bioactive and phenolic compounds, coupled with a sensorial study to evaluate the acceptance of this new product by the market. Twenty-one phenolic compounds were identified, mainly isoflavonoids and O- and C-glycosylated flavonoids, especially in P. tridentatum flowers, indicating that it is a highly aromatizing agent. The developed liqueur and wine spirits (almonds and flowers) showed distinct physicochemical properties, with the last two samples showing greater appreciation and purchase intention by consumers due to their sweetness and smoothness. The most promising results were found for the carqueja flower, which should be further investigated in an industrial context to contribute to its valorization in its regions of origin, such as Beira Interior and Trás-os-Montes (Portugal).
Beatriz Oliveira, Ana Catarina Baptista, Cândida Malça, Joana Coutinho, Marta Henriques, and Carla Moura
Springer Nature Switzerland
Tânia Silva, Arona Pires, David Gomes, Jorge Viegas, Susana Pereira-Dias, Manuela E. Pintado, Marta Henriques, and Carlos Dias Pereira
MDPI AG
Small ruminant dairy products are common in some Mediterranean countries, in the Middle East and Africa, and can play a particular role in the development of rural areas. Butter has been the object of few research studies aimed at evaluating its potential as a vehicle for probiotic microorganisms. Moreover, the recovery of fermented buttermilk with functional properties can be considered an excellent opportunity to value this dairy byproduct. Therefore, the purpose of the present work was to develop different sheep butters and respective buttermilks after cream fermentation by: (1) a mesophilic aromatic starter (A); (2) a kefir culture (K); and (3) a mixture of probiotic bacteria (P). The butters and buttermilk produced with fermented cream were compared with non-fermented sweet cream (S) butter or buttermilk, respectively, regarding their physicochemical, microbiological and sensory characteristics. The adjusted production (%, w/v) obtained for butter were: S (44.48%), A (36.82%), K (41.23%) and P (43.36%). S, A and K butters had higher solids, fat and ashes contents than P butter. The probiotic butter had a total fat of ca. 75% (w/w), below the legal limits, while all others had fat levels above 81.5%. In all samples, the pH decreased and the acidity increased over 90 days of refrigerated storage. These variations were more evident in the P butter, which agrees with the highest lactic acid bacteria counts found in this sample. Differences in color between samples and due to storage time were also observed. In general, the butter samples tended to become darker and yellower after the 60th day of storage. Texture analysis showed comparable results between samples and greater hardness was observed for the P butter, most probably due to its higher relative saturated fatty acids content (66.46% compared to 62–64% in S, A and K butters). Regarding rheological properties, all butters showed pseudoplastic behavior, but butter P had the lowest consistency index (249 kPa.sn−1). The probiotic butter and the corresponding buttermilk had viable cell counts greater than 7 Log CFU/g, indicating their suitability as probiotic carriers. All products were well accepted by consumers and small, but non-significant, differences (p > 0.05) were observed in relation to the sensory parameters evaluated. In general, it can be concluded that the use of adequate starter cultures can allow the production of innovative and potentially healthier products, alongside the valorization of dairy byproducts, improving the income of small-scale producers.
Laura Ferreira, Ana Borges, David Gomes, Susana Dias, Carlos Pereira, and Marta Henriques
Wiley
Rachel Cordeiro, Marta Henriques, João C. Silva, Filipe Antunes, Nuno Alves, and Carla Moura
MDPI AG
Tissue engineering using scaffolds is a promising strategy to repair damaged articular cartilage, whose self-repair is inefficient. Cellulose properties have been recognized for their application in the biomedical field. The aim of this study was to fabricate and characterize novel scaffolds based on poly(ɛ-caprolactone) (PCL) and sustainable cellulose. Thus, the performance of corncob-derived cellulose (CC) in scaffolds as an alternative to wood cellulose (WC) was also investigated to reduce the environmental footprint. Two concentrations of CC in scaffolds were tested, 1% and 2% (w/w), and commercial WC using the same concentrations, as a control. Morphologically, all the developed scaffolds presented pore sizes of ~300 µm, 10 layers, a circular shape and well-dispersed cellulose. Thus, all of these characteristics and properties provide the manufactured scaffolds suitable for use in cartilage-replacement strategies. The use of 2% CC results in higher porosity (54.24%), which promotes cell infiltration/migration and nutrient exchange, and has similar mechanical properties to WC. As for the effects of enzymatic degradation of the scaffolds, no significant changes (p > 0.05) were observed in resistance over time. However, the obtained compressive modulus of the scaffold with 2% CC was similar to that of WC. Overall, our results suggest that the integration of 2% corncob cellulose in PCL scaffolds could be a novel way to replace wood-cellulose-containing scaffolds, highlighting its potential for cartilage-replacement strategies.
Lara Campos, Luana Seixas, Susana Dias, António M. Peres, Ana C. A. Veloso, and Marta Henriques
MDPI AG
Pomegranate by-products can be an asset to the food industry due to the richness in bioactive and antimicrobial compounds. This work studied the influence of conventional solvent and sonication-assisted extraction methods on the bioactive profile, antimicrobial properties, and phytotoxicity effect of the peels and seeds extracts from Acco, Big Full, and Wonderful pomegranate cultivars. The bioactive composition of the extracts was evaluated for the content of total phenolics, total flavonoids, and antioxidant activity (expressed as the half-maximal inhibitory concentration—IC50) by spectrophotometric methods, while the tannins were determined by titration and the anthocyanins were estimated by the pH-differential method. For the evaluation of the antimicrobial activity, the disk diffusion method of Kirby-Bauer was adapted through inhibition halos against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, and Yarrowia lipolytica. The extracts’ phytotoxicity was evaluated in vitro on garden-cress seeds. Extracts from conventional extraction were richer in total phenolics, expressed as gallic acid equivalents (0.16–0.73 mg GAE/mg extract), while those from sonication-assisted extraction had higher contents of total flavonoids, expressed as catechin equivalents (0.019–0.068 mg CATE/mg extract); anthocyanins, expressed as cyanidin-3-glucoside (0.06–0.60 µg C3G/mg, dry basis); and antioxidant activity (IC50, 0.01–0.20 mg/mL). All extracts were more effective against Gram-positive bacteria and yeasts than Gram-negative bacteria. In general, the sonication-assisted extracts led to higher inhibition halos (8.7 to 11.4 mm). All extracts presented phytotoxicity against garden-cress seeds in the tested concentrations. Only the lowest concentration (0.003 mg/mL) enabled the germination of seeds and root growth, and the sonication-assisted extracts showed the highest Munoo-Liisa vitality index (51.3%). Overall, sonication-assisted extraction obtained extracts with greater bioactive and antimicrobial potential and less phytotoxicity.
Lara Campos, Luana Seixas, Marta H. F. Henriques, António M. Peres, and Ana C. A. Veloso
Hindawi Limited
The nutraceutical properties of Punica granatum L. are not restricted to the edible portion of the fruit but also to the peels and seeds, flowers, leaves, and tree bark. The recovery and valorization of the peel and seeds (ca. 50% of the whole fruit), besides the positive environmental impact, can be viewed as a source of natural bioactive compounds. Thus, the bioactive properties of extracts of pomegranate peel and seeds from Acco and Wonderful known cultivars, as well as of the novel Big Full cultivar, were evaluated. The dried and ground pomegranate by-products were submitted to a conventional solid/liquid extraction with ethanol/water mixtures (0%, 25%, 50%, and 75% of EtOH, v/v). The obtained extracts were characterized in terms of total phenolic compounds (TPC), total flavonoids (TF), and antioxidant activity (AA), determined by the DPPH radical scavenging activity and expressed as IC50 (half maximum inhibitory concentration). With the exception of the Acco cultivar, the extraction yield (EY) was higher for peels, whose extracts showed higher TPC, TF, and IC50 (lower AA). The extracts obtained from the by-products of the Big Full cultivar had a statistically higher overall bioactive potential (TPC: 0.36 mg GAE/mg extract; TF: 0.031 mg CATE/mg extract; IC50: 0.51 mg/mL) compared to the other two studied cultivars. Furthermore, the EY was enhanced by solvents richer in ethanol (50-75%), allowing obtaining extracts richer in TPC and TF with higher AA. Finally, it was shown that EY combined with bioactive data allowed a satisfactory principal component unsupervised differentiation of the pomegranate extracts according to the type of by-product used.
Mariana Vallejo, Rachel Cordeiro, Paulo A. N. Dias, Carla Moura, Marta Henriques, Inês J. Seabra, Cândida Maria Malça, and Pedro Morouço
Springer Science and Business Media LLC
AbstractConsidering the expected increasing demand for cellulose fibers in the near future and that its major source is wood pulp, alternative sources such as vegetable wastes from agricultural activities and agro-food industries are currently being sought to prevent deforestation. In the present study, cellulose was successfully isolated from six agroindustrial residues: corncob, corn husk, grape stalk, pomegranate peel, marc of strawberry-tree fruit and fava pod. Cellulose fibers were characterized by Fourier-transform infrared spectroscopy, thermogravimetric analysis, stereomicroscopy and scanning electron microscopy (SEM). Despite the evident morphological differences among the extracted celluloses, results revealed similar compositional and thermal properties with the wood-derived commercial microcrystalline cellulose used as a control. Trace amounts of lignin or hemicellulose were detected in all cellulose samples, with the exception of corncob cellulose, that exhibited the greatest extraction yield (26%) and morphological similarities to wood-derived microcrystalline cellulose, visible through SEM. Furthermore, corncob cellulose was found to have thermal properties (TOnset of 307.17 °C, TD of 330.31 °C, and ΔH of 306.04 kJ/kg) suitable for biomedical applications.
Sylwia Chudy, Agnieszka Makowska, Mirosława Krzywdzińska‐Bartkowiak, Michał Piątek, Marta Henriques, Ana Raquel Borges, David Gomes, and Carlos Dias Pereira
Wiley
Justina Mileriene, Loreta Serniene, Marta Henriques, David Gomes, Carlos Pereira, Kristina Kondrotiene, Neringa Kasetiene, Lina Lauciene, Dalia Sekmokiene, and Mindaugas Malakauskas
American Dairy Science Association
Fresh unripened curd cheese has long been a well-known Eastern European artisanal dairy product; however, due to possible cross-contamination from manual production steps, high moisture content (50-60%), and metabolic activity of present lactic acid bacteria, the shelf life of curd cheese is short (10-20 d). Therefore, the aim of this study was to improve the shelf life of Eastern European acid-curd cheese by applying an antimicrobial protein-based (5%, wt/wt) edible coating. The bioactive edible coating was produced from liquid whey protein concentrate (a cheese production byproduct) and fortified with 0.3% (wt/wt, solution basis) Chinese cinnamon bark (Cinnamomum cassia) CO2 extract. The effect of coating on the cheese was evaluated within package-free (group 1) and additionally vacuum packaged (group 2) conditions to represent types of cheeses sold by small and big scale manufacturers. The cheese samples were examined over 31 d of storage for changes of microbiological (total bacterial count, lactic acid bacteria, yeasts and molds, coliforms, enterobacteria, Staphylococcus spp.), physicochemical (pH, lactic acid, protein, fat, moisture, color change, rheological, and sensory properties). The controlled experiment revealed that in group 1, applied coating affected appearance and color by preserving moisture and decreasing growth of yeasts and molds during prolonged package-free cheese storage. In group 2, coating did not affect moisture, color, or texture, but had a strong antimicrobial effect, decreasing the counts of yeasts and molds by 0.79 to 1.55 log cfu/g during 31 d of storage. In both groups, coating had no effect on pH, lactic acid, protein, and fat contents. Evaluated sensory properties (appearance, odor, taste, texture, and overall acceptability) of all samples were similar, indicating no effect of the coating on the flavor of curd cheese. The edible coating based on liquid whey protein concentrate with the incorporation of cinnamon extract was demonstrated to efficiently extend the shelf life of perishable fresh curd cheese, enhance its functional value, and contribute to a more sustainable production process.
Arona F Pires, Natali G Marnotes, Amal Bella, Jorge Viegas, David M Gomes, Marta H F Henriques, and Carlos J D Pereira
Wiley
BACKGROUND
In Southern European countries, whey cheeses are normally produced with ovine or caprine whey. Cow's cheese whey can also be used, but whey cheese yield is low (2-3%, w/v) which discourages its use. In the present study bovine cheese whey was concentrated by ultrafiltration for the production of four types of whey cheeses (Requeijão): conventional, without any addition (WC); with 10% (w/w) addition of cream (WCC); with cream fermented with Kefir culture (WCCK); with cream fermented with Bifidobacterium sp. culture (WCCBB12).
RESULTS
Whey cheeses with cream presented lower protein content (330-360 gkg-1 , db) and higher levels of total solids (220-250 gkg-1 ) and fat (300-330 gkg-1 , db) than WC. C16:0 and C18:1 were the most abundant fatty acids present, with 31% and 38%, respectively. The small differences found concerning instrumental determination of colour and texture, were not perceived by panelysts. However, the presence of Kefir and probiotics decreased the elastic modulus (G') of the samples, as well as their viscosity. Fermentation with Kefir presented the highest counts of LAB (7 logUFCg-1 ). However, after 14 days of refrigerated storage, the counts of yeasts and moulds reached 6 logUFCg-1 in all products, indicating the need for appropriate packaging solutions.
CONCLUSION
Ultrafiltration of bovine whey allows for the efficient production of bovine whey cheeses. The addition of cream fermented with Kefir or BB12 seems to be an efficient methodology to incorporate Kefir or probiotic bacteria in Requeijão, improving its nutritional and sensory characteristics, alongside with the potential for the extension of its shelf-life. This article is protected by copyright. All rights reserved.
Sofia Rosário Figueiredo, Ana Raquel Borges, Marta Henriques, and Ivo Rodrigues
FapUNIFESP (SciELO)
Resumo Os medronhos são frutos com um alto teor de açúcares (40%), antioxidantes e vitaminas, tais como vitamina C, β-caroteno, niacina, tocoferóis e ácidos orgânicos. No entanto, o rendimento de extração da polpa pelos métodos convencionais é baixo, correspondendo aproximadamente a 42%. Este estudo teve como objetivo avaliar o potencial uso de duas carboidrases comerciais, Viscozyme® L e Pectinex® Ultra SP-L, na extração de polpa de medronho e a sua influência nas características físico-químicas e reológicas da polpa extraída. As carboidrases foram testadas sob diferentes temperaturas (20 ºC a 25 ºC e 45 ºC), concentrações (1, 2 e 4 μL/g de fruto macerado) e tempos de reação (30 e 60 min). As mesmas condições foram aplicadas a amostras-controle sem adição de enzima. Foram determinados os rendimentos de extração (g de polpa extraída/ 100 g de fruto macerado), viscosidade aparente (a 20,0 ± 0,1 ºC), cor, acidez titulável, pH, sólidos solúveis (°Brix) e sólidos totais das polpas obtidas. A polpa com menor viscosidade aparente (0,88 ± 0,38 Pa.s) foi obtida pela aplicação da Viscozyme® L a 4 μL/g, 45 ºC, após 30 minutos de incubação. A Pectinex® Ultra SP-L foi responsável pelo rendimento de extração de polpa mais elevado (54,9 ± 1,1%) após 30 min, quando aplicada a 2 μL/g e 45 ºC. Foi também responsável pelo maior teor de sólidos solúveis (27,3 ± 0,1 °Brix), à temperatura ambiente (20 ºC a 25 ºC) após 60 minutos para a concentração 4 μL/g. Não foram encontradas diferenças significativas (p > 0,05) para as demais características físico-químicas das polpas, entre as condições testadas. A metodologia de extração de polpa assistida por carboidrases forneceu resultados promissores para a diversificação de produtos de valor acrescentado derivados do medronho, com elevado potencial de utilização na indústria alimentar (bebidas de fruta, doces e geleias, gelados, entre outros).
Łukasz Łopusiewicz, Emilia Drozłowska, Paulina Trocer, Mateusz Kostek, Mariusz Śliwiński, Marta H. F. Henriques, Artur Bartkowiak, and Peter Sobolewski
MDPI AG
Valorization of food industry waste and plant residues represents an attractive path towards obtaining biodegradable materials and achieving “zero waste” goals. Here, melanin was isolated from watermelon (Citrullus lanatus) seeds and used as a modifier for whey protein concentrate and isolate films (WPC and WPI) at two concentrations (0.1% and 0.5%). The modification with melanin enhanced the ultraviolet (UV) blocking, water vapor barrier, swelling, and mechanical properties of the WPC/WPI films, in addition to affecting the apparent color. The modified WPC/WPI films also exhibited high antioxidant activity, but no cytotoxicity. Overall, the effects were melanin concentration-dependent. Thus, melanin from watermelon seeds can be used as a functional modifier to develop bioactive biopolymer films with good potential to be exploited in food packaging and biomedical applications.
Ana Raquel Borges, Arona Figueiroa Pires, Natalí Garcia Marnotes, David Gama Gomes, Marta Fernandes Henriques, and Carlos Dias Pereira
MDPI AG
In the following study, three different dairy by-products, previously concentrated by ultrafiltration (UF), were used as ingredients in the production of reduced-fat (RF) washed curd cheeses in order to improve their characteristics. Conventional full-fat (FF) cheeses (45% fat, dry basis (db)) and RF cheeses (20–30% fat, db) were compared to RF cheeses produced with the incorporation of 5% concentrated whey (RF + CW), buttermilk (RF + CB) or sheep second cheese whey (RF + CS). Protein-to-fat ratios were lower than 1 in the FF cheeses, while RF cheeses ranged from 1.8 to 2.8. The tested by-products performed differently when added to the milk used for cheese production. The FF cheese showed a more pronounced yellow colour after 60 and 90 days of ripening, indicating that fat plays an important role regarding this parameter. As far as the texture parameters are concerned, after 60 days of ripening, RF cheeses with buttermilk presented similar results to FF cheeses for hardness (5.0–7.5 N) and chewiness (ca. 400). These were lower than the ones recorded for RF cheeses with added UF concentrated whey (RF + CW) and second cheese whey (RF + CS), which presented lower adhesiveness values. RF cheeses with 5% incorporation of buttermilk concentrated by UF presented the best results concerning both texture and sensory evaluation.
Marta Helena Fernandes HENRIQUES, David Manuel Gama Simões GOMES, Ana Raquel BORGES, and Carlos José Dias PEREIRA
FapUNIFESP (SciELO)
Katarzyna Skryplonek, Marta Henriques, David Gomes, Jorge Viegas, Catarina Fonseca, Carlos Pereira, Izabela Dmytrów, and Anna Mituniewicz-Małek
American Dairy Science Association
Frozen yogurt is a type of dairy product that is considered to be a more healthful alternative to conventional ice cream due to its lower fat content and the presence of viable lactic acid bacteria. Lactose-free products are a growing trend in the dairy industry, and lactose-free yogurts and ice creams can both be found on the market. However, lactose-free frozen yogurt has not yet reached the market. In this study, we aimed to investigate the effect of adding κ-carrageenan (0.05, 0.1, and 0.15%) and corn starch (1, 2, and 3%) on acidity, texture, viscosity, overrun, melting properties, color attributes, and sensory characteristics of lactose-free frozen yogurts. Lactose was reduced by enzymatic hydrolysis during the fermentation process. The effectiveness of the hydrolysis was measured by HPLC, and lactose was reduced to 0.05% after 80 min of incubation with the enzyme. The addition of stabilizers did not change overrun and melting properties of frozen yogurt, but it did affect pH, titratable acidity, and color parameters. The product with 0.15% κ-carrageenan had the highest hardness and stickiness values. Moreover, κ-carrageenan had a positive effect on sensory attractiveness of lactose-free frozen yogurt, and it reduced the coarse texture in comparison with the control without stabilizers. A lactose-free frozen yogurt with good quality and nutritional characteristics was produced, particularly with the use of κ-carrageenan as stabilizer.
Ana Borges, Catarina Fonseca, Filipa Carreira, Ivo Rodrigues, Marta Henriques, Ana C. A. Veloso, and António M. Peres
Springer Science and Business Media LLC
The industrial process of freezing chestnuts generates 55–60% of by-products. This study aimed to valorise these low-value by-products by producing high-value chestnut flour. Two flour production processes were evaluated, using raw (RCF) and cooked (CCF10, CCF30, CCF50) chestnut by-products during 10, 30 and 50 min. The highest production yield was obtained for RCF (43.6 ± 1.0%) followed by CCF10 (38.0 ± 1.0%). Regarding flour composition, CCF50 presented the smaller protein, fat, fibre and ash contents (P < 0.05). For colour, aw, pH and sedimentation index, no significant differences were observed namely between RCF and CCF10 (P > 0.05). The CCF10 flour also presented the smallest particle size. The highest peroxide index was found in CCF50 penalizing its acceptance by consumers due to the evidence of rancidity, emphasizing that long time-periods of cooking must be avoided. Finally, up to 129 days of storage, under no temperature or lighting controlled conditions, all chestnut flours showed to be stable overtime.
Filipa Carvalho, Ana Rodrigues, David M.G.S. Gomes, Fernanda M.L. Ferreira, Susana P. Dias, Carlos J.D. Pereira, and Marta H.F. Henriques
Elsevier