Henry Velazquez Soto

@condeinvestigacion.org

Immunology Department, Research Unit
Ophtalmology Institute Conde de Valenciana

Henry Velazquez Soto


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https://researchid.co/henryveso

RESEARCH, TEACHING, or OTHER INTERESTS

Immunology, Immunology and Allergy, Ophthalmology, General Biochemistry, Genetics and Molecular Biology
9

Scopus Publications

92

Scholar Citations

5

Scholar h-index

3

Scholar i10-index

Scopus Publications

  • Editorial: Novel and promising laboratory biomarkers for allergic disease diagnosis and prognosis: clinical applicability
    Henry Velazquez-Soto, Xin Wang, María C. Jiménez-Martínez
    Frontiers in Allergy, 2026
  • Use of atherogenic indices as predictors of cardiovascular risk in patients with irritable bowel syndrome
    Irma Y. Izaguirre-Hernández, Pablo Thomas-Dupont, Henry Velázquez-Soto, Lorenzo Islas-Vázquez, María C. Jiménez-Martínez, et al.
    Gaceta Medica De Mexico, 2024
  • Evaluation of the Abdala Vaccine: Antibody and Cellular Response to the RBD Domain of SARS-CoV-2
    Lorenzo Islas-Vazquez, Yan Carlos Alvarado-Alvarado, Marisa Cruz-Aguilar, Henry Velazquez-Soto, Eduardo Villalobos-Gonzalez, et al.
    Vaccines, 2023
    Abdala is a recently released RBD protein subunit vaccine against SARS-CoV-2. A few countries, including Mexico, have adopted Abdala as a booster dose in their COVID-19 vaccination schemes. Despite that, most of the Mexican population has received full-scheme vaccination with platforms other than Abdala; little is known regarding Abdala’s immunological features, such as its antibody production and T- and B-cell-specific response induction. This work aimed to study antibody production and the adaptive cellular response in the Mexican population that received the Abdala vaccine as a booster. We recruited 25 volunteers and evaluated their RBD-specific antibody production, T- and B-cell-activating profiles, and cytokine production. Our results showed that the Abdala vaccine increases the concentration of RBD IgG-specific antibodies. Regarding the cellular response, after challenging peripheral blood cultures with RBD, the plasmablast (CD19+CD27+CD38High) and transitional B-cell (CD19+CD21+CD38High) percentages increased significantly, while T cells showed an increased activated phenotype (CD3+CD4+CD25+CD69+ and CD3+CD4+CD25+HLA-DR+). Also, IL-2 and IFN-γ increased significantly in the supernatant of the RBD-stimulated cells. Our results suggest that Abdala vaccination, used as a booster, evokes antibody production and the activation of previously generated memory against the SARS-CoV-2 RBD domain.
  • Exogenous CFH Modulates Levels of Pro-Inflammatory Mediators to Prevent Oxidative Damage of Retinal Pigment Epithelial Cells with the At-Risk CFH Y402H Variant
    Henry Velazquez-Soto, Sergio Groman-Lupa, Marisa Cruz-Aguilar, Alberto L. Salazar, Juan C. Zenteno, et al.
    Antioxidants, 2023
    Age-related macular degeneration (AMD) is a complex, progressive degenerative retinal disease. Retinal pigment epithelial (RPE) cells play an important role in the immune defense of the eye and their dysfunction leads to the progressive irreversible degeneration of photoreceptors. Genetic factors, chronic inflammation, and oxidative stress have been implicated in AMD pathogenesis. Oxidative stress causes RPE injury, resulting in a chronic inflammatory response and cell death. The Y402H polymorphism in the complement factor H (CFH) protein is an important risk factor for AMD. However, the functional significance of CFH Y402H polymorphism remains unclear. In the present study, we investigated the role of CFH in the pro-inflammatory response using an in vitro model of oxidative stress in the RPE with the at-risk CFH Y402H variant. ARPE-19 cells with the at-risk CFH Y402H variant were highly susceptible to damage caused by oxidative stress, with increased levels of inflammatory mediators and pro-apoptotic factors that lead to cell death. Pretreatment of the ARPE-19 cell cultures with exogenous CFH prior to the induction of oxidative stress prevented damage and cell death. This protective effect may be related to the negative regulation of pro-inflammatory cytokines. CFH contributes to cell homeostasis and is required to modulate the pro-inflammatory cytokine response under oxidative stress in the ARPE-19 cells with the at-risk CFH Y402H variant.
  • Obesity Contributes to Inflammation in Patients with IBS via Complement Component 3 and C-Reactive Protein
    Pablo Thomas-Dupont, Henry Velázquez-Soto, Irma Yadira Izaguirre-Hernández, Mercedes Amieva-Balmori, Arturo Triana-Romero, et al.
    Nutrients, 2022
    Irritable Bowel Syndrome (IBS) is usually a lifelong state that disturbs the digestive system. IBS has been linked to low-grade inflammation and the release of inflammatory mediators into the bloodstream. This could be associated with the degree of obesity presented by patients with IBS. Reports imply that IBS is more frequent in obese patients than in the overall population, with a prevalence of up to 31%. Here, we evaluated the serum levels of immunological and inflammation molecules and their correlation with Body Mass Index in IBS patients and the healthy control (HC). Seventy-nine serum samples of the IBS patients and thirty-five of the HC group were analyzed to determine the levels of each molecule and compare them with their BMI. Serum levels of C3 and C4 were significantly increased in IBS patients. C3 and C4 levels were higher in IBS-M and IBS-D subtypes compared with the HC group. When patients were grouped by BMI, a positive correlation between serum C3 (r = 0.49, p < 0.0001) and CRP (r = 0.40, p < 0.001) levels was found. Our results show, for the first time, a correlation between immunological molecules and BMI in IBS patients, suggesting that the inflammatory nature of obesity could contribute to the development of the symptoms in IBS through the stimulation and release of proteins as complement components and CRP.
  • Effector-Memory B-Lymphocytes and Follicular Helper T-Lymphocytes as Central Players in the Immune Response in Vaccinated and Nonvaccinated Populations against SARS-CoV-2
    Lorenzo Islas-Vazquez, Marisa Cruz-Aguilar, Henry Velazquez-Soto, Aida Jiménez-Corona, Sonia Mayra Pérez-Tapia, et al.
    Vaccines, 2022
    Vaccines have been recognized as having a central role in controlling the COVID-19 pandemic; however, most vaccine development research is focused on IgG-induced antibodies. Here, we analyzed the generation of IgGs related to SARS-CoV-2 and the changes in B- and T-lymphocyte proportions following vaccination against COVID-19. We included samples from 69 volunteers inoculated with the Pfizer-BioNTech (BNT162b2), Astra Zeneca (AZD1222 Covishield), or Sputnik V (Gam-COVID-Vac) vaccines. IgGs related to SARS-CoV-2 increased after the first vaccine dose compared with the nonvaccinated group (Pfizer, p = 0.0001; Astra Zeneca, p < 0.0001; Sputnik V, p = 0.0089). The results of the flow cytometry analysis of B- and T-lymphocytes showed a higher proportion of effector-memory B-lymphocytes in both first and second doses when compared with the nonvaccinated subjects. FcRL4+ cells were increased in second-dose-vaccinated COVID-19(−) and recovered COVID-19(+) participants when compared with the nonvaccinated participants. COVID-19(−) participants showed a lower proportion of follicular helper T-lymphocytes (TFH) in the second dose when compared with the first-vaccine-dose and nonvaccinated subjects. In conclusion, after the first vaccine dose, immunization against SARS-CoV-2 induces IgG production, and this could be mediated by TFH and effector-memory B-lymphocytes. Our data can be used in the design of vaccine schedules to evaluate immuno-bridging from a cellular point of view.
  • Kinetic Changes in B7 Costimulatory Molecules and IRF4 Expression in Human Dendritic Cells during LPS Exposure
    Henry Velazquez-Soto, Fernanda Real-San Miguel, Sonia Mayra Pérez-Tapia, María C. Jiménez-Martínez
    Biomolecules, 2022
    A key aspect of the inflammatory phenomenon is the involvement of costimulatory molecules expressed by antigen-presenting cells (APCs) and their ability to secrete cytokines to set instructions for an adaptive immune response and to generate tolerance or inflammation. In a novel integrative approach, we aimed to evaluate the kinetic expression of the membrane and soluble B7 costimulatory molecules CD86, ICOS-L, PDL1, PDL2, the transcription factor Interferon Regulatory Factor 4 (IRF4), and the cytokines produced by monocyte-derived dendritic cells (Mo-DCs) after challenging them with different concentrations of stimulation with E. coli lipopolysaccharide (LPS) for different lengths of time. Our results showed that the stimuli concentration and time of exposure to an antigen are key factors in modulating the dynamic expression pattern of membrane and soluble B7 molecules and cytokines.
  • Activation of IL-10+ B cells: A novel immunomodulatory mechanism for therapeutic bacterial suspensions
    Alberto Salazar, Jane E Nieto, Henry Velazquez-Soto, Maria C Jiménez-Martínez
    Sage Open Medicine, 2020
    Objectives: Bacterial components are used to improve immune responses in patients with respiratory infections. Pharmacological formulations of bacterial components include a mixture of bacterial antigens, some of which are complete inactivated bacteria, that is, named bacterial suspensions; while others are fragments of bacteria, which are presented as bacterial lysates. Although bacterial lysates have been broadly used as immune-stimulators, the biological support for the therapeutic effectiveness of bacterial suspension has not yet been studied. Thus, the aim of our study was to investigate the immunological activity induced by bacterial suspension. Methods: This work was an exploratory translational study. Peripheral blood mononuclear cells were obtained from healthy donors and cultured in time–dose dependent assays with a commercial bacterial suspension. Flow cytometry was used for phenotypic analysis and for determining soluble cytokines in culture supernatants. Results: We observed that bacterial suspension activates B cells in a dose-dependent manner. Peripheral blood mononuclear cells were able to secrete IL-6 and IL-10 after 24 h of bacterial suspension stimulation. TLR2 expression was observed mainly on CD19+ CD38Lo B cells after 72 h of culture; remarkably, most of the TLR2+ CD19+ cells were also IL-10+. Conclusion: Our findings suggest that bacterial suspension induces the activation of B cell subsets as well as the secretion of IL-6 and IL-10. Expression of TLR2 on CD19+ cells could act as an activation loop of IL-10+ B regulatory cells. The clinical implications of these findings are discussed at the end of this article.
  • Low expression of il-10 in circulating bregs and inverted il-10/tnf-α ratio in tears of patients with perennial allergic conjunctivitis: A preliminary study
    Alberto Salazar, Israel Casanova-Méndez, Michele Pacheco-Quito, Henry Velázquez-Soto, Julio Ayala-Balboa, et al.
    International Journal of Molecular Sciences, 2019
    Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels.

RECENT SCHOLAR PUBLICATIONS

  • Clinical Effect of Bacterial Suspension in Patients with Moderate-Severe Allergic Conjunctivitis: A Preliminary Study
    I Casanova-Méndez, GA Quintana-Mexiac, JL Alcalá-Gallegos, ...
    Preprints , 2026
    2026
  • Soluble form of costimulatory molecules in allergic conjunctivitis
    D Fabregas, H Velazquez-Soto, PD Ramirez-Moreno, GR Vera-Duarte, ...
    Investigative Ophthalmology & Visual Science 66 (8), 1802-1802 , 2025
    2025
  • Sensitization Profiles in Allergic Conjunctivitis: A Comparative Analysis of Tear, Nasal Fluid, and Circulating Specific IgE
    EC Balcazar, PD Ramirez-Moreno, GR Vera-Duarte, H Velazquez-Soto, ...
    Investigative Ophthalmology & Visual Science 66 (8), 492-492 , 2025
    2025
  • Changes In Quality Of Life In Patients With Allergic Conjunctivitis Treated With Allergen-Specific Immunotherapy
    EVC Castro, IDC Mendez, MC Jimenez-Martinez, HV Soto, G Quintana, ...
    Investigative Ophthalmology & Visual Science 66 (8), 6120-6120 , 2025
    2025
  • Novel and promising laboratory biomarkers for allergic disease diagnosis and prognosis: clinical applicability
    H Velazquez-Soto, X Wang, MC Jiménez-Martínez
    Frontiers in Allergy 6, 1771107 , 2025
    2025
  • Use of atherogenic indices as predictors of cardiovascular risk in patients with irritable bowel syndrome
    IY Izaguirre-Hernandez, P Thomas-Dupont, H Velazquez-Soto, ...
    Gaceta médica de México 160 (6), 600-609 , 2024
    2024
  • Uso de índices aterogénicos para la predicción de riesgo cardiovascular en pacientes con síndrome de intestino irritable
    IY Izaguirre-Hernández, P Thomas-Dupont, H Velázquez-Soto, ...
    Gaceta médica de México 160 (6), 600-609 , 2024
    2024
    Citations: 2
  • Relationship of microRNAs 146a and 155 with systemic inflammatory cytokines in Age-related macular degeneration
    M Cruz-Aguilar, A Lammoglia-Kirsch, E Castillo-Balcázar, ...
    The Journal of Immunology 212 (1_Supplement), 0685_7973-0685_7973 , 2024
    2024
  • Use of atherogenic indices as predictors of cardiovascular risk in patients with irritable bowel síndrome
    IHY Irma, TD Pablo, VS Henry, IV Lorenzo, JMC María, RTM José
    Gaceta medica de Mexico 160 (6), 569-577 , 2024
    2024
    Citations: 1
  • Evaluation of the Abdala vaccine: antibody and cellular response to the RBD domain of SARS-CoV-2
    L Islas-Vazquez, YC Alvarado-Alvarado, M Cruz-Aguilar, ...
    Vaccines 11 (12), 1787 , 2023
    2023
    Citations: 3
  • Exogenous CFH modulates levels of pro-inflammatory mediators to prevent oxidative damage of retinal pigment epithelial cells with the at-risk CFH Y402H variant
    H Velazquez-Soto, S Groman-Lupa, M Cruz-Aguilar, AL Salazar, ...
    Antioxidants 12 (8), 1540 , 2023
    2023
    Citations: 9
  • Capítulo 2. Diagnóstico molecular
    M del Carmen Jiménez-Martínez, H Velázquez-Soto
    Alergia, Asma e Inmunología Pediátricas 31 (S1), 42-56 , 2023
    2023
  • Obesity contributes to inflammation in patients with IBS via complement component 3 and C-reactive protein
    P Thomas-Dupont, H Velázquez-Soto, IY Izaguirre-Hernández, ...
    Nutrients 14 (24), 5227 , 2022
    2022
    Citations: 16
  • Effector-memory B-lymphocytes and follicular helper T-lymphocytes as central players in the immune response in vaccinated and nonvaccinated populations against SARS-CoV-2
    L Islas-Vazquez, M Cruz-Aguilar, H Velazquez-Soto, A Jimenez-Corona, ...
    Vaccines 10 (10), 1761 , 2022
    2022
    Citations: 9
  • Fundamentals of Molecular Allergy: From Bench to Bedside
    H Velázquez-Soto, MCJ Martinez
    Allergic Disease-New Developments in Diagnosis and Therapy , 2022
    2022
    Citations: 2
  • Kinetic changes in B7 costimulatory molecules and IRF4 expression in human dendritic cells during LPS exposure
    H Velazquez-Soto, F Real-San Miguel, SM Pérez-Tapia, ...
    Biomolecules 12 (7), 955 , 2022
    2022
    Citations: 5
  • Historical evolution, overview, and therapeutic manipulation of co-stimulatory molecules
    H Velazquez-Soto, F Real, MC Jiménez-Martínez
    World Journal of Immunology 12 (1), 1-8 , 2022
    2022
    Citations: 5
  • Obesity Contributes to Inflammation in Patients with IBS via Complement Component 3 and C-Reactive Protein. Nutrients 2022, 14, 5227
    P Thomas-Dupont, H Velázquez-Soto, IY Izaguirre-Hernández, ...
    s Note: MDPI stays neutral with regard to jurisdictional claims in published … , 2022
    2022
  • Comparison of Inflammatory Mediators in Tears of Subjects Undergoing Refractive Surgery: Femto-LASIK Versus Small Incision Lenticule Extraction
    D Pinkus, EO Graue-Hernandez, AJ Ramírez-Miranda, H Velazquez-Soto, ...
    2020 World Cornea Congress , 2020
    2020
  • Toll like receptor 4 ligation promotes B7 family costimulatory molecules expression in a time and cytokine dependent fashion
    HV Soto, F Real, A Salazar, MC Jiménez Martínez
    The Journal of Immunology 204 (1_Supplement), 150.28-150.28 , 2020
    2020

MOST CITED SCHOLAR PUBLICATIONS

  • Allergen-based diagnostic: Novel and old methodologies with new approaches
    J Ayala-Balboa, MC Jiménez-Martínez
    Allergen, 77 , 2017
    2017
    Citations: 23
  • Obesity contributes to inflammation in patients with IBS via complement component 3 and C-reactive protein
    P Thomas-Dupont, H Velázquez-Soto, IY Izaguirre-Hernández, ...
    Nutrients 14 (24), 5227 , 2022
    2022
    Citations: 16
  • Low expression of IL-10 in circulating bregs and inverted IL-10/TNF-α ratio in tears of patients with perennial allergic conjunctivitis: a preliminary study
    A Salazar, I Casanova-Méndez, M Pacheco-Quito, H Velázquez-Soto, ...
    International journal of molecular sciences 20 (5), 1035 , 2019
    2019
    Citations: 15
  • Exogenous CFH modulates levels of pro-inflammatory mediators to prevent oxidative damage of retinal pigment epithelial cells with the at-risk CFH Y402H variant
    H Velazquez-Soto, S Groman-Lupa, M Cruz-Aguilar, AL Salazar, ...
    Antioxidants 12 (8), 1540 , 2023
    2023
    Citations: 9
  • Effector-memory B-lymphocytes and follicular helper T-lymphocytes as central players in the immune response in vaccinated and nonvaccinated populations against SARS-CoV-2
    L Islas-Vazquez, M Cruz-Aguilar, H Velazquez-Soto, A Jimenez-Corona, ...
    Vaccines 10 (10), 1761 , 2022
    2022
    Citations: 9
  • Kinetic changes in B7 costimulatory molecules and IRF4 expression in human dendritic cells during LPS exposure
    H Velazquez-Soto, F Real-San Miguel, SM Pérez-Tapia, ...
    Biomolecules 12 (7), 955 , 2022
    2022
    Citations: 5
  • Historical evolution, overview, and therapeutic manipulation of co-stimulatory molecules
    H Velazquez-Soto, F Real, MC Jiménez-Martínez
    World Journal of Immunology 12 (1), 1-8 , 2022
    2022
    Citations: 5
  • Evaluation of the Abdala vaccine: antibody and cellular response to the RBD domain of SARS-CoV-2
    L Islas-Vazquez, YC Alvarado-Alvarado, M Cruz-Aguilar, ...
    Vaccines 11 (12), 1787 , 2023
    2023
    Citations: 3
  • Uso de índices aterogénicos para la predicción de riesgo cardiovascular en pacientes con síndrome de intestino irritable
    IY Izaguirre-Hernández, P Thomas-Dupont, H Velázquez-Soto, ...
    Gaceta médica de México 160 (6), 600-609 , 2024
    2024
    Citations: 2
  • Fundamentals of Molecular Allergy: From Bench to Bedside
    H Velázquez-Soto, MCJ Martinez
    Allergic Disease-New Developments in Diagnosis and Therapy , 2022
    2022
    Citations: 2
  • Activation of IL-10+ B cells: A novel immunomodulatory mechanism for therapeutic bacterial suspensions
    A Salazar, JE Nieto, H Velazquez-Soto, MC Jiménez-Martínez
    SAGE Open Medicine 8, 2050312120901547 , 2020
    2020
    Citations: 2
  • Use of atherogenic indices as predictors of cardiovascular risk in patients with irritable bowel síndrome
    IHY Irma, TD Pablo, VS Henry, IV Lorenzo, JMC María, RTM José
    Gaceta medica de Mexico 160 (6), 569-577 , 2024
    2024
    Citations: 1
  • Clinical Effect of Bacterial Suspension in Patients with Moderate-Severe Allergic Conjunctivitis: A Preliminary Study
    I Casanova-Méndez, GA Quintana-Mexiac, JL Alcalá-Gallegos, ...
    Preprints , 2026
    2026
  • Soluble form of costimulatory molecules in allergic conjunctivitis
    D Fabregas, H Velazquez-Soto, PD Ramirez-Moreno, GR Vera-Duarte, ...
    Investigative Ophthalmology & Visual Science 66 (8), 1802-1802 , 2025
    2025
  • Sensitization Profiles in Allergic Conjunctivitis: A Comparative Analysis of Tear, Nasal Fluid, and Circulating Specific IgE
    EC Balcazar, PD Ramirez-Moreno, GR Vera-Duarte, H Velazquez-Soto, ...
    Investigative Ophthalmology & Visual Science 66 (8), 492-492 , 2025
    2025
  • Changes In Quality Of Life In Patients With Allergic Conjunctivitis Treated With Allergen-Specific Immunotherapy
    EVC Castro, IDC Mendez, MC Jimenez-Martinez, HV Soto, G Quintana, ...
    Investigative Ophthalmology & Visual Science 66 (8), 6120-6120 , 2025
    2025
  • Novel and promising laboratory biomarkers for allergic disease diagnosis and prognosis: clinical applicability
    H Velazquez-Soto, X Wang, MC Jiménez-Martínez
    Frontiers in Allergy 6, 1771107 , 2025
    2025
  • Use of atherogenic indices as predictors of cardiovascular risk in patients with irritable bowel syndrome
    IY Izaguirre-Hernandez, P Thomas-Dupont, H Velazquez-Soto, ...
    Gaceta médica de México 160 (6), 600-609 , 2024
    2024
  • Relationship of microRNAs 146a and 155 with systemic inflammatory cytokines in Age-related macular degeneration
    M Cruz-Aguilar, A Lammoglia-Kirsch, E Castillo-Balcázar, ...
    The Journal of Immunology 212 (1_Supplement), 0685_7973-0685_7973 , 2024
    2024
  • Capítulo 2. Diagnóstico molecular
    M del Carmen Jiménez-Martínez, H Velázquez-Soto
    Alergia, Asma e Inmunología Pediátricas 31 (S1), 42-56 , 2023
    2023