Preclinical screening models in anticancer drug development: strengths, limitations, and translational challenges Rishikesh Yadav, Shivam Singh, Sandeep Kumar, Arindam Kolay, Himanshu Sharma, et al. Frontiers in Pharmacology, 2026 Cancer is one of the leading causes of morbidity and mortality worldwide, accounting for a considerable global health burden and approximately 10 million deaths every year. There have been monumental breakthroughs in cancer research; but translating promising preclinical data into successful evolving drug therapies continues to have an enormous challenge. This challenge is further emphasised by the very high rate of attrition throughout clinical development, confirming the inadequacies of existing predictive screening platforms and emphasising the need for more predictive preclinical models. Therefore, this paper provides an overview of the various major types of preclinical models used to evaluate anticancer drugs, along with their respective strengths, weaknesses, and translational relevance. Classical in vitro cytotoxicity screens provide an essential element to early phase high-throughput cytotoxicity evaluations. More sophisticated 3D and 4D in vitro tumour models, patient-derived organoids, and organ-on-chip technologies allow for improved modelling of tumour structure and architecture, microenvironmental complexity, and drug penetration dynamics, improving accuracy in predicting in vivo activity of agents being screened in vitro . Further, in vivo model systems, provide essential in vivo data to dissect and understand the biology of tumours, pharmacokinetics, toxicity, and therapeutic efficacy of agents used to treat cancer. Rational drug discovery will be further enhanced through increasingly available emerging technologies such as bioinformatics, molecular docking, genomics and omics-based methods. Although traditional animal and cell line models are needed in drug discovery; the shortcomings of these systems, in their inability to reproduce the full complexity of human tumours means they account for a large proportion of translational failures. This comprehensive review shows how developing multi-faceted, human-comparable and technologically sophisticated screening platforms for test drugs will increase predictivity, speed up drug development for cancer treatment and improve clinical benefit from tested drugs.
Isolation and Characterization of Phytoconstituents from Lepidium meyenii Roots, Antioxidant Activity and Molecular Docking Analysis for Alzheimer’s Disease Himanshu Sharma, Phool Chandra Current Bioactive Compounds, 2026 Background: Alzheimer’s disease (AD) is a progressive neurodegenerative disorder characterized by memory impairment and cognitive decline. Oxidative stress and cholinergic dysfunction are key contributors to its pathogenesis. Objective: This study aimed to characterize the isolated compounds of Lepidium meyenii roots and assess its neuroprotective and anti-oxidant properties. Methods: Phytochemical screening, compound isolation, and characterization were performed on the ethyl acetate extract. Antioxidant activity was assessed using DPPH, NO, and H2O2 assays. Enzyme inhibition studies for acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were conducted in vitro. Molecular docking studies were performed to evaluate binding affinities of isolated compounds to AChE and BChE, and in vitro experiments were carried out to assess cognitive and motor function improvement. Results: The isolated compound quercetin and ergosterol exhibited significant antioxidant activity in DPPH (IC50- 35.65 ± 0.064 and 49.54 ± 0.075 μg/mL), NO (IC50- 30.48 ± 0.092 and 38.73 ± 0.030 μg/mL), and H2O2 (IC50- 24.8 ± 0.17 and 51.4 ± 0.13 μg/mL) assays. In vitro studies demonstrated potent inhibition of AChE and BChE enzymes. Molecular docking confirmed strong binding affinities of the isolated compounds with AChE and BChE. Furthermore, in vitro studies revealed that Lepidium meyenii treatment improved cognitive and motor functions progressively. Conclusion: The findings suggest that Lepidium meyenii possesses neuroprotective properties, likely mediated by its antioxidant activity and enzyme inhibition potential. These results highlight Lepidium meyenii as a promising candidate for the treatment of AD and other neurodegenerative disorders.
Applications and Scope of Microbial L-Asparaginase Rachna Shashikant Jadhav, Himanshu Sharma, Saurabh Kulshrestha Pharmaceutically Relevant Microbial Enzymes Insights into Enzymatic Diagnosis and Product Development, 2026
Mitochondria, exosome, cancer Phool Chandra, Rashmi Pathak, Himanshu Sharma, Neetu Sachan Mitochondria and Cancer from Basic Science to Clinical Applications Understanding Pathogenesis Therapy Resistance and Disease Relapse, 2026
Impact of Laser in the Manufacturing Industry Himanshu Sharma, Rashmi Pathak, Silvia Tanjum, Susmita Deb Tonni, Jaba Tahasin Samiha Treeno, et al. Laser Materials Processing and Manufacturing Techniques, 2026
Role of nanocomposite for bladder cancer management Himanshu Sharma, Abdullah Al Noman, Umme Fathima Tuz Zohora, Iftakhar Ahmad, Rashmi Pathak, et al. Bladder Cancer Breakthroughs A Global Perspective on Diagnosis Treatment and Patient Care, 2026
Enhancing Chemotherapy with Polysaccharides Himanshu Sharma, Alka Raj Pandey, Sagarika Kabra, Meghraj Vivekanand Suryawanshi, Rashmi Pathak, et al. Polysaccharides in Cancer Therapy, 2026
Materials Selection for Fused Deposition Modeling Meghraj Suryawanshi, Himanshu Sharma, Monty Datta, Abdullah Al Noman, Rashmi Pathak, et al. Advancements in Fused Deposition Modeling Fdm Technology for Pharmaceutical and Medical Applications, 2026
Overview of Dietary Polyphenols: Sources, Biochemical and Molecular Functions, and Pharmacokinetic Aspects Dietary Polyphenols for Infectious Diseases, 2025
Skin and nanotoxicity: An overview Phool Chandra, Neetu Sachan, Rashmi Pathak, Himanshu Sharma, Mohammad Akhlaquer Rahman Nanodermatology Advances in Theory and Practice, 2025
Probiotics and Their Potential Effects on Cancer Management Role of Probiotics in Cancer Management, 2025
Survivorship and Supportive Care Praveen Halagali, Vamshi Krishna Tippavajhala, Himanshu Sharma, Harpreet Singh Colorectal Cancer from Pathogenesis to Precision Medicine, 2025
Disrupted Cellular Clearance and Inflammatory Loops in Frontotemporal Dementia Praveen Halagali, Vamshi Krishna Tippavajhala, Himanshu Sharma, Adil Abalkhail, Wesam Taher Almagharbeh Neuroinflammation and Autophagy in Neurodegeneration Mechanistic Insights into Cellular Biology and Advanced Multimodal Therapeutics, 2025
Inflammatory Demyelination and Autophagic Dysfunction in Multiple Sclerosis Praveen Halagali, Salini P. Nair, S. N. Manjula, Himanshu Sharma, Vamshi Krishna Tippavajhala Neuroinflammation and Autophagy in Neurodegeneration Mechanistic Insights into Cellular Biology and Advanced Multimodal Therapeutics, 2025
Inulin as a Prebiotic and Its Effect on Gut Microbiota Amit Anand, Santhepete Nanjundiah Manjula, Neeraj Kumar Fuloria, Himanshu Sharma, Kenganora Mruthunjaya Inulin for Pharmaceutical Applications A Versatile Biopolymer, 2025
Polymeric Biomaterials for Medical Implants and Devices Himanshu Sharma, Siddhant Jai Tyagi, Prakhar Varshney, Rashmi Pathak, Sumel Ashique, et al. Polymers in Functional Foods and Drugs Chemistry and Essential Applications, 2025
Edible Biopolymers for Food Applications Meghraj Suryawanshi, Swati Kurtkoti, Taufik Mulla, Esha Shah, Himanshu Sharma, et al. Green Biopolymers for Packaging Applications, 2025
Cardiovascular Impacts of Foodborne Toxins Veeresh Sadashivanavar, Manjunath Madalageri, K. Sreedhara Ranganath Pai, Himanshu Sharma, Praveen Halagali, et al. Physiological Perspectives on Food Safety Exploring the Intersection of Health and Nutrition, 2025
Impact of Nutraceuticals on Gut Microbiota for the Management of Inflammatory Bowel Disease Inflammatory Bowel Disease and Gut Microbiota Current Strategies and Future Prospects in Nanomedicine, 2025
Role of Tumor Antigens for Cancer Vaccine Development Cancer Vaccination and Challenges Volume 1 Strategies for Therapeutic Cancer Vaccine Development, 2024
Overview of the Pharmaceutical Marketplace Pharma Marketing and Pharmacoeconomics Opportunities Challenges and the Way Forward, 2024
Introduction to Exosome and Its Role in Brain Disorders Himanshu Sharma, Hari Krishnareddy Rachamalla, Neeraj Mishra, Phool Chandra, Rashmi Pathak, et al. Exosomes Based Drug Delivery Strategies for Brain Disorders, 2024
Calcium uptake and translocation in plants Rajesh Kumar Pathak, Dev Bukhsh Singh, Himanshu Sharma, Dinesh Pandey, Seema Dwivedi Calcium Transport Elements in Plants, 2021
Swertia chirayita Vijay Singh, Vikrant Jaryan, Vikas Sharma, Himanshu Sharma, Indu Sharma, et al. Himalayan Medicinal Plants Advances in Botany Production Research, 2021
Trillium govanianum Vishal Kumar, Pradeep Singh, Pramod Kumar Singh, Mohammed Saba Rahim, Vikas Sharma, et al. Himalayan Medicinal Plants Advances in Botany Production Research, 2021
Analysis of SSR and SNP markers Ankita Mishra, Pramod Kumar Singh, Abhishek Bhandawat, Vinay Sharma, Vikas Sharma, et al. Bioinformatics Methods and Applications, 2021
Development and crosstransferability of functionally relevant microsatellite markers in Dendrocalamus latiflorus and related bamboo species Journal of Genetics, 2014
Functionally relevant novel microsatellite markers for efficient genotyping in Stevia rebaudiana Bertoni Journal of Genetics, 2014
Study of adverse effects of anti retroviral therapy in HIV naïve patients and their association with CD4 cell count Asian Journal of Pharmaceutical and Clinical Research, 2013
Separation of some co-existing industrial amino acids by normal and reversed phase thin layer chromatography on unconventional static phases Journal of the Indian Chemical Society, 2012