Synthesis of Silver and Copper oxide nanoparticles using Ficus racemosa leaf extract: characterization, anticancer potential, and dye degradation efficacy Akhilesh Kumar Maurya, Shagun Varshney, Vinod Verma, Hifzur R. Siddique, Nidhi Mishra Journal of Umm Al Qura University for Applied Sciences, 2025 Due to their economic and eco-friendly attributes, there has recently been a growing interest in phytochemicals as catalysts for synthesizing nanoparticles (NPs). The present study describes the synthesis of nanoparticles (NPs) via green chemistry by utilizing Ficus racemosa (F. racemosa) leaf extract without using any harsh chemical reagents. Silver (Ag) NPs were synthesized through conventional and microwave-irradiation routes, while the NPs of Copper oxide (CuO) were synthesized through traditional synthetic routes. The average crystallite size was around 26 and 38 nm for Ag and CuO NPs, respectively, with highly crystalline properties. The peak of around 450 nm for Ag and 551 nm for CuO, respectively, provided evidence of their formation. The NPs synthesized via the conventional route had spherical morphology with sizes between 40–80 nm and 95–250 nm for Ag and CuO NPs, respectively. With a size range of 80–300 nm, Ag NPs synthesized via microwave irradiation had triangular, polygonal, and almost spherical shapes. Ag NPs synthesized via microwave irradiation had triangular, polygonal, and nearly spherical morphology with a size range of 80–300 nm. The cytotoxicity of NPs was assessed on the human lung cancer cell line (A549). With an IC50 value of 75 µg/mL, the studies revealed that both NPs displayed comparable activities. The catalytic dye degradation activity of Ag NPs was investigated using Bromophenol blue (BB), Methylene blue (MB), and Methyl orange (MO). Therefore, using green synthesis, the current pathway facilitates the environmentally favorable synthesis of NPs for anti-cancer and dye degradation applications.
An update understanding of stemness and chemoresistance of prostate cancer Afiya Wahab, Hifzur R. Siddique Expert Review of Anticancer Therapy, 2025 INTRODUCTION: Globally, prostate cancer (CaP) is a leading cause of death and disability among men and a substantial public health burden. Despite advancements in cancer treatment, chemoresistance remains a significant issue in cancer therapy, accounting for the majority of patient relapses and poor survival. Cancer stem cells (CSCs) are considered the main cause of cancer recurrence, chemoresistance, and poor survival of patients. These CSCs acquire stemness and chemoresistance by certain mechanisms such as enhanced DNA repair processes, increased expression of drug efflux pumps, resistance to apoptosis, and altered cell cycle and tumor microenvironment (TME). AREA COVERED: We cover the latest developments in this field and give an overview of future research directions. EXPERT OPINION: CSCs show dysregulation of several signaling pathways, mostly related to conferring chemoresistance phenotype, such as high drug efflux, apoptotic resistance, quiescent cell cycle, tumor microenvironment, and DNA repair. There are several research articles published on this topic. However, still, this field warrants further investigations to identify the therapeutic molecule that can either chemosensitize CSCs or kill them effectively. This can only be possible when we know the complete mechanisms to comprehend the fundamental causes of cancer stemness and therapy resistance.
Chemosensitivity and Radiosensitivity of Triterpene Lupeol Current Status and Future Perspective Afiya Wahab, Hifzur R Siddique Lupeol Therapeutic Applications in Human Health and Disease, 2025 In cancer research, lupeol, a pentacyclic triterpene molecule present in many medicinal plants, has attracted much attention because of its strong chemotherapeutic and radiosensitizing effects. Research shows that this adaptable substance could be a viable addition to traditional cancer treatments. Lupeol's potential as a chemosensitizing and radiosensitizing agent in cancer treatment has been investigated in studies showing that it can improve the effectiveness of traditional treatments. Lupeol can target and stop the growth of several cancer cell lines while posing minimal risk to healthy cells. Lupeol's mechanisms of action in chemosensitizing and radiosensitizing include causing apoptosis, cell cycle arrest in cancer cells, suppression of angiogenesis, modulation of drug resistance, production of DNA damage, ROS and antioxidant modification, and blockage of the drug efflux pump has been thoroughly examined, demonstrating its capacity to alter important signaling pathways implicated in cell survival, proliferation, and responsiveness to cancer treatments. In order to highlight lupeol's potential as a therapeutic agent and a sensitizer for traditional cancer therapy, this chapter attempts to present an overview of the state of knowledge about its chemosensitivity and radiosensitivity in the context of cancer.
Lipopolysaccharide-induced perturbation of redox homeostasis and organ injuries: Mitigation by cholecalciferol HRS Arif Jamal Siddiqui, Homa Fatma, Mohd Jameel , Mohd Amir, Samiya Baby ... International Journal of Biological Macromolecules, 150911 , 2026 2026 Citations: 1
International guidelines on the diagnosis and treatment of NUT carcinoma Y Zhang, Q Zhang, Y Hao, J Luo, Y Piao, W Wang, Z Song, Z Li, L Brcic, ... The Innovation 7 (1) , 2026 2026 Citations: 5
The Gut Remembers:: How Food and Lifestyle Shape the Silent Rise of Colorectal Cancer SMHAHR Siddique Barak 12, 40-44 , 2026 2026
Methods to Study DNA Damage Using Drosophila melanogaster HS Chaouhan, A Sharma, HR Siddique Genotoxicity Assessment: Methods and Protocols, 449-474 , 2025 2025
Future Perspectives of Lupeol for Clinical Trials and Improvement of Activity H Fatma, M Jameel, HR Siddique Lupeol, 270-290 , 2025 2025
Lupeol: Therapeutic Applications in Human Health and Disease HR Siddique CRC Press 1, 1-308 , 2025 2025
An update understanding of stemness and chemoresistance of prostate cancer HRS Afiya Wahab Expert Review of Anticancer Therapy , 2025 2025 Citations: 4
Synthesis of Silver and Copper oxide nanoparticles using Ficus racemosa leaf extract: characterization, anticancer potential, and dye degradation efficacy AK Maurya, S Varshney, V Verma, HR Siddique, N Mishra Journal of Umm Al-Qura University for Applied Sciences 11 (3), 581-594 , 2025 2025 Citations: 12
Traditional Foods: Impact on Gut Health S Sharma, AP Singh CRC Press , 2025 2025 Citations: 2
Generative Artificial Intelligence and Large Language Models in Smart Healthcare Applications: Current Status and Future Perspectives MA Haque, HR Siddique Computational Biology and Chemistry 120, 108611 , 2025 2025 Citations: 10
Protein-Based Nanocomposites for Tissue Engineering SA Bhawani, Z Karim, M Jawaid Elsevier , 2025 2025 Citations: 2
Epigenetic Regulation of Cancer-Part D L Galluzzi, S Spada Academic Press , 2025 2025 Citations: 2
Harmful Effects of Artificial Fruits Ripening Agents on Human Health AWHR Siddique Barak 11, 24-27 , 2025 2025
Future Perspectives of Lupeol for Clinical Trials and Improvement of Activity HRS Homa Fatma, Mohd. Jameel Lupeol: Therapeutic Applications in Human health and Disease 1, 270-289 , 2025 2025
Antitumor, Anti-Angiogenesis, Anti-Invasion, and Antimetastatic Effects of Lupeol HRS Mohd. Jameel, Homa Fatma Lupeol: Therapeutic Applications in Human health and Disease 1, 161-175 , 2025 2025
Chemosensitivity and Radiosensitivity of Triterpene Lupeol: Current Status and Future Perspective AWHR Siddique Lupeol: Therapeutic Applications in Human health and Disease 1, 102-115 , 2025 2025
The Cardioprotective Effect of Pentacyclic Triterpene Lupeol Current Status and Future Prospects HRS Chohelee Choudhury, Mahuya Sengupta Lupeol: Therapeutic Applications in Human health and Disease 1, 19-32 , 2025 2025
Liver-Associated Disorders and Gut-Friendly Food H Fatma, M Jameel, HR Siddique Traditional Foods, 173-196 , 2025 2025 Citations: 1
Types, stability, biocompatibility, and toxicity of protein-based nanocomposites M Jameel, H Fatma, K Umar, HR Siddique Protein-Based Nanocomposites for Tissue Engineering, 17-36 , 2025 2025
Expert consensus and current landscape of NUT carcinoma: A comprehensive strategy from diagnosis to treatment Z Ye, X Li, F Xie, C Deng, D Yang, H Li, Y Lu, Z Mao, Q Meng, L Wang, ... Tumor Discovery 3 (4), 4904 , 2024 2024 Citations: 4
MOST CITED SCHOLAR PUBLICATIONS
Beneficial health effects of lupeol triterpene: a review of preclinical studies HR Siddique, M Saleem Life Sciences 88 (7-8), 285-293 , 2011 2011 Citations: 522
Role of BMI1, a stem cell factor, in cancer recurrence and chemoresistance: preclinical and clinical evidences HR Siddique, M Saleem Stem Cells 30 (3), 372-378 , 2012 2012 Citations: 426
S100A4 calcium-binding protein is key player in tumor progression and metastasis: preclinical and clinical evidence SK Mishra, HR Siddique, M Saleem Cancer and Metastasis Reviews 31 (1), 163-172 , 2012 2012 Citations: 218
Epigenetic Modifications of c-MYC: Role in Cancer Cell Reprogramming, Progression and Chemoresistance H Fatma, SK Maurya, HR Siddique Seminars in Cancer Biology 83, 166-176 , 2022 2022 Citations: 164
Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay HR Siddique, DK Chowdhuri, DK Saxena, A Dhawan Mutagenesis 20 (4), 285-290 , 2005 2005 Citations: 150
Lupeol, a Novel Androgen Receptor Inhibitor: Implications in Prostate Cancer Therapy HR Siddique, SK Mishra, RJ Karnes, M Saleem Clinical Cancer Research 17 (16), 5379-5391 , 2011 2011 Citations: 136
Evaluation of DNA interaction, genotoxicity and oxidative stress induced by iron oxide nanoparticles both in vitro and in vivo: attenuation by thymoquinone MO Ansari, N Parveen, MF Ahmad, AL Wani, S Afrin, Y Rahman, ... Scientific Reports 9 (1), 6912 , 2019 2019 Citations: 110
Induction of hsp70, alterations in oxidative stress markers and apoptosis against dichlorvos exposure in transgenic Drosophila melanogaster: modulation by reactive oxygen species SC Gupta, HR Siddique, N Mathur, AL Vishwakarma, RK Mishra, ... Biochimica et Biophysica Acta (BBA)-General Subjects 1770 (9), 1382-1394 , 2007 2007 Citations: 89
Hazardous effect of organophosphate compound, dichlorvos in transgenic Drosophila melanogaster (hsp70-lacZ): induction of hsp70, anti-oxidant enzymes and inhibition of … SC Gupta, HR Siddique, DK Saxena, DK Chowdhuri Biochimica et Biophysica Acta (BBA)-General Subjects 1725 (1), 81-92 , 2005 2005 Citations: 84
Differential effects of genistein on prostate cancer cells depend on mutational status of the androgen receptor AM Mahmoud, T Zhu, A Parray, HR Siddique, W Yang, M Saleem, ... PloS One 8 (10), e78479 , 2013 2013 Citations: 79
Adverse effect of organophosphate compounds, dichlorvos and chlorpyrifos in the reproductive tissues of transgenic Drosophila melanogaster: 70ákDa heat shock protein as a … SC Gupta, HR Siddique, N Mathur, RK Mishra, DK Saxena, ... Toxicology 238 (1), 1-14 , 2007 2007 Citations: 79
Apigenin in cancer prevention and therapy: A systematic review and meta-analysis of animal models D Singh, M Gupta, M Sarwat, HR Siddique Critical Reviews in Oncology/Hematology 176, 103751 , 2022 2022 Citations: 78
Mechanochemical Synthesis of Sulfur Quantum Dots for Cellular Imaging F Arshad, MP Sk, SK Maurya, HR Siddique ACS Applied Nano Materials 4 (4), 3339-3344 , 2021 2021 Citations: 78
Genotoxicity of industrial solid waste leachates in Drosophila melanogaster HR Siddique, SC Gupta, A Dhawan, RC Murthy, DK Saxena, ... Environmental and molecular mutagenesis 46 (3), 189-197 , 2005 2005 Citations: 76
NUMB phosphorylation destabilizes p53 and promotes self‐renewal of tumor‐initiating cells by a NANOG‐dependent mechanism in liver cancer HR Siddique, DE Feldman, CL Chen, V Punj, H Tokumitsu, K Machida Hepatology 62 (5), 1466-1479 , 2015 2015 Citations: 74
Superparamagnetic iron oxide nanoparticles based cancer theranostics: A double edge sword to fight against cancer MO Ansari, MF Ahmad, G Shadab, HR Siddique Journal of Drug Delivery Science and Technology 45, 177-183 , 2018 2018 Citations: 69
Revisiting Inorganic Nanoparticles as Promising Therapeutic Agents: A Paradigm Shift in Oncological Theranostics HRS Mohammad A Khan, Deepti Singh, Absar Ahmad European Journal of Pharmaceutical Sciences 164, 105892 , 2021 2021 Citations: 64
Virtual screening, ADME/T, and binding free energy analysis of anti-viral, anti-protease, and anti-infectious compounds against NSP10/NSP16 methyltransferase and main protease … SK Maurya, AK Maurya, N Mishra, HR Siddique Journal of Receptors and Signal Transduction 40 (6), 605-612 , 2020 2020 Citations: 63
Nano-enabled strategies to combat methicillin-resistant Staphylococcus aureus S Singh, A Numan, HH Somaily, B Gorain, S Ranjan, K Rilla, HR Siddique, ... Materials Science and Engineering: C 129, 112384 , 2021 2021 Citations: 54
Chemosensitization of Therapy Resistant Tumors: Targeting Multiple Cell Signaling Pathways by Lupeol, A Pentacyclic Triterpene SK Maurya, G Shadab, HR Siddique Current pharmaceutical design 26 (4), 455-465 , 2020 2020 Citations: 52
