Omar Mardenli
@alepuniv.edu.sy
Aleppo university
EDUCATION
Ph.D. reproductive biotechnology
RESEARCH INTERESTS
animal production
animal reproduction
reproductive biotechnology
animal breeding
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Hussam Aryan, Omar Mardenli, Ioan Groza, and Liviu Bogdan
National Library of Serbia
Demonstrating the role of in vitro fertilization in the equine family, the current study aimed to monitor the rates of equine in vitro-fertilized and cleaved oocytes based on microdroplet and intracytoplasmic sperm injection (ICSI) methods. The fertilization process was carried out in four main media consisting of Tyrod?s albumin lactate pyruvate and TCM-199 (1:1): F (TCM-199); assigned for the ICSI method, FI (contained 0.01 mg/ml heparin + 0.01 ng/ml hypotaurine), FII (contained 0.3 mg/ml calcium chloride + 0.1 mg/ml magnesium chloride) and FIII (contained 0.05 mg/ml hypotaurine-epinephrine). The results indicated an increase in the fertilization rate by the ICSI method (65.71%) compared to the microdroplet method (39.25%) (p 0.04). The rates of unfertilized and degenerated oocytes in the ICSI method decreased, compared to the microdroplet method, to 8.57% and 25.72%, respectively; p< 0.001. The rate of fertilized oocytes in the FI medium increased to 47.61% (p< 0.05) compared to oocytes of FII and FIII treatments (37.64% and 30.00%, respectively). On in vitro culture, the rate of cleavage increased to 74.62% in the CI medium (SOF) compared to those in the CII medium (DMEM-F12) (61.29%); p <0.001. Zygotes cultured in the CII medium achieved a higher rate of blastocyst formation (30%) compared to those in the CI medium (26.31%); p< 0.001. Applying the ICSI method and SOF culture media led to high yields of equine embryos.
Ali Sami Amin Al-Tawash, Fallah Hassan Abdel Lattif, Ali Abd Al-Jabbar Ibrahim, and Omar Mardenli
IOP Publishing
Abstract This study was conducted in the poultry field of the Department of Animal production / College of Agriculture / University of Al-Qadisiyah to know the effect of using a mixture of herbs (licorice, thyme, turmeric and red pepper) on some physiological and biochemical characteristics of quail. 120 quail were used in this study. Which were randomly distributed in four treatments (2 replicates for each treatment, 15 birds for each replicate). The mixture of herbs was added to the diet in equal proportions and with different concentrations.(T1) A mixture of medicinal herbs was used at 2% of the ration (T2) A mixture of medicinal herbs was used at 4% of the ration (T3) A mixture of medicinal herbs was used at 6% of the ration (CONTROL), The mixture of medicinal herbs was used with a percentage of 0% of the feed. Feed and water were provided freely to the birds throughout the experiment period.The effect of a mixture of herbs (licorice, thyme, turmeric and red pepper) on some physiological characteristics such as the number of red cells (RBC) and white blood cells (WBC), types of white blood cells, the packed cell volume (PCV), hemoglobin concentration (Hb) and biochemical traits such as glucose, total protein and cholesterol concentration was studied. The results indicated that there were no significant differences between the experimental treatments (medical herbal mixture) compared with the control treatment in both eosinophil and basophil white blood cells, as well as the percentage of total protein. A significant increase (p<0.05) in (red blood cell count(RBC), packed cell volume (PCV), hemoglobin percentage(Hb), white blood cell count(WBC), and white lymphocyte count, in the experimental treatments (medical herbal mixture) compared with the control treatment. There was a significant decrease in both glucose and cholesterol concentration in the experimental treatments (medical herbal mixture) compared with the control treatment. From this study we conclude that the use of the medical herbal mixture led to the improvement of some of the studied traits.
Omar Mardenli, Ali A. Ibrahim, and Ali S. A. Al- Tawash
University of Baghdad - College of Agriculture
Two experiments were conducted to study the efficiency of the ovine follicular fluid (OFF) and the age of ewe on in vitro nuclear maturation of Awassi sheep oocytes (Experiment 1) and the subsequent stages of early embryos and their quality (Experiment 2). The OFF has been added to the basic maturation medium (TCM-199) with three different levels (A: 0% (Control group), B:15%, C:25% and D:50%) while the ages of the ewes were determined into two main groups (> 2 and 1-2 year). In the first experiment, across the two age groups, matured oocytes in C solution achieved the highest (p=0.006) rates of metaphase-II (85.70 and 82.35 % respectively). In the second experiment, oocytes belonging to > 2 and 1-2-year ewe age groups (C Solution) achieved the highest (p=0.001) rates of fertilization (23.17 and 30.73% respectively). Similar trend applies to the cleavage stage, the rates reached 35.14 and 41.27% respectively (p=0.01). No significant differences were noticed at the different stages of cleavage (2-4 cell, morula, and blastocyst). The resulting embryos of B (> 2-year ewe age group) and C solution (1-2-year age group) achieved the highest (p=0.04) rates of Type1 embryos (69.56 and 76.92% respectively). A significant difference was noticed in type2 embryos (p=0.02), embryos of the first and second age groups (Solution D) achieved the highest rates (46.15 and 33.33% respectively). It can be concluded that the adding of 25% OFF to the maturation media led to significant improvement in the rates of maturation, cleavage and type1 embryos of sheep oocytes.
Ali Abd Al-Jabbar Ibrahim, Ali Sami Amin AL-Tawash, Mohammed Rasul Mahdy, Omar Mardenli, and Ghassan Al-Shammas
IOP Publishing
Abstract The main objective of this study was to find out the effect of adding Stacy’s Lamiaceae plant water extract on the traits of sperms derived from the transport channels of sheep kept cold. This study was conducted using 50 ducts of adult domestic rams slaughtered at the local Al-Shula slaughterhouse. The study was carried at the College of Agriculture, University of Al-Qadisiyah, during the period from December 2020 to March 2021. This study consists of one experiment showing the effect of adding water extract of Stachys Lamiaceae on the characteristics of chilling sperms taken from the sheep Vas Deferenses. 100 VasDeferenses were divided randomly into five groups 20 Vas deferens for each group. Spermatozoa were extracted from the Vas deferens and diluted with tris extender and egg yolk, and according to the treated groups, the control group (T1) was left untreated. The second group (T2) was added 100 international units/ml of penicillin with 100 mg/ml of streptomycin. The groups (T3;1%, T4; 2% and T5; 3%, respectively) were added to the water extract of Stachys Lamiaceae and stored in the refrigerator at 5C° for one hour across one day, two days and three days. The sperms were examined (mass activity, individual motility, progressive motility, non-progressive motility, immotile sperm, dead sperm, abnormal sperm, HOST test and water test. The results indicated a significant improvement ((P<0.05) in the parameters of sperms where sperms of T5 treatment achieved the best value compared with the control group after cold storage for one, two and three days, A significant difference was noticed (P<0.05) across the treated groups with antibiotics compared with other groups. From the current study, we conclude that the addition of the water extract of the Stachys Lamiaceae led to an improvement in the environment of sperm chilling storage, which led to an improvement in the parameters of sperm fluid.
Mahdi Saleh Mohammad Al-Kerwi, Omar Mardenli, Mohammed Rasoul Mahdi Jasim, and Moustafa Abed Al-Majeed
IOP Publishing
Abstract Air pollution in the environment in which poultry is raised is one of the most serious problems facing the poultry sector across various aspects of production. Perhaps the most dangerous gas emitted from poultry houses is ammonia. The high concentrations of this gas in the air above the permissible limits (15 ppm) will have disastrous consequences. Ammonia directly affects the health and safety of birds, as it is a cause of ammonia blindness in birds accompanied by many respiratory diseases that destroy production and increase breeding costs. In addition, high concentrations of ammonia (above 20 ppm) contribute to enhancing the infection of birds with Newcastle and the bronchitis virus. In general, the greenhouse gases emitted from poultry houses included four main gases (carbon dioxide, nitrous oxide, methane and hydrogen sulphide). Studies regarding their direct effects on the health and productivity of birds have been insufficient. In the direct form, as the concentrations of greenhouse gases rise to very high limits, they cause suffocation and death., the behaviour of the greenhouse gases in the indirect effect is reflected being a source of nutritional stress and a group of diseases and parasites which lead to a decrease in productivity levels. The intensity and concentrations of gas emissions are directly related to many factors such as geographic location, the season of the year, ventilation technologies, humidity, litter quality, nutritional status and stocking density. The advances in ventilation technologies have played a key role in expelling all harmful gases, especially those that depend on negative pressure. However, greenhouse gases remain a real threat to the poultry industry in particular and to the planet’s environment in general.
Omar Mardenli, Ali Sami Amin Al-Tawash, Ali Abd Al-Jabbar Ibrahim, Mahdi Salih Mohammad Al-Kerwi, Halil İbrahim Çetinkaya, and Ghassan Al-Shammas
IOP Publishing
Abstract “Embryo transfer” technology is one of the deterministic techniques related to the production of in vitro or in vivo produced embryos. This technique controls to a large extent the integrity of the developmental component of early embryos and their ability to develop later until birth. Pregnancy rates resulting from the transferred embryos were widely dispersed and associated with many variables and factors. The developmental stage of early embryos had a significant impact on pregnancy rates, as most studies agreed to transfer embryos in the blastocyst and morula stages due to the high rates of pregnancy (40%-100%) compared to the blastomeres. Embryo cryopreservation technology (rapid and slow) competed to a large extent with fresh embryos in pregnancy rates, even surpassing the latter in some cases, as the percentage approached 100%. The resulting pregnancy rates varied greatly (up to 95%) according to the method of transferring and hormonal induction. The results were mainly based on the size and type of the animal on the one hand and on the nature of the biological activity and the specific function of the hormones (progesterone, prostaglandin F2α, gonadotropin - releasing hormone and follicle - stimulating hormone, etc.) involved in regulating estrus in donors and recipients on the other hand. This review concluded that the embryo transfer technology has given many scenarios that cannot lead to an inevitable result in judging the efficacy of the technology of in vitro embryo production, but the results are encouraging and require further efforts.
Mahdi Saleh Mohammad, Omar Mardenli, and Ali Sami Amin AL-Tawash
IOP Publishing
Omar Mardenli, , Mahdi S. Mohammad Al-Kerwi, and Ahmad Y. Alolo
Faculty of Veterinary Medicine, University of Zagreb
In this study, two experiments were conducted to study the effect of both the follicle size and the cryoprotectants dimethyl sulfoxide (DMSO) and ethylene glycol (EG) on the main phases of nuclear maturation (Experiment I), cleavage stages and embryo quality (Experiment II) of Awassi sheep oocytes. Follicles were classified into two groups: small follicles (SF) (1-2 mm) and large follicles (LF) (> 2 mm). Oocytes were vitrified in three solutions: A (30% DMSO), B (30% EG) and C (15% DMSO and 15% EG). In Experiment I, the resulting vitrified-thawed oocytes in solution C achieved the best rates after the control group (fresh), respectively as the rates of maturation, germinal vesicle (GV), metaphase II(M-II), arrest, and lyses were 85.71% (P = 0.04), 8.33% (P = 0.02), 72.92% (P = 0.04); LF group, 15.25% (P = 0.04), and 5.08% (P = 0.04); SF group, respectively. In Experiment II, the same group of oocytes achieved the best rates after the control group, as the rates of fertilization, cleavage, 2-16 cell, Type3, blastocyst, and Type1 embryos were 63.28% (P = 0.001), 57.46% (P = 0.001), 40.38% (P = 0.04), 38.46% (P = 0.04); LF group, 30.00% (P = 0.01), and SF group 36.67% (P = 0.001), respectively, while the vitrified-thawed oocytes in A solution (SF group) reached the highest rate of Type 2 embryo quality (58.06%; P = 0.01). No significant differences were noticed in the germinal vesicle breakdown (GVBD), metaphase I (M-I) and morula stage. Vitrification of oocytes obtained from follicles with a diameter of more than 2 mm in a cocktail solution of DMSO (15%) and EG (15%) led to a significant increase in the yield and quality of the resulting sheep embryos.
Omar Mardenli, Mahdi Saleh Mohammad, and Ahmad Yaser Alolo
Indonesian Center for Animal Research and Development (ICARD)
The participatory relationship among the follicle size, follicle stimulating hormone (FSH), and cysteamine (antioxidant agent) contribute to the production of embryos characterized by abundance and good quality. The aim of this study was to evaluate the efficacy of FSH, cysteamine and follicle size on <em>in vitro</em> embryo production of Awassi sheep oocytes. Follicles sizes were determined into two groups: small follicles (1-2 mm) and large follicles (&gt; 2 mm). Oocytes were matured across two increasingly shared levels of FSH and cysteamine: A (40 ng/ml + 50 μM) and B (60 ng/ml + 100 μM). Results of the bilateral interaction showed significant differences across the follicle size (large follicles group) and the maturation treatment (B medium) in the rates of fertilization (highest value: 67.51%; p= 0.02), cleavage (highest value: 65.41%; p= 0.01), 2-16 cell stage (lowest value: 2.29%; p= 0.0001), blastocyst stage (highest value: 44.82%; p= 0.04), down to morula stage arrest (lowest value: 55.17%; p= 0.04) and Type I embryos (highest value: 52.87%; p= 0.03). Likewise, matured oocytes of small follicles group (B medium) attained the highest rate of morula stage (56.60%; p= 0.03). No significant differences were observed in Type II and Type III embryos. In order to obtain high yields of good quality embryos, it is advised to add FSH and cysteamine with levels of 60 ng/ml and 100 μM respectively to maturation medium of ovine oocytes obtained from follicles with a diameter &gt; 2 mm.
Omar R Mardenli, MS Mohammad, and HA Hassooni
Indonesian Center for Animal Research and Development (ICARD)
The use of cryoprotectants in vitrification would reduce the critical damages to the embryos, thus increase the survival rates. This research was conducted in the laboratory of reproductive biotechnology at the faculty of Agriculture of Aleppo University. The study aimed to evaluate the viability and survivability of early Syrian Awassi embryos under the influence of dimethyl sulphoxide (DMSO) and ethylene glycol (EG) following vitrification. Embryos were vitrified in three solutions of cryoprotectants (A: DMSO (3 ml), B: EG (3 ml), and C which was composed of a combination of DMSO (1.5 ml) and EG (1.5 ml)). After thawing, embryos that had been vitrified in C solution achieved the highest rates of cleavage (P&lt; 0.01) comparing with A and B solutions for 2-16 cell stage (50.00% Vs 30.77% and 36.36%), morula (9.00% Vs 44.44% and 40.00%) and blastocyst stage embryos (92.86% Vs 58.33% and 50.00%) respectively. Down to the hatching blastocyst stage, 2-16 cell stage vitrified embryos in C solution achieved an encouraging rate comparing with A and B solutions (39.20% Vs23.08% and 22.73% respectively). The rates of arrested embryos decreased significantly (P&lt; 0.05) after thawing across the three solutions especially the morula and blastocyst stage (0.00 and 3.70% respectively) (C solution). No significant differences were observed in the three types of embryos across all stages and solutions despite the large range among these rates. Given the apparent benefit of the participatory effect of cytoprotectants, it is advised to use a mixture of DMSO and EG (1:1) in vitrification of ovine embryos.