Pharmacology, Toxicology and Pharmaceutics, Pharmacy, Pharmaceutical Science, Drug Discovery
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Scopus Publications
Scopus Publications
STUDY OF THE ELEMENTAL COMPOSITION OF PARTHENOCISSUS QUINQUEFOLIA AND PARTHENOCISSUS TRICUSPIDATA RAW MATERIALS IN COMPARISON WITH THE SOIL ELEMENTAL COMPOSITION Bohdana YASHCHUK, Olena KONOVALOVA, Iryna HURTOVENKO, Mariia KALISTA, Olha SHCHERBAKOVA Phytotherapy Journal, 2025 Actuality.Considering the significance of macro-and microelements in maintaining the vital functions of a human body, it is important to determine their qualitative and quantitative composition in medicinal plant raw materials.Promising objects of study include species of the genus Parthenocissus Planch.-P.quinquefolia and P. tricuspidata, which are widespread in Ukraine and possess both decorative and pharmacological value.However, modern professional literature lacks data on the mineral element content in the raw materials of P. quinquefolia and P. tricuspidata.The raw materials of these species contain a wide range of biologically active substances, such as stilbenes, flavonoids, phenolic acids, and other substances that have antimicrobial, antioxidant, anti-inflammatory, anticancer, and senolytic activities. . 1, 2025 211 . The aim of the work -to investigate the elemental composition of P. quinquefolia and P. tricuspidata raw materials in comparison with the elemental composition of the soil.Materials and methods.Leaves and shoots of P. quinquefolia and P. tricuspidata were collected during the flowering phase (July), and fruits during full ripeness (September-October) in 2023 in Lisnyky village and Skvyra town, Kyiv Oblast (Ukraine).The study of the qualitative composition and quantitative content of mineral elements was carried out by the X-ray fluorescence method on the energy dispersive spectrometer "ElvaX-med" (Elvatech Ltd., Ukraine) in the Scientific and Technical centre "Viria Ltd." (Kyiv, Ukraine).Research results.The leaves and shoots of both species of Parthenocissus accumulated a higher total content of mineral elements compared to the fruits.The mineral content in the leaves and shoots of P. quinquefolia (2297.23 mg/100 g) was slightly higher than in P. tricuspidata (2112.52 mg/100 g) collected in Lisnyky village, and in P. tricuspidata (1650.78mg/100 g) collected in Skvyra town, significantly exceeded the content in the leaves and shoots of P. quinquefolia (524.88 mg/100 g) collected in Skvyra town.In the fruits of P. quinquefolia (1371.17mg/100 g) and P. tricuspidata (1332.20 mg/100 g) collected in Lisnyky village, the total mineral content was almost identical and somewhat lower than in the fruits of P. tricuspidata (1818.23 mg/100 g), and 2.2 times lower than in the fruits of P. quinquefolia (3097.57mg/100 g) collected in Skvyra town.The raw material of P. quinquefolia exhibited a slightly higher total content of mineral elements compared to P. tricuspidata.At the same time, P. tricuspidata was characterized by a higher sulfur content, particularly in the leaves and shoots, which may represent a species-specific feature of this raw material.Both species of Parthenocissus showed a similar elemental composition, with sulfur, potassium, and calcium prevailing regardless of the growing conditions.Neither species accumulated heavy metals (titanium, cobalt, zirconium) even in cases of significant soil concentrations, indicating the environmental safety of the raw materials.Conclusions.The results of the study of the mineral composition of P. quinquefolia and P. tricuspidata raw materials confirm the potential for further phytochemical and pharmacological studies of these raw materials to develop and implement phytomedicines with hypoglycemic, neuroprotective, anti-inflammatory, and antioxidant properties.
STUDY OF THE MINERAL ELEMENT CONTENT OF RED OAK (QUERCUS RUBRA L.) IN COMPARISON WITH SOIL Olena Konovalova, Tetiana Omelkovets, Iryna Hurtovetko, Mariia Kalısta, Olha Shcherbakova, Natalia Sydora Ankara Universitesi Eczacilik Fakultesi Dergisi, 2024 Objective: The purpose of the present work was to to determine the content of mineral elements in annual shoots with leaves and in the fruits of wild individuals of red oak (Quercus rubra L.) from two different places of growth in comparison with their content in the soil under the plants. Material and Method: The annual shoots with leaves of red oak with soil samples under plants were collected in August 2022 and fruits collected in September 2022 in Ukraine in the mixed forests near Tynne village (Rivne Oblast) and near Lisnyky village (Kyiv Oblast). The research was carried out by the X-ray fluorescence method on the “ElvaX-med” energy dispersive spectrometer. Result and Discussion: As a result of the study, both types of raw materials of Quercus rubra (annual shoots with leaves and fruits) revealed the presence of 4 macro- (S, Cl, K, Ca), 8 micro-(Mn, Fe, Cu, Zn, Br, Rb, Sr, Pb) and 4 ultramicroelements (Cr, Co, Ni, Zr). It was determined that red oak plants are concentrators of potassium and sulfur from the soil (which is indicated by the high content of these macroelements both in the soil under the plants and in all studied raw materials). A high content of calcium in the raw material of red oak from both locations was noted, and this content is apparently characteristic of the plant itself, regardless of the soil on which it grows.
STUDY ON CONTENT OF FLAVONOIDS AND ANTIOXIDANT ACTIVITY OF THE RAW MATERIALS OF PARTHENOCISSUS QUINQUEFOLIA (L.) PLANCH Olena Konovalova, Bohdana Yashchuk, Iryna Hurtovenko, Olha Shcherbakova, Mariia Kalista, Natalia Sydora Sciencerise Pharmaceutical Science, 2023 The aim of this work was to determine the component composition and quantitative content of flavonoids, including catechins, in the leaves, shoots and fruits of Virginia creeper (Parthenocissus quinquefolia (L.) Planch.) for further standardization of raw materials and establishing antioxidant activity of their water-ethanol extracts. Materials and methods. The leaves, shoots of Parthenocissus quinquefolia were collected during the phase of mass flowering in July 2020 and fruits were collected during full ripeness (September – October 2020) in Lisnyky village of Obukhiv district of Kyiv Oblast (Ukraine). Determination of the component composition and quantitative content of flavonoids, including catechins, was carried out by the method of high-performance liquid chromatography (HPLC) on an Agilent Technologies 1200 liquid chromatograph. Identification and quantitative analysis were carried out using standard solutions of flavonoids (rutin, quercetin-3-b-glycoside, naringin, neohesperidin, quercetin, naringenin, kaempferol, luteolin, apigenin) and catechins (pyrocatechin, catechin, epicatechin, epicatechin gallate and halocatechin). Antioxidant activity was determined by the spectrophotometric method at 347 nm by the ability to inhibit the autooxidation of adrenaline in vitro.Results. As a result of the study, 9 phenolic compounds were identified: rutin, quercetin, quercetin-3-b-glycoside, naringin, epicatechin, catechin, gallocatechin, epicatechin gallate. It was determined that rutin, quercetin, epicatechin, and catechin predominate in the leaves, shoots, and fruits of Virginia creeper.It was established that the extracts of leaves with shoots and fruits of Virginia creeper show a pronounced antioxidant activity due to their ability to inhibit autooxidation of adrenaline in vitro.Conclusions. The high content of rutin, quercetin, epicatechin and catechin in the leaves, shoots and fruits of Virginia creeper has scientific interest, due to discovered compounds have a valuable pharmacological effect. The obtained results can be used for the standardization of raw materials of Virginia creeper, and differences in the quantitative content of halocatechin in different types of raw materials, as well as the presence of naringin in leaves and shoots and luteolin in the fruits of Virginia creeper as diagnostic features of this species raw material.The results of the study of antioxidant activity confirm the prospects of using this raw material for the creation of new drugs with antioxidant activity
Identification of the main compounds of Agastache Foeniculum essential oil by TLC and chemotype express-determination Olena KONOVALOVA, Iryna HURTOVENKO, Tamara SHURAEVA Phytotherapy Journal, 2023 Topicality.Agastache foeniculum essential oil has bactericidal, fungicidal and anti-inflammatory effects.Depending on the dominant component of the essential oil, five Agastache chemotypes have been distinguished.So, identification of the main compounds of Agastache foeniculum essential oil in other to find markers for the chemotype determination and standardization of raw materials is advisable.The purpose of the research was to study the possibility of identifying the main terpenoids in the Agastache foeniculum raw materials by the method of "cold" thin layer chromatography (TLc) for the chemotype determination.Materials and methods.The TLc was used for investigation with ethyl acetate -toluene (1:19) as mobile phase and special conditions: a temperature of elution +15 o c and drying of chromatographic plates in a stream of cool air.Research results.Menthol, linalool and pulegone by the method of "cold" TLc, in the Agastache foeniculum raw material grown in Kyiv were identified.The predominance of pulegone allows us to attribute the raw materials to pulegone chemotype.Estragole and limonene were identified in the Agastache foeniculum raw material grown in Kherson region with a predominance of estragole that allows us to attribute this raw material to the estragole chemotype.Conclusions.The technique above of terpenoids identification ensures the specificity of the determination of the main components of Agastache foeniculum essential oil for the chemotype determination and its standardization.
INVESTIGATION OF THE POLYPHENOL COMPOSITION OF RED OAK (Quercus rubra L.) RAW MATERIALS Olena Konovalova, Tetiana Omelkovets, Iryna Hurtovenko, Natalia Sydora, Mariia Kalista, Olha Shcherbakova Sciencerise Pharmaceutical Science, 2023 The aim of the work was to determine the main groups of polyphenolic compounds in Quercus rubra L. raw materials.
 Materials and methods. The leaves and annual shoots of Northern Red Oak used for research were collected in August in Lisnyky village of Obuhiv district of Kyiv Oblast (Ukraine). Determination of the component composition and quantitative content of flavonoids (including separately catechins), hydroxycinnamic acids, and phenolic acids were carried out in the samples of air-shade-dried crushed raw material to a particle size of 3 mm by the method of high-performance liquid chromatography (HPLC). Agilent Technologies 1200 liquid chromatograph was used for liquid chromatography.Results. Using the HPLC method, 18 polyphenolic compounds were identified in leaves and annual shoots of Northern Red Oak, in particular, flavonoids: rutin, quercetin-3-β-glucoside, luteolin, neohesperidin; catechins: catechin, epicatechin, epicatechin gallate, gallocatechin; hydroxycinnamic acids: chlorogenic, caffeic, trans-ferulic, trans-cinnamic, p-coumaric, hydroxyphenylacetic, benzoic, syringic, sinapic acids; phenolic acid is gallic acid. The dominant component among flavonoids is rutin (323.43 mg/100 g) (in the composition of catechins, epicatechin gallate (25.45 mg/100 g) prevails); among hydroxycinnamic acids in Northern Red Oak raw materials, chlorogenic acid (139.62 mg/100 g) and sinapic acid (74.64 mg/100 g) prevail.Conclusions. The obtained results point to the prospects of further phytochemical and pharmacological studies of Quercus rubra raw materials, with the aim of creating new plant substances based on it with antioxidant, anti-inflammatory, and antiviral activity
STUDY OF PETIOLARY ANATOMICAL FEATURES OF COMMON OAK (QUERCUS ROBUR L.) AND RED OAK (QUERCUS RUBRA L.) AND THEIR SIGNIFICANCE FOR MICRODIAGNOSTICS OF RAW MATERIALS Olena KONOVALOVA, Olha SHCHERBAKOVA, Tetiana OMELKOVETS, Mariia KALISTA, Iryna HURTOVETKO, Natalia SYDORA, Kateryna NOVOSAD Phytotherapy Journal, 2023 Actuality.The study of petiolar anatomy of Q. rubra and Q. robur is relevant for establishing interspecies anatomical differences of leaves; its results can expand the range of diagnostic features traditionally used in pharmacognostic analysis when establishing the identity and quality of raw materials.The aim of the work was to compare the features of the petiolar anatomy of Q. rubra and Q. robur, to evaluate their diagnostic significance and the possibility of application in the pharmacognostic analysis of cut raw materials.Material and methods.The objects of the study were samples of leaves of Q. rubra and Q. robur collected in September 2023 on the territory of the M.M. Gryshko National Botanical Garden of National Academy of Sciences of Ukraine.The leaves were collected from 3-5 trees, mostly from the sunny side, at a height of 2-2.5 m.Petioles for anatomical studies were fixed and preserved in 70 % alcohol.Cross sections were examined with a SUNNY XSM-20 6500 microscope, photographed with a Sigeta MCMOS 5100 5.1 MP digital camera, and processed with ToupView v. software.3.7.Research results.The results of the study of the anatomical cross sections of the Q. rubra and Q. robur petioles in the distal, medial and proximal parts showed diagnostically significant features that can be used for species identification.In the proximal and distal parts, the general structure of Q. rubra and Q. robur the petioles appeared to be the most similar, and the species differences were the best observed in the structure of the medial part.Clear species differences in the structure of the medial part of Q. rubra and Q. robur petioles are able to be used as diagnostic features during a pharmacognostic analysis to determine the identity of the cut raw material.According to histochemical reactions, the presence of wax-like substances, lignified tissues, tannins and starch was confirmed in petioles of both species. Conclusion.It is shown that the analysis of features of the petiolar anatomy of species of the genus Quercus in pharmacognostic studies may become a convenient tool for determining the identity of species and standardization of medicinal plant raw materials, as well as being informative for the taxonomy of the genus Quercus and for elucidating the anatomical adaptations of leaves.
COMPARATIVE STUDY THE ESSENTIAL OIL COMPOSITION OF FLOWERS AND LEAVES OF CRATAEGUS MONOGYNA L. Natalia Sydora, Olena Konovalova, Svetlana Zuikina, Kateryna Semchenko, Anna Rudnyk, Iryna Hurtovenko Sciencerise Pharmaceutical Science, 2021 The aim of the work was to obtain essential oil from the leaves and flowers of the genus Hawthorn Crataegus monogyna L. and study its component composition to justify the possibility of expanding the use of this herbal drugs in medicine.
 Materials and methods. The leaves and flowers of Сrataegus monogyna L., which were used to obtain essential oil, were harvested in the western region of Ukraine in 2019. The quantitative content of essential oil in the herbal drugs was determined by hydrodistillation. By used chromato-mass spectrometry method determined the component composition of essential oil of leaves and flowers of Сrataegus monogyna L. The obtained spectra were compared with the library of mass spectra NIST05 and WILEY 2007 with a total number of spectra over 470000 in combination with programs for identification AMDIS and NIST.Results. The essential oil of leaves and flowers of Сrataegus monogyna L. was obtained and its quantitative content was determined, which was 0.15±0.02 % for flowers and 0.11±0.03 % for leaves. By used chromato-mass spectrometric method in the essential oil of flowers of Сrataegus monogyna L. revealed 37 compounds, leaves – 33. In the essential oil of flowers found monoterpenoids, the content of which was 0.44 % of the total, triterpenoids 7.17 %, aromatic compounds 12.61 %. In the essential oil of leaves were found monoterpenoids 37.8 %, aromatic compounds 10.25 %, norterpenoids 3.62 %, sesquiterpenoids 4.02 %, diterpenoids 2.17 %, triterpenoids 5.08 %.Conclusions. Due to the defined component composition of the essential oil of flowers and leaves of Сrataegus monogyna L., we consider its use to create drugs of external action for the treatment of skin diseases of various etiologies caused by staphylococcal and fungal microflora.
