Indumathi Sathisaran

@nitc.ac.in

Assistant Professor, School of Biotechnology
National Institute of Technology Calicut



                       

https://researchid.co/indumathi.sathisaran

EDUCATION

B.Tech, M.Tech, PhD

RESEARCH, TEACHING, or OTHER INTERESTS

Multidisciplinary, Biotechnology, Cancer Research, Biomaterials

13

Scopus Publications

532

Scholar Citations

10

Scholar h-index

10

Scholar i10-index

Scopus Publications

  • A review of precision medicine in developing pharmaceutical products: Perspectives and opportunities
    Jorge E. Martínez-Jiménez, Indumathi Sathisaran, Francheska Reyes Figueroa, Stephanie Reyes, Marisol López-Nieves, Cornelis P. Vlaar, Jean-Christophe M. Monbaliu, Rodolfo Romañach, Gualberto Ruaño, Torsten Stelzer,et al.
    Elsevier BV

  • 3D printing and bioprinting in the battle against diabetes and its chronic complications
    Indumathi Sathisaran
    Frontiers Media SA
    Diabetes is a metabolic disorder characterized by high blood sugar. Uncontrolled blood glucose affects the circulatory system in an organism by intervening blood circulation. The high blood glucose can lead to macrovascular (large blood vessels) and microvascular (small blood vessels) complications. Due to this, the vital organs (notably brain, eyes, feet, heart, kidneys, lungs and nerves) get worsen in diabetic patients if not treated at the earliest. Therefore, acquiring treatment at an appropriate time is very important for managing diabetes and other complications that are caused due to diabetes. The root cause for the occurrence of various health complications in diabetic patients is the uncontrolled blood glucose levels. This review presents a consolidated account of the applications of various types of three-dimensional (3D) printing and bioprinting technologies in treating diabetes as well as the complications caused due to impaired blood glucose levels. Herein, the development of biosensors (for the diagnosis), oral drug formulations, transdermal drug carriers, orthotic insoles and scaffolds (for the treatment) are discussed. Next to this, the fabrication of 3D bioprinted organs and cell-seeded hydrogels (pancreas engineering for producing insulin and bone engineering for managing bone defects) are explained. As the final application, 3D bioprinting of diabetic disease models for high-throughput screening of ant-diabetic drugs are discussed. Lastly, the challenges and future perspective associated with the use of 3D printing and bioprinting technologies against diabetes and its related chronic complications have been put forward.

  • Cocrystallization of an Antiretroviral Drug Nevirapine: An Eutectic, a Cocrystal Solvate, and a Cocrystal Hydrate
    Indumathi Sathisaran and Sameer Vishvanath Dalvi
    American Chemical Society (ACS)
    Nevirapine (NEV) is an antiretroviral drug which falls under the Biopharmaceutics Classification System (BCS) class II category. In this work, we report the synthesis of new eutectic and cocrystal ...


  • Investigating Cocrystallization of Carbamazepine with Structurally Compatible Coformers: New Cocrystal and Eutectic Phases with Enhanced Dissolution
    Indumathi Sathisaran and Sameer Vishvanath Dalvi
    Springer Science and Business Media LLC
    In this work, carbamazepine (CBZ), an anticonvulsant drug was cocrystallized with several structurally complement coformers (coformers with amide, acid and hydrazide functional groups) to enhance its dissolution. CBZ formed a cocrystal phase with acetamide (ACE) when mixtures of CBZ and ACE (containing CBZ mole fractions, X CBZ of 0.25, 0.33, 0.5, and 0.67) were subjected to solid-state grinding (SSG), evaporative crystallization (EC), slurry conversion (SC), and slow cooling crystallization (SLC). Upon heating, the CBZ-ACE cocrystal phase formed from CBZ-ACE mixtures containing X CBZ of 0.25, 0.33 and 0.67 underwent solid-state phase transition to CBZ form I and CBZ cocrytsal phase obtained from the CBZ-ACE mixture containing X CBZ of 0.5 converted to CBZ form III. Interestingly, slow cooling cocrystallization experiments resulted in crystallization of a cocrystal as well as the CBZ dihydrate forms. The powder dissolution studies demonstrated that among the different CBZ-ACE-SSG cocrystal phases, CBZ-ACE-SSG-X CBZ -0.33 cocrystal exhibited 7.47 times improved dissolution whereas the CBZ eutectic phase with nicotinic acid hydrazide (NAH) exhibited 4.93 times increased dissolution when compared to raw CBZ.

  • Physical characterization of chitosan/gelatin-alginate composite beads for controlled release of urea
    Indumathi Sathisaran and Murugesan Balasubramanian
    Elsevier BV
    Polymer-based controlled-release formulations are gaining significant advantage over chemical fertilizers in recent years as they contribute to the preservation of soil fertility by reducing soil pollution in farm lands. In this work, urea (a nitrogen source fertilizer) has been entrapped within chitosan-alginate and gelatin-alginate composite beads at three different concentrations. The physical properties of the polymer composite beads namely the diameter, porosity, yield percentage, Carr's index and Hausner's ratio were determined. These fertilizer-loaded beads were also characterized by Scanning Electron Microscopy (SEM) and Fourier Transform-Infra Red (FT-IR) spectroscopy. Urea enhanced swelling of chitosan-alginate beads through the creation of pores whereas in the case of gelatin-alginate formulations, urea decreased the swelling. The swelling of the polymer composite beads was found to be maximum at pH of 5.6 when compared to that of pH conditions, 7 and 8.5. The chitosan-alginate composite beads were found to possess better fertilizer entrapping efficiency than the gelatin-alginate composite beads. The in vitro urea release studies demonstrated that the urea-entrapped gelatin-alginate beads exhibited slower urea release than that of the chitosan-alginate beads. These controlled release urea formulations were found to follow quasi-fickian diffusion mechanism.

  • Effectiveness of oil-layered albumin microbubbles produced using microfluidic t-junctions in series for in vitro inhibition of tumor cells
    Aaqib H. Khan, Xinyue Jiang, Swarupkumar Surwase, Merve Gultekinoglu, Cem Bayram, Indumathi Sathisaran, Dhiraj Bhatia, Jubair Ahmed, Bingjie Wu, Kezban Ulubayram,et al.
    American Chemical Society (ACS)
    This work focuses on evaluation of the effectiveness of double layered microbubbles loaded with doxorubicin and curcumin for cell invasion arrest from 3D spheroid models of triple negative breast cancer (TNBC), MDA-MB-231 cell line. Albumin microbubbles coated in drug-laden oil layer were synthesized using a new method of connecting two microfluidic T-mixers in series. Double-layered microbubbles thus produced consist of an innermost core of nitrogen gas encapsulated in an aqueous layer of bovine serum albumin (BSA) which in turn, is coated with an outer layer of silicone oil. In order to identify the process conditions leading to the formation of double-layered microbubbles, a regime map was constructed based on Capillary numbers for aqueous and oil phases. The microbubble formation regime transitions from double-layered to single layer microbubbles and then to formation of single oil droplets upon gradual change in flow rates of aqueous and oil phases. In-vitro dissolution studies of double-layered microbubbles in an air-saturated environment indicated that a complete dissolution of such bubbles produces an oil droplet devoid of gas bubble. Incorporation of doxorubicin and curcumin was found to produce a synergistic effect, which resulted in higher cell deaths in 2D monolayers of TNBC cells and inhibition of cell proliferation from 3D spheroid models of TNBC cells compared to control.

  • New curcumin-trimesic acid cocrystal and anti-invasion activity of curcumin multicomponent solids against 3D tumor models
    Indumathi Sathisaran, Dhiraj Devidas Bhatia, and Sameer Vishvanath Dalvi
    Elsevier BV
    Curcumin (CUR) is a Biopharmaceutics Classification System (BCS) class IV drug with poor aqueous solubility and low permeability. The dissolution of CUR can be enhanced through the cocrystallization approach. In this work, we report a new cocrystal phase of CUR with trimesic acid (TMA) with the enhanced dissolution of CUR. Cytotoxicity and cell invasion assays were conducted on (2D) monolayers and three-dimensional (3D) tumor models of triple-negative breast cancer (TNBC) cells, MDA-MB-231 using the new CUR-TMA cocrystal phase along with different CUR solid forms prepared in our previous works. The cytotoxicity and internalization assays conducted on 2D monolayers indicated that all CUR multicomponent solid forms except Curcumin-Folic Acid Dihydrate (CUR-FAD) (1:1) coamorphous solid exhibited enhanced bioavailability than unprocessed CUR. Cell invasion assay conducted on 3D tumor spheroid models showed that Curcumin-Hydroxyquinol (CUR-HXQ) cocrystal completely inhibited cell invasion whereas CUR-FAD (1:1) coamorphous solid induced enhanced invasion of cells from spheroid models.

  • Cocrystallization of carbamazepine with amides: Cocrystal and eutectic phases with improved dissolution
    Indumathi Sathisaran and Sameer Vishvanath Dalvi
    Elsevier BV
    Abstract Carbamazepine is an anticonvulsant drug which belongs to Biopharmaceutics Classification System (BCS) Class II drugs. In this work, attempts have been made to enhance the dissolution of carbamazepine (CBZ) in aqueous medium by attempting cocrystallization with the coformers such as para-hydroxybenzamide (PHBAD), salicylamide (SAL) and pyrazinamide (PRZ). Binary phase diagrams were constructed by conducting Differential Scanning Calorimetry (DSC) analysis for these CBZ-coformer pairs in order to determine the nature of the solid phase and cocrystal forming zone. CBZ formed a 1:1 cocrystal phase with PHBAD from the eutectic melts at a stoichiometric ratio of 1:1 and 1:2 whereas it formed eutectics with SAL as well as PRZ. Single crystals of CBZ-PHBAD (1:1) cocrystal phase were obtained by solvent evaporation of CBZ-PHBAD eutectic melts in Acetone-Toluene (1:1 vol ratio). Interestingly, recrystallization of eutectic melt of CBZ-PHBAD (1:1) in 1,4-Dioxane produced crystals of para-hydroxybenzamide monohydrate. Crystal structure analysis showed that CBZ-PHBAD (1:1) cocrystal and para-hydroxybenzamide monohydrate crystallized in monoclinic space groups namely P21/c and P21/n respectively. CBZ formed intermolecular interaction with two molecules of PHBAD via O-H…O hydrogen bonding and N-H...O intermolecular interaction which results into a cocrystal. Powder Dissolution (PD) studies were conducted for the CBZ-PHBAD (1:1) cocrystal, CBZ-SAL and CBZ-PRZ eutectic mixtures in Phosphate Buffer Saline (PBS) at 37 °C. Among the three carbamazepine solid forms, CBZ-PHBAD cocrystal exhibited 6.5 times higher dissolution than raw carbamazepine while the CBZ-SAL-SSG-XCBZ-0.5 eutectic and CBZ-PRZ-SSG-XCBZ-0.5 eutectic phases exhibited 2.62 and 3.05 times enhanced dissolution than raw carbamazepine.

  • Curcumin Eutectics with Enhanced Dissolution Rates: Binary Phase Diagrams, Characterization, and Dissolution Studies
    Indumathi Sathisaran, Jenna Marie Skieneh, Sohrab Rohani, and Sameer Vishvanath Dalvi
    American Chemical Society (ACS)
    Curcumin is a potentially viable pharmaceutical ingredient obtained from the rhizome of a turmeric plant, Curcuma longa. It is a polyphenolic compound which is known to possess antibacterial, anti-...

  • Engineering cocrystals of poorlywater-soluble drugs to enhance dissolution in aqueous medium
    Indumathi Sathisaran and Sameer Dalvi
    MDPI AG
    Biopharmaceutics Classification System (BCS) Class II and IV drugs suffer from poor aqueous solubility and hence low bioavailability. Most of these drugs are hydrophobic and cannot be developed into a pharmaceutical formulation due to their poor aqueous solubility. One of the ways to enhance the aqueous solubility of poorlywater-soluble drugs is to use the principles of crystal engineering to formulate cocrystals of these molecules with water-soluble molecules (which are generally called coformers). Many researchers have shown that the cocrystals significantly enhance the aqueous solubility of poorly water-soluble drugs. In this review, we present a consolidated account of reports available in the literature related to the cocrystallization of poorly water-soluble drugs. The current practice to formulate new drug cocrystals with enhanced solubility involves a lot of empiricism. Therefore, in this work, attempts have been made to understand a general framework involved in successful (and unsuccessful) cocrystallization events which can yield different solid forms such as cocrystals, cocrystal polymorphs, cocrystal hydrates/solvates, salts, coamorphous solids, eutectics and solid solutions. The rationale behind screening suitable coformers for cocrystallization has been explained based on the rules of five i.e., hydrogen bonding, halogen bonding (and in general non-covalent bonding), length of carbon chain, molecular recognition points and coformer aqueous solubility. Different techniques to screen coformers for effective cocrystallization and methods to synthesize cocrystals have been discussed. Recent advances in technologies for continuous and solvent-free production of cocrystals have also been discussed. Furthermore, mechanisms involved in solubilization of these solid forms and the parameters influencing dissolution and stability of specific solid forms have been discussed. Overall, this review provides a consolidated account of the rationale for design of cocrystals, past efforts, recent developments and future perspectives for cocrystallization research which will be extremely useful for researchers working in pharmaceutical formulation development.

  • Co-amorphous form of curcumin-folic acid dihydrate with increased dissolution rate
    Jenna Marie Skieneh, Indumathi Sathisaran, Sameer Vishvanath Dalvi, and Sohrab Rohani
    American Chemical Society (ACS)
    Curcumin is a naturally occurring compound derived from turmeric. Despite its many medicinal properties, such as being an antioxidant, anti-inflammatory, tumor reducer, etc., applications of curcumin are restricted due to its low aqueous solubility and consequently its poor bioavailability. By converting the solid state of poorly water-soluble active pharmaceutical ingredients to coamorphous mixtures, solvates, cocrystals, and eutectics, the solubility can be significantly improved. In this study, U. S. Food and Drug Administration approved excipients were screened for their ability to form novel solid states with curcumin to increase its aqueous solubility. Excipients were screened based on their molecular complementarity with curcumin, using Mercury software. Folic acid dihydrate (FAD), suberic acid, and dextrose are the three coformers that are investigated in this study. It was found that a coamorphous mixture can be formed between curcumin and FAD. FAD has potential as a prenatal or a women’s health ...

  • Crystal Engineering of Curcumin with Salicylic Acid and Hydroxyquinol as Coformers
    Indumathi Sathisaran and Sameer Vishvanath Dalvi
    American Chemical Society (ACS)
    Curcumin is a pharmaceutically viable ingredient derived from the rhizome of the Indian spice turmeric (Curcuma longa). However, curcumin suffers from poor water solubility, which limits its bioavailability. In this work, we report studies carried out to investigate cocrystallization of curcumin to improve its aqueous solubility. Salicylic acid and hydroxyquinol were used as coformers. Binary phase diagrams were constructed for curcumin–salicylic acid and curcumin–hydroxyquinol systems using differential scanning calorimetric (DSC) thermograms obtained for mixtures prepared by solid-state grinding. The curcumin–salicylic acid system was found to form an eutectic at a curcumin mole fraction of 0.33, whereas the curcumin–hydroxyquinol system clearly exhibited a cocrystal forming region. Out of the several curcumin to hydroxyquinol ratios studied, cocrystal formation was observed for mixtures containing curcumin mole fractions of 0.33 and 0.5. These curcumin–hydroxyquinol cocrystals were further characterize...

RECENT SCHOLAR PUBLICATIONS

  • A review of precision medicine in developing pharmaceutical products: Perspectives and opportunities
    JE Martinez-Jimenez, I Sathisaran, F Reyes-Figueroa, S Reyes, ...
    International Journal of Pharmaceutics 670, 125070 2025

  • 3D Printing and Bioprinting in the Battle Against Diabetes and its Chronic Complications
    I Sathisaran
    Frontiers in Bioengineering and Biotechnology 12, 1363483 2024

  • Acknowledgment to the Reviewers of Agriculture in 2022
    Agriculture Editorial Office
    Agriculture 13 (2), 250 2023

  • Acknowledgment to the Reviewers of Crystals in 2022
    Crystals Editorial Office
    Crystals 13 (2), 179 2023

  • Acknowledgment to the Reviewers of Pharmaceuticals in 2022
    PE Office
    Pharmaceuticals 16 (2), 137 2023

  • Preparation and Characterization of Modafinil Nanofibers by Electrospinning
    I Sathisaran, JC Monbaliu, C Vlaar, J Duconge, V Lopez-Mejias, T Stelzer
    2022 AIChE Annual Meeting 2022

  • Crystal engineering of nevirapine for improved dissolution characteristics: an interesting behavior of a coformer during evaporative cocrystallization
    I Sathisaran, SV Dalvi
    Crystal Engineering Gordon Research Conference 2022

  • Acknowledgment to Reviewers of Pharmaceuticals in 2021
    Pharmaceuticals Editorial Office
    Pharmaceuticals 15 (2), 184 2022

  • Cocrystallization of an antiretroviral drug nevirapine: an eutectic, a cocrystal solvate, and a cocrystal hydrate
    I Sathisaran, SV Dalvi
    Crystal Growth & Design 21 (4), 2076-2092 2021

  • Correction to: Investigating cocrystallization of carbamazepine with structurally compatible coformers: new cocrystal and eutectic phases with enhanced dissolution
    I Sathisaran, SV Dalvi
    AAPS PharmSciTech 22, 1-1 2021

  • Investigating cocrystallization of carbamazepine with structurally compatible coformers: new cocrystal and eutectic phases with enhanced dissolution
    I Sathisaran, SV Dalvi
    AAPS PharmSciTech 22 (1), 1-14 2021

  • Physical characterization of chitosan/gelatin-alginate composite beads for controlled release of urea
    I Sathisaran, M Balasubramanian
    Heliyon 6 (11), e05495 2020

  • Crystal engineering of pooly water-soluble drugs
    I Sathisaran
    Indian Institute of Technology Gandhinagar 2020

  • New curcumin-trimesic acid cocrystal and anti-invasion activity of curcumin multicomponent solids against 3D tumor models
    I Sathisaran, DD Bhatia, SV Dalvi
    International Journal of Pharmaceutics 587, 119667 2020

  • Effectiveness of oil-layered albumin microbubbles produced using microfluidic T-junctions in series for in vitro inhibition of tumor cells
    AH Khan, X Jiang, S Surwase, M Gultekinoglu, C Bayram, I Sathisaran, ...
    Langmuir 36 (39), 11429-11441 2020

  • Evaluation of anti-cancer and anti-invasion activity of curcumin multicomponent solids against 2D monolayer culture and 3D tumor model of a Triple Negative Breast Cancer (TNBC
    I Sathisaran, D Bhatia, SV Dalvi
    Indian Society for Chemists and Biologists Conference - NIPiCON 2020

  • Cocrystallization of carbamazepine with amides: Cocrystal and eutectic phases with improved dissolution
    I Sathisaran, SV Dalvi
    Journal of Molecular Structure 1193, 398-415 2019

  • Curcumin eutectics with enhanced dissolution rates: binary phase diagrams, characterization, and dissolution studies
    I Sathisaran, JM Skieneh, S Rohani, SV Dalvi
    Journal of Chemical & Engineering Data 63 (10), 3652-3671 2018

  • Engineering cocrystals of poorly water-soluble drugs to enhance dissolution in aqueous medium
    I Sathisaran, SV Dalvi
    Pharmaceutics 10 (3), 108 2018

  • Enhancing aqueous solubility of carbamazepine by crystal engineering approach
    I Sathisaran, SV Dalvi
    Asian Crystallization Technology Symposium 2018

MOST CITED SCHOLAR PUBLICATIONS

  • Engineering cocrystals of poorly water-soluble drugs to enhance dissolution in aqueous medium
    I Sathisaran, SV Dalvi
    Pharmaceutics 10 (3), 108 2018
    Citations: 202

  • Crystal engineering of curcumin with salicylic acid and hydroxyquinol as coformers
    I Sathisaran, SV Dalvi
    Crystal Growth & Design 17 (7), 3974-3988 2017
    Citations: 88

  • Physical characterization of chitosan/gelatin-alginate composite beads for controlled release of urea
    I Sathisaran, M Balasubramanian
    Heliyon 6 (11), e05495 2020
    Citations: 58

  • Co-amorphous form of curcumin–folic acid dihydrate with increased dissolution rate
    JM Skieneh, I Sathisaran, SV Dalvi, S Rohani
    Crystal Growth & Design 17 (12), 6273-6280 2017
    Citations: 56

  • Cocrystallization of carbamazepine with amides: Cocrystal and eutectic phases with improved dissolution
    I Sathisaran, SV Dalvi
    Journal of Molecular Structure 1193, 398-415 2019
    Citations: 27

  • Effectiveness of oil-layered albumin microbubbles produced using microfluidic T-junctions in series for in vitro inhibition of tumor cells
    AH Khan, X Jiang, S Surwase, M Gultekinoglu, C Bayram, I Sathisaran, ...
    Langmuir 36 (39), 11429-11441 2020
    Citations: 22

  • New curcumin-trimesic acid cocrystal and anti-invasion activity of curcumin multicomponent solids against 3D tumor models
    I Sathisaran, DD Bhatia, SV Dalvi
    International Journal of Pharmaceutics 587, 119667 2020
    Citations: 21

  • Curcumin eutectics with enhanced dissolution rates: binary phase diagrams, characterization, and dissolution studies
    I Sathisaran, JM Skieneh, S Rohani, SV Dalvi
    Journal of Chemical & Engineering Data 63 (10), 3652-3671 2018
    Citations: 21

  • Investigating cocrystallization of carbamazepine with structurally compatible coformers: new cocrystal and eutectic phases with enhanced dissolution
    I Sathisaran, SV Dalvi
    AAPS PharmSciTech 22 (1), 1-14 2021
    Citations: 17

  • Cocrystallization of an antiretroviral drug nevirapine: an eutectic, a cocrystal solvate, and a cocrystal hydrate
    I Sathisaran, SV Dalvi
    Crystal Growth & Design 21 (4), 2076-2092 2021
    Citations: 14

  • National Institute of Technology Calicut
    I Sathisaran
    India
    Citations: 2

  • A review of precision medicine in developing pharmaceutical products: Perspectives and opportunities
    JE Martinez-Jimenez, I Sathisaran, F Reyes-Figueroa, S Reyes, ...
    International Journal of Pharmaceutics 670, 125070 2025
    Citations: 1

  • 3D Printing and Bioprinting in the Battle Against Diabetes and its Chronic Complications
    I Sathisaran
    Frontiers in Bioengineering and Biotechnology 12, 1363483 2024
    Citations: 1

  • Understanding rationale behind carbamazepine cocrystallization with acids, amides and hydrazides
    I Sathisaran, SV Dalvi
    Acta Crystallographica Section A 70, C435 2017
    Citations: 1

  • Exploring cocrystallization of curcumin
    I Sathisaran, JM Skieneh, SV Dalvi, S Rohani
    Acta Crystallogr., Sect. A: Found. Adv. A 73, C1038 2017
    Citations: 1