Ionara Regina Pizzutti
@ceparc.com.br
Center for Research and Analysis of Residues and Contaminants (CEPARC)
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Cesar Vinicius Toniciolli Rigueto, Gabriel de Vasconcelos, Marieli Rosseto, Daniela Dal Castel Krein, Fernanda Oliveira, Carla Patricia Freitas, Caroline Antunes do Nascimento, Luciana Ruschel dos Santos, Raquel Aparecida Loss, Aline Dettmer,et al.
Springer Science and Business Media LLC
Cesar Vinicius Toniciolli Rigueto, Marieli Rosseto, Karolynne Sousa Gomes, Raquel Aparecida Loss, Bárbara Biduski, Christian Manera, Marcelo Godinho, Vandré Barbosa Brião, Aline Dettmer, and Ionara Regina Pizzutti
Elsevier BV
Rosselei C. da Silva, Eliane Dallegrave, and Ionara R. Pizzutti
Elsevier
Cesar Vinicius Toniciolli Rigueto, Marieli Rosseto, Ingridy Alessandretti, Daniela Dal Castel Krein, Cassandro Davi Emer, Raquel Aparecida Loss, Aline Dettmer, and Ionara Regina Pizzutti
Springer Science and Business Media LLC
Cesar Vinicius Toniciolli Rigueto, Marieli Rosseto, Raquel Aparecida Loss, Neila Silvia Pereira dos Santos Richards, Aline Dettmer, and Ionara Regina Pizzutti
FapUNIFESP (SciELO)
ABSTRACT: This research carried out a bibliometric analysis and literature review on the production of gelatin-based films for application as food packaging, addressing the main advances and limitations. The search for articles was performed in the Scopus database, and bibliometric data were obtained using the Bibliometrix tool (RStudio software). It was observed that a wide variety of compounds can be incorporated into gelatin films to overcome the limitations related to their high solubility and low mechanical properties, as well as to obtain active or smart functions. Among the most reported compounds were essential oils, pigments extracted from vegetables, and other antimicrobial agents. The most reported foods as an application matrix were meat (fish, chicken, and shrimp), milk, cheese, and minimally processed fruits. Even with promising trends, the biggest challenge for large-scale applications is to obtain easily degradable biopolymers with structural and functional stability similar to synthetic polymers. Thus, a greater focus on this theme in research may favor significant advances in the use of these packages and positively impact several of the Sustainable Development Goals, as recommended by the United Nations.
Cesar Vinicius Toniciolli Rigueto, Marieli Rosseto, Ingridy Alessandretti, Rafaela de Oliveira, Daniela Alexia Raber Wohlmuth, Juliana Ferreira Menezes, Raquel Aparecida Loss, Aline Dettmer, and Ionara Regina Pizzutti
Elsevier BV
Rosselei Caiel da Silva, Ingrid Duarte dos Santos, Júlia Paula Neu, Robson Dias Wouters, Marlos Eduardo Zorzella Fontana, Priscila Dotto Rosa Balbinot, Roger Wagner, and Ionara Regina Pizzutti
Elsevier BV
Cesar Vinicius Toniciolli Rigueto, Ingridy Alessandretti, Marieli Rosseto, Claudineia Aparecida Queli Geraldi, Raquel Aparecida Loss, Ionara Regina Pizzutti, Jeferson Steffanello Piccin, and Aline Dettmer
FapUNIFESP (SciELO)
Ingrid D. dos Santos, Marlos E.Z. Fontana, Bruna Klein, Stephanie R. Ribeiro, Andrieli Stefanello, Fabio R. Thewes, Suele F. P. Schmidt, Marina V. Copetti, Auri Brackmann, Ionara R. Pizzutti,et al.
Informa UK Limited
The objective of this study was to evaluate fungal and patulin contamination, together with its correlation with the volatile compounds (VCs), in 'Fuji Mishima' apples (up to 25% decayed) under controlled atmosphere (CA) and dynamic controlled atmosphere with respiratory quotient (DCA-RQ) of 1.3 combined with different partial pressures of carbon dioxide (0.8, 1.2, 1.6 and 2.0 pCO2). Fruits were stored under the above conditions for 8 months at 0.5 °C plus 7 days shelf life at 20 °C. Toxigenic fungi and patulin accumulation were found in apples from all treatments. Penicillium expansum was the most prevalent species. For all storage conditions, patulin concentrations were above the maximum level allowed in Brazil (50 μg kg-1) with an exception of DCA-RQ1.3 + 0.8 kPa CO2. This condition, with lower pCO2, showed the lowest patulin accumulation, below the legal limit. The CA provided the highest patulin concentration (166 μg kg-1). It was observed that fungal growth could also contribute to changes in the volatile composition. Styrene and 3-methyl-1-butanol are considered P. expansum markers in some apple cultivars and were detected in the samples. However, it was not possible to identify volatile organic compounds (VOCs) that are biomarkers from P. expansum, because there were other fungi species present in all samples. In this study, styrene, n-decanoic acid, toluene, phenol and alpha-farnesene were the compounds that showed the most positive correlation with patulin accumulation. On the other hand, a negative correlation of patulin with acids has been shown, indicating that in treatments with a higher patulin concentration there were less acidic compounds.
Rosselei Caiel da Silva, Cristine Wickert, Ionara Regina Pizzutti, and André de Kok
American Chemical Society (ACS)
Food matrices consist of many components with different physical and chemical properties that may influence instrumental robustness. The soybean contains fatty coextractives which may have a deleterious effect on the gas chromatography (GC) system. In this study, the efficiencies of PSA, C18OH, C18, silica, aluminum oxide, and Florisil, as dSPE clean-up sorbents, were evaluated by the high-performance liquid chromatography (HPLC) diode-array detector and evaporative light-scattering detector analysis. The dithiocarbamates in soybean samples are determined as CS2 using acidic hydrolysis and isooctane partitioning, followed by GC-PFPD and GC-ITD-MS analyses. The linearity of the analytical curves, the instrument limit of detection matrix effects, the trueness and precision, and the method limit of quantification (LOQ) were assessed in the validation study. Milled soybean was spiked with thiram solution at three concentration levels (corresponding to 0.05, 0.1, and 0.5 mg CS2 kg-1) for recovery determination. Silica appeared to be an effective and cheap sorbent to remove coextracted matrix components without causing any CS2 losses. Recoveries were in the range of 68-91%, with relative standard deviations ≤ 8.7%. The method LOQ was 0.05 mg CS2 kg-1, and both GC-ITD-MS and GC-PFPD systems appeared to be appropriate and complementary to determine dithiocarbamate residues in soybean extracts.
Maria da Graça Pereira Nunes, Ionara Regina Pizzutti, Auri Brackmann, Bárbara Reichert, Marlos Eduardo Zorzella Fontana, Ingrid Duarte dos Santos, Lisandra Kopp Cuti, Bárbara Daiana Jänisch, Matheus Pelizzaro Panciera, Vagner Ludwig,et al.
American Chemical Society (ACS)
Two simple and low-cost QuEChERS approaches were optimized and validated for multimycotoxin determination in grains by UPLC-MS/MS and applied to assess effectiveness of controlled atmosphere (CA) storage in preventing mycotoxin contamination. Common bean, soybean, and maize samples were stored for 6 months. CA treatments were conducted varying O2 and CO2 partial pressures, temperatures, and moisture contents of the chambers. In the validation study for common bean and maize, 8 out of 11 mycotoxins were successfully validated. For soybean, 10 out of 11 mycotoxins were validated. Aflatoxin B1 was detected in all commodities. Statistical tests suggest that storage temperature played a key role in aflatoxin B1 concentrations in common bean and soybean, but had no influence on maize. Maize was also positive for fumonisins B1 and B2. Differences in fumonisin concentrations were not significant among different treatments. Concentrations of aflatoxin B1 in some samples exceeded legislation's maximum levels. Thus, some of the CA treatments applied were effective in preventing mycotoxin contamination in common bean and soybean but were not effective for maize.
L. D. A. Dias, A. T. Itako, L. Gebler, J. B. Tolentino Júnior, I. R. Pizzutti, Marlos E. Fontana, B. Janisch and J. Niemeyer
Pesticide use generates wastewaters from its handling and from washing of spraying equipment, bringing concern about punctual discharge. Biobeds are biopurification systems for pesticide residues disposal, representing safe alternatives to avoid soil contamination. Peat that composes the original biomixture is not available all around Brazil. The aim of this work was to evaluate the efficiency of two alternative, accessible biomixtures, replacing peat, for treating phosmet (organophosphate insecticide) residues in biobeds and reducing its ecotoxicological effects. We hypothesized that the new biomixtures with pine litter (PB) or vermicompost (VB) could show the same degradation and detoxification efficiency as the standard biobed’s biomixture (SB) using peat. Small size bioreactors received 35 mg kg−1 of phosmet (Imidan®) in a laboratory-scale experiment. The pesticide degradation was monitored by chemical analysis. The decrease of ecotoxicity was determined by reproduction tests with collembolans (Folsomia candida) and enchytraeids (Enchytraeus crypticus), following ISO guidelines. Degradation curves showed that all biomixtures reached almost complete degradation of phosmet after 90 days. Collembolans were more sensitive than enchytraeid, confirming their usefulness in biomonitoring insecticide degradation. This work showed that both pine litter and vermicompost are potentially substitutes for peat in alternative biomixtures, since they were efficient in degrading the pesticide and reducing its ecotoxicity. Our results contribute for the development of newly, accessible biobeds for south Brazil, bringing the first study reports involving such biobeds capacity to degrade phosmet, which is a commonly used pesticide in this region.
Ingrid Duarte dos Santos, Ionara Regina Pizzutti, Jonatan Vinicius Dias, Marlos Eduardo Zorzella Fontana, Darliana Mello Souza, and Carmem Dickow Cardoso
Informa UK Limited
ABSTRACT The goal of this survey was to evaluate the presence and concentration as well as the co-occurrence of legislated and non-legislated mycotoxins in wheat flour samples from Brazil. A total of 200 wheat flour samples were analysed by a validated multi-mycotoxins method. DON was the mycotoxin with the highest occurrence, being present in 100% of the analysed samples and showing contamination in both years and regions (53–2905 μg kg−1). ZEN was detected in 51% (<LOQ–50 μg kg−1) of the samples, while T-2 (not legislated in Brazil) was detected in 13.5% (<LOQ–1506 μg kg−1) of all samples. Regarding co-occurrence, all samples were contaminated with two to three mycotoxins.
Mariele dos Santos, Catiucia S. Vareli, Bárbara Janisch, Ionara R. Pizzutti, Juciane Fortes, Cláudia Kaehler Sautter, and Ijoni H. Costabeber
Informa UK Limited
ABSTRACT Persistent organic pollutants are characterised by their chemical structure, environmental persistence and toxicity to human and wildlife populations. The production of these chemicals is regulated and restricted. However, they continue to be detected in the environment. In this study, the occurrence of 11 congeners of polychlorinated biphenyls (PCBs 28, 52, 77, 81, 101, 118, 126, 138, 153, 169, and 180) was investigated in 90 honey samples produced in the Brazilian state of Rio Grande do Sul. The samples were from different municipalities, production systems and floral origins. Extraction was performed using the modified QuEChERS method (Quick, Easy, Cheap, Effective, Rugged and Safe) followed by gas chromatography with micro-electron capture detector. The results showed the presence of four congeners (PCBs 28, 77, 81, 101) in 15 honey samples confirming the environmental contamination in Southern Brazil. Among the contaminated samples, no significant differences were identified regarding the production system and floral origin.
Fernanda B. Pires, Carolina B. Dolwitsch, Gustavo A. Ugalde, Bryan B. Menezes, Marlos Eduardo Z. Fontana, Roberta C. Rieffeld, Michele R. Sagrillo, Liliana Essi, Marcio Antonio Mazutti, Marcelo B. da Rosa,et al.
Informa UK Limited
Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical fluid extractions, and subsequently investigate their antioxidant potential and cyto-genotoxicity. The extract obtained by ultrasound (UAE-EtOH) was characterized by ultraperformance liquid chromatography-mass spectrometer (UPLC-MS), and the extract obtained via supercritical fluid (SFE-CO2) by gas chromatograph-mass spectrometer (GC-MS). The antioxidant potential was verified by the antiradical activity against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and the cyto-genotoxicity evaluation to test cell viability and DNA damage was performed in peripheral blood mononuclear cells (PBMC) cultures. The SFE-CO2 extract presented some fatty acids, triterpenes, tetraterpenes, tocopherols and phytosterols. The UAE-EtOH extract contained phenolic acids and flavonoids, and showed the highest antioxidant capacity. Neither extract was genotoxic or cytotoxic at the tested concentrations.
Carolina B. Dolwitsch, Fernanda B. Pires, Lucas M. Frescura, Valéria Dal Prá, Roberta C. Rieffel, Michele R. Sagrillo, Camilo A. de Carvalho, Marcio Mazutti, Ionara R. Pizzutti, and Marcelo B. da Rosa
Informa UK Limited
Hesperozygis ringens is a plant of the Lamiaceae family which is restricted to the Southern region of Brazil. It is popularly used as an insecticide, but knowledge on it is very scarce. This study aimed to determine the chemical markers of H. ringens extracts obtained via ultrasound-assisted (UAE-EtOH) and supercritical fluid (SFE-CO2) extractions. UAE-EtOH and SFE-CO2 extracts were characterised by UPLC-MS and GC-MS, respectively. Both products had their antioxidant activity, cytotoxicity and genotoxicity evaluated. Twelve compounds were found in the UAE-EtOH extract, including phenolic acids and flavonoids; the SFE-CO2 extract contained terpenes and phytosterols. The UAE-EtOH extract showed a greater antioxidant activity. Neither extract presented cytotoxicity or genotoxicity against human mononuclear blood cells.
G. Concenço, R. Vivian, F. S. Ikeda, I. R. Pizzutti, G.M.E. Vela, V.L. Ferracini, L. Nora, G. Ceccon, and F.I.G.R. Concenço
Cambridge University Press (CUP)
AbstractCowpea bean (Vigna unguiculata) is usually submitted to pre-harvest burndown with herbicides aiming to an early harvest. We aimed to quantify the residues of paraquat, diquat, glufosinate-ammonium and glyphosate in cowpea grains subjected to two timings of pre-harvest burndown. Three identical experiments were installed in Dourados (Lat. 22° S), Sinop (Lat. 11° S) and Teresina (Lat. 05° S), Brazil, comprising the pre-harvest burndown of cowpea bean with these herbicides, applied either with 50 or 75% of the pods dry and ready for harvest. As hotter and drier the location as slower the herbicide dissipation. Glyphosate should not be used for pre-harvest burndown of cowpea. Herbicide residues were above the maximum levels stated by the Brazilian legislation when applied with 50% dry pods. Burndown herbicides aiming to speed harvest should be applied with at least 75% dry pods in cowpea to ensure safe herbicide residue levels in grains.
Gustavo D. Quatrin, Ionara R. Pizzutti, Luciano Gebler, Jonatan V. Dias, and Carmem D. Cardoso
Elsevier BV
A quick and efficient method was optimized and validated to determine chlorpyrifos in biobeds using ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS). Chlorpyrifos was extracted from the matrix with 30 mL of a mixture of acetone, phosphoric acid and water 98:1:1 (v/v/v). After homogenization, centrifugation and filtration, 125 µL of the extract was evaporated and reconstituted in 5 mL of methanol acidified with 0.1% acetic acid. Validation was performed by studying analytical curve linearity (r2), estimated instrument and method limits of detection and limits of quantification (LODi, LODm, LOQi and LOQm, respectively), accuracy, precision (expressed as relative standard deviation, RSD), and matrix effect. Accuracy and precision were determined from the amount of pesticide recovered from biobed blank samples (i.e. without pesticide residue) spiked with chlorpyrifos at three different concentrations (2, 10 and 50 mg kg-1), with seven replicates at each concentration. For all three concentrations studied, the average recovery values obtained were between 96 and 115% with RSD values lower than 20%. The validated LOQ obtained was 2 mg kg-1 (from recovery studies) and the matrix effect observed was lower than ±20%, which demonstrated that there was neither considerable suppression nor enhancement of the analyte signal. The biobed system efficiently degraded chlorpyrifos in both 1) simulation of accidental spillage and 2) application of diluted pesticide solution. In the latter case, all the values obtained at the final sampling time (14 months) were below the validated LOQm.
Patrícia Diniz Andrade, Jonatan Vinicius Dias, Darliana Mello Souza, Alessandra Page Brito, Gerda van Donkersgoed, Ionara Regina Pizzutti, and Eloisa Dutra Caldas
Elsevier BV
A probabilistic dietary risk assessment on mycotoxins was conducted using the Monte Carlo Risk Assessment software, with consumption data from the 2008/2009 Brazilian Household Budget Survey for individuals who were at least 10 years old and occurrence data for 646 samples of rice, maize, wheat, and their products, collected in the Federal District and in the state of Rio Grande do Sul, Brazil. Processing factors were estimated and applied to concentration data. Chronic exposure was estimated for fumonisins (free and bound/hidden), deoxynivalenol (DON) (including the acetylated forms) and zearalenone (ZON) (including alfa-zearalenol) and acute exposure to DON. For the general population, the chronic exposure exceeded the safe exposure levels at the 95P for DON and at the 99P for fumonisins. Additionally, safe level exceedance occurred at the 97.5P for fumonisins and at the 95P for DON for teenagers, as well as at the 99P for fumonisins for women of child-bearing-age. No exceedances were found for chronic exposure to ZON and acute exposure to DON. Maize couscous contributed most of the total fumonisins (91%) and ZON intakes (∼40%) and bread to total intake of DON (∼30%). Further studies should be conducted with updated Brazilian consumption data, which should include information for individuals aged less than 10 years old.
Bárbara Reichert, Maria G Pereira Nunes, Ionara R Pizzutti, Ijoni H Costabeber, Marlos Z Fontana, Bárbara D Jänich, Matheus P Panciera, Dênia Arbusti, Carmem D Cardoso, and Josiane F Chim
Wiley
BACKGROUND
Common bean is a staple food in Latin America and Africa, however, studies about contamination of common bean with pesticides are rarely reported. So, the goals of this study were to validate a multiresidue method and apply it in monitoring of pesticides in common beans. Extraction was performed applying QuEChERS approach to ground samples. C18 and PSA were used for clean-up and a solvent exchange step was performed before injection into the GC-MS/MS. Method validation was done analyzing blank samples spiked at 20, 30, 50 and 100 μg kg-1 (n = 5). Linearity and linear range were assessed by the analysis of standard solutions at concentrations of 5, 15, 25, 50, 75, 100 and 150 μg L-1 .
RESULTS
The method was successfully validated for 91 (64.1%) of the 142 compounds studied (139 pesticides and 3 degradation products). LOQs were equal to 20, 30, 50 and 100 μg kg-1 for 18 (12.7%), 17 (12.0%), 21 (14.8%) and 35 (24.6%) compounds, respectively. Fifteen (10.6%) compounds were not detected at any level and 36 (25.4%) did not fulfill requirements for quantitative method. Sixteen common bean samples of South of Brazil were analyzed. Two samples were positive, one for tebuconzole and a second for picoxystrobin, permethrin and cyproconazole. Cyproconazole is not allowed for the crop, consisting of a violation.
CONCLUSION
As demonstrated the validated approach is suitable for pesticide residues determination in common bean. Results of the sample analysis show that the control of pesticide residues in common bean is necessary to ensure food safety. This article is protected by copyright. All rights reserved.
Catiucia S. Vareli, Ionara R. Pizzutti, Luciano Gebler, Carmem D. Cardoso, Marlos E. Z. Fontana, Bárbara Reichert, and Bárbara D. Jänisch
Royal Society of Chemistry (RSC)
An optimized version of the Dutch mini-Luke method has been validated for determination of chlorothalonil, chlorpyrifos-ethyl, fenitrothion, methidathion, phosmet, tebuconazole and tetrahydrophthalimide in biobeds.
Jonatan V. Dias, Maria da Graça P. Nunes, Ionara R. Pizzutti, Bárbara Reichert, Aliar A. Jung, and Carmem D. Cardoso
Elsevier BV
A simple and rapid method for simultaneous determination of pesticides and mycotoxins in red wine is presented. Sample preparation approach, called direct injection, consists of a sequence of only three steps: centrifugation, dilution and filtration. The analysis of extracts were performed by UPLC-MS/MS for determination of pesticides and mycotoxins. The method was assessed for linearity, limits of detection and quantification, matrix effects, selectivity, accuracy and precision. For recovery experiments, mycotoxins were divided in two groups according to their sensitivity in the UPLC-MS/MS system. More than 80% of the mycotoxins were reliably quantified at the lowest spike level studied (1 μg kg-1 for group 1 and 50 μg kg-1 for group 2). From the 185 evaluated pesticides, 144 showed acceptable results at 10 μg kg-1, the lowest spiked level. Matrix effects were, in most of the cases, negative, and that was observed for both pesticides and mycotoxins.
Jonatan V. Dias, Rosselei C. da Silva, Ionara R. Pizzutti, Ingrid D. dos Santos, Micheli Dassi, and Carmem D. Cardoso
Elsevier BV
Abstract In this work, a novel single method for patulin determination in apple and apple juice employing liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is presented. Samples were extracted employing ethyl acetate acidified with acetic acid followed by sodium sulfate addition. The extracts were submitted to dispersive solid phase clean-up employing sodium sulfate and silica gel. The method was validated assessing linearity and linearity range, limits of detection and quantification, matrix effect, precision (RSD%) and accuracy (recovery from samples spiked at 25, 50 and 100 μg kg−1). The method showed to be suitable for patulin determination as the recovery ranged from 76 to 84% for apple and from 102 to 108% for apple juice, in both cases with RSD lower than 15%. Matrix effect was also evaluated and showed to be less intense for apple juice (−22%) than for apples (−42%). The validated method was applied to 62 samples (apple and apple juice) collected in supermarkets of southern Brazil, during the years 2016 and 2017. Apple samples showed contamination ranging from
Andressa Lanza, Rosselei Caiél da Silva, Ingrid Duarte dos Santos, Ionara Regina Pizzutti, Karine Cence, Rogério Luis Cansian, Jamile Zeni, and Eunice Valduga
Springer Science and Business Media LLC
The aim of this study was to evaluate the mycotoxicological quality of wheat flours used by bakeries from the North Region in Rio Grande do Sul state, Brazil, regarding the presence of mycotoxins. On collecting type-1 refined wheat flour, a conglomerate sampling from 13 cities and 3 bakeries per city (n = 39), selected from the defined region was performed. The mycotoxins analysis was using QuEChERS method and UPLC–MS/MS analysis. As a result, 100% of samples presented contamination by DON, with concentrations ranging from 76.7 to 3630.2 µg kg−1 and ZON was found in one sample (26.7 µg kg−1), which represented 2.6% of the analyzed wheat flours. Other mycotoxins (AFB1, AFB2, AFG1, AFG2, DAS, HT-2 toxin, OTA, FB1 and FB2) were not detected in the analyzed samples.Graphical abstract
Ingrid D. dos Santos, Ionara R. Pizzutti, Jonatan V. Dias, Marlos E.Z. Fontana, Auri Brackmann, Rogério O. Anese, Fabio R. Thewes, Leandro N. Marques, and Carmem D. Cardoso
Elsevier BV
The goal of this study was to evaluate patulin contamination in 'Galaxy' and 'Fuji Kiku' apples subjected to controlled atmosphere (CA) and dynamic controlled atmosphere (DCA) conditions. Experiments were performed and fruit were stored for nine months under refrigeration plus 7 days shelf life at 20 °C. CA and DCA were not effective in preventing patulin production in either 'Galaxy' or 'Fuji Kiku' apples. Healthy fruit were not contaminated with patulin, even when stored together with decayed apples. For 'Galaxy' apples, application of 1-methylcyclopropene increased the percentage of fruit with decay and patulin contamination. Patulin concentrations were above the maximum limit (50 μg kg-1) established in the Brazilian legislation, meaning the use of CA and DCA conditions were not advantageous in preventing patulin accumulation. In 'Fuji Kiku' apples, there was no significant difference in patulin concentration among CA, DCA-CF and DCA-RQ 1.3 treatments, and all were below the maximum.