Functional and evolutionary diversification of luciferase genes in Metridia lucens Boeck 1865 Luís B. Gabín-García, Carolina Bartolomé, Pablo Iglesias, Ánxela M. Estévez-Salguero, Laura Rodríguez de la Fuente, Xulio Maside, Jose A. Costoya Scientific Reports, 2026 Abstract Bioluminescent organisms have developed extraordinary adaptations to produce light using a luciferin-luciferase reaction, fulfilling various ecological functions such as predator evasion, prey attraction, and intraspecies communication. Although the earliest record in the marine environment dates back some 540 million years, the evolutionary origins of this phenomenon remain largely unknown in most species. In Metridinidae copepods, light production capability is facilitated by a luciferase gene duplication. This study focuses on characterizing the luciferase genes of Metridia lucens, a copepod widely distributed throughout global oceans, excluding the high Arctic. Despite being the first species described in this genus, the genomic sequences of its luciferase genes remained unknown prior to this investigation. Here, using an integrated approach combining molecular cloning and high-throughput sequencing, we isolated and characterized M. lucens luciferase genes. Our results revealed an unexpectedly high genetic diversity both within and between specimens, consistent with the presence of an extended gene family in this copepod’s genome. We identified three distinct luciferase gene lineages, each represented by several copies, expanding our knowledge of marine bioluminescence evolution.
Neural stem cells and glioblastoma stem cells: Redefining concepts Víctor M. Arce, Lara González-Rendo, Laura Porres-Ventín, Valentina González-Álvarez, Sabela Caamaño-Teixeira, Cristina Almengló, Rosa Señarís, José A. Costoya Critical Reviews in Oncology Hematology, 2026 Stem cells (SCs) represent a distinctive population of undifferentiated cells with the extraordinary ability to self-renew and differentiate into multiple cell types. Owing to this, SCs play a crucial role in maintaining tissue homeostasis, providing a source for the replacement of cell losses due to normal wear and tear. In addition, SCs display an unquestioned therapeutic potential, which has resulted in the development of several cell therapies for the treatment of different types of diseases. However, despite their remarkable potential, the therapeutic use of SCs must still face several challenges, which include ethical, legal and technical issues. Ethical and legal concerns are mainly related to the use of SCs obtained from human embryos, while technical problems mostly arise from the difficulty of appropriately directing the differentiation of the SCs to meet the tissue´s needs and the occurrence of events such as immune rejections. In addition, the safety of SC-based therapies is also under debate. Although they may offer a useful and harmless treatment for many pathologies, including some incurable and/or life-threatening diseases, a potential risk of tumorigenicity may also exist in some cases. Accumulating evidence also implicates SCs as the origin of, at least, some types of cancer. This is the case of glioblastoma (GBM), the most prevalent glioma type in adults, whose origin has been related to postnatal neural stem cells (NSCs), mainly located in the subventricular zone (SVZ) and the dentate gyrus in the hippocampus. It has been proposed that these NSCs may give rise to glioblastoma stem cells (GSCs), which through complex interactions with the tumor microenvironment exert a crucial effect on tumor growth and development. • Cancer stem cells are initially originated from resident stem cells, although this does not exclude the possibility that non-stem cancer cells may de-differentiate into cancer stem cells. • Glioma stem cells mimic normal stem cell behavior escaping from therapies targeting dividing cells. • Multiple mechanisms are involved in the crosstalk between glioblastoma and tumor microenvironment, including local regulation and distal hormonal signals and inflammatory mediators. • Cellular heterogeneity in glioblastoma is mainly due to the existence of a unique population of cancer stem cells, which are known as glioma stem cells.
AI is a viable alternative to high throughput screening: a 318-target study , Izhar Wallach, Denzil Bernard, Kong Nguyen, Gregory Ho, Adrian Morrison, Adrian Stecula, Andreana Rosnik, Ann Marie O’Sullivan, Aram Davtyan, Ben Samudio, Bill Thomas, Brad Worley, Brittany Butler, Christian Laggner, Desiree Thayer, Ehsan Moharreri, Greg Friedland, Ha Truong, Henry van den Bedem, Ho Leung Ng, Kate Stafford, Krishna Sarangapani, Kyle Giesler, Lien Ngo, Michael Mysinger, Mostafa Ahmed, Nicholas J. Anthis, Niel Henriksen, Pawel Gniewek, Sam Eckert, Saulo de Oliveira, Shabbir Suterwala, Srimukh Veccham Krishna PrasadPrasad, Stefani Shek, Stephanie Contreras, Stephanie Hare, Teresa Palazzo, Terrence E. O’Brien, Tessa Van Grack, Tiffany Williams, Ting-Rong Chern, Victor Kenyon, Andreia H. Lee, Andrew B. Cann, Bastiaan Bergman, Brandon M. Anderson, Bryan D. Cox, Jeffrey M. Warrington, Jon M. Sorenson, Joshua M. Goldenberg, Matthew A. Young, Nicholas DeHaan, Ryan P. Pemberton, Stefan Schroedl, Tigran M. Abramyan, Tushita Gupta, Venkatesh Mysore, Adam G. Presser, Adolfo A. Ferrando, Adriano D. Andricopulo, Agnidipta Ghosh, Aicha Gharbi Ayachi, Aisha Mushtaq, Ala M. Shaqra, Alan Kie Leong Toh, Alan V. Smrcka, Alberto Ciccia, Aldo Sena de Oliveira, Aleksandr Sverzhinsky, Alessandra Mara de Sousa, Alexander I. Agoulnik, Alexander Kushnir, Alexander N. Freiberg, Alexander V. Statsyuk, Alexandre R. Gingras, Alexei Degterev, Alexey Tomilov, Alice Vrielink, Alisa A. Garaeva, Amanda Bryant-Friedrich, Amedeo Caflisch, Amit K. Patel, Amith Vikram Rangarajan, An Matheeussen, Andrea Battistoni, Andrea Caporali, Andrea Chini, Andrea Ilari, Andrea Mattevi, Andrea Talbot Foote, Andrea Trabocchi, Andreas Stahl, Andrew B. Herr, Andrew Berti, Andrew Freywald, Andrew G. Reidenbach, Andrew Lam, Andrew R. Cuddihy, Andrew White, Angelo Taglialatela, Anil K. Ojha, Ann M. Cathcart, Anna A. L. Motyl, Anna Borowska, Anna D’Antuono, Anna K. H. Hirsch, Anna Maria Porcelli, Anna Minakova, Anna Montanaro, Anna Müller, Annarita Fiorillo, Anniina Virtanen, Anthony J. O’Donoghue, Antonio Del Rio Flores, Antonio E. Garmendia, Antonio Pineda-Lucena, Antonito T. Panganiban, Ariela Samantha, Arnab K. Chatterjee, Arthur L. Haas, Ashleigh S. Paparella, Ashley L. St. John, Ashutosh Prince, Assmaa ElSheikh, Athena Marie Apfel, Audrey Colomba, Austin O’Dea, Bakary N’tji Diallo, Beatriz Murta Rezende Moraes Ribeiro, Ben A. Bailey-Elkin, Benjamin L. Edelman, Benjamin Liou, Benjamin Perry, Benjamin Soon Kai Chua, Benjámin Kováts, Bernhard Englinger, Bijina Balakrishnan, Bin Gong, Bogos Agianian, Brandon Pressly, Brenda P. Medellin Salas, Brendan M. Duggan, Brian V. Geisbrecht, Brian W. Dymock, Brianna C. Morten, Bruce D. Hammock, Bruno Eduardo Fernandes Mota, Bryan C. Dickinson, Cameron Fraser, Camille Lempicki, Carl D. Novina, Carles Torner, Carlo Ballatore, Carlotta Bon, Carly J. Chapman, Carrie L. Partch, Catherine T. Chaton, Chang Huang, Chao-Yie Yang, Charlene M. Kahler, Charles Karan, Charles Keller, Chelsea L. Dieck, Chen Huimei, Chen Liu, Cheryl Peltier, Chinmay Kumar Mantri, Chinyere Maat Kemet, Christa E. Müller, Christian Weber, Christina M. Zeina, Christine S. Muli, Christophe Morisseau, Cigdem Alkan, Clara Reglero, Cody A. Loy, Cornelia M. Wilson, Courtney Myhr, Cristina Arrigoni, Cristina Paulino, César Santiago, Dahai Luo, Damon J. Tumes, Daniel A. Keedy, Daniel A. Lawrence, Daniel Chen, Danny Manor, Darci J. Trader, David A. Hildeman, David H. Drewry, David J. Dowling, David J. Hosfield, David M. Smith, David Moreira, David P. Siderovski, David Shum, David T. Krist, David W. H. Riches, Davide Maria Ferraris, Deborah H. Anderson, Deirdre R. Coombe, Derek S. Welsbie, Di Hu, Diana Ortiz, Dina Alramadhani, Dingqiang Zhang, Dipayan Chaudhuri, Dirk J. Slotboom, Donald R. Ronning, Donghan Lee, Dorian Dirksen, Douglas A. Shoue, Douglas William Zochodne, Durga Krishnamurthy, Dustin Duncan, Dylan M. Glubb, Edoardo Luigi Maria Gelardi, Edward C. Hsiao, Edward G. Lynn, Elany Barbosa Silva, Elena Aguilera, Elena Lenci, Elena Theres Abraham, Eleonora Lama, Eleonora Mameli, Elisa Leung, Emily M. Christensen, Emily R. Mason, Enrico Petretto, Ephraim F. Trakhtenberg, Eric J. Rubin, Erick Strauss, Erik W. Thompson, Erika Cione, Erika Mathes Lisabeth, Erkang Fan, Erna Geessien Kroon, Eunji Jo, Eva M. García-Cuesta, Evgenia Glukhov, Evripidis Gavathiotis, Fang Yu, Fei Xiang, Fenfei Leng, Feng Wang, Filippo Ingoglia, Focco van den Akker, Francesco Borriello, Franco J. Vizeacoumar, Frank Luh, Frederick S. Buckner, Frederick S. Vizeacoumar, Fredj Ben Bdira, Fredrik Svensson, G. Marcela Rodriguez, Gabriella Bognár, Gaia Lembo, Gang Zhang, Garrett Dempsey, Gary Eitzen, Gaétan Mayer, Geoffrey L. Greene, George A. Garcia, Gergely L. Lukacs, Gergely Prikler, Gian Carlo G. Parico, Gianni Colotti, Gilles De Keulenaer, Gino Cortopassi, Giovanni Roti, Giulia Girolimetti, Giuseppe Fiermonte, Giuseppe Gasparre, Giuseppe Leuzzi, Gopal Dahal, Gracjan Michlewski, Graeme L. Conn, Grant David Stuchbury, Gregory R. Bowman, Grzegorz Maria Popowicz, Guido Veit, Guilherme Eduardo de Souza, Gustav Akk, Guy Caljon, Guzmán Alvarez, Gwennan Rucinski, Gyeongeun Lee, Gökhan Cildir, Hai Li, Hairol E. Breton, Hamed Jafar-Nejad, Han Zhou, Hannah P. Moore, Hannah Tilford, Haynes Yuan, Heesung Shim, Heike Wulff, Heinrich Hoppe, Helena Chaytow, Heng-Keat Tam, Holly Van Remmen, Hongyang Xu, Hosana Maria Debonsi, Howard B. Lieberman, Hoyoung Jung, Hua-Ying Fan, Hui Feng, Hui Zhou, Hyeong Jun Kim, Iain R. Greig, Ileana Caliandro, Ileana Corvo, Imanol Arozarena, Imran N. Mungrue, Ingrid M. Verhamme, Insaf Ahmed Qureshi, Irina Lotsaris, Isin Cakir, J. Jefferson P. Perry, Jacek Kwiatkowski, Jacob Boorman, Jacob Ferreira, Jacob Fries, Jadel Müller Kratz, Jaden Miner, Jair L. Siqueira-Neto, James G. Granneman, James Ng, James Shorter, Jan Hendrik Voss, Jan M. Gebauer, Janelle Chuah, Jarrod J. Mousa, Jason T. Maynes, Jay D. Evans, Jeffrey Dickhout, Jeffrey P. MacKeigan, Jennifer N. Jossart, Jia Zhou, Jiabei Lin, Jiake Xu, Jianghai Wang, Jiaqi Zhu, Jiayu Liao, Jingyi Xu, Jinshi Zhao, Jiusheng Lin, Jiyoun Lee, Joana Reis, Joerg Stetefeld, John B. Bruning, John Burt Bruning, John G. Coles, John J. Tanner, John M. Pascal, Jonathan So, Jordan L. Pederick, Jose A. Costoya, Joseph B. Rayman, Joseph J. Maciag, Joshua Alexander Nasburg, Joshua J. Gruber, Joshua M. Finkelstein, Joshua Watkins, José Miguel Rodríguez-Frade, Juan Antonio Sanchez Arias, Juan José Lasarte, Julen Oyarzabal, Julian Milosavljevic, Julie Cools, Julien Lescar, Julijus Bogomolovas, Jun Wang, Jung-Min Kee, Jung-Min Kee, Junzhuo Liao, Jyothi C. Sistla, Jônatas Santos Abrahão, Kamakshi Sishtla, Karol R. Francisco, Kasper B. Hansen, Kathleen A. Molyneaux, Kathryn A. Cunningham, Katie R. Martin, Kavita Gadar, Kayode K. Ojo, Keith S. Wong, Kelly L. Wentworth, Kent Lai, Kevin A. Lobb, Kevin M. Hopkins, Keykavous Parang, Khaled Machaca, Kien Pham, Kim Ghilarducci, Kim S. Sugamori, Kirk James McManus, Kirsikka Musta, Kiterie M. E. Faller, Kiyo Nagamori, Konrad J. Mostert, Konstantin V. Korotkov, Koting Liu, Kristiana S. Smith, Kristopher Sarosiek, Kyle H. Rohde, Kyu Kwang Kim, Kyung Hyeon Lee, Lajos Pusztai, Lari Lehtiö, Larisa M. Haupt, Leah E. Cowen, Lee J. Byrne, Leila Su, Leon Wert-Lamas, Leonor Puchades-Carrasco, Lifeng Chen, Linda H. Malkas, Ling Zhuo, Lizbeth Hedstrom, Lizbeth Hedstrom, Loren D. Walensky, Lorenzo Antonelli, Luisa Iommarini, Luke Whitesell, Lía M. Randall, M. Dahmani Fathallah, Maira Harume Nagai, Mairi Louise Kilkenny, Manu Ben-Johny, Marc P. Lussier, Marc P. Windisch, Marco Lolicato, Marco Lucio Lolli, Margot Vleminckx, Maria Cristina Caroleo, Maria J. Macias, Marilia Valli, Marim M. Barghash, Mario Mellado, Mark A. Tye, Mark A. Wilson, Mark Hannink, Mark R. Ashton, Mark Vincent C.dela Cerna, Marta Giorgis, Martin K. Safo, Martin St. Maurice, Mary Ann McDowell, Marzia Pasquali, Masfique Mehedi, Mateus Sá Magalhães Serafim, Matthew B. Soellner, Matthew G. Alteen, Matthew M. Champion, Maxim Skorodinsky, Megan L. O’Mara, Mel Bedi, Menico Rizzi, Michael Levin, Michael Mowat, Michael R. Jackson, Mikell Paige, Minnatallah Al-Yozbaki, Miriam A. Giardini, Mirko M. Maksimainen, Monica De Luise, Muhammad Saddam Hussain, Myron Christodoulides, Natalia Stec, Natalia Zelinskaya, Natascha Van Pelt, Nathan M. Merrill, Nathanael Singh, Neeltje A. Kootstra, Neeraj Singh, Neha S. Gandhi, Nei-Li Chan, Nguyen Mai Trinh, Nicholas O. Schneider, Nick Matovic, Nicola Horstmann, Nicola Longo, Nikhil Bharambe, Nirvan Rouzbeh, Niusha Mahmoodi, Njabulo Joyfull Gumede, Noelle C. Anastasio, Noureddine Ben Khalaf, Obdulia Rabal, Olga Kandror, Olivier Escaffre, Olli Silvennoinen, Ozlem Tastan Bishop, Pablo Iglesias, Pablo Sobrado, Patrick Chuong, Patrick O’Connell, Pau Martin-Malpartida, Paul Mellor, Paul V. Fish, Paulo Otávio Lourenço Moreira, Pei Zhou, Pengda Liu, Pengda Liu, Pengpeng Wu, Percy Agogo-Mawuli, Peter L. Jones, Peter Ngoi, Peter Toogood, Philbert Ip, Philipp von Hundelshausen, Pil H. Lee, Rachael B. Rowswell-Turner, Rafael Balaña-Fouce, Rafael Eduardo Oliveira Rocha, Rafael V. C. Guido, Rafaela Salgado Ferreira, Rajendra K. Agrawal, Rajesh K. Harijan, Rajesh Ramachandran, Rajkumar Verma, Rakesh K. Singh, Rakesh Kumar Tiwari, Ralph Mazitschek, Rama K. Koppisetti, Remus T. Dame, Renée N. Douville, Richard C. Austin, Richard E. Taylor, Richard G. Moore, Richard H. Ebright, Richard M. Angell, Riqiang Yan, Rishabh Kejriwal, Robert A. Batey, Robert Blelloch, Robert J. Vandenberg, Robert J. Hickey, Robert J. Kelm, Robert J. Lake, Robert K. Bradley, Robert M. Blumenthal, Roberto Solano, Robin Matthias Gierse, Ronald E. Viola, Ronan R. McCarthy, Rosa Maria Reguera, Ruben Vazquez Uribe, Rubens Lima do Monte-Neto, Ruggiero Gorgoglione, Ryan T. Cullinane, Sachin Katyal, Sakib Hossain, Sameer Phadke, Samuel A. Shelburne, Sandra E. Geden, Sandra Johannsen, Sarah Wazir, Scott Legare, Scott M. Landfear, Senthil K. Radhakrishnan, Serena Ammendola, Sergei Dzhumaev, Seung-Yong Seo, Shan Li, Shan Zhou, Shaoyou Chu, Shefali Chauhan, Shinsaku Maruta, Shireen R. Ashkar, Show-Ling Shyng, Silvestro G. Conticello, Silvia Buroni, Silvia Garavaglia, Simon J. White, Siran Zhu, Sofiya Tsimbalyuk, Somaia Haque Chadni, Soo Young Byun, Soonju Park, Sophia Q. Xu, Sourav Banerjee, Stefan Zahler, Stefano Espinoza, Stefano Gustincich, Stefano Sainas, Stephanie L. Celano, Stephen J. Capuzzi, Stephen N. Waggoner, Steve Poirier, Steven H. Olson, Steven O. Marx, Steven R. Van Doren, Suryakala Sarilla, Susann M. Brady-Kalnay, Sydney Dallman, Syeda Maryam Azeem, Tadahisa Teramoto, Tamar Mehlman, Tarryn Swart, Tatjana Abaffy, Tatos Akopian, Teemu Haikarainen, Teresa Lozano Moreda, Tetsuro Ikegami, Thaiz Rodrigues Teixeira, Thilina D. Jayasinghe, Thomas H. Gillingwater, Thomas Kampourakis, Timothy I. Richardson, Timothy J. Herdendorf, Timothy J. Kotzé, Timothy R. O’Meara, Timothy W. Corson, Tobias Hermle, Tomisin Happy Ogunwa, Tong Lan, Tong Su, Toshihiro Banjo, Tracy A. O’Mara, Tristan Chou, Tsui-Fen Chou, Ulrich Baumann, Umesh R. Desai, Vaibhav P. Pai, Van Chi Thai, Vasudha Tandon, Versha Banerji, Victoria L. Robinson, Vignesh Gunasekharan, Vigneshwaran Namasivayam, Vincent F. M. Segers, Vincent Maranda, Vincenza Dolce, Vinícius Gonçalves Maltarollo, Viola Camilla Scoffone, Virgil A. Woods, Virginia Paola Ronchi, Vuong Van Hung Le, W. Brent Clayton, W. Todd Lowther, Walid A. Houry, Wei Li, Weiping Tang, Wenjun Zhang, Wesley C. Van Voorhis, William A. Donaldson, William C. Hahn, William G. Kerr, William H. Gerwick, William J. Bradshaw, Wuen Ee Foong, Xavier Blanchet, Xiaoyang Wu, Xin Lu, Xin Qi, Xin Xu, Xinfang Yu, Xingping Qin, Xingyou Wang, Xinrui Yuan, Xu Zhang, Yan Jessie Zhang, Yanmei Hu, Yasser Ali Aldhamen, Yicheng Chen, Yihe Li, Ying Sun, Yini Zhu, Yogesh K. Gupta, Yolanda Pérez-Pertejo, Yong Li, Young Tang, Yuan He, Yuk-Ching Tse-Dinh, Yulia A. Sidorova, Yun Yen, Yunlong Li, Zachary J. Frangos, Zara Chung, Zhengchen Su, Zhenghe Wang, Zhiguo Zhang, Zhongle Liu, Zintis Inde, Zoraima Artía, Abraham Heifets Scientific Reports, 2024 High throughput screening (HTS) is routinely used to identify bioactive small molecules. This requires physical compounds, which limits coverage of accessible chemical space. Computational approaches combined with vast on-demand chemical libraries can access far greater chemical space, provided that the predictive accuracy is sufficient to identify useful molecules. Through the largest and most diverse virtual HTS campaign reported to date, comprising 318 individual projects, we demonstrate that our AtomNet® convolutional neural network successfully finds novel hits across every major therapeutic area and protein class. We address historical limitations of computational screening by demonstrating success for target proteins without known binders, high-quality X-ray crystal structures, or manual cherry-picking of compounds. We show that the molecules selected by the AtomNet® model are novel drug-like scaffolds rather than minor modifications to known bioactive compounds. Our empirical results suggest that computational methods can substantially replace HTS as the first step of small-molecule drug discovery.
Correction to: AI is a viable alternative to high throughput screening: a 318-target study (Scientific Reports, (2024), 14, 1, (7526), 10.1038/s41598-024-54655-z) , Izhar Wallach, Denzil Bernard, Kong Nguyen, Gregory Ho, Adrian Morrison, Adrian Stecula, Andreana Rosnik, Ann Marie O’Sullivan, Aram Davtyan, Ben Samudio, Bill Thomas, Brad Worley, Brittany Butler, Christian Laggner, Desiree Thayer, Ehsan Moharreri, Greg Friedland, Ha Truong, Henry van den Bedem, Ho Leung Ng, Kate Stafford, Krishna Sarangapani, Kyle Giesler, Lien Ngo, Michael Mysinger, Mostafa Ahmed, Nicholas J. Anthis, Niel Henriksen, Pawel Gniewek, Sam Eckert, Saulo de Oliveira, Shabbir Suterwala, Srimukh Veccham Krishna PrasadPrasad, Stefani Shek, Stephanie Contreras, Stephanie Hare, Teresa Palazzo, Terrence E. O’Brien, Tessa Van Grack, Tiffany Williams, Ting-Rong Chern, Victor Kenyon, Andreia H. Lee, Andrew B. Cann, Bastiaan Bergman, Brandon M. Anderson, Bryan D. Cox, Jeffrey M. Warrington, Jon M. Sorenson, Joshua M. Goldenberg, Matthew A. Young, Nicholas DeHaan, Ryan P. Pemberton, Stefan Schroedl, Tigran M. Abramyan, Tushita Gupta, Venkatesh Mysore, Adam G. Presser, Adolfo A. Ferrando, Adriano D. Andricopulo, Agnidipta Ghosh, Aicha Gharbi Ayachi, Aisha Mushtaq, Ala M. Shaqra, Alan Kie Leong Toh, Alan V. Smrcka, Alberto Ciccia, Aldo Sena de Oliveira, Aleksandr Sverzhinsky, Alessandra Mara de Sousa, Alexander I. Agoulnik, Alexander Kushnir, Alexander N. Freiberg, Alexander V. Statsyuk, Alexandre R. Gingras, Alexei Degterev, Alexey Tomilov, Alice Vrielink, Alisa A. Garaeva, Amanda Bryant-Friedrich, Amedeo Caflisch, Amit K. Patel, Amith Vikram Rangarajan, An Matheeussen, Andrea Battistoni, Andrea Caporali, Andrea Chini, Andrea Ilari, Andrea Mattevi, Andrea Talbot Foote, Andrea Trabocchi, Andreas Stahl, Andrew B. Herr, Andrew Berti, Andrew Freywald, Andrew G. Reidenbach, Andrew Lam, Andrew R. Cuddihy, Andrew White, Angelo Taglialatela, Anil K. Ojha, Ann M. Cathcart, Anna A. L. Motyl, Anna Borowska, Anna D’Antuono, Anna K. H. Hirsch, Anna Maria Porcelli, Anna Minakova, Anna Montanaro, Anna Müller, Annarita Fiorillo, Anniina Virtanen, Anthony J. O’Donoghue, Antonio Del Rio Flores, Antonio E. Garmendia, Antonio Pineda-Lucena, Antonito T. Panganiban, Ariela Samantha, Arnab K. Chatterjee, Arthur L. Haas, Ashleigh S. Paparella, Ashley L. St. John, Ashutosh Prince, Assmaa ElSheikh, Athena Marie Apfel, Audrey Colomba, Austin O’Dea, Bakary N’tji Diallo, Beatriz Murta Rezende Moraes Ribeiro, Ben A. Bailey-Elkin, Benjamin L. Edelman, Benjamin Liou, Benjamin Perry, Benjamin Soon Kai Chua, Benjámin Kováts, Bernhard Englinger, Bijina Balakrishnan, Bin Gong, Bogos Agianian, Brandon Pressly, Brenda P. Medellin Salas, Brendan M. Duggan, Brian V. Geisbrecht, Brian W. Dymock, Brianna C. Morten, Bruce D. Hammock, Bruno Eduardo Fernandes Mota, Bryan C. Dickinson, Cameron Fraser, Camille Lempicki, Carl D. Novina, Carles Torner, Carlo Ballatore, Carlotta Bon, Carly J. Chapman, Carrie L. Partch, Catherine T. Chaton, Chang Huang, Chao-Yie Yang, Charlene M. Kahler, Charles Karan, Charles Keller, Chelsea L. Dieck, Chen Huimei, Chen Liu, Cheryl Peltier, Chinmay Kumar Mantri, Chinyere Maat Kemet, Christa E. Müller, Christian Weber, Christina M. Zeina, Christine S. Muli, Christophe Morisseau, Cigdem Alkan, Clara Reglero, Cody A. Loy, Cornelia M. Wilson, Courtney Myhr, Cristina Arrigoni, Cristina Paulino, César Santiago, Dahai Luo, Damon J. Tumes, Daniel A. Keedy, Daniel A. Lawrence, Daniel Chen, Danny Manor, Darci J. Trader, David A. Hildeman, David H. Drewry, David J. Dowling, David J. Hosfield, David M. Smith, David Moreira, David P. Siderovski, David Shum, David T. Krist, David W. H. Riches, Davide Maria Ferraris, Deborah H. Anderson, Deirdre R. Coombe, Derek S. Welsbie, Di Hu, Diana Ortiz, Dina Alramadhani, Dingqiang Zhang, Dipayan Chaudhuri, Dirk J. Slotboom, Donald R. Ronning, Donghan Lee, Dorian Dirksen, Douglas A. Shoue, Douglas William Zochodne, Durga Krishnamurthy, Dustin Duncan, Dylan M. Glubb, Edoardo Luigi Maria Gelardi, Edward C. Hsiao, Edward G. Lynn, Elany Barbosa Silva, Elena Aguilera, Elena Lenci, Elena Theres Abraham, Eleonora Lama, Eleonora Mameli, Elisa Leung, Emily M. Christensen, Emily R. Mason, Enrico Petretto, Ephraim F. Trakhtenberg, Eric J. Rubin, Erick Strauss, Erik W. Thompson, Erika Cione, Erika Mathes Lisabeth, Erkang Fan, Erna Geessien Kroon, Eunji Jo, Eva M. García-Cuesta, Evgenia Glukhov, Evripidis Gavathiotis, Fang Yu, Fei Xiang, Fenfei Leng, Feng Wang, Filippo Ingoglia, Focco van den Akker, Francesco Borriello, Franco J. Vizeacoumar, Frank Luh, Frederick S. Buckner, Frederick S. Vizeacoumar, Fredj Ben Bdira, Fredrik Svensson, G. Marcela Rodriguez, Gabriella Bognár, Gaia Lembo, Gang Zhang, Garrett Dempsey, Gary Eitzen, Gaétan Mayer, Geoffrey L. Greene, George A. Garcia, Gergely L. Lukacs, Gergely Prikler, Gian Carlo G. Parico, Gianni Colotti, Gilles De Keulenaer, Gino Cortopassi, Giovanni Roti, Giulia Girolimetti, Giuseppe Fiermonte, Giuseppe Gasparre, Giuseppe Leuzzi, Gopal Dahal, Gracjan Michlewski, Graeme L. Conn, Grant David Stuchbury, Gregory R. Bowman, Grzegorz Maria Popowicz, Guido Veit, Guilherme Eduardo de Souza, Gustav Akk, Guy Caljon, Guzmán Alvarez, Gwennan Rucinski, Gyeongeun Lee, Gökhan Cildir, Hai Li, Hairol E. Breton, Hamed Jafar-Nejad, Han Zhou, Hannah P. Moore, Hannah Tilford, Haynes Yuan, Heesung Shim, Heike Wulff, Heinrich Hoppe, Helena Chaytow, Heng-Keat Tam, Holly Van Remmen, Hongyang Xu, Hosana Maria Debonsi, Howard B. Lieberman, Hoyoung Jung, Hua-Ying Fan, Hui Feng, Hui Zhou, Hyeong Jun Kim, Iain R. Greig, Ileana Caliandro, Ileana Corvo, Imanol Arozarena, Imran N. Mungrue, Ingrid M. Verhamme, Insaf Ahmed Qureshi, Irina Lotsaris, Isin Cakir, J. Jefferson P. Perry, Jacek Kwiatkowski, Jacob Boorman, Jacob Ferreira, Jacob Fries, Jadel Müller Kratz, Jaden Miner, Jair L. Siqueira-Neto, James G. Granneman, James Ng, James Shorter, Jan Hendrik Voss, Jan M. Gebauer, Janelle Chuah, Jarrod J. Mousa, Jason T. Maynes, Jay D. Evans, Jeffrey Dickhout, Jeffrey P. MacKeigan, Jennifer N. Jossart, Jia Zhou, Jiabei Lin, Jiake Xu, Jianghai Wang, Jiaqi Zhu, Jiayu Liao, Jingyi Xu, Jinshi Zhao, Jiusheng Lin, Jiyoun Lee, Joana Reis, Joerg Stetefeld, John B. Bruning, John Burt Bruning, John G. Coles, John J. Tanner, John M. Pascal, Jonathan So, Jordan L. Pederick, Jose A. Costoya, Joseph B. Rayman, Joseph J. Maciag, Joshua Alexander Nasburg, Joshua J. Gruber, Joshua M. Finkelstein, Joshua Watkins, José Miguel Rodríguez-Frade, Juan Antonio Sanchez Arias, Juan José Lasarte, Julen Oyarzabal, Julian Milosavljevic, Julie Cools, Julien Lescar, Julijus Bogomolovas, Jun Wang, Jung-Min Kee, Jung-Min Kee, Junzhuo Liao, Jyothi C. Sistla, Jônatas Santos Abrahão, Kamakshi Sishtla, Karol R. Francisco, Kasper B. Hansen, Kathleen A. Molyneaux, Kathryn A. Cunningham, Katie R. Martin, Kavita Gadar, Kayode K. Ojo, Keith S. Wong, Kelly L. Wentworth, Kent Lai, Kevin A. Lobb, Kevin M. Hopkins, Keykavous Parang, Khaled Machaca, Kien Pham, Kim Ghilarducci, Kim S. Sugamori, Kirk James McManus, Kirsikka Musta, Kiterie M. E. Faller, Kiyo Nagamori, Konrad J. Mostert, Konstantin V. Korotkov, Koting Liu, Kristiana S. Smith, Kristopher Sarosiek, Kyle H. Rohde, Kyu Kwang Kim, Kyung Hyeon Lee, Lajos Pusztai, Lari Lehtiö, Larisa M. Haupt, Leah E. Cowen, Lee J. Byrne, Leila Su, Leon Wert-Lamas, Leonor Puchades-Carrasco, Lifeng Chen, Linda H. Malkas, Ling Zhuo, Lizbeth Hedstrom, Lizbeth Hedstrom, Loren D. Walensky, Lorenzo Antonelli, Luisa Iommarini, Luke Whitesell, Lía M. Randall, M. Dahmani Fathallah, Maira Harume Nagai, Mairi Louise Kilkenny, Manu Ben-Johny, Marc P. Lussier, Marc P. Windisch, Marco Lolicato, Marco Lucio Lolli, Margot Vleminckx, Maria Cristina Caroleo, Maria J. Macias, Marilia Valli, Marim M. Barghash, Mario Mellado, Mark A. Tye, Mark A. Wilson, Mark Hannink, Mark R. Ashton, Mark Vincent C.dela Cerna, Marta Giorgis, Martin K. Safo, Martin St. Maurice, Mary Ann McDowell, Marzia Pasquali, Masfique Mehedi, Mateus Sá Magalhães Serafim, Matthew B. Soellner, Matthew G. Alteen, Matthew M. Champion, Maxim Skorodinsky, Megan L. O’Mara, Mel Bedi, Menico Rizzi, Michael Levin, Michael Mowat, Michael R. Jackson, Mikell Paige, Minnatallah Al-Yozbaki, Miriam A. Giardini, Mirko M. Maksimainen, Monica De Luise, Muhammad Saddam Hussain, Myron Christodoulides, Natalia Stec, Natalia Zelinskaya, Natascha Van Pelt, Nathan M. Merrill, Nathanael Singh, Neeltje A. Kootstra, Neeraj Singh, Neha S. Gandhi, Nei-Li Chan, Nguyen Mai Trinh, Nicholas O. Schneider, Nick Matovic, Nicola Horstmann, Nicola Longo, Nikhil Bharambe, Nirvan Rouzbeh, Niusha Mahmoodi, Njabulo Joyfull Gumede, Noelle C. Anastasio, Noureddine Ben Khalaf, Obdulia Rabal, Olga Kandror, Olivier Escaffre, Olli Silvennoinen, Ozlem Tastan Bishop, Pablo Iglesias, Pablo Sobrado, Patrick Chuong, Patrick O’Connell, Pau Martin-Malpartida, Paul Mellor, Paul V. Fish, Paulo Otávio Lourenço Moreira, Pei Zhou, Pengda Liu, Pengda Liu, Pengpeng Wu, Percy Agogo-Mawuli, Peter L. Jones, Peter Ngoi, Peter Toogood, Philbert Ip, Philipp von Hundelshausen, Pil H. Lee, Rachael B. Rowswell-Turner, Rafael Balaña-Fouce, Rafael Eduardo Oliveira Rocha, Rafael V. C. Guido, Rafaela Salgado Ferreira, Rajendra K. Agrawal, Rajesh K. Harijan, Rajesh Ramachandran, Rajkumar Verma, Rakesh K. Singh, Rakesh Kumar Tiwari, Ralph Mazitschek, Rama K. Koppisetti, Remus T. Dame, Renée N. Douville, Richard C. Austin, Richard E. Taylor, Richard G. Moore, Richard H. Ebright, Richard M. Angell, Riqiang Yan, Rishabh Kejriwal, Robert A. Batey, Robert Blelloch, Robert J. Vandenberg, Robert J. Hickey, Robert J. Kelm, Robert J. Lake, Robert K. Bradley, Robert M. Blumenthal, Roberto Solano, Robin Matthias Gierse, Ronald E. Viola, Ronan R. McCarthy, Rosa Maria Reguera, Ruben Vazquez Uribe, Rubens Lima do Monte-Neto, Ruggiero Gorgoglione, Ryan T. Cullinane, Sachin Katyal, Sakib Hossain, Sameer Phadke, Samuel A. Shelburne, Sandra E. Geden, Sandra Johannsen, Sarah Wazir, Scott Legare, Scott M. Landfear, Senthil K. Radhakrishnan, Serena Ammendola, Sergei Dzhumaev, Seung-Yong Seo, Shan Li, Shan Zhou, Shaoyou Chu, Shefali Chauhan, Shinsaku Maruta, Shireen R. Ashkar, Show-Ling Shyng, Silvestro G. Conticello, Silvia Buroni, Silvia Garavaglia, Simon J. White, Siran Zhu, Sofiya Tsimbalyuk, Somaia Haque Chadni, Soo Young Byun, Soonju Park, Sophia Q. Xu, Sourav Banerjee, Stefan Zahler, Stefano Espinoza, Stefano Gustincich, Stefano Sainas, Stephanie L. Celano, Stephen J. Capuzzi, Stephen N. Waggoner, Steve Poirier, Steven H. Olson, Steven O. Marx, Steven R. Van Doren, Suryakala Sarilla, Susann M. Brady-Kalnay, Sydney Dallman, Syeda Maryam Azeem, Tadahisa Teramoto, Tamar Mehlman, Tarryn Swart, Tatjana Abaffy, Tatos Akopian, Teemu Haikarainen, Teresa Lozano Moreda, Tetsuro Ikegami, Thaiz Rodrigues Teixeira, Thilina D. Jayasinghe, Thomas H. Gillingwater, Thomas Kampourakis, Timothy I. Richardson, Timothy J. Herdendorf, Timothy J. Kotzé, Timothy R. O’Meara, Timothy W. Corson, Tobias Hermle, Tomisin Happy Ogunwa, Tong Lan, Tong Su, Toshihiro Banjo, Tracy A. O’Mara, Tristan Chou, Tsui-Fen Chou, Ulrich Baumann, Umesh R. Desai, Vaibhav P. Pai, Van Chi Thai, Vasudha Tandon, Versha Banerji, Victoria L. Robinson, Vignesh Gunasekharan, Vigneshwaran Namasivayam, Vincent F. M. Segers, Vincent Maranda, Vincenza Dolce, Vinícius Gonçalves Maltarollo, Viola Camilla Scoffone, Virgil A. Woods, Virginia Paola Ronchi, Vuong Van Hung Le, W. Brent Clayton, W. Todd Lowther, Walid A. Houry, Wei Li, Weiping Tang, Wenjun Zhang, Wesley C. Van Voorhis, William A. Donaldson, William C. Hahn, William G. Kerr, William H. Gerwick, William J. Bradshaw, Wuen Ee Foong, Xavier Blanchet, Xiaoyang Wu, Xin Lu, Xin Qi, Xin Xu, Xinfang Yu, Xingping Qin, Xingyou Wang, Xinrui Yuan, Xu Zhang, Yan Jessie Zhang, Yanmei Hu, Yasser Ali Aldhamen, Yicheng Chen, Yihe Li, Ying Sun, Yini Zhu, Yogesh K. Gupta, Yolanda Pérez-Pertejo, Yong Li, Young Tang, Yuan He, Yuk-Ching Tse-Dinh, Yulia A. Sidorova, Yun Yen, Yunlong Li, Zachary J. Frangos, Zara Chung, Zhengchen Su, Zhenghe Wang, Zhiguo Zhang, Zhongle Liu, Zintis Inde, Zoraima Artía, Abraham Heifets, Ellie Giles Scientific Reports, 2024 As a result, the correct Affiliation for Andrew B. Herr, Benjamin Liou, David A. Hildeman, Joseph J. Maciag, Ying Sun, Durga Krishnamurthy, and Stephen N. Waggoner is:
Inhibiting HER3 Hyperphosphorylation in HER2-Overexpressing Breast Cancer through Multimodal Therapy with Branched Gold Nanoshells Eva Villar‐Alvarez, Irene Golán‐Cancela, Alberto Pardo, Brenda Velasco, Javier Fernández‐Vega, Adriana Cambón, Abeer Al‐Modlej, Antonio Topete, Silvia Barbosa, José A. Costoya, Pablo Taboada Small, 2023 Treatment failure in breast cancers overexpressing human epidermal growth factor receptor 2 (HER2) is associated mainly to the upregulation of human epidermal growth factor receptor 3 (HER3) oncoprotein linked to chemoresitence. Therefore, to increase patient survival, here a multimodal theranostic nanoplatform targeting both HER2 and HER3 is developed. This consists of doxorubicin‐loaded branched gold nanoshells functionalized with the near‐infrared (NIR) fluorescent dye indocyanine green, a small interfering RNA (siRNA) against HER3, and the HER2‐specific antibody Transtuzumab, able to provide a combined therapeutic outcome (chemo‐ and photothermal activities, RNA silencing, and immune response). In vitro assays in HER2+/HER3+ SKBR‐3 breast cancer cells have shown an effective silencing of HER3 by the released siRNA and an inhibition of HER2 oncoproteins provided by Trastuzumab, along with a decrease of the serine/threonine protein kinase Akt (p‐AKT) typically associated with cell survival and proliferation, which helps to overcome doxorubicin chemoresistance. Conversely, adding the NIR light therapy, an increment in p‐AKT concentration is observed, although HER2/HER3 inhibitions are maintained for 72 h. Finally, in vivo studies in a tumor‐bearing mice model display a significant progressively decrease of the tumor volume after nanoparticle administration and subsequent NIR light irradiation, confirming the potential efficacy of the hybrid nanocarrier.
A New Opportunity for “Old” Molecules: Targeting PARP1 Activity through a Non-Enzymatic Mechanism Pablo Iglesias, Marcos Seoane, Irene Golán-Cancela, Máximo Fraga, Victor M. Arce, Jose A. Costoya International Journal of Molecular Sciences, 2023 In recent years, new therapies have been developed based on molecules that target molecular mechanisms involved in both the initiation and maintenance of the oncogenic process. Among these molecules are the poly(ADP-ribose) polymerase 1 (PARP1) inhibitors. PARP1 has emerged as a target with great therapeutic potential for some tumor types, drawing attention to this enzyme and resulting in many small molecule inhibitors of its enzymatic activity. Therefore, many PARP inhibitors are currently in clinical trials for the treatment of homologous recombination (HR)-deficient tumors, BRCA-related cancers, taking advantage of synthetic lethality. In addition, several novel cellular functions unrelated to its role in DNA repair have been described, including post-translational modification of transcription factors, or acting through protein–protein interactions as a co-activator or co-repressor of transcription. Previously, we reported that this enzyme may play a key role as a transcriptional co-activator of an important component of cell cycle regulation, the transcription factor E2F1. Here, we show that PARP inhibitors, which interfere with its activity in cell cycle regulation, perform this without affecting its enzymatic function.
Editorial: Cellular stress in blood cancer: Pathophysiology and therapeutic opportunities José A. Costoya, Francesco Piazza Frontiers in Oncology, 2022 The cell can mitigate the possible damage induced by oxidative and other toxic agents preserving its survival. The activation of different pathways, with the purpose of maintaining key cellular functions, will assure the cellular homeostasis despite a hostile environment. Among the intracellular mechanisms that are activated in response to different stresses are DNA damage response (DDR), mitochondrial stress signaling and autophagy, and in many cases these mechanisms will regulate cell cycle, cell death response and senescence that elicit a systemic response (1). These responses produce modifications of stressed cells associated to secretion of soluble factors or microvesicles, that in some cases are linked to disease, such as cancer (1). In fact, cancer cells exploit these responses to adapt and survive to many types of dangerous noxae related to the oncogenic process, such as hypoxia, nutrient deprivation, and the associated metabolic and oxidative stress. These mechanisms are key for cell survival allowing cancer progression and resistance to therapy, as well as evasion of immune surveillance. Thus, the understanding of the molecular mechanisms of cancer response to stress can provide fundamental insights to recognize new potential therapeutic targets and improve clinical management (2).
Development of a biosensor based on a new marine luciferase fused to an affibody to assess Her2 expression in living cells Laura Rodríguez de la Fuente, Irene Golán Cancela, Ánxela M. Estévez-Salguero, Pablo Iglesias, José A. Costoya Analytica Chimica Acta, 2022 The development of new diagnostic tools in tumor pathology allows the optimization of individualized therapies in cancer patients. The functional optical image provides a unique opportunity to identify the pathophysiological characteristics of each tumor in a non-invasive way. Although fluorescent recombinant affibodies and nanobodies, capable of detecting certain membrane proteins present in tumor cells, has been described, the use of bioluminescent molecules is gaining a great impact in this field due to its high sensitivity. In this work, we characterize a new luciferase from the Metridia lucens copepod (MlLuc) and develop a novel bioluminescent recombinant affibody (MlLuc-aff) capable of recognizing the HER2 receptors that are overexpressed in breast cancer tumors. For this purpose, the thermostability and pH sensitivity of MlLuc1.1 were determined, showing no significant changes in the activity among temperatures between 4 and 70 °C, and with a maximum of brightness at pH 8.0. Furthermore, MlLuc-aff was able to accurately detect HER2 receptors expressed in the SK-BR-3 cells. Future applications of this new tracer can contribute to the early diagnosis of breast cancer patients and the assessment of the efficacy of the treatment.
Development of a nanocapsule-loaded hydrogel for drug delivery for intraperitoneal administration Bhanu Teja Surikutchi, Rebeca Obenza-Otero, Emanuele Russo, Mischa Zelzer, Irene Golán Cancela, José A. Costoya, José Crecente Campo, Maria José Alonso, Maria Marlow International Journal of Pharmaceutics, 2022 Intraperitoneal (IP) drug delivery of chemotherapeutic agents, administered through hyperthermal intraperitoneal chemotherapy (HIPEC) and pressurized intraperitoneal aerosolized chemotherapy (PIPAC), is effective for the treatment of peritoneal malignancies. However, these therapeutic interventions are cumbersome in terms of surgical practice and are often associated with the formation of peritoneal adhesions, due to the catheters inserted into the peritoneal cavity during these procedures. Hence, there is a need for the development of drug delivery systems that can be administered into the peritoneal cavity. In this study, we have developed a nanocapsule (NCs)-loaded hydrogel for drug delivery in the peritoneal cavity. The hydrogel has been developed using poly(ethylene glycol) (PEG) and thiol-maleimide chemistry. NCs-loaded hydrogels were characterized by rheology and their resistance to dilution and drug release were determined in vitro. Using IVIS® to measure individual organ and recovered gel fluorescence intensity, an in vivo imaging study was performed and demonstrated that NCs incorporated in the PEG gel were retained in the IP cavity for 24 h after IP administration. NCs-loaded PEG gels could find potential applications as biodegradable, drug delivery systems that could be implanted in the IP cavity, for example at a the tumour resection site to prevent recurrence of microscopic tumours.
Bioluminescence resonant energy transfer as a basis for a novel hypoxia biosensor for In Vivo functional imaging Bioluminescence Characteristics Adaptations and Biotechnology, 2011
Role of growth hormone receptor in HL-60 cell survival José A. Costoya, Ramón Rı́os, Mónica Garcı́a-Barros, Rosalı́a Gallego, Tomás Garcı́a-Caballero, Rosa Señarı́s, Vı́ctor M. Arce, Jesús Devesa Molecular Cell Biology Research Communications, 2000
Solubilization and Selective Integrin-targeted Delivery of Polycyclic Aromatic Hydrocarbons with Amphiphilic α-Helices A Sarmiento, R Lopez-Blanco, L González-Rendo, N Barreiro-Piñeiro, ... 2026
Neural stem cells and glioblastoma stem cells: redefining concepts VM Arce, L González-Rendo, L Porres-Ventín, V González-Álvarez, ... Critical Reviews in Oncology/Hematology, 105187 , 2026 2026
Functional and evolutionary diversification of luciferase genes in Metridia lucens Boeck 1865 LB Gabín-García, C Bartolomé, P Iglesias, ÁM Estévez-Salguero, ... Scientific Reports , 2026 2026
Validation of the antitumor compound 3797 in human and murine glioblastoma models L Gonzalez-Rendo, D Moreiras-Alvarez, M Baleiron-Rodriguez, ... Cancer Research 85 (8_Supplement_1), 4299-4299 , 2025 2025
AB067. New drug development for the use of PARP1-E2F1 transcriptional inhibitors in the treatment of glioblastoma VM Arce, P Iglesias, D Moreira, L Porres-Ventin, L Gonzalez-Rendo, ... Chinese Clinical Oncology 13 (Suppl 1), AB067 , 2024 2024
AI is a viable alternative to high throughput screening: a 318-target study Scientific reports 14 (1), 7526 , 2024 2024 Citations: 102
Shedding light on PARP1-E2F1 interaction by Cryo-EM P Iglesias, E Casajus-Pelegay, M Moreno-Morcillo, L Gonzalez-Rendo, ... Cancer Research 84 (6_Supplement), 5785-5785 , 2024 2024
New drug development opportunities for PARP1 P Iglesias, D Moreiras, L Porres-Ventin, L Gonzalez-Rendo, JA Zumalave, ... Cancer Research 84 (6_Supplement), 4483-4483 , 2024 2024
PARP1: Opportunities of Developing New Drugs for an Old Target VM Arce, P Iglesias, JA Costoya Springer, Cham , 2024 2024
Inhibiting HER3 Hyperphosphorylation in HER2‐Overexpressing Breast Cancer through Multimodal Therapy with Branched Gold Nanoshells E Villar‐Alvarez, I Golán‐Cancela, A Pardo, B Velasco, ... Small 19 (50), 2303934 , 2023 2023 Citations: 11
P10. 36. B ROLE OF AMPK IN GLIOBLASTOMA BIOENERGETICS S Caamaño-Teixeira, C Iglesias, V Gonzalez-Alvarez, V Arce, J Costoya, ... Neuro-Oncology 25 (Suppl 2), ii71 , 2023 2023
A new opportunity for “old” molecules: targeting PARP1 activity through a non-enzymatic mechanism P Iglesias, M Seoane, I Golan-Cancela, M Fraga, VM Arce, JA Costoya International Journal of Molecular Sciences 24 (10), 8849 , 2023 2023 Citations: 6
PARP1: New Drug Development Opportunities for an Old Target P Iglesias, M Seoane, I Golán-Cancela, M Fraga, VM Arce, JA Costoya Preprints , 2023 2023 Citations: 1
Cancer cells escape the immune system by increasing stemness through epigenetic reprogramming JA Costoya, VM Arce Cellular & Molecular Immunology 20 (1), 6-7 , 2023 2023 Citations: 21
Cellular stress in blood cancer: Pathophysiology and therapeutic opportunities JA Costoya, F Piazza Frontiers in Oncology 12, 1026315 , 2022 2022 Citations: 3
Development of a biosensor based on a new marine luciferase fused to an affibody to assess Her2 expression in living cells LR de la Fuente, IG Cancela, AM Estevez-Salguero, P Iglesias, ... Analytica Chimica Acta 1221, 340084 , 2022 2022 Citations: 10
Development of a nanocapsule-loaded hydrogel for drug delivery for intraperitoneal administration B Teja Surikutchi, R Obenza-Otero, E Russo, M Zelze, I Golán Cancela, ... Int J Pharm 622 (121828) , 2022 2022 Citations: 27
New insights into the relevance of mobile DNA to cancer resistance in blind mole rats: new lessons for cancer therapy VM Arce, JA Costoya Cellular & Molecular Immunology 19 (4), 458-459 , 2022 2022
From neural stem cells to glioblastoma: A natural history of GBM recapitulated in vitro C Almenglo, P Caamano, M Fraga, J Devesa, JA Costoya, VM Arce Journal of Cellular Physiology 236 (11), 7390-7404 , 2021 2021 Citations: 9
SARS-CoV-2 infection in K18-ACE2 transgenic mice replicates human pulmonary disease in COVID-19 VM Arce, JA Costoya Cellular & Molecular Immunology 18 (3), 513-514 , 2021 2021 Citations: 56
MOST CITED SCHOLAR PUBLICATIONS
Essential role of Plzf in maintenance of spermatogonial stem cells JA Costoya, RM Hobbs, M Barna, G Cattoretti, K Manova, M Sukhwani, ... Nature genetics 36 (6), 653-659 , 2004 2004 Citations: 1141
Fluorescent drug-loaded, polymeric-based, branched gold nanoshells for localized multimodal therapy and imaging of tumoral cells A Topete, M Alatorre-Meda, P Iglesias, EM Villar-Alvarez, S Barbosa, ... ACS nano 8 (3), 2725-2738 , 2014 2014 Citations: 212
Plzf mediates transcriptional repression of HoxD gene expression through chromatin remodeling M Barna, T Merghoub, JA Costoya, D Ruggero, M Branford, A Bergia, ... Developmental cell 3 (4), 499-510 , 2002 2002 Citations: 209
AMPK activation by oncogenesis is required to maintain cancer cell proliferation in astrocytic tumors M Ríos, M Foretz, B Viollet, A Prieto, M Fraga, JA Costoya, R Señarís Cancer research 73 (8), 2628-2638 , 2013 2013 Citations: 154
Activation of growth hormone receptor delivers an antiapoptotic signal: evidence for a role of Akt in this pathway JA Costoya, J Finidori, S Moutoussamy, R Señaris, J Devesa, VM Arce Endocrinology 140 (12), 5937-5943 , 1999 1999 Citations: 138
Functional analysis of the role of POK transcriptional repressors JA Costoya Briefings in Functional Genomics and Proteomics 6 (1), 8-18 , 2007 2007 Citations: 133
Activation of human somatostatin receptor 2 promotes apoptosis through a mechanism that is independent from induction of p53 R Teijeiro, R Ríos, J Costoya, R Castro, J Bello, J Devesa, V Arce Cellular Physiology and Biochemistry 12 (1), 31-38 , 2002 2002 Citations: 123
AI is a viable alternative to high throughput screening: a 318-target study Scientific reports 14 (1), 7526 , 2024 2024 Citations: 102
NK cell-based glioblastoma immunotherapy I Golan, L Rodriguez de la Fuente, JA Costoya Cancers 10 (12), 522 , 2018 2018 Citations: 82
Disruption of PLZP in mice leads to increased T-lymphocyte proliferation, cytokine production, and altered hematopoietic stem cell homeostasis F Piazza, JA Costoya, T Merghoub, RM Hobbs, PP Pandolfi Molecular and cellular biology 24 (23), 10456-10469 , 2004 2004 Citations: 64
The role of promyelocytic leukemia zinc finger and promyelocytic leukemia in leukemogenesis and development JA Costoya, PP Pandolfi Current opinion in hematology 8 (4), 212-217 , 2001 2001 Citations: 60
Expression of growth hormone receptor in the human brain JR Castro, JA Costoya, R Gallego, A Prieto, VM Arce, R Señarı́s Neuroscience letters 281 (2-3), 147-150 , 2000 2000 Citations: 59
SARS-CoV-2 infection in K18-ACE2 transgenic mice replicates human pulmonary disease in COVID-19 VM Arce, JA Costoya Cellular & Molecular Immunology 18 (3), 513-514 , 2021 2021 Citations: 56
Retinoblastoma loss modulates DNA damage response favoring tumor progression M Seoane, P Iglesias, T Gonzalez, F Dominguez, M Fraga, C Aliste, ... PLoS One 3 (11), e3632 , 2008 2008 Citations: 46
A novel BRET-based genetically encoded biosensor for functional imaging of hypoxia P Iglesias, JA Costoya Biosensors and Bioelectronics 24 (10), 3126-3130 , 2009 2009 Citations: 45
The mitochondrial genome is a “genetic sanctuary” during the oncogenic process M Seoane, A Mosquera-Miguel, T Gonzalez, M Fraga, A Salas, ... PloS one 6 (8), e23327 , 2011 2011 Citations: 33
Lipoprotein internalisation induced by oncogenic AMPK activation is essential to maintain glioblastoma cell growth M Rios, M Foretz, B Viollet, A Prieto, M Fraga, T García-Caballero, ... European Journal of Cancer 50 (18), 3187-3197 , 2014 2014 Citations: 30
Uncoupling Oncogene-Induced Senescence (OIS) and DNA Damage Response (DDR) triggered by DNA hyper-replication: lessons from primary mouse embryo astrocytes (MEA) M Seoane, JA Costoya, VM Arce Scientific reports 7 (1), 12991 , 2017 2017 Citations: 28
NIR-light active hybrid nanoparticles for combined imaging and bimodal therapy of cancerous cells A Topete, D Melgar, M Alatorre-Meda, P Iglesias, B Argibay, S Vidawati, ... Journal of Materials Chemistry B 2 (40), 6967-6977 , 2014 2014 Citations: 28
