C. Janani
@andavancollege.ac.in
Assistant Professor, Biotechnology
Srimad Andavan Arts and Science College (Autonomous), Trichy-05
Gender : Female
Marital status : Married
Date of Birth : 23.08.1987
Languages Known : English, Tamil
Nationality : Indian
Religion : Hindu, Kantharvakottai Kallar
EDUCATION
M.Sc., Ph.D.
RESEARCH INTERESTS
Plant Biotechnology, Plant Genetic Engineering, Stress Biology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
In recent years, plants have been used as reducing agents for the synthesis of nanoparticles in a short time, and it consists of low cost, and there is no harmful effect on the environment; In this study, silver nanoparticles are synthesized from AgNO3 using leaf extract of Solena amplexicaulis (Lam.) Gandhi. The leaf extract is mixed with AgNO3 solution, and color change occurs from whitish-yellow to dark brown at room temperature within a few minutes. After incubation, the suspension was centrifuged, and particles were separated and dried. The green synthesized AgNPs were characterized by UV-visible spectroscopy in a maximum wavelength of 393 nm, FTIR, SEM, EDAX, DLS, XRD, and ZETA POTENTIAL; Photocatalytic degradation of Victoria blue or basic blue dye was investigated by green synthesized Ag nanoparticles with solar irradiation technique by biometrically at different time intervals. The UV-visible characteristic peak absorption of Victoria blue solution was 250 nm. The photocatalytic activity at different time intervals [control, treated controls, 30 min, (1, 2, 3, 5, 8, 12, 24, 48, 60, 72) hrs] was collected and decreased the peak intensity and was measured using UV-visible spectroscopy and Fourier Transform Infrared Spectroscopy. Green synthesized Ag nanoparticles effectively degraded the Victoria blue dye solution in total exposure time.
S. M. Fathima Khyrun, Z. Mohamed Riyas, Vaishnavi Raja, Sulthana Sabura Sarbudeen, Vinothini Natesan, K. Velsankar, S. Sudhahar, M.Ramesh Prabhu, Mydhili Govindarasu, Muthu Thiruvengadam,et al.
Elsevier BV
K. Prabhu, P. Sudharsan, P. Ganesh Kumar, B. Chitra, and C. Janani
Elsevier BV
C. Janani, B. Sundararajan, and B.D. Ranjitha Kumari
Elsevier BV
Abstract The PPARγ gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) nuclear receptors subfamily which forms heterodimers with retinoid X receptors (RXRs) and they regulate transcription of various genes. In the present study, the binary vector of pBI121 was constructed with RnPPARγ gene (1428 bp) of Rattus norvegicus under CaMV35s promoter and also kanamycin resistance gene available for selecting the transforms. Transformation of soybean was conducted for half seed explants of JS335 variety using strain EHA105 harboring pBI121- RnPPARγ. Transformation conditions were standardized for soybean, and the results demonstrate that L-Cysteine (1 mM) has improved the regeneration of transformants. The combination of TDZ (10 μM) and L-Cysteine (1 mM) induced a maximum of 80% shooting, whereas IBA (15 μM) and L-Cysteine (1 mM) combination induced a maximum of 83% rooting frequency. PCR and Southern Blotting techniques confirmed the transgene integration. These study findings suggest that L-Cysteine (1 mM) involvement increased the transfer of T-DNA during transformation and the gene delivered under the suitable condition for regeneration and also enhanced the transformation efficiency in soybean through direct (1.6%), indirect (1.2%) and in planta (2.0%) method of transformation.
C. Janani and B.D. Ranjitha Kumari
Elsevier BV
Peroxisome proliferator-activated receptor gamma (PPARγ) has been the focus of intense research because ligands for this receptor have emerged as potent insulin sensitizers used in the treatment of type 2 diabetes. There have been described three PPAR isotypes α, δ and γ which have an integrated role in controlling the expression of genes playing key roles in the storage and mobilization of lipids, in glucose metabolism, in morphogenesis and inflammatory response. Recent advances include the discovery of novel genes that are regulated by PPARγ, which helps to explain how activation of this adipocyte predominant transcription factor regulates glucose and lipid homeostasis. Increased levels of circulating free fatty acids and lipid accumulation in non-adipose tissue have been implicated in the development of insulin resistance. This situation is improved by PPARγ ligands, which promotes fatty acid storage in fat deposits and regulates the expression of adipocyte-secreted hormones that impacts on glucose homeostasis. So the net result of the pleiotropic effects of PPARγ ligands is improvement of insulin sensitivity. This review highlights the roles that PPAR gamma play in the regulation of gene expression of multiple diseases including obesity, diabetes and cancer and highlights the gene isolation transformation role. Further studies are needed for the transformation of PPAR gamma gene in plants and evaluate in animals for the treatment of type 2 diabetes.
RECENT SCHOLAR PUBLICATIONS
MOST CITED SCHOLAR PUBLICATIONS
Publications
• Janani C, Girija S. and Ranjitha Kumari B. D. In vitro Culture and Agrobacterium Mediated Transformation in High Altitude Tomato (Lycopersicon esculentum Mill.) Cultivar Shalimar. International Journal of Pharmacy Teaching & Practices, 2013, 4, 1, 483-488. (Citations 6).
• Janani C and Ranjitha Kumari B.D. In vitro plant regeneration from cotyledonary node and half seed explants of Glycine max L. (JS335). Annals of Biological Research. 2013, 4 (11). 60-66. (Citations 5).
• Janani C and Ranjitha Kumari B.D. PPAR Gamma Gene - A Review. Diabetes & Metabolic Syndrome: Clinical Research & Reviews. 2015. 46–50. (Citations 588).
• Janani C and Ranjitha Kumari B.D. In vitro Regeneration and Biohardening of Soybean (JS335) Variety. International Journal of Science and Research. 2016. 5:5, 1653-1656.
• Janani C., Ranjitha Kumari B.D. Antidiabetic Activity of Methanolic Leaves Extracts of Transformed Soybean in 3T3-L1 Pre-Adipocytes Cell Line. International Journal of Pharmaceutical Sciences and Research. 2017. 8(11): 4732-4744. (Citation 1)
• Janani C., Sundararajan B., Ranjitha Kumari B.D. Construction and transformation of peroxisome proliferator activated receptor gamma (RnPPARγ) gene using Agrobacterium tumefaciens into Glycine max L. Merr. Gene Reports. 2019. 100427.
• Janani C., and Ranjitha Kumari B.D. Enumeration of Sodium Chloride Illness on Regeneration by Ethyl Methane Sulphonate (EMS) T