From a By-Product to an Active Raw Material: the Use of Brewers’ Spent Grain Extract in Cosmetic Masks Ana Beatriz Klosowski, Aline Carvalho Lopes, Mateus Gonçalves Prado Balado, Patrícia Mazureki Campos, Juliana Bonametti Olivato Brazilian Archives of Biology and Technology, 2025 Brewers’ spent grain (BSG) is the primary by-product of the brewing industry, being a source of phenolic compounds associated with antioxidant activity. In this study, starch/polyvinyl alcohol (PVA) films with BSG extract were developed as an alternative anti-aging and sustainable cosmetic mask. The influence of BSG extract (concentrations of 5% and 10%) on the structural, thermal, mechanical, and barrier properties of the starch/PVA films was evaluated and compared with the Control film (without BSG extract). The in vitro release of phenolic compounds from BSG extract incorporated in the films in water and skin simulant medium (pH 5.5) was also evaluated. By increasing the BSG extract concentration to 10%, the film matrix became more cohesive and homogeneous, as demonstrated by the FEG-SEM images, resulting in greater tensile strength when compared to the Control and 5% BSG extract films. The films with 10% BSG extract were more rigid (lower elastic modulus) and brittle (lower elongation at break). The yellow extract coloration increased the opacity of the film with 10% BSG extract and a reduction in water vapor permeability due to the lipophilic character of the extract. After 4 hours, all films evaluated showed a complete release of phenolic compounds from BSG extract, which indicates their active role as anti-aging cosmetics. Based on the results, films with 5% BSG extract present interesting characteristics to be a natural and eco-friendly alternative to facial beauty masks.
How Are the Properties of Starch-Based Composites Influenced by the Chemical Treatment of BSG? Georgia W. Ramos, Ana B. Klosowski, Aline C. Lopes, Giovani A. Carvalho, Juliana B. Olivato Macromolecular Symposia, 2024 Lignocellulosic fibers from Brewer's spent grain (BSG) represent an ecologically and economically favorable alternative that can act as reinforcing agents for starch‐based polymer composites. Biodegradable composites are produced using cassava starch and BSG of the Pilsen (P) and Weiss (W) types, which are subjected to mercerization (BSM), bleaching (BSP) and a combination of those treatments (BSB). By including treated BSGs, homogeneous matrices with starch are produced, without the presence of pores and/or cracks, as evidenced by the scanning electron microscopy (SEM) images. The tensile strength of the composites is improved by up to 80% and Young's modulus by up to 50% with the addition of BSB(P) and BSB(W) fibers, indicating the role of the materials as reinforcement agents. Bleaching and mercerization treatments are efficient in the exposition of hydroxyl groups of cellulose, resulting in higher hydrophilicity and water vapor permeability (WVP) of the composites containing the BSP and BSM fibers. Biodegradable composites incorporating BSG from the brewing industry have promising properties and can be a viable alternative to non‐biodegradable polymeric packaging.
Active Biodegradable Starch/PBAT-poly(butylene adipate-co-terephthalate) Film with Eucalyptus citriodora Essential Oil Incorporation Juliano Zanela, Marianne Ayumi Shirai, Juliana Bonametti Olivato, Maira Casagrande, Cristian Medrado Canonico, et al. Foods, 2024 Plastic pollution and the reduction in synthetic food additives are demands that emerge from consumers, leading to the development of biodegradable plastic materials. The use of essential oils—EOs—has been researched because it is a natural product with antioxidant properties. Due to its nature, EO is composed of volatile compounds that can be lost during extrusion. The aim of this work was to produce active biodegradable starch/PBAT films with the incorporation of neat Eucalyptus citriodora EO (0.5, 1.0, and 1.5%) or EO microencapsulated by spray drying (2.5, 5.0, and 7.5%), aiming at the protection of the EO. The produced films showed adequate mechanical properties (tensile strength ranged from 5.72 to 7.54 MPa and the elongation at break ranged from 319 to 563%). Testing in food simulants showed that the films retained antioxidant activity, being more suitable for use in fatty or non-acid foods, with the microencapsulation process offering protection to the EO during the process.
Brewers' spent grain extract as antioxidants in starch-based active biopolymers Flavia Rafaela Ludka, Ana Beatriz Klosowski, Giovani Almeida Camargo, Aline Silva Justo, Evelyn Assis Andrade, et al. International Journal of Food Science and Technology, 2024 SummaryBrewer's spent grain (BSG) is a by‐product of the brewery and represents a sustainable alternative for application in starch‐based active materials. This study aimed to develop starch/poly(vinyl alcohol) films containing BSG extract, with focus on the influence of BSG on the mechanical, structural, and barrier properties of the films. The antioxidant potentials of the extract and the films were proved by ABTS●+ (IC50 = 2.0 ± 0.6 μg mL−1) and DPPH● (IC50 = 196.05 ± 018.66 μg mL−1) assays. The addition of BSG extract did not have a significant impact on the mechanical performance and weight loss in water of the materials. The incorporation of the extract resulted in a rough surface, without pores and/or cracks. Controlled release of phenolic compounds was observed for BSG3 (3 g.100 g−1 of BSG extract) (163.3 ± 20.9 mgGAE.mL−1) and BSG5 (5 g.100 g−1 of BSG extract) (165.7 ± 23.6 mgGAE.mL−1) over a 4 h period. Therefore, this study shows the potential of BSG as an organic source of antioxidants for active biopolymers.
Study of the Influence of the Process Parameters in the Characteristics of Silver-loaded Zeolite Complexes Aline C. Lopes, Ana B. Klosowski, Priscileila C. Ferrari, Luis A. Esmerino, Juliana B. Olivato Chemistryselect, 2023 Zeolite has a great capacity of adsorption and ion exchange. Silver ions are impregnated into the zeolite matrix by ion exchange, which results in the formation of an antimicrobial complex. By using a factorial design, this study explores the influence of pH, temperature, and silver nitrate concentration on the characteristics of ZeAg complexes. The ZeAg8 complex, produced with the factors of [AgNO 3 ] of 4000 mg/L, pH 5 and T 45 °C showed promising results, showing antimicrobial activity, and maintained an original crystalline structure of the zeolite after the experimental conditions. Experimental design analysis showed that lower pH and temperature favored larger particle diameter and induce disruption of the crystalline structure of the zeolite. Silver ions impregnation into zeolite under specific conditions create a potential antimicrobial complex for wound dressings, promoting tissue regeneration and healing while preventing potential complications associated with silver treatment.
Production and Characterization of Starch/PVA Blends with Bioactive Compounds from Malt Bagasse Lais C. M. Almeida, Francieli K. Carvalho, Juliana B. Olivato Macromolecular Symposia, 2022 Starch is an abundant and renewable material for producing biodegradable films and, to improve their mechanical performance is usually blended with polymers of better performance, as poly(vinyl alcohol) (PVA). Malt bagasse or brewer spent grain is a by‐product of brewing, mostly used as animal feed, and has bioactive compounds with proven antioxidant properties. On this basis, this work aims to produce starch‐PVA blends incorporated with malt bagasse extracts of two different types of beer, Pilsen, and Weiss. The inclusion of the Weiss (WMB) and Pilsen (PMB) malt bagasse extracts has no significant influence on the water solubility of the films. FEG‐SEM images present no tendency to phase separation at the sample surfaces, with a higher roughness of samples containing PMB extracts and improved homogeneity with the presence of WMB extracts. The inclusion of the bioactive extracts, regardless of concentration, has preserved the resistance and flexibility of the blends. Thermogravimetric analysis shows that all the extracts have a positive influence on the thermal stability of the materials. The starch/PVA biodegradable blends provide an alternative for producing active films suitable for cosmetic uses as anti‐aging masks.
Starch Hydrogel Films with Dual Cross-Linking: Structural and Functional Characterization AC Lopes, AB Klosowski, JB Olivato ACS omega 11 (7), 11808-11817 , 2026 2026
Packaging: Technological Alternatives in the Food Industry RL Coutrim, RA Ayub, RA Prestes, JB Olivato, M Zucoloto Nanobiotechnology for Postharvest Management, 331-358 , 2025 2025
PLA/PHBV/SEP/ZnO Hybrid Composites for Antimicrobial Multifunctional Packaging AV Duarte, HL Lopes, BCS Luca, MGP Balado, AB Klosowski, JB Olivato, ... Materials Research 28 (Suppl 1), e20250046 , 2025 2025 Citations: 2
From a By-Product to an Active Raw Material: the Use of Brewers’ Spent Grain Extract in Cosmetic Masks AB Klosowski, AC Lopes, MGP Balado, PM Campos, JB Olivato Brazilian Archives of Biology and Technology 68, e25241019 , 2025 2025 Citations: 1
Starch nanoparticles with brewers’ spent grain extract as stabilizers for Pickering emulsions: a biodegradable and sustainable alternative for natural cosmetics AB Klosowski, BVF Riccio, K de Castro Pereira, MGP Balado, AC Lopes, ... Observatório de la economía latinoamericana 23 (4), e9711-e9711 , 2025 2025 Citations: 3
Polysaccharide Films as Platforms for Wound Dressing Treatment FK de Carvalho, PM Campos, JB Olivato, PC Ferrari, MK Mishra CRC Press , 2025 2025 Citations: 1
Production and Characterization of Biodegradable Wound Dressings Containing Silver-Loaded Zeolite Complexes AC Lopes, AB Klosowski, JB Olivato ACS omega 10 (12), 12185-12191 , 2025 2025 Citations: 2
How are the properties of starch‐based composites influenced by the chemical treatment of BSG? GW Ramos, AB Klosowski, AC Lopes, GA Carvalho, JB Olivato Macromolecular Symposia 413 (6), 2400081 , 2024 2024 Citations: 2
Biodegradable active packaging based on an antioxidant extract from brewer’s spent grains: Development and potential of application FJA Vieira, FR Ludka, KM Diniz, AB Klosowski, JB Olivato ACS Sustainable Resource Management 1 (11), 2413-2419 , 2024 2024 Citations: 12
Assessing the degradability of biodegradable-packaging base material through the insertion of organic solid waste deriving from composting process MV da Luz, CCB da Silva Cruz, JB Olivato, TB Veiga Revista Brasileira de Ciência, Tecnologia e Inovação 9 (1), 1-14 , 2024 2024
Active Biodegradable Starch/PBAT-poly(butylene adipate-co-terephthalate) Film with Eucalyptus citriodora Essential Oil Incorporation J Zanela, MA Shirai, JB Olivato, M Casagrande, CM Canonico, ... Foods 13 (13), 2104 , 2024 2024 Citations: 7
Agriculture 4.0 for postharvest of fruit: a review. GA Camargo, A BÜHLMANN, F BÜCHELE, C KESKE, JB OLIVATO, ... 2024
Starch: A natural, safe, and multifunctional ingredient for cosmetic formulations JB Olivato Starch Industries: Processes and Innovative Products in Food and Non-Food … , 2024 2024 Citations: 9
Brewers' spent grain extract as antioxidants in starch-based active biopolymers FR Ludka, AB Klosowski, GA Camargo, AS Justo, EA Andrade, ... International Journal of Food Science and Technology 59 (1), 142-150 , 2024 2024 Citations: 20
Compatibilizantes na extrusão reativa de blendas amido/poli (butileno adipato co-tereftalato) para produção de filmes biodegradáveis JB Olivato 2024 Citations: 1
Study of the Influence of the Process Parameters in the Characteristics of Silver‐loaded Zeolite Complexes AC Lopes, AB Klosowski, PC Ferrari, LA Esmerino, JB Olivato ChemistrySelect 8 (45), e202303960 , 2023 2023 Citations: 2
Production and characterization of starch/PVA blends with bioactive compounds from malt bagasse LCM Almeida, FK Carvalho, JB Olivato Macromolecular Symposia 406 (1), 2200013 , 2022 2022 Citations: 5
Application and characterisation of industrial brewing by-products in biodegradable starch-based expanded composites AC Lopes, AB Klosowski, BM Carvalho, JB Olivato International Journal of Food Science and Technology 57 (8), 5523-5531 , 2022 2022 Citations: 10
Halloysite/cellulose derivatives-based bionanocomposites for controlled naproxen delivery JB de Macedo, JB Olivato, J Marini, J Aenishanslin, SA Pianaro, ... J Appl Polym Sci , 2022 2022
Citric acid and maleic anhydride as compatibilizers in starch/poly (butylene adipate-co-terephthalate) blends by one-step reactive extrusion JB Olivato, MVE Grossmann, F Yamashita, D Eiras, LA Pessan Carbohydrate Polymers 87 (4), 2614-2618 , 2012 2012 Citations: 198
Effect of organic acids as additives on the performance of thermoplastic starch/polyester blown films JB Olivato, MVE Grossmann, AP Bilck, F Yamashita Carbohydrate Polymers 90 (1), 159-164 , 2012 2012 Citations: 192
Citric acid as crosslinking agent in starch/xanthan gum hydrogels produced by extrusion and thermopressing BM Simões, C Cagnin, F Yamashita, JB Olivato, PS Garcia, ... Lwt 125, 108950 , 2020 2020 Citations: 126
Biodegradable trays of thermoplastic starch/poly (lactic acid) coated with beeswax MO Reis, JB Olivato, AP Bilck, J Zanela, MVE Grossmann, F Yamashita Industrial Crops and Products 112, 481-487 , 2018 2018 Citations: 122
Elaboration, morphology and properties of starch/polyester nano-biocomposites based on sepiolite clay JB Olivato, J Marini, E Pollet, F Yamashita, MVE Grossmann, L Avérous Carbohydrate Polymers 118, 250-256 , 2015 2015 Citations: 112
Thermoplastic starch/polyester films: Effects of extrusion process and poly (lactic acid) addition MA Shirai, JB Olivato, PS Garcia, CMO Müller, MVE Grossmann, ... Materials Science and Engineering: C 33 (7), 4112-4117 , 2013 2013 Citations: 92
Sepiolite as a promising nanoclay for nano-biocomposites based on starch and biodegradable polyester JB Olivato, J Marini, F Yamashita, E Pollet, MVE Grossmann, L Avérous Materials Science and Engineering: C 70, 296-302 , 2017 2017 Citations: 87
Mixture design applied for the study of the tartaric acid effect on starch/polyester films JB Olivato, MM Nobrega, CMO Müller, MA Shirai, F Yamashita, ... Carbohydrate Polymers 92 (2), 1705-1710 , 2013 2013 Citations: 84
Physical and structural characterisation of starch/polyester blends with tartaric acid JB Olivato, CMO Müller, GM Carvalho, F Yamashita, MVE Grossmann Materials Science and Engineering: C 39, 35-39 , 2014 2014 Citations: 81
Biodegradable starch-based films containing saturated fatty acids: thermal, infrared and Raman spectroscopic characterization MM Nobrega, JB Olivato, CMO Müller, F Yamashita Polímeros 22 (5), 475-480 , 2012 2012 Citations: 81
Compatibilisation of starch/poly (butylene adipate co-terephthalate) blends in blown films JB Olivato, MVE Grossmann, F Yamashita, MM Nobrega, MRS Scapin, ... International Journal of Food Science and Technology 46 (9), 1934-1939 , 2011 2011 Citations: 57
Using glycerol produced from biodiesel as a plasticiser in extruded biodegradable films AP Bilck, CMO Müller, JB Olivato, S Mali, MVE Grossmann, F Yamashita Polímeros 25, 331-335 , 2015 2015 Citations: 46
Biodegradable bags for the production of plant seedlings AP Bilck, JB Olivato, F Yamashita, JRP Souza Polímeros 24, 547-553 , 2014 2014 Citations: 45
Microcrystalline Cellulose as Reinforcement in Thermoplastic Starch/Poly(butylene adipate- co -terephthalate) Films MO Reis, J Zanela, J Olivato, PS Garcia, F Yamashita, MVE Grossmann Journal of Polymers and the Environment 22 (4), 545-552 , 2014 2014 Citations: 43
Mixture design applied for the development of films based on starch, polyvinyl alcohol, and glycerol J Zanela, JB Olivato, AP Dias, MVE Grossmann, F Yamashita Journal of Applied Polymer Science 132 (43) , 2015 2015 Citations: 42
Starch/polyester films: simultaneous optimisation of the properties for the production of biodegradable plastic bags JB Olivato, MVE Grossmann, AP Bilck, F Yamashita, LM Oliveira Polímeros 23 (1), 32-36 , 2013 2013 Citations: 40
Effects of the incorporation of saturated fatty acids on the mechanical and barrier properties of biodegradable films MM Nobrega, JB Olivato, MVE Grossmann, E Bona, F Yamashita Journal of Applied Polymer Science 124 (5), 3695-3703 , 2012 2012 Citations: 39
Antimicrobial dressing of silver sulfadiazine-loaded halloysite/cassava starch-based (bio) nanocomposites GLP da Silva, LCA Morais, JB Olivato, J Marini, PC Ferrari Journal of biomaterials applications 35 (9), 1096-1108 , 2021 2021 Citations: 32
Poly (lactic acid)/thermoplastic starch sheets: effect of adipate esters on the morphological, mechanical and barrier properties MA Shirai, JB Olivato, IM Demiate, CMO Müller, MVE Grossmann, ... Polímeros 26 (1), 66-73 , 2016 2016 Citations: 30
Study of the compatibilizer effect in the properties of starch/polyester blends JB Olivato, CMO Müller, F Yamashita, MVE Grossmann, MM Nobrega Polímeros 23 (3), 346-351 , 2013 2013 Citations: 28