Jeevan Jyoti Kaushik
@sgrru.in
Associate Professor, Department of Microbiology
Shri Guru Ram Rai University, Dehradun Uttarakhand
RESEARCH, TEACHING, or OTHER INTERESTS
Molecular Biology, Microbiology, Cell Biology, General Immunology and Microbiology
Scopus Publications
Scopus Publications
Jeevan Jyoti Kaushik, Atul Kaushik, Dawit Mebrahtu, Efrem Bereket, Elen Eyob, Hana Kelifa, Kokob Weldemichael, and Rahwa Andebrhan
Springer Science and Business Media LLC
Filmon Kiflezghi Kiflemariam, Abiel Ghebrehiwet Tewelde, Ali Mahmud Hamid, Bilal Mussa Beshir, Samrawit Negasi Solomon, Tesfu Gonets Eman, Daniel Mebrahtu Abraha, Russom Kahsu, John Issac, and Jeevan Jyoti Kaushik
Informa UK Limited
Background Currently, cardiovascular disorders are the primary cause of mortality in the world and constitute a serious medical problem. Blood coagulation is an essential process to prevent excessive blood loss through injured blood vessels; however, abnormal blood clotting in the blood vessels can result in fatal cardiovascular disorders. This study investigated the in vitro anticoagulant activity of Meriandra dianthera crude extract and its fractions and their erythrocyte membrane stabilizing activity. Methods The plant leaves were extracted by a decoction method and were further fractionated by a liquid–liquid partition with a solvent of crescent polarity. The in vitro anticoagulant activity of the plant extract and its fractions was assessed by PT and APTT assays, while the membrane stabilizing activity was determined through hypotonic induced hemolysis. Results The crude aqueous leaf extract of Meriandra dianthera significantly (P < 0.001) prolonged the intrinsic clotting pathway measured by APTT by specifically acting on the intrinsic coagulation pathway. By using liquid–liquid fractionation, the residual aqueous fraction was identified as the fraction responsible for the anticoagulant activity of the crude extract as it significantly (P<0.001) prolonged APTT while the other fractions failed. Both the crude extract and its aqueous residue fraction did not affect the extrinsic coagulation pathway measured by PT. In the membrane stabilizing assay, crude extract and aqueous residue fraction showed the highest membrane stabilizing activity. Conclusion The crude extract and its aqueous residue fraction showed a potent in vitro anticoagulant and membrane stabilizing activity, which shows the potential of the plant’s leaves as a new source of bioactive molecules for coagulation-related disorders.
Atul Kaushik, Anghesom Ambesajir, and Jeevan Kaushik
Bentham Science Publishers Ltd.
Anghesom Ambesajir, Atul Kaushik, Jeevan J. Kaushik, and Sham T. Petros
Elsevier BV
V. Parcha, A. Kaushik, J. Kaushik, M.S.M. Rawat, and S.A.S. Biswas
Informa UK Limited
Abstract The current study was conducted to investigate antibacterial activity of Alseodaphne andersonii. (King ex Hook. f.) Kosterm. (Lauraceae) leaf extracts against 15 strains of pathogenic bacteria. Inhibition of bacterial growth was investigated using disk diffusion methods. Methanol leaf extract of Alseodaphne andersonii. was active against all assayed bacteria. The minimal inhibitory concentration (MIC) values and minimal bactericidal concentration (MBC) values were also determined. Results indicated that Alseodaphne andersonii. extracts must possess major antibacterial components against infectious microorganisms. The MIC and MBC of methanol extract of leaves against Staphylococcus aureus. were found to be 0.414 and 6.62 mg/mL, and for Staphylococcus epidermidis. 0.207 and 3.31 mg/mL, respectively. Alseodaphne andersonii. might provide promising therapeutic agents against infections with bacterial organisms. This is the first report about the antimicrobial activity of Alseodaphne andersonii.. The plant is reported to be used as timber only.