Juliana

Juliana has worked in SSR primer development, phylogeny, genetic diversity, and gene expression analysis using RT-PCR, and RNA-seq. She has published 10 papers, 5 as first author. She integrated 7 project teams. She has acted as a reviewer in the Molecular Biology Reports journal and the South African Journal of Botany. Juliana has experience in Bioinformatics, Biotechnology, Genetics, Evolution, and Biodiversity. Currently, she has a post-doc position at UTAD and leads a BioISI project.

EDUCATION

Juliana Lopes has PhD in Biological Science in 2019 at Universidade Federal de Juiz de Fora, with an exchange period at the University of Florida under Dr. Pamela Soltis's supervision. She has a Master's and bachelor's Degree in Biological Science from the same institution.

RESEARCH, TEACHING, or OTHER INTERESTS

Plant Science, Molecular Biology, Genetics, Computer Science Applications

Scopus Publications

Phenotypic variation seems not to be associated with the genetic profile in Zygopetalum (Orchidaceae): a case study of a high-elevation rocky complex
Shaiany Sabrina Lopes Gomes, Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Elisa Guimarães Cabral, Ana Luisa Sousa Azevedo, Marco Antonio Machado, José Marcello Salabert de Campos, Luiz Menini Neto, and Lyderson Facio Viccini
Springer Science and Business Media LLC



Water stress modulates terpene biosynthesis and morphophysiology at different ploidal levels in Lippia alba (Mill.) N. E. Brown (Verbenaceae)
Juliana Mainenti Leal Lopes, Laís Stehling de Queiroz Nascimento, Vinicius Carius Souza, Elyabe Monteiro de Matos, Evandro Alexandre Fortini, Richard Michael Grazul, Marcelo Oliveira Santos, Douglas E. Soltis, Pamela S. Soltis, Wagner Campos Otoni,et al.
Springer Science and Business Media LLC



Enhancing the taxonomic delimitation of Lippia origanoides Kunth (Verbenaceae) by analyzing volatile terpenes and molecular markers in micropropagated accessions
Laís M. Frattini, Arthur A. Oliveira, Jhoão S. Kotsubo, Íris B. Gorne, Isabel C. Silva, Jober C. E. Freitas, Cristiano F. Resende, Ana C. Mezzonato-Pires, Elyabe M. Matos, Juliana M. L. Lopes,et al.
Springer Science and Business Media LLC



Evaluation of reference genes for quantitative analysis of gene expression in Lippia alba under abiotic stress
Laís Stehling de Queiroz Nascimento, Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Vinicius Carius Souza, Diego Silva Batista, Marcelo de Oliveira Santos, Wagner Campos Otoni, and Lyderson Facio Viccini
Springer Science and Business Media LLC



Chromosomal aberrations and changes in the methylation patterns of Lactuca sativa L. (Asteraceae) exposed to carbon nanotubes
Juliana Tatiara da Costa Siqueira, Aryane Campos Reis, Juliana Mainenti Leal Lopes, Luiz Orlando Ladeira, Lyderson Facio Viccini, Humberto de Mello Brandão, Michele Munk, and Saulo Marçal de Sousa
Springer Science and Business Media LLC



Aneuploids and its increment on diversity of Lippia alba polyploid complex: genetic aspects and origin
Juliana Mainenti Leal Lopes, Victória Rabelo Campos, Aryane Campos Reis, Elyabe Monteiro de Matos, Ana Luisa Sousa Azevedo, Marco Antonio Machado, Richard Michael Grazul, and Lyderson Facio Viccini
Springer Science and Business Media LLC



Deciphering ploidal levels of Lippia alba by using proteomics
Camila Maurmann de Souza, Cristiane Zorzatto, Carla G.S. Quinhones, Juliana Mainenti Leal Lopes, Humberto Henrique de Carvalho, Wagner L. Araújo, and Lyderson Facio Viccini
Elsevier BV



Validation of reference genes for quantitative gene expression in the Lippia alba polyploid complex (Verbenaceae)
Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Laís Stehling de Queiroz Nascimento, and Lyderson Facio Viccini
Springer Science and Business Media LLC



Development of microsatellite markers for Lippia alba and related Lippia species
Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Cristiane Zorzatto, Ana Luisa Sousa Azevedo, Marco Antonio Machado, Michael Chester, and Lyderson Facio Viccini
Springer Science and Business Media LLC



Induction of Synthetic Polyploids and Assessment of Genomic Stability in Lippia alba
Sirlei Aparecida Julião, Christiane do Valle Ribeiro, Juliana Mainenti Leal Lopes, Elyabe Monteiro de Matos, Aryane Campos Reis, Paulo Henrique Pereira Peixoto, Marco Antonio Machado, Ana Luisa Sousa Azevedo, Richard Michael Grazul, José Marcello Salabert de Campos,et al.
Frontiers Media SA
Polyploidy is widely recognized as a major evolutionary force in plants and has been reported in the genus Lippia (Verbenaceae). Lippia alba, the most studied species, has been documented as a polyploid complex involving at least four ploidal levels. L. alba presents remarkable chemical and genetic variation and represents a model for understanding genome organization. Although the economic and medicinal importance of the species has been widely described, no established polyploid induction protocol has been reported so far. Here, we describe the production of synthetic polyploid plants of L. alba using colchicine. The ploidal levels were estimated by flow cytometry and chromosome counting. In addition, FISH and molecular markers approaches were used to confirm the stability of the synthetic polyploids. The major component of the essential oils was estimated by GCMS to compare with the natural individuals. Tetraploids and triploids were produced providing new opportunities for investigating medicinal, pharmacological, and economic applications as well as addressing intrinsic questions involved in the polyploidization process in tropical plants.


Genetic relationships and polyploid origins in the Lippia alba complex
Juliana M. L. Lopes, Humberto Henrique de Carvalho, Cristiane Zorzatto, Ana Luisa S. Azevedo, Marco Antonio Machado, Fátima Regina G. Salimena, Richard M. Grazul, Matthew A. Gitzendanner, Douglas E. Soltis, Pamela S. Soltis,et al.
Wiley
PremisePlant genomes vary in size and complexity due in part to polyploidization. Latitudinal analyses of polyploidy are biased toward floras of temperate regions, with much less research done in the tropics. Lippia alba has been described as a tropical polyploid complex with diploid, triploid, tetraploid, and hexaploid accessions. However, no data regarding relationships among the ploidal levels and their origins have been reported. Our goals are to clarify the relationships among accessions of Lippia alba and the origins of each ploidal level.MethodsWe investigated 98 samples representing all five geographical regions of Brazil and all ploidal levels using microsatellite (SSR) allelic variation and DNA sequences of ITS and trnL‐F. Nine morphological structures were analyzed from 33 herbarium samples, and the chemical compounds of 78 accessions were analyzed by GC–MS.ResultsGenetic distance analysis, the alignment block pattern, as well as RAxML and Bayesian trees showed that accessions grouped by ploidal level. The triploids form a well‐defined group that originated from a single group of diploids. The tetraploids and hexaploid grouped together in SSR and trnL‐F analyses. The recovered groups agree with chemical data and morphology.ConclusionsThe accessions grouped by ploidal level. Only one origin of triploids from a single group of diploids was observed. The tetraploid origin is uncertain; however, it appears to have contributed to the origin of the hexaploid. This framework reveals linkages among the ploidal levels, providing new insights into the evolution of a polyploid complex of tropical plants.