I Nengah Kerta Besung
@unud.ac.id
Scopus Publications
Scopus Publications
N.M.A. Tarini, N.M. Susilawathi, A.A.R. Sudewi, A. Soejitno, N.N.D. Fatmawati, I.P.B. Mayura, A.A.W. Lestari, G. Suputra, I.K. Subrata, C.I.S.D. Astiti,et al.
Elsevier BV
Kadek Karang Agustina, Made Suma Anthara, Nengah Anom Adi Nugraha Sibang, Wayan Adi Rinta Wiguna, Jendra Krisna Apramada, Wayan Nico Fajar Gunawan, Ida Bagus Made Oka, Made Subrata, and Nengah Kerta Besung
Veterinary World
Background and Aim: Several free-roaming dogs can be easily found in the public areas of Bali. They go out in search of foods and friends and defecate everywhere. In general, these groups of dogs do not receive good healthcare from their owners and are generally threatened by some disease-causing organisms, especially helminths. This study was conducted to identify and measure the prevalence of soil-transmitted helminths (STHs) that cause infection in free-roaming dogs in Bali Province, Indonesia. Materials and Methods: A total of 1611 fresh dog fecal samples were collected from all areas of Bali Province and subjected to qualitative fecal examination using flotation techniques to obtain STH eggs. Results: The incidence of STHs in free-roaming dogs was 38.36%. However, three types of STHs were identified, including Ancylostoma spp., Ascaris spp., and Trichuris spp. Ancylostoma spp. had the highest prevalence of 37.8%, followed by Ascaris spp. and Trichuris spp. at 6.02% and 0.87%, respectively. Multiple infections of these worms were also recorded. The polyparasitism prevalence of Ancylostoma spp. and Ascaris spp. was 3.85%, followed by that of Ancylostoma spp. and Trichuris spp. at 0.5% and that of Ascaris spp. and Trichuris spp. at 0.06%. There were no altitude-wise differences in the prevalence of STH infection. Conclusion: The prevalence of STHs was high in free-roaming dogs. This finding necessitates more serious attention as it affects both animal and public health.
I. K. Narcana, I. W. Suardana, and I. N. K. Besung
Veterinary World
Aim: This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province. Materials and Methods: The isolates of P. multocida stored in frozen storage were cultured in blood agar as a selective medium and identified conventionally. Molecular tests were initiated by DNA isolation and then followed by polymerase chain reaction tests with specific primers for the determination of P. multocida serotype A or B. Positive strain of serotype B was then confirmed molecularly using 16S rRNA gene primer and followed by the sequencing of nucleotides. Results: The study showed that both P. multocida isolates from Sumba island, i.e. PM1 is isolated from East Sumba district, while PM2 isolated from West Sumba district have 99.6% homology. Both isolates also known have 99% similarities with P. multocida originated from India, Britain, and Japan, respectively. The isolates share the same clade in the phylogenetic tree. Conclusion: The 16S rRNA sequencing revealed a high similarity of P. multocida serotype B:2 isolated from Sumba island with the Indian isolates although the sample size is very small. Therefore, further molecular studies like multilocus sequence typing, VNTR need to be performed using a larger number of samples to establish the genetic relatedness observed in this study.
I Nengah Kerta Besung, I Gusti Ketut Suarjana, Kadek Karang Agustina, Ida Bagus Oka Winaya, Hamong Soeharsono, Ni Ketut Suwiti, and Gusti Ngurah Mahardika
Springer Science and Business Media LLC
Abstract Objective Streptococcus suis (S. suis) is a causative agent for various syndromes in pigs. It can be transmitted to humans with typical symptoms of meningitis and death. Although human infections have been confirmed at Bali Referral Hospital, Indonesia, since 2014, the bacteria have not been isolated from pigs. Here, we provide confirmation of the presence of the bacteria in sick pigs in the province. Results Streptococcus suis was confirmed in 8 of 30 cases. The final confirmation was made using PCR and sequencing of the glutamate dehydrogenase (GDH) and recombination/repair protein (recN) gene fragments. Upon PCR serotyping, two were confirmed to be serotype 2 or 1/2. Prominent histopathological lesions of confirmed cases were meningitis, endocarditis, pericarditis, bronchopneumonia, enteritis and glomerulonephritis. The dominant inflammatory cells were neutrophils and macrophages. Further research is needed to understand the risk factors for human infection. Community awareness on the risk of contracting S. suis and vaccine development are needed to prevent human infections.
I Nengah Kerta Besung, NI LUH WATINIASIH, GUSTI NGURAH KADE MAHARDIKA, KADEK KARANG AGUSTINA, and NI KETUT SUWITI
UNS Solo
Abstract. Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of Bali cattle (Bos javanicus) from four different types of land in different rearing areas in Bali, Nusa Penida, and Sumbawa Islands (Indonesia). Biodiversitas 20: 2931-2936. This study aims to prove that the mineral level of Bali cattle in serum is dependent on the origin and raising habitat. Serum samples were collected from three islands in Indonesia, i.e. Bali, Nusa Penida, and Sumbawa. Samples from Bali were further classified into four different areas (i.e. agroforestry, perennial plantation, horticultural plantation, and rice field). Blood samples were collected in the jugular vein without anticoagulant. The contents of macrominerals (Ca, Mg, Na, K, P) and microminerals (Fe, Cu, Zn, Co, Mn) were measured using the flame method in atomic absorption spectrophotometer. The results showed that the level of phosphor (P) macromineral and all microminerals under study were influenced by the origin of cattle. The macrominerals are statistically varied in different cattle raising environments. The same is also valid for all microminerals, with an exception of Fe. All mineral levels under study were statistically equal in male and female Bali cattle. Phosphor macromineral and all microminerals levels were origin and environment-dependent. Supplementation of deficient minerals in a certain area and land type is recommended to improve the performance of Bali cattle.
N. K. Suwiti, I N. K. Besung, and G. N. Mahardika
Veterinary World
Aim: Bali cattle (Bos javanicus) are an Indonesian’s native cattle breed that distributed in Asia to Australia. The scientific literature on these cattle is scarce. The growth hormone (GH) of Bali cattle is investigated from three separated islands, namely, Bali, Nusa Penida, and Sumbawa. Materials and Methods: Forty plasma samples were collected from each island, and the GH was measured using a commercial enzyme-linked immunosorbent assay kit. The data were analyzed based on the origin, sex, and cattle raising practices. Results: We found that the GH level (bovine GH [BGH]) of animal kept in stall 1.72±0.70 µg/ml was higher than free-grazing animal 1.27±0.81 µg/ml. The GH level was lower in female (1.22±0.62 µg/ml) compared to male animals (1.77±0.83 µg/ml). Conclusion: We conclude that the level of BGH in Bali cattle was low and statistically equal from all origins. The different level was related to sex and management practices. Further validation is needed through observing the growth rate following BGH administration and discovering the inbreeding coefficient of the animal in Indonesia.