The potential of mollusc-associated fungi as anti-pathogens Josua Gaol, Delianis Pringgenies, Wilis Setyati, Sunelsya Surya, Muhammad Zainuddin, et al. Open Veterinary Journal, 2026 Background: Molluscs are invertebrate organisms known to produce secondary metabolites that help them adapt to their environment. Aim: This study aims to explore the potential of fungi associated with molluscs and evaluate their secondary metabolites potential antimicrobial agents against human pathogens. Methods: The fungi isolated from molluscs were examined both macroscopically and microscopically. Promising isolated were selected and cultured in bulk for phytochemical screening and thin-layer chromatography (TLC) analysis. Extract from these fungal tested at a concentration 1000 μg/disc. Observations recorded every 12 hours over a 36-periods. Molecular identification of the selected isolated was performed using the Quick-DNA Magbead Plus Kit (Zymo Research, D4082). Statistical analysis was used to determine the significant differences isolates and control (p <0.05). Results: The extract from fungal isolate G1T4 demonstrated both antibacterial and antifungal activity against all four test pathogens, as evidenced by the formation of clear inhibition zones. Extracts from isolates B3M6, B4T6, and B2L4 showed antibacterial effect against Staphylococcus aureus, Escherichia coli, and the test fungus Candida albicans, but exhibited no activity against the fungal pathogen Trichoderma harzianum. The presence of inhibition zones suggests that these extracts contain active secondary metabolites or active compounds in the associated fungus extract. The result of statistical analysis showed significant differences (p < 0.05) between isolates and controls at all observation times (12 – 72 hours) against Escherichia coli and Staphylococcus aureus, Candida albicans and Trichoderma harzianum showed GT14 are consistently higher than other isolates (p <0.05). Conclusion: Molecular analysis revealed that isolate G1T4 is closely related to Aspergillus subramanianii (accession number PQ325939), while isolate B3M6 is closely related to Cladosporium halotolerans (accession number PQ325940).
Enzymes from mollusk-associated bacteria in Ambon waters of Wallacea: Prospective cosmetic applications Sunelsya Surya, Delianis Pringgenies, Yashwant Pathak, Sri Sedjati, Tatas Brotosudarmo, et al. Open Veterinary Journal, 2026 Background: Marine mollusks serve as hosts for symbiotic bacteria capable of producing secondary metabolites with significant biotechnological potential, including those used in the cosmetic industry. The rich aquatic biodiversity of Ambon, located within the Wallacea region, presents a unique opportunity to explore marine microbes with distinctive metabolite profiles. However, research exploring mollusk symbiont bacteria from Ambon for cosmetic-related bioactive metabolites remains scarce. The valuable metabolite-producing potential of these compounds highlights the importance of further investigating their biological activities and metabolite profiles for prospective cosmetic applications. Aim: This study aimed to evaluate the mollusk-associated bacteria for antibacterial and enzymatic activities, identify the bacterial strains, and analyze their secondary metabolites using GC-MS to support the development of active cosmetic ingredients. Methods: As many as 6 mollusks were selectively collected from Sopapey waters, Ambon region, and bacterial isolates were obtained using standard culturing techniques. Antibacterial activity was evaluated against Cutibacterium acnes and Staphylococcus epidermidis, enzymatic activities were tested using 1% skim milk on agar medium, and molecular identification was conducted using 16S rRNA sequencing. GC-MS analysis was employed to profile secondary metabolites from selected isolates. Statistical analysis was performed using one-way analysis of variance in RStudio to determine significant differences among the isolates and controls. Results: Three isolates exhibited inhibitory activity, with SPG241 showing the strongest inhibition zone, 4.80 ± 0.57 mm (24 hours) and 2.75 ± 0.49 mm (48 hours) against C. acnes and 8.43 ± 1.26 mm (24 hours) and 7.04 ± 0.97 mm (48 hours) against S. epidermidis. Protease assays demonstrated that eight isolates possessed proteolytic activity, with hydrolysis zones of 11.65–13.85 mm in 48 hours. Molecular identification revealed that the potential isolates belonged to Pseudoalteromonas citrea, Pseudoalteromonas peptidolytica, Vibrio xuii, and Vibrio tubiashii. These symbionts produced bioactive metabolites, including esters (e.g., methyl palmitate and trans-13-octadecenoic acid) and functional molecules, such as 2,3-butanediol and 2-piperidinone. Isolates with the highest enzymatic activity exhibited promising cosmetic properties, including emollient, humectant, antioxidant, anti-inflammatory, and antibacterial effects. Conclusion: The findings confirm that mollusk symbiont bacteria from Sopapey water, particularly within the Wallacea region, are a promising source of secondary metabolites for natural-based cosmetic products, reinforcing the region’s importance as a promising frontier for the discovery of innovative marine bioactive compounds.
A New Record of Rochia maxima (Koch, 1844) Through a Species Clarification of Lola Snail, Bangka Belitung Islands, Indonesia Siti Aisyah, Delianis Pringgenies, Okto Supratman, Dimas Wahyudi, Fahmi Rizal, et al. Jurnal Ilmiah Perikanan Dan Kelautan, 2025 Graphical Abstract Highlight Research Lola snails originating from the Bangka Belitung Islands (Nasik Strait, Rebo Waters, and Ketawai Island) were identified as a maxima not to be R. nilotica species based on morphological molecular identification. Maxima and R. nilotica have the same morphological form, but they are genetically different. The clarification of the Lola snail species in this study serves as a reference in determining the conservation status of the Lola snail. The clarification of the Lola snail species serves as a reference in determining the catch quota from the Bangka Belitung Islands. Abstract The use of Lola snails (Rochia maxima) in the Bangka Belitung has continued to increase. However, research focused on Lola snails originating from the Bangka Belitung has not been thoroughly conducted. In a previous study, the Lola snail sample found in the Bangka Belitung was Trochus niloticus (current name: Rochia nilotica). This study aimed to identify Lola snail species using the DNA barcoding as a way of clarifying Lola snail species that originated in the Bangka Belitung. Lola snail sampling was carried out at three locations, which were Nasik Strait, Ketawai, and Rebo Waters. This study was conducted through five stages, including Lola snail tissue sampling, DNA extraction, amplification by PCR, sequencing, and data analysis. The results showed that the molecular identification of the Cytochrome Oxidase Subunit 1 (COI) gene in Lola snail samples was identified as Rochia maxima species (Koch, 1844). The results of the phylogenetic tree analysis showed that Lola snails found in the Bangka Belitung were close to Tectus maximus (or Rochia maxima) species. R. maxima has a lighter shell than R. nilotica, a clear conical shape, and a circle on the body that does not widen at the edges. Unlike zoologists who said that R. maxima is the primitive form of R. nilotica, the present study found that they have the same morphology and are considered similar but genetically different. The clarification of Lola snail species can be used to determine the conservation status and catching quota of Lola snails from the Bangka Belitung.
Nutrient dynamic in sediment and organs of the mangrove Avicennia marina (Forsk.) Vierh Dafit Ariyanto, Delianis Pringgenies, Devica Natalia Br Ginting Bio Web of Conferences, 2025 Avicennia marina is a mangrove species that can be found on the coast of Indonesia, specifically in Lampung province. The aim of this study was to investigate the relationship between Avicennia marina nutrients in natural mangroves. This study was conducted in the coast of Pesawaran, Lampung, Indonesia in December 2023. Nutrient sampling included leaves, roots, and sediment in the Avicennia marina mangrove area. The statistical analysis used the principal component analysis (PCA) approach. The nutrients analyzed were nitrogen (N), phosphorus (P), zinc (Z), magnesium (Mg), and iron (Fe) in the leaves, roots, and sediment. The results of this study revealed the eigenvalues and loading factors of the nutrient variables. The main factor (F1) presented 99.78% of the variable data where each variables contributed not significantly different. The main to the least variables were Mg (0.47), Fe (-0.46), N (0.43), Zn (-0.43), and P (0.42). The negative values of Fe and Zn variables indicated a negative correlation with Mg, N, and P. If the Fe and Zn values increase, then the Mg, N, and P values will be the opposite.
Characterization of Antibacterial Compounds from Seaweed Against Pathogenic Bacteria Wilis Ari Setyati, AB Susanto, Dony Bayu Putra Pamungkas, Dewi Basthika Makrima, Juwita Lesly Senduk, et al. Polish Journal of Environmental Studies, 2025 Bacterial communities collaborating with seaweed play an important role in many aspects, especially in the production of secondary metabolites that help ecosystem function and have good prospects for pharmacological utilization. The objective of this study was to determine the antibacterial activity of associated bacteria isolated from seaweed in Awur Bay, Jepara, Indonesia. The antibacterial test uses the pathogenic bacteria Staphylococcus aureus , Pseudomonas aeruginosa , and Micrococcus luteus . Isolation of seaweed-associated bacteria obtained 4 potential isolates, which were closely related to Vibrionaceae bacterium PH25 (99.86%), Vibrio alginolyticus strain GS MYPK1 (99.65%), Salinivibrio costicola strain M318 (99.86%), and Vibrio alginolyticu s strain 2014V-1011 (99.93%). In disc diffusion, MIC, and MBC tests, four isolates showed potential antibacterial activity. TLC identified groups of compounds that were reported to have antibacterial activity, such as alkaloids, terpenoids, steroids, and flavonoids . Compounds that were reported to have antibacterial properties were identified from GCMS, one of which was the compound azulane (CAS).
The impact of mixed feed of seaweed Gracilaria sp. and transfer factor formula on the immune response of Nile tilapia (Oreochromis niloticus) Aacl Bioflux, 2023
Enzymes from mollusk-associated bacteria in Ambon waters of Wallacea: Prospective cosmetic applications S Surya, D Pringgenies, Y Pathak, S Sedjati, THP Brotosudarmo, ... Open Veterinary Journal 16 (3), 1704-1704 , 2026 2026
Bacterial presence and nutrient dynamics in mangrove crab mounds: links to blue carbon revealed by molecular approaches D Pringgenies, WA Setyati, D Ariyanto Estuarine, Coastal and Shelf Science, 109790 , 2026 2026
The potential of mollusc-associated fungi as anti-pathogens JGL Gaol, D Pringgenies, WA Setyati, S Surya, M Zainuddin, D Ariyanto Open Veterinary Journal 16 (1), 626-626 , 2026 2026
Exploration of Secondary Metabolites from Mangroves and their Simbionts on the Merauke Coast for Innovations in Medicine and Cosmetics D Pringgenies, Y Mulyani, EHP Melmambessy, A Nuskiya, M Nurhasanah, ... International Journal of Agriculture and Biosciences 15 (4), 1794-1805 , 2026 2026
Erratum to “Phytochemicals and Antioxidant Activity of Microalgae Dunaliella salina and Botryococcus braunii”[Jurnal Ilmiah Perikanan dan Kelautan Volume 15 Issue 2, 16 Agustus … A Ridlo, D Pringgenies 2026
Detecting Cold Seawater Upwelling in Pantar Strait via Multi-Source Satellite Data Integration and Assessing Its Ecological Impacts A Hartoko, JF Maro, M Helmi, HAZ Syafina, AR Ashari, D Ariyanto International Journal of Design and Nature and Ecodynamics 20 (9), 2011-2019 , 2025 2025
Characterization of Antibacterial Compounds from Seaweed Against Pathogenic Bacteria. WA Setyati, AB Susanto, DBP Pamungkas, DB Makrima, JL Senduk, ... Polish Journal of Environmental Studies 34 (4) , 2025 2025 Citations: 2
Antioxidant, Antibacterial, and Antifungal Characteristics on Crude Collagen Extracts of Sea Cucumber (Stichopus hermanii) R Pramesti, WA Setyati, N Soenardjo, D Ariyanto International Journal of Design and Nature and Ecodynamics 20 (3), 523-528 , 2025 2025 Citations: 2
Nutrient dynamic in sediment and organs of the mangrove Avicennia marina (Forsk.) Vierh D Ariyanto, D Pringgenies, D Natalia Br Ginting BIO Web of Conferences 156, 02012 , 2025 2025 Citations: 1
A New Record of Rochia maxima (Koch, 1844) Through a Species Clarification of Lola Snail, Bangka Belitung Islands, Indonesia S Aisyah, D Pringgenies, O Supratman, D Wahyudi, F Rizal, D Ariyanto, ... Jurnal Ilmiah Perikanan dan Kelautan 17 (1), 16 , 2025 2025 Citations: 1
Carbon sequestration rate in sediment mangroves from natural and rehabilitated mangroves D Ariyanto, D Pringgenies Global Journal of Environmental Science and Management 10 (4), 1951-1960 , 2024 2024 Citations: 8
Structural equation modeling for social capital empowerment in supporting mangrove rehabilitation I Listiana, D Ariyanto Global Journal of Environmental Science and Management 10 (4), 2031-2044 , 2024 2024 Citations: 2
Natural mangrove associated gastropods at Bengkalis island, Riau Province, Indonesia: prelimanary survey D Ariyanto, D Pringgenies IOP Conference Series: Earth and Environmental Science 1356 (1), 012045 , 2024 2024 Citations: 1
Hair cosmetic ingredients from the symbiont bacteria Xylocarpus sp. fruit are based on antimicrobial and antifungal bacteria D Pringgenies, WA Setyati, A Ismail, D Ariyanto Journal of Applied Pharmaceutical Science 14 (5), 211-219 , 2024 2024 Citations: 4
The potential two types of green macroalgae (Caulerpa racemosa and Caulerpa lentillifera) as a natural food preservative from Jepara beach, Indonesia GW Santosa, A Djunaedi, AB Susanto, D Pringgenies, D Ariyanto, ... Trends in Sciences 21 (5), 7394-7394 , 2024 2024 Citations: 10
Online First D Pringgenies, WA Setyati, A Ismail, D Ariyanto 2024
Bittern as an Anti-Inflammatory on the Skin of Swiss Strain Mice D Pringgenies, A Ridlo, N Alifia, D Ariyanto ASM Science Journal 19, 1-8 , 2024 2024
Nutrient Distribution in Natural and Rehabilitated Mangroves with Various Types of Mangroves, Lampung, Indonesia D Ariyanto, D Pringgenies International Journal of Design and Nature and Ecodynamics 19 (6), 2171 - 2178 , 2024 2024 Citations: 6
Oceanographic variables and stomach content of stranded whale shark (Rhincodon typus) on the South Java Coast Hartoko A., Pringgenies D., Helmi M., Aisyah S., Ariyanto D., Atmaja E. J. J. AACL Bioflux 17 (1), 382- 393 , 2024 2024
Enhancing coastal community participation in mangrove rehabilitation through structural equation modeling I Listiana Global Journal of Environmental Science and Management 10 (2), 873-890 , 2024 2024 Citations: 9
MOST CITED SCHOLAR PUBLICATIONS
Hartoko, A; Suryanti; Chayaningrum, S; Febrianti, DA; Ariyanto, D. 2014. Carbon biomass algorithms development for mangrove vegetation in Kemujan, Parang Island Karimunjawa … Procedia Environ. Sci 23, 39-47 , 0 Citations: 48
The association of Cassidula nucleus (Gmelin 1791) and Cassidula angulifera (Petit 1841) with mangrove in Banggi Coast, Central Java, Indonesia Ariyanto D., Bengen D. G., Prartono T., Wardiatno Y. AACL Bioflux 11 (2), 348-361 , 2018 2018.0 Citations: 43
The relationship between content of particular metabolites of fallen mangrove leaves and the rate at which the leaves decompose over time YW Dafit Ariyanto, Dietriech G. Bengen, Tri Prartono Biodiversitas 19 (3), 700-7005 , 2018 2018.0 Citations: 36
The physicochemical factors and litter dynamics (Rhizophora mucronata lam. and Rhizophora stylosa griff) of replanted Mangroves, Rembang, Central Java, Indonesia D Ariyanto, DG Bengen, T Prartono, Y Wardiatno Environment and Natural Resources Journal 17 (4), 11-19 , 2019 2019.0 Citations: 33
FOOD PREFERENCE ON TELESCOPIUM TELESCOPIUM (MOLLUSCA : GASTROPODA) BASED ON FOOD SOURCES IN MANGROVE ECOSYSTEM D Ariyanto Plant Archives 19 (1), 913-916 , 2019 2019.0 Citations: 32
Exploration of antimicrobial potency of mangrove symbiont against multi-drug resistant bacteria D Pringgenies, WA Setyati, A Djunaedi, R Pramesti, S Rudiyanti, ... Jurnal Ilmiah Perikanan dan Kelautan 13 (2), 222-232 , 2021 2021.0 Citations: 30
The amino acid contents in mangrove Rhizophora mucronata leaves in Asahan, North Sumatra, Indonesia H Hamim E3S Web of Conferences , 2020 2020.0 Citations: 27
The antibacterial and antifungal potential of marine natural ingredients from the symbiont bacteria of mangrove. D Pringgenies, WA Setyati, F Feliatra, D Ariyanto 2023.0 Citations: 24
Distribution and abundance of Cerithideopsilla djadjariensis (Martin 1899)(potamididae) on Avicennia marina in Rembang, Central Java, Indonesia D Ariyanto, D G Bengen, T Prartono, Y Wardiatno Egyptian Journal of Aquatic Biology and Fisheries 24 (3), 323-332 , 2020 2020.0 Citations: 24
The differences of the elements content in Rhizophora mucronata leaves from Asahan Regency, North Sumatra, Indonesia D Ariyanto, H Gunawan, D Puspitasari, SS Ningsih, A Jayanegara, ... Pol. J. Nat. Sci 34 (4), 481-491 , 2019 2019.0 Citations: 22
Hamim. 2019. Identification of the chemical profile of Rhizophora mucronata mangrove green leaves from the eastern coast OfAsahan, North Sumatra, Indonesia D Ariyanto, H Gunawan, D Puspitasari, SS Ningsih, A Jayanegara Plant Archives 19 (2), 4045-4049 , 0 Citations: 19
Characteristics of bioactive compounds of Holothuria atra (Jaeger, 1833) associated bacteria GW Santosa, A Djunaedi, AB Susanto, D Pringgenies, D Ariyanto Aquaculture, Aquarium, Conservation & Legislation 13 (4), 2161-2169 , 2020 2020.0 Citations: 17
Symbiotic microbes from various seaweeds with antimicrobial and fermentative properties D Pringgenies, EI Retnowati, D Ariyanto, K Dewi, MAS Viharyo, ... Aquaculture, Aquarium, Conservation & Legislation 13 (4), 2211-2217 , 2020 2020.0 Citations: 15
Productivity and CNP availability in Rhizophora apiculata Blume and Avicennia marina (Forssk.) Vierh. at Banggi Coast, Central Java-Indonesia D Ariyanto, DG Bengen, T Prartono, Y Wardiatno Advances in Environmental Sciences 10 (3), 137 , 2018 2018.0 Citations: 14
Distribution Of Batillaria Zonalis (Mollusca: Gastropoda) on Avicennia Marina (Forsk.) Vierh In The Coast Of Banggi, Rembang, Central Java D Ariyanto, DG Bengen, T Prartono, Y Wardiatno Omni-Akuatika 14 (3), 10-17 , 2018 2018.0 Citations: 14
Bakteri Konsorsium Dari Serasah Mangrove Untuk Produksi Kompos' D Pringgenies, R Widiyadmi, D Ariyanto, R Idris, A Djunaedi Jurnal Pengelolaan Perairan 1 (2), 19-26 , 2018 2018.0 Citations: 14
Heavy Metal (Pb) in the Rhizophora apiculata Mangrove in Asahan, North Sumatera, Indonesia D Ariyanto, H Gunawan, DW Purba International Seminar on Promoting Local Resources for Sustainable … , 2021 2021.0 Citations: 13
Length-Weight relationships and condition factors of Telescopium telescopium (Gastropoda: Potamididae) in banggi coast of central Java, Java Island, Indonesia D Ariyanto International Journal of Fisheries and Aquatic Studies 6 (2), 548-550 , 2018 2018.0 Citations: 13
Pemanfaatan Lahan Pekarangan dengan Sistem Aquaponik dalam Menunjang Perekonomian di Desa Sungai Lama, Kabupaten Asahan, Sumatera Utara D Puspitasari, D Ariyanto, A Rodiansah, I Zahar Jurnal Anadara Pengabdian Kepada Masyarakat 2 (1), 67-71 , 2020 2020.0 Citations: 12
Stomata Dynamic on All types of Mangrove in Rembang District, Central Java, Indonesia D Ariyanto International Journal of Sciences: Basic and Applied Research (IJSBAR) 38 (1 … , 2018 2018.0 Citations: 12