Ki-Taek Lim
@bioroboticseng.com
Department of Biosystems Engineering/Professor
Kangwon National University
RESEARCH, TEACHING, or OTHER INTERESTS
Multidisciplinary, Agricultural and Biological Sciences
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Sayan Deb Dutta, Jeong Man An, Jin Hexiu, Aayushi Randhawa, Keya Ganguly, Tejal V. Patil, Thavasyappan Thambi, Jangho Kim, Yong-kyu Lee, and Ki-Taek Lim
Elsevier BV
Aayushi Randhawa, Keya Ganguly, Sayan Deb Dutta, Tejal V. Patil, and Ki-Taek Lim
Elsevier BV
Tejal V. Patil, Hexiu Jin, Sayan Deb Dutta, Rumi Aacharya, Kehan Chen, Keya Ganguly, Aayushi Randhawa, and Ki-Taek Lim
Elsevier BV
Rumi Acharya, Tejal V. Patil, Sayan Deb Dutta, Jieun Lee, Keya Ganguly, Hojin Kim, Aayushi Randhawa, and Ki‐Taek Lim
Wiley
AbstractThe convergence of advanced nanotechnology with disease diagnosis has ushered in a transformative era in healthcare, empowering early and accurate detection of diseases and paving the way for timely interventions, improved treatment outcomes, and enhanced patient well‐being. The development of novel materials is frequently the impetus behind significant advancements in sensor technology. Among them, single‐walled carbon nanotubes (SWCNTSs) have emerged as promising nanomaterials for developing biosensors. Their unique optical, electrical, and biocompatibility properties make them promising candidates for enhancing the sensitivity and real‐time monitoring capabilities of biosensors, as well as for enabling various bioimaging techniques. Recent studies have demonstrated the utility of SWCNTS‐based biosensors in the real‐time monitoring of biological analytes, such as nitric oxide and hydrogen peroxide (H2O2), with potential implications for disease understanding and therapeutic response assessment. Moreover, SWCNTSs have shown promise in bioimaging applications, including fluorescence, Raman spectroscopy, and photoluminescence imaging of biological samples. This article delves into the core principles, design strategies, and operational mechanisms that underpin SWCNTS‐bioimaging techniques‐based biosensors. It emphasizes on their unique properties and versatile functionalization of carbon nanotubes, laying the foundation for their integration into biosensor platforms and applications aimed at diagnosing a wide spectrum of diseases including infectious diseases, cancer, neurological disorders, and metabolic conditions.
Md Moniruzzaman, Sayan Deb Dutta, Rumi Acharya, Ki-Taek Lim, and Jongsung Kim
Elsevier BV
Keya Ganguly, Rachmi Luthfikasari, Aayushi Randhawa, Sayan Deb Dutta, Tejal V. Patil, Rumi Acharya, and Ki‐Taek Lim
Wiley
AbstractMacrophages play an essential role in immunotherapy and tissue regeneration owing to their remarkable plasticity and diverse functions. Recent bioengineering developments have focused on using external physical stimuli such as electric and magnetic fields, temperature, and compressive stress, among others, on micro/nanostructures to induce macrophage polarization, thereby increasing their therapeutic potential. However, it is difficult to find a concise review of the interaction between physical stimuli, advanced micro/nanostructures, and macrophage polarization. This review examines the present research on physical stimuli‐induced macrophage polarization on micro/nanoplatforms, emphasizing the synergistic role of fabricated structure and stimulation for advanced immunotherapy and tissue regeneration. A concise overview of the research advancements investigating the impact of physical stimuli, including electric fields, magnetic fields, compressive forces, fluid shear stress, photothermal stimuli, and multiple stimulations on the polarization of macrophages within complex engineered structures, is provided. The prospective implications of these strategies in regenerative medicine and immunotherapeutic approaches are highlighted. This review will aid in creating stimuli‐responsive platforms for immunomodulation and tissue regeneration.
Youjin Seol, Keya Ganguly, Hojin Kim, Aayushi Randhawa, Tejal V. Patil, Sayan Deb Dutta, Rumi Acharya, and Ki-Taek Lim
Elsevier BV
Aayushi Randhawa, Sayan Deb Dutta, Keya Ganguly, Tejal V. Patil, and Ki‐Taek Lim
Wiley
Abstract3D printing and electrospinning are versatile techniques employed to produce 3D structures, such as scaffolds and ultrathin fibers, facilitating the creation of a cellular microenvironment in vitro. These two approaches operate on distinct working principles and utilize different polymeric materials to generate the desired structure. This review provides an extensive overview of these techniques and their potential roles in biomedical applications. Despite their potential role in fabricating complex structures, each technique has its own limitations. Electrospun fibers may have ambiguous geometry, while 3D‐printed constructs may exhibit poor resolution with limited mechanical complexity. Consequently, the integration of electrospinning and 3D‐printing methods may be explored to maximize the benefits and overcome the individual limitations of these techniques. This review highlights recent advancements in combined techniques for generating structures with controlled porosities on the micro–nano scale, leading to improved mechanical structural integrity. Collectively, these techniques also allow the fabrication of nature‐inspired structures, contributing to a paradigm shift in research and technology. Finally, the review concludes by examining the advantages, disadvantages, and future outlooks of existing technologies in addressing challenges and exploring potential opportunities.
Zhang Li, Hou Kegui, Wang Piao, Wang Xuejiu, Ki‐Taek Lim, and Hexiu Jin
Wiley
AbstractReconstruction of injured bone remains challenging in the clinic owing to the lack of suitable bone grafts. The utilization of PAI‐1 transfected‐conditioned media (P‐CM) has demonstrated its ability to facilitate the differentiation process of mesenchymal stem cells (MSCs), potentially serving as a crucial mediator in tissue regeneration. This research endeavored to explore the therapeutic potential of P‐CM concerning the differentiation of human bone marrow mesenchymal stem cells (hBMSCs). To assess new bone formation, a rat calvaria critical defect model was employed, while in vitro experiments involved the use of the alizarin Red‐S mineral induction test. In the rat calvaria critical defect model, P‐CM treatment resulted in significan new bone formation. In vitro, P‐CM treated hBMSCs displayed robust osteogenesis compared to the control group, as demonstrated by the mineral induction test using alizarin Red‐S. P‐CM with hydroxyapatite/β‐tricalcium phosphate/fibrin gel treatment significantly exhibited new bone formation, and the expression of osteogenic associated markers was enhanced in the P‐CM‐treated group. In conclusion, results demonstrate that P‐CM treatment significantly enhanced the osteogenic differantiation efficiency and new bone formation, thus could be used as an ideal therapeutic biomolecule for constructing bone‐specific implants, especially for orthopedic and dental applications.
Sayan Deb Dutta, Rachmi Luthfikasari, Tejal V. Patil, Keya Ganguly, Youjin Seol, Aayushi Randhawa, and Ki-Taek Lim
American Chemical Society (ACS)
Photosensitizing agents have received increased attention from the medical community, owing to their higher photothermal efficiency, induction of hyperthermia, and sustained delivery of bioactive molecules to their targets. Micro/nanorobots can be used as ideal photosensitizing agents by utilizing various physical stimuli for the targeted killing of pathogens (e.g., bacteria) and cancer cells. Herein, we report sunflower-pollen-inspired spiky zinc oxide (s-ZnO)-based nanorobots that effectively kill bacteria and cancer cells under near-infrared (NIR) light irradiation. The as-fabricated s-ZnO was modified with a catechol-containing photothermal agent, polydopamine (PDA), to improve its NIR-responsive properties, followed by the addition of antimicrobial (e.g., tetracycline/TCN) and anticancer (e.g., doxorubicin/DOX) drugs. The fabricated s-ZnO/PDA@Drug nanobots exhibited unique locomotory behavior with an average speed ranging from 13 to 14 μm/s under 2.0 W/cm2 NIR light irradiation. Moreover, the s-ZnO/PDA@TCN nanobots exhibited superior antibacterial activity against E. coli and S. epidermidis under NIR irradiation. The s-ZnO/PDA@DOX nanobots also displayed sufficient reactive oxygen species (ROS) amplification in B16F10 melanoma cells and induced apoptosis under NIR light, indicating their therapeutic efficacy. We hope the sunflower pollen-inspired s-ZnO nanorobots have tremendous potential in biomedical engineering from the phototherapy perspective, with the hope to reduce pathogen infections.
Hyeonseo Park, Tejal V. Patil, Sayan Deb Dutta, Jieun Lee, Keya Ganguly, Aayushi Randhawa, Hojin Kim, and Ki‐Taek Lim
Wiley
AbstractThe skin serves as the body's outermost barrier and is the largest organ, providing protection not only to the body but also to various internal organs. Owing to continuous exposure to various external factors, it is susceptible to damage that can range from simple to severe, including serious types of wounds such as burns or chronic wounds. Macrophages play a crucial role in the entire wound healing process and contribute significantly to skin regeneration. Initially, M1 macrophages infiltrate to phagocytose bacteria, debris, and dead cells in fresh wounds. As tissue repair is activated, M2 macrophages are promoted, reducing inflammation and facilitating restoration of the dermis and epidermis to regenerate the tissue. Based on this understanding, developing extracellular matrix (ECM) mimicking structures is suitable for promoting cell attachment, proliferation, migration, and macrophage polarization. Among the numerous strategies, electrospinning is a versatile technique for obtaining ECM‐mimicking structures with anisotropic and isotropic topologies of micro/nanofibers. The use of various biomaterials in the fabrication of nanofibers with anisotropic and isotropic topologies can influence macrophage polarization. Moreover, these fibers possess a high surface‐area‐to‐volume ratio, promoting the effective exchange of vital nutrients and oxygen, which are crucial for cell viability and tissue regeneration. Micro/nanofibers with diverse physical and chemical properties can be tailored to polarize macrophages toward skin regeneration and wound healing, depending on specific requirements. This review describes the significance of micro/nano structures for activating macrophages and promoting wound healing.This article is protected by copyright. All rights reserved
Dinesh K. Patel, So-Yeon Won, Eunseo Jung, Sayan Deb Dutta, Tejal V. Patil, Ki-Taek Lim, and Sung Soo Han
Elsevier BV
Dinesh K. Patel, So-Yeon Won, Tejal V. Patil, Sayan Deb Dutta, Ki-Taek Lim, and Sung Soo Han
Elsevier BV
Hojin Kim, Sayan Deb Dutta, Aayushi Randhawa, Tejal V. Patil, Keya Ganguly, Rumi Acharya, Jieun Lee, Hyeonseo Park, and Ki-Taek Lim
Elsevier BV
Sayan Deb Dutta, Keya Ganguly, Aayushi Randhawa, Tejal V. Patil, Hojin Kim, Rumi Acharya, and Ki‐Taek Lim
Wiley
AbstractIn recent years, additive manufacturing tools, such as 3D printing, has gained enormous attention in biomedical engineering for developing ionotropic devices, flexible electronics, skin‐electronic interfaces, and wearable sensors with extremely high precision and sensing accuracy. Such printed bioelectronics are innovative and can be used as multi‐stimuli response platforms for human health monitoring and disease diagnosis. This review systematically discusses the past, present, and future of the various printable and stretchable soft bioelectronics for precision medicine. The potential of various naturally and chemically derived conductive biopolymer inks and their nanocomposites with tunable physico‐chemical properties is also highlighted, which is crucial for bioelectronics fabrication. Then, the design strategies of various printable sensors for human body sensing are summarized. In conclusion, the perspectives on the future advanced bioelectronics are described, which will be helpful, particularly in the field of nano/biomedicine. An in‐depth knowledge of materials design to functional aspects of printable bioelectronics is demonstrated, with an aim to accelerate the development of next‐generation wearables.
Jieun Lee, Sayan Deb Dutta, Rumi Acharya, Hyeonseo Park, Hojin Kim, Aayushi Randhawa, Tejal V. Patil, Keya Ganguly, Rachmi Luthfikasari, and Ki‐Taek Lim
Wiley
AbstractConductive hydrogels (CHs) are promising alternatives for electrical stimulation of cells and tissues in biomedical engineering. Wound healing and immunomodulation are complex processes that involve multiple cell types and signaling pathways. 3D printable conductive hydrogels have emerged as an innovative approach to promote wound healing and modulate immune responses. CHs can facilitate electrical and mechanical stimuli, which can be beneficial for altering cellular metabolism and enhancing the efficiency of the delivery of therapeutic molecules. This review summarizes the recent advances in 3D printable conductive hydrogels for wound healing and their effect on macrophage polarization. This report also discusses the properties of various conductive materials that can be used to fabricate hydrogels to stimulate immune responses. Furthermore, this review highlights the challenges and limitations of using 3D printable CHs for future material discovery. Overall, 3D printable conductive hydrogels hold excellent potential for accelerating wound healing and immune responses, which can lead to the development of new therapeutic strategies for skin and immune‐related diseases.
Kyoung-Je Jang, Sangbae Park, Juo Lee, Yeonggeol Hong, Hoon Seonwoo, Ki-Taek Lim, Jangho Kim, and Jong Hoon Chung
Springer Science and Business Media LLC
Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, Rumi Acharya, Md Moniruzzaman, and Ki-Taek Lim
Elsevier BV
Rumi Acharya, Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, and Ki-Taek Lim
MDPI AG
Electroactive polymer–metal composites (EAPMCs) have gained significant attention in tissue engineering owing to their exceptional mechanical and electrical properties. EAPMCs develop by combining an electroactive polymer matrix and a conductive metal. The design considerations include choosing an appropriate metal that provides mechanical strength and electrical conductivity and selecting an electroactive polymer that displays biocompatibility and electrical responsiveness. Interface engineering and surface modification techniques are also crucial for enhancing the adhesion and biocompatibility of composites. The potential of EAPMC-based tissue engineering revolves around its ability to promote cellular responses, such as cell adhesion, proliferation, and differentiation, through electrical stimulation. The electrical properties of these composites can be used to mimic natural electrical signals within tissues and organs, thereby aiding tissue regeneration. Furthermore, the mechanical characteristics of the metallic components provide structural reinforcement and can be modified to align with the distinct demands of various tissues. EAPMCs have extraordinary potential as regenerative biomaterials owing to their ability to promote beneficial effects in numerous electrically responsive cells. This study emphasizes the characteristics and applications of EAPMCs in tissue engineering.
Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, and Ki-Taek Lim
Elsevier BV
Dinesh K. Patel, Tejal V. Patil, Keya Ganguly, Sayan Deb Dutta, and Ki-Taek Lim
Elsevier BV
Sayan Deb Dutta, Keya Ganguly, Jin Hexiu, Aayushi Randhawa, Md Moniruzzaman, and Ki‐Taek Lim
Wiley
One of the significant challenges in bone tissue engineering (BTE) is the healing of traumatic tissue defects which requires longer time to recover owing to the recruitment of local infection and delayed angiogenesis. Various strategies, such as hydrogel dressings, growth factors delivery, and stem cell therapy has been shown potential alternative to the traumatic tissue repair; however, limited their actual clinical application due to the socio-economic burden. Herein, we reported a 3D printable multi-functional hydrogel scaffold composing polyphenolic carbon quantum dots (CQDs, 100 ug mL-1 ) and gelatin methacryloyl (GelMA, 12 wt.%) for bone regeneration and anti-tumor therapy. The CQDs was synthesized from a plant-inspired bioactive molecule, 1, 3, 5-trihydroxybenzene (a polyphenol) via facile wet chemistry method. The 3D printed GelMA-CQDs hydrogels displayed typical shear-thinning behavior with excellent printability. Our results demonstrated that the nanocomposite 3D hydrogel promoted M2 polarization of macrophage (Raw 264.7) cells via upregulation of anti-inflammatory genes (e.g., IL-4 and IL10), and induced angiogenesis and osteogenesis of human bone mesenchymal stem cells (hBMSCs). The bioprinted hBMSCs were able to produced vessel-like structures in the presence of GelMA-CQDs hydrogel after 14 days of incubation. Furthermore, the 3D printed scaffolds also showed remarkable near infra-red (NIR) responsive properties under 808 nm NIR light (1.0 W cm-2 ) irradiation and showed controlled release of antitumor drugs (∼49%) at pH 6.5, and thereby killing the osteosarcoma cells. Therefore, we anticipate that the tissue regeneration and healing ability with therapeutic potential of the 3D printed GelMA-CQDs scaffolds may provide a promising alternative for traumatic tissue regeneration via augmenting angiogenesis and accelerated immunomodulation. This article is protected by copyright. All rights reserved.
Keya Ganguly, Sayan Deb Dutta, Aayushi Randhawa, Dinesh K. Patel, Tejal V. Patil, and Ki‐Taek Lim
Wiley
Biomimetic soft hydrogels used in bone tissue engineering frequently produce unsatisfactory outcomes. Here, it is investigated how human bone‐marrow‐derived mesenchymal stem cells (hBMSCs) differentiated into early osteoblasts on remarkably soft 3D hydrogel (70 ± 0.00049 Pa). Specifically, hBMSCs seeded onto cellulose nanocrystals incorporated methacrylate gelatin hydrogels are subjected to pulsatile pressure stimulation (PPS) of 5–20 kPa for 7 days. The PPS stimulates cellular processes such as mechanotransduction, cytoskeletal distribution, prohibition of oxidative stress, calcium homeostasis, osteogenic marker gene expression, and osteo‐specific cytokine secretions in hBMSCs on soft substrates. The involvement of Piezo 1 is the main ion channel involved in mechanotransduction. Additionally, RNA‐sequencing results reveal differential gene expression concerning osteogenic differentiation, bone mineralization, ion channel activity, and focal adhesion. These findings suggest a practical and highly scalable method for promoting stem cell commitment to osteogenesis on soft matrices for clinical reconstruction.
Sayan Deb Dutta, Keya Ganguly, Aayushi Randhawa, Tejal V. Patil, Dinesh K. Patel, and Ki-Taek Lim
Elsevier BV
Tejal V. Patil, Sayan Deb Dutta, Dinesh K. Patel, Keya Ganguly, and Ki-Taek Lim
Elsevier BV