Ki-Taek Lim
@bioroboticseng.com
Department of Biosystems Engineering/Professor
Kangwon National University
RESEARCH, TEACHING, or OTHER INTERESTS
Multidisciplinary, Agricultural and Biological Sciences
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Dinesh K. Patel, So-Yeon Won, Eunseo Jung, Sayan Deb Dutta, Tejal V. Patil, Ki-Taek Lim, and Sung Soo Han
Elsevier BV
Dinesh K. Patel, So-Yeon Won, Tejal V. Patil, Sayan Deb Dutta, Ki-Taek Lim, and Sung Soo Han
Elsevier BV
Hojin Kim, Sayan Deb Dutta, Aayushi Randhawa, Tejal V. Patil, Keya Ganguly, Rumi Acharya, Jieun Lee, Hyeonseo Park, and Ki-Taek Lim
Elsevier BV
Sayan Deb Dutta, Keya Ganguly, Aayushi Randhawa, Tejal V. Patil, Hojin Kim, Rumi Acharya, and Ki‐Taek Lim
Wiley
AbstractIn recent years, additive manufacturing tools, such as 3D printing, has gained enormous attention in biomedical engineering for developing ionotropic devices, flexible electronics, skin‐electronic interfaces, and wearable sensors with extremely high precision and sensing accuracy. Such printed bioelectronics are innovative and can be used as multi‐stimuli response platforms for human health monitoring and disease diagnosis. This review systematically discusses the past, present, and future of the various printable and stretchable soft bioelectronics for precision medicine. The potential of various naturally and chemically derived conductive biopolymer inks and their nanocomposites with tunable physico‐chemical properties is also highlighted, which is crucial for bioelectronics fabrication. Then, the design strategies of various printable sensors for human body sensing are summarized. In conclusion, the perspectives on the future advanced bioelectronics are described, which will be helpful, particularly in the field of nano/biomedicine. An in‐depth knowledge of materials design to functional aspects of printable bioelectronics is demonstrated, with an aim to accelerate the development of next‐generation wearables.
Jieun Lee, Sayan Deb Dutta, Rumi Acharya, Hyeonseo Park, Hojin Kim, Aayushi Randhawa, Tejal V. Patil, Keya Ganguly, Rachmi Luthfikasari, and Ki‐Taek Lim
Wiley
AbstractConductive hydrogels (CHs) are promising alternatives for electrical stimulation of cells and tissues in biomedical engineering. Wound healing and immunomodulation are complex processes that involve multiple cell types and signaling pathways. 3D printable conductive hydrogels have emerged as an innovative approach to promote wound healing and modulate immune responses. CHs can facilitate electrical and mechanical stimuli, which can be beneficial for altering cellular metabolism and enhancing the efficiency of the delivery of therapeutic molecules. This review summarizes the recent advances in 3D printable conductive hydrogels for wound healing and their effect on macrophage polarization. This report also discusses the properties of various conductive materials that can be used to fabricate hydrogels to stimulate immune responses. Furthermore, this review highlights the challenges and limitations of using 3D printable CHs for future material discovery. Overall, 3D printable conductive hydrogels hold excellent potential for accelerating wound healing and immune responses, which can lead to the development of new therapeutic strategies for skin and immune‐related diseases.
Hyeonseo Park, Tejal V. Patil, Sayan Deb Dutta, Jieun Lee, Keya Ganguly, Aayushi Randhawa, Hojin Kim, and Ki‐Taek Lim
Wiley
AbstractThe skin serves as the body's outermost barrier and is the largest organ, providing protection not only to the body but also to various internal organs. Owing to continuous exposure to various external factors, it is susceptible to damage that can range from simple to severe, including serious types of wounds such as burns or chronic wounds. Macrophages play a crucial role in the entire wound healing process and contribute significantly to skin regeneration. Initially, M1 macrophages infiltrate to phagocytose bacteria, debris, and dead cells in fresh wounds. As tissue repair is activated, M2 macrophages are promoted, reducing inflammation and facilitating restoration of the dermis and epidermis to regenerate the tissue. Based on this understanding, developing extracellular matrix (ECM) mimicking structures is suitable for promoting cell attachment, proliferation, migration, and macrophage polarization. Among the numerous strategies, electrospinning is a versatile technique for obtaining ECM‐mimicking structures with anisotropic and isotropic topologies of micro/nanofibers. The use of various biomaterials in the fabrication of nanofibers with anisotropic and isotropic topologies can influence macrophage polarization. Moreover, these fibers possess a high surface‐area‐to‐volume ratio, promoting the effective exchange of vital nutrients and oxygen, which are crucial for cell viability and tissue regeneration. Micro/nanofibers with diverse physical and chemical properties can be tailored to polarize macrophages toward skin regeneration and wound healing, depending on specific requirements. This review describes the significance of micro/nano structures for activating macrophages and promoting wound healing.This article is protected by copyright. All rights reserved
Aayushi Randhawa, Sayan Deb Dutta, Keya Ganguly, Tejal V. Patil, and Ki‐Taek Lim
Wiley
Abstract3D printing and electrospinning are versatile techniques employed to produce 3D structures, such as scaffolds and ultrathin fibers, facilitating the creation of a cellular microenvironment in vitro. These two approaches operate on distinct working principles and utilize different polymeric materials to generate the desired structure. This review provides an extensive overview of these techniques and their potential roles in biomedical applications. Despite their potential role in fabricating complex structures, each technique has its own limitations. Electrospun fibers may have ambiguous geometry, while 3D‐printed constructs may exhibit poor resolution with limited mechanical complexity. Consequently, the integration of electrospinning and 3D‐printing methods may be explored to maximize the benefits and overcome the individual limitations of these techniques. This review highlights recent advancements in combined techniques for generating structures with controlled porosities on the micro–nano scale, leading to improved mechanical structural integrity. Collectively, these techniques also allow the fabrication of nature‐inspired structures, contributing to a paradigm shift in research and technology. Finally, the review concludes by examining the advantages, disadvantages, and future outlooks of existing technologies in addressing challenges and exploring potential opportunities.
Kyoung-Je Jang, Sangbae Park, Juo Lee, Yeonggeol Hong, Hoon Seonwoo, Ki-Taek Lim, Jangho Kim, and Jong Hoon Chung
Springer Science and Business Media LLC
Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, Rumi Acharya, Md Moniruzzaman, and Ki-Taek Lim
Elsevier BV
Rumi Acharya, Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, and Ki-Taek Lim
MDPI AG
Electroactive polymer–metal composites (EAPMCs) have gained significant attention in tissue engineering owing to their exceptional mechanical and electrical properties. EAPMCs develop by combining an electroactive polymer matrix and a conductive metal. The design considerations include choosing an appropriate metal that provides mechanical strength and electrical conductivity and selecting an electroactive polymer that displays biocompatibility and electrical responsiveness. Interface engineering and surface modification techniques are also crucial for enhancing the adhesion and biocompatibility of composites. The potential of EAPMC-based tissue engineering revolves around its ability to promote cellular responses, such as cell adhesion, proliferation, and differentiation, through electrical stimulation. The electrical properties of these composites can be used to mimic natural electrical signals within tissues and organs, thereby aiding tissue regeneration. Furthermore, the mechanical characteristics of the metallic components provide structural reinforcement and can be modified to align with the distinct demands of various tissues. EAPMCs have extraordinary potential as regenerative biomaterials owing to their ability to promote beneficial effects in numerous electrically responsive cells. This study emphasizes the characteristics and applications of EAPMCs in tissue engineering.
Sayan Deb Dutta, Tejal V. Patil, Keya Ganguly, Aayushi Randhawa, and Ki-Taek Lim
Elsevier BV
Dinesh K. Patel, Tejal V. Patil, Keya Ganguly, Sayan Deb Dutta, and Ki-Taek Lim
Elsevier BV
Sayan Deb Dutta, Keya Ganguly, Jin Hexiu, Aayushi Randhawa, Md Moniruzzaman, and Ki‐Taek Lim
Wiley
One of the significant challenges in bone tissue engineering (BTE) is the healing of traumatic tissue defects which requires longer time to recover owing to the recruitment of local infection and delayed angiogenesis. Various strategies, such as hydrogel dressings, growth factors delivery, and stem cell therapy has been shown potential alternative to the traumatic tissue repair; however, limited their actual clinical application due to the socio-economic burden. Herein, we reported a 3D printable multi-functional hydrogel scaffold composing polyphenolic carbon quantum dots (CQDs, 100 ug mL-1 ) and gelatin methacryloyl (GelMA, 12 wt.%) for bone regeneration and anti-tumor therapy. The CQDs was synthesized from a plant-inspired bioactive molecule, 1, 3, 5-trihydroxybenzene (a polyphenol) via facile wet chemistry method. The 3D printed GelMA-CQDs hydrogels displayed typical shear-thinning behavior with excellent printability. Our results demonstrated that the nanocomposite 3D hydrogel promoted M2 polarization of macrophage (Raw 264.7) cells via upregulation of anti-inflammatory genes (e.g., IL-4 and IL10), and induced angiogenesis and osteogenesis of human bone mesenchymal stem cells (hBMSCs). The bioprinted hBMSCs were able to produced vessel-like structures in the presence of GelMA-CQDs hydrogel after 14 days of incubation. Furthermore, the 3D printed scaffolds also showed remarkable near infra-red (NIR) responsive properties under 808 nm NIR light (1.0 W cm-2 ) irradiation and showed controlled release of antitumor drugs (∼49%) at pH 6.5, and thereby killing the osteosarcoma cells. Therefore, we anticipate that the tissue regeneration and healing ability with therapeutic potential of the 3D printed GelMA-CQDs scaffolds may provide a promising alternative for traumatic tissue regeneration via augmenting angiogenesis and accelerated immunomodulation. This article is protected by copyright. All rights reserved.
Keya Ganguly, Sayan Deb Dutta, Aayushi Randhawa, Dinesh K. Patel, Tejal V. Patil, and Ki‐Taek Lim
Wiley
Biomimetic soft hydrogels used in bone tissue engineering frequently produce unsatisfactory outcomes. Here, it is investigated how human bone‐marrow‐derived mesenchymal stem cells (hBMSCs) differentiated into early osteoblasts on remarkably soft 3D hydrogel (70 ± 0.00049 Pa). Specifically, hBMSCs seeded onto cellulose nanocrystals incorporated methacrylate gelatin hydrogels are subjected to pulsatile pressure stimulation (PPS) of 5–20 kPa for 7 days. The PPS stimulates cellular processes such as mechanotransduction, cytoskeletal distribution, prohibition of oxidative stress, calcium homeostasis, osteogenic marker gene expression, and osteo‐specific cytokine secretions in hBMSCs on soft substrates. The involvement of Piezo 1 is the main ion channel involved in mechanotransduction. Additionally, RNA‐sequencing results reveal differential gene expression concerning osteogenic differentiation, bone mineralization, ion channel activity, and focal adhesion. These findings suggest a practical and highly scalable method for promoting stem cell commitment to osteogenesis on soft matrices for clinical reconstruction.
Sayan Deb Dutta, Keya Ganguly, Aayushi Randhawa, Tejal V. Patil, Dinesh K. Patel, and Ki-Taek Lim
Elsevier BV
Tejal V. Patil, Sayan Deb Dutta, Dinesh K. Patel, Keya Ganguly, and Ki-Taek Lim
Elsevier BV
Dinesh K. Patel, Keya Ganguly, Sayan Deb Dutta, Tejal V. Patil, and Ki-Taek Lim
Elsevier BV
Dinesh K. Patel, Keya Ganguly, Sayan Deb Dutta, Tejal V. Patil, Aayushi Randhawa, and Ki-Taek Lim
Elsevier BV
Sayan Deb Dutta, Md Moniruzzaman, Jin Hexiu, Sourav Sarkar, Keya Ganguly, Dinesh K. Patel, Jagannath Mondal, Yong-Kyu Lee, Rumi Acharya, Jongsung Kim,et al.
American Chemical Society (ACS)
Recent studies indicate that mitochondrial dysfunctions and DNA damage have a critical influence on cell survival, which is considered one of the therapeutic targets for cancer therapy. In this study, we demonstrated a comparative study of the effect of polyphenolic carbon quantum dots (CQDs) on in vitro and in vivo antitumor efficacy. Dual emissive (green and yellow) shape specific polyphenolic CQDs (G-CQDs and Y-CQDs) were synthesized from easily available nontoxic precursors (phloroglucinol), and the antitumor property of the as-synthesized probe was investigated as compared to round-shaped blue emissive CQDs (B-CQDs) derived from well-reported precursor citric acid and urea. The B-CQDs had a nuclei-targeting property, and G-CQDs and Y-CQDs had mitochondria-targeting properties. We have found that the polyphenol containing CQDs (at a dose of 100 μg mL-1) specifically attack mitochondria by excess accumulation, altering the metabolism, inhibiting branching pattern, imbalanced Bax/Bcl-2 homeostasis, and ultimately generating oxidative stress levels, leading to oxidative stress-induced cell death in cancer cells in vitro. We show that G-CQDs are the main cause of oxidative stress in cancer cells because of their ability to produce sufficient •OH- and 1O2 radicals, evidenced by electron paramagnetic resonance spectroscopy and a terephthalic acid test. Moreover, the near-infrared absorption properties of the CQDs were exhibited in two-photon (TP) emission, which was utilized for TP cellular imaging of cancer cells without photobleaching. The in vivo antitumor test further discloses that intratumoral injection of G-CQDs can significantly augment the treatment efficacy of subcutaneous tumors without any adverse effects on BalB/c nude mice. We believe that shape-specific polyphenolic CQD-based nanotheranostic agents have a potential role in tumor therapy, thus proving an insight on treatment of malignant cancers.
AAYUSHI RANDHAWA, SAYAN DEB DUTTA, KEYA GANGULY, TEJAL V. PATIL, RACHMI LUTHFIKASARI, and KI-TAEK LIM
Computers, Materials and Continua (Tech Science Press)
Aayushi Randhawa, Sayan Deb Dutta, Keya Ganguly, Dinesh K. Patel, Tejal V. Patil, and Ki‐Taek Lim
Wiley
The conversion of liquid resin into solid structures upon exposure to light of a specific wavelength is known as photopolymerization or photo-curable 3D printing. In recent years, photopolymerization-based 3D printing has gained enormous attention in tissue engineering for constructing highly complex and precise tissue structures. Due to the economic and environmental benefits of the biopolymers employed, photo-curable 3D printing is considered highly accurate and an alternative method for replacing damaged tissues. However, the lack of suitable bio-based photopolymers, their characterization, effective crosslinking strategies, and optimal printing conditions are hindering the extensive application and commercialization of those materials in the global market. This review highlights the present status of various photopolymers, their synthesis techniques, and optimization parameters for biomedical applications. Moreover, we also discussed a glimpse of various photopolymerization techniques that are currently employed for 3D printing. Furthurmore, we also reviewed the influence of various naturally-derived nanomaterial reinforced polymerization and their influence on printability and shape fidelity. Finally, the ultimate use of those photopolymerized hydrogel scaffolds in tissue engineering is also discussed. Taken together, we believe that photopolymerized 3D printing has a great future, whereas conventional 3D printing requires considerable sophistication, and this review will provide the readers with a comprehensive approach to the development of light-mediated 3D printing for tissue engineering applications. This article is protected by copyright. All rights reserved.
Md Moniruzzaman, Sayan Deb Dutta, Ki-Taek Lim, and Jongsung Kim
American Chemical Society (ACS)
Little progress has been achieved on the synthesis of hydrophilic carbon dots (CDs), derived from polycyclic aromatic hydrocarbons, as an excellent photothermal agent. In this study, a strategy was developed to synthesize highly photoluminescent greenish-yellow emissive CDs based on nitration followed by hydrothermal carbonization of the polycyclic aromatic hydrocarbon precursor, perylene. The perylene-derived CDs (PY-CDs) exhibited an excellent NIR-light (808 nm) harvesting property toward high photothermal conversion efficiency (PCE = ∼56.7%) and thus demonstrated remarkable NIR-light responsive photothermal bactericidal performance. Furthermore, these fluorescent PY-CD nanoprobes displayed excitation-dependent polychromatic emissions in the range of 538–600 nm, with the maximum emission at 538 nm. This enables intense multicolor biological imaging of cellular substances with long-term photostability, nontoxicity, and effective subcellular distribution. The bactericidal action of PY-CDs is likely due to the elevated reactive oxygen species amplification in cooperation with the hyperthermia effect. This study offers a potential substitute for multicolor imaging-guided metal-free carbon-based photothermal therapy.
Md Moniruzzaman, Sayan Deb Dutta, Ki-Taek Lim, and Jongsung Kim
Elsevier BV
Sayan Deb Dutta, Keya Ganguly, Min-Soo Jeong, Dinesh K. Patel, Tejal V. Patil, Seong-Jun Cho, and Ki-Taek Lim
American Chemical Society (ACS)
Lab-grown bovine meat analogues are emerging alternatives to animal sacrifices for cultured meat production. The most challenging aspect of the production process is the rapid proliferation of cells and establishment of the desired 3D structure for mass production. In this study, we developed a direct ink writing-based 3D-bioprinted meat culture platform composed of 6% (w/v) alginate and 4% (w/v) gelatin (Alg/Gel)-based hydrogel scaffolds supplemented with naturally derived protein hydrolysates (PHs; 10%) from highly nutritive plants (soybean, pigeon pea, and wheat), and some selected edible insects (beetles, crickets, and mealworms) on in vitro proliferation of bovine myosatellite cells (bMSCs) extracted from fresh meat samples. The developed bioink exhibited excellent shear-thinning behavior (n < 1) and mechanical stability during 3D bioprinting. Commercial proteases (Alcalase, Neutrase, and Flavourzyme) were used for protein hydrolysis. The resulting hydrolysates exhibited lower-molecular-weight bands (12-50 kDa) than those of crude isolates (55-160 kDa), as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The degree of hydrolysis was higher in the presence of Alcalase for both plant (34%) and insect (62%) PHs than other enzymes. The 3D-printed hydrogel scaffolds displayed excellent bioactivity and stability after 7 days of incubation. The developed prototype structure (pepperoni meat, 20 × 20 × 5 mm) provided a highly stable, nutritious, and mechanically strong structure that supported the rapid proliferation of myoblasts in a low-serum environment during the entire culture period. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay enhanced the free radical reduction of Alcalase- and Neutrase-treated PHs. Furthermore, the bioprinted bMSCs displayed early myogenesis (desmin and Pax7) in the presence of PHs, suggesting its role in bMSC differentiation. In conclusion, we developed a 3D bioprinted and bioactive meat culture platform using Alg/Gel/PHs as a printable and edible component for the mass production of cultured meat.
Dinesh K. Patel, Keya Ganguly, Sayan Deb Dutta, Tejal V. Patil, and Ki-Taek Lim
Elsevier BV