Larisa Gunina-Orlova

@dpu.edu.ua

Professor of Department of technologies of health improvement and physical culture and sports rehabilitation
State Tax University

Larisa Gunina-Orlova


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https://researchid.co/larisagunina

EDUCATION

biologist-biochemist; physician-therapist

RESEARCH, TEACHING, or OTHER INTERESTS

Clinical Biochemistry, Physiology, Pharmacology (medical), Pharmacology
31

Scopus Publications

Scopus Publications

  • Creatine as an essential pharmaconutrient in the practice of training athletes in martial arts
    I. B. Samura, L. M. Gunina-Orlova, O. I. Orlov, A. M. Hurieieva, O. Ye. Chernenko, et al.
    Modern Medical Technology, 2026
    Aim: to form modern ideas about the properties of creatine as a pharmaconutrient, its biological role in the body, and rational dosage during physical exertion. Materials and methods. To determine the level of development of the research problem, a search was conducted in the databases MEDLINE, PubMed, Scopus, Directory of Open Access Journals and ScienceDirect; the keywords used included physical exercise, creatine supplementation, oxidative stress, mitochondria, energy metabolism. We focused on English-language articles published between 2019 and 2025. Results. The use of creatine loading schemes during high-intensity interval training provides an increase in physical fitness indicators by an average of 10–20 % due to an increase in the concentration of muscle phosphocreatine. According to modern ideas, creatine belongs, on the one hand, to the group of myostatin inhibitors, and on the other hand, to the protectors of mitochondrial function. The results of 50 studies showed that compared to placebo, creatine, both alone and in combination with bicarbonate, produced a statistically significant increase in average and peak power in martial artists. Increasing cellular creatine levels promoted metabolic channeling, demonstrated antiapoptotic properties, promoted the survival of dopaminergic neurons, and produced a pronounced antidepressant effect, helping athletes remain psychologically stable during training, and before competitions. Conclusions. Thus, from the literature data, it becomes clear that the course use of creatine leads to a significant increase in the efficiency of the training process, and this makes it an indispensable tool for improving the indicators of physical and functional fitness, as well as the overall quality of life of athletes. Creatine is a universal ergogenic food supplement, but due to its predominantly positive effect on the body with chiefly aerobic energy supply, typical of Olympic martial arts, it is fully suitable for improving the effectiveness of the training process in boxing, judo, taekwondo, jiu-jitsu (jujutsu), fencing, etc. The neuroprotective and antidepressant effects of ergogenic supplements with creatine are very important in sports such as martial arts, as they contribute to the development of discipline, emotional control, mental stability, and help athletes’ brains become more resistant to stress, especially before competitions. The course use of such a pharmaconutrient as creatine can rightfully be attributed to health-saving technologies – a new direction in sports pharmacology, and nutriology.
  • The role of lactate in the body of martial artists during physical activity: a new look at the problem
    I. F. Bielenichev, L. M. Gunina, O. I. Orlov, I. B. Samura, E. Yu. Doroshenko, et al.
    Modern Medical Technology, 2025
    Aim. To conduct a modern semantic analysis of the results of our own research and literature data on the physiological significance of increased lactate level in skeletal muscles during physical activity in martial artists. Materials and methods. This study used bibliosemantic, analytical, logical, and generalization methods. The bibliographic databases of life sciences and biomedical information MEDLINE, EMBASE, Medline (PubMed), Web of Science, and Cochrane Central were searched to find publications in English that matched the research keywords. The authors carried out an independent search and selection of articles, assessment of data quality, compliance of the presentation and interpretation with the main idea of the study, with the formation of the final list of references. Results. For many years, the role of lactate in the formation of physical fatigue and delayed muscle soreness in martial artists and other athletes remained unknown. There has been a lot of speculation and competing concepts. Over the past 20 years, due to the successes of modern biochemistry, physiology, and molecular pharmacology, subtle molecular-biochemical mechanisms of delayed muscle soreness formation after muscle loading have been revealed. However, a holistic attitude to the role of lactate has not been formulated. We analyzed the results of modern studies, which showed that oxidative stress, inflammation, and a decrease in cytoprotective proteins, in particular HSP70, but not the accumulation of lactate, are more important in the formation of delayed onset muscle soreness (DOMS). Conclusions. Accumulation of lactate in muscle cells with its subsequent release into the bloodstream is considered to be much more positive than negative factor, and therefore the views on the increase of lactate content and its assessment as an enemy of athletes, including martial artists, should be reconsidered.
  • EVALUATION OF THE EFFICIENCY OF THE NEUROPROTECTIVE DRUGS AFTER PRENATAL HYPOXIA
    I.F. Belenichev, O.G. Aliyeva, L.M. Gunina, N.V. Bukhtiyarova, and
    Fiziologichnyi Zhurnal, 2023
    We studied the effect of angiolin, thiotriazoline, tamoxifen, glutaredoxin, cerebrocurin, mildronate, nicomex, L-arginine, HSF-1, and the reference drug piracetam on molecular markers of neurodestruction/neuroprotection in a model of chronic hemic prenatal hypoxia (PH) for experimentally substantiate the prospects for further study of these drugs as components of complex treatment of central nervous system damage at prenatal hypoxic. The concentration of HSP70, metalloproteinase-8 (MPP-8), and nitrotyrosine in the blood plasma of rats on days 30 and 60 after PH was studied by enzyme immunoassay. It has been established that chronic PH leads to an increase in the concentration of nitrotyrosine, MMP8, and inhibition of the synthesis of HSP70, which indicates a violation of the mechanisms of neuroprotection/neurodestruction processes regulation. Course injections of the studied preparations led to an increase in the level of HSP70 in the blood serum of animals and a decrease in the concentration of nitrotyrosine and MPP-8 with a prolonged effect. Cerebrocurin (150 mg/ kg), Angiolin (50 mg/kg), HSF-1 (50 mg/kg) and Glutaredoxin (200 μg/kg) most actively affected the parameters of the studied molecular markers, so they can be considered as promising neuroprotective agents means in complex therapy after PH.
  • DETERMINATION OF THE INFLUENCE MECHANISMS OF SUCCINIC ACID-BASED DRUG ON IMPROVING THE STATE OF ERYTHROCYTE LINK OF OXYGEN TRANSPORT DURING AEROBIC PHYSICAL LOADS.
    Larisa Gunina, Yurii Ataman, Ihor Belenichev, Roman Golovashchenko, Valentina Voitenko, et al.
    Eastern Ukrainian Medical Journal, 2022
    Introduction. Strenuous aerobic work inherent to cyclical sports requires adequate oxygenation of the athletes’ working muscles. One of the ways to improve the oxygen transport function of blood is to optimize the structural and functional state of erythrocytes, for example, by using succinic acid in the form of the medical drug Armadin Long. This pharmacological drug is widespread in clinical practice for treating hypoxic and ischemic conditions, and very little is known about its use for improving the condition of the erythrocytes in the blood of athletes, which determined the relevance of this study. The objective of the study was to assess the feasibility and effectiveness of using the medical drug Armadine Long to improve the state of the erythrocyte link of the blood oxygen transport system during aerobic exercise. Materials and Methods. A randomized blinded placebo-controlled trial included 40 male middle-distance runners (aerobic discipline of athletics). The subjects were divided into two subgroups matched for number, age, and anthropometric characteristics (strats). In this work, laboratory methods were used, including hematological tests (in particular, hemoglobin and erythrocytes level measurement and erythrocyte characteristics evaluation), as well as biochemical method: the study of prooxidant and antioxidant balance (activity of lipid peroxidation according to changes in the content of maloniс dialdehyde and antioxidant protection according to changes in the concentration of reduced glutathione) and functional characteristics of erythrocyte membranes (permeability, sorption capacity, sorption capacity of the glycocalyx) as well as evaluation of the main components of lipid and protein composition of red blood cell membranes. Pedagogical research methods were based on the determination of relative aerobic capacity using the generally accepted PWC170 test. Results of the study. During 21 days of intensive loads, an almost two-fold increase in contents of malonic dialdehyde was observed with a parallel decrease in reduced glutathione content by 23.5 % during aerobic loads. At the same time, there was a deterioration of the functional characteristics of erythrocytes and a decrease in the relative aerobic capacity in comparison with the baseline data. The use of the medical drug Armadine Long at a dose of 600 mg per day improved the indicated characteristics of erythrocytes. At the same time, there were positive changes in the protein and lipid state of the erythrocyte membranes, and the athletes’ aerobic power increased by 38.7%. It substantiates the high ergogenic ability of succinic acid, which is based on the normalization of the lipoperoxidation process and the improvement of the structural and functional characteristics of erythrocyte membranes.
  • ENERGY SUPPLY OF THE HEART AND SKELETAL MUSCLES DURING EXERCISE: MITOCHONDRIAL VECTOR (REVIEW)
    L.M. Gunina, I.F. Belenichev, K.V. Rosova, Yu.O. Ataman, V.L. Voitenko, et al.
    Fiziologichnyi Zhurnal, 2022
    In this review, we consider metabolic pathways of energy production from the standpoint of determining the mechanisms of energy supply during intense physical activity and the need to accelerate the process of ATP resynthesis; possible directions of this process intensification are outlined. The most important systems for an athlete to limit his physical performance are the cardiovascular and muscular. That is why we emphasize on energy production in myocardial cells and myocytes. Energy production and energy exchange in cells is carried out by mitochondria, which are the main organelles of energy supply. Functional activity of these organelles is provided by the inner membrane, which contains components of the electron transport chain and ATPase. Physical activity requires ATP resynthesis, which is provided by different types of energyreleased reactions. An important part of our review reveals the analysis of data on the mitochondria themselves, which are key determinants of the functional state of the body’s cells during physical activity. Functional consequences of the changes in mitochondrial structure are of quite importance, especially associated with different configurations of mitochondria and mitochondrium − increasing the number of organelles and crystal density, normalization of the structural and functional state of the inner membrane and its protection from oxidative stress which is inherent in the intense physical activity. Oxidative stress and working hypoxia are a very common cause of further metabolic disorders, even before the formation of hypertrophy of chronic physical exertion, when you have to remove an athlete from the training process. In general, numerous metabolic processes in the myocardium, which are inherent in intense physical activity, undergo negative changes with further activation of athletes. One of the ways to correct energy deficiency caused by prolonged and intense physical activity should be the use of exogenous or endogenous substances involved in energy metabolism.
  • Specialized nutrition for athletes: evaluation of ergogenic action using the principles of evidence-based medicine
    L. M. Gunina, Ye. B. Shustov, I. F. Belenichev, N. L. Vysochina, R. V. Golovashchenko, et al.
    Pharmacia, 2022
    The aim is to form a balanced position regarding the ergogenic characteristics of new sports food products in the modern practice of training highly qualified athletes on the basis of clinical-experimental evaluation of the effectiveness of special purpose real food additives. Materials and methods. Studies were carried out on laboratory animals (36 Wistar rats at the age of 3 months weighing 150–200 g and 16 Svetlogorsk mini-pigs) as well as on 102 high-qualified representatives of cyclic sports (all men aged 19–26) − members of national teams such as swimming (25), cross-country athletics (53), and skiing (24). As a control group, we studied 25 athletes of the same gender (all men), age and qualifications who received a placebo (starch capsules) for a long time during exercise. Placebo-controlled studies have been conducted on laboratory animals and athletes in compliance with the principles of bioethics. Results. It has been established that in the experiment the application of a new specialized food product MioActiv has a positive effect on the physical performance of animals and delays the onset of fatigue, which significantly exceeds the corresponding characteristics in the placebo group. Athletes also showed an increase in speed when passing competitive distances by 18.7–21.4% (p < 0,05), as well as a significant increase in mental endurance indicators (p < 0,05). The approbation of the innovative pre-workout pharmacological nutrient complex Pre-Fuse also showed the high efficacy and safety of this product with a positive effect of increasing the performance and endurance of animals, as well as the parameters of mental performance of athletes in the main groups, in contrast to the data in the placebo control groups. Conclusions. An analysis of the prescription components of new types of ergogenic sports nutrition and the presented results of preclinical and clinical studies conducted on the basis of the evidence-based medicine paradigm regarding the effectiveness of specialized products indicate the complex action of these funds on various aspects of both physical and mental performance. From the materials presented in the article, it can be seen that specialized sports nutrition products of an ergogenic orientation can provide significant assistance in increasing sports results.
  • Oxidative stress as a factor in the deterioration of oxygen transfer during exercise
    L.M. Gunina, I.L. Rybina, Yu.A. Ataman, V.L. Voitenko, and
    Fiziologichnyi Zhurnal, 2021
    Blood oxygen transport regulation by physical activity increase within training dynamics is provided with different mechanisms: from the quantitative and qualitative erythron restructure (including endogenous erythropoietin rise and main erythrocyte index shifts) to change in haemoglobin affinity to oxygen, its heterogeneous structure and blood flow growth as a result of endothelium hyperpolarisation. However, the erythrocyte itself remains a key performer in blood velocity control, due to its structure and functions. This review summarizes the data of modern scientific literature on the characteristics of erythrocytes, which make these cells one of the key links in the oxygen transport system of the blood. The focus on this property of erythrocytes during physical activity is based on the fact that the athlete’s muscles must be supplied with enough oxygen to ensure high performance. Specific training and extra-training factors affecting the content of erythrocytes have been determined. The membrane structure is treated as a significant erythrocyte part in determining its deformation and microvascular blood transport. Enzymes associated with the erythrocyte membrane and affecting cell viability and performance are described. Besides, it is stressed on monitoring erythrocyte indices via modern equipment and assessing lipid peroxidation, which leads to disorders in erythrocyte membrane structure and functions.
  • APPROACHES TO PHARMACOLOGICAL CORRECTION OF PSYCHOPHYSIOLOGICAL STRESS IN ATHLETES
    Georgian Medical News, 2021
  • RESTRUCTURING OF SKELETAL MUSCLE, LUNG AND HEART TISSUES OF RATS UNDER HYPOXIA TRAINING
    K.V. Rosova, T.V. Bolgova, K.R. Tymoshenko, Ju.D. Vinnichuck, L.M. Gunina, et al.
    Fiziolohichnyi Zhurnal Kiev Ukraine 1994, 2016
  • Vascular endothelial growth factor during physical loads with different mechanism of energy's providing of muscular work
    Ju.D. Vinnichuck, L.M. Gunina, and
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2015
  • [Tissue hypoxia and related metabolic changes of homeostasis during vibration load in athletes]
    LM Hunina, IuD Vinnychuk, and
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2014
  • [Effectiveness of use of omega-3 polyunsaturated fatty acids at physical loads].
    LM Hunina, IS Chekman, TIu Nebesna, NO Horchakova, and
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2013
  • [Effect of sodium succinate on oxidative stress in erythrocytes during physical loading].
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2011
  • The role of prophylaxis and correction of functional anemia during increased physical activity in sportsmen
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2007
  • Biochemical and functional characteristics of erythrocyte membranes in sportsmens with anemia
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2007
  • Investigation of mechanisms of ceruloplasmin interaction with monomolecular distearoyl phosphatidylcholine films
    Ukrain Skyi Biokhimichnyi Zhurnal, 2007
  • The effect of rithmocor on the structural and functional state of erythrocyte membranes and athlete's adaptation to intensive training loads
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2006
  • Oxidative stress and its role in carcinogenesis
    Fiziolohichny Zhurnal Kiev Ukraine 1994, 2006
  • Membrane protective action of ceruloplasmine upon the use of doxorubicine in vivo
    Experimental Oncology, 2002
  • Efficacy and perspective of application of preparation based on hydrogel and xerogel of methyl-silicic acid in patients with the intestinal malignancy
    Klinicheskaya Khirurgiya, 2001
  • New approach to the treatment of primary malignant non-Hodgkin's lymphoma of the stomach
    Klinichna Khirurhiia Ministerstvo Okhorony Zdorov Ia Ukrainy Naukove Tovarystvo Khirurhiv Ukrainy, 1996
  • The clinical and biochemical characteristics of the status of stomach cancer patients who were exposed to ionizing radiation as a result of the accident at the Chernobyl Atomic Electric Power Station
    Likars Ka Sprava Ministerstvo Okhorony Zdorov Ia Ukrainy, 1995
  • The antistressor action of dibunol in surgical stress in lung cancer patients
    Eksperimental Naia I Klinicheskaia Farmakologiia, 1994
  • The prevention of surgical stress in lung cancer patients with the antioxidant dibunol
    Likars Ka Sprava Ministerstvo Okhorony Zdorov Ia Ukrainy, 1994
  • Use of ultraviolet irradiation of the blood after surgery in patients with tumors
    Klinicheskaya Khirurgiya, 1992
  • Biochemical criteria of early diagnosis of postoperative pancreatitis in oncologic patients
    Klinicheskaya Khirurgiya, 1991
  • Use of the indices of kallikrein-kinin system of the blood for evaluating the effectiveness of prophylaxis and treatment of postoperative purulent complications in oncologic patients
    Klinicheskaya Khirurgiya, 1989
  • The kinin system of blood and myocardium in controlled restriction of coronary circulation
    Voprosy Meditsinskoj Khimii, 1981
  • Kallikrein-kinin system and adenosine metabolism system of blood and heart and their changes at hypothalamic-hypophyseal stimulation in rabbits of different age
    Vladimir V. Frolkis, Rachel A. Frolkis, Bronislava V. Pugach, Larisa M. Gunina, Yuri E. Rushkevich
    Gerontology, 1980
  • Alterations in blood kallikrein-kinine system in dynamics of experimental myocardial infarction
    Voprosy Meditsinskoj Khimii, 1978
  • Kinin forming enzymes and kininogen in blood after experimental myocardial infarction in dogs (Russian)
    Fiziologicheskii Zhurnal, 1977

Publications

1. Gunina LM, Vysochina NL, Danylchenko SI, Mikhalyuk EL, Voitenko VL. Approaches to pharmacological correction of psychophysiological stress in athletes. Georgian Medical News. 2021;7–8(316–317):158–164.
2. Gunina LM, Shustov YeB, Belenichev IF, Vysochina NL, Golovashchenko RV, Morozova OV. Specialized nutrition for athletes: evaluation of ergogenic action using the principles of evidence-based medicine. Pharmacia. 2022;69(1):37–44.
3. Гуніна ЛМ, Бєленічев ІФ, Атаман ЮО, Войтенко ВЛ, Безугла ВВ. Енергозабезпечення серця та скелетних м’язів за фізичних навантажень: мітохондріальний вектор (огляд літератури). Фізіологічний журнал. 2022;68(5):67–78. (Україна)
4. Бєленічев ІФ, Алієва ОГ, Гуніна ЛМ, Бухтіярова НВ. Оцінка ефективності медикаментозної нейропротекції після експериментальної пренатальної гіпоксії. Фізіологічний журнал. 2023;69(1):43–53. (Україна)
5. Belenichev IF, Gunina LM, Orlov OI, Doroshenko EYu, Danylchenko SI, Skoryna DYu. The role of lactate in the body of martial artists during physical activity: a new look at the problem. Modern Medical Technology. 2025;17(1):51–59.