I am a microbiologist and microbial ecologist interested in understanding how microorganisms and their interactions shape biological processes across diverse environments, from marine ecosystems to host-associated systems. My research focuses on microbial interactions, symbiosis, and the molecular mechanisms underlying complex ecological relationships.
My work spans surface-associated microbial communities, biofilms, and host–microbe interactions, with relevance to environmental, industrial, and clinical contexts, and more recently explores microbial symbiosis in Antarctic marine sponges. I am an active member of the Early Career Scientist Committee of the International Society for Microbial Ecology - ISME, where I serve as Media Manager, contributing to the visibility of early career researchers and fostering an inclusive international scientific community.
EDUCATION
I hold an Engineering degree and a Master’s in Biotechnology from Universidad Andrés Bello, and a PhD in Biological Sciences (Molecular Genetics and Microbiology) from the Pontificia Universidad Católica de Chile.
RESEARCH, TEACHING, or OTHER INTERESTS
Microbiology, Ecology, Applied Microbiology and Biotechnology, Biochemistry, Genetics and Molecular Biology
13
Scopus Publications
273
Scholar Citations
8
Scholar h-index
8
Scholar i10-index
Scopus Publications
Transcriptomic Insights Into the Immune Repertoire of an Antarctic Sponge Leslie K. Daille, Mario Moreno‐Pino, Eduardo Hajdu, Nicole Trefault Ecology and Evolution, 2025 Antarctic marine sponges are essential components of the benthic fauna, playing a crucial role both through their own biological activities and their symbiotic relationships with diverse microorganisms. Yet, the transcriptional repertoire and the immune responses associated with interactions with microorganisms in this unique environment still need to be fully understood. Here, we investigated the transcriptional repertoire underlying the immune system processes of the Antarctic sponge Myxilla ( Burtonanchora ) lissostyla . We generated a de novo transcriptome and functional annotation for M. ( B. ) lissostyla , collected during the austral summer of 2019, 2020, and 2021. Our findings revealed an extensive transcriptional repertoire with a high and consistent expression of constitutive transcripts across the years. Key pathways related to immune response and homeostasis were the most expressed in the Antarctic sponge transcriptome, and a diverse array of immune receptors highlights the wide host immune repertoire. Low microbial abundance sponges share a vast repertoire of immune receptors, and a predominance of membrane‐bound PRRs was detected mainly in M . ( B. ) lissostyla , indicating a broad range of receptors available for initial interactions and engagement with microorganisms. The functional repertoire unveiled here establishes baselines for assessing potential functional changes that may arise due to climate change.
Antibiofilm Effects of Modifying Polyvinylidene Fluoride Membranes with Polyethylenimine, Poly(acrylic acid) and Graphene Oxide Mario Castillo-Ruiz, Constanza Negrete, Juan Pablo Espinoza, Iván Martínez, Leslie K. Daille, et al. Polymers, 2024 Biofouling in membrane filtration systems poses significant operational challenges, leading to decreased permeate flux. The aim of this work was to study the anti-biofilm properties of new nanofiltration membranes produced via layer-by-layer, LBL, assembly by coating a polyvinylidene fluoride (PVDF) support with a polyethylenimine (PEI) and poly(acrylic acid)/graphene oxide (PAA-GO) mixture. The membranes were characterized according to contact angle, scanning electron microscopy (SEM), atomic force microscopy and their Z-potential. Biofilm quantification and characterization were carried out using crystal violet staining and SEM, while bacterial viability was assessed by using colony-forming units. The membrane with three bilayers ((PAA-PEI)3/PVDF) showed a roughness of 77.78 nm. The incorporation of GO ((GO/PAA-PEI)3/PVDF) produced a membrane with a smoother surface (roughness of 26.92 nm) and showed salt rejections of 16% and 68% for NaCl and Na2SO4, respectively. A significant reduction, ranging from 82.37 to 77.30%, in biofilm formation produced by S. aureus and E. coli were observed on modified membranes. Additionally, the bacterial viability on the modified membranes was markedly reduced (67.42–99.98%). Our results show that the modified membranes exhibited both antibiofilm and antimicrobial capacities, suggesting that these properties mainly depend on the properties of the modifying agents, as the initial adherence on the membrane surface was not totally suppressed, but the proliferation and formation of EPSs were prevented.
Electrochemical bacterial enrichment from natural seawater and its implications in biocorrosion of stainless-steel electrodes María José De La Fuente, Leslie K. Daille, Rodrigo De la Iglesia, Magdalena Walczak, Francisco Armijo, et al. Materials, 2020 Microbial electrochemical technologies have revealed the opportunity of electrochemical enrichment for specific bacterial groups that are able to catalyze reactions of interest. However, there are unsolved challenges towards their application under aggressive environmental conditions, such as in the sea. This study demonstrates the impact of surface electrochemical potential on community composition and its corrosivity. Electrochemical bacterial enrichment was successfully carried out in natural seawater without nutrient amendments. Experiments were carried out for ten days of exposure in a closed-flow system over 316L stainless steel electrodes under three different poised potentials (−150 mV, +100 mV, and +310 mV vs. Ag/AgCl). Weight loss and atomic force microscopy showed a significant difference in corrosion when +310 mV (vs. Ag/AgCl) was applied in comparison to that produced under the other tested potentials (and an unpoised control). Bacterial community analysis conducted using 16S rRNA gene profiles showed that poised potentials are more positive as +310 mV (vs. Ag/AgCl) resulted in strong enrichment for Rhodobacteraceae and Sulfitobacter. Hence, even though significant enrichment of the known electrochemically active bacteria from the Rhodobacteraceae family was accomplished, the resultant bacterial community could accelerate pitting corrosion in 316 L stainless steel, thereby compromising the durability of the electrodes and the microbial electrochemical technologies.
Effect of tidal cycles on bacterial biofilm formation and biocorrosion of stainless steel AISI 316L Leslie K. Daille, Javiera Aguirre, Diego Fischer, Carlos Galarce, Francisco Armijo, et al. Journal of Marine Science and Engineering, 2020 The effects of tidal cycles associated with the water level on the biocorrosion of stainless steel AISI 316L were studied. Steel coupons were exposed to different conditions of immersion in mesocosms fed by fresh seawater either continuously or in accordance with the periodicity of natural tides. After 5 and 15 weeks, all coupons were found to have undergone ennoblement associated with the formation of a biofilm. Analysis of the composition of the bacterial community using denaturing gradient gel electrophoresis (DGGE) revealed differences in the biological succession. After 15 weeks, exposure to the simulated tidal conditions resulted in biofilms with lesser bacterial richness; the corresponding rate of corrosion, as determined by weight loss, was about 40 times lower compared to the case for the continuous exposure to seawater. Phylogenetic analysis of selected DGGE bands and the inspection of biofilm morphologies revealed that the faster rate of corrosion was associated with the presence of iron-oxidizing Zetaproteobacteria and eukaryotic photosynthetic microorganisms. On the other hand, intermittent exposure to seawater resulted in the succession of microorganisms resistant to the stress associated with sudden environmental changes, which was associated with a low rate of corrosion.
Carbonate-rich dendrolitic cones: Insights into a modern analog for incipient microbialite formation, Little Hot Creek, Long Valley Caldera, California James A. Bradley, Leslie K. Daille, Christopher B. Trivedi, Caitlin L. Bojanowski, Blake W. Stamps, et al. Npj Biofilms and Microbiomes, 2017 Ancient putative microbial structures that appear in the rock record commonly serve as evidence of early life on Earth, but the details of their formation remain unclear. The study of modern microbial mat structures can help inform the properties of their ancient counterparts, but modern mineralizing mat systems with morphological similarity to ancient structures are rare. Here, we characterize partially lithified microbial mats containing cm-scale dendrolitic coniform structures from a geothermal pool (“Cone Pool”) at Little Hot Creek, California, that if fully lithified, would resemble ancient dendrolitic structures known from the rock record. Light and electron microscopy revealed that the cm-scale ‘dendrolitic cones’ were comprised of intertwined microbial filaments and grains of calcium carbonate. The degree of mineralization (carbonate content) increased with depth in the dendrolitic cones. Sequencing of 16S rRNA gene libraries revealed that the dendrolitic cone tips were enriched in OTUs most closely related to the genera Phormidium, Leptolyngbya, and Leptospira, whereas mats at the base and adjacent to the dendrolitic cones were enriched in Synechococcus. We hypothesize that the consumption of nutrients during autotrophic and heterotrophic growth may promote movement of microbes along diffusive nutrient gradients, and thus microbialite growth. Hour-glass shaped filamentous structures present in the dendrolitic cones may have formed around photosynthetically-produced oxygen bubbles—suggesting that mineralization occurs rapidly and on timescales of the lifetime of a bubble. The dendrolitic-conical structures in Cone Pool constitute a modern analog of incipient microbialite formation by filamentous microbiota that are morphologically distinct from any structure described previously. Thus, we provide a new model system to address how microbial mats may be preserved over geological timescales.
Transcriptomic Insights Into the Immune Repertoire of an Antarctic Sponge LK Daille, M Moreno‐Pino, E Hajdu, N Trefault Ecology and Evolution 15 (12), e72786 , 2025 2025
Antibiofilm Effects of Modifying Polyvinylidene Fluoride Membranes with Polyethylenimine, Poly (acrylic acid) and Graphene Oxide M Castillo-Ruiz, C Negrete, JP Espinoza, I Martínez, LK Daille, ... Polymers 16 (23), 3418 , 2024 2024 Citations: 6
Seasonal variation in the biological succession of marine diatoms over 316L stainless steel in a coastal environment of Chile LK Daille, JR Spear, I Beech, IT Vargas, R De la Iglesia Biofouling 40 (1), 1-13 , 2024 2024 Citations: 3
Antibacterial activity of a complex bacteriocin secreted by Staphylococcus epidermidis against Porphyromonas gingivalis M Castillo-Ruiz, LK Daille, P Machuca, M Bittner Archives of Oral Biology 152, 105730 , 2023 2023 Citations: 3
When material science meets microbial ecology: Bacterial community selection on stainless steels in natural seawater LK Daille, J Aguirre, J Anguita, C Galarce, L Caro-Lara, F Armijo, ... Colloids and Surfaces B: Biointerfaces 221, 112955 , 2023 2023 Citations: 4
Testing the test: a comparative study of marine microbial corrosion under laboratory and field conditions C Canales, C Galarce, F Rubio, F Pineda, J Anguita, R Barros, ... ACS omega 6 (20), 13496-13507 , 2021 2021 Citations: 12
Electrochemical bacterial enrichment from natural seawater and its implications in biocorrosion of stainless-steel electrodes MJ De La Fuente, LK Daille, R De la Iglesia, M Walczak, F Armijo, ... Materials 13 (10), 2327 , 2020 2020 Citations: 11
Effect of tidal cycles on bacterial biofilm formation and biocorrosion of stainless steel AISI 316L LK Daille, J Aguirre, D Fischer, C Galarce, F Armijo, GE Pizarro, ... Journal of Marine Science and Engineering 8 (2), 124 , 2020 2020 Citations: 24
Influencia de colonizadores primarios e interacciones microbianas en el desarrollo de biocorrosión marina sobre acero inoxidable LK Daille https://repositorio.uc.cl/handle/11534/22405 , 2019 2019 Citations: 2
Study of poly (3, 4-ethylendioxythiphene) as a coating for mitigation of biocorrosion of AISI 304 stainless steel in natural seawater J Aguirre, L Daille, DA Fischer, C Galarce, G Pizarro, I Vargas, M Walczak, ... Progress in Organic Coatings 113, 175-184 , 2017 2017 Citations: 35
Carbonate-rich dendrolitic cones: insights into a modern analog for incipient microbialite formation, Little Hot Creek, Long Valley Caldera, California JA Bradley, LK Daille, CB Trivedi, CL Bojanowski, BW Stamps, ... npj Biofilms and Microbiomes 3 (1), 32 , 2017 2017 Citations: 35
Corrosion of stainless steel in simulated tide of fresh natural seawater of south east pacific DA Fischer, L Daille, J Aguirre, C Galarce, F Armijo, R De la Iglesia, ... International Journal of Electrochemical Science 11 (8), 6873-6885 , 2016 2016 Citations: 20
Vertical Microbial Community Variability of Carbonate-based Cones may Provide Insight into Formation in the Rock Record C Bojanowski, C Trivedi, LK Daille, J Bradley, H Johnson, BW Stamps, ... 2015 AGU Fall Meeting , 2015 2015
Evaluación in vitro del efecto de bactiófagos específicos para Fusobacterium nucleatum en biopelículas formadas sobre distintos tejidos del diente LK Daille http://repositorio.unab.cl/xmlui/handle/ria/3857 , 2012 2012
Co-detección de Patógenos Periodontales en Pacientes Chilenos con Periodontitis Crónica CM Troncoso, M Castillo-Ruiz, LK Daille, IA Fuentevilla, M Bittner Revista clínica de periodoncia, implantología y rehabilitación oral 3 (3 … , 2010 2010 Citations: 15
Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum P Machuca, L Daille, E Vinés, L Berrocal, M Bittner Applied and environmental microbiology 76 (21), 7243-7250 , 2010 2010 Citations: 103
MOST CITED SCHOLAR PUBLICATIONS
Isolation of a Novel Bacteriophage Specific for the Periodontal Pathogen Fusobacterium nucleatum P Machuca, L Daille, E Vinés, L Berrocal, M Bittner Applied and environmental microbiology 76 (21), 7243-7250 , 2010 2010 Citations: 103
Study of poly (3, 4-ethylendioxythiphene) as a coating for mitigation of biocorrosion of AISI 304 stainless steel in natural seawater J Aguirre, L Daille, DA Fischer, C Galarce, G Pizarro, I Vargas, M Walczak, ... Progress in Organic Coatings 113, 175-184 , 2017 2017 Citations: 35
Carbonate-rich dendrolitic cones: insights into a modern analog for incipient microbialite formation, Little Hot Creek, Long Valley Caldera, California JA Bradley, LK Daille, CB Trivedi, CL Bojanowski, BW Stamps, ... npj Biofilms and Microbiomes 3 (1), 32 , 2017 2017 Citations: 35
Effect of tidal cycles on bacterial biofilm formation and biocorrosion of stainless steel AISI 316L LK Daille, J Aguirre, D Fischer, C Galarce, F Armijo, GE Pizarro, ... Journal of Marine Science and Engineering 8 (2), 124 , 2020 2020 Citations: 24
Corrosion of stainless steel in simulated tide of fresh natural seawater of south east pacific DA Fischer, L Daille, J Aguirre, C Galarce, F Armijo, R De la Iglesia, ... International Journal of Electrochemical Science 11 (8), 6873-6885 , 2016 2016 Citations: 20
Co-detección de Patógenos Periodontales en Pacientes Chilenos con Periodontitis Crónica CM Troncoso, M Castillo-Ruiz, LK Daille, IA Fuentevilla, M Bittner Revista clínica de periodoncia, implantología y rehabilitación oral 3 (3 … , 2010 2010 Citations: 15
Testing the test: a comparative study of marine microbial corrosion under laboratory and field conditions C Canales, C Galarce, F Rubio, F Pineda, J Anguita, R Barros, ... ACS omega 6 (20), 13496-13507 , 2021 2021 Citations: 12
Electrochemical bacterial enrichment from natural seawater and its implications in biocorrosion of stainless-steel electrodes MJ De La Fuente, LK Daille, R De la Iglesia, M Walczak, F Armijo, ... Materials 13 (10), 2327 , 2020 2020 Citations: 11
Antibiofilm Effects of Modifying Polyvinylidene Fluoride Membranes with Polyethylenimine, Poly (acrylic acid) and Graphene Oxide M Castillo-Ruiz, C Negrete, JP Espinoza, I Martínez, LK Daille, ... Polymers 16 (23), 3418 , 2024 2024 Citations: 6
When material science meets microbial ecology: Bacterial community selection on stainless steels in natural seawater LK Daille, J Aguirre, J Anguita, C Galarce, L Caro-Lara, F Armijo, ... Colloids and Surfaces B: Biointerfaces 221, 112955 , 2023 2023 Citations: 4
Seasonal variation in the biological succession of marine diatoms over 316L stainless steel in a coastal environment of Chile LK Daille, JR Spear, I Beech, IT Vargas, R De la Iglesia Biofouling 40 (1), 1-13 , 2024 2024 Citations: 3
Antibacterial activity of a complex bacteriocin secreted by Staphylococcus epidermidis against Porphyromonas gingivalis M Castillo-Ruiz, LK Daille, P Machuca, M Bittner Archives of Oral Biology 152, 105730 , 2023 2023 Citations: 3
Influencia de colonizadores primarios e interacciones microbianas en el desarrollo de biocorrosión marina sobre acero inoxidable LK Daille https://repositorio.uc.cl/handle/11534/22405 , 2019 2019 Citations: 2
Transcriptomic Insights Into the Immune Repertoire of an Antarctic Sponge LK Daille, M Moreno‐Pino, E Hajdu, N Trefault Ecology and Evolution 15 (12), e72786 , 2025 2025
Vertical Microbial Community Variability of Carbonate-based Cones may Provide Insight into Formation in the Rock Record C Bojanowski, C Trivedi, LK Daille, J Bradley, H Johnson, BW Stamps, ... 2015 AGU Fall Meeting , 2015 2015
Evaluación in vitro del efecto de bactiófagos específicos para Fusobacterium nucleatum en biopelículas formadas sobre distintos tejidos del diente LK Daille http://repositorio.unab.cl/xmlui/handle/ria/3857 , 2012 2012