Lucas Henrique da Silva e Silva

@gov.br

Post graduate student of the Pos-Graduate Program in Parasite Biology in the Amazon
Instituto Evandro Chagas

Lucas Henrique da Silva e Silva


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https://researchid.co/lucashenrique

RESEARCH, TEACHING, or OTHER INTERESTS

Genetics, Biochemistry, Genetics and Molecular Biology, Virology, Microbiology
3

Scopus Publications

Scopus Publications

  • Coding-complete genome sequence of a divergent member of the genus Gordisvirus detected in Sabethes (Peytonulus) undosus Coquillet mosquitoes (Diptera: Culicidae) from Brazil
    Daniel Dias, Bruna Nascimento, Ana Cruz, Sandro Silva, Lúcia Reis, et al.
    Microbiology Resource Announcements, 2025
    We report the complete genome sequence of a divergent member of the genus Gordisvirus (family Xinmoviridae , order Mononegavirales ), obtained through metagenomic sequencing of Sabethes ( Peytonulus ) undosus Coquillett mosquitoes in the Brazilian Amazon. Phylogenetic analyses confirmed its classification. The genome comprises 12,150 nucleotides and encodes five open-reading frames.
  • Sequencing and Description of the Mitochondrial Genome of Orthopodomyia fascipes (Diptera: Culicidae)
    Fábio Silva da Silva, Bruna Laís Sena do Nascimento, Ana Cecília Ribeiro Cruz, Sandro Patroca da Silva, Carine Fortes Aragão, et al.
    Genes, 2024
    The genus Orthopodomyia Theobald, 1904 (Diptera: Culicidae) comprises 36 wild mosquito species, with distribution largely restricted to tropical and temperate areas, most of which are not recognized as vectors of epidemiological importance due to the lack of information related to their bionomy and involvement in the cycle transmission of infectious agents. Furthermore, their evolutionary relationships are not completely understood, reflecting the scarcity of genetic information about the genus. Therefore, in this study, we report the first complete description of the mitochondrial genome of a Neotropical species representing the genus, Orthopodomyia fascipes Coquillet, 1906, collected in the Brazilian Amazon region. Using High Throughput Sequencing, we obtained a mitochondrial sequence of 15,598 bp, with an average coverage of 418.5×, comprising 37 functional subunits and a final portion rich in A + T, corresponding to the control region. The phylogenetic analysis, using Maximum Likelihood and Bayesian Inference based on the 13 protein-coding genes, corroborated the monophyly of Culicidae and its two subfamilies, supporting the proximity between the tribes Orthopodomyiini and Mansoniini, partially disagreeing with previous studies based on the use of molecular and morphological markers. The information generated in this study contributes to a better understanding of the taxonomy and evolutionary history of the genus and other groups of Culicidae.
  • Genus Culex Linnaeus, 1758 (Diptera: Culicidae) as an Important Potential Arbovirus Vector in Brazil: An Integrative Review
    Lúcia Aline Moura Reis, Ana Beatriz Oliveira Pampolha, Bruna Lais Sena do Nascimento, Daniel Damous Dias, Pedro Arthur da Silva Araújo, et al.
    Life, 2023
    The genus Culex has 817 species subdivided into 28 subgenera. It has a cosmopolitan distribution, being most abundant in countries with a tropical climate. Understanding the ecology and diversity of viruses circulating in the species of this genus is important for understanding their role as arbovirus vectors in Brazil. To conduct an integrative review to identify the importance of the Culex genus as arbovirus vectors in Brazil. A search was carried out for scientific papers in the PubMed, BVSalud, Patuá-IEC and International Catalogue of Arboviruses: including certain other viruses of vertebrates databases. 36 publications describing arbovirus detections in Culex mosquitoes collected in the field in Brazil were evaluated. A total of 42 arbovirus species were detected, as well as studies analyzing the vector competence of C. quinquefasciatus for the transmission of four different arboviruses. The study of the Culex genus and its role as a vector of arboviruses in Brazil is essential for understanding transmission cycles, with the main aim of reducing cases of human infection. Thus, entomovirological surveillance guides the implementation of actions to detect circulating arboviruses among vectors to anticipate measures aimed at preventing or reducing the risk of arbovirus outbreaks in the country.