TNF superfamily member 14 drives post-influenza depletion of alveolar macrophages, enabling secondary pneumococcal pneumonia Anoop Arunagiri, Leena Haataja, Maroof Alam, Noah F. Gleason, Emma Mastroianni, et al. Journal of Clinical Investigation, 2026 In pancreatic β cells, misfolded proinsulin is a substrate for ER-associated protein degradation (ERAD) via HRD1/SEL1L. Alternately, β cell HRD1 activity is reported to improve, or impair, insulin biogenesis. Further, while β cell SEL1L deficiency causes HRD1 hypofunction and diminishes islet insulin content, reports conflict as to whether β cell ERAD deficiency increases or decreases proinsulin levels. Here, we examined β cell–specific Hrd1 -KO mice (chronic deficiency) and rodent (and human islet) β cells treated acutely with HRD1 inhibitor. β -Hrd1 –KO mice developed diabetes with decreased islet proinsulin, yet a relative increase of misfolded proinsulin redistributed to the ER. They also showed upregulated biochemical markers of β cell ER stress and autophagy, electron microscopy evidence of ER enlargement and decreased insulin granule content, and increased glucagon-positive islet cells. Misfolded proinsulin was also increased in islets treated with inhibitors of lysosomal degradation. Preceding any loss of total proinsulin, acute HRD1 inhibition triggered increased nonnative proinsulin, increased phospho-eIF2α with inhibited proinsulin synthesis, and increased LC3b-II (the abundance of which requires expression of ΣR1). We posit a subset of proinsulin molecules undergo HRD1-mediated disposal. When HRD1 is unavailable, misfolded proinsulin accumulates, accompanied by increased phospho-eIF2α that limits further proinsulin synthesis, plus ΣR1-dependent autophagy activation, ultimately lowering steady-state β cell proinsulin (and insulin) levels and triggering diabetes.
Infectious bursal disease virus (IBDV) as a novel oncolytic virotherapy in glioblastoma Vicent Tur-Planells, Yonina Bykov, Gloria Dawodu, Noemi García-Romero, Sara Izpura-Luis, et al. Journal for Immunotherapy of Cancer, 2025 Background Glioblastoma (GBM) is the most aggressive form of cancer of the central nervous system. Despite advances in immunotherapies and standard-of-care treatments for GBMs, clinical outcomes remain limited—owing to the immunosuppressive tumor microenvironment and the intrinsic resistance of GBM to conventional approaches. As a result, there is growing interest in rational combination strategies, particularly those pairing oncolytic viruses with immune-based therapies or established treatment modalities. Oncolytic viruses, by displaying conditionally enabled tumor cell-restricted replication, while stimulating antitumor immune responses and leaving healthy tissue unharmed, have the potential to reshape the therapeutic landscape in GBM and aid in achieving more durable benefits for patients. This study investigates the use of infectious bursal disease virus (IBDV) as a potential virotherapy for GBM. Methods and results In vitro, IBDV infects and replicates within murine GBM cells and patient-derived GBM stem cells, inducing direct oncolysis and activating proinflammatory gene expression programs. IBDV also enhances the cytolytic activity of temozolomide (TMZ) in treated GBM cells, complementing TMZ chemotherapeutic activity. In vivo, treatment with IBDV in CT-2A GBM-bearing syngeneic mice significantly reduced tumor growth and improved survival compared with control mice. Intratumoral administration of IBDV induces a deep remodeling of the tumor immune microenvironment, reducing immunosuppressive M2-like macrophages and increasing the ratio of CD8+T cells to regulatory T cells. This reversion of immunosuppression linked to monocyte-derived macrophages has been confirmed on experimental ex vivo infections of explants derived from human GBM donors. Conclusion These findings support further consideration of IBDV as a novel virotherapeutic agent for GBM.
A bioactive soluble recombinant mouse LIGHT promotes effective tumor immune cell infiltration delaying tumor growth Maria-Luisa del Rio, Oscar-Mariano Nuero-Garcia, Giovanna Roncador, Raquel Garcimartín-Bailon, Juan-Carlos Cubria, et al. Journal of Molecular Medicine, 2025 The TNF family member LIGHT (TNFSF14) binds to two receptors, HVEM (TNFSFR14) and LTβR (TNFSFR3). HVEM functions as a costimulatory molecule, whereas LTβR is involved in the development of lymph nodes and ectopic tertiary lymphoid structures at chronic inflammation sites. The classical approach of fusing soluble recombinant proteins to the Fc fragment of IgG resulted in a functionally inactive Ig.mouse (m) LIGHT protein. However, in line with the fact that TNF family members cluster receptors as trimers, addition of a small homotrimeric domain (foldon) N-terminal of mLIGHT produced an Ig.Foldon-mLIGHT protein able to bind and engage HVEM and LTβR in a cell-based reporter bioassay. In the tumor model of B16.F10 melanoma cells implanted into syngeneic recipients, cells transduced with membrane-bound mLIGHT grew as aggressively as mock-transduced cells, but growth of tumors of B16.F10 cells expressing Ig.Foldon-mLIGHT was delayed and characterized by significant immune infiltration of dendritic cells and cytotoxic cells. This work unveils the potential of active soluble LIGHT, as a single agent, to recruit cytotoxic cells and dendritic cells at the tumor site to inhibit tumor growth. This effect may be further enhanced with immune checkpoint blockade therapies. Key messages The classical approach of fusing soluble recombinant proteins to the Fc fragment of IgG resulted in a functionally inactive Ig.mouse (m) LIGHT (TNFSF14) protein. The addition of a small homotrimeric domain (foldon) N-terminal of mouse LIGHT produces a proper folded bioactive mouse LIGHT recombinant protein. Constitutive intratumor expression of secreted Ig-Foldon-LIGHT, but not membrane LIGHT, delays tumor growth. Tumors secreting LIGHT, as a single agent, promote beneficial anti-tumor responses through the recruitment and infiltration of cytotoxic cells and dendritic cells. Graphical Abstract The intrinsic source of native soluble LIGHT and LTα1β2 (produced by neutrophils and activated T cells and NK cells) along with the tumor secreting recombinant Ig.Foldon-LIGHT co-stimulate NK and T cells through HVEM and license DC for antigen presentation to promote anti-tumor T cell responses in the tumor draining lymph nodes. Furthermore, native LIGHT produced by neutrophils, NK, and T cells and recombinant LIGHT along with LTα1β2 secreted by recruited B cells (LTi, lymphoid tissue inducer cells) would together activate stromal cells of the tumor microenvironment (TME) through LTβR (LTo, lymphoid tissue organizing cells) to release chemokines driving endothelial activation and the upregulation of adhesion molecules that in turn would facilitate transmigration of immune cells from the blood vessels to the tumor site. It would also condition myeloid cells expressing LTβR to secrete cytokines such as TGF-beta that contribute to blood vessel normalization.
Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain) Sara González, Rosario Panadero, María Luisa Del Rio, María Natividad Díez, María del Rosario Hidalgo, et al. Veterinary Research Communications, 2024 Hypodermosis in Cervus elaphus was studied in the Riaño Regional Hunting Reserve, Province of León, north-western Spain. One hundred and ten red deer were examined for the presence of warble fly larvae. They were analyzed by PCR analysis of the COI region of mt-DNA and identified as Hypoderma actaeon. The prevalence of larvae was 42.7% with a mean intensity of 12.5 ± 18 (range 1–80) warbles/deer infested. The distribution of larvae in the infested animals showed an aggregated/overdispersed pattern (aggregation index = 25.84), where the larvae are not randomly or uniformly distributed, but strongly aggregated among their hosts. Larvae were found in all three states. First and second-instars were observed mainly in the autumn until the end of winter (November-March) and third-instars in late winter until mid-spring (March–May). The adult animals and the males had a higher prevalence than the young and the females, finding statistically significant differences only according to the sex of the animals. Seasonal variations were observed in the prevalence with the highest number of infested animals in winter and autumn, but not in terms of the mean intensity of parasites. Additionally, we assessed the presence of anti-Hypoderma antibodies in serum by means of indirect ELISA tests, using a crude larval extract (CLE) and a purified fraction the hypodermin C (HyC) obtained from first instars of Spanish isolates of Hypoderma lineatum (cattle). These findings confirm that H. actaeon is widely distributed in northern Spain, and provide new information about its chronobiology in mountainous Atlantic ecosystems from southwestern Europe.
Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer (Capreolus capreolus) from the Province of León, Spain: An Epidemiological and Molecular Study Sara González, María Luisa del Rio, Natividad Díez-Baños, Angélica Martínez, María del Rosario Hidalgo Animals, 2023 A study of gastrointestinal nematodes in roe deer was carried out in the regional hunting reserves of Riaño and Mampodre, Province of León, Spain, to provide information on their prevalence and intensity of infection in relation to the sampling areas, age of the animals, and body weight. Through a regulated necropsy of the animals, all of them harbored gastrointestinal nematodes in their digestive tract, with a mean intensity of parasitism of 638 ± 646.1 nematodes/infected animal. Eleven genera were found and 18 species of gastrointestinal nematodes were identified, three of them polymorphic: Trichostrongylus axei, Trichostrongylus vitrinus, Trichostrongylus capricola, Trichostrongylus colubriformis, Haemonchus contortus, Spiculopteragia spiculoptera/Spiculopteragia mathevossiani, Ostertagia leptospicularis/Ostertagia kolchida, Ostertagia (Grosspiculopteragia) occidentalis, Teladorsagia circumcincta/Teladorsagia trifurcate, Marshallagia marshalli, Nematodirus europaeus, Cooperia oncophora, Capillaria bovis, Oesophagostomum venulosum, and Trichuris ovis. All of them have already been cited in roe deer in Europe, but Marshallagia marshalli, Capillaria bovis, and Ostertagia (Grosspiculopteragia) occidentalis are reported for the first time in Spain in this host. The abomasum was the intestinal section, where the prevalence (98.9%) and mean intensity (x¯ = 370.7 ± 374.4 worms/roe deer; range 3–1762) were significantly higher, but no statistically significant differences were found when comparing the sampling areas and age of animals. The animals with lower body weight had a higher parasite load than those in better physical condition, finding, in this case, statistically significant differences (p = 0.0020). Seven genera and 14 species were identified. In the small intestine, 88% of the animals examined presented gastrointestinal nematodes, with an average intensity of x¯ = 131.7 ± 225.6 parasites/infected animal, ranging between 4–1254 worms. No statistically significant differences were found when the three parameters studied were compared. Four genera and seven species were identified. In the large intestine/cecum, 78.3% of the examined roe deer presented adult worms, with an average intensity of 6.3 ± 5.5 worms/infected animal; range 1–26 worms. Only statistically significant differences were observed when considering the mean intensity of parasitism and the sampling area (p = 0.0093). Two genera and two species were identified. Several of the species found in the study were studied molecularly, and with the sequences obtained compared with those deposited in GenBank, phylogenetic trees were prepared to determine their taxonomic status. Using coprological techniques, the existing correlation in the shedding of gastrointestinal nematode eggs in roe deer was investigated with that of semi-extensive sheep farms in the same study area to verify the existence of cross-transmission of these parasites between wild and domestic animals. The high values found in the studied parameters show that northern Spain is an area of high-intensity infection for roe deer.
Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer (Cervus elaphus) from northern Spain: Microscopy study and molecular analysis Sara González, Maria Luisa Del Rio, Maria Natividad Diez, Maria del Rosario Hidalgo, Angelica Martínez Microscopy Research and Technique, 2023 Hypoderma spp. larvae were observed subcutaneously in the dorsal and lumbar regions of red deer (Cervus elaphus) hunted in the province of León (northwestern Spain) causing a myiasis. They were removed and initially classified by their size, shape, color, and location under the skin into the three larval stages that parasitize these animals. The morphological characteristics of the first and second‐instar are described and from the features of the third‐instar the species was identified as Hypoderma actaeon. To accurately identify this species, five isolates of genomic DNA from the third‐instar, two from the second‐instar and two from first‐instar of H. actaeon were analyzed by PCR analysis of the COI region of mt‐DNA. The results confirmed that the examined samples exactly matched with H. actaeon. This study has shown the morphological identification of the three larval stages of H. actaeon and, for the first time, the first and second‐instar larvae have been molecularly characterized. Finally, identification of only H. actaeon suggests that this species is the only affecting red deer in the Iberian Peninsula.
Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1- stem cell-like T cells over PD-1+ T cells curbing tumor progression Maria-Luisa del Rio, Carla Yago-Diez de Juan, Giovanna Roncador, Eduardo Caleiras, Ramón Álvarez-Esteban, et al. Frontiers in Immunology, 2023 IntroductionA high frequency of mutations affecting the gene encoding Herpes Virus Entry Mediator (HVEM, TNFRSF14) is a common clinical finding in a wide variety of human tumors, including those of hematological origin.MethodsWe have addressed how HVEM expression on A20 leukemia cells influences tumor survival and its involvement in the modulation of the anti-tumor immune responses in a parental into F1 mouse tumor model of hybrid resistance by knocking-out HVEM expression. HVEM WT or HVEM KO leukemia cells were then injected intravenously into semiallogeneic F1 recipients and the extent of tumor dissemination was evaluated.ResultsThe loss of HVEM expression on A20 leukemia cells led to a significant increase of lymphoid and myeloid tumor cell infiltration curbing tumor progression. NK cells and to a lesser extent NKT cells and monocytes were the predominant innate populations contributing to the global increase of immune infiltrates in HVEM KO tumors compared to that present in HVEM KO tumors. In the overall increase of the adaptive T cell immune infiltrates, the stem cell-like PD-1- T cells progenitors and the effector T cell populations derived from them were more prominently present than terminally differentiated PD-1+ T cells.ConclusionsThese results suggest that the PD-1- T cell subpopulation is likely to be a more relevant contributor to tumor rejection than the PD-1+ T cell subpopulation. These findings highlight the role of co-inhibitory signals delivered by HVEM upon engagement of BTLA on T cells and NK cells, placing HVEM/BTLA interaction in the spotlight as a novel immune checkpoint for the reinforcement of the anti-tumor responses in malignancies of hematopoietic origin.
Differential Engraftment of Parental A20 PD-L1 WT and PD-L1 KO Leukemia Cells in Semiallogeneic Recipients in the Context of PD-L1/PD-1 Interaction and NK Cell-Mediated Hybrid Resistance Maria-Luisa del Rio, Jose-Antonio Perez-Simon, Jose-Ignacio Rodriguez-Barbosa Frontiers in Immunology, 2022 The contribution of natural killer (NK) cells to tumor rejection in the context of programmed death-ligand 1/programmed death 1 (PD-L1/PD-1) blockade is a matter of intense debate. To elucidate the role of PD-L1 expression on tumor cells and the functional consequences of engaging PD-1 receptor on cytotoxic cells, PD-L1 expression was genetically inactivated and WT or PD-L1-deficient parental tumor cells were adoptively transferred intravenously into F1 recipients. The engraftment of PD-L1-deficient A20 tumor cells in the spleen and liver of F1 recipients was impaired compared with A20 PD-L1 WT tumor counterparts. To elucidate the mechanism responsible for this differential tumor engraftment and determine the relevance of the role of the PD-L1/PD-1 pathway in the interplay of tumor cells/NK cells, a short-term competitive tumor implantation assay in the peritoneal cavity of semiallogeneic F1 recipients was designed. The results presented herein showed that NK cells killed target tumor cells with similar efficiency regardless of PD-L1 expression, whereas PD-L1 expression on A20 tumor cells conferred significant tumor protection against rejection by CD8 T cells confirming the role of the co-inhibitory receptor PD-1 in the modulation of their cytotoxic activity. In summary, PD-L1 expression on A20 leukemia tumor cells modulates CD8 T-cell-mediated responses to tumor-specific antigens but does not contribute to inhibit NK cell-mediated hybrid resistance, which correlates with the inability to detect PD-1 expression on NK cells neither under steady-state conditions nor under inflammatory conditions.
The Role of the Inhibitory Ligand HVEM and Its Receptors CD160 and BTLA in the Regulation of Anti-retroviral T Cell Responses Paul David, Jaana Westmeier, Malgorzata Drabczyk-Pluta, Tanja Werner, Julia Ickler, et al. Frontiers in Virology, 2022 Specific CD8+ T cells are crucial for the control of viruses. However, during many chronic viral infections these cells become dysfunctional. Immune checkpoint receptors, like PD-1 expressed on CD8+ T cells, contribute to this functional suppression during chronic infection. However, during the acute phase of infection virus-specific CD8+ T cells express high levels of PD-1 but are fully competent in killing virus-infected cells and there is increasing evidence that the biological activity of inhibitory receptors is strongly influenced by the availability of their respective ligands. We determined the expression of ligands for inhibitory receptors on infected myeloid cells during the acute phase of Friend retroviral (FV) infection. FV infection of granulocytes, monocytes, and macrophages strongly increased the cell surface expression of PD-L1 and the recently described ligand HVEM for inhibitory receptors BTLA and CD160. In addition, the infection of human myeloid cells in vitro with HIV also enhanced the expression of PD-L1 and HVEM. In infected mice, the upregulation of inhibitory ligands on infected cells was accompanied by enhanced frequencies of FV-specific CD8+ T cells that express PD-1, and the inhibitory receptors CD160 and BTLA. To define the functional effects of HVEM on activated CD8+ T cells, FV-infected mice were treated with blocking antibodies that prevented the interaction of HVEM with its two receptors, CD160 or BTLA, alone or in combination with anti-PD-L1 antibodies. Blocking the interaction of HVEM with CD160 and BTLA improved the production of cytotoxic molecules and the elimination of FV-infected cells. This effect was augmented when the therapy was combined with anti-PD-L1 antibodies, resulting in an additional expansion of cytotoxic CD8+ T cells. Thus, the ligand HVEM for the inhibitory receptors CD160 and BTLA downregulates the functionality of CD8+ T cells during retroviral infection and are potential targets for the immunomodulatory therapy of chronic viral infections.
TNF superfamily member 14 drives post-influenza depletion of alveolar macrophages, enabling secondary pneumococcal pneumonia C Malainou, C Peteranderl, MR Ferrero, AI Vazquez-Armendariz, ... The Journal of Clinical Investigation 136 (2) , 2026 2026 Citations: 3
Infectious bursal disease virus (IBDV) as a novel oncolytic virotherapy in glioblastoma V Tur-Planells, Y Bykov, G Dawodu, N García-Romero, S Izpura-Luis, ... Journal for Immunotherapy of Cancer 13 (11), e011741 , 2025 2025 Citations: 1
A bioactive soluble recombinant mouse LIGHT promotes effective tumor immune cell infiltration delaying tumor growth ML Del Rio, OM Nuero-Garcia, G Roncador, R Garcimartín-Bailon, ... Journal of Molecular Medicine 103 (7), 867-883 , 2025 2025 Citations: 1
A sensitive functional cell-based bioassay for the screening of different recombinant variants of soluble mouse LIGHT to be used in in vivo anti-tumor studies JI Rodriguez-Barbosa, P Schneider, ML del Rio EUROPEAN JOURNAL OF IMMUNOLOGY 54, 945-945 , 2024 2024
Assesment of Hypoderma infestation in a wild population of Cervus elaphus from mountains Atlantic ecosystems in southwestern Europe (Spain) S González, R Panadero, ML Del Rio, MN Díez, M del Rosario Hidalgo, ... Veterinary Research Communications 48 (2), 761-771 , 2024 2024 Citations: 1
Contribution to the Knowledge of Gastrointestinal Nematodes in Roe Deer ( Capreolus capreolus ) from the Province of León, Spain: An Epidemiological and … S González, ML Del Rio, N Díez-Baños, A Martínez, MR Hidalgo Animals 13 (19), 3117 , 2023 2023 Citations: 8
Image_2_Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1-stem cell-like T cells over PD-1+ T cells curbing tumor progression. jpeg ML Río, C Yago-Diez de Juan, G Roncador, E Caleiras, ... Río, María Luisa del; Yago-Diez de Juan, Carla; Roncador, Giovanna; Caleiras … , 2023 2023
Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1 - stem cell-like T cells over PD-1 + T cells curbing tumor progression ML Del Rio, CYD de Juan, G Roncador, E Caleiras, R Álvarez-Esteban, ... Frontiers in Immunology 14, 1113858 , 2023 2023 Citations: 4
Identification of Hypoderma actaeon (Diptera: Oestridae) in red deer ( Cervus elaphus ) from northern Spain: Microscopy study and molecular analysis S González, ML Del Rio, MN Diez, MR Hidalgo, A Martínez Microscopy Research and Technique 86 (1), 3-11 , 2023 2023 Citations: 8
Image_1_Differential Engraftment of Parental A20 PD-L1 WT and PD-L1 KO Leukemia Cells in Semiallogeneic Recipients in the Context of PD-L1/PD-1 Interaction and NK Cell-Mediated … ML Río, JA Pérez-Simón, JI Rodríguez-Barbosa Figshare , 2022 2022
Differential engraftment of parental A20 PD-L1 WT and PD-L1 KO leukemia cells in semiallogeneic recipients in the context of PD-L1/PD-1 interaction and NK cell-mediated hybrid … ML Del Rio, JA Perez-Simon, JI Rodriguez-Barbosa Frontiers in Immunology 13, 887348 , 2022 2022 Citations: 3
The Role of the Inhibitory Ligand HVEM and Its Receptors CD160 and BTLA in the Regulation of Anti-retroviral T Cell Responses P David, J Westmeier, M Drabczyk-Pluta, T Werner, J Ickler, S Francois, ... Frontiers in Virology 2, 836291 , 2022 2022 Citations: 2
The impact of CD160 deficiency on alloreactive CD8 T cell responses and allograft rejection ML Del Rio, TH Nguyen, L Tesson, JM Heslan, A Gutierrez-Adan, ... Translational Research 239, 103-123 , 2022 2022 Citations: 8
Calidad de vida del receptor de un órgano sólido en Castilla y León VV Barrio, CF Renedo, EG Palacios, ML del Río González, MPM Sánchez, ... Revista ROL de enfermería 44 (5), 12-20 , 2021 2021 Citations: 2
Critical role of PD-L1 expression on non-tumor cells rather than on tumor cells for effective anti-PD-L1 immunotherapy in a transplantable mouse hematopoietic tumor model JI Rodriguez-Barbosa, M Azuma, G Zelinskyy, JA Perez-Simon, ... Cancer Immunology, Immunotherapy 69 (6), 1001-1014 , 2020 2020 Citations: 17
The role of TNFR2 and DR3 in the in vivo expansion of tregs in T cell depleting transplantation regimens JI Rodriguez-Barbosa, P Schneider, L Graca, L Bühler, JA Perez-Simon, ... International journal of molecular sciences 21 (9), 3347 , 2020 2020 Citations: 16
CD160 serves as a negative regulator of NKT cells in acute hepatic injury TJ Kim, G Park, J Kim, SA Lim, J Kim, K Im, MH Shin, YX Fu, ML Del Rio, ... Nature communications 10 (1), 3258 , 2019 2019 Citations: 38
HVEM, a cosignaling molecular switch, and its interactions with BTLA, CD160 and LIGHT JI Rodriguez-Barbosa, P Schneider, A Weigert, KM Lee, TJ Kim, ... Cellular & molecular immunology 16 (7), 679-682 , 2019 2019 Citations: 79
CRISPR/CAS9 gene inactivation of PD-L1 on hematopoietic tumor cells does not enhance anti-tumor immunity JI Rodríguez-Barbosa, M Ferreras, JA Pérez Simón, ML Del Río Sociedad Española de Inmunología , 2019 2019
Correction to: T follicular helper expansion and humoral-mediated rejection are independent of the HVEM/BTLA pathway JI Rodriguez-Barbosa, C Fernandez-Renedo, AMB Moral, L Bühler, ... Cellular & molecular immunology 16 (3), 314-314 , 2019 2019
MOST CITED SCHOLAR PUBLICATIONS
CD103 − and CD103 + Bronchial Lymph Node Dendritic Cells Are Specialized in Presenting and Cross-Presenting Innocuous Antigen to CD4 + and CD8 + T Cells ML del Rio, JI Rodriguez-Barbosa, E Kremmer, R Förster The Journal of immunology 178 (11), 6861-6866 , 2007 2007 Citations: 422
Development and functional specialization of CD103 + dendritic cells ML Del Rio, G Bernhardt, JI Rodriguez‐Barbosa, R Förster Immunological reviews 234 (1), 268-281 , 2010 2010 Citations: 372
Induction of tolerance to innocuous inhaled antigen relies on a CCR7-dependent dendritic cell-mediated antigen transport to the bronchial lymph node G Hintzen, L Ohl, ML del Rio, JI Rodriguez-Barbosa, O Pabst, JR Kocks, ... The Journal of Immunology 177 (10), 7346-7354 , 2006 2006 Citations: 281
HVEM/LIGHT/BTLA/CD160 cosignaling pathways as targets for immune regulation ML Del Rio, CL Lucas, L Buhler, G Rayat, JI Rodriguez-Barbosa Journal of leukocyte biology 87 (2), 223-235 , 2010 2010 Citations: 213
Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis ML Del Río, CB Gutiérrez, EF Rodríguez Ferri Journal of Clinical Microbiology 41 (2), 880-882 , 2003 2003 Citations: 138
Antibody‐mediated signaling through PD‐1 costimulates T cells and enhances CD28‐dependent proliferation ML del Rio, G Penuelas‐Rivas, R Dominguez‐Perles, P Ramirez, ... European journal of immunology 35 (12), 3545-3560 , 2005 2005 Citations: 87
HVEM, a cosignaling molecular switch, and its interactions with BTLA, CD160 and LIGHT JI Rodriguez-Barbosa, P Schneider, A Weigert, KM Lee, TJ Kim, ... Cellular & molecular immunology 16 (7), 679-682 , 2019 2019 Citations: 79
PD‐1/PD‐L1, PD‐1/PD‐L2, and other co‐inhibitory signaling pathways in transplantation ML Del Rio, L Buhler, C Gibbons, J Tian, JI Rodriguez‐Barbosa Transplant International 21 (11), 1015-1028 , 2008 2008 Citations: 76
CX 3 CR1 + c-kit + Bone Marrow Cells Give Rise to CD103 + and CD103 − Dendritic Cells with Distinct Functional Properties ML del Rio, JI Rodriguez-Barbosa, J Bölter, M Ballmaier, ... The Journal of Immunology 181 (9), 6178-6188 , 2008 2008 Citations: 68
Detection of protein on BTLAlow cells and in vivo antibody-mediated down-modulation of BTLA on lymphoid and myeloid cells of C57BL/6 and BALB/c BTLA allelic variants ML del Rio, J Kaye, JI Rodriguez-Barbosa Immunobiology 215 (7), 570-578 , 2010 2010 Citations: 51
aroA gene PCR-RFLP diversity patterns in Haemophilus parasuis and Actinobacillus species ML Del Rio, CBG Martín, J Navas, B Gutierrez-Muniz, ... Research in veterinary science 80 (1), 55-61 , 2006 2006 Citations: 46
Downregulation of BTLA on NKT cells promotes tumor immune control in a mouse model of mammary carcinoma D Sekar, L Govene, ML Del Río, E Sirait-Fischer, AF Fink, B Brüne, ... International journal of molecular sciences 19 (3), 752 , 2018 2018 Citations: 45
Efficacy of different commercial and new inactivated vaccines against ovine enzootic abortion JNG de la Fuente, CB Gutiérrez-Martın, N Ortega, EF Rodrı́guez-Ferri, ... Veterinary microbiology 100 (1-2), 65-76 , 2004 2004 Citations: 44
Identification and characterization of the TonB region and its role in transferrin-mediated iron acquisition in Haemophilus parasuis ML del Río, CB Gutiérrez-Martín, JI Rodríguez-Barbosa, J Navas, ... FEMS Immunology & Medical Microbiology 45 (1), 75-86 , 2005 2005 Citations: 43
LIGHT/HVEM/LTβR interaction as a target for the modulation of the allogeneic immune response in transplantation ML del Rio, P Schneider, C Fernandez-Renedo, JA Perez-Simon, ... American Journal of Transplantation 13 (3), 541-551 , 2013 2013 Citations: 39
CD160 serves as a negative regulator of NKT cells in acute hepatic injury TJ Kim, G Park, J Kim, SA Lim, J Kim, K Im, MH Shin, YX Fu, ML Del Rio, ... Nature communications 10 (1), 3258 , 2019 2019 Citations: 38
Molecular characterization of Haemophilus parasuis ferric hydroxamate uptake (fhu) genes and constitutive expression of the FhuA receptor ML del Río, J Navas, A Martín, C Gutiérrez, JI Rodríguez-Barbosa, ... Veterinary research 37 (1), 49-59 , 2006 2006 Citations: 36
Selective blockade of herpesvirus entry mediator–B and T lymphocyte attenuator pathway ameliorates acute graft-versus-host reaction ML del Rio, ND Jones, L Buhler, P Norris, Y Shintani, CF Ware, ... The Journal of Immunology 188 (10), 4885-4896 , 2012 2012 Citations: 35
Evaluation of survival of Actinobacillus pleuropneumoniae and Haemophilus parasuis in four liquid media and two swab specimen transport systems ML del Río, B Gutiérrez, CB Gutiérrez, JL Monter, EFR Ferri American journal of veterinary research 64 (9), 1176-1180 , 2003 2003 Citations: 22
Coating treatment in postharvest behaviour of oranges JM Martinez, J Cuquerella, MD Rio, M Mateos, RM Ded Proceedings of the Conference of Technical Innovations in Freezing and … , 1991 1991 Citations: 21
