Staphylococcus aureus’s golden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes Ahmed M. Nosair, Amal M. Abo-Kamar, Lamiaa A. Al-Madboly, Mahmoud H. Farghali, Ahmed A. Abdelaziz Microbial Cell Factories, 2026 Staphyloxanthin (STX), an apocarotenoid golden yellow pigment produced by Staphylococcus aureus isolates, is endowed with potent antioxidant capacity. It plays a crucial role in combating reactive oxygen species (ROS), exhibiting considerable application prospects. This review provides a comprehensive overview of the biosynthetic pathway and various approaches for STX bioproduction in S. aureus . Moreover, this review focuses on advancements in biotechnology for enhancing the yield of STX in S. aureus , including mutagenesis breeding, metabolic engineering, transcriptional regulation, and the optimization of fermentation conditions. This review addresses the extraction process, purification, and analytical characterization of STX pigment. Additionally, this review highlights the diverse applications of STX in healthcare settings as an antibacterial, antifungal, antiparasitic, antioxidant, anticancer, and DNA damage protection agent. To the best of our knowledge, this is the first review reporting the biotechnological aspects of STX from S. aureus as a natural biotechnologically valued product.
Investigating the potential antibacterial, anti-biofilm, wound healing and anti-inflammatory activity of the extract of Aspergillus niger endophyte isolated from cucumber leaves: in vitro and in vivo study Maisra M. El-Bouseary, Duaa Eliwa, Mahmoud H. Farghali, Amany E. Ragab BMC Microbiology, 2025 Background Endophytic fungi are a vast inventory of bioactive compounds, offering potent, cost-effective, renewable, and low-toxicity alternatives for therapeutic applications. The current investigation focused on the endophytic fungus Aspergillus niger, which was isolated for the first time from Cucumis sativus (cucumber) leaves and subjected to comprehensive evaluation, including anti-inflammatory, antibacterial, anti-biofilm, and in vitro wound healing potential. 18 S rRNA gene sequencing was utilized to identify A. niger after isolation, and the fungus was cultivated on Asian rice to produce fungal metabolites. The high-resolution liquid chromatography-mass spectrometry (LC-HRESI-MS/MS) was then used to elucidate its phytochemical profile. Results Fingerprint compounds detected in the ethyl acetate of the endophyte A. niger (ANM) revealed 15 compounds that are mainly pyrones and quinones in nature, including citric acid, nigerasperone A, aspernigrin A, aspinonene, campyrone B, aurasperone F, and plastoquinone-3. The ANM showed a strong antibacterial activity against S. aureus clinical isolates (MIC values ranging from 32 to 512 µg/mL) and a significant reduction in biofilm formation, where the total number of biofilm producers, S. aureus isolates, decreased from 19 to 6 after treatment with ½ MIC of ANM. Furthermore, ANM-treated WI38 human fibroblast cells displayed a wound closure percentage of 99.68% ± 0.02 compared to 83.37% ± 0.05 for the control cells. Additionally, the ANM demonstrated potential in promoting wound healing, particularly in infected wounds, through its antimicrobial, anti-inflammatory, and tissue-regenerating properties. Conclusions These findings highlight A. niger as a valuable source of natural therapeutics. Additional research is needed to explore its key active components and potential side effects.
Advances in biomanufacturing and recent biological applications of the fundamental bacterial carotenoids: a comprehensive review Khaled B. Al-Monofy, Mahmoud H. Farghali, Lamiaa A. Al-Madboly, Amal M. Abo Kamer, Ahmed A. Abdelaziz Microbial Cell Factories, 2025 Many research teams have prioritized the investigation of the biotechnological production of carotenoids in the last few decades due to their multipurpose application. In this review, the bioproduction of the fundamental bacterial carotenoids, including lycopene, β-carotene, β-cryptoxanthin, zeaxanthin, canthaxanthin, and astaxanthin, is discussed, along with detailed information on their biosynthesis, laboratory screening, physiochemical requirements, genetic engineering-based production development, and fermentation techniques. As well, this review emphasizes that the bacterial carotenoids are innovative therapeutic avenues with diverse biological applications, from food additives to anticancer agents. Moreover, the review discusses the limitations of the application of these carotenoids and the future perspectives. Ultimately, the review highlights the bacteria as powerful cell factories, potentially facilitating the commercial production of carotenoids in the future.
Nutritional optimization for bioprocess production of staphyloxanthin from Staphylococcus aureus with response surface methodology: promising anticancer scaffold targeting EGFR inhibition Ahmed M. Nosair, Ahmed A. Abdelaziz, Amal M. Abo-Kamer, Lamiaa A. Al-Madboly, Mahmoud H. Farghali Microbial Cell Factories, 2025 Background Staphyloxanthin (STX) is a secondary metabolite pigment associated with membrane structures, recognized for its significant antioxidant properties. It plays a crucial role in combating reactive oxygen species (ROS), positioning it as a promising and effective alternative in cancer treatment. This study focused on enhancing the production of STX pigment by employing statistical optimization of media components, alongside the evaluation of its safety and anticancer properties. Results A total of 59 Staphylococcus aureus isolates were screened and quantitatively estimated for STX production. The best pigment-producing isolate was identified based on molecular phylogenetic analysis as S. aureus A2, with accession number PP197164. A Box-Wilson central composite design was employed to evaluate the intricate interactions among six variables affecting the pigment yield. The most optimal conditions resulted in the highest production of STX of OD456 = 0.328, which is approximately 1.5-fold greater than the yield (OD456 = 0.215) obtained from OFAT optimization. The final response surface model fitting the data achieved a R² of 0.8748. STX exhibited marked cytotoxicity against the A549 NSCLC cell line with IC50 of 57.3 µg/mL, a safe dose in normal Vero cells. The anticancer activity of STX was predominantly mediated by the apoptotic pathway, as confirmed by confocal microscopy, the annexin V-FITC apoptosis assay, and the overexpression of caspase-3. Moreover, STX disrupted cell cycle at pre-G1 and G0/G1 phases in lung cancer. Intriguingly, STX exhibited its antitumor activity through reducing the EGFR expression. The molecular docking study revealed the potential binding interactions and affinities within the active sites of both wild-type and mutant EGFR. Conclusion The bioprocess for optimized production, combined with the biological profiling and low cytotoxicity, substantiates the potential application of STX pigment in combating lung cancer.
Coating silicon catheters with the optimized and stable carotenoid bioproduct from Micrococcus luteus inhibited the biofilm formation by multidrug-resistant Enterococcus faecalis via downregulation of GelE gene expression Khaled B. Al-Monofy, Ahmed A. Abdelaziz, Amal M. Abo-Kamar, Lamiaa A. Al-Madboly, Mahmoud H. Farghali Microbial Cell Factories, 2025 Background Microbial carotenoids have gained industry interest due to their safety and diverse biological activities; however, the low yield of carotenoids hinders their applications. Hence, this study focused on optimizing carotenoid pigment production from Micrococcus luteus strains by studying 54 physical and chemical independent conditions. The chronic infections by Enterococcus faecalis are related to its ability to form biofilms on the surface of several implanted medical devices, such as urinary catheters. Therefore, the potential antibacterial and antibiofilm activities of the purified pigment against E. faecalis were investigated in our study. Results Using one-factor-at-a-time experiments, the top-examined parameters were tryptic soya broth (TSB), agitation, temperature, pH, incubation time, inoculum size (IS), sodium chloride, tryptophan, glycerol, tryptone, glutaric acid, toluene, ferric sulphate, and disodium hydrogen phosphate. The data from the Plackett-Burman design showed that temperature, sodium chloride, tryptophan, and toluene were fundamental factors controlling carotenoid production. The conditions for the upstream process were determined via response surface methodology design, which included TSB medium, agitation speed of 120 rpm, temperature of 32.5 °C, pH = 7, incubation time of 96 h, 2% IS, sodium chloride (12.5 g/l), tryptophan (12.5 g/l), glutaric acid (5 g/l), toluene (12.5%), and disodium hydrogen phosphate (5 g/l). Submerged fermentation model validation using the M6 isolate (accession number of PP197163) revealed an increase in carotenoid production up to 6-fold (1.2 g/l). The produced pigment was purified and characterized as β-carotene, and the stability study showed that the extracted β-carotene was stable for a year in dimethyl sulfoxide at 4 °C. The MTT test data proved that the pigment was safe on human dermal fibroblasts with an IC50 equal to 542.7 µg/ml. For the first time, it was reported that the stable purified β-carotene exhibited powerful antibacterial activity against multidrug-resistant (MDR) E. faecalis, with inhibition zones ranging from 13 to 32 mm and minimum inhibitory concentrations (MICs) ranging from 3.75 to 30 µg/ml at safe concentrations. In addition, it was found that our stable purified β-carotene showed up to 94% inhibition in biofilm formation by strong biofilm-forming E. faecalis. In addition, the β-carotene-coated catheter manifested a lower biofilm formation by E. faecalis by up to 75.3%. Moreover, crystal violet staining, dual staining, and fluorescence staining techniques displayed immature biofilms of E. faecalis when treated with 0.25 and 0.5 MICs of β-carotene. The mechanistic pathway for the purified β-carotene’s antibiofilm activity was strongly linked to the inhibition of gelatinase enzyme production (up to 100% inhibition) as manifested phenotypically, genotypically, and by molecular docking. Conclusion This work provided a deeper insight into optimizing carotenoid production from M. luteus by investigating the influence of 54 diverse conditions. Also, this is the first time to report the antibacterial and antibiofilm actions of the stable purified microbial β-carotene against strong biofilm-forming MDR E. faecalis colonizing urinary catheters.
Deciphering the efficacy of staphyloxanthin-encapsulated niosomal nanovesicles to attenuate biofilm formation, quorum sensing, and meropenem persistence in Acinetobacter baumannii Ahmed M. Nosair, Ahmed A. Abdelaziz, Amal M. Abo-Kamar, Lamiaa A. Al-Madboly, Mahmoud H. Farghali BMC Microbiology, 2025 Background Acinetobacter baumannii is the primary cause of persistent opportunistic infections in healthcare settings, recognized as a global priority due to its resistance to antibiotic therapy. Quorum sensing and biofilm formation are the key factors driving the pathogenesis and drug resistance of A. baumannii . Nanostructures demonstrated encouraging promise in enhancing the therapeutic efficacy and overcoming treatment failure. Therefore, the efficacy of staphyloxanthin (STX)-encapsulated niosomes was evaluated both in vitro and in vivo. Results The formulated niosomal nanovesicles displayed a spherical shape at the nanoscale (177.8 nm), featuring a slow-release rate (39.6%) and appropriate entrapment efficiency (92.7%). Our results demonstrated that STX exhibited strong antibacterial activity, with MIC values up to 16 µg/mL against multidrug-resistant isolates ( n = 24). The in vitro findings revealed that the encapsulation of STX within niosomal nanovesicles demonstrated superior therapeutic efficacy compared to the free solution. This improvement was reflected by a significant reduction in biofilm formation (68–88%), motility (66.66–94.45%), and siderophore production (48.75–79.5%), as well as marked disruption of the mature biofilm by 82%. The anti-quorum sensing activity of STX was further confirmed the attenuation of biofilm and virulence, as evidenced by downregulation of abaI expression (1.42-fold reduction) and molecular docking simulations. It is noteworthy that the biological findings revealed a significant eradication of meropenem-induced persister cells after the addition of niosomal dispersion. The preclinical investigations prove the efficacy of STX in improving survival rates through reducing the bacterial burden (2-fold reduction) and lethal inflammatory consequences in a mouse model of pneumonia. Conclusion our results suggested that STX may serve as a promising alternative for combating A. baumannii biofilms and persister cells.
The dual antifungal and antibiofilm activities of β-carotene against multidrug-resistant Candida albicans induce wound healing in a diabetic rat model: an in vitro and in vivo study Khaled B. Al-Monofy, Ahmed A. Abdelaziz, Amal M. Abo-Kamar, Lamiaa A. Al-Madboly, Mahmoud H. Farghali BMC Microbiology, 2025 Background Diabetes mellitus renders patients susceptible to chronic wounds and fungal infections, such as Candida albicans infections. The treatment of C. albicans -infected diabetic wounds is often problematic due to drug resistance and biofilm formation. Therefore, we studied the impact of β-carotene on treating diabetic wounds infected with strong biofilm-forming multidrug-resistant (MDR) C. albicans . Results Our data showed that β-carotene (200 µg/ml) exhibited potent antifungal activity against the planktonic C. albicans cells, including MDR cells, with inhibition zones of 14 to 34 mm, which was emphasized by methylene blue staining and growth kinetics analysis. Using polystyrene plates and silicon catheter models, β-carotene showed a promising antibiofilm action by disrupting the established biofilms of C. albicans by 95% and 75.9%, respectively, which was confirmed by crystal violet staining, dual staining, and fluorescence staining approaches. The antibiofilm pathway of β-carotene was also examined, and it was found that β-carotene targeted the first stage of biofilm via reducing the adherence up to 90%, which was linked to downregulation of expression of the hypha-specific gene, ALS3 , and blocking action of agglutinin-like protein 3 (Als3) via complexation (-9.8 kcal/mol). In addition, β-carotene disrupted the initiation step by inhibiting the yeast-to-hyphae transition and lowering the viability of sessile cells up to 80%. Furthermore, the maturation step of biofilm was embattled by β-carotene through a 95.6% reduction in the production of polysaccharide matrix. The in vivo model demonstrated the curative role of β-carotene in the healing process, which displayed a wound healing ratio of 89%, a lower fungal burden, and better histological features. Conclusion Our findings demonstrated the therapeutic potential of β-carotene for treating diabetic wounds infected with strong biofilm-forming MDR C. albicans .
Investigation of antibacterial and wound healing activities of the extract of Rhodotorula mucilaginosa endophyte isolated from cucumber leaves Duaa Eliwa, Maisra M. El-Bouseary, Mahmoud H. Farghali, Thanaa A. El-Masry, Amany E. Ragab Scientific Reports, 2025 Endophytic fungi represent a reservoir of pharmacologically essential secondary metabolites. The current study focused on the antibacterial properties of the endophytic yeast-like fungus Rhodotorula mucilaginosa (R. mucilaginosa) isolated for the first time from Cucumis sativus (cucumber) leaves. After isolation, R. mucilaginosa was identified by 18S rRNA gene sequencing and was cultured on Asian rice for production of fungal metabolites. Then, its phytochemical profile was elucidated using LC–HRESI–MS/MS technique to reveal 22 compounds which are mainly carotenoids and fatty acids in nature. Dried ethyl acetate extract of R. mucilaginosa was tested for antimicrobial activity against Pseudomonas aeruginosa isolates. The extract of R. mucilaginosa (ERM) showed minimum inhibitory concentrations with a range from 64 to 512 µg/mL. The crystal violet assay was utilized to determine the effect of ERM on the tested isolates’ ability to produce biofilms. The percentage of strong biofilm-forming isolates dropped from 20 to 3.3% and 10%, respectively, after the treatment with ½ and ¼ MICs of ERM. When tested isolates of P. aeruginosa were exposed to sub-MICs of ERM, their cell size significantly decreased, and their biofilm matrix was reduced. The in vitro anti-inflammatory activity of ERM was supported by the marked decrease in TNF-α gene expression in the cells treated with either ERM or piroxicam compared to the control cells. The wound healing effect of ERM was investigated in vitro, where ERM showed improvement in the wound healing process. In addition, the wound healing effect of ERM was investigated in vivo using a model of infection in rats, where the wounds were infected with P. aeruginosa. The histological investigation of the wound showed a remarkable improvement in the group treated with ERM. Therefore, further investigation is required to explore the possible application of ERM as a potential antibacterial agent, which could aid in our fight against pathogenic microbes that have become resistant.
Staphyloxanthin loaded niosomal nanocarrier augments its anthelmintic activity against Trichinella spiralis infection in mice Ahmed M. Nosair, Ahmed A. Abdelaziz, Amal M. Abo-Kamar, Hager S. Zoghroban, Mahmoud H. Farghali, et al. Scientific Reports, 2025 Trichinellosis, a resurgent zoonotic infestation, threatens public health due to recorded human outbreaks in various nations. The emergence of treatment resistance necessitates the exploration of efficient natural alternatives. Staphyloxanthin (STX), a membrane-associated secondary metabolite carotenoid pigment, underscores pro-oxidative traits, positioning it as a novel therapeutic candidate. Nanostructures demonstrated encouraging promise in overcoming low oral bioavailability, which could undermine the efficacy. Hence, the therapeutic outcome of STX-loaded niosomes was scrutinized both in vitro and in vivo. In this study, the prepared niosomal nanovesicles exhibited a spherical form in the nanoscale spectrum. Our in vitro findings demonstrated that STX markedly diminished larval viability, associated with excessive cuticular deformities, numerous notches, and membrane blebbing. The preclinical evaluation revealed that the oral delivery of STX-niosomes showed a superiority of therapeutic efficacy in mice compared to the reference drug. This was reflected by the eradicated adult worms, enhanced histopathological attributes, and reduced larval count. It is noteworthy that the biological findings revealed a significant reduction in the inflammatory expression of TNF-α surrounding trichina capsules. The relationship between STX and the parasite was elucidated, with the promising antiparasitic efficacy being further corroborated through in silico homology modelling and molecular docking approaches. The 3D-modelled target protein structures exhibited excellent quality factors and favourable Ramachandran plot statistics. Intriguingly, in silico docking results obviously revealed the potential affinity of STX to bind and block target protein receptors. In conclusion, our results suggested that STX pigment may serve as a promising pioneering alternative in the anthelmintic fight against trichinellosis.
Staphylococcus aureus’ sgolden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes AM Nosair, AM Abo-Kamar, LA Al-Madboly, MH Farghali, AA Abdelaziz Microbial Cell Factories , 2026 2026 Citations: 1
Deciphering the efficacy of staphyloxanthin-encapsulated niosomal nanovesicles to attenuate biofilm formation, quorum sensing, and meropenem persistence in Acinetobacter baumannii AM Nosair, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, MH Farghali BMC microbiology 25 (1), 791 , 2025 2025 Citations: 1
The dual antifungal and antibiofilm activities of β-carotene against multidrug-resistant Candida albicans induce wound healing in a diabetic rat model: an in vitro and in vivo … KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... BMC microbiology 25 (1), 747 , 2025 2025 Citations: 2
Advances in biomanufacturing and recent biological applications of the fundamental bacterial carotenoids: a comprehensive review KB Al-Monofy, MH Farghali, LA Al-Madboly, AMA Kamer, AA Abdelaziz Microbial Cell Factories 24 (1), 225 , 2025 2025 Citations: 2
Staphyloxanthin loaded niosomal nanocarrier augments its anthelmintic activity against Trichinella spiralis infection in mice AM Nosair, AA Abdelaziz, AM Abo-Kamar, HS Zoghroban, MH Farghali, ... Scientific reports 15 (1), 32446 , 2025 2025 Citations: 3
Investigation of antibacterial and wound healing activities of the extract of Rhodotorula mucilaginosa endophyte isolated from cucumber leaves D Eliwa, MM El-Bouseary, MH Farghali, TA El-Masry, AE Ragab Scientific Reports 15 (1), 31730 , 2025 2025 Citations: 2
Coating silicon catheters with the optimized and stable carotenoid bioproduct from Micrococcus luteus inhibited the biofilm formation by multidrug-resistant Enterococcus … KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... Microbial Cell Factories 24 (1), 186 , 2025 2025 Citations: 2
In vitro and in vivo investigation of the antibacterial, antivirulence, and antiquorum sensing activities of β-carotene against difficult-to-treat resistant Pseudomonas aeruginosa KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... BMC microbiology 25 (1), 501 , 2025 2025 Citations: 6
Potential Antibacterial, Wound Healing and Anti-inflammatory Activities of Penicillium rubens, an Endophytic Fungus Isolated from the Leaves of Cucumis sativus L. M Farghali, D Eliwa, MM El-Bouseary Frontiers in Scientific Research and Technology 11 (1) , 2025 2025 Citations: 2
Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital D Yousef, AM Soliman, F Sonbol, AM Abo Kamer, H Ramadan, ... Microbes and Infectious Diseases 6 (3), 4020-4034 , 2025 2025
Investigating the potential antibacterial, anti-biofilm, wound healing and anti-inflammatory activity of the extract of Aspergillus niger endophyte isolated from … MM El-Bouseary, D Eliwa, MH Farghali, AE Ragab BMC microbiology 25 (1), 420 , 2025 2025 Citations: 3
Nutritional optimization for bioprocess production of staphyloxanthin from Staphylococcus aureus with response surface methodology: promising anticancer scaffold targeting EGFR … LAAMMHF Ahmed M. Nosair, Ahmed A. Abdelaziz, Amal M. Abo-Kamer Microbial cell factories , 2025 2025 Citations: 10
Khaled B. Al-Monofy1, Ahmed A. Abdelaziz1, Amal M. Abo-Kamar1, Lamiaa A. Al-Madboly1 and MH Farghali 2025
Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital SA Alshaikh, T El-Banna, F Sonbol, MH Farghali Annals of Clinical Microbiology and Antimicrobials 23 (1), 20 , 2024 2024 Citations: 64
In-vitro investigation of biofilm-specific resistance and virulence of biofilm-forming uropathogenic Escherichia coli S A Alshaikh, T El-Banna, F Sonbol, M H Farghali Novel Research in Microbiology Journal 8 (1), 2285-2302 , 2024 2024
SARS-CoV-2 in Egypt: epidemiology, clinical characterization and bioinformatics analysis B Alotaibi, TA El-Masry, MG Seadawy, MH Farghali, BE El-Harty, A Saleh, ... Heliyon 8 (2) , 2022 2022 Citations: 5
Effects of Moloney leukemia virus 10 protein on hepatitis B virus infection and viral replication MN Puray-Chavez, MH Farghali, V Yapo, AD Huber, D Liu, TP Ndongwe, ... Viruses 11 (7), 651 , 2019 2019 Citations: 13
Modulation of HBV infection and replication by cell-derived factors MHM Farghali University of Missouri--Columbia , 2019 2019
Biocide resistance and its impact on antibiotic susceptibility in Pseudomonas aeruginosa M Farghali Faculty of Pharmacy, Tanta University , 2013 2013
MOST CITED SCHOLAR PUBLICATIONS
Correlation between antimicrobial resistance, biofilm formation, and virulence determinants in uropathogenic Escherichia coli from Egyptian hospital SA Alshaikh, T El-Banna, F Sonbol, MH Farghali Annals of Clinical Microbiology and Antimicrobials 23 (1), 20 , 2024 2024 Citations: 64
Effects of Moloney leukemia virus 10 protein on hepatitis B virus infection and viral replication MN Puray-Chavez, MH Farghali, V Yapo, AD Huber, D Liu, TP Ndongwe, ... Viruses 11 (7), 651 , 2019 2019 Citations: 13
Nutritional optimization for bioprocess production of staphyloxanthin from Staphylococcus aureus with response surface methodology: promising anticancer scaffold targeting EGFR … LAAMMHF Ahmed M. Nosair, Ahmed A. Abdelaziz, Amal M. Abo-Kamer Microbial cell factories , 2025 2025 Citations: 10
In vitro and in vivo investigation of the antibacterial, antivirulence, and antiquorum sensing activities of β-carotene against difficult-to-treat resistant Pseudomonas aeruginosa KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... BMC microbiology 25 (1), 501 , 2025 2025 Citations: 6
SARS-CoV-2 in Egypt: epidemiology, clinical characterization and bioinformatics analysis B Alotaibi, TA El-Masry, MG Seadawy, MH Farghali, BE El-Harty, A Saleh, ... Heliyon 8 (2) , 2022 2022 Citations: 5
Staphyloxanthin loaded niosomal nanocarrier augments its anthelmintic activity against Trichinella spiralis infection in mice AM Nosair, AA Abdelaziz, AM Abo-Kamar, HS Zoghroban, MH Farghali, ... Scientific reports 15 (1), 32446 , 2025 2025 Citations: 3
Investigating the potential antibacterial, anti-biofilm, wound healing and anti-inflammatory activity of the extract of Aspergillus niger endophyte isolated from … MM El-Bouseary, D Eliwa, MH Farghali, AE Ragab BMC microbiology 25 (1), 420 , 2025 2025 Citations: 3
The dual antifungal and antibiofilm activities of β-carotene against multidrug-resistant Candida albicans induce wound healing in a diabetic rat model: an in vitro and in vivo … KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... BMC microbiology 25 (1), 747 , 2025 2025 Citations: 2
Advances in biomanufacturing and recent biological applications of the fundamental bacterial carotenoids: a comprehensive review KB Al-Monofy, MH Farghali, LA Al-Madboly, AMA Kamer, AA Abdelaziz Microbial Cell Factories 24 (1), 225 , 2025 2025 Citations: 2
Investigation of antibacterial and wound healing activities of the extract of Rhodotorula mucilaginosa endophyte isolated from cucumber leaves D Eliwa, MM El-Bouseary, MH Farghali, TA El-Masry, AE Ragab Scientific Reports 15 (1), 31730 , 2025 2025 Citations: 2
Coating silicon catheters with the optimized and stable carotenoid bioproduct from Micrococcus luteus inhibited the biofilm formation by multidrug-resistant Enterococcus … KB Al-Monofy, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, ... Microbial Cell Factories 24 (1), 186 , 2025 2025 Citations: 2
Potential Antibacterial, Wound Healing and Anti-inflammatory Activities of Penicillium rubens, an Endophytic Fungus Isolated from the Leaves of Cucumis sativus L. M Farghali, D Eliwa, MM El-Bouseary Frontiers in Scientific Research and Technology 11 (1) , 2025 2025 Citations: 2
Staphylococcus aureus’ sgolden-yellow pigment staphyloxanthin: production enhancement, analytical characterization, and biological attributes AM Nosair, AM Abo-Kamar, LA Al-Madboly, MH Farghali, AA Abdelaziz Microbial Cell Factories , 2026 2026 Citations: 1
Deciphering the efficacy of staphyloxanthin-encapsulated niosomal nanovesicles to attenuate biofilm formation, quorum sensing, and meropenem persistence in Acinetobacter baumannii AM Nosair, AA Abdelaziz, AM Abo-Kamar, LA Al-Madboly, MH Farghali BMC microbiology 25 (1), 791 , 2025 2025 Citations: 1
Detection of plasmid-borne mcr genes conferring colistin resistance in MDR and XDR Gram-negative bacterial isolates from an Egyptian hospital D Yousef, AM Soliman, F Sonbol, AM Abo Kamer, H Ramadan, ... Microbes and Infectious Diseases 6 (3), 4020-4034 , 2025 2025
Khaled B. Al-Monofy1, Ahmed A. Abdelaziz1, Amal M. Abo-Kamar1, Lamiaa A. Al-Madboly1 and MH Farghali 2025
In-vitro investigation of biofilm-specific resistance and virulence of biofilm-forming uropathogenic Escherichia coli S A Alshaikh, T El-Banna, F Sonbol, M H Farghali Novel Research in Microbiology Journal 8 (1), 2285-2302 , 2024 2024
Modulation of HBV infection and replication by cell-derived factors MHM Farghali University of Missouri--Columbia , 2019 2019
Biocide resistance and its impact on antibiotic susceptibility in Pseudomonas aeruginosa M Farghali Faculty of Pharmacy, Tanta University , 2013 2013
