Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus Maíra N. de Almeida, Valéria M. Guimarães, Daniel L. Falkoski, Brenda R. de Camargo, Gizele C. Fontes-Sant'ana, et al. Journal of Food Biochemistry, 2018 Two intracellular invertases from Aspergillus terreus were purified and characterized. Invertase 1 has been shown maximal activity at pH 3.0 and optimum temperature at 60°C, while invertase 2 presented maximal activity at pH 4.6 and optimum temperature at 55°C. Kₘ of invertase 1 and 2 for sucrose were 9.8 and 6.2 mM, respectively. Invertase 2 showed high thermostability presenting half‐life of 44, 14.9, and 2 hr at 40, 55, and 60°C, respectively. Gel filtration and SDS‐PAGE analysis revealed that invertase 2 has a dimeric structure composed by two monomers of 32 kDa. Fructose was a competitive inhibitor for invertase 2 presenting Kᵢ of 61.5 mM. Besides hydrolytic activity, invertase 2 also showed transfructosylating activity producing fructooligosaccharides. The enzymes have potential to be applied in food industry since its product, inverted sugar, is used in candies and syrup production, while fructooligosacharides are prebiotics, low calorie and noncariogenic sweeteners. PRACTICAL APPLICATIONS: Enzymes invertases hydrolyze sucrose in glucose and fructose producing inverted sugar. This product is widely used in food industry to produce jams, syrups, and fondant fillings for chocolates. Besides hydrolysis activity, some invertases can also present transfructosylating activity producing fructooligosaccharides. These oligosaccharides can act as prebiotic, providing beneficial health effects and can be used as low calorie and noncariogenic sweeteners. Aspergillus terreus produced two intracellular enzymes, one exclusively invertase, and the other, invertase and transfructosylase. The enzymes were purified and characterized in this study. This is an important step on screening new enzymes able to produce inverted sugar and fructooligosaccharides from sucrose.
Biochemical Characterization and Application of Amylase from the Fungus Acremonium zeae in the Hydrolysis of Animal Feed EGR Sales, LOC Santos, SP de Oliveira, LF Costa, PS Carrê, GM Piazzi, ... Advances in Sciences Behind Food, Energy, and Innovation: Selected … , 2025 2025
Magnetic nanoparticle-immobilized enzymes for efficient raffinose-oligosaccharide hydrolysis AM de Alcântara, GM Piazzi, EGR Sales, IPP do Nascimento, ... Biocatalysis and Biotransformation 43 (4), 448-459 , 2025 2025 Citations: 1
Purification and characterization of α-galactosidases from Penicillium griseoroseum for efficient soymilk hydrolysis DL Falkoski, ST de Rezende, VM Guimarães, MV de Queiroz, ... Biochemical and Biophysical Research Communications 737, 150905 , 2024 2024 Citations: 4
Two α-Arabinofuranosidases from Chrysoporthe cubensis and Their Effects on Sugarcane Bagasse Saccharification MFG de Albuquerque, MN de Almeida, MP Tavares, ... BioEnergy Research 17 (3), 1584-1597 , 2024 2024 Citations: 5
Exploring the potential of coffee husks as a raw material for second-generation ethanol production MN de Almeida, GG Halfeld, IB da Costa, LG de Lima Guimarães, ... BioEnergy Research 17 (1), 281-293 , 2024 2024 Citations: 23
Brachiaria brizantha grass as a feedstock for ethanol production RS Rodrigues, MN Almeida, GP Maitan-Alfenas, RZ Ventorim, SR Sartori, ... Brazilian Archives of Biology and Technology 64, e21200397 , 2021 2021 Citations: 7
Production of oligosaccharides by fungi or fungal enzymes MN de Almeida, GP Maitan-Alfenas Elsevier , 2021 2021 Citations: 7
Myceliophthora thermophila Xyr1 is predominantly involved in xylan degradation and xylose catabolism AC dos Santos Gomes, D Falkoski, E Battaglia, M Peng, ... Biotechnology for biofuels 12 (1), 220 , 2019 2019 Citations: 36
Study of gamba grass as carbon source for cellulase production by Fusarium verticillioides and its application on sugarcane bagasse saccharification MN de Almeida, DL Falkoski, VM Guimarães, ST de Rezende Industrial Crops and Products 133, 33-43 , 2019 2019 Citations: 30
Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus MN de Almeida, VM Guimarães, DL Falkoski, BR de Camargo, ... Journal of Food Biochemistry 42 (5), e12551 , 2018 2018 Citations: 35
Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling EM Visser, TF Leal, MN de Almeida, VM Guimarães Biotechnology for biofuels 8 (1), 5 , 2015 2015 Citations: 81
Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase GP Maitan-Alfenas, LGA Lage, MN de Almeida, EM Visser, ... Food chemistry 146, 429-436 , 2014 2014 Citations: 50
Optimization of Endoglucanase and Xylanase Activities from Fusarium verticillioides for Simultaneous Saccharification and Fermentation of Sugarcane Bagasse MN de Almeida, VM Guimarães, DL Falkoski, GBT Paes, JI Ribeiro Jr, ... Applied biochemistry and biotechnology 172 (3), 1332-1346 , 2014 2014 Citations: 43
Direct ethanol production from glucose, xylose and sugarcane bagasse by the corn endophytic fungi Fusarium verticillioides and Acremonium zeae MN de Almeida, VM Guimarães, DL Falkoski, EM Visser, GA Siqueira, ... Journal of Biotechnology 168 (1), 71-77 , 2013 2013 Citations: 48
Production and application of an enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum with potential for hydrolysis of sugarcane bagasse EM Visser, DL Falkoski, MN de Almeida, GP Maitan-Alfenas, ... Bioresource technology 144, 587-594 , 2013 2013 Citations: 80
Characteristics of free endoglucanase and glycosidases multienzyme complex from Fusarium verticillioides MN de Almeida, DL Falkoski, VM Guimarães, HJO Ramos, EM Visser, ... Bioresource technology 143, 413-422 , 2013 2013 Citations: 45
Chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes DL Falkoski, VM Guimarães, MN de Almeida, AC Alfenas, JL Colodette, ... Bioresource technology 130, 296-305 , 2013 2013 Citations: 99
Complexo celulolítico e hemicelulolítico do fungo endofítico Fusarium verticillioides e sua aplicação para sacarificação do bagaço de cana. MN Almeida 2013 Citations: 1
Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification DL Falkoski, VM Guimarães, MN de Almeida, AC Alfenas, JL Colodette, ... Applied biochemistry and biotechnology 166 (6), 1586-1603 , 2012 2012 Citations: 67
Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis MN de Almeida, VM Guimarães, KM Bischoff, DL Falkoski, OL Pereira, ... Applied Biochemistry and Biotechnology 165 (2), 594-610 , 2011 2011 Citations: 100
MOST CITED SCHOLAR PUBLICATIONS
Cellulases and Hemicellulases from Endophytic Acremonium Species and Its Application on Sugarcane Bagasse Hydrolysis MN de Almeida, VM Guimarães, KM Bischoff, DL Falkoski, OL Pereira, ... Applied Biochemistry and Biotechnology 165 (2), 594-610 , 2011 2011 Citations: 100
Chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes DL Falkoski, VM Guimarães, MN de Almeida, AC Alfenas, JL Colodette, ... Bioresource technology 130, 296-305 , 2013 2013 Citations: 99
Increased enzymatic hydrolysis of sugarcane bagasse from enzyme recycling EM Visser, TF Leal, MN de Almeida, VM Guimarães Biotechnology for biofuels 8 (1), 5 , 2015 2015 Citations: 81
Production and application of an enzyme blend from Chrysoporthe cubensis and Penicillium pinophilum with potential for hydrolysis of sugarcane bagasse EM Visser, DL Falkoski, MN de Almeida, GP Maitan-Alfenas, ... Bioresource technology 144, 587-594 , 2013 2013 Citations: 80
Characterization of Cellulolytic Extract from Pycnoporus sanguineus PF-2 and Its Application in Biomass Saccharification DL Falkoski, VM Guimarães, MN de Almeida, AC Alfenas, JL Colodette, ... Applied biochemistry and biotechnology 166 (6), 1586-1603 , 2012 2012 Citations: 67
Hydrolysis of soybean isoflavones by Debaryomyces hansenii UFV-1 immobilised cells and free β-glucosidase GP Maitan-Alfenas, LGA Lage, MN de Almeida, EM Visser, ... Food chemistry 146, 429-436 , 2014 2014 Citations: 50
Direct ethanol production from glucose, xylose and sugarcane bagasse by the corn endophytic fungi Fusarium verticillioides and Acremonium zeae MN de Almeida, VM Guimarães, DL Falkoski, EM Visser, GA Siqueira, ... Journal of Biotechnology 168 (1), 71-77 , 2013 2013 Citations: 48
Characteristics of free endoglucanase and glycosidases multienzyme complex from Fusarium verticillioides MN de Almeida, DL Falkoski, VM Guimarães, HJO Ramos, EM Visser, ... Bioresource technology 143, 413-422 , 2013 2013 Citations: 45
Optimization of Endoglucanase and Xylanase Activities from Fusarium verticillioides for Simultaneous Saccharification and Fermentation of Sugarcane Bagasse MN de Almeida, VM Guimarães, DL Falkoski, GBT Paes, JI Ribeiro Jr, ... Applied biochemistry and biotechnology 172 (3), 1332-1346 , 2014 2014 Citations: 43
Myceliophthora thermophila Xyr1 is predominantly involved in xylan degradation and xylose catabolism AC dos Santos Gomes, D Falkoski, E Battaglia, M Peng, ... Biotechnology for biofuels 12 (1), 220 , 2019 2019 Citations: 36
Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus MN de Almeida, VM Guimarães, DL Falkoski, BR de Camargo, ... Journal of Food Biochemistry 42 (5), e12551 , 2018 2018 Citations: 35
Study of gamba grass as carbon source for cellulase production by Fusarium verticillioides and its application on sugarcane bagasse saccharification MN de Almeida, DL Falkoski, VM Guimarães, ST de Rezende Industrial Crops and Products 133, 33-43 , 2019 2019 Citations: 30
Characterization of an exoinulinase produced by Aspergillus terreus CCT 4083 grown on sugar cane bagasse JB Coitinho, VM Guimarães, MN de Almeida, DL Falkoski, JH de Queiróz, ... Journal of agricultural and food chemistry 58 (14), 8386-8391 , 2010 2010 Citations: 30
Exploring the potential of coffee husks as a raw material for second-generation ethanol production MN de Almeida, GG Halfeld, IB da Costa, LG de Lima Guimarães, ... BioEnergy Research 17 (1), 281-293 , 2024 2024 Citations: 23
Covalent Immobilization of α-Galactosidase from Penicillium griseoroseum and its Application in Oligosaccharides Hydrolysis DL Falkoski, VM Guimarães, MV de Queiroz, EF de Araújo, ... Applied biochemistry and biotechnology 158 (3), 540-551 , 2009 2009 Citations: 13
Hydrolysis of galacto-oligosaccharides in soy molasses by α-galactosidases and invertase from Aspergillus terreus AP Reis, VM Guimarães, JG Ferreira, JH Queiroz, MGA Oliveira, ... Brazilian Archives of Biology and Technology 53 (3), 719-729 , 2010 2010 Citations: 10
Brachiaria brizantha grass as a feedstock for ethanol production RS Rodrigues, MN Almeida, GP Maitan-Alfenas, RZ Ventorim, SR Sartori, ... Brazilian Archives of Biology and Technology 64, e21200397 , 2021 2021 Citations: 7
Production of oligosaccharides by fungi or fungal enzymes MN de Almeida, GP Maitan-Alfenas Elsevier , 2021 2021 Citations: 7
Two α-Arabinofuranosidases from Chrysoporthe cubensis and Their Effects on Sugarcane Bagasse Saccharification MFG de Albuquerque, MN de Almeida, MP Tavares, ... BioEnergy Research 17 (3), 1584-1597 , 2024 2024 Citations: 5
Purification and characterization of α-galactosidases from Penicillium griseoroseum for efficient soymilk hydrolysis DL Falkoski, ST de Rezende, VM Guimarães, MV de Queiroz, ... Biochemical and Biophysical Research Communications 737, 150905 , 2024 2024 Citations: 4