Hydrodynamic comparison of different geometries of square cross-section airlift bioreactor using computational fluid dynamics Mateus N. Esperança, Marcel O. Cerri, Vítor T. Mazziero, Rodrigo Béttega, Alberto C. Badino International Journal of Chemical Reactor Engineering, 2023 The hydrodynamics of airlift bioreactors, which offer an interesting alternative to conventional stirred-tank bioreactors, has generally been evaluated using experimental approaches, requiring time, energy, and reagents. However, computational fluid dynamics (CFD) has emerged as an important and valuable tool for the analysis and design of these devices, saving time and experimental effort, while providing a large amount of information. In this study, four geometries of a square cross-section 10-L split airlift bioreactor operating with distilled water were simulated using CFD, and the hydrodynamics variables gas hold-up and liquid velocity were evaluated. CFD satisfactorily predicted the hydrodynamic parameters, when compared to experimental data, allowing adequate prediction of the shear rate distribution in airlift bioreactors. The results indicated that different shear rate distributions were obtained by geometric modifications in the bioreactor, showing that its design should be considered to satisfy different specific bioprocess requirements.
Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives Renata M. M. G. P. Ribeiro, Bruno W. Picão, Daniele O. Gonçalves, Mateus Scontri, Vitor T. Mazziero, et al. Fermentation, 2023 The production of carotenoids by microbial organisms has gained significant interest due to the growing demand for natural products. Among the non-model oleaginous red yeasts, Rhodotorula toruloides stands out as an appealing host for natural carotenoid production. R. toruloides possesses the natural ability to metabolize a wide range of substrates, including lignocellulosic hydrolysates, and convert them into lipids and carotenoids. In this study, we focused on utilizing xylose, the main component of hemicellulose, as the major substrate for R. toruloides. We conducted a comprehensive kinetic evaluation to examine the impact of aeration and agitation on carotenoid production. Results in stirred-tank reactor demonstrated that under milder conditions (300 rpm and 0.5 vvm), R. toruloides accumulated over 70% of its cell mass as lipids. Furthermore, the highest carotenoid yields were achieved at high agitation rates (700 rpm), with carotenoid levels reaching nearly 120 µg/mL. Several carotenoids were identified, including β-carotene, γ-carotene, torularhodin, and torulene, with β-carotene being the major carotenoid, accounting for up to 70% of the total carotenoid content. The carotenoid-rich extract produced by R. toruloides under evaluated conditions was successfully incorporated into soap formulations, demonstrating the addition of antioxidant properties. This work provides a comprehensive understanding of xylose conversion into natural carotenoids by R. toruloides, presenting a promising avenue for their application in cosmetics. Furthermore, this study highlights the potential of a renewable and cost-effective approach for carotenoid production in the soap industry.
Solvent-Free Enzymatic Synthesis of Dietary Triacylglycerols from Cottonseed Oil in a Fluidized Bed Reactor Daniela Remonatto, Núbia Santaella, Lindomar Alberto Lerin, Juliana Cristina Bassan, Marcel Otávio Cerri, et al. Molecules, 2023 The synthesis of structured lipids with nutraceutical applications, such as medium-long-medium (MLM) triacylglycerols, via modification of oils and fats represents a challenge for the food industry. This study aimed to synthesize MLM-type dietary triacylglycerols by enzymatic acidolysis of cottonseed oil and capric acid (C10) catalyzed by Lipozyme RM IM (lipase from Rhizomucor miehei) in a fluidized bed reactor (FBR). After chemical characterization of the feedstock and hydrodynamic characterization of the reactor, a 22 central composite rotatable design was used to optimize capric acid incorporation. The independent variables were cycle number (20–70) and cottonseed oil/capric acid molar ratio (1:2–1:4). The temperature was set at 45 °C. The best conditions, namely a 1:4 oil/acid molar ratio and 80 cycles (17.34 h), provided a degree of incorporation of about 40 mol%, as shown by compositional analysis of the modified oil. Lipozyme RM IM showed good operational stability (kd = 2.72 × 10−4 h−1, t1/2 = 2545.78 h), confirming the good reuse capacity of the enzyme in the acidolysis of cottonseed oil with capric acid. It is concluded that an FBR configuration is a promising alternative for the enzymatic synthesis of MLM triacylglycerols.
A fuzzy logic algorithm and different mathematical models applied during the saccharomyces cerevisiae cultivations 21st International Congress of Chemical and Process Engineering Chisa 2014 and 17th Conference on Process Integration Modelling and Optimisation for Energy Saving and Pollution Reduction Pres 2014, 2014
Comparing the performance of conventional and airlift bioreactor during the Saccharomyces cerevisiae cultivations 21st International Congress of Chemical and Process Engineering Chisa 2014 and 17th Conference on Process Integration Modelling and Optimisation for Energy Saving and Pollution Reduction Pres 2014, 2014
Modeling of clavulanic acid production by streptomyces clavuligerus in fed-batch cultivation with glycerol and ornithine feeding Xxii Interamerican Congress of Chemical Engineering Ciiq 2006 and V Argentinian Congress of Chemical Engineering Caiq 2006 Innovation and Management for Sustainable Development, 2006
Measurement of mass transfer coefficient under growth condition using aeration-step method in an airlift reactor Xxii Interamerican Congress of Chemical Engineering Ciiq 2006 and V Argentinian Congress of Chemical Engineering Caiq 2006 Innovation and Management for Sustainable Development, 2006
RECENT SCHOLAR PUBLICATIONS
Heat balance soft sensor for ethanol and substrate monitoring in very high gravity fed-batch fermentations with CO2 stripping VT Mazziero, IIK Veloso, BG Campos, MV Santos, AJG Cruz, AC Badino, ... Chemical Engineering Research and Design , 2026 2026
Assessing the Role of Molecular Sieves in Continuous Enzymatic Synthesis of Geranyl Acetate DG de Oliveira, D Remonatto, IM de Oliveira, BW Picão, JV Oliveira, ... 2026
Model-based optimization of stripping onset in Saccharomyces cerevisiae very-high-gravity ethanol fermentations IIK Veloso, VT Mazziero, DA Lemos, AJG Cruz, MO Cerri, AC Badino Biochemical Engineering Journal, 110057 , 2025 2025 Citations: 1
A hybrid approach for determining the volumetric oxygen transfer coefficient (kLa) via the gassing-out method: from a phenomenological sigmoid model to a data-driven temporal … VT Mazziero, IIK Veloso, MO Cerri 2025
Effect of geometrical modifications on the mixing and enzymatic conversion of structured lipids in internal-loop airlift bioreactors DG de Oliveira, VT Mazziero, BW Picão, RMMG Pontes, MN Esperança, ... Biochemical engineering journal 207, 109316 , 2024 2024 Citations: 10
Effect of impeller type on cellular morphology and production of clavulanic acid by Streptomyces clavuligerus MCC Bustamante, CLL Costa, MN Esperança, VT Mazziero, MO Cerri, ... Brazilian Journal of Microbiology 55 (2), 1167-1177 , 2024 2024 Citations: 7
Hydrodynamic comparison of different geometries of square cross-section airlift bioreactor using computational fluid dynamics MN Esperança, MO Cerri, VT Mazziero, R Béttega, AC Badino International Journal of Chemical Reactor Engineering 21 (10), 1291-1303 , 2023 2023 Citations: 3
Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives RM Ribeiro, BW Picão, DO Gonçalves, M Scontri, VT Mazziero, ... Fermentation 9 (9), 828 , 2023 2023 Citations: 12
3D printing and enzyme immobilization: An overview of current trends D Remonatto, BF Izidoro, VT Mazziero, BP Catarino, JFC do Nascimento, ... Bioprinting 33, e00289 , 2023 2023 Citations: 31
Effects of Operational and Geometric Variables of a Bubble Column Bioreactor on Ethanol Stripping with CO 2 : Liquid and Gas-Phase Monitoring TB Martins, MV Santos, IIK Veloso, KCS Rodrigues, MN Esperanca, ... Industrial & Engineering Chemistry Research 62 (36), 14186-14198 , 2023 2023 Citations: 1
Solvent-free enzymatic synthesis of dietary triacylglycerols from cottonseed oil in a fluidized bed reactor D Remonatto, N Santaella, LA Lerin, JC Bassan, MO Cerri, AV De Paula Molecules 28 (14), 5384 , 2023 2023 Citations: 15
Oxygen transfer and gas holdup in airlift bioreactors assembled with helical flow promoters BW Picão, DO Gonçalves, RM Ribeiro, MN Esperança, G Peixoto, ... Bioprocess and Biosystems Engineering 46 (5), 681-692 , 2023 2023 Citations: 10
Characterization of packed-bed in the downcomer of a concentric internal-loop airlift bioreactor VT Mazziero, VG Batista, DG de Oliveira, M Scontri, AV de Paula, ... Biochemical Engineering Journal 181, 108407 , 2022 2022 Citations: 8
Relation of shear stress and K L a on bikaverin production by Fusarium oxysporum CCT7620 in a bioreactor MC Dos Santos, MO Cerri, JL Bicas Bioprocess and biosystems engineering 45 (4), 733-740 , 2022 2022 Citations: 5
Enzymatic synthesis of structured lipids from grape seed ( Vitis vinifera L.) oil in associated packed bed reactors ISC Cozentino, MF Rodrigues, VT Mazziero, MO Cerri, DCU Cavallini, ... Biotechnology and Applied Biochemistry 69 (1), 101-109 , 2022 2022 Citations: 25
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors MN Esperança, MM Buffo, CE Mendes, GY Rodriguez, R Bettega, ... Biochemical Engineering Journal 178, 108308 , 2022 2022 Citations: 12
Targeting riboswitches with synthetic small RNAs for metabolic engineering MRCR Lins, LA da Silva Amorim, GG Correa, BW Picão, M Mack, ... Metabolic engineering 68, 59-67 , 2021 2021 Citations: 18
Individual effect of shear rate and oxygen transfer on clavulanic acid production by Streptomyces clavuligerus RM Ribeiro, MN Esperanca, APA Sousa, AB Neto, MO Cerri Bioprocess and biosystems engineering 44 (8), 1721-1732 , 2021 2021 Citations: 11
Enzymatic synthesis of structured lipids from grape seed ( Vitis vinifera L.) oil in associated packed bed reactors. I de Souza Correia Cozentino, M de Freitas Rodrigues, VT Mazziero, ... 2020
Comparison of Two Methods for Counting Molds in Fermentations Using the Production of Bikaverin by Fusarium oxysporum CCT7620 as a Model MC Dos Santos, WS da Silva, BF da Silva, MO Cerri, MPA Ribeiro, ... Current microbiology 77 (11), 3671-3679 , 2020 2020 Citations: 9
MOST CITED SCHOLAR PUBLICATIONS
Engineering microbial living therapeutics: the synthetic biology toolbox DB Pedrolli, NV Ribeiro, PN Squizato, VN de Jesus, DA Cozetto, ... Trends in biotechnology 37 (1), 100-115 , 2019 2019 Citations: 211
Determination of the average shear rate in a stirred and aerated tank bioreactor A Campesi, MO Cerri, CO Hokka, AC Badino Bioprocess and biosystems engineering 32 (2), 241-248 , 2009 2009 Citations: 80
Average shear rate for non-Newtonian fluids in a concentric-tube airlift bioreactor MO Cerri, L Futiwaki, CDF Jesus, AJG Cruz, AC Badino Biochemical Engineering Journal 39 (1), 51-57 , 2008 2008 Citations: 73
Comparison between average shear rates in conventional bioreactor with Rushton and Elephant ear impellers MCC Bustamante, MO Cerri, AC Badino Chemical Engineering Science 90, 92-100 , 2013 2013 Citations: 68
Oxygen transfer in three scales of concentric tube airlift bioreactors MO Cerri, AC Badino Biochemical Engineering Journal 51 (1-2), 40-47 , 2010 2010 Citations: 40
Enhanced immunization techniques to obtain highly specific monoclonal antibodies R de Almeida, CN Nakamura, M de Lima Fontes, E Deffune, SL Felisbino, ... MAbs 10 (1), 46-54 , 2018 2018 Citations: 37
Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors MO Cerri, AC Badino Bioprocess and biosystems engineering 35 (6), 977-984 , 2012 2012 Citations: 36
Prediction of mean bubble size in pneumatic reactors MO Cerri, JC Baldacin, AJG Cruz, CO Hokka, AC Badino Biochemical Engineering Journal 53 (1), 12-17 , 2010 2010 Citations: 32
3D printing and enzyme immobilization: An overview of current trends D Remonatto, BF Izidoro, VT Mazziero, BP Catarino, JFC do Nascimento, ... Bioprinting 33, e00289 , 2023 2023 Citations: 31
A new approach for k L a determination by gassing‐out method in pneumatic bioreactors MO Cerri, M Nordi Esperança, A Colli Badino, ... Journal of Chemical Technology & Biotechnology 91 (12), 3061-3069 , 2016 2016 Citations: 31
Purification of clavulanic acid produced by Streptomyces clavuligerus via submerged fermentation using polyethylene glycol/cholinium chloride aqueous two-phase systems P Panas, C Lopes, MO Cerri, SPM Ventura, VC Santos-Ebinuma, ... Fluid Phase Equilibria 450, 42-50 , 2017 2017 Citations: 28
Enzymatic synthesis of structured lipids from grape seed ( Vitis vinifera L.) oil in associated packed bed reactors ISC Cozentino, MF Rodrigues, VT Mazziero, MO Cerri, DCU Cavallini, ... Biotechnology and Applied Biochemistry 69 (1), 101-109 , 2022 2022 Citations: 25
Average shear rate in three pneumatic bioreactors SS Thomasi, MO Cerri, AC Badino Bioprocess and biosystems engineering 33 (8), 979-988 , 2010 2010 Citations: 25
Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions WF da Silva Júnior, R Cano, AH Totola, LM de Carvalho, MO Cerri, ... Journal of Chromatography A 1395, 16-22 , 2015 2015 Citations: 20
Targeting riboswitches with synthetic small RNAs for metabolic engineering MRCR Lins, LA da Silva Amorim, GG Correa, BW Picão, M Mack, ... Metabolic engineering 68, 59-67 , 2021 2021 Citations: 18
Solvent-free enzymatic synthesis of dietary triacylglycerols from cottonseed oil in a fluidized bed reactor D Remonatto, N Santaella, LA Lerin, JC Bassan, MO Cerri, AV De Paula Molecules 28 (14), 5384 , 2023 2023 Citations: 15
Gas hold-up and oxygen mass transfer in three pneumatic bioreactors operating with sugarcane bagasse suspensions MN Esperança, FM Cunha, MO Cerri, TC Zangirolami, CS Farinas, ... Bioprocess and biosystems engineering 37 (5), 805-812 , 2014 2014 Citations: 15
Screening of medium constituents for clavulanic acid production by Streptomyces clavuligerus KCS Rodrigues, AT Souza, AC Badino, DB Pedrolli, MO Cerri brazilian journal of microbiology 49 (4), 832-839 , 2018 2018 Citations: 14
Synergistic Effects of Stirring and Aeration Rate on Carotenoid Production in Yeast Rhodotorula toruloides CCT 7815 Envisioning Their Application as Soap Additives RM Ribeiro, BW Picão, DO Gonçalves, M Scontri, VT Mazziero, ... Fermentation 9 (9), 828 , 2023 2023 Citations: 12
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors MN Esperança, MM Buffo, CE Mendes, GY Rodriguez, R Bettega, ... Biochemical Engineering Journal 178, 108308 , 2022 2022 Citations: 12
