SCUOLA DI MEDICINA UNIVERSIT' DEGLI STUDI DI BARI - DIPARTIMENTO DI MEDICINA DI PRECISIONE E RIGENERATIVA E AREA JONICA - DIMEPRE-J UNIVERSITA' DEGLI STUDI DI BARI
Impact of mechanical unloading on the irisin precursor, FNDC5, in skeletal muscle of sheep as a large animal experimental model Clelia Suriano, Onofrio Daniele Terrevoli, Francesco Staffieri, Luca Lacitignola, Antonio Crovace, Luisa Pellicani, Michela Taurino, Silvia Colucci, Maria Grano, Alberto Maria Crovace, Graziana Colaianni Jbmr Plus, 2026 Scientific evidence has demonstrated the involvement of irisin, a molecule produced by muscle contraction, in preventing the onset of musculoskeletal decline. We previously demonstrated that the expression of irisin and its muscle-derived precursor FNDC5 is affected by unloading in skeletal muscle of hindlimb-unloaded (HU) mice. Studies in the HU mouse model showed that treatment with irisin prevents muscle wasting and atrophy. In parallel, human studies documented a strong association between irisin/FNDC5 and muscle function. Therefore, the scientific community is committed to determining whether irisin may represent a promising clinical strategy for preventing and treating disorders caused by muscle inactivity, such as those affecting bedridden or elderly patients. In translational research from mice to humans, there are very few in vivo studies conducted on large animals. The sheep model is an excellent model for studying the musculoskeletal system due to its anatomical characteristics, which share similarities with humans, allowing for the reproduction of mechanical load distribution on the limbs. To provide translational knowledge on the progression of muscle atrophy, we evaluated the impact of unloading on the expression of FNDC5 and functional proteins of skeletal muscle in sheep. Our findings revealed a reduction of FNDC5 in the quadriceps muscle after 2 wk of immobilization. The FNDC5 decrease is accompanied by a reduction of myosin isoforms MyHC1 and MyHC2x, and an increase in muscle fibrosis. Importantly, FNDC5 expression positively correlated with expression of myosins, implying that the irisin/FNDC5 system is a driving force for muscle function in this large animal model.
Behavioral and Neurobiological Correlates of Resilience in the Hindlimb Unloading Mouse Model: A Longitudinal Ethogram with Neurotrophin Profile Arianna Racca, Patrizia Pignataro, Roberta Zerlotin, Graziana Esposito, Bijorn Omar Balzamino, Alessandra Micera, Maria Grano, Daniela Santucci Life, 2026 Among ground-based paradigms used to reproduce altered gravity exposure, the hindlimb unloading (HU) model is widely employed to simulate microgravity conditions by removing gravitational loading from the hindlimbs. Despite its extensive use, behavioral adjustments during suspension remain poorly characterized, although they may provide valuable indicators of animal welfare and individual susceptibility. Here, we comprehensively characterized the behavioral profile of mice during and after HU using a dedicated ethogram, with the aim of identifying behavioral markers associated with individual coping strategies. Several exploratory and postural behaviors showed marked time-dependent modulation, with baseline exploratory activity predicting a more adaptive behavioral trajectory during suspension, possibly indicative of greater resilience. In parallel, brain levels of the neurotrophins NGF and BDNF were measured to explore their relationship with behavioral outcomes. Although no significant group differences were detected, suspended mice displayed a progressive reduction in both neurotrophins over time, which paralleled behavioral adaptation. Together, these findings indicate that specific exploratory behaviors represent reliable predictors of resilience to HU, while NGF and BDNF may reflect ongoing neuroplastic processes associated with prolonged suspension.
Cerebrospinal fluid sclerostin levels in the early Alzheimer's disease stages Manuela Dicarlo, Patrizia Pignataro, Daniele Urso, Chiara Zecca, Maria Teresa Dell'Abate, Francesco Borlizzi, Valentina Gnoni, Alessia Giugno, Angela Oranger, Graziana Colaianni, Roberta Zerlotin, Clelia Suriano, Silvia Colucci, Giancarlo Logroscino, Maria Grano Alzheimer S and Dementia Diagnosis Assessment and Disease Monitoring, 2026 INTRODUCTION Sclerostin, a negative regulator of bone formation, has been involved in memory impairment in Alzheimer's disease (AD) mouse models and is increased in elderly people at risk of AD. Here, we investigated sclerostin's role across the clinical stages of AD. METHODS We evaluated cerebrospinal fluid (CSF) sclerostin levels in patients with dementia due to AD, mild cognitive impairment, and subjective memory complaints, biologically characterized via the amyloid/tau/neurodegeneration classification. Results were correlated with AD biomarkers, amyloid beta (Aβ) 42, phosphorylated tau (p‐tau), and total tau (t‐tau), and clinical parameters of dementia severity. RESULTS CSF sclerostin increased in patients with dementia due to AD and correlated negatively with Aβ42 and positively with p‐tau, t‐tau, and dementia severity. DISCUSSION The association of CSF sclerostin with Aβ42, tau pathology, and dementia severity in the early disease stages is of great clinical relevance for the identification of sclerostin as a promising biomarker in early AD stages.
Comparative psychological well-being evaluation among CKD patients on conservative therapy, dialysis, or kidney transplantation: a cross-sectional study Patrizia Pignataro, Simona Simone, Manuela Dicarlo, Federica Cassone, Gianvito Caggiano, Raffaella Guido, Fausta Piancone, Carmen Sivo, Anna Maria Dipalma, Roberto Russo, Marco Spilotros, Pasquale Di Tonno, Silvia Colucci, Graziana Colaianni, Paola Pontrelli, Maria Grano, Loreto Gesualdo Frontiers in Psychology, 2026 Background Chronic kidney disease (CKD) has a significant impact on psychological well-being. Here, the psychological evaluation of patients with CKD undergoing replacement treatment (dialysis or kidney transplantation) and conservative therapy (preemptive patients, who are waiting list for kidney transplantation) was analyzed. In addition, serum irisin levels, a protein displaying anxiolytic and antidepressant effects in mice, were measured in dialysis patients. Methods Dialysis ( N = 57), non-dialysis (preemptive, N = 31) and kidney transplant patients ( N = 33) were enrolled. All participants underwent psychometric tests including State–Trait Anxiety Inventory (STAI-Y 1 and 2 form), Psychological General Well-Being Index (PGWBI), Symptom Checklist-90-R (SCL-90-R), etc. Serum irisin levels in dialyzed patients were measured by ELISA assay. Results Dialysis patient group scored worse on all tests performed than both preemptive and kidney transplant patients. Indeed, dialysis patients displayed the lowest PGWBI score, and higher scores of BDI, and STAY-1 and STAY-2, compared with preemptive and kidney transplant patients. We also found that about 40% of dialysis patients showed significant psychological distress with higher clinical attention values in the somatization, obsessive-compulsive, depression, and anxiety domains assessed by SCL-90. Furthermore, the stratification of all patients into groups younger and older than 50 years showed that the older group of transplanted patients displayed better outcomes than the younger ones. Finally, stratification of dialysis patients according to irisin levels revealed that only those with higher serum irisin levels had better psychological conditions in tests. Conclusions Kidney transplantation as well as conservative therapy were related to a lower prevalence of depressive symptoms and other psychological disorders than dialysis. Furthermore, all transplanted patients over 50 years of age showed better outcomes than the younger ones. However, dialyzed patients with high levels of circulating irisin displayed better psychological conditions. Overall, our findings supported the importance to provide timely access to transplantation and to improve psychological support for dialysis patients.
Cortical Bone Loss and Fragility in a 2-Month Triple Transgenic Mouse Model of Alzheimer’s Disease Giuseppina Storlino, Francesca Posa, Teresa Stefania Dell'Endice, Federica Piccolo, Graziana Colaianni, Tommaso Cassano, Maria Grano, Giorgio Mori Cells, 2025 Alzheimer’s disease (AD) and osteoporosis frequently co-occur in the elderly; however, the pathophysiological link between these two diseases remains unclear. This study investigates skeletal alterations in a triple transgenic 3xTg-AD mouse model of AD (3xTg-AD), which harbors mutations in β-amyloid precursor protein (βAPPSwe), presenilin-1 (PS1M146V), and tauP301L, and recapitulates key aspects of AD pathology, including age-dependent β-amyloid plaque accumulation and cognitive decline. To assess early skeletal changes, we analyzed femurs and tibiae of 2-month-old male non-Tg and 3xTg-AD mice (n = 9/group) using micro-CT. Despite the absence of β-amyloid plaques at this stage, 3xTg-AD mice showed significant cortical bone loss, with reduced bone surface, periosteal and endosteal perimeters, total and cortical cross-sectional area, and polar moment of inertia. The 3-point-bending test confirmed compromised mechanical properties, including reduced maximum load-to-fracture and stiffness. Histological analyses highlighted an increased number of Empty Osteocyte Lacunae, reduced TRAP+ osteocytes, and an elevated number of osteoclasts; such evidence indicates impaired osteocyte function and increased bone resorption. These findings indicate that cortical bone loss and compromised mechanical properties occur before detectable neuropathological hallmarks in this AD model.
Correlation Between Irisin and Cognitive Functions in Alzheimer Dementia Patrizia Pignataro, Manuela Dicarlo, Chiara Zecca, Daniele Urso, Maria Teresa Dell'Abate, Davide Vilella, Francesco Borlizzi, Roberta Zerlotin, Angela Oranger, Graziana Colaianni, Silvia Colucci, Maria Grano, Giancarlo Logroscino Annals of Clinical and Translational Neurology, 2025 ObjectiveThe myokine irisin, a recent positive mediator of exercise in the brain, shows neuroprotective functions against Alzheimer's disease (AD). The association between irisin and cognition has never been explored in a biologically defined cohort of patients. Thus, in the present study, we investigated the association of irisin with multidomain cognition in patients showing dementia‐related symptomatology.MethodsCerebrospinal fluid (CSF) and serum irisin levels were evaluated using enzyme‐linked immunoassays in a cohort of subjects with a confirmed biomarker evidence of AD, including AD (n = 82), mild cognitive impairment (MCI, n = 44), and subjective memory complaint (SMC, n = 20) patients. The results of this analysis were correlated with global cognitive efficiency assessed by Mini‐Mental State Examination, and multidomain cognition evaluated by a battery of psychometric tests.ResultsDecreased CSF and serum irisin levels were observed in AD and MCI patients compared to SMC. A significant correlation has been found between irisin in the CSF and global cognitive efficiency, as well as with specific cognitive domains such as memory, executive functions, attention, visuospatial abilities, and language. For serum irisin, the correlation analysis evidenced similar results to those observed for the CSF.InterpretationOur results highlight the key involvement of irisin in multidomain cognition, indicating its potential role as a cognitive biomarker of AD progression.
Irisin Prevents the Effects of Simulated Microgravity on Bone and Muscle Differentiation Markers Lorenzo Sanesi, Roberta Zerlotin, Alessio Campiolo, Angela Oranger, Manuela Dicarlo, et al. FASEB Bioadvances, 2025 Microgravity exposure affects both tissues and cells, and, in this regard, one of the most affected targets is the skeletal muscle system due to the significant loss of bone and muscle mass leading to osteoporosis and sarcopenia, respectively. Several efforts are underway to counteract the effects of microgravity, and recent studies on irisin, a myokine with anabolic effects on the musculoskeletal system, have shown promising results. Due to the practical challenges of conducting experiments in actual microgravity, different devices generating a simulated microgravity condition on Earth have been developed. Here, we exposed myoblasts, osteoblasts, osteocytes to a random position machine (RPM) for five days to assess microgravity effect on the expression of key differentiation factors in cells untreated or treated with irisin. In myoblasts (C2C12), exposure to RPM led to increased expression of early myogenesis maker genes Pax7 ( p = 0.0016), Myf5 ( p = 0.0005) and MyoD ( p = 0.0009). Irisin treatment in the last 8 h of RPM cultures prevented these increases by returning Pax7 ( p = 0.0008) and MyoD ( p = 0.01) to control values, and only partially Myf5 . In bone cells, exposure to RPM for 5 days showed no effect in osteoblasts (MC3T3) but decreased the expression of Pdpn ( p = 0.0285) and Dmp‐1 ( p = 0.0423) genes in osteocytes (MLO‐Y4). Irisin treatment completely prevented the decline in Pdpn ( p = 0.293) and Dmp‐1 ( p = 0.0339) levels. Overall, our data showed that the impact of RPM exposure keeps myoblasts and osteocytes in a proliferative state, and irisin treatment restores them to their baseline biological condition, suggesting that irisin can counteract the changes induced by simulated microgravity.
Influence of Non-Cross-Linking AGEs on Mechanical Properties and Morphological Features of Tropocollagen Peptides: A Molecular Dynamics Study Yu-Bai Xiao, Linda Ravazzano, Maria Grano, Graziana Colaianni, Clair Baldock, Flavia Libonati, Anna Tarakanova ACS Biomaterials Science and Engineering, 2025 Collagen, a protein known for its long lifespan, is susceptible to accumulation of advanced glycation end products (AGEs) with age. These AGEs are considered markers that indicate the aging severity and influence the mechanics of tissues, leading to fragile bones and hardened skin. While many cross-linking AGEs have been widely studied for their ability to reduce the elasticity of biological tissues, contributing to skin hardening and fragile bones, through strong covalent bonds, non-cross-linking AGEs, or AGE adducts, are typically investigated as indicators of aging or as signaling factors in pathological conditions. However, recent experimental findings have revealed that the number of AGE adducts in aged bone is comparable to enzymatic cross-links, which are significantly more abundant than cross-linking AGEs. Based on these observations, we consider one of the most abundant AGE adducts - carboxymethyllysine (CML) - and employ molecular dynamics simulations to explore its direct impact on the mechanical and conformational properties of single tropocollagen molecules. Our models demonstrate that tropocollagen peptides, constructed based on sequences experimentally identified with sites of CML modifications in type I collagen derived from human cortical bone, exhibit heterogeneous behaviors under tensile testing. Still, most of these modified peptides display compromised structural stability, reduction in structural strength, and diminished energy dissipation ability when tension is applied. This study highlights the potential impact of non-cross-linking AGEs on collagen behavior at molecular scale and provides insights into the mechanisms underlying these modifications. Gaining a deeper understanding of the role of AGE adducts and their contribution to the aging process may pave the way for future solutions in antiaging research.
Reference Intervals (RIs) of the Bone Turnover Markers (BTMs) in Children and Adolescents: A Proposal for Effective Use Vincenzo Brescia, Roberto Lovero, Antonietta Fontana, Roberta Zerlotin, Silvia Concetta Colucci, Maria Grano, Angela Pia Cazzolla, Francesca Di Serio, Vito Crincoli, Maria Felicia Faienza Biomedicines, 2025 Background/Objectives: Bone turnover markers (BTMs) can provide information on the bone growth of apparently healthy children and adolescents or useful results in the diagnosis and monitoring of the disease condition, comparing them with appropriate reference intervals (RIs). The aim of this study was to establish the RI for the BTM [specific bone alkaline phosphatase (BALP), carboxy-terminal cross-linked collagen type I telopeptide (CTX), N-terminal propeptide pro-collagen type I (PINP), osteocalcin (OC), resistant to acid tartrate phosphatase isoform 5b (TRAcP-5b)] on serum samples from children and adolescents. Method: 202 samples from children and adolescents (ages 1–18 years) (51.48% male), considered apparently healthy. The biomarker was analyzed on automatic immunometric equipment (TGSTA Technogenetics) and the IDS-iSYS automated system kits The RI of the studied parameters was calculated according to CLSI Guideline C28-A3 with stratification by age and sex. Evaluation of the distribution of values and the meaning of the biomarker concentrations were used to calculate general and specific RI for an age group. Results: BTM concentrations vary with pubertal growth. The pattern of change differs for each bone marker. General and age-specific RI were calculated: 1–14 years, 15–18 years for BALP and CTX; 1–13 years, 14–18 years for Oc and PINP and 1–12 years, 13–18 years for TRAcP. Discussion and Conclusions: Concentrations for biomarker studied vary with age and gender. The proof of concentrations with insignificant changes until puberty led to identification of two groups of RI relating to the covariables (age and sex) for each biomarker.
Alteration of vasopressin-aquaporin system in hindlimb unloading mice Marianna Ranieri, Maria Venneri, Giuseppina Storlino, Angela Ferrulli, Mariagrazia D’Agostino, Mariangela Centrone, Annarita Di Mise, Roberta Zerlotin, Grazia Tamma, Maria Grano, Giovanna Valenti Frontiers in Physiology, 2025 Murine hindlimb unloading (HU) is considered a model of choice for simulating the physiological effects of microgravity on several functions, including fluid and electrolyte homeostasis. Microgravity causes changes in blood redistribution, modulating vasopressin secretion, a major hormone controlling water reabsorption through the vasopressin-sensitive water channel AQP2. In this study, mice were hindlimb suspended over 4 weeks or rested in the ground as controls, and vasopressin levels, along with renal aquaporins expression were investigated. Copeptin, a stable precursor of the hormone vasopressin, significantly increased as early as 1 week of unloading which correlated with a significant increase in AQP2 total protein expression and decrease in serum osmolality, suggesting early activation of the vasopressin/AQP2 axis in this model. Conversely, in 4 weeks HU suspended mice, copeptin decreased significantly and both AQP2 mRNA and AQP2 total protein expression were significantly reduced. Consistent with a downregulation of the vasopressin/AQP2 axis an increase in serum osmolality was observed at 4 weeks HU. The basolateral water channels AQP3 and AQP4 were, on the other hand, unaffected. Immunolocalization studies confirmed reduced expression of AQP2 in renal collecting ducts of HU mice at 4 weeks. A significantly increased amount of the expressed AQP2 was found phosphorylated at Ser261, a site regulating AQP2 protein stability and degradation. In line, p38-MAPK, committed to phosphorylate Ser261 and to increase miR137 expression, an AQP2 mRNA-targeted microRNA, was significantly increased in HU, suggesting that reduced AQP2 expression was mainly due to increased protein degradation and downregulation of AQP2-mRNA translation. Our results suggest that vasopressin/AQP2 axis is upregulated as early as 1 week and may be involved in the antidiuretic response also observed in early spaceflight period in astronauts. Contrariwise, the vasopressin-AQP2 system is downregulated after 4 weeks HU, likely to counteract the persistent central venous pressure due to cephalic shift of fluids.
Irisin and Metastatic Melanoma: Selective Anti-Invasiveness Activity in BRAF Wild-Type Cells Simona Serratì, Roberta Zerlotin, Michele Manganelli, Roberta Di Fonte, Manuela Dicarlo, Angela Oranger, Graziana Colaianni, Letizia Porcelli, Amalia Azzariti, Stefania Guida, Maria Grano, Silvia Concetta Colucci, Gabriella Guida International Journal of Molecular Sciences, 2025
How to obtain an integrated picture of the molecular networks involved in adaptation to microgravity in different biological systems? Craig R. G. Willis, Marco Calvaruso, Debora Angeloni, Sarah Baatout, Alexandra Benchoua, Juergen Bereiter-Hahn, Daniele Bottai, Judith-Irina Buchheim, Eugénie Carnero-Diaz, Sara Castiglioni, Duccio Cavalieri, Gabriele Ceccarelli, Alexander Chouker, Francesca Cialdai, Gianni Ciofani, Giuseppe Coppola, Gabriella Cusella, Andrea Degl’Innocenti, Jean-Francois Desaphy, Jean-Pol Frippiat, Michael Gelinsky, Giada Genchi, Maria Grano, Daniela Grimm, Alain Guignandon, Raúl Herranz, Christine Hellweg, Carlo Saverio Iorio, Thodoris Karapantsios, Jack van Loon, Matteo Lulli, Jeanette Maier, Jos Malda, Emina Mamaca, Lucia Morbidelli, Andreas Osterman, Aleksandr Ovsianikov, Francesco Pampaloni, Elizabeth Pavezlorie, Veronica Pereda-Campos, Cyrille Przybyla, Petra Rettberg, Angela Maria Rizzo, Kate Robson-Brown, Leonardo Rossi, Giorgio Russo, Alessandra Salvetti, Chiara Risaliti, Daniela Santucci, Matthias Sperl, Kevin Tabury, Sara Tavella, Christiane Thielemann, Ronnie Willaert, Monica Monici, Nathaniel J. Szewczyk Npj Microgravity, 2024
How are cell and tissue structure and function influenced by gravity and what are the gravity perception mechanisms? Trent Davis, Kevin Tabury, Shouan Zhu, Debora Angeloni, Sarah Baatout, Alexandra Benchoua, Juergen Bereiter-Hahn, Daniele Bottai, Judith-Irina Buchheim, Marco Calvaruso, Eugénie Carnero-Diaz, Sara Castiglioni, Duccio Cavalieri, Gabriele Ceccarelli, Alexander Choukér, Francesca Cialdai, Gianni Ciofani, Giuseppe Coppola, Gabriella Cusella, Andrea Degl’Innocenti, Jean-Francois Desaphy, Jean-Pol Frippiat, Michael Gelinsky, Giada Genchi, Maria Grano, Daniela Grimm, Alain Guignandon, Christiane Hahn, Jason Hatton, Raúl Herranz, Christine E. Hellweg, Carlo Saverio Iorio, Thodoris Karapantsios, Jack J.W.A. van Loon, Matteo Lulli, Jeanette Maier, Jos Malda, Emina Mamaca, Lucia Morbidelli, Angelique van Ombergen, Andreas Osterman, Aleksandr Ovsianikov, Francesco Pampaloni, Elizabeth Pavezlorie, Veronica Pereda-Campos, Cyrille Przybyla, Christopher Puhl, Petra Rettberg, Angela Maria Rizzo, Kate Robson-Brown, Leonardo Rossi, Giorgio Russo, Alessandra Salvetti, Daniela Santucci, Matthias Sperl, Sara Tavella, Christiane Thielemann, Ronnie Willaert, Nathaniel Szewczyk, Monica Monici Npj Microgravity, 2024
LIGHT/TNFSF14 Affects Adipose Tissue Phenotype Angela Oranger, Graziana Colaianni, Giuseppe Ingravallo, Vincenza Sara Scarcella, Maria Felicia Faienza, Maria Grano, Silvia Colucci, Giacomina Brunetti International Journal of Molecular Sciences, 2024
How do gravity alterations affect animal and human systems at a cellular/tissue level? Francesca Cialdai, Austin M. Brown, Cory W. Baumann, Debora Angeloni, Sarah Baatout, Alexandra Benchoua, Juergen Bereiter-Hahn, Daniele Bottai, Judith-Irina Buchheim, Marco Calvaruso, Eugénie Carnero-Diaz, Sara Castiglioni, Duccio Cavalieri, Gabriele Ceccarelli, Alexander Choukér, Gianni Ciofani, Giuseppe Coppola, Gabriella Cusella, Andrea Degl’Innocenti, Jean-Francois Desaphy, Jean-Pol Frippiat, Michael Gelinsky, Giada Genchi, Maria Grano, Daniela Grimm, Alain Guignandon, Christiane Hahn, Jason Hatton, Raúl Herranz, Christine E. Hellweg, Carlo Saverio Iorio, Thodoris Karapantsios, Jack van Loon, Matteo Lulli, Jeanette Maier, Jos Malda, Emina Mamaca, Lucia Morbidelli, Angelique van Ombergen, Andreas Osterman, Aleksandr Ovsianikov, Francesco Pampaloni, Elizabeth Pavezlorie, Veronica Pereda-Campos, Cyrille Przybyla, Christopher Puhl, Petra Rettberg, Chiara Risaliti, Angela Maria Rizzo, Kate Robson-Brown, Leonardo Rossi, Giorgio Russo, Alessandra Salvetti, Daniela Santucci, Matthias Sperl, Felice Strollo, Kevin Tabury, Sara Tavella, Christiane Thielemann, Ronnie Willaert, Nathaniel J. Szewczyk, Monica Monici Npj Microgravity, 2023
Irisin and Secondary Osteoporosis in Humans Roberta Zerlotin, Angela Oranger, Patrizia Pignataro, Manuela Dicarlo, Filippo Maselli, Giorgio Mori, Silvia Concetta Colucci, Maria Grano, Graziana Colaianni International Journal of Molecular Sciences, 2022
Systemic administration of recombinant irisin accelerates fracture healing in mice Silvia Concetta Colucci, Cinzia Buccoliero, Lorenzo Sanesi, Mariella Errede, Graziana Colaianni, Tiziana Annese, Mohd Parvez Khan, Roberta Zerlotin, Manuela Dicarlo, Ernestina Schipani, Kenneth M. Kozloff, Maria Grano International Journal of Molecular Sciences, 2021
The effect of Irisin on bone cells in vivo and in vitro Cinzia Buccoliero, Angela Oranger, Graziana Colaianni, Patrizia Pignataro, Roberta Zerlotin, Roberto Lovero, Mariella Errede, Maria Grano Biochemical Society Transactions, 2021
The novel role of PGC1α in bone metabolism Cinzia Buccoliero, Manuela Dicarlo, Patrizia Pignataro, Francesco Gaccione, Silvia Colucci, Graziana Colaianni, Maria Grano International Journal of Molecular Sciences, 2021
Novel insights in health-promoting properties of sweet cherries Maria Felicia Faienza, Filomena Corbo, Alessia Carocci, Alessia Catalano, Maria Lisa Clodoveo, Maria Grano, David Q.-H. Wang, Gabriele D'Amato, Marilena Muraglia, Carlo Franchini, Giacomina Brunetti, Piero Portincasa Journal of Functional Foods, 2020
Irisin Prevents Disuse-Induced Osteocyte Apoptosis Giuseppina Storlino, Graziana Colaianni, Lorenzo Sanesi, Luciana Lippo, Giacomina Brunetti, Mariella Errede, Silvia Colucci, Giovanni Passeri, Maria Grano Journal of Bone and Mineral Research, 2020
LIGHT/TNFSF14 regulates estrogen deficiency-induced bone loss Giacomina Brunetti, Giuseppina Storlino, Angela Oranger, Graziana Colaianni, Maria F Faienza, Giuseppe Ingravallo, Mariasevera Di Comite, Janne E Reseland, Monica Celi, Umberto Tarantino, Giovanni Passeri, Carl F Ware, Maria Grano, Silvia Colucci Journal of Pathology, 2020
LIGHT/TNFSF14 Promotes Osteolytic Bone Metastases in Non-small Cell Lung Cancer Patients Giacomina Brunetti, Dimas C Belisario, Sara Bortolotti, Giuseppina Storlino, Graziana Colaianni, Maria F Faienza, Lorenzo Sanesi, Valentina Alliod, Lucio Buffoni, Elisa Centini, Claudia Voena, Roberta Pulito, Silvia Novello, Giuseppe Ingravallo, Rita Rizzi, Giorgio Mori, Janne E Reseland, Carl F Ware, Silvia Colucci, Riccardo Ferracini, Maria Grano, Ilaria Roato Journal of Bone and Mineral Research, 2020
Effects of sweet cherry polyphenols on enhanced osteoclastogenesis associated with childhood obesity Filomena Corbo, Giacomina Brunetti, Pasquale Crupi, Sara Bortolotti, Giuseppina Storlino, Laura Piacente, Alessia Carocci, Alessia Catalano, Gualtiero Milani, Graziana Colaianni, Silvia Colucci, Maria Grano, Carlo Franchini, Maria Lisa Clodoveo, Gabriele D'Amato, Maria Felicia Faienza Frontiers in Immunology, 2019
Deletion of the Transcription Factor PGC-1α in Mice Negatively Regulates Bone Mass Graziana Colaianni, Luciana Lippo, Lorenzo Sanesi, Giacomina Brunetti, Monica Celi, Nunzio Cirulli, Giovanni Passeri, Janne Reseland, Ernestina Schipani, Maria Felicia Faienza, Umberto Tarantino, Silvia Colucci, Maria Grano Calcified Tissue International, 2018
Mechanisms of Enhanced Osteoclastogenesis in Alkaptonuria Giacomina Brunetti, Albina Tummolo, Gabriele D'Amato, Alberto Gaeta, Federica Ortolani, Laura Piacente, Paola Giordano, Silvia Colucci, Maria Grano, Francesco Papadia, Maria F. Faienza American Journal of Pathology, 2018
Impairment of Bone Remodeling in LIGHT/TNFSF14-Deficient Mice Giacomina Brunetti, Maria Felicia Faienza, Graziana Colaianni, Isabella Gigante, Angela Oranger, Paolo Pignataro, Giuseppe Ingravallo, Adriana Di Benedetto, Sara Bortolotti, Mariasevera Di Comite, Giuseppina Storlino, Luciana Lippo, Lindsay Ward-Kavanagh, Giorgio Mori, Janne E Reseland, Giovanni Passeri, Ernestina Schipani, Koji Tamada, Carl F Ware, Silvia Colucci, Maria Grano Journal of Bone and Mineral Research, 2018
Irisin prevents and restores bone loss and muscle atrophy in hind-limb suspended mice Graziana Colaianni, Teresa Mongelli, Concetta Cuscito, Paolo Pignataro, Luciana Lippo, Giovanna Spiro, Angela Notarnicola, Ilenia Severi, Giovanni Passeri, Giorgio Mori, Giacomina Brunetti, Biagio Moretti, Umberto Tarantino, Silvia C. Colucci, Janne E. Reseland, Roberto Vettor, Saverio Cinti, Maria Grano Scientific Reports, 2017
Irisin levels correlate with bone mineral density in soccer players Journal of Biological Regulators and Homeostatic Agents, 2017
Sclerostin stimulates angiogenesis in human endothelial cells Angela Oranger, Giacomina Brunetti, Graziana Colaianni, Roberto Tamma, Claudia Carbone, Luciana Lippo, Giorgio Mori, Paolo Pignataro, Nunzio Cirulli, Roberta Zerlotin, Biagio Moretti, Angela Notarnicola, Domenico Ribatti, Maria Grano, Silvia Colucci Bone, 2017
Irisin and musculoskeletal health Graziana Colaianni, Saverio Cinti, Silvia Colucci, Maria Grano Annals of the New York Academy of Sciences, 2017
NURR1 downregulation favors osteoblastic differentiation of MSCs Adriana Di Benedetto, Francesca Posa, Claudia Carbone, Stefania Cantore, Giacomina Brunetti, Matteo Centonze, Maria Grano, Lorenzo Lo Muzio, Elisabetta A. Cavalcanti-Adam, Giorgio Mori Stem Cells International, 2017
The effects of bone pâté on human osteoblasts cell cultures Nicola Quaranta, Cinzia Buccoliero, Concetta De Luca, Giorgio Mori, Giacomina Brunetti, Silvia Colucci, Graziana Colaianni, Maria Grano European Archives of Oto Rhino Laryngology, 2016
Functions of vasopressin and oxytocin in bone mass regulation Li Sun, Roberto Tamma, Tony Yuen, Graziana Colaianni, Yaoting Ji, Concetta Cuscito, Jack Bailey, Samarth Dhawan, Ping Lu, Cosima D. Calvano, Ling-Ling Zhu, Carlo G. Zambonin, Adriana Di Benedetto, Agnes Stachnik, Peng Liu, Maria Grano, Silvia Colucci, Terry F. Davies, Maria I. New, Alberta Zallone, Mone Zaidi Proceedings of the National Academy of Sciences of the United States of America, 2016
The myokine Irisin recapitulates the effect of physical activity on bone and muscle tissues Journal of Gerontology and Geriatrics, 2016
High serum sclerostin levels in children with haemophilia A Paola Giordano, Giacomina Brunetti, Giuseppe Lassandro, Lucia D. Notarangelo, Matteo Luciani, Rosa M. Mura, Ilaria Lazzareschi, Elena Santagostino, Laura Piacente, Annamaria Ventura, Luciano Cavallo, Maria Grano, Maria F. Faienza British Journal of Haematology, 2016
Mechanisms of enhanced osteoclastogenesis in girls and young women with Turner's Syndrome Maria Felicia Faienza, Giacomina Brunetti, Annamaria Ventura, Laura Piacente, Maria Francesca Messina, Filippo De Luca, Maria Ciccarelli, Angela Oranger, Giorgio Mori, Maria Pia Natale, Margherita Gigante, Elena Ranieri, Loreto Gesualdo, Silvia Colucci, Luciano Cavallo, Maria Grano Bone, 2015
The myokine irisin increases cortical bone mass Graziana Colaianni, Concetta Cuscito, Teresa Mongelli, Paolo Pignataro, Cinzia Buccoliero, Peng Liu, Ping Lu, Loris Sartini, Mariasevera Di Comite, Giorgio Mori, Adriana Di Benedetto, Giacomina Brunetti, Tony Yuen, Li Sun, Janne E. Reseland, Silvia Colucci, Maria I. New, Mone Zaidi, Saverio Cinti, Maria Grano Proceedings of the National Academy of Sciences of the United States of America, 2015
Cellular mechanisms of bone regeneration: Role of WNT-1 in bone-muscle interaction during physical activity Journal of Biological Regulators and Homeostatic Agents, 2015
Skeleton and glucose metabolism: A bone-pancreas loop Maria Felicia Faienza, Vincenza Luce, Annamaria Ventura, Graziana Colaianni, Silvia Colucci, Luciano Cavallo, Maria Grano, Giacomina Brunetti International Journal of Endocrinology, 2015
Osteoblast regulation via ligand-activated nuclear trafficking of the oxytocin receptor Adriana Di Benedetto, Li Sun, Carlo G. Zambonin, Roberto Tamma, Beatrice Nico, Cosima D. Calvano, Graziana Colaianni, Yaoting Ji, Giorgio Mori, Maria Grano, Ping Lu, Silvia Colucci, Tony Yuen, Maria I. New, Alberta Zallone, Mone Zaidi Proceedings of the National Academy of Sciences of the United States of America, 2014
Introduction Maria Grano, Graziana Colaianni Clinical Reviews in Bone and Mineral Metabolism, 2013
Osteotropic cancers: From primary tumor to bone Giacomina Brunetti, Graziana Colaianni, Maria Felicia Faienza, Silvia Colucci, Maria Grano Clinical Reviews in Bone and Mineral Metabolism, 2013
Regulation of bone remodeling by vasopressin explains the bone loss in hyponatremia Roberto Tamma, Li Sun, Concetta Cuscito, Ping Lu, Michelangelo Corcelli, Jianhua Li, Graziana Colaianni, Surinder S. Moonga, Adriana Di Benedetto, Maria Grano, Silvia Colucci, Tony Yuen, Maria I. New, Alberta Zallone, Mone Zaidi Proceedings of the National Academy of Sciences of the United States of America, 2013
Osteogenic differentiation of dental follicle stem cells Giorgio Mori, Andrea Ballini, Claudia Carbone, Angela Oranger, Giacomina Brunetti, Adriana Di Benedetto, Biagio Rapone, Stefania Cantore, Mariasevera Di Comite, Silvia Colucci, Maria Grano, Felice R. Grassi International Journal of Medical Sciences, 2012
Bone marrow oxytocin mediates the anabolic action of estrogen on the skeleton Graziana Colaianni, Li Sun, Adriana Di Benedetto, Roberto Tamma, Ling-Ling Zhu, Jay Cao, Maria Grano, Tony Yuen, Sylvia Colucci, Concetta Cuscito, Lucia Mancini, Jianhua Li, Katsuhiko Nishimori, Itai Bab, Heon-Jin Lee, Jameel Iqbal, W.Scott Young, Clifford Rosen, Alberta Zallone, Mone Zaidi Journal of Biological Chemistry, 2012
Osteoclastogenesis and arthritis Nicola Maruotti, Maria Grano, Silvia Colucci, Francesca d’Onofrio, Francesco Paolo Cantatore Clinical and Experimental Medicine, 2011
Regulated production of the pituitary hormone oxytocin from murine and human osteoblasts Graziana Colaianni, Adriana Di Benedetto, Ling-Ling Zhu, Roberto Tamma, Jianhua Li, Giovanni Greco, Yuanzhen Peng, Stefania Dell’Endice, Guangyu Zhu, Concetta Cuscito, Maria Grano, Silvia Colucci, Jameel Iqbal, Tony Yuen, Li Sun, Mone Zaidi, Alberta Zallone Biochemical and Biophysical Research Communications, 2011
TRAIL effect on osteoclast formation in physiological and pathological conditions Frontiers in Bioscience Elite, 2011
Dental pulp stem cells: Osteogenic differentiation and gene expression Giorgio Mori, Giacomina Brunetti, Angela Oranger, Claudia Carbone, Andrea Ballini, Lorenzo Lo Muzio, Silvia Colucci, Claudio Mori, Felice Roberto Grassi, Maria Grano Annals of the New York Academy of Sciences, 2011
Oxytocin is an anabolic bone hormone Roberto Tamma, Graziana Colaianni, Ling-ling Zhu, Adriana DiBenedetto, Giovanni Greco, Gabriella Montemurro, Nicola Patano, Maurizio Strippoli, Rosaria Vergari, Lucia Mancini, Silvia Colucci, Maria Grano, Roberta Faccio, Xuan Liu, Jianhua Li, Sabah Usmani, Marilyn Bachar, Itai Bab, Katsuhiko Nishimori, Larry J. Young, Christoph Buettner, Jameel Iqbal, Li Sun, Mone Zaidi, Alberta Zallone Proceedings of the National Academy of Sciences of the United States of America, 2009
Chlamydia pneumoniae and atherosclerosis: The role of mast cells Journal of Biological Regulators and Homeostatic Agents, 2009
Alteration of activity and survival of osteoblasts obtained from human periodontitis patients: Role of TRAIL Journal of Biological Regulators and Homeostatic Agents, 2007
TRAIL is involved in human osteoclast apoptosis GIACOMINA BRUNETTI, ANGELA ORANGER, GIORGIO MORI, ROBERTO TAMMA, ADRIANA DI BENEDETTO, PAOLO PIGNATARO, FELICE R. GRASSI, ALBERTA ZALLONE, MARIA GRANO, SILVIA COLUCCI Annals of the New York Academy of Sciences, 2007
Gorham-stout syndrome: A monocyte-mediated cytokine propelled disease Silvia Colucci, Giulia Taraboletti, Luca Primo, Andrea Viale, Cristina Roca, Donatella Valdembri, Massimo Geuna, Marco Pagano, Maria Grano, Anthony M Pogrel, Adrian L Harris, Nicholas N Athanasou, Alberto Mantovani, Alberta Zallone, Federico Bussolino Journal of Bone and Mineral Research, 2006
The role of OPG/TRAIL complex in multiple myeloma GIACOMINA BRUNETTI, SILVIA COLUCCI, RITA RIZZI, GIORGIO MORI, GRAZIANA COLAIANNI, ANGELA ORANGER, ALBERTA ZALLONE, VINCENZO LISO, MARIA GRANO Annals of the New York Academy of Sciences, 2006
Interleukin-7 production by B lymphocytes affects the T cell-dependent osteoclast formation in an in vitro model derived from human periodontitis patients. International Journal of Immunopathology and Pharmacology, 2005
Human osteoclasts express oxytocin receptor Silvia Colucci, Graziana Colaianni, Giorgio Mori, Maria Grano, Alberta Zallone Biochemical and Biophysical Research Communications, 2002
HGF and M-CSF modulate adhesion of MDA-231 breast cancer cell by increasing osteopontin secretion Journal of Biological Regulators and Homeostatic Agents, 2002
Human osteoclast-like cells selectively recognize laminin isoforms, an event that induces migration and activates Ca2+ mediated signals Journal of Cell Science, 1996
Etidronate inhibits osteoclast adhesion to bone surfaces but does not interfere with their specific recognition of single bone proteins Italian Journal of Mineral and Electrolyte Metabolism, 1995
Osteocalcin induces chemotaxis, secretion of matrix proteins, and calcium-mediated intracellular signaling in human osteoclast-like cells Journal of Cell Biology, 1994
Human osteoclast-like cells recognize laminin via an RGD independent mechanism. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1993
Binding of osteopontin to the osteoclast integrin αvβ3 A. Miyauchi, J. Alvarez, E. M. Greenfield, A. Teti, M. Grano, S. Colucci, A. Zambonin-Zallone, F. P. Ross, S. L. Teitelbaum, D. Cheresh, K. A. Hruska Osteoporosis International, 1993
Osteoblastic control of osteoclast bone resorption in a serum-free co-culture system. Lack of effect of parathyroid hormone. Journal of Endocrinological Investigation, 1992
Cells from human bone giant cell tumors show a [Ca2+]o-sensing. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1992
Protein kinase C-dependent phosphorylation regulates osteoclast calcium-sensing. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1992
Integrin expression and adhesion property of osteoclast-like cells from giant cell tumours of bone. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1992
Functional and biochemical characterization of osteoclast-like cells derived from giant cell tumours of bone. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1992
Osteoblast-osteoclast interaction in bone resorption. Preliminary results. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1990
Osteoclast bone resorption is enhanced in the presence of osteoblasts. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1990
The role of protein kinase C in the osteoclast activity. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1990
Cytosolic free calcium dependent regulation of osteoclast bone resorbing activity. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1990
Voltage dependent calcium channel expression in isolated osteoclasts. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989
Immunolocalization of beta 3 subunit of integrins in osteoclast membrane. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989
Podosome expression in osteoclasts: influence of high extracellular calcium concentration. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989
Intracellular acidification induces decrease of cytosolic calcium in isolated osteoclasts. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989
Regulation of podosomes by intracellular pH in avian osteoclasts. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989
Cytoplasmic pH is regulated in isolated avian osteoclasts by a Cl-/HCO3- exchanger. Bollettino Della Societa Italiana Di Biologia Sperimentale, 1989