Marco Buscaglia

Scopus Publications

Polymer conformational entropy as the driver of complex coacervation
Tommaso Inzani, Giovanni Nava, Marco Buscaglia, Andrea Soranno, Alessandro Gori, et al.
Proceedings of the National Academy of Sciences of the United States of America, 2026
Understanding the mechanisms leading to complex coacervation is a fundamental challenge in biomolecular sciences. Here, we explored the condensation transition and pretransitional behavior of an archetypal system consisting of polyuridylic acid chains (PolyU) and short cationic peptides in buffered aqueous solutions. By combining static and dynamic light scattering with confocal microscopy, we determined the location of the transition and the partitioning of PolyU and peptides between the coexisting phases as a function of the total peptides/PolyU ratio. We find that upon adding peptides in the system, the size of PolyU coils in the single-phase region is progressively reduced by peptide-mediated intrachain bonds. Such conformational constraints become less severe in the dense phase, where part of the bonds become interchain as indicated by the sticky-reptation-type kinetics observed by dynamic light scattering. We propose a phenomenological model in which we include the loss in PolyU conformational entropy induced by peptide decoration and show that this quantity is large enough to be the main driver of the condensation transition. The model well reproduces the observed molecular partitioning between phases. We argue that the role of coil conformational relaxation, here demonstrated for a specific system, might be of general relevance in complex coacervation.
Sequence optimization of a DNA aptamer inhibiting COVID-19 infection guided by analysis of secondary structure distribution
Maria Izabel Muniz, Thomas Carzaniga, Giovanni Nava, Luca Casiraghi, Davide Giana, et al.
Computational and Structural Biotechnology Journal, 2026
Affinity-Based Copolymer Coating for Oriented Protein Immobilization in Biosensor Development
Lorenzo Zarini, Thomas Carzaniga, Morena Pirotta, Francesco Damin, Dario Brambilla, et al.
Biosensors, 2025
Effective protein immobilization is a critical step in biosensor development, as it ensures the stability, functionality, and orientation of biomolecules on the sensor surface. Here, we present a novel affinity-based terpolymer coating designed to enhance protein immobilization for biosensor applications. The novelty lies in the incorporation of nitrilotriacetic acid (NTA) ligands directly into the polymeric chains, facilitating histidine-tagged protein oriented binding through a robust metal-chelating interaction. To validate the system, magnetic microbeads coated with the polymer were tested for their ability to bind native and His-tagged proteins. The results demonstrated the superior binding capacity, enhanced stability, and reversibility of the interactions compared to traditional coatings, which immobilize proteins through nucleophile reactions with amine residues. Moreover, enzyme immobilization tests confirmed that the polymer preserves enzymatic activity, highlighting its potential for biosensor applications requiring functional biomolecules. This innovative polymeric coating offers a fast, versatile, and scalable solution for next-generation biosensor platforms, paving the way for improved sensitivity, reliability, and accessibility in diagnostic and analytical technologies.
Dynamics of Multisystem Inflammatory Syndrome in Children (MIS-C) associated to COVID-19: steady severity despite declining cases and new SARS-CoV-2 variants—a single-center cohort study
Thomas Carzaniga, Valeria Calcaterra, Luca Casiraghi, Tommaso Inzani, Stephana Carelli, et al.
European Journal of Pediatrics, 2025
Multisystem Inflammatory Syndrome in Children (MIS-C) is a serious condition associated with SARS-CoV-2 infection. The relationship between SARS-CoV-2 variants of concern (VOCs) and the occurrence and severity of MIS-C is unknown. We analyzed the dynamics of MIS-C in the Milan metropolitan area (Italy) during the COVID-19 pandemic, focusing on the epidemiologic trends and disease severity in relation to different VOCs in a single-center study. Fifty-seven MIS-C patients (mean 8.3 ± 3.8 years) admitted to the Pediatric Department of Buzzi Children’s Hospital in Milan, Italy, between November 2020 and July 2022, were retrospectively included in the study. The SARS-CoV-2 variant was retrospectively identified from serological fingerprinting (profiles of serum antibodies targeting different variants of SARS-CoV-2 obtained by a label-free microarray biosensor) or by the variant of prevalence. Two main periods of MIS-C case accumulation were observed. The peak of MIS-C cases rate in December 2020 reached 0.6 cases per day, which is nearly double the rate observed in February 2022, despite the larger number of infected subjects. Although the WT variant exhibited a broader range of severity score values, the score distributions for the different variants do not show statistically relevant differences. Conclusion: The results clearly show a decrease in the incidence of MIS-C in relation to infections, but also support the concept that severity of MIS-C remained essentially unchanged across different virus variants, including Omicron. The course of MIS-C, once initiated, is independent from the characteristics of the triggering variants, although later variants may be considered less likely to induce MIS-C. What is known:• MIS-C is a rare systemic inflammatory disorder that arises as a post-infectious complication temporally related to SARS-CoV-2 infection.• Fluctuations in MIS-C incidence were observed throughout the pandemic, with the latest variants associated with a lower incidence. What is new:• The SARS-CoV-2 variant of infection can be retrospectively confirmed by serum antibody fingerprinting using a label-free microarray biosensor.• Despite the decreasing incidence, MIS-C severity has remained essentially unchanged across SARS-CoV-2 variants.
Weak-cooperative binding of a long single-stranded DNA chain on a surface
Giovanni Nava, Thomas Carzaniga, Luca Casiraghi, Erik Bot, Giuliano Zanchetta, et al.
Nucleic Acids Research, 2024
Binding gene-wide single-stranded nucleic acids to surface-immobilized complementary probes is an important but challenging process for biophysical studies and diagnostic applications. The challenge comes from the conformational dynamics of the long chain that affects its accessibility and weakens its hybridization to the probes. We investigated the binding of bacteriophage genome M13mp18 on several different 20-mer probes immobilized on the surface of a multi-spot, label-free biosensor, and observed that only a few of them display strong binding capability with dissociation constant as low as 10 pM. Comparing experimental data and computational analysis of the M13mp18 chain structural features, we found that the capturing performance of a specific probe is directly related to the multiplicity of binding sites on the genomic strand, and poorly connected with the predicted secondary and tertiary structure. We show that a model of weak cooperativity of transient bonds is compatible with the measured binding kinetics and accounts for the enhancement of probe capturing observed when more than 20 partial pairings with binding free energy lower than -10 kcal mol−1 are present. This mechanism provides a specific pattern of response of a genomic strand on a panel of properly selected oligomer probe sequences.
Fetal Myocarditis Associated with Maternal SARS-CoV-2 Infection
Savina Mannarino, Mariano Lanna, Valeria Calcaterra, Thomas Carzaniga, Luca Casiraghi, et al.
Pediatric Infectious Disease Journal, 2024
We report the first case of significant fetal myocardial involvement associated with maternal SARS-CoV-2 infection, in which restoration of cardiac function at birth was noted. The demonstration of previous infection was supported by the quantification of humoral response in child and mother, in particular the presence of anti-N antibodies and through the detection of specific antibodies against the BA.4/5 variant.
Serum antibody fingerprinting of SARS-CoV-2 variants in infected and vaccinated subjects by label-free microarray biosensor
Thomas Carzaniga, Luca Casiraghi, Giovanni Nava, Giuliano Zanchetta, Tommaso Inzani, et al.
Frontiers in Immunology, 2024
Both viral infection and vaccination affect the antibody repertoire of a person. Here, we demonstrate that the analysis of serum antibodies generates information not only on the virus type that caused the infection but also on the specific virus variant. We developed a rapid multiplex assay providing a fingerprint of serum antibodies against five different SARS-CoV-2 variants based on a microarray of virus antigens immobilized on the surface of a label-free reflectometric biosensor. We analyzed serum from the plasma of convalescent subjects and vaccinated volunteers and extracted individual antibody profiles of both total immunoglobulin Ig and IgA fractions. We found that Ig level profiles were strongly correlated with the specific variant of infection or vaccination and that vaccinated subjects displayed a larger quantity of total Ig and a lower fraction of IgA relative to the population of convalescent unvaccinated subjects.
Digital Detection of Single Virus Particles by Multi-Spot, Label-Free Imaging Biosensor on Anti-Reflective Glass
Giovanni Nava, Luca Casiraghi, Thomas Carzaniga, Giuliano Zanchetta, Marcella Chiari, et al.
Small, 2023
Rapid detection of whole virus particles in biological or environmental samples represents an unmet need for the containment of infectious diseases. Here, an optical device enabling the enumeration of single virion particles binding on antibody or aptamers immobilized on a surface with anti‐reflective coating is described. In this regime, nanoparticles adhering to the sensor surface provide localized contributions to the reflected field that become detectable because of their mixing with the interfering waves in the reflection direction. Thus, these settings are exploited to realize a scan‐free, label‐free, micro‐array‐type digital assay on a disposable cartridge, in which the virion counting takes place in wide field‐of‐view imaging. With this approach we could quantify, by enumeration, different variants of SARS‐CoV‐2 virions interacting with antibodies and aptamers immobilized on different spots. For all tested variants, the aptamers showed larger affinity but lower specificity relative to the antibodies. It is found that the combination of different probes on the same surface enables increasing specificity of detection and dynamic range.
Invisible Fluorinated Materials for Optical Sensing
R Lanfranco, M Buscaglia
Encyclopedia of Materials Electronics, 2023
Label-free optical biosensors in the pandemic era
Giovanni Nava, Giuliano Zanchetta, Fabio Giavazzi, Marco Buscaglia
Nanophotonics, 2022
The research in the field of optical biosensors is continuously expanding, thanks both to the introduction of brand new technologies and the ingenious use of established methods. A new awareness on the potential societal impact of this research has arisen as a consequence of the Covid-19 pandemic. The availability of a new generation of analytical tools enabling a more accurate understanding of bio-molecular processes or the development of distributed diagnostic devices with improved performance is now in greater demand and more clearly envisioned, but not yet achieved. In this review, we focus on emerging innovation opportunities conveyed by label-free optical biosensors. We review the most recent innovations in label-free optical biosensor technology in consideration of their competitive potential in selected application areas. The operational simplicity implicit to label-free detection can be exploited in novel rapid and compact devices for distributed diagnostic applications. The adaptability to any molecular recognition or conformational process facilitates the integration of DNA nanostructures carrying novel functions. The high sensitivity to nanoscale objects stimulates the development of ultrasensitive systems down to digital detection of single molecular binding events enhanced by nanoparticles and direct enumeration of bio-nanoparticles like viruses.
Copolymer coatings for dna biosensors: Effect of charges and immobilization chemistries on yield, strength and kinetics of hybridization
Luka Vanjur, Thomas Carzaniga, Luca Casiraghi, Giuliano Zanchetta, Francesco Damin, et al.
Polymers, 2021
A bit stickier, a bit slower, a lot stiffer: Specific vs. nonspecific binding of gal4 to dna
Thomas Carzaniga, Giuliano Zanchetta, Elisa Frezza, Luca Casiraghi, Luka Vanjur, et al.
International Journal of Molecular Sciences, 2021
A microfluidic column of water index–matched packed microspheres for label-free observation of water pollutants
Roberta Lanfranco, Janire Saez, Deborah Abati, Thomas Carzaniga, Fernando Benito-Lopez, et al.
Microchimica Acta, 2021
Design of a rapid, multiplex, one-pot miRNA assay optimized by label-free analysis
G. Zanchetta, T. Carzaniga, L. Vanjur, L. Casiraghi, G. Tagliabue, et al.
Biosensors and Bioelectronics, 2021
Invisible Fluorinated Materials for Optical Sensing
Roberta Lanfranco, Marco Buscaglia
Encyclopedia of Smart Materials, 2021
Serological follow-up of SARS-CoV-2 asymptomatic subjects
Gregorio Paolo Milani, Laura Dioni, Chiara Favero, Laura Cantone, Chiara Macchi, et al.
Scientific Reports, 2020
Non-Langmuir Kinetics of DNA Surface Hybridization
Luka Vanjur, Thomas Carzaniga, Luca Casiraghi, Marcella Chiari, Giuliano Zanchetta, et al.
Biophysical Journal, 2020
SARS-CoV-2 infection among asymptomatic homebound subjects in Milan, Italy
Gregorio Paolo Milani, Emanuele Montomoli, Valentina Bollati, Benedetta Albetti, Claudio Bandi, et al.
European Journal of Internal Medicine, 2020
Fabrication and Optical Modeling of Micro-Porous Membranes Index-Matched with Water for On-Line Sensing Applications
Roberta Lanfranco, Fabio Giavazzi, Tommaso Bellini, Emanuele Di Nicolò, Marco Buscaglia
Macromolecular Materials and Engineering, 2020
Label-free optical sensing of pollutants in real water samples with a phantom membrane integrated into a microfluidic device
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2017, 2020
Multiplex, label-free quantification of miRNA by reflective phantom interface
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2019, 2019
Dynamics of Structural Elements of GB1 β-Hairpin Revealed by Tryptophan-Cysteine Contact Formation Experiments
Andrea Soranno, Francesca Cabassi, Maria Elena Orselli, Troy Cellmer, Alessandro Gori, et al.
Journal of Physical Chemistry B, 2018
Phantom membrane microfluidic cross-flow filtration device for the direct optical detection of water pollutants
R. Lanfranco, J. Saez, E. Di Nicolò, F. Benito-Lopez, M. Buscaglia
Sensors and Actuators B Chemical, 2018
Dark field differential dynamic microscopy enables accurate characterization of the roto-translational dynamics of bacteria and colloidal clusters
Roberto Cerbino, Davide Piotti, Marco Buscaglia, Fabio Giavazzi
Journal of Physics Condensed Matter, 2018
A label-free immunoassay for Flavivirus detection by the Reflective Phantom Interface technology
Giovanni Tagliabue, Valentina Faoro, Serena Rizzo, Daniele Sblattero, Andrea Saccani, et al.
Biochemical and Biophysical Research Communications, 2017
Emerging applications of label-free optical biosensors
Giuliano Zanchetta, Roberta Lanfranco, Fabio Giavazzi, Tommaso Bellini, Marco Buscaglia
Nanophotonics, 2017
Selective Adsorption on Fluorinated Plastic Enables the Optical Detection of Molecular Pollutants in Water
R. Lanfranco, F. Giavazzi, M. Salina, G. Tagliabue, E. Di Nicolò, et al.
Physical Review Applied, 2016
Multi-spot, label-free detection of viral infection in complex media by a non-reflecting surface
Matteo Salina, Fabio Giavazzi, Erica Ceccarello, Francesco Damin, Marcella Chiari, et al.
Sensors and Actuators B Chemical, 2016
Phantom microbeads chromatography column in microfluidics
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2016, 2016
Label-free detection of DNA single-base mismatches using a simple reflectance-based optical technique
G. Nava, E. Ceccarello, F. Giavazzi, M. Salina, F. Damin, et al.
Physical Chemistry Chemical Physics, 2016
Label-free optical detection of molecular and cellular binding on reflectionless glass
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences Microtas 2016, 2016
Multi-spot, label-free immunoassay on reflectionless glass
Matteo Salina, Fabio Giavazzi, Roberta Lanfranco, Erica Ceccarello, Laura Sola, et al.
Biosensors and Bioelectronics, 2015
New "clickable" polymeric coating for glycan microarrays
Caterina Zilio, Anna Bernardi, Alessandro Palmioli, Matteo Salina, Giovanni Tagliabue, et al.
Sensors and Actuators B Chemical, 2015
Optical detection of surfactants by means of reflective phantom interface method
R. Lanfranco, F. Giavazzi, M. Salina, E. Di Nicolò, M. Buscaglia
Lecture Notes in Electrical Engineering, 2015
Portable, multispot, label-free immunoassay on a phantom perfluorinated plastic
Fabio Giavazzi, Matteo Salina, Erica Ceccarello, Mattia Bassi, Francesco Damin, et al.
Lecture Notes in Electrical Engineering, 2015
A fast and simple label-free immunoassay based on a smartphone
Fabio Giavazzi, Matteo Salina, Erica Ceccarello, Andrea Ilacqua, Francesco Damin, et al.
Biosensors and Bioelectronics, 2014
Viscoelasticity of nematic liquid crystals at a glance
Fabio Giavazzi, Stefano Crotti, Antonio Speciale, Francesca Serra, Giuliano Zanchetta, et al.
Soft Matter, 2014
Multi-spot, label-free detection of biomarkers in complex media by reflectionless surfaces
M. Salina, F. Giavazzi, E. Ceccarello, F. Damin, M. Chiari, et al.
Procedia Engineering, 2014
Nematic liquid crystals embedded in cubic microlattices: Memory effects and bistable pixels
Francesca Serra, Shane Michael Eaton, Roberto Cerbino, Marco Buscaglia, Giulio Cerullo, et al.
Advanced Functional Materials, 2013
Multispot, label-free biodetection at a phantom plastic-water interface
Fabio Giavazzi, Matteo Salina, Roberto Cerbino, Mattia Bassi, Davide Prosperi, et al.
Proceedings of the National Academy of Sciences of the United States of America, 2013
Bistability of nematic liquid crystals confined in 3D scaffold produced by two-photon polymerization
2012 Conference on Lasers and Electro Optics CLEO 2012, 2012
Effect of configuration of the microchannels fabricated by femtosecond laser micromachining on topological defects in confined liquid crystals
Krishna Chaitanya Vishnubhatla, Roberto Osellame, Giulio Cerullo, Francesca Serra, Roberto Cerbino, et al.
Proceedings of SPIE the International Society for Optical Engineering, 2012
Bistability of nematic liquid crystals confined in 3D scaffold produced by two-photon polymerization
Francesca Serra, Shane M. Eaton, Eleon Borlini, Roberto Cerbino, Marco Buscaglia, et al.
CLEO Science and Innovations CLEO Si 2012, 2012
Topological defects of nematic liquid crystals confined in porous networks
Francesca Serra, Krishna C. Vishnubhatla, Marco Buscaglia, Roberto Cerbino, Roberto Osellame, et al.
Soft Matter, 2011
Making connections between ultrafast protein folding kinetics and molecular dynamics simulations
Troy Cellmer, Marco Buscaglia, Eric R. Henry, James Hofrichter, William A. Eaton
Proceedings of the National Academy of Sciences of the United States of America, 2011
The emergence of memory in liquid crystals
Francesca Serra, Marco Buscaglia, Tommaso Bellini
Materials Today, 2011
Memory and topological frustration in nematic liquid crystals confined in porous materials
Takeaki Araki, Marco Buscaglia, Tommaso Bellini, Hajime Tanaka
Nature Materials, 2011
Towards a universal method for the stable and clean functionalization of inert perfluoropolymer nanoparticles: Exploiting photopolymerizable amphiphilic diacetylenes
Carlo Morasso, Miriam Colombo, Silvia Ronchi, Laura Polito, Serena Mazzucchelli, et al.
Advanced Functional Materials, 2010
Elastic anisotropy at a glance: The optical signature of disclination lines
Marco Buscaglia, Giuseppe Lombardo, Lorenzo Cavalli, Riccardo Barberi, Tommaso Bellini
Soft Matter, 2010
Right-handed double-helix ultrashort DNA yields chiral nematic phases with both right- and left-handed director twist
Giuliano Zanchetta, Fabio Giavazzi, Michi Nakata, Marco Buscaglia, Roberto Cerbino, et al.
Proceedings of the National Academy of Sciences of the United States of America, 2010
Field-induced clearing in sphere-sphere and rod-sphere binary mixtures of charged colloidal particles
L. Fornasari, F. Mantegazza, M. L. Jimenez, M. Buscaglia, T. Bellini
Physical Review E Statistical Nonlinear and Soft Matter Physics, 2009
Kinetics of contact formation and end-to-end distance distributions of swollen disordered peptides
Andrea Soranno, Renato Longhi, Tommaso Bellini, Marco Buscaglia
Biophysical Journal, 2009
Liquid crystal ordering of DNA and RNA oligomers with partially overlapping sequences
G Zanchetta, M Nakata, M Buscaglia, N A Clark, T Bellini
Journal of Physics Condensed Matter, 2008
Liquid crystal alignment on a chiral surface: Interfacial interaction with sheared DNA films
M. Nakata, G. Zanchetta, M. Buscaglia, T. Bellini, N. A. Clark
Langmuir, 2008
Phase separation and liquid crystallization of complementary sequences in mixtures of nanoDNA oligomers
Giuliano Zanchetta, Michi Nakata, Marco Buscaglia, Tommaso Bellini, Noel A. Clark
Proceedings of the National Academy of Sciences of the United States of America, 2008
Phantom nanoparticles as probes of biomolecular interactions
Davide Prosperi, Carlo Morasso, Francesco Mantegazza, Marco Buscaglia, Loren Hough, et al.
Small, 2006
Memory effects in nematics with quenched disorder
M. Buscaglia, T. Bellini, C. Chiccoli, F. Mantegazza, P. Pasini, et al.
Physical Review E Statistical Nonlinear and Soft Matter Physics, 2006
Effects of denaturants on the dynamics of loop formation in polypeptides
Marco Buscaglia, Lisa J. Lapidus, William A. Eaton, James Hofrichter
Biophysical Journal, 2006
Determination of ultrafast protein folding rates from loop formation dynamics
Marco Buscaglia, Jan Kubelka, William A. Eaton, James Hofrichter
Journal of Molecular Biology, 2005
Nematics with quenched disorder: Pinning out the origin of memory
M. Rotunno, M. Buscaglia, C. Chiccoli, F. Mantegazza, P. Pasini, et al.
Physical Review Letters, 2005
Guided propagation in electric-field-controlled hybrid nematic waveguides
M. Marangoni, R. Osellame, R. Ramponi, M. Buscaglia, T. Bellini
Journal of Applied Physics, 2004
New computer model of a dipolar system with hexagonal geometry in presence of an external electric field
Rafaella Turri, M. Buscaglia, Giovanni Danese, Ivo De Lotto, Francesco Leporati
Lecture Notes in Computer Science Including Subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics, 2004
Reshaping of the refractive index profile of a liquid crystal waveguide by means of an external electric field
Roberta Ramponi, Roberto Osellame, Marco Marangoni, Marco Buscaglia, Tommaso Bellini
Proceedings of SPIE the International Society for Optical Engineering, 2003
Dynamic control of the optical properties of a liquid crystal waveguide by means of an applied electric field
Marco Marangoni, Marco Buscaglia, Roberto Osellame, Francesco Mantegazza, Tommaso Bellini, et al.
Proceedings of SPIE the International Society for Optical Engineering, 2003
Kinetics of intramolecular contact formation in a denatured protein
Marco Buscaglia, Benjamin Schuler, Lisa J. Lapidus, William A. Eaton, James Hofrichter
Journal of Molecular Biology, 2003
Field-controlled optical profile of a waveguide having a liquid-crystalline core
M. Marangoni, R. Osellame, R. Ramponi, M. Buscaglia, T. Bellini, et al.
Applied Physics Letters, 2002
Nematics with quenched disorder: How long will it take to heal?
T. Bellini, M. Buscaglia, C. Chiccoli, F. Mantegazza, P. Pasini, et al.
Physical Review Letters, 2002
Field-induced anti-nematic ordering in assemblies of anisotropically polarizable particles
T Bellini, M Buscaglia, F Leporati, F Mantegazza, A Maritan
Europhysics Letters, 2001
A laser interferometer for the study of electrically excited bubble capillary modes
T. Bellini, M. Buscaglia, M. Corti, F. Mantegazza, C. Rocca
Colloids and Surfaces A Physicochemical and Engineering Aspects, 2001
Monte Carlo Metropolis simulation of interacting anisotropic polarizable spins on a lattice
G. Danese, I. De Lotto, A. De Marchi, F. Leporati, T. Bellini, et al.
Computer Physics Communications, 2001
Electrokinetic properties of colloids of variable charge. III. Observation of a Maxwell-Wagner relaxation mechanism by high-frequency electric-birefringence spectroscopy
F. Mantegazza, T. Bellini, M. Buscaglia, V. Degiorgio, D. A. Saville
Journal of Chemical Physics, 2000
Nematics with quenched disorder: what is left when long range order is disrupted?
T. Bellini, M. Buscaglia, C. Chiccoli, F. Mantegazza, P. Pasini, et al.
Physical Review Letters, 2000
Non-linear dynamics of the electro-opticresponse of confined liquid crystals
M Buscaglia, T Bellini, V Degiorgio, F Mantegazza, F Simoni
Europhysics Letters, 1999

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