Arun Kumar Parthasarathy

@dypatilunikop.org

Assistant Lecturer, Microbiology
D.Y Patil Medical College, Kolhapur

Arun Kumar Parthasarathy


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https://researchid.co/microarun

EDUCATION

M.Sc Medical Microbiology, Ph.D Medical Microbiology

RESEARCH INTERESTS

Infectious disease, Antimicrobial Resistance , Environmental and Agricultural Microbiology
6

Scopus Publications

72

Scholar Citations

5

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • DEVELOPMENT OF REDUCED GRAPHENE OXIDE (RGO) NANOSHEETS FOR ULTRASENSITIVE DETECTION OF A NOVEL CORONA VIRUS SARS-COV-2
    DEEPAK V. SAWANT, PRANAV K. KATKAR, ARUNKUMAR PARTHASARATHY, R. K. SHARMA, SHIVAJI KASHTE, C. D. LOKHANDE
    International Journal of Applied Pharmaceutics, 2025
    Objective: The extraction of ribonucleic acid (RNA) is essential for SARS-CoV-2 RT-PCR detection. Although commercial RNA extraction kits are available, they suffer from limitations such as low RNA yield, difficulty in amplification during RT-PCR, and high costs. This study investigates the evolution of rGO Nano sheets as a novel material for efficient RNA extraction from clinical specimens. The rGO Nano sheets offer a promising alternative for the purification of excellent RNA, which is crucial for accurate and high-sensitivity identification of SARS-CoV-2. By leveraging a unique property of rGO, the research demonstrates enhanced RNA recovery, improving the overall sensitivity and reliability of detection method for SARS-CoV-2. Methods: The rGO Nano sheets were synthesized by reducing graphene oxide (GO) using a simple hydrothermal process. The rGO analysis and its interaction with RNA were characterized using X-ray diffraction, Raman spectroscopy, field emission scanning electron microscopy (FE-SEM), and Brunauer-Emmett-Teller (BET) analysis. RNA was extracted from clinical specimens using rGO Nano sheets via adsorption and elution and compared with extraction performed using a commercial RNA kit. RNA extraction efficiency and validation were assessed by UV-visible spectrometry and RT-PCR. Results: The rGO Nano sheets exhibited a multi-layered carbon nanostructure with an average 7.15 nm 3D porous structure, a large surface area of 225 m²/g, and interconnected carbon Nano sheets. The RNA extracted using rGO Nano sheets was validated by UV-visible spectrometry and RT-PCR, showing successful detection of viral genes (ORF1ab and N gene) with a sensitivity of 10 copies. Ten to twenty copies of SARS-CoV-2 pseudo-virus particles showed a strong linear association. The P-value is greater than 0.05, indicating acceptance of the null hypothesis. The commercial kit and the rGO Nano sheet-based RNA extraction methods are not significantly different from one another. There is a significant increase in RNA yield (1.959 ng/µl) and improved sensitivity in RT-PCR, further validating the advantage of using rGO for RNA extraction. Conclusion: The developed rGO Nano sheet-based RNA extraction method demonstrates high sensitivity and efficiency, offering a promising alternative to commercial kits for the detection of SARS-CoV-2.
  • Optical Biosensing of SARS-CoV-2 RNA Based on Positively Charged Poly-l-Lysine Functionalized Gold Nanoparticles
    Tejaswini P. Patil, Arun Kumar Parthasarathy, Dhanaji Malavekar, JinHyeok Kim, Arpita P. Tiwari
    Journal of Cluster Science, 2024
  • Environmental pollutants and their impact on COVID-19 spread: Current problem and future resolutions
    Pooja M. Patil, Arun K. Parthasarathy, Abhijeet R. Matkar, Pranjali Mahamuni‐Badiger, Maruti J. Dhanavade
    Carbon Neutralization, 2023
    COVID‐19 is the greatest crucial universal health issue of the century and the extreme challenge that came after the 2nd World War faced by humankind. In 2019, different strains of the coronavirus have emerged drastically, that as severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) which is the causative agent of corona disease. As far as human civilization history, there have been occurrences of severe diseases and outbreaks of various viruses. According to World Health Organization reports, throughout the world, the present outbreak of COVID‐19 has engulfed more than 200 countries affecting 241,471,559 individuals and more than 4,914,092 people lost their lives. SARS‐CoV‐2 outbreak is severely disturbing the worldwide economy at present. The urgent need of recent times is to understand the environmental aspect of COVID‐19 disease. Hence to fulfill this point, the present review article is compiled with a brief discussion about all the minute points of the COVID‐19 pandemic related to the environment: origin and present scenario, the occurrence of SARS‐CoV‐2 in environmental habitats, the effect of COVID‐19 on human health, and environment, environmental factors influencing the transmission and spreading of SARS‐CoV‐2. This review explains micro and macro pollutants in hospital and urban wastewater influencing COVID‐19, detection of SARS‐CoV‐2, current global drug strategies to control replication and spread of SARS‐CoV‐2 from the environment, future approaches, and guidelines to prevent and control upcoming pandemics. The SARS‐CoV‐2 structural details and their effect on humans have been already well presented but the research about environmental factors affecting COVID‐19 could be important points to fight present and future pandemics.
  • pH-responsive glycine functionalized magnetic iron oxide nanoparticles for SARS-CoV-2 RNA extraction from clinical sample
    Rutuja Prashant Gambhir, Arun Kumar Parthasarathy, Shimpa Sharma, Shital Kale, Vikas Vijay Magdum, Arpita Pandey Tiwari
    Journal of Materials Science, 2022
  • Prevalence of Methicillin Resistant Staphylococcus aureus and its Associated SCCmec Types among Healthcare workers and Patient Visitors from Western Maharashtra, India
    Arun Kumar Parthasarathy, Dinesh Babu R, Roma A. Chougale
    Journal of Pure and Applied Microbiology, 2022
    Staphylococcus aureus is one of the major pathogen causing infections in human ranging from mild to severe life-threatening conditions. Methicillin-Resistant Staphylococcus aureus (MRSA) is an important nosocomial pathogen with high morbidity and mortality in both hospital and community settings. Total 600 nasal swabs were collected from patient visitors and Healthcare workers. Of these, 184 S.aureus (30.66%) were isolated. All S.aureus isolates screened for MRSA and 73 (39.67%) isolates showed MRSA by Cefoxitin disc diffusion method and PCR. 21 (28.76%) isolates detected pvl gene of the 73 isolated MRSA i.e., CA-MRSA. All MRSA isolates were typed into SCCmec element (I to V). Of these SCCmec type III was found more prevalent than other SCCmec types and 3 isolates were not typeable. MRSA still remains a significant problem in public Healthcare settings. Screening of MRSA among Healthcare Workers and patient visitors is mandatory to prevent the spread of CA-MRSA in hospitals.
  • Bacterial Contamination of White Coats among Medical Personnel- A Cross Sectional Study in Kolhapur, India
    P. Arun Kumar, Roma A. Chougale, Indireddy Sinduri
    Journal of Pure and Applied Microbiology, 2020
    To minimize exposure to hazardous biological materials, all doctors, nurses, technicians and in general all the healthcare personnel as well as researchers wear a knee-length, long-sleeved, elastic-cuffed laboratory coat while working with hazardous materials. A white coat provides a protective layer and can be easily removed if contaminated. White coats act as mechanical vectors in transmission of pathogenic and non-pathogenic bacteria to the people who are associated with health care workers. A total of 120 specimens were collected by using sterile cotton swabs. Of these, 88 (73%) white coats were contaminated. At the same time, a pre-validated and semi-structured questionnaire (containing questions regarding usage of white coat, purpose, hygiene, and perception about contamination of white coats, etc) was distributed. Gram positive cocci 64 (72.72%) were isolated more than gram negative bacilli 24 (27.28%). Most of the tested antibiotics showed resistance to isolated gram positive and gram negative bacteria. 10 (41.66%) isolates of gram negative bacilli were resistant to extended spectrum beta-lactamases (ESBL) and 10 (6.4%) of staphylococcal isolates showed Methicillin resistance. Therefore, a much more attention to wear a clean white coat should be required.

RECENT SCHOLAR PUBLICATIONS

  • DEVELOPMENT OF REDUCED GRAPHENE OXIDE (RGO) NANOSHEETS FOR ULTRASENSITIVE DETECTION OF A NOVEL CORONA VIRUS SARS-COV-2
    DV SAWANT, PK KATKAR, A PARTHASARATHY, RK SHARMA, ...
    Int J App Pharm 17 (5), 263-270 , 2025
    2025
  • Molecular Characterization of Adhesions and Fimbriae in Proteus Mirabilis Associated with Urinary Tract Colonization : A Genomic Approach
    PAK S. Jayapradha,T.Jeyaseelan senthinath
    African Journal of Biomedical Research 27 (4S), 1538 -1543 , 2024
    2024
  • Optical Biosensing of SARS-CoV-2 RNA Based on Positively Charged Poly- l -Lysine Functionalized Gold Nanoparticles
    TP Patil, AK Parthasarathy, D Malavekar, JH Kim, AP Tiwari
    Journal of Cluster Science 35 (7), 2525-2538 , 2024
    2024
    Citations: 4
  • Nitrite-Oxidizing Bacteria: Cultivation, Growth Physiology, and Chemotaxonomy
    PM Patil, AK Parthasarathy, AR Matkar, P Mahamuni-Badiger, S Hwang, ...
    2023
    Citations: 5
  • Comparison of dexmedetomidine-fentanyl and dexmedetomidine-pentazocine for monitored anesthesia care during tympanoplasty surgery
    AA Chougale, AK Parthasarathy, V Dhulkhed, R Chougale
    Int. J Med Anaesthesiol 6, 44-50 , 2023
    2023
    Citations: 1
  • Environmental pollutants and their impact on COVID‐19 spread: Current problem and future resolutions
    PM Patil, AK Parthasarathy, AR Matkar, P Mahamuni‐Badiger, ...
    Carbon Neutralization 2 (1), 127-146 , 2023
    2023
    Citations: 2
  • Isatin inhibits adhesion and Biofilm formation in Candida albicans Isatin as anti-Candida albicans agent
    AK Jadhav, SA Chaugule, AK Parthasarathy, D Sawant, SM Karuppayil
    2022
  • pH-responsive glycine functionalized magnetic iron oxide nanoparticles for SARS-CoV-2 RNA extraction from clinical sample
    RP Gambhir, AK Parthasarathy, S Sharma, S Kale, VV Magdum, ...
    Journal of materials science 57 (28), 13620-13631 , 2022
    2022
    Citations: 18
  • Prevalence of methicillin resistant Staphylococcus aureus and its associated sccmec types among healthcare workers and patient visitors from western Maharashtra, India.
    AK Parthasarathy, RA Chougale
    Journal of Pure & Applied Microbiology 16 (2) , 2022
    2022
    Citations: 6
  • Prevalence of Erythromycin and Tetracycline resistance determinants among Coagulase Negative Staphylococcus of Healthcare Workers
    AK Parthasarathy, RA Chougale, P Shadija
    NOVYI MIR Research Journal 7 (5), 30 - 40 , 2022
    2022
  • Antibiotic Resistant Staphylococcus
    AK Parthasarathy, RA Chougale
    Insights into Drug Resistance in Staphylococcus aureus, 3 , 2021
    2021
    Citations: 5
  • Kocuria kristinae (unusual pathogen) isolated from cerebrospinal fluid: a case of acute meningitis
    PA Kumar, RA Chougale
    Highlights on medicine and medical research 15, 93-97 , 2021
    2021
    Citations: 1
  • Bacterial contamination of white coats among medical personnel-a cross sectional study in Kolhapur, India
    PA Kumar, RA Chougale, I Sinduri
    Journal of Pure and Applied Microbiology 14 (2), 1405-1411 , 2020
    2020
    Citations: 19
  • Hand-Hygiene awareness and Compliance from a tertiary care teaching hospital, Kolhapur
    A Kumar, RA Chougale
    JMSCR 7, 1045-1053 , 2019
    2019
    Citations: 1
  • Evaluate the Awareness of Hand washing by using Semi- Structured Questionnaire from a tertiary care hospital, Kolhapur
    AC Roma, KP Arun
    International Journal of Research and Analytical Reviews 1 (Special issue … , 2019
    2019
  • Aerobic microbiological surveillance of operation theatre from a tertiary care hospital, Kolhapur, India
    RA Chougale, PA Kumar
    Int J Curr Microbiol App Sci 8 (10), 1210-1215 , 2019
    2019
    Citations: 3
  • Unusual Pathogen-Kocuria kristinae isolated from Cerebrospinal fluid, a case of acute meningitis
    A Kumar, RA Chougale
    Journal of Medical Science and Clinical Research (JMSCR) 6 (7), 1012-1015 , 2018
    2018
    Citations: 1
  • Bacteriological profile of Blood Stream Infection in Tertiary care Hospital, Kolhapur
    A Kumar, RA Chougale, M Pandian
    IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 17 (10), 54-58 , 2018
    2018
  • Rapid Detection of Mycobacterium tuberculosis by using MPT64 Immunochromatographic Assay
    R Dinesh Babu, P Arun Kumar, HK Purohit, C Shivashri
    J Med Microb Diagn 7 (282), 2161-0703.1000282 , 2018
    2018
    Citations: 6

MOST CITED SCHOLAR PUBLICATIONS

  • Bacterial contamination of white coats among medical personnel-a cross sectional study in Kolhapur, India
    PA Kumar, RA Chougale, I Sinduri
    Journal of Pure and Applied Microbiology 14 (2), 1405-1411 , 2020
    2020
    Citations: 19
  • pH-responsive glycine functionalized magnetic iron oxide nanoparticles for SARS-CoV-2 RNA extraction from clinical sample
    RP Gambhir, AK Parthasarathy, S Sharma, S Kale, VV Magdum, ...
    Journal of materials science 57 (28), 13620-13631 , 2022
    2022
    Citations: 18
  • Prevalence of methicillin resistant Staphylococcus aureus and its associated sccmec types among healthcare workers and patient visitors from western Maharashtra, India.
    AK Parthasarathy, RA Chougale
    Journal of Pure & Applied Microbiology 16 (2) , 2022
    2022
    Citations: 6
  • Rapid Detection of Mycobacterium tuberculosis by using MPT64 Immunochromatographic Assay
    R Dinesh Babu, P Arun Kumar, HK Purohit, C Shivashri
    J Med Microb Diagn 7 (282), 2161-0703.1000282 , 2018
    2018
    Citations: 6
  • Nitrite-Oxidizing Bacteria: Cultivation, Growth Physiology, and Chemotaxonomy
    PM Patil, AK Parthasarathy, AR Matkar, P Mahamuni-Badiger, S Hwang, ...
    2023
    Citations: 5
  • Antibiotic Resistant Staphylococcus
    AK Parthasarathy, RA Chougale
    Insights into Drug Resistance in Staphylococcus aureus, 3 , 2021
    2021
    Citations: 5
  • Optical Biosensing of SARS-CoV-2 RNA Based on Positively Charged Poly- l -Lysine Functionalized Gold Nanoparticles
    TP Patil, AK Parthasarathy, D Malavekar, JH Kim, AP Tiwari
    Journal of Cluster Science 35 (7), 2525-2538 , 2024
    2024
    Citations: 4
  • Aerobic microbiological surveillance of operation theatre from a tertiary care hospital, Kolhapur, India
    RA Chougale, PA Kumar
    Int J Curr Microbiol App Sci 8 (10), 1210-1215 , 2019
    2019
    Citations: 3
  • Environmental pollutants and their impact on COVID‐19 spread: Current problem and future resolutions
    PM Patil, AK Parthasarathy, AR Matkar, P Mahamuni‐Badiger, ...
    Carbon Neutralization 2 (1), 127-146 , 2023
    2023
    Citations: 2
  • Comparison of dexmedetomidine-fentanyl and dexmedetomidine-pentazocine for monitored anesthesia care during tympanoplasty surgery
    AA Chougale, AK Parthasarathy, V Dhulkhed, R Chougale
    Int. J Med Anaesthesiol 6, 44-50 , 2023
    2023
    Citations: 1
  • Kocuria kristinae (unusual pathogen) isolated from cerebrospinal fluid: a case of acute meningitis
    PA Kumar, RA Chougale
    Highlights on medicine and medical research 15, 93-97 , 2021
    2021
    Citations: 1
  • Hand-Hygiene awareness and Compliance from a tertiary care teaching hospital, Kolhapur
    A Kumar, RA Chougale
    JMSCR 7, 1045-1053 , 2019
    2019
    Citations: 1
  • Unusual Pathogen-Kocuria kristinae isolated from Cerebrospinal fluid, a case of acute meningitis
    A Kumar, RA Chougale
    Journal of Medical Science and Clinical Research (JMSCR) 6 (7), 1012-1015 , 2018
    2018
    Citations: 1
  • DEVELOPMENT OF REDUCED GRAPHENE OXIDE (RGO) NANOSHEETS FOR ULTRASENSITIVE DETECTION OF A NOVEL CORONA VIRUS SARS-COV-2
    DV SAWANT, PK KATKAR, A PARTHASARATHY, RK SHARMA, ...
    Int J App Pharm 17 (5), 263-270 , 2025
    2025
  • Molecular Characterization of Adhesions and Fimbriae in Proteus Mirabilis Associated with Urinary Tract Colonization : A Genomic Approach
    PAK S. Jayapradha,T.Jeyaseelan senthinath
    African Journal of Biomedical Research 27 (4S), 1538 -1543 , 2024
    2024
  • Isatin inhibits adhesion and Biofilm formation in Candida albicans Isatin as anti-Candida albicans agent
    AK Jadhav, SA Chaugule, AK Parthasarathy, D Sawant, SM Karuppayil
    2022
  • Prevalence of Erythromycin and Tetracycline resistance determinants among Coagulase Negative Staphylococcus of Healthcare Workers
    AK Parthasarathy, RA Chougale, P Shadija
    NOVYI MIR Research Journal 7 (5), 30 - 40 , 2022
    2022
  • Evaluate the Awareness of Hand washing by using Semi- Structured Questionnaire from a tertiary care hospital, Kolhapur
    AC Roma, KP Arun
    International Journal of Research and Analytical Reviews 1 (Special issue … , 2019
    2019
  • Bacteriological profile of Blood Stream Infection in Tertiary care Hospital, Kolhapur
    A Kumar, RA Chougale, M Pandian
    IOSR Journal of Dental and Medical Sciences (IOSR-JDMS) 17 (10), 54-58 , 2018
    2018