Arun Kumar Parthasarathy
@dypatilunikop.org
Assistant Lecturer, Microbiology
D.Y Patil Medical College, Kolhapur
EDUCATION
M.Sc Medical Microbiology, Ph.D Medical Microbiology
RESEARCH INTERESTS
Infectious disease, Antimicrobial Resistance , Environmental and Agricultural Microbiology
Scopus Publications
Scholar Citations
Scholar h-index
Scopus Publications
Rutuja Prashant Gambhir, Arun Kumar Parthasarathy, Shimpa Sharma, Shital Kale, Vikas Vijay Magdum, and Arpita Pandey Tiwari
Springer Science and Business Media LLC
Arun Kumar Parthasarathy, Dinesh Babu R, and Roma A. Chougale
Journal of Pure and Applied Microbiology
Staphylococcus aureus is one of the major pathogen causing infections in human ranging from mild to severe life-threatening conditions. Methicillin-Resistant Staphylococcus aureus (MRSA) is an important nosocomial pathogen with high morbidity and mortality in both hospital and community settings. Total 600 nasal swabs were collected from patient visitors and Healthcare workers. Of these, 184 S.aureus (30.66%) were isolated. All S.aureus isolates screened for MRSA and 73 (39.67%) isolates showed MRSA by Cefoxitin disc diffusion method and PCR. 21 (28.76%) isolates detected pvl gene of the 73 isolated MRSA i.e., CA-MRSA. All MRSA isolates were typed into SCCmec element (I to V). Of these SCCmec type III was found more prevalent than other SCCmec types and 3 isolates were not typeable. MRSA still remains a significant problem in public Healthcare settings. Screening of MRSA among Healthcare Workers and patient visitors is mandatory to prevent the spread of CA-MRSA in hospitals.
P. Arun Kumar, Roma A. Chougale, and Indireddy Sinduri
Journal of Pure and Applied Microbiology
To minimize exposure to hazardous biological materials, all doctors, nurses, technicians and in general all the healthcare personnel as well as researchers wear a knee-length, long-sleeved, elastic-cuffed laboratory coat while working with hazardous materials. A white coat provides a protective layer and can be easily removed if contaminated. White coats act as mechanical vectors in transmission of pathogenic and non-pathogenic bacteria to the people who are associated with health care workers. A total of 120 specimens were collected by using sterile cotton swabs. Of these, 88 (73%) white coats were contaminated. At the same time, a pre-validated and semi-structured questionnaire (containing questions regarding usage of white coat, purpose, hygiene, and perception about contamination of white coats, etc) was distributed. Gram positive cocci 64 (72.72%) were isolated more than gram negative bacilli 24 (27.28%). Most of the tested antibiotics showed resistance to isolated gram positive and gram negative bacteria. 10 (41.66%) isolates of gram negative bacilli were resistant to extended spectrum beta-lactamases (ESBL) and 10 (6.4%) of staphylococcal isolates showed Methicillin resistance. Therefore, a much more attention to wear a clean white coat should be required.