Emilia Angela Lo Schiavo Arisawa
@univap.br
Development and Research Institute
Universidade do Vale do Paraíba
RESEARCH, TEACHING, or OTHER INTERESTS
Pathology and Forensic Medicine, Research and Theory, Multidisciplinary
Scopus Publications
Scopus Publications
Fernanda Cláudia Miranda Amorim, Emilia Ângela Loschiavo Arisawa, Luciana Barros Sant’anna, Ana Beatriz Mendes Rodrigues, and Davidson Ribeiro Costa
FapUNIFESP (SciELO)
Abstract Objective: to evaluate the effect of photobiomodulation with low-level 660 nm laser alone or associated with Human Amniotic Membrane in the repair of partial-thickness burns in rats. Method: an experimental study conducted with 48 male Wistar rats, randomized into four groups: Control, Human Amniotic Membrane, Low-Level Laser Therapy, and Low-Level Laser Therapy associated with Human Amniotic Membrane. The histopathological characteristics of the skin samples were analyzed 7 and 14 days after the burn. The data obtained were submitted to the Kolmogorov-Smirnov and Mann-Whitney tests. Results: the histological analysis of the burn injuries showed a decrease in inflammation (p<0.0001) and an increase in proliferation of fibroblasts (p<0.0001) mainly at 7 days in all treatments related to the control group. At 14 days, the greater effectiveness in accelerating the healing process was significant (p<0.0001) in the Low-Level Laser Therapy group associated with the Human Amniotic Membrane. Conclusion: the association of photobiomodulation therapies with the Human Amniotic Membrane allowed verifying a reduction in the healing process time of the experimental lesions, stimulating its proposal as a treatment protocol in partial-thickness burns.
Rodrigo Alvaro B. Lopes-Martins, Douglas Peretta Barbaroto, Elaine Da Silva Barbosa, Patrícia Sardinha Leonardo, Carlos Ruiz-Silva, and Emília Angela Lo Schiavo Arisawa
Springer Science and Business Media LLC
Elisabeth Salmagi Teixeira Coutinho, Lázaro Pinto Medeiros Neto, Tanmoy Bhattacharjee, Emilia Angela Lo Schiavo Arisawa, and Luciana Barros Sant'Anna
Elsevier BV
Joelita de Alencar F. Santos, Renata Nicolau, Luciana Sant’Anna, Josne Paterno, Priscila Cristovam, Jose Gomes, José Santos, and Emilia Arisawa
HMP Communications, LLC
BACKGROUND: Low-level laser therapy (LLLT) and human amniotic membrane (HAM) application have been shown to be viable options for use in wound healing. PURPOSE: This study sought to compare LLLT and HAM to a control treatment (hydrogel, saline, and gauze) in persons with diabetes mellitus (DM) and foot ulcers. METHODS: Using a prospective pilot clinical study design, patients receiving care at a health center that specializes in the treatment of diabetic foot wounds between November 2016 and August 2017 were recruited. Eligible patients had to be 30 to 59 years of age; diagnosed with type 2 DM (postprandial capillary glucose levels between 140 and 350 mg/dL); and have uninfected, granulating stage 2 or 3 foot ulcers measuring less than 7 cm by 3 cm. Immunosuppressed and malnourished patients or those with neoplasms or in critical condition were not eligible to participate. Patients received the control treatment (2 mg hydrogel, saline, and gauze), HAM (patches of thawed HAM, applied with overlapping edges), or LLLT (phototherapy session, 2 mg hydrogel, saline, and gauze) for 28 days. Variables, wound area measurements, Pressure Ulcer Scale for Healing (PUSH) scores, and Visual Analog Scale (VAS) scores were used to assess wound improvement progress and pain on days 7, 14, 21, and 28. Descriptive statistics were used to analyze the participant anthropometric and clinical profiles. The Kolmogorov-Smirnov test was used to analyze the sample distribution. The Kruskal-Wallis test with Dunn’s post-test was used to evaluate differences in PUSH and VAS scores and wound size for intergroup analysis, and the Mann-Whitney U test was used for the same outcomes in intragroup analysis. The level of significance was 5% (P < .05). RESULTS: Twenty-seven (27) patients participated (mean age, 51.4 years; mean body mass index, 26.5 kg/m2), with 9 patients in each treatment group. No statistically significant differences were noted in clinical or anthropometric variables among the groups, but mean baseline wound areas were different (2.6 cm² for the control, 1.9 cm² for the LLLT, and 5.5 cm² for the HAM groups). Intragroup comparisons showed a significant reduction in PUSH score in the LLT group between days 0 and 21 (8.2 vs 4.9; P < .01) and days 21 to 28 (4.9 vs 3.2; P < .001). In all treatment groups the percent reduction was significantly different between days 7 and 28. No outcomes were significantly different between groups. CONCLUSION: Diabetic foot ulcer wound area as well as PUSH and VAS scores showed more improvement for patients with DM receiving LLLT or HAM than for the control group, but the differences were not significant. Larger studies are needed to compare these treatment modalities.
Juliana Macêdo Magalhães, Thalia Alves Rodrigues, Marly Marques Rêgo Neta, Carolinne Kilcia Carvalho Sena Damasceno, Kayo Henrique Jardel Feitosa Sousa, and Emília Ângela Lo Schiavo Arisawa
FapUNIFESP (SciELO)
ABSTRACT Objective: To describe, in the mothers’ perception, the experiences lived by families in the care of children with autism spectrum disorder. Method: Qualitative study, carried out with 20 mothers of children diagnosed with autistic disorder accompanied by an institution in Teresina-Piauí, Brazil. Semi-structured interviews were conducted between February and March 2019 and subjected to content analysis. Results: Five central ideas related to the stages experienced by family members after the diagnosis were identified, ranging from denial to acceptance. Family members and caregivers experience feelings of sadness and mourning for the discovery of the impossibility of curing the syndrome, revealing the need for care for this family. The search for help and adaptations of the routine are constant experiences. Conclusion: Caring for children who live with autistic disorder involves learning ranging from structural to emotional aspects, such as dealing with limitations and impossibility of cure, pointing out to the need for family care.
Mayara Moniz Vieira Pinto, Vitor Gabriel Poli de Lima, Leandro José Raniero, and Emilia Angela Lo Schiavo Arisawa
SPIE
Autistic Spectrum Disorder (ASD), considered a neurological development disorder, is characterized by different degrees of deficit in communication, social interaction, and learning, accompanied by repetitive and stereotyped patterns of behavior. The diagnosis of ASD is extremely complex due to the unknown etiopathogenesis and the diversity of symptoms presented by the individuals. This study aimed to evaluate the effectiveness of Fourier Transform Infrared Spectroscopy (FT-IR) as a tool for the diagnosis of ASD in saliva. Saliva samples from 14 volunteers, 7 with ASD (G-A) and 7 Neurotypical (G-N) were analyzed. The collected samples were centrifuged, 20 µL of supernatant was deposited on calcium fluoride (CaF2) windows and dehydrated for 10 min. The FT-IR spectra were obtained by a spectrophotometer coupled to a microscope, in the spectral range of 4000 cm-1 - 900 cm-1 , with 32 scans, and spectral resolution of 4 cm-1 . A total of 112 spectra were collected, and their averages were submitted to Cluster analysis by the second derivative, aiming to group the results by similarity. FT-IR Spectroscopy was able to separate approximately 86% of the samples in the corrected group. The results showed an increase in bands related to proteins and hormones. Individuals diagnosed with ASD often have increased levels of stress, difficulty in interpersonal relationships, and reduced salivary flow compared to neurotypical individuals. ASD patients present a higher concentration of proteins and cortisol release in the saliva. FT-IR spectroscopy is a promising tool to differentiate saliva samples from ASD patients from neurotypical individuals.
Marcele Florêncio das Neves, Diane Cristina Aleixo, Izabela Santos Mendes, Fernanda Púpio Silva Lima, Renata Amadei Nicolau, Emilia Angela Loschiavo Arisawa, Rodrigo Alvaro Brandão Lopes-Martins, and Mário Oliveira Lima
Springer Science and Business Media LLC
Milena Valdinéia da Silva Leal, Mário Oliveira Lima, Renata Amadei Nicolau, Teresa Marly Teles de Carvallho, Jesus Antonio de Carvalho Abreu, Diego Rodrigues Pessoa, and Emilia Angela Lo Schiavo Arisawa
Mary Ann Liebert Inc
Background: Diabetic neuropathy (DN) is one of the major complications developed by individuals with diabetes mellitus. DN is responsible for a high morbidity and mortality rate and impacts the public health and medical assistance resources. Intradermic laser irradiation on blood (ILIB) consists of the application of light beams on the radial arterial, providing anti-inflammatory and vasodilator effects, antiarrhythmic action, reduction of glucose, and stabilization of the hormonal and immunological systems. These effects help to maintain the physiological dynamics of the body. Objective: The goal of this research was to evaluate the effects of ILIB to relieve pain and improve the quality of life in DN patients. The sample comprised 30 diabetic volunteers with DN, randomly distributed into 3 groups: Control-conventional treatment; ILIB-100 mW, 660 ± 10 nm, 30 applications in total, divided into 3 stages of 10 applications, 30 min each, daily, with a 20-day interval between each stage; SILIB-same protocol described for ILIB, with the equipment switched off. Before and after the application of the therapeutic protocols, all volunteers were evaluated by the following instruments: Medical Outcomes Study 36-Item Short-Form Health Survey (SF-36), visual analog scale, Leeds Assessment of Neuropathic Symptoms and Signs (LANSS), and PAIN DETECT scale. Collected data were statistically analyzed with a 95% confidence interval, p < 0.05. Results: The ILIB group presented significantly lower pain levels and a better quality of life compared with the control and SILIB groups. Conclusions: This study demonstrated that ILIB therapy was effective in reducing pain and improving quality of life in patients with DN.
Diego Rodrigues Pessoa, Davidson Ribeiro Costa, Bruno de Moraes Prianti, David Ribeiro Costa, Carolina Alves Delpasso, Emília Ângela Lo Schiavo Arisawa, and Renata Amadei Nicolau
FapUNIFESP (SciELO)
ABSTRACT This case report aims to evaluate the treatment of Temporomandibular Disorder (TMD) of muscular origin by associating facial massage (FM), dry needling (DN), and low-level laser therapy (LLLT). The pre- and post-treatment evaluations consisted of clinical examinations based on the Research Diagnostic Criteria (RDC) for TMD, pain intensity quantification by means of the Visual Analog Scale (VAS), mandibular movement measurement, and electromyographic (EMG) analysis of the masseter and temporal muscles. Post-therapy assessment indicated a decrease in pain sites of 58%, in pain intensity mean (1.3), and an increase in the maximum aperture of 10 mm, in addition to normalization of EMG signals. We conclude that, after application of the treatment protocol, there was a decrease in painful sites, gain in amplitude of mandibular movements, and normalization of EMG activity.
Maria das Graças Bastos Licurci, Alessandra de Almeida Fagundes, and Emilia Angela Lo Schiavo Arisawa
Elsevier BV
Joelita de Alencar Fonseca Santos, Mariana Barbosa Dias Campelo, Rauirys Alencar de Oliveira, Renata Amadei Nicolau, Verônica Elis Araújo Rezende, and Emilia Ângela Loschiavo Arisawa
Mary Ann Liebert Inc
OBJECTIVE
To analyze the efficacy of the therapeutic use of Low-Level Laser Therapy (LLLT) on the tissue repair process of chronic wounds in patients with diabetic feet through the analysis of Pressure Ulcer Scale for Healing (PUSH) scales, pain and the measurement done using the ImageJ© software.
METHODS
This clinical trial was carried out with 18 patients 30-59 years of age, who had chronic wounds on their foot due to complications of diabetes mellitus. The patients were randomly allocated in two different groups of equal numbers: Control and Laser Groups. The LLLT equipment used in the research has a wavelength of 660 nm, 30 mW power, continuous mode emission, 6 J/cm2 dosimetry, 48/48 h in a 4-week period. Measurement and the aspect of wounds were noted in the PUSH scale and the pain was evaluated weekly. The Mann-Whitney U nonparametric test was used to compare groups.
RESULTS
The Laser Group presented a significant increase of the tissue repair index when compared with the Control Group, with a significant statistical difference (p < 0.013). There was no significant difference between the groups in all the weeks using the PUSH scale.
CONCLUSIONS
The use of LLLT on chronic wounds in a diabetic foot demonstrated efficacy on the progression of the tissue repair process in a short period.
Tanmoy T. Bhattacharjee, Mariana C. Nicodemo, Luciana B. Sant'Anna, Emilia A. Lo Schiavo Arisawa, and Leandro Raniero
Wiley
Tendinopathy, an important sports injury afflicting athletes and general public, is associated with huge economic losses. The currently used diagnostic tests are subjective, show moderate sensitivity and specificity; while treatment failures persist despite advances in therapy. This highlights the need for tendinopathy diagnostic and treatment monitoring tools. This study investigates tendon injury, natural healing and effect of treatment using ATR‐FTIR complemented with histopathology. Control (C), injured (I) and treated (T) rat tendons were extracted 3, 7, 14 and 28 days post‐injury/treatment, representing phases of healing; and subjected to hematoxylin & eosin staining as well as spectroscopy. While C showed no change, I‐ and T‐related histological changes could be clearly observed in stained sections. ATR‐FTIR spectra highlighted the biochemical changes within groups. Multivariate analysis could classify C, I and T with 75%; different days between groups with 84%; and different days within group with 65% efficiency. Results suggest that such analysis can not only identify C, I or T but also different phases of healing. Difference between I and T at different time points also suggest change in rate of healing. Further studies may help develop this technique for clinical diagnosis and treatment monitoring in future.
Mariana Barbosa Dias Campelo, Joelita de Alencar Fonseca Santos, Antonio Luiz Martins Maia Filho, Daniel Cabral Leão Ferreira, Luciana Barros Sant’Anna, Rauirys Alencar de Oliveira, Leonardo Fonseca Maia, and Emilia Ângela Loschiavo Arisawa
FapUNIFESP (SciELO)
PURPOSE
To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats.
METHODS
Thirty six rats were submitted to a surgical injury induction and divided into two groups (n = 18): group C (control) and T (treated with the HAM). The macroscopic evolution in the wound area and the histological characteristics of the skin samples were evaluated.
RESULTS
The regression of the wound area was greater in group T. The histological analysis revealed a significant reduction (p < 0.05) in the inflammatory infiltrate in group T at all experimental periods compared with that in the control group. Furthermore, the group T presented a significant increase in the proliferation of fibroblasts at 14 and 21 days compared with group C (p < 0.05). Regarding the deposition of mature collagen fibers, there was an increase in the replacement of type III collagen by type I collagen in group T (p < 0.05).
CONCLUSION
Treatment with the HAM reduced the healing time as well as the inflammatory responses, increased the proliferation of fibroblasts, and induced a higher concentration of mature collagen fibers.
Tanmoy Bhattacharjee, Mariana Nicodemo, Emilia Lo Schiavo Arisawa, and Leandro Raniero
FapUNIFESP (SciELO)
Introduction: Tendinopathy, a disease involving tendon inflammation and damage, results in considerable productivity and economic losses for the patient and the society. Currently available diagnosis/ treatment monitoring modalities are less efficacious and highly subjective, underlying the need for better tools. Previously, we have shown that Fourier Transform Infrared (FTIR) spectroscopy has potential in tendinopathy diagnosis/ monitoring, when combined with multivariate statistical analysis. The current study explores the spectral range that gave the best results in statistical analysis, to discover protein secondary structures associated with tendon injury and recovery that can act as markers of disease. Methods: Animals (n=60) underwent a surgery in which Achilles tendon were injured by dropping a 20g weight. Rats were divided into three groups (n=20) – control (C), Achilles tendon injury (I) and Achilles tendon injury treated with amniotic membrane fragment treated (T). FTIR spectra were obtained from each group 3, 7, 14, and 28 days post injury/ treatment. Results: Triple helix, β-turn, and disordered structure levels differ between control, injured and treated tendons over the time period studied. Parallel β-sheets increase steadily over time in treated tendons compared to control and injured. Conclusion: Combined analysis of Triple helix, βsheets, β-turn, and disordered structure levels may be useful for tendinopathy diagnosis and treatment monitoring. However, further studies in this area are required to confirm the findings.
Aline Carla Teles de Sousa, Ítalo Bruno Paiva da Rocha, Ana Flávia Machado de Carvalho, Nayana Pinheiro Machado de Freitas Coelho, Maura Cristina Porto Feitosa, Esmeralda Maria Lustosa Barros, Emilia Angela Lo Schiavo Arisawa, and Maria Rosilândia Lopes de Amorim
Maad Rayan Publishing Company
Introduction: An ulcer is an injury that affects the skin and has many causes. Healing is a way of protecting the body against any infectious agent that tries to infect you through the wound. Low level laser (LLL) in the treatment of ulcers, aims at maintaining the stability of the physiological process of tissue repair and ultrasound (US) acts by increasing the elasticity of tissue and scarring. The aim of this study was to analyze the effects of LLL and US in wound healing of ulcers induced in mice. Methods: In this experimental study, a sample of 36 guinea pigs was divided into 6 groups of 6 mice, 2 groups as control (C7) and (C14), 2 treated with laser (GL7) and (GL14) and the others 2 treated with ultrasound (GU7) and (GU14). The ulcer was induced and after 7 days, 6 mice from each group were sacrificed to obtain the histological sections for analysis, and the remaining 6 mice from each group continued under treatment and in day 14, they were sacrificed to obtain histologic specimens while macroscopic image was also carried out. Results: Three aspects were analyzed, the percentage of wound regression where it was observed that there was a significant difference in the first 7 days. Regarding the inflammatory process, it was observed that in the first 7 days GL7 and GU7 improved significantly and within 14 days of the experimental period GU14 showed a significant difference when compared to C14. The number of fibroblasts present in the GL at 7 days showed a significant difference compared to the others, and at 14 days, the US group had a significant difference compared to the other groups. Conclusion: Thus, it was evident that the US had more effective results with anti-inflammatory action, better organization and increased deposition of collagen and fibroblasts. Therefore, it can be concluded that other studies are consistent with this taking into account the laser must be used during the early days of the initial healing process and the US during the end of this process.
Mariana de Castro Nicodemo, Lia Renó das Neves, Josafá Carvalho Aguiar, Flaviane de Souza Brito, Isabelle Ferreira, Luciana Barros Sant'Anna, Leandro José Raniero, Rodrigo Álvaro Lopes Martins, Paulo Roxo Barja, and Emilia Angela Lo Schiavo Arisawa
FapUNIFESP (SciELO)
PURPOSE
To evaluate the effect of human amniotic membrane (hAM) fragment on inflammatory response, proliferation of fibroblast and organization of collagen fibers in injured tendon.
METHODS
Sixty rats were divided into 3 groups: C - surgical procedures without tendon lesion and with simulation of hAM application; I - surgical procedures, tendon injury and simulation of hAM application; T - surgical procedures, tendon injury and hAM application. These groups were subdivided into four experimental times (3, 7, 14 and 28 days). The samples underwent histological analysis and ATR-FTIR spectroscopy.
RESULTS
Histological analysis at 14 days, the T group showed collagen fibers with better alignment. At 28 days, the I group presented the characteristics described for the T group at 14 days, while this group presented aspects of a mature connective tissue. FT-IR analysis showed a clear distinction among the three groups at all experimental times and groups T and I presented more similarities to each other than to group C.
CONCLUSION
Acute injury of tendon treated with human amniotic membrane fragment showed a faster healing process, reduction in inflammatory response, intense proliferation of fibroblasts and organization of collagen fibers.
Maura Cristina Porto Feitosa, Ana Flávia Machado de Carvalho, Valrian Campos Feitosa, Rauirys Alencar de Oliveira, Nayana Pinheiro Machado de Freitas Coelho, Veruska Cronemberger Nogueira Rebêlo, and Emília Ângela Loschiavo Arisawa
Centro Universitario Sao Camilo - Sao Paulo
Maria das Graças Bastos Licurci, Alessandra De Almeida Fagundes, and Emilia Angela Lo Schiavo Arisawa
EDIPUCRS
***Whole body vibration and blood glucose levels in elderly people: a pilot study***AIMS: To evaluate the effect of whole-body vibration therapy on blood glucose levels of elderly people using a vibratory platform.METHODS: The study included volunteers of both genders, aged between 60 and 75 years, without gait disorders or motor disabilities. Individuals with systemic disease, obesity, conflicting treatments, tobacco users, and those who could not understand the maneuvers were excluded. The study consisted of a single session of whole-body vibration. Heart rate, respiratory rate, arterial blood pressure, and blood glucose were obtained for each participant at baseline, immediately before the procedure. Volunteers were then placed in the standing position on the ground of an oscillating platform and treated for 10 min with a frequency of vibration set at 20 Hz (displacement ±6 mm; orbital vibration). Immediately after the whole-body vibration session, all data were collected again. Comparisons were made between baseline and post-treatment data. Normality was tested by Kolmogorov-Smirnov test. Data were analyzed using paired Student’s t-test or Wilcoxon test as appropriate. The level of significance was set at p<0.05.RESULTS: Eleven participants (seven men, four women) participated of the study. The mean age was 64.18±4.37 years. The results showed a significant decrease in the glycemic indexes (p=0.016) and an increase in the respiratory rate (p=0.047) after the whole-body vibration session.CONCLUSIONS: Healthy elderly subjected for 10 minutes to whole-body vibration set at 20 Hz presented reduction of blood glucose levels and increasing of respiratory rate.
Davidson Ribeiro Costa, David Ribeiro Costa, Diego Rodrigues Pessoa, Leandro Júnio Masulo, Emília Ângela Lo Schiavo Arisawa, and Renata Amadei Nicolau
EDIPUCRS
*** Effect of LED therapy on temporomandibular disorder: a case study ***AIMS: To evaluate the effect of light emitting diode (LED) therapy on temporomandibular disorder.CASE DESCRIPTION: A woman diagnosed with temporomandibular disorder was subjected to four LED therapy sessions at a seven-day interval. In the initial examination of the temporomandibular joint the patient presented a mouth opening without pain of 23 mm, a maximum opening of 25 mm and a maximum opening with the aid of 27 mm. After the treatment, there was an increase of 7 mm in the opening without pain between the first and last evaluation, while the maximum opening of the mouth and the maximum opening with aid increased 6 mm between the first and last evaluation. After 21 days of treatment, a 50% reduction in painful sites was detected on the palpation examination. After the end of the treatment, the visual analog pain scale detected a decrease in pain intensity on both sides, and there was a decrease in the total mean pain intensity. By analyzing the responses to the Medical Outcomes Study 36-item Short Form Health Survey (SF-36), of the eight quality of life domains evaluated, four showed improvement. CONCLUSIONS: In this case study, after LED therapy there was reduction in pain intensity and increase of the mandibular range of motion. The resolution of the signs and symptoms of temporomandibular disorder resulted in patient’s better quality of life.
Marcele Florêncio das Neves, Mariana César Ribeiro dos Reis, Eliana Aparecida Fonseca de Andrade, Fernanda Pupio Silva Lima, Renata Amadei Nicolau, Emília Ângela Loschiavo Arisawa, Adriano Oliveira Andrade, and Mário Oliveira Lima
Springer Science and Business Media LLC
Veruska Cronemberger Nogueira, Leandro Raniero, Guilherme Bueno Costa, Nayana Pinheiro Machado de Freitas Coelho, Fernando Cronemberger Miranda, and Emília Ângela Loschiavo Arisawa
Mary Ann Liebert Inc
Objective: Compare the efficacy of light-emitting diode (LED) and therapeutic ultrasound (TUS), combined with a semipermeable dressing (D), at forming collagen in skin lesions by morphometry and Fourier transform infrared spectroscopy (FT-IR). Materials and Methods: Surgical skin wounds (2.5 cm) were created on 84 male Wistar rats divided into four groups (n=21): Group I (Control), Group II (LED), Group III (LED+D), and Group IV (US+D). On days 7, 14, and 21, the tissue samples were removed and divided into two pieces, one was used for histological examination (collagen) and the other for FT-IR. Results: The histomorphometric analysis showed no significant differences among groups for collagen deposition at 7 days. However, at 14 days, more deposition of collagen was noted in the groups LED (p<0.05) and LED+D (p<0.001) than in the control. At 21 days, the groups LED, LED+D, and US+D presented significantly greater deposition of collagen when compared with the control. The FT-IR spectra, at 14 days, LED+D ...
Ana Flávia Machado de Carvalho, Maura Cristina Porto Feitosa, Nayana Pinheiro Machado de Freitas Coelho, Veruska Cronemberger Nogueira Rebêlo, Juçara Gonçalves de Castro, Patrícia Regina Gomes de Sousa, Valrian Campos Feitosa, and Emilia Angela Lo Schiavo Arisawa
FapUNIFESP (SciELO)
Abstract OBJECTIVE To evaluate the effects of low-level laser therapy isolated and associated with Calendula officinalis oil in treating diabetic foot ulcers. METHOD An experimental, randomized, controlled, prospective, interventional clinical case study using a quantitative approach. The sample consisted of 32 diabetic patients of both genders. Participants were randomly divided into four groups. Doppler Ultrasound evaluation of the Ankle-Brachial Index, brief pain inventory and analog pain scale were performed at baseline and after 30 days. RESULTS Reduced pain was observed in the Low-level laser therapy and Low-level laser therapy associated with Essential Fatty Acids groups (p<0.01). Regarding the Ankle-Brachial Index and Doppler Ultrasound, all groups remained stable. By analyzing lesion area reduction, Low-level laser therapy associated with Essential fatty acids group showed a significance of p=0.0032, and the Low-level laser therapy group showed p=0.0428. CONCLUSION Low-level laser therapy, performed alone or associated with the Calendula officinalis oil was effective in relieving pain and accelerating the tissue repair process of diabetic foot.
Luciana B. Sant'anna, Raduan Hage, Maria Angélica G. Cardoso, Emilia A. L. Arisawa, Maria Martin Cruz, Ornella Parolini, Anna Cargnoni, and Nilson Sant'anna
SAGE Publications
Liver fibrosis is characterized by excessive accumulation of extracellular matrix components in the liver parenchyma that distorts the normal architecture and hepatic function. Progressive fibrosis could end in the advanced stage known as cirrhosis, resulting in the need to resort to liver transplantation. Amniotic membrane (AM) has emerged as an innovative therapeutic approach for chronic liver diseases due to its anti-inflammatory, antiscarring, and wound-healing effects. We have recently shown that AM can be used as a patch on the liver surface at the same time of fibrosis induction, resulting in significantly reduced progression and severity of biliary fibrosis. Here we investigated the effects of human AM on the established rat model of liver fibrosis, induced by the bile duct ligation (BDL). We also explored the effect of AM on the expression of transforming growth factor-β1 (TGF-β1), the main profibrogenic factor in hepatic fibrosis, and the proinflammatory cytokines, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and anti-inflammatory cytokine IL-10. Two weeks after BDL, the liver was covered with a fragment of AM or left untreated. Six weeks later, the fibrosis was first assessed by the semiquantitative Knodell and the METAVIR scoring systems and, thereafter, by CellProfiler digital image analysis to quantify the area occupied by collagen deposition, ductular reactions (DRs), activated myofibroblasts, and TGF-β1. The hepatic cytokines were determined by ELISA. AM-treated rats showed a significantly lower score compared to the control BDL rats (2.5 ± 0.9 vs. 3.5 ± 0.3, respectively; p < 0.05). The collagen deposition, DRs, number of activated myofibroblasts, and TGF-β1 were all reduced to about 50% of levels observed in untreated BDL rats. These findings suggest that AM, when applied as a patch onto the liver surface, is useful for treating well-established cholestatic fibrosis, and the mechanism was partly by means of downregulating the profibrotic factor TGF-β1 and IL-6.
Maura Cristina Porto Feitosa, Ana Flávia Machado de Carvalho, Valrian Campos Feitosa, Isabely Madalena Coelho, Rauirys Alencar de Oliveira, and Emília Ângela Loschiavo Arisawa
FapUNIFESP (SciELO)
PURPOSE
To evaluate the effects of the low-level laser therapy applying Laser on the tissue repair in ulcer carriers due to diabetes.
METHODS
Sixteen type II diabetic patients, ulcer carriers in the lower limbs, participated in the research from which eight were in the control group and eight were submitted to the low-level laser therapy with a pulsed wave form, visible ray, wave length of 632.8 nm, 30 mW peak power, (Laser - HTM). The application time was of 80 (4J/cm2) seconds. The application was punctual without contact (approximately 1mm of distance), the pen being held in a perpendicular position related to the wound, in equidistant points. There were 12 appointments, of which three were done weekly in alternated days. Photograph records and an application of the brief inventory of pain were done before and after 30 days of follow-up.
RESULTS
There was a significant decrease in the size of the wound when compared to the control group (p<0.05). The pain was also reported as having an intense improvement in the treated group.
CONCLUSION
The low-level laser treatment seems to be an efficient method, viable, painless and of low costs concerning the tissue repair ulcers in a diabetic foot.
Ana Flávia Machado de Carvalho, Paula Fernanda Batista de Sousa, Maura Cristina Porto Feitosa, Nayana Pinheiro Machado de Freitas Coelho, Esmeralda Maria Lustosa Barros, Valrian Campos Feitosa, and Emília Angela Loschiavo Arisawa
FapUNIFESP (SciELO)
PURPOSE
To analyze the effects of the low-level laser therapy in the acute myositis induced in rats.
METHODS
Twelve rats were subjected to bilateral ovariectomy for inducing osteoporosis. After surgery, they were divided into two groups: Ovariectomy-control group (G1, n=6), receiving 0.5 ml distilled water by gavage for 30 days, and Ovariectomy plus mastruz group (G2, n=6), receiving 0.5 ml of the hydroalcoholic extract of mastruz at 10% concentration (50mg) daily, for the same period. Then, the blood of the animals was collected for further biochemical analysis (liver function) and tibia and liver were removed for histological and histomorphometric analyses.
RESULTS
In the MT group there was a statistic significant decrease in the number of inflammatory cells, related to the MI group (p<0.05), increase in the fibroblastic proliferation, when compared to groups C and MI related to MT group (p<0.01) and statistic significant edema regression (p=0.0400) in the MT group
CONCLUSION
The low-level laser therapy was efficient in the reduction of the inflammatory process, increase of the fibroblastic proliferation and the reduction of the edema.