Zainal Abidin Muchlisin
@unsyiah.ac.id
Faculty of Marine and Fisheries
Universitas Syiah Kuala
EDUCATION
Ph.D in Applied Ichthyology from Universiti Sains Malaysia
RESEARCH, TEACHING, or OTHER INTERESTS
Aquatic Science, Animal Science and Zoology
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Siti Maulida, Kartini Eriani, Nur Fadli, Mohd N. Siti-Azizah, Filiz K. Kocabas, Mehmet Kocabas, Martin Wilkes, Luvi S. Handayani, Sri R. Rahayu, and Zainal A. Muchlisin
Elsevier BV
Nur Fadli, Sri Jumiati, Nanda Muhammad Razi, Adrian Damora, Zainal A. Muchlisin, Irma Dewiyanti, Mutia Ramadhaniaty, Essy Harnelly, Ahasan Habib, and Mohd Nor Siti-Azizah
Bogor Agricultural University
Groupers are among the fish groups that are difficult to recognize due to their high morphological similarities. Therefore, molecular techniques, particularly DNA barcoding, are extensively utilized to differentiate this fish group. This study aimed to analyze and validate six grouper species belonging to the Epinephelidae family that were harvested from Langsa district waters in Aceh province, Indonesia, based on DNA barcode data. It was conducted from June to December 2021, with the fish specimens collected from fishers at fish landing sites and the fish market in Langsa City, Aceh province. A total of 22 grouper sequences belonging to six species were generated, namely Epinephelus coioides, E. bleekeri, E. malabaricus, E. erythrurus, E. sexfasciatus and Mycteroperca poecilonotus (formerly Epinephelus poecilonotus). Genetic distance within these species ranged from 0.10 to 0.73% (average: 0.40%). Notably, E. malabaricus and E. coioides exhibited the closest genetic kinship (4.07%), while E. sexfasciatus and M. poecilonotus displayed the greatest genetic distance (19.33%). This study provides the first DNA reference for grouper in Langsa district, Indonesia, with significant implications for future sustainable grouper management.
Muh. Nur Hidayat, Reza Wafdan, Marwan Ramli, Zainal A. Muchlisin, Ichsan Rusydi, Muhammad Muhammad, and Syamsul Rizal
EDP Sciences
This study investigates the relationship among chlorophyll-a (Chl-a), sea surface temperature (SST), and sea surface salinity (SSS) in Aceh Waters, Indonesia using data from January and July 2022. Chl-a, SST, and SSS data were retrieved from the Copernicus Marine Environment Monitoring Service (CMEMS) database. Pearson correlation analysis was employed to assess the connections among Chl-a, SST, and SSS within the Aceh Waters region. The findings reveal that all three parameters - Chl-a, SST, and SSS - conform to the seasonal monsoon patterns observed in January and July 2022. The correlation analysis conducted for January revealed the following relationships: a negative correlation between Chl-a and SST (-0.649), an inverse correlation between Chl-a and SSS (-0.215), and a positive correlation between SST and SSS (0.493). Conversely, correlations for July reveal a negative correlation between Chl-a and SST (-0.503), a positive correlation between Chl-a and SSS (0.039), and a negative correlation between SST and SSS (-0.478). Overall, this study elucidates the complex relationship among Chl-a, SST, and SSS in Aceh Waters, which is influenced by seasonal monsoon variations. Understanding this relationship is essential for assessing marine environmental dynamics and their potential impacts on ecosystems and human activities in the region.
Nanda Muhammad Razi, Muhammad Nasir, Mutia Ramadhaniaty, Sri Riska Rahayu, Luvi Syafrida Handayani, Ahmad Sazali A. Sitrous, Cut Tria Aswina, Nur Fadli, and Zainal A. Muchlisin
EDP Sciences
Anabas testudineus, the climbing perch, is a valuable and well-liked freshwater fish with significant commercial worth. This fish is found in Indonesia and other parts of Asia. Nonetheless, there hasn't been much research done on the genetic relationships across Asian populations of climbing perch. Thus, the aim of this work was to use the mitochondrial COI gene to examine the phylogeography of A. testudineus in freshwater Asia. The population sample was taken from South Aceh, Indonesia. The sample was extracted using a modified C-TAB protocol. In all, 48 sequences from the Genbank and three sequences from South Aceh were included in this investigation. Seven samples came from Indonesia, three from Malaysia, nine from Philippines, seven from Vietnam, eight from Thailand, ten from India, and four from Bangladesh. According to the findings, the sequences formed 17 different haplotypes. For every population, the haplotype diversity (Hd) value is 0.897. The genetic distance measuring the closest populations is 0.0007 between Indonesia and Philippines, whereas Indonesia and Vietnam populations are the furthest apart, at 0.0904. Between 0 and 0.0157 is the genetic distance within the population. Thus, the populations of A. testudineus in Aceh and Indonesia and the Philippines are genetically more similar.
Muh. Nur Hidayat, Reza Wafdan, Marwan Ramli, Zainal A. Muchlisin, Sugianto Sugianto, Makwiyah A. Chaliluddin, Thaib Rizwan, and Syamsul Rizal
EDP Sciences
This study analyzes the sea currents, temperature, and salinity in the Malacca Strait during the northeast (NE) and southwest (SW) monsoons of 2022, represented by monthly average data from the Copernicus Marine Service (CMEMS) data portal for January and July, respectively. Vertical sections are created to visualize the layer structure and variations of sea parameters within the water column. The findings reveal differences in sea currents, temperature, and salinity between the NE and SW monsoons. July (SW monsoon) records consistently warmer temperatures than January (NE monsoon), both at the sea surface and seabed. Surface salinity is higher in July than in January, while seabed salinity is greater in January than in July. In January, the salinity-influenced mixed layer depth (MLD) appears to be thicker than the temperature-influenced MLD. There are no significant differences in the thickness of the temperature- and salinity-influenced MLDs during July. These variations in sea currents, temperature, and salinity at different depths provide a comprehensive understanding of the marine environmental structure and dynamics of the Malacca Strait.
Siti Maulida, Kartini Eriani, Nur Fadli, Luvi S. Handayani, and Zainal A. Muchlisin
EDP Sciences
Sperm cryopreservation is a sperm storage method that needs to be developed to ensure the availability of climbing perch fish (Anabas testudineus) sperm considering the difficulty of providing quality broodstock. This study aims to determine the effect of several types and concentrations of cryoprotectants on the DNA integrity of climbing perch fish sperm after cryopreservation for 14 days at a temperature of -196 ℃. Analysis of sperm DNA integrity used the gel electrophoresis method. This research consists of four types of cryoprotectants; Each type of cryoprotectant consists of two concentration levels. The cryoprotectant concentrations used were DMSO (concentrations 10 and 20%), Methanol (concentrations 10 and 20%), Glycerol (concentrations 5 and 20%), and Ethanol (concentrations 5 and 15%) as well as control treatment and fresh sperm treatment. Based on the results of the DNA integrity analysis of climbing perch fish sperm, it showed that all sperm DNA samples did not form fragments, meaning that there was no damage to climbing perch fish sperm DNA in all samples tested, both fresh sperm samples and post-cryopreserved sperm samples.
Sri Riska Rahayu, Zainal A. Muchlisin, Nur Fadli, Syamsul Rizal, Ilham Raza, Muhammad Isran Lazuardi, Nanda Muhammad Razi, Luvi Syafrida Handayani, and Firman M. Nur
EDP Sciences
Lutjanus or snappers usually known as economical fish in Indonesia and worldwide. This research aims to observe the different phylogeography correlation of Lutjanus bengalensis in Indonesia Waters and South China Sea used COI gene marker. The primary data collected from fish port Pulo Aceh, Indonesia (5 sequences), while the population of others region such as Bali (3 sequences) and Maluku (3 sequences) was retrieved from NCBI GenBank. South China Sea represented by sampled from Taiwan (3 sequences) and Hongkong (3 sequences) that also retrieved from NCBI Genbank. In total 17 sequences were analysed the diversity of haplotypes 0-1 and the diversity of nucleotide 0-0.030. Based on this researched data, it will become basic data for spesies management for Lutjanus bengalensis in Indonesia.
Sri Riska Rahayu, Syamsul Rizal, Nur Fadli, Ilham Raza, Muhammad Isran Lazuardi, Luvi Syafrida Handayani, Nanda Muhammad Razi, Martin Wilkes, Mohd Nor Siti-Azizah, and Zainal A. Muchlisin
Springer Science and Business Media LLC
Mutia Ramadhaniaty, Maria Ulfah, Nurfadillah Nurfadillah, Tien Amienatun, Muhammad Syukran, Nanda M. Razi, Khalidin Khalidin, and Zainal A. Muchlisin
Walter de Gruyter GmbH
Summary Macrozoobenthos is a sedentary aquatic biota and is often used as a bioindicator for assessing water conditions. Therefore, this study aimed to investigate the species diversity of the macrozoobenthos and to analyze the heavy metal contamination in Faunus ater in the Rasian and Asahan estuaries in Aceh Province, Indonesia. The sampling was carried out in July 2022 on a squared transect of 1 m × 1 m for the collection of macrozoobenthos samples at random locations. The results showed that there were 17 macrozoobenthos species from both locations, of which 14 species were found in the Rasian River estuary and seven species in the Asahan River estuary. The diversity index of macrozoobenthos was in a moderate category. Terebralia sulcata had a higher density in the Asahan River, while F. ater had a higher density in the Rasian River. The results of the heavy metal analysis in the F. ater sample showed that this macrozoobenthos was contaminated by mercury, cadmium, and lead, of which the concentration of mercury exceeded the threshold limit, and they are, therefore, not safe for consumption.
Tin-Yam Chan, Zainal Abidin Muchlisin, and Amirah Hurzaid
Oxford University Press (OUP)
Abstract The name Penaeus simplex, given to a new species of prawn species by Tin-Yam Chan, Zainal Abidin Muchlisin, and Amirah Hurzaid (2021) in an article published in Journal of Crustacean Biology (https://doi.org/10.1093/jcbiol/ruaa096) is deemed to be unavailable according to the International Code of Zoological Nomenclature (Code) because that article is an electronic-only publication and did not include a ZooBank registration number (LSID). The present note serves to validate the name Penaeus simplex by fulfilling the requirements of the Code.
Nur Fadli, Adrian Damora, Zainal Abidin Muchlisin, Mutia Ramadhaniaty, Nanda Muhammad Razi, Sri Riska Rahayu, Edison D. Macusi, Ahasan Habib, and Mohd N. Siti-Azizah
Elsevier BV
Muhammad Radhi, Firdus Firdus, Sri Riska Rahayu, Mikhail Solovyev, and Zainal A. Muchlisin
Elsevier BV
Nur Fadli, Adrian Damora, Zainal A. Muchlisin, Irma Dewiyanti, Mutia Ramadhaniaty, Nanda Muhammad Razi, Jamsari Amirul Firdaus Jamaluddin, Edison D. Macusi, and Mohd Nor Siti-Azizah
Bogor Agricultural University
Understanding the patterns of genetic diversity of species and precise stock identification are important in fisheries conservation management. However, studies on genetic diversity, connectivity, population structure, and gene flow of groupers within the Indo-Malaya Archipelago (IMA) waters are limited. The objective of the present study was to examine the phylogeographic patterns of the family Epinephelidae in IMA waters based on a parallel study of five selected species utilizing the mitochondrial COI. The grouper species were: areolate grouper (Epinephelus areolatus), blacktip grouper (Epinephelus fasciatus), six-bar grouper (Epinephelus sexfasciatus), blue-lined hind (Cephalopholis formosa), and white-edged lyretail (Variola albimarginata). Specimens were obtained from fish landing sites and fish markets from 23 locations throughout the IMA waters. This study showed genetic structuring for two species (Epinephelus areolatus and Variola albimarginata) but genetic homogeneity for the other three investigated species across IMA. Various geological and demographic histories, local and regional oceanographic features, and biological characteristics are hypothesized to shape the present genetic pattern of each species across the IMA waters. The establishment of effective international cooperation is encouraged to manage grouper species stocks in this region.
Irma Dewiyanti, Darmawi Darmawi, Zainal A. Muchlisin, Teuku Z. Helmi, and Iko I. Arisa
International Information and Engineering Technology Association
Nurlaili Nurlaili, Kartini Eriani, Itsnatani Salma, Siti Maulida, Sri Riska Rahayu, Luvi Syafrida Handayani, Filiz Kutluyer Kocabas, Mohd Nor Siti-Azizah, Martin Wilkes, and Zainal Abidin Muchlisin
CryoLetters Limited Liability Partnership
BACKGRUND: Goldfish Carassius auratus is a popular ornamental fish extensively cultured worldwide. Sperm cryopreservation is a common fish breeding method that ensures sperm availability around the year. Studies on cryopreservation of goldfish sperm, especially on the suitability of cryoprotectant types and pre-freezing time, are scarcely available. OBJECTIVE: To determine the most suitable type of cryoprotectant and pre-freezing for the successful cryopreservation of goldfish sperm. MATERIALS AND METHODS: A completely randomized design with two factors was utilized in this study. The first factor is the type of cryoprotectants, which included methanol, ethanol, ethylene glycol, glycerol, and DMSO. The second is pre-freezing times of 10, 20, 30, and 40 min at each of the pre- freezing temperatures of 4°C, −10°C, and −79°C, meaning that the total times for the ramping down of temperature were 30, 60, 90 and 120 min, respectively. The Ringer solution and 10% egg yolk were used as extender and extracellular cryoprotectant. The sperm was stored at −179°C for 7 days. RESULTS: The ANOVA test showed that cryoprotectants and pre-freezing significantly affected the motility, viability, and fertility of goldfish sperm after freezing in liquid nitrogen for 7 days (ρ<0.05). Furthermore, 10% DMSO combined with 15% egg yolk with an pre-freezing time of 20 min can maintain sperm motility, viability, and fertility higher than other treatments, by 79%, 80%, and 33%, respectively. The agarose gel electrophoresis showed no DNA fragmentation in all samples, including fresh sperm. CONCLUSION: We conclude that 10% DMSO combined with 15% egg yolk and 20 min pre-freezing is the best treatment for goldfish sperm cryopreservation.
Siti Maulida, Kartini Eriani, Nur Fadli, Filiz Kutluyer Kocabaş, Mohd Nor Siti-Azizah, Martin Wilkes, and Zainal Abidin Muchlisin
Elsevier BV
Sri Riska Rahayu, Zainal A. Muchlisin, Nur Fadli, Nanda Muhammad Razi, Mutia Ramadhaniaty, Luvi Syafrida Handayani, Siti Maulida, Firman M. Nur, Nurlaili Nurlaili, and Moh N. Siti-Azizah
Elsevier BV
U Nisa, W Aminarah, Z A Muchlisin, and A W Perdana
IOP Publishing
Abstract Fighting fish Betta dennisyongi is a popular ornamental freshwater fish in Indonesia. Male fighting fish are preferred because the colour pattern and shape of the fins are more attractive so they are more expensive. This study aims to determine the optimum temperature of rearing media water on the male sex ratio and survival rate of fighting fish larvae. A completely randomized design method with three replications was used in this study. The tested rearing temperature water was: 26°C, 28°C, 30°C, and 32°C. Fish were kept for 40 days. The results of the ANOVA test showed that larval rearing temperature affected significantly the ratio of male and female fish larvae survival (P<0.05). The highest percentage of the male sex was found at a temperature of 32 °C (93.33%), this value was significantly different from other temperature treatments. Meanwhile, the highest larval survival was obtained at a rearing temperature of 28 °C (80.00%), where this value was significantly different from other treatments. Therefore, with economic considerations, it is concluded that the optimal larval rearing media temperature is 28 °C.
S R Rahayu, Z A Muchlisin, N Fadli, S Rizal, N M Razi, M Ramadhaniaty, L S Handayani, S Maulida, F M Nur, and N Nurlaili
IOP Publishing
Abstract Lutjanus kasmira is one of the snappers occupying and commonly found in the Arabian Sea and the Bengal Strait. The purpose of this study was to examine the comparative phylogeography pattern of Lutjanus kasmira in the Bengal Strait and the Arabian Sea using the mitochondrial COI gene as a marker. The population sampled of Indonesia was taken from fish landing in Banda Aceh, Indonesia, while the population of India represented Bengal Strait, Oman, Yemen, Djibouti and Somalia respresented Arabian sea was retrieved from the NCBI Genbank. The extraction method was carried out using a modified C-TAB protocol. In this study at total of 47 sequences were analysed that produced 22 haplotypes where 18 haplotypes distributed in the Arabian Sea and 4 haplotypes from Bengal Strait. 10 samples of Oman population produced 6 haplotypes, 10 samples from Djibouti produced 5 haplotypes, 5 haplotypes from the population of Yemen, Somalia 2 haplotypes and India 1 haplotypes, respectively. Meanwhile, the Indonesian population, from 6 samples, produced 3 haplotypes. L. kasmira population from Arabian Sea and Bengal strait had no connectivity. The highest haplotype diversity (Hd) value was found in Oman population of 0.956 and the lowest found in Somalia population, while Indonesia population has a moderate genetic diversity.
L S Handayani, Z A Muchlisin, K Eriani, S Maulida, S R Rahayu, and F M Nur
IOP Publishing
Abstract Walking catfish Clarias batrachus is a freshwaterr fish that has high economic value. Currently, the culture of this local catfish has been initiated in Indonesia, but the breeding technology has not been well developed. Therefore, this study aims to explore the type of natural extender for sperm preservation of local catfish C. batrachus so that it can support the development of its breeding technology, especially in remote areas. The research was conducted at the Hatchery Laboratory of the Faculty of Marine and Fisheries, Universitas Syiahh Kuala, Banda Aceh, Indonesia.. Four types of natural extenders were tested in this study, namely young coconut water, nira water, sugarcane water, and old coconut water. Completely randomized design method consisting of five treatments and three replications were used in this study. Sperm were diluted with each tested extender with a sperm dilution rate of 1:20 (sperm: extender, v/v), then stored at 4 °C. The motility and viability were monitored every 24 h interval for 6 days. The results showed that the natural extender significantly affected the motility, viability, and sperm fertility of walking catfish (P<0.05). Young coconut water produced higher motility and viability on all observation days. The higher sperm motility, viability, and egg fertility on the last day of the experiment were also found in sperm diluted in young coconut water, with values of 45.83%, 43.83%, and 41.50%, respectively. These values were significantly different from other types of extenders. Therefore, it is concluded that young coconut water is the most suitable natural extender for sperm preservation of local catfish at 4 °C.
Su Khi Ng, Cher Chien Lau, Min Pau Tan, Siti Azizah Mohd Nor, Muhd Danish-Daniel, Nor Afiqah-Aleng, Zainal Abidin Muchlisin, and Nur Fadli
Informa UK Limited