Muhammad Fahyu Sanjaya, S.P., M.P

Scopus Publications

Bacterial communities of Iradiator Gamma Karet Alam (IRKA) pool water
I Sugoro, A Mujiyanto, D Tetriana, T Tjiptosumirat, B J Tuasikal, N Robifahmi, F J Rahma, R Santiara, M M Fadhlany, M Y Yunus,et al.
IOP Publishing
Abstract Bacteria which are living in extreme environment have potential for bioprospection as candidates for drug discovery and bioremediation. Radiation-resistant bacteria can be obtained from environments that have radiation sources. Pool water containing radioactive material is one of the important components in the Iradiator Gamma Karet Alam (IRKA) facility. Research on the presence of bacterial communities in the IRKA cooling pool water has never been conducted. This study was conducted to determine the bacterial community of IRKA cooling pool water using Nanopore Sequencing. The study identified 7 phyla, with Proteobacteria and Firmicutes as the dominant with 95.9 and 3.9%, respectively. Phreatobacter, specifically P. oligotrophus, emerged as the dominant genus and the most prevalent species, constituting 71.22% of the cooling pool water samples at the IRKA facility. The limited number of studies conducted on these dominant species presents an opportunity for further exploration of their potential benefits.

Sulphate-reducing bacteria (SRB) in interim storage of spent nuclear fuel
D S Rahayu, L Ambarsari, S E Shalsabilla, A Fadilah, M F Sanjaya, R R Sambari, F Ramadhan, M S Rijal, I G Nadyaputri, and I Sugoro
IOP Publishing
Abstract Interim Storage of Spent Nuclear Fuel (ISSF) is an installation for temporarily storing spent nuclear fuels. Nuclear fuel storage pools must be free from contamination by corrosion-causing microorganisms such as Sulphate Reduction Bacteria (SRB). This research aims to detect SRB on the pool’s wall, floor, and spent nuclear fuel racks. The measured parameters consisted of physicochemical, total bacteria, total SRB, and detection of SRB on the wall, floor, and spent fuel rack in the pool using the SRB kit. The results showed that the quality of the water chemistry in the pool was within limits according to the provisions of the International Atomic Energy Agency, except for the total bacteria (> 1000 CFU/ml). In the water pools around the racks of spent fuels, SRB was also detected at 30 CFU/ml, while the level of SRB on the walls, floors, and spent nuclear fuel racks showed varied values from moderate to severe. This research is important especially to identify corrosion-causing microbes for water and biofilm samples. This is the first step in understanding the problem of biofilm formation and contamination of microorganisms in spent fuel storage pools.

The growth character of sago palm based on differences in time of trunk formation
D R Sari, M F Sanjaya, I Arham, and K Osozawa
IOP Publishing
Abstract This study aimed to (1) characterized sago palms with different trunk formation times; (2) studied the growth characteristics related to the diameter of the sago trunk. This research was conducted at the Sago Cultivation Experimental Site located in Pengkajoang Village, West Malangke District, North LuwuRegency.Thegrowth characters studied were number of leaves, trunk diameter, leaf length,petiole length, tree height,rachis length, number of suckers, length of the most extended leaflets, number of leaflets and the width of broadest leaflets. A paired T-Test was used to analyze the average of three variables between sago-forming trunks at 3 years, 3.5 years, and 4 years after transplanting. In addition to correlation analysis, variables were compared based on their relationships.The sago palm that formed the trunk at 3 years after transplanting had the highest number of leaves (20 strands), 3.5 years (17 strands), and 4 years (16 strands). Conversely, sago palms that form trunks 4 years after transplanting have the widest leaflets(7.44 cm), 3.5 years (6.04 cm) and 3 years (6.52 cm). Based on correlation analysis and path analysis, it is known that the number of leaves, tree height and rachis length are characteristics of sago palm growth which have a significant effect on the formation of trunk diameter.

Seaweed extract effect on arbuscular mycorrhizae spore in soil engineered by earthworm, and the soil effect on upland rice growth
L M H Kilowasid, R Ariansyah, L Afa, G A K Sutariati, Namriah, T C Rakian, M F Sanjaya, and W S A Hisein
IOP Publishing
Abstract Seaweed extract is known to contain nutrients and growth-regulating substances that affect soil biota, and a source of protection against pests and diseases. Earthworm, which is an example of a soil biota and playing the role of ecosystem engineer, has the ability to produce suitable land biostructures, for the inhabitation of arbuscular mycorrhizal fungi (AMF), which has an impact on upland rice growth. Therefore, this study aims to determine, (i) the effect of seaweed extract on the population of earthworms and spores of arbuscular mycorrhizal fungi, and (ii) the impact of the engineered soil on the growth of local upland rice varieties. Furthermore, the extract of seaweed, such as Kappapychus alvarezii, was divided into five concentration levels, namely 0%, 20%, 40%, 60%, and 80%. Each treatment was drenched into the soil from the cogongrass vegetated area, mixed with 20 Pheretima sp., and maintained for 49 days in the greenhouse. The result showed that the total difference in the earthworms’ concentration treatments was not significant. It also showed that the total AMF spores in the engineered soil products of 20% concentration was the highest. Based on treatment with the earthworm engineered soil products, the highest and lowest vegetative growth and yield components of upland rice were observed at the concentrations of 80% and 0%, respectively. In conclusion, the application of seaweed extract to the soil did not significantly reduce the earthworm population. The extract concentration of 20% also increased the total AMF spore in the engineered soil. Moreover, highly treated engineered soil products increased the growth and yield components of upland Kambowa rice on cogongrass soils.

The use of soil biostructures created by soil fauna ecosystem engineers fed with different organic materials as inoculum source of arbuscular mycorrhiza fungi on cocoa seedling
Laode Muhammad Harjoni Kilowasid, Muhammad Fahyu Sanjaya, Laode Sabaruddin, Rachmawati Hasid, Darwis Sulaeman, and Andi Nurmas
Universitas Sebelas Maret
Soil fauna as ecosystem engineers have the ability to create soil biostructures, with the capacity to save arbuscular mycorrhizal fungi (AMF) spores. This study therefore aims to investigate the AMF spore density in the biostructures created by cooperation between earthworms and ants with a different organic matter composition, and to analyze the biostructures’ potential as a source of AMF inoculum on cocoa seedlings. In the first experiment, a combination of earthworms and ants composition, as well as a mixture of G. sepium leaf (GLP), cocoa shell bean (CSB), and sago dregs (SD), was tested. Meanwhile, in the second experiment, the effect of biostructures on cocoa seedlings grown in unsterile soil,was examined. According to the results, the highest AMF spore density was obtained using 20 earthworms+10 ants with 50%GLP+50%CSB + 0%SD treatment. Furthermore, the total AMF spores were positively correlated with the total P value, but negatively correlated with the C/N ratio. Therefore, biostructure application increased AMF spores number in rhizosphere and the cocoa seedling’s root infection. Furthermore, biostructures resulting from the collaborative activity between different soil fauna ecosystem engineers were able to transmit AMF spores to infected plant roots growing in non-sterile soil.

Muhammad Fahyu Sanjaya, S.P., M.P

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Scopus Publications

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