Nurture B. Pharm young buddies more than 11 years and turned them into dynamic professionals and better human. I worked not only for organizational growth but believe in new, innovative and creative thought process. I am Expert in converting challenges into opportunities. I Fellow my passion with full enthusiasm.
EDUCATION
Pursuing PhD in Pharmaceutics and completed M.Pharm in Pharmaceutics.
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmaceutical Science, Pharmacology, Toxicology and Pharmaceutics, Multidisciplinary, Drug Discovery
5
Scopus Publications
Scopus Publications
32 Factorial Design of O/W Nanoemulsions of Cholecalciferol Using Box–Behnken Model for Plaque Psoriasis Mukesh Chandra Sharma, Mukul Sharma Indian Journal of Pharmaceutical Education and Research, 2023 Aim: The aim of the study was to design O/W Nanoemulsion using Cholecalciferol in different ratios of surfactants with Box-Behnken design model using Design Expert version 12. Response surface methodology, Box-Behnken was most suited followed by 3 independent variables (A, B, C) and 9 different responses with 17 different batches. Various responses like pH, % drug content drug entrapment and % in vitro drug release at different time intervals comparable with independent variables with % predicted errors calculated with SD±5 values. Materials and Methods: Cholecalciferol used as model drug, SPAN 20, SPAN 80 used as surfactant and Soybean oil used as oil phase for making Nanoemulsion preparation for plaque psoriasis treatment prepared by probe sonication method. Results: The VIF values were 1.0-1.03 and Ri 2 range between 0.00-0.0058 means correlated with each-others were in range, showed statistically correct and chances of error is minimum shown in Table 1. The predicted pH 7.79±0.38, Drug entrapment 92.68±4.67 and % Drug content was found to be 92.5±6.08 while experimental values were 7.20±0.11, 95.32±0.76, 93.29±1.15%. The predicted values of in vitro drug release were 5.54±1.57, 13.98±2.19, 25.96±4.40, 47.14±3.18, 68.17±6.02, 80.36±4.39% while experimental values were 8.50±0.50, 16.54±2.30, 29.54±2.27, 42.20±1.10, 68.54±1.27, 79.64±2.35% at 10, 30,60,90,120,210 min respectively see Table 2. The p values was <0.0001. Conclusion: The model showed F -values were in the range 0.28-5.08 showed non-significant while p -values was 0.028 showed significant values followed quadratic model. This showed that the model is best suitable for optimization.
Novel applications of Cold Atmospheric Plasma for the treatment of Plaque Psoriasis Mukesh Chandra Sharma, Mukul Sharma Research Journal of Pharmacy and Technology, 2023 Plasma is the fourth state of matter other than solid, liquid and gases. About 99 percent of mixture of free positive negative charges, neutral particles and photons. The plasma exists in two forms: hot plasma and cold plasma. Hot plasma is used in metallurgical operations while cold plasma is used in medical, skin psoriasis treatment, food agricultural, preservation and waste water treatment. Many clinical studies prove the effectiveness of cold plasma in dermatological disease management. Recent researches have established that combined use of cold plasma and nano-formulations is helpful in delivery of poorly water-soluble drugs. Plaque psoriasis is an autoimmune disorder in which silvery, thick, dead layer of skin shades off and cause inflammation, sometimes bleeding. It occurs in 3-4 percent of total world population. Scientific studies have proved that nano-scale particles permeate easily and interact with immune cells like lnterleukin-16, cytokines and TNF-alfa during inflammation and autoimmune disorders. As a result of which, thick layer of keratinocytes is produced and formation of TNF-alfa and interleukin is inhibited which are the causes of Plaque psoriasis. Since conventional medication has only been helpful in managing it, researches have been carried out using various techniques like phototherapy, use of UVA, UVB, IPL (Intense Pulsed Light), PDL (Pulsed Dye Light), LASER and low-level LASER light etc. but their excess exposure causes risk of skin cancer. Other therapies like use of nanometric Liposomes, Niosomes and their combination therapy are some novel clinical techniques for combating symptoms but are still in clinical trial. Since, Plasma therapy is non irritating and stops the triggering mechanism of inflammation, hence the use of nanotechnological topical formulation in combination with plasma therapy has the potential to become possible treatment of Plaque Psoriasis in future.
Chemical modification of Ciprofloxacin IV formulation to combat solubility and stability issues Mukul Sharma, G. N. Darwhekar, Jyoti Rathore Aip Conference Proceedings, 2020 Parenteral are sterile dosage form of therapeutic agent that are free of viable micro- organisms and they are injected through the skin or mucous membranes into the internal body compartments. Ciprofloxacin is a classic drug (Model drug) which is used in treating different kinds of severe bacterial infections. It is weakly basic and shows solubility only in acidic medium but it leads to irritation when injected. This problem can be solved by adding chemical modifiers like PEG-400 and PG at low concentration which would give 100% bioavailability with no toxicity and stable in pH 7.4. This study is performed to enhance solubility of Ciprofloxacin IV infusion by adding PEG-400 and PG have 75.33±0.57 and 98.66±0.52 mg/mL respectively. All formulations (F1-F9) were found to be sterile, clear, isotonic with blood plasma. Stability study showed that all formulations remains stable at temperature 40±2°C and 75±5% RH. Among all formulations F3 is best suited and passed all evaluation parameters.
The diverse applications of plasma Mukul Sharma, Shivani Dubey, Gajanan Darwhekar, Sudhir Kumar Jain Aip Conference Proceedings, 2015 Plasma being the fourth state of matter has always been an attraction for Physicists and Chemists. With the advent of time, plasma energy has been recognized in having widening horizons in the field of Biomedical Sciences. Plasma medicine can be subdivided into three main fields; Non-thermal atmospheric-pressure direct plasma for medical therapy; Plasma-assisted modification of bio-relevant surfaces and Plasma-based bio-decontamination and sterilization. The basis of the research is that as it has free carrier molecules, it has the ability to target specific cells and regulate functions like wound healing. Plasma does not harm healthy human cells but can kill bacteria and possibly even cancer cells to help treat various diseases. Nosocomial infection control, prevention and containment of contagious diseases, disinfection of medical devices, surface treatment (heat and UV sensitive surfaces) are research of interest. Recent success in generating plasma at very low temperature ie. Cold plasma makes the therapy painless. It has the ability to activate cellular responses and important mechanisms in the body. They target specific molecules such as prothrombin for blood coagulation, cytokines for killing bacteria, and angiogenesis for tissue regeneration. Plasma has bactericidal, fungicidal and virucidal properties. Plasma technology has flourishing future in diverse fields like Textiles, Nanofabrication, Automotives, Waste management, Microbiology, Food Hygiene, Medical Science like Skin treatments, sterilisation of wounds, Hand disinfection, Dental treatments etc. Food hygiene using plasma can be achieved in disinfection of food containers, food surface disinfection, hygiene in food handling, preparation and packaging. Therefore Plasma is most promising field for budding Scientist for fluorishing research in Biological Sciences.Plasma being the fourth state of matter has always been an attraction for Physicists and Chemists. With the advent of time, plasma energy has been recognized in having widening horizons in the field of Biomedical Sciences. Plasma medicine can be subdivided into three main fields; Non-thermal atmospheric-pressure direct plasma for medical therapy; Plasma-assisted modification of bio-relevant surfaces and Plasma-based bio-decontamination and sterilization. The basis of the research is that as it has free carrier molecules, it has the ability to target specific cells and regulate functions like wound healing. Plasma does not harm healthy human cells but can kill bacteria and possibly even cancer cells to help treat various diseases. Nosocomial infection control, prevention and containment of contagious diseases, disinfection of medical devices, surface treatment (heat and UV sensitive surfaces) are research of interest. Recent success in generating plasma at very low temperature ie. Cold plasma makes the the...
