Goncharova Natalia

@blood.by

laboratory of biology and genetics of stem cells
Republican Scientific and Practical Center of Transfusiology and Medical Biotechnology

https://researchid.co/n.goncharova

RESEARCH, TEACHING, or OTHER INTERESTS

Immunology, Biotechnology, Cell Biology, Multidisciplinary

Scopus Publications

387

Scholar Citations

Scholar h-index

Scholar i10-index

Scopus Publications

Comparison the impact of mesenchymal stromal cells, their microvesicles and plasma enriched with soluble platelet factors on survival and apoptosis of rat spleen lymphocytes in vitro
Oksana V. Klimenkova, Michael P. Potapnev, Natalia V. Goncharova, Oleg A. Kudelich, and Gennady G. Kondratenko
ECO-Vector LLC
BACKGROUND: The trophic function is one of the important but insufficiently studied features of mesenchymal stromal cells (MSCs), their microvesicles (MVs), and plasma enriched with soluble platelet factors (PRPs), which ensure the viability of target cells.AIM: To analyze the capability of MSCs, MVs, and PRP to affect the viability and spontaneous and activation-induced apoptosis of rat spleen lymphocytes during culturing in vitro.MATERIALS AND METHODS: MSCs were isolated from the mononuclear cell fraction of the femoral bone marrow of Wistar rats using the plastic adhesion method. MVs were obtained from the conditioned medium of MSCs by centrifugation at 14,500 g. PRP was prepared by freezing/thawing rat peripheral blood platelet concentrate. Rat spleen lymphocytes were isolated on a density gradient of 1.077 g/cm3. The viability and degree of lymphocyte apoptosis in vitro were assessed by flow cytometry for 7-AAD incorporation and binding to annexin V.RESULTS: The presence of MSCs at 10 and 20% concentrations caused an increase in the number of living intact and PMA-activated lymphocytes by the end of 3-day in vitro cultivation. Compared with controls, the amount of necrotic cells decreased 8.3–13.5 times, and the number of apoptotic cells decreased 2.3–4.0 times, mainly due to lymphocytes at the late stage of apoptosis. MVs of MSCs at the indicated concentrations did not significantly affect the viability of lymphocytes cultured in vitro but reduced the level of apoptosis of intact and PMA-activated lymphocytes by 3.6 (р=0.03) and 4.8–5.2 (р=0.048; р=0.03) times respectively. Studies have shown that 1.25% rat PRP has a growth-stimulatory activity against MSCs but not against lymphocytes cultured in vitro. In the culture of intact lymphocytes, PRPs did not significantly affect cell viability with a slight decrease (2.7–2.9 fold, p 0.05) in the number of necrotic cells. In the culture of PMA-activated lymphocytes, 1.25–2.50% PRP increased the number of living cells by 1.6–2.2 times (р=0.002; р=0.01) and the number of necrotic cells by two times (р=0.02).CONCLUSION: MSCs, MVs of MSCs, and PRP, in decreasing order, enhanced the viability of rat spleen lymphocytes and suppressed late apoptosis during in vitro cultivation. Lymphocytes activated by phorbol myristate acetate are more sensitive to their vital action compared with resting cells.

Comparative Evaluation of Cellular Origin Bioproducts Effects on the Course of Acute Necrotizing Pancreatitis in Experiment
О. А. Куделич, Г. Г. Кондратенко, Михаил Петрович Потапнев, Татьяна Сергеевна Колесникова, О. В. Клименкова and Надежда Вячеславовна Гончарова

Цель. Обосновать возможность коррекции системной патологической реакции при экспериментальном остром некротизирующем панкреатите с помощью локального применения мезенхимальных стромальных клеток (МСК), их микровезикул (МВ) или плазмы, обогащенной растворимыми факторами тромбоцитов (ПОРФТ). Материалы и методы. Изучено влияние МСК, МВ МСК и ПОРФТ на гематологические и биохимические показатели при остром экспериментальном некротизирующем панкреатите. Исследование проведено на 54 половозрелых крысах-самцах линии Wistar. Модель острого некротизирующего панкреатита создавали стандартно введением 0,3 мл 5% неионного детергента полиэтиленгликоль-октилфенолового эфира в хвостовую часть поджелудочной железы крыс. На 3-и и 7-е сутки от начала моделирования заболевания оценивали гематологические показатели, маркеры системного проявления патологического процесса (альфа-амилаза, глюкоза, аспартатаминотрансфераза, аланинаминотрансфераза), маркеры эндогенной интоксикации (малоновый диальдегид, уровень оксида азота), маркеры системного воспалительного ответа (фактор некроза опухоли α, интерлейкин-6, СРБ). Результаты. Применение МСК и их микровезикул на ранней стадии острого некротизирующего панкреатита положительно влияет на количество тромбоцитов в периферической крови экспериментальных животных. В эти же сроки МСК предотвращали гипергликемию, что может свидетельствовать о цитопрототекторном их действии в отношении гормонпродуцирующих клеток поджелудочной железы. Использование МСК и ПОРФТ способствует нормализации активности α-амилазы в сыворотке крови, что также может косвенно свидетельствовать о системном антиферментном, опосредованном через цитопротекторное действие в отношении экзокринных клеточных структур поджелудочной железы. Использование ПОРФТ, МСК и МВ МСК при остром экспериментальном некротизирующем панкреатите уменьшает системный воспалительный ответ, что продемонстрировала динамика значений ФНО-α и ИЛ-6. В сыворотке крови животных, которым проводилось лечение МСК, уровень СРБ на протяжении всего эксперимента не повышался. Регионарное использование МСК препятствует росту уровня оксида азота и малонового диальдегида в сыворотке крови экспериментальных животных. Заключение. Раннее регионарное применение биопродуктов клеточного происхождения при экспериментальном остром некротизирующем панкреатите оказывает системный положительный эффект, что позволяет рассматривать их в качестве перспективных средств для патогенетического лечения данного заболевания. Purpose. To substantiate a possibility of correcting a systemic pathological reaction in experimental acute necrotizing pancreatitis by local application of mesenchymal stromal cells (MSCs), their microvesicles (MV MSCs), or plasma enriched with platelet factors (PRP). Materials and methods. The effect of MSCs, MB MSCs and PORFT on hematological and biochemical parameters in acute experimental necrotizing pancreatitis was studied. The study was carried out on 54 adults Wistar rats. Acute pancreatitis was induced by introducing 0.3 ml of 5% solution of non-ionic polyethylene glycol octylphenol ether detergent into the caudal part of rats’ pancreas. Hematological parameters, markers of systemic manifestation of the pathological process (alpha-amylase, glucose, AST, ALT), markers of endogenous intoxication (malondialdehyde, nitric oxide), and markers of systemic inflammatory response (tumor necrosis factor-alpha, interleukin-6, CRP) were evaluated on the 3rd and 7th day from the beginning of disease modeling. Results. MSCs and their microvesicles used at early stages of acute necrotizing positively affect the number of platelets in the peripheral blood of experimental animals. At the same time, MSCs prevented hyperglycemia, indicating their cytoprotective effect on hormone-producing pancreatic cells. The use of MSCs and PRP promotes normalization of α-amylase activity in serum, which may also indirectly indicate systemic anti-enzyme action, mediated through cytoprotective one, against exocrine cellular structures of the pancreas. MSCs, MB MSCs and PRP application in acute experimental necrotizing pancreatitis reduced the systemic inflammatory response, as demonstrated by TNF-α and IL-6 values changes. CRP blood serum levels in animals treated with MSCs did not increase throughout the experiment. A regional use of MSCs prevents increasing nitric oxide and malondialdehyde levels in blood serum of experimental animals. Conclusion. A regional use of cell origin bioproducts at early stages of experimental acute necrotizing pancreatitis provides a positive systemic effect, which allows considering them as promising agents for pathogenetic treatment of this disease.

Regional use of extracellular microvesicles of mesenchymal stromal cells in acute necrotizing pancreatitis in an experiment
O. A. Kudelich, G. G. Kondratenko, M. P. Potapnev, O. V. Klimenkova, and N. V. Goncharova
IPO Association of Transplantologists
Background. The significance of the problem of treatment of acute pancreatitis is due to an increase in the incidence with an increase in the number of necrotizing forms, accompanied by a high incidence of severe complications and high mortality.Objective. To identify the impact of regionally used extracellular microvesicles of mesenchymal stromal cells on the endogenous intoxication markers in acute necrotizing pancreatitis in the experiment.Material and methods. Acute pancreatitis was induced by the introduction of a 0.3 ml of 5% solution of non-ionic polyethylene glycol octylphenol ether detergent into the caudal part of the rat pancreas. The study was conducted on 42 adult Wistar rats, which were randomly divided into 4 groups. Group I (n=6) included intact animals, Group II (control group) (n=12) included rats with pancreatitis without treatment, Group III (n=12) consisted of rats with pancreatitis treated with analgesia + infusions of 0.9% sodium chloride solution (saline), Group IV (n=12) included rats with pancreatitis treated with analgesia+ saline infusions + regional application of extracellular microvesicles of mesenchymal stromal cells. Cells were obtained from the bone marrow of healthy animals. Microvesicles were obtained by differential centrifugation under sterile conditions. Microvesicles were administered one day after the pancreatitis induction through the catheter installed into the pathologically altered part of the pancreas. The dose of microvesicles was calculated as equivalent to (derived from) 1 million mesenchymal stromal cells. The hematological parameters, markers of the systemic manifestation of the pathological process (alpha-amylase, aspartate aminotransferase, alanine aminotransferase), the endogenous intoxication markers (lipid peroxidation activity, nitric oxide level), the systemic inflammatory response markers (tumor necrosis factor-alpha, interleukin-6) were studied on the 3rd and 7th day from the start of disease modeling.Results. Regional use of extracellular microvesicles of mesenchymal stromal cells in the treatment of acute experimental necrotizing pancreatitis at an early stage helped to normalize the level of blood platelets, reduce enzymeemia, elements of endogenous intoxication (interleukin-6, tumor necrosis factor-alpha), and the nitric oxide level.Conclusion. The early application of extracellular microvesicles of mesenchymal stromal cells in the treatment of acute necrotizing pancreatitis in an experiment has a positive effect on parameters, which are key links of pathogenesis and leading markers of this disease severity.

Dual Effect of Amino Acid Compositions on Antibacterial Activity of Human Neutrophilic Granulocytes
M. P. Potapnev, S. V. Andreyev, N. V. Goncharova, O. I. Viatkina, E. L. Berdina, and V. N. Gapanovich
Pleiades Publishing Ltd

Dual effect of amino acid compositions upon antibacterial activity of human neutrophilic granulocytes
M.P. Potapnev, , S.V. Andreyev, N.V. Goncharova, O.I. Viatkina, E.L. Berdina, V.N. Gapanovich, , , ,et al.
Geotar-Media Publishing Group

Clinical benefits of single vs repeated courses of mesenchymal stem cell therapy in epilepsy patients
Fedor Hlebokazov, Tatiana Dakukina, Michael Potapnev, Svetlana Kosmacheva, Lubov Moroz, Nikolai Misiuk, Tatiana Golubeva, Elena Slobina, Olga Krasko, Antos Shakhbazau,et al.
Elsevier BV

Treatment of refractory epilepsy patients with autologous mesenchymal stem cells reduces seizure frequency: An open label study
Fedor Hlebokazov, Tatiana Dakukina, Svetlana Ihnatsenko, Svetlana Kosmacheva, Michael Potapnev, Antos Shakhbazau, Natalia Goncharova, Michael Makhrov, Pavel Korolevich, Nikolai Misyuk,et al.
Elsevier BV

The Use of Autologous Mesenchymal Stem Cells for Cell Therapy of Patients with Amyotrophic Lateral Sclerosis in Belarus
Yu. N. Rushkevich, S. M. Kosmacheva, G. V. Zabrodets, S. I. Ignatenko, N. V. Goncharova, I. N. Severin, S. A. Likhachev, and M. P. Potapnev
Springer Science and Business Media LLC

Non-viral engineering of skin precursor-derived Schwann cells for enhanced NT-3 production in adherent and microcarrier culture
A. Shakhbazau, D. Shcharbin, M. Bryszewska, R. Kumar, H. M. Wobma, M. S. Kallos, N. Goncharova, I. Seviaryn, S. Kosmacheva, M. Potapnev,et al.
Bentham Science Publishers Ltd.
Genetic engineering of stem cells and their derivatives has the potential to enhance their regenerative capabilities. Here, dendrimer- and lipofection-based approaches were used for non-viral neurotrophin-3 (NT-3) over-expression in Schwann cells differentiated from skin precursors (SKP-SCs). A variety of dendrimers were first tested for transfection efficiency on HEK 293T cells, with PAMAMNH2 G4 found most effective and used subsequently for SKP-SCs transfection. Plasmid-based expression resulted in increased NT-3 release from SKP-SCs in both adherent and microcarrier-based culture. In a proof-of-concept study, the microcarrier/SKP-SCs were implanted into the injured nerve, and transfected cells were shown to detach, integrate into the nerve tissue and associate with regenerating axons. Virus-free systems for transient neurotrophin expression are a feasible and biologically safe option to increase the therapeutic value of stem cells and stem cell-derived cells in nerve repair strategies. Further work to develop bioprocesses for expansion of SKP-SCs on microcarriers in bioreactors is still needed.

In vitro expansion and lineage commitment of the human umbilical cord blood myeloid progenitors

Dendrimer-driven neurotrophin expression differs in temporal patterns between rodent and human stem cells
Antos Shakhbazau, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, Svetlana Kosmacheva, Mihail Potapnev, Maria Bryszewska, Ranjan Kumar, Jeffrey Biernaskie, and Rajiv Midha
American Chemical Society (ACS)
This study reports the use of a nonviral expression system based on polyamidoamine dendrimers for time-restricted neurotrophin overproduction in mesenchymal stem cells and skin precursor-derived Schwann cells. The dendrimers were used to deliver plasmids for brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) expression in both rodent and human stem cells, and the timelines of expression were studied. We have found that, despite the fact that transfection efficiencies and protein expression levels were comparable, dendrimer-driven expression in human mesenchymal stem cells was characterized by a more rapid decline compared to rodent cells. Transient expression systems can be beneficial for some neurotrophins, which were earlier reported to cause unwanted side effects in virus-based long-term expression models. Nonviral neurotrophin expression is a biologically safe and accessible alternative to increase the therapeutic potential of autologous adult stem cells and stem cell-derived functional differentiated cells.

Non-virally modified human mesenchymal stem cells produce ciliary neurotrophic factor in biodegradable fibrin-based 3D scaffolds
Antos Shakhbazau, Dzmitry Shcharbin, Natalya Petyovka, Natalya Goncharova, Ihar Seviaryn, Svetlana Kosmacheva, Maria Bryszewska, and Mihail Potapnev
Elsevier BV
We report the adaptation of dendrimer-based nonviral expression system for ciliary neurotrophic factor (CNTF) overproduction in human mesenchymal stem cells (hMSCs) embedded into fibrin-based three-dimensional (3D) matrix. Time-restricted neurotrophin expression enables autologous adult stem cells for additional trophic support and increases their therapeutic potential in neuroregeneration applications. Polyamidoamine (PAMAM)-NH(2) dendrimers of fourth generation effectively provided virus-free delivery and expression of CNTF-internal ribosome entry site-green fluorescent protein cassette with a transfection efficiency in hMSCs over 11%. CNTF levels in transfected cultures were 10-fold higher as compared with the control cells. Dendrimer-driven CNTF expression also persisted in hMSCs embedded into fibrin-based 3D matrix, an emerging vehicle for cell delivery or bioartificial organ formation. Nonviral modification of autologous adult stem cells with use of dendrimers is a novel tool perspective in terms of biosafety and technological availability.

Fourth generation phosphorus-containing dendrimers: Prospective drug and gene delivery carrier
D. Shcharbin, V. Dzmitruk, A. Shakhbazau, N. Goncharova, I. Seviaryn, S. Kosmacheva, M. Potapnev, E. Pedziwiatr-Werbicka, M. Bryszewska, M. Talabaev,et al.
MDPI AG
Research concerning new targeting delivery systems for pharmacologically active molecules and genetic material is of great importance. The aim of the present study was to investigate the potential of fourth generation (P4) cationic phosphorus-containing dendrimers to bind fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS), anti-neoplastic drug cisplatin, anti-HIV siRNA siP24 and its capability to deliver green fluorescent protein gene (pGFP) into cells. The interaction between P4 and ANS (as the model drug) was investigated. The binding constant and the number of binding centers per one molecule of P4 were determined. In addition, the dendriplex between P4 and anti-HIV siRNA siP24 was characterized using circular dichroism, fluorescence polarization and zeta-potential methods; the average hydrodynamic diameter of the dendriplex was calculated using zeta-size measurements. The efficiency of transfection of pGFP using P4 was determined in HEK293 cells and human mesenchymal stem cells, and the cytotoxicity of the P4-pGFP dendriplex was studied. Furthermore, enhancement of the toxic action of the anti-neoplastic drug cisplatin by P4 dendrimers was estimated. Based on the results, the fourth generation cationic phosphorus-containing dendrimers seem to be a good drug and gene delivery carrier candidate.

Effect of cholesterol on the functional activity of proteins responsible for the resistance of human lymphocytes to xenobiotics
A. V. Tamashevski, N. M. Kozlova, N. V. Goncharova, G. P. Zubritskaya, and E. I. Slobozhanina
Pleiades Publishing Ltd

[Effect of cholesterol on the functional activity of proteins responsible for the resistance of human lymphocytes to xenobiotics].

Hepatogenic potential of human bone marrow and umbilical cord blood mesenchymal stem cells
S. M. Kosmacheva, I. N. Seviaryn, N. V. Goncharova, N. V. Petyovka, and M. P. Potapnev
Springer Science and Business Media LLC

Neurons and stromal stem cells as targets for polycation-mediated transfection
A. V. Shakhbazau, D. G. Shcharbin, N. V. Goncharova, I. N. Seviaryn, S. M. Kosmacheva, N. A. Kartel, M. Bryszewska, J. P. Majoral, and M. P. Potapnev
Springer Science and Business Media LLC

Revealing some virus infections by lymphocyte laser scattering
Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, and Valery A. Loiko
SPIE
Light scattering patterns have been calculated for a simple optical model constructed for single smooth and nonsmooth lymphocytes of healthy and infected individuals. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward and backward scattered light. We have found by calculations and validated by the flow-cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flowcytometry detecting of individuals infected with viruses of hepatitises B or C and some others viruses are presented.

Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals
Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, and Valery A. Loiko
SPIE-Intl Soc Optical Eng
A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitis B or C and some others viruses are presented.

Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells
Antos Shakhbazau, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, Svetlana Kosmacheva, Mihail Potapnev, Barbara Gabara, Maxim Ionov, and Maria Bryszewska
Springer Science and Business Media LLC

Transfection efficiencies of PAMAM dendrimers correlate inversely with their hydrophobicity
Antos Shakhbazau, Iauhenia Isayenka, Nikolai Kartel, Natalya Goncharova, Ihar Seviaryn, Svetlana Kosmacheva, Mihail Potapnev, Dzmitry Shcharbin, and Maria Bryszewska
Elsevier BV

Erratum: "Plasticity of human mesenchymal stem cell phenotype and expression profile under neurogenic conditions" (Bulletin of Experimental Biology and Medicine (2009) vol. 147(4) (513-516))
A. V. Shakhbazau, N. V. Goncharova, S. M. Kosmacheva, N. A. Kartel’, and M. P. Potapnev
Springer Science and Business Media LLC

Plasticity of human mesenchymal stem cell phenotype and expression profile under neurogenic conditions
A. V. Shakhbazov, N. V. Goncharova, S. M. Kosmacheva, N. A. Kartel’, and M. P. Potapnev
Springer Science and Business Media LLC

Viral vectors for stable transduction of human mesenchymal stem cells: Systems based on adeno-associated viruses and lentiviruses
A. V. Shakhbazau, I. N. Sevyaryn, N. V. Goncharova, V. V. Grinev, S. M. Kosmacheva, and M. P. Potapnev
Springer Science and Business Media LLC

Development of human granulocyte morphometric model for scanning flow cytometry
Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, and Valery A. Loiko
SPIE
Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.

RECENT SCHOLAR PUBLICATIONS

Dual Effect of Amino Acid Compositions on Antibacterial Activity of Human Neutrophilic Granulocytes
MP Potapnev, SV Andreyev, NV Goncharova, OI Viatkina, EL Berdina, ...
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry 17 (1), 17-25 2023

Sistemnoe vs lokal'noe vvedenie mezenkhimal'nykh stromal'nykh kletok dlya terapii neyrodegenerativnykh zabolevaniy cheloveka
MP Potapnev, SM Kosmacheva, LA Repina, AG Ionova, NV Goncharova, ...
Genes & Cells 17 (3), 189-190 2022

MARKERS OF PLATELET DYSFUNCTION IN THE FIRST 48 HOURS OF LACUNAR STROKE RESULTING FROM CEREBRAL SMALL VESSEL DISEASE
L Anatskaia, N Goncharova, L Matusevich, N Shcharbina, V Zabarovski, ...
INTERNATIONAL JOURNAL OF STROKE 16 (2_ SUPPL), 50-50 2021

Clinical benefits of single vs repeated courses of mesenchymal stem cell therapy in epilepsy patients
F Hlebokazov, T Dakukina, M Potapnev, S Kosmacheva, L Moroz, ...
Clinical Neurology and Neurosurgery 207, 106736 2021

Circulating cytological markers of endothelial dysfunction in acute lacunar stroke resulting from cerebral small vessels disease
L Anatskaia, N Goncharova, N Shcharbina
Journal of the Neurological Sciences 381, 393 2017

Mezenkhimnye stromal'nye kletki platsenty cheloveka podderzhivayut differentsirovku gemopoeticheskikh CD34+ kletok pupovinnoy krovi v granulotsitarno-monotsitarnom napravlenii
EV Vasina, VS Kostyunina, NV Goncharova, IN Severin, NV Petevka
Genes & Cells 12 (3), 60-60 2017

The features of cellular immunity in patients with treatment-resistant asthma
Z Antanovich, N Goncharova
European Respiratory Journal 50 (suppl 61) 2017

Treatment of refractory epilepsy patients with autologous mesenchymal stem cells reduces seizure frequency: an open label study
F Hlebokazov, T Dakukina, S Ihnatsenko, S Kosmacheva, M Potapnev, ...
Advances in medical sciences 62 (2), 273-279 2017

P-GLYCOPROTEIN TRANSPORT ACTIVITY UNDER REDOX BALANCE CHANGES IN LYMPHOCYTES OF PATIENTS WITH B-CHRONIC LYMPHOCYTIC LEUKEMIA
Y Harmaza, A Tamashevski, N Goncharova, E Slobozhanina, ...
HAEMATOLOGICA 101, 721-721 2016

Our experience in application of autologous bone-marrow-derived mesenchymal stem cells for therapy of patients with symptomatic epilepsy
FP Hlebokazov, TV Dоkukina, SI Ighnatenko, SM Кosmacheva, ...
Epilepsy and paroxysmal conditions 6 (1), 6-14 2016

Growth-stimulating activity of platelet preparations in relation to mesenchymal stem cells in vitro
SI Ihnatsenko, SM Kasmachova, MP Potapnev, EA Kahno, ...
Proceedings of the National Academy of Sciences of Belarus, Medical series 2016

Clinical phenotype of destructive-regenerative endothelial response in the first 48 hours of lacunar stroke resulting from cerebral small vessels disease
L Anatskain, N Goncharova, G Khulup
CEREBROVASCULAR DISEASES 41, 198-198 2016

EFFICIENCY OF MONTELUKAST TREATMENT OF THE CHILDREN, SUFFERING FROM BRONCHIAL ASTHMA
II Balabolkin, IE Smirnov, AV Liapunov, OF Lukina, NV Goncharova, ...
Current Pediatrics 5 (5), 35-38 2015

The content of natural killer cells in the blood of patients with asthma in combination with depression
Z Antanovich, N Goncharova
European Respiratory Journal 46 (suppl 59) 2015

The use of autologous mesenchymal stem cells for cell therapy of patients with amyotrophic lateral sclerosis in belarus
YN Rushkevich, SM Kosmacheva, GV Zabrodets, SI Ignatenko, ...
Bulletin of experimental biology and medicine 159 (4), 576-581 2015

Effect of intravenous low intensity laser irradiation of blood on the level of angiogenic T-cells in acute lacunar stroke
L Anatskaia, N Goncharova, I Seviaryn, GG Khulup, SS Marchenko, ...
EUROPEAN JOURNAL OF NEUROLOGY 22, 808-808 2015

Mononuclears size-distribution as marker of acute leukemia
GI Ruban, NV Goncharova, DV Marinitch, VA Loiko
International Journal of Advance in Medical Science 3, 1-12 2015

Influence of intravenous low-intensity laser irradiation of blood on the level of circulating endothelial progenitor cells in acute lacunar stroke
L Anatskaia, N Goncharova, I Seviaryn, G Khulup
INTERNATIONAL JOURNAL OF STROKE 10, 367-367 2015

Influence of intravenous laser irradiation of blood on the levels of circulating endothelial progenitor cells and endothelial stem cells in the acute lacunar stroke
LN Anatskaia, NV Goncharova, IM Seviaryn, GY Khulup, SV Marchenko
Proceedings of the National Academy of Sciences of Belarus, Medical series 2015

MOST CITED SCHOLAR PUBLICATIONS

Investigation of morphometric parameters<? xpp qa?> for granulocytes and lymphocytes as applied<? xpp qa?> to a solution of direct and inverse light-scattering problems
GI Ruban, SM Kosmacheva, NV Goncharova, DV Bockstaele, VA Loiko
Journal of biomedical optics 12 (4), 044017-044017-11 2007
Citations: 58

Morphometric model of lymphocyte as applied to scanning flow cytometry
VA Loiko, GI Ruban, OA Gritsai, AD Gruzdev, SM Kosmacheva, ...
Journal of Quantitative Spectroscopy and Radiative Transfer 102 (1), 73-84 2006
Citations: 48

Plasticity of human mesenchymal stem cell phenotype and expression profile under neurogenic conditions
AV Shakhbazov, NV Goncharova, SM Kosmacheva, NA Kartel’, ...
Bulletin of experimental biology and medicine 147, 513-516 2009
Citations: 29

Neurons and stromal stem cells as targets for polycation-mediated transfection
AV Shakhbazau, DG Shcharbin, NV Goncharova, IN Seviaryn, ...
Bulletin of experimental biology and medicine 151, 126-129 2011
Citations: 25

Hepatogenic potential of human bone marrow and umbilical cord blood mesenchymal stem cells
SM Kosmacheva, IN Seviaryn, NV Goncharova, NV Petyovka, ...
Bulletin of experimental biology and medicine 151 (1), 142 2011
Citations: 22

Light scattering and morphology of the lymphocyte as applied to flow cytometry for distinguishing healthy and infected individuals
GI Ruban, VV Berdnik, DV Marinitch, NV Goncharova, VA Loiko
Journal of biomedical optics 15 (5), 057008-057008-9 2010
Citations: 22

Viral vectors for stable transduction of human mesenchymal stem cells: systems based on adeno-associated viruses and lentiviruses
AV Shakhbazau, IN Sevyaryn, NV Goncharova, VV Grinev, ...
Bulletin of experimental biology and medicine 146 (4), 531 2008
Citations: 17

Mononuclear cells morphology for cells discrimination by the angular structure of scattered light
VA Loiko, GI Ruban, OA Gritsai, VV Berdnik, NV Goncharova
Tenth Conference on Electromagnetic and Light Scattering 2007
Citations: 11

In vitro expansion and lineage commitment of the human umbilical cord blood myeloid progenitors
NV Petyovka, NV Goncharova, IM Seviaryn, SM Kosmacheva, ...
Genes & Cells 7 (1), 40-48 2012
Citations: 4

Investigation of human white blood cells: application to scanning flow cytometry
VA Loiko, GI Ruban, OA Gritsai, AA Miskevich, SM Kosmacheva, ...
International Conference on Lasers, Applications, and Technologies 2005 2006
Citations: 2

Discrepancy between direct and antibody-dependent cytotoxic activities of human LAK cells
MP Potapnev, TS Garbuzenco, NV Goncharova, VD Zobnin, OV Shadrin, ...
Immunology letters 41 (1), 13-17 1994
Citations: 2

Mononuclears size-distribution as marker of acute leukemia
GI Ruban, NV Goncharova, DV Marinitch, VA Loiko
International Journal of Advance in Medical Science 3, 1-12 2015
Citations: 1

Effect of cholesterol on the functional activity of proteins responsible for the resistance of human lymphocytes to xenobiotics
AV Tamashevski, NM Kozlova, NV Goncharova, GP Zubritskaya, ...
Biophysics 56, 436-443 2011
Citations: 1

Development of human granulocyte morphometric model for scanning flow cytometry
GI Ruban, SM Kosmacheva, NV Goncharova, VA Loiko
International Conference on Lasers, Applications, and Technologies 2007 2007
Citations: 1

Elaboration of optical model of lymphocytes as applied to scanning flow cytometry
VA Loiko, GI Ruban, OA Gritsai, SM Kosmacheva, NV Goncharova
9th International Conference, 159 2006
Citations: 1