Goncharova Natalia
@blood.by
laboratory of biology and genetics of stem cells
Republican Scientific and Practical Center of Transfusiology and Medical Biotechnology
RESEARCH, TEACHING, or OTHER INTERESTS
Immunology, Biotechnology, Cell Biology, Multidisciplinary
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
M. P. Potapnev, S. V. Andreyev, N. V. Goncharova, O. I. Viatkina, E. L. Berdina, and V. N. Gapanovich
Pleiades Publishing Ltd
M.P. Potapnev, , S.V. Andreyev, N.V. Goncharova, O.I. Viatkina, E.L. Berdina, V.N. Gapanovich, , , ,et al.
Geotar-Media Publishing Group
Fedor Hlebokazov, Tatiana Dakukina, Michael Potapnev, Svetlana Kosmacheva, Lubov Moroz, Nikolai Misiuk, Tatiana Golubeva, Elena Slobina, Olga Krasko, Antos Shakhbazau,et al.
Elsevier BV
Fedor Hlebokazov, Tatiana Dakukina, Svetlana Ihnatsenko, Svetlana Kosmacheva, Michael Potapnev, Antos Shakhbazau, Natalia Goncharova, Michael Makhrov, Pavel Korolevich, Nikolai Misyuk,et al.
Elsevier BV
Yu. N. Rushkevich, S. M. Kosmacheva, G. V. Zabrodets, S. I. Ignatenko, N. V. Goncharova, I. N. Severin, S. A. Likhachev, and M. P. Potapnev
Springer Science and Business Media LLC
A. Shakhbazau, D. Shcharbin, M. Bryszewska, R. Kumar, H. M. Wobma, M. S. Kallos, N. Goncharova, I. Seviaryn, S. Kosmacheva, M. Potapnev,et al.
Bentham Science Publishers Ltd.
Genetic engineering of stem cells and their derivatives has the potential to enhance their regenerative capabilities. Here, dendrimer- and lipofection-based approaches were used for non-viral neurotrophin-3 (NT-3) over-expression in Schwann cells differentiated from skin precursors (SKP-SCs). A variety of dendrimers were first tested for transfection efficiency on HEK 293T cells, with PAMAMNH2 G4 found most effective and used subsequently for SKP-SCs transfection. Plasmid-based expression resulted in increased NT-3 release from SKP-SCs in both adherent and microcarrier-based culture. In a proof-of-concept study, the microcarrier/SKP-SCs were implanted into the injured nerve, and transfected cells were shown to detach, integrate into the nerve tissue and associate with regenerating axons. Virus-free systems for transient neurotrophin expression are a feasible and biologically safe option to increase the therapeutic value of stem cells and stem cell-derived cells in nerve repair strategies. Further work to develop bioprocesses for expansion of SKP-SCs on microcarriers in bioreactors is still needed.
Antos Shakhbazau, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, Svetlana Kosmacheva, Mihail Potapnev, Maria Bryszewska, Ranjan Kumar, Jeffrey Biernaskie, and Rajiv Midha
American Chemical Society (ACS)
This study reports the use of a nonviral expression system based on polyamidoamine dendrimers for time-restricted neurotrophin overproduction in mesenchymal stem cells and skin precursor-derived Schwann cells. The dendrimers were used to deliver plasmids for brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) expression in both rodent and human stem cells, and the timelines of expression were studied. We have found that, despite the fact that transfection efficiencies and protein expression levels were comparable, dendrimer-driven expression in human mesenchymal stem cells was characterized by a more rapid decline compared to rodent cells. Transient expression systems can be beneficial for some neurotrophins, which were earlier reported to cause unwanted side effects in virus-based long-term expression models. Nonviral neurotrophin expression is a biologically safe and accessible alternative to increase the therapeutic potential of autologous adult stem cells and stem cell-derived functional differentiated cells.
Antos Shakhbazau, Dzmitry Shcharbin, Natalya Petyovka, Natalya Goncharova, Ihar Seviaryn, Svetlana Kosmacheva, Maria Bryszewska, and Mihail Potapnev
Elsevier BV
We report the adaptation of dendrimer-based nonviral expression system for ciliary neurotrophic factor (CNTF) overproduction in human mesenchymal stem cells (hMSCs) embedded into fibrin-based three-dimensional (3D) matrix. Time-restricted neurotrophin expression enables autologous adult stem cells for additional trophic support and increases their therapeutic potential in neuroregeneration applications. Polyamidoamine (PAMAM)-NH(2) dendrimers of fourth generation effectively provided virus-free delivery and expression of CNTF-internal ribosome entry site-green fluorescent protein cassette with a transfection efficiency in hMSCs over 11%. CNTF levels in transfected cultures were 10-fold higher as compared with the control cells. Dendrimer-driven CNTF expression also persisted in hMSCs embedded into fibrin-based 3D matrix, an emerging vehicle for cell delivery or bioartificial organ formation. Nonviral modification of autologous adult stem cells with use of dendrimers is a novel tool perspective in terms of biosafety and technological availability.
D. Shcharbin, V. Dzmitruk, A. Shakhbazau, N. Goncharova, I. Seviaryn, S. Kosmacheva, M. Potapnev, E. Pedziwiatr-Werbicka, M. Bryszewska, M. Talabaev,et al.
MDPI AG
Research concerning new targeting delivery systems for pharmacologically active molecules and genetic material is of great importance. The aim of the present study was to investigate the potential of fourth generation (P4) cationic phosphorus-containing dendrimers to bind fluorescent probe 8-anilino-1-naphthalenesulfonate (ANS), anti-neoplastic drug cisplatin, anti-HIV siRNA siP24 and its capability to deliver green fluorescent protein gene (pGFP) into cells. The interaction between P4 and ANS (as the model drug) was investigated. The binding constant and the number of binding centers per one molecule of P4 were determined. In addition, the dendriplex between P4 and anti-HIV siRNA siP24 was characterized using circular dichroism, fluorescence polarization and zeta-potential methods; the average hydrodynamic diameter of the dendriplex was calculated using zeta-size measurements. The efficiency of transfection of pGFP using P4 was determined in HEK293 cells and human mesenchymal stem cells, and the cytotoxicity of the P4-pGFP dendriplex was studied. Furthermore, enhancement of the toxic action of the anti-neoplastic drug cisplatin by P4 dendrimers was estimated. Based on the results, the fourth generation cationic phosphorus-containing dendrimers seem to be a good drug and gene delivery carrier candidate.
A. V. Tamashevski, N. M. Kozlova, N. V. Goncharova, G. P. Zubritskaya, and E. I. Slobozhanina
Pleiades Publishing Ltd
S. M. Kosmacheva, I. N. Seviaryn, N. V. Goncharova, N. V. Petyovka, and M. P. Potapnev
Springer Science and Business Media LLC
A. V. Shakhbazau, D. G. Shcharbin, N. V. Goncharova, I. N. Seviaryn, S. M. Kosmacheva, N. A. Kartel, M. Bryszewska, J. P. Majoral, and M. P. Potapnev
Springer Science and Business Media LLC
Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, and Valery A. Loiko
SPIE
Light scattering patterns have been calculated for a simple optical model constructed for single smooth and nonsmooth lymphocytes of healthy and infected individuals. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward and backward scattered light. We have found by calculations and validated by the flow-cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flowcytometry detecting of individuals infected with viruses of hepatitises B or C and some others viruses are presented.
Gennady I. Ruban, Vladimir V. Berdnik, Dmitry V. Marinitch, Natalia V. Goncharova, and Valery A. Loiko
SPIE-Intl Soc Optical Eng
A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitis B or C and some others viruses are presented.
Antos Shakhbazau, Dzmitry Shcharbin, Ihar Seviaryn, Natalya Goncharova, Svetlana Kosmacheva, Mihail Potapnev, Barbara Gabara, Maxim Ionov, and Maria Bryszewska
Springer Science and Business Media LLC
Antos Shakhbazau, Iauhenia Isayenka, Nikolai Kartel, Natalya Goncharova, Ihar Seviaryn, Svetlana Kosmacheva, Mihail Potapnev, Dzmitry Shcharbin, and Maria Bryszewska
Elsevier BV
A. V. Shakhbazau, N. V. Goncharova, S. M. Kosmacheva, N. A. Kartel’, and M. P. Potapnev
Springer Science and Business Media LLC
A. V. Shakhbazov, N. V. Goncharova, S. M. Kosmacheva, N. A. Kartel’, and M. P. Potapnev
Springer Science and Business Media LLC
A. V. Shakhbazau, I. N. Sevyaryn, N. V. Goncharova, V. V. Grinev, S. M. Kosmacheva, and M. P. Potapnev
Springer Science and Business Media LLC
Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, and Valery A. Loiko
SPIE
Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing with a costly and time-consuming immunophenotyping of the cells.
Gennady I. Ruban, Svetlana M. Kosmacheva, Natalia V. Goncharova, Dirk Van Bockstaele, and Valery A. Loiko
SPIE-Intl Soc Optical Eng
Quantitative data on cell structure, shape, and size distribution are obtained by optical measurement of normal peripheral blood granulocytes and lymphocytes in a cell suspension. The cell nuclei are measured in situ. The distribution laws of the cell and nuclei sizes are estimated. The data gained are synthesized to construct morphometric models of a segmented neutrophilic granulocyte and a lymphocyte. Models of interrelation between the cell and nucleus metric characteristics for granulocyte and lymphocyte are obtained. The discovered interrelation decreases the amount of cell-nucleus size combinations that have to be considered under simulation of cell scattering patterns. It allows faster analysis of light scattering to discriminate cells in a real-time scale. Our morphometric data meet the requirements of scanning flow cytometry dealing with the high rate analysis of cells in suspension. Our findings can be used as input parameters for the solution of the direct and inverse light-scattering problems in scanning flow cytometry, dispensing with a costly and time-consuming immunophenotyping of the cells, as well as in turbidimetry and nephelometry. The cell models developed can ensure better interpretations of scattering patterns for an improvement of discriminating capabilities of immunophenotyping-free scanning flow cytometry.
Valery A. Loiko, Gennady I. Ruban, Olga A. Gritsai, Alexander A. Miskevich, Svetlana M. Kosmacheva, and Natalia V. Goncharova
SPIE
Normal human leukocytes have been investigated in suspension by methods of the specialized light microscopy. On the basis of the obtained experimental data the histograms of the size distributions of lymphocytes, their nuclei, and granulocytes are constructed.
Valery A. Loiko, Gennady I. Ruban, Olga A. Gritsai, Alexey D. Gruzdev, Svetlana M. Kosmacheva, Natalia V. Goncharova, and Alexander A. Miskevich
Elsevier BV