Phytosome suspension of Bryonia laciniosa Linn. mitigates alcohol-induced liver cirrhosis and male infertility in wistar rats Abhijeet Patil, Namit Kudatarkar Journal of Biologically Active Products from Nature, 2025 Chronic alcohol intake is a major contributor to liver cirrhosis and is often associated with male infertility, primarily due to oxidative stress and hormonal dysregulation. Bryonia laciniosa Linn., a plant rich in flavonoids and saponins, has shown promise in mitigating hepatic and reproductive impairments. This study investigated the therapeutic potential of a phytosome suspension of B. laciniosa in addressing alcohol-induced liver cirrhosis and male infertility in Wistar rats. A hydro-methanolic extract was formulated into a phytosome using the thin-film hydration method and optimized via Central Composite Design. Male rats were subjected to 21-day chronic ethanol exposure, followed by treatment with either crude extract (100, 200, 400 mg/kg), phytosome suspension (200 mg/kg) or silymarin (100 mg/kg). Biochemical assays, antioxidant enzyme levels (SOD, GSH, MDA), sperm parameters, organ weights and histopathological evaluations were conducted. Alcohol exposure caused significant hepatic enzyme elevation, oxidative stress and spermatogenic damage. Treatment with the phytosome suspension notably improved hepatic and testicular parameters, with significant restoration in histoarchitecture and antioxidant defences (p < 0.001). Compared to crude extract, phytosome suspension demonstrated superior efficacy, likely due to improved bioavailability. These findings support the use of B. laciniosa phytosomes as a novel, dual-protective phytopharmaceutical strategy against alcohol-induced organ toxicity.
In silico screening and experimental validation of Carissa carandas L. extract against cyclophosphamide-induced cardiotoxicity in Wistar rats Roopadevi Desaipatti, Namit Kudatarkar, Sachin Gudasi Journal of Applied Pharmaceutical Science, 2025 The aim of this study was to evaluate the cardioprotective effects of Carissa carandas L extract against cardiotoxicity which was induced by cyclophosphamide (CP) in male Wistar rats. The target proteins related to C. carandas L. were selected from super pred databases and constructed Compound–targets–pathways network. General observation determination of cardiac markers enzymes, heart tissue homogenate analysis, body weight assessment, electrocardiogram (ECG) pattern analysis, and histopathological studies were conducted. Network pharmacology analysis identified the PI3K-Akt signaling pathway as a key pathway modulated by the compound, with a false discovery rate of 2.78E-05 and a strength of 1.21. A protein-pathway compound interaction network was established, highlighting MAPK1, NOS3, EP300, and STAT3 as having the highest edge counts at 14, 11, 9, and 9. CP administration significantly decreased body weight and increased heart weight. CP elevated levels of biomarker enzymes like creatine kinase isoenzyme MB, aspartate transaminase, alanine transaminase, and alkaline phosphatase. Furthermore, CP-induced rats showed significant deviations in these parameters compared to the normal group (heart rate: 264.83 ± 3.06 beats per minute; P-R interval: 0.064 ± 0.0001 msec; Q-T interval: 0.068 ± 0.0003 msec; R wave amplitude: 0.24 ± 0.004 mV), indicative of cardiotoxicity and significantly reduced superoxide dismutase (SOD) activity, elevated cardiac malondialdehyde levels and decreased glutathione levels. The histopathological examination provided evidence indicating a potential cardioprotective effect, as it revealed a decrease in the severity of cellular damage within the myocardium. These findings suggest the potential therapeutic utility of C. carandas L in managing chemotherapy-induced cardiotoxicity, highlighting its significance in improving the quality of life and treatment outcomes for cancer patients.
Formulation and Characterization of Chrysin Loaded Phytosomes and its Cytotoxic Effect against Colorectal Cancer Cells Namit Kudatarkar, Sunil Jalalpure, Bhaskar Kurangi Indian Journal of Pharmaceutical Education and Research, 2022 Background: Chrysin is a phytoconstituent which has anticancer activity. The study aims to formulate, characterize and evaluate the cytotoxic effect of chrysin loaded phytosomes against HT29 cells. Materials and Methods: Antisolvent precipitation technique was employed to prepare phytosomes. Particle size, polydispersity index, zeta potential, entrapment efficiency, scanning electron microscope and Fourier transform infrared spectroscopic analysis were carried out for the characterization of chrysin loaded phytosomes. Cell viability was done to evaluate the cytotoxic effect of developed phytosomes comparing with plain chrysin. Results: The developed chrysin loaded phytosomes showed the particle size of 94.40nm, polydispersity index of 0.31, and zeta potential -1.33 mV. The entrapment efficiency was 74.28 %. Chrysin loaded phytosomes showed increased cytotoxic effect on HT-29 cells. Conclusion: This research work produces confirmative indication for the use of Chrysin loaded phytosomes in experimental animals to further gain in depth analysis for anticancer activity of chrysin loaded phytosomes against colon cancer. ABSTRACT Mr. is His areas of research include screening of phytoconstituents and drugs for cancer using in vitro and in vivo studies. Prof. (Dr.) Sunil Jalalpure is presently working as a Professor and Principal, KLE College of KLE Academy of Education and Research, Belagavi. His areas of research include isolation/ characterization of active principles from medicinal plants and their pharmacological screening for various biological activities and training the research students in Pharmacognosy, Phytochemistry and Biotechnological aspects with modern tools and techniques. He is actively involved in nanoparticles drug delivery system of herbal phytoconstituents and green nanotechnology.
System biology and chemoinformatics approaches to decode the molecular mechanisms of Chrysin against colon cancer Kudatarkar Namit, Jalalpure Sunil, Patil Vishal S., Kurangi Bhaskar Journal of Applied Pharmaceutical Science, 2021 Namit Kudatarkar1† , Sunil Jalalpure2* , Vishal S. Patil1,3† , Bhaskar Kurangi4 1Department of Pharmacology, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, India. 2Department of Pharmacognosy, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, India. 3ICMR-National Institute of Traditional Medicine, Belagavi, India. 4Department of Pharmaceutics, KLE College of Pharmacy, Belagavi, KLE Academy of Higher Education and Research (KAHER), Belagavi, India.
Analytical method development and validation for estimation of chrysin in chrysin loaded phytosomes using high performance thin layer chromatography Namit Kudatarkar, Sunil Jalalpure, Amruta Balekundri, Bhaskar Kurangi Journal of Liquid Chromatography and Related Technologies, 2021 Chrysin is a potential flavonoid used in the treatment of various diseases and disorders, some of the major ones are inflammation, gout, HIV-AIDS, and cancer. In the present study, the Chrysin Phytosomes are prepared by applying the anti-solvent procedure and are quantified using an analytical method that is High Performance Thin Layer Chromatography (HP-TLC) which is developed and validated in accordance with International Council for Harmonization guidelines. In the method development for chrysin, silica gel aluminum plate 60 F254 is been used as stationary phase and the mobile phase used is n-hexane:ethyl acetate:methanol:formic acid in the ratio of 8:8:1:0.2 v/v/v/v is used. The method is validated for linearity, range, limit of detection, limit of quantification, precision, and robustness. The developed analytical method was found to be simple, reliable, precise, and robust. The validated High Performance Thin Layer Chromatography method was successfully applied for quantification of chrysin in chrysin-loaded phytosomes.
Pharmacological evaluation of caesalpinia pulcherrima leaf extract for anticancer activity against ehrlich ascites carcinoma bearing mice Shamanand Mallapur, Prakash Rajshekhar Biradar, Satish Annayya Kavatagimath, Priyanka Patil, Vishal Patil, Namit Kudatarkar Indian Journal of Pharmaceutical Education and Research, 2021 Objectives: The aim of the study is to assess the anticancer activity of Caesalpinia pulcherrima leaf extract against Erlich Ascites Carcinoma (EAC) in mice. Materials and Methods: BALB/c mice with Erlich Ascites Carcinoma were used for the present study. Ethanolic extract of C. pulcherrima (EECP) was administered p.o. in three different doses i.e. 200, 400 and 800 mg/kg body weight, after 24 hr of tumor inoculation in mice for 14 days. Effect of EECP on haematological parameters, liver enzymes marker, biochemical parameters/ antioxidants was evaluated after 14 days treatment. Results: Effect of EECP on Haematological parameters were evaluated and Hb, Lymphocytes, RBC and Monocytes showed significant increase in their count when compared to Disease. WBC and neutrophil count was decreased in EECP group compared to disease. Effect of EECP on Serum parameters were evaluated and showed significant decrease in ALP, TC, AST, ALT and TG compared to disease. TP was significantly increased in EECP groups compared to DISEASE group. Effect of EECP on Antioxidant activity showed significant increase in CAT, SOD, GSH and GPx counts compared to Disease. LPO level was significantly reduced in all the treated groups compared to Disease group. Conclusion: Ethanolic extract of C. pulcherrima leaf exhibited significant antioxidant and anticancer activity against Erlich Ascites Carcinoma.
