Olga Shydlovska

@knutd.edu.ua

DEPARTMENT OF BIOTECHNOLOGY, LEATHER AND FUR/Faculty of Chemical and Biopharmaceutical Technologies
Kyiv National University of Technologies and Design

Olga Shydlovska


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https://researchid.co/o.shydlovska

RESEARCH INTERESTS

biotechnology, virology, microbiology, genetics, molecular biology, immunology, agrotechnology, nanotechnology
9

Scopus Publications

113

Scholar Citations

7

Scholar h-index

5

Scholar i10-index

Scopus Publications

  • Metal-based nanoparticles: an alternative treatment for biofilm infection in hard-to-heal wounds
    Olga Iungin, Olga Shydlovska, Olena Moshynets, Volodymyr Vasylenko, Marina Sidorenko, et al.
    Journal of Wound Care, 2024
    Metal-based nanoparticles (MNPs) are promoted as effective compounds in the treatment of bacterial infections and as possible alternatives to antibiotics. These MNPs are known to affect a broad spectrum of microorganisms using a multitude of strategies, including the induction of reactive oxygen species and interaction with the inner structures of the bacterial cells. The aim of this review was to summarise the latest studies about the effect of metal-based nanoparticles on pathogenic bacterial biofilm formed in wounds, using the examples of Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Pseudomonas aeruginosa, as well as provide an overview of possible clinical applications.
  • Antibacterial Activity of Green Synthesised Silver Nanoparticles on Saccharomyces cerevisiae
    Yugin Kharchenko, Liudmyla Lastovetska, Valeriia Maslak, Marina Sidorenko, Volodymyr Vasylenko, et al.
    Applied Sciences Switzerland, 2022
    Green synthesis of nanoparticles is a widely researched and popular direction in the development of nanotechnology. It is a simple, cheap and effective method for obtaining nanoparticles with interesting biological properties. In light of the development of antibiotic resistance to important clinical strains of bacteria, this method was used in the present study to obtain silver nanoparticles with antibacterial activity. The aim of this study was to synthesise silver nanoparticles with antibacterial action by yeast in a process known as “green synthesis”. We are also considering the prospect of using silver nanoparticles as an antibacterial substance for drug development. The production of nanoparticles was confirmed by UV spectroscopy. Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922 test strains and Staphylococcus aureus 1536 and Klebsiella pneumoniae 520 clinical isolates were used to study the antibacterial effect. The effect of synthesised nanoparticles on the metabolic activity of bacterial cells and their ability to adhere, as well as the minimum inhibitory concentrations (MICs) of synthesised nanoparticles for each of the strains, were determined. Following UV spectroscopy, the nanoparticles obtained were found to have a pronounced peak in optical absorption at 400 nm, corresponding to the plasmon resonance of silver nanoparticles, and demonstrated a high antibacterial effect against all the strains studied.
  • REVIEW OF GREEN METHODS OF SYNTHESIS OF SILVER NANOPARTICLES
    Olga Shydlovska, Yevhen Kharchenko
    Icams Proceedings of the International Conference on Advanced Materials and Systems, 2022
    Green synthesis of metal nanoparticles is a very promising area of research. Silver nanoparticles are the most interesting type of nanoparticle in nanotechnology because they have varied properties, such as antibacterial, antioxidant, and antibiofilm forming properties. This review aims to establish which of the most common approaches for the biological synthesis of nanoparticles is the best. In this work, the methods of synthesis of silver nanoparticles using plant extracts, bacteria, and yeast are considered. Each of these methods has its advantages and disadvantages. The most common method of synthesis of silver nanoparticles is the method using plant extracts, however, stabilizing substances from plant extracts have their own direct biological activity, which can be both enhanced and suppressed by silver nanoparticles. Green synthesis of nanoparticles thanks to microorganisms makes it possible to use a wide range of bacterial strains, but it is important to remember of the pathogenicity of the strains and their danger to humans. From this perspective, the use of yeast for the synthesis of silver nanoparticles is the most promising method, as it allows obtaining a large amount of nanomaterial. The synthesis, thanks to yeast method, allows us to control the size and shape of the nanoparticles. Nanoparticles obtained from yeast lysates have effective antibacterial and antifilm-forming activity.
  • L. lactis BACTERIOPHAGES AND METHODS OF THEIR ELIMINATION FROM DAIRY PRODUCTS
    Olga Shydlovska, Yuliia Khmelnytska
    Icams Proceedings of the International Conference on Advanced Materials and Systems, 2022
    Dairy products are important in human diet and nutrition. That is why dairy production is critical not only economically, but also socially and medically. In recent decades, dairy production has had problems with disturbances in fermentation processes caused by bacteriophage contamination. It is important to note that every year there are new reports about newly discovered bacteriophages that disrupt fermentation processes in the production of kefir, yogurt, and various types of cheese. Lactococcus lactis strains are of particular importance in dairy technology, as they are used for the production of various yogurts and cheeses. The study of the spectrum of bacteriophages infecting this strain can help to monitor the evolutionary changes of viruses and the horizontal transfer of genes. In this paper, an analysis of phages infecting L. lactis was carried out. Most bacteriophages belong to the Siphoviridae and Podoviridae families. Moreover, the authors analyzed approaches that can be used to reduce bacteriophage contamination in the production of dairy products. It has been shown that the use of disinfectants, such as ethanol on sodium hypochlorite, can reduce the titer of bacteriophages and protect products from the development of viral infection. It is also possible to use membrane filtration with UV irradiation. Moreover, all these approaches can be combined to achieve the most effective result.
  • In vitro cytotoxicity of cerium dioxide nanoparticles and its effect on nitrite-ion production in normal and transplantable cell lines
    Olga A. Shydlovska, Nadiya M. Zholobak
    Proceedings of the 2017 IEEE 7th International Conference on Nanomaterials Applications and Properties Nap 2017, 2017
    In the research, we were used normal cells that were received ex vivo, and transfected cell lines to establish the toxicity of non-stabilized and citrate-stabilized nanoparticles of cerium dioxide and to study their influence on the nitrite ion production. As a result, there was established the most effective concentrations of different type cerium dioxide nanoparticles and was showed the different response of normal and tumor cell lines.
  • Synthesis and comparative characteristics of biological activities of (La, Sr)MnO3 and Fe3O4 nanoparticles
    Olga Shydlovska, Nadiya Zholobak, Svitlana Dybkova, Sergej Osinsky, Larissa Bubnovskaya, et al.
    European Journal of Nanomedicine, 2017
    In the last decade, ferromagnetic nanoparticles that are able to be heated under an AMF (alternating magnetic field) have gained considerable interest in the field of nanotechnology. The current study explores the peculiarity of the synthesis and the properties of Fe
  • Influence of the virus-nanoparticles system illumination on the virus infectivity
    O. Khylko, N. Rusinchuk, O. Shydlovska, M. Lokshyn, V. Lozovski, et al.
    Journal of Bionanoscience, 2016
  • Cerium dioxide nanoparticles increase immunogenicity of the influenza vaccine
    Nadezhda M. Zholobak, Alla P. Mironenko, Alexander B. Shcherbakov, Olga A. Shydlovska, Mykola Ya. Spivak, et al.
    Antiviral Research, 2016
    We have demonstrated the influence of cerium dioxide nanoparticles on the immunogenicity of the influenza vaccine on an example of liquid split inactivated Vaxigrip vaccine. Antibody titers were analyzed using the hemagglutination inhibition (HI) assay. Seroprotection, seroconversion, the geometric mean titers (GMTs) and the factor increase (FI) in the GMTs were calculated. The effect of nano-ceria surface stabilizer on the enhancement of immunogenicity was shown. The vaccine modified by citrate-stabilized nano-ceria, in contrast to a non-modified Vaxigrip vaccine, did not provide an adequate level of seroprotection, and seroconversion after vaccination was 66.7% on days 49-63 for virus strain А(H1N1) and 100% on day 49 for virus strain B/Yamagata. For the low immunogenic influenza B virus, the rise in antibody titers (GMT/IF) was 24.38/3.28 after the first injection and 50.40/6.79 on day 49. For the vaccine modified by non-stabilized nano-ceria, for all virus strains under study, on day 63, upon immunization notable levels of seroprotection, seroconversion and GMT/IF were registered (higher than for the non-modified Vaxigrip vaccine). The successful attempt to modify the influenza vaccine demonstrates the possible ways of increasing the specific activity of vaccines using nano-ceria.
  • Purification of bioliquids from viruses by surface plasmon-polaritons
    M. Lokshyn, V. Lozovski, V. Lysenko, Yu. Ushenin, N. Rusinchuk, et al.
    Journal of Bionanoscience, 2015

RECENT SCHOLAR PUBLICATIONS

  • Metal-based nanoparticles: an alternative treatment for biofilm infection in hard-to-heal wounds
    O Iungin, O Shydlovska, O Moshynets, V Vasylenko, M Sidorenko, ...
    Journal of Wound Care 33 (Sup4a), xcix-cx , 2024
    2024
  • Research of the antibacterial effect of nano-filled polymer materials
    O Ishchenko, V Plavan, O Shydlovska, Y Kharchenko
    Technologies and Engineering 5 (24), 96-103 , 2023
    2023
  • THE PROSPECTS OF USING LACTOBACILLUS DELBRUECKII FOR APPLE JUICE FERMENTATION
    O Shydlovska, I Motrenko
    EUROPEAN DIMENSIONS OF THE SUSTAINABLE DEVELOPMENT and ACADEMIC–BUSINESS … , 2023
    2023
  • Obtaining a functional product based on fermented Lactobacillus acidophilus apple juice and studying its properties
    O Shydlovska, A Koiba
    Biological Systems: Theory and Innovation 1 (14), 13-25 , 2023
    2023
    Citations: 3
  • POTENTIAL USE OF S. CEREVISIAE YEAST FOR THE PRODUCTION OF GLUTEN-FREE BAKERY PRODUCTS
    AD Olga Shydlovska
    The XX International Scientific and Practical Conference «Ways of distance … , 2023
    2023
  • Отримання функціонального продукту на основі ферментованого Lactobacillus acidophilus яблучного соку та дослідження його властивостей
    O Shydlovska, А Koiba
    Biological Systems: Theory and Innovation 14 (1), 13-25 , 2023
    2023
  • ДОСЛІДЖЕННЯ АНТИБАКТЕРІАЛЬНОГО ЕФЕКТУ НАНОНАПОВНЕНИХ ПОЛІМЕРНИХ МАТЕРІАЛІВ
    О ISHCHENKO, V PLAVAN, O SHYDLOVSKA, Y KHARCHENKO
    Technologies and Engineering, 96-103 , 2023
    2023
  • Antibacterial Activity of Green Synthesised Silver Nanoparticles on Saccharomyces cerevisiae
    Y Kharchenko, L Lastovetska, V Maslak, M Sidorenko, V Vasylenko, ...
    Applied Sciences 12 (7), 3466 , 2022
    2022
    Citations: 9
  • Review of green methods of synthesis of silver nanoparticles
    O Shydlovska, Y Kharchenko
    ICAMS proceedings of the international conference on advanced materials and … , 2022
    2022
    Citations: 7
  • Наночастки діоксиду церію як універсальний ад’ювант для біологічно-активних молекул
    ЖНМ Шидловська О.А.
    “Актуальні проблеми мікробіології, вірусології та імунології”, 147 , 2019
    2019
  • ИСПОЛЬЗОВАНИЕ SACCHAROMYCES В ЖИВОТНОВОДСТВЕ
    БТА Волошина И.Н., Шидловская О.А.
    VІІІ Міжнародна науково-практична онлайн конференція студентів, аспірантів … , 2019
    2019
  • ПЕРСПЕКТИВИ ЗАСТОСУВАННЯ НАНОЧАСТОК ДІОКСИДУ ЦЕРІЮ В БІОТЕХНОЛОГІЇ РОСЛИН
    ШОА СКОРОХОД С.О., КОЛОСІНСЬКА Н. І.
    VI Всеукраїнська науково-практична конференція молодих учених і студентів … , 2019
    2019
  • ПЕРСПЕКТИВИ КОМБІНОВАНОГО ЗАСТОСУВАННЯ ПРЕПАРАТУ ТИЛОРОНУ ТА НАНОЧАСТОК ДІОКСИДУ ЦЕРІЮ
    ШОА КОСТЮЧЕНКО В.А., АППОЛОНОВА І. Є.
    VI Всеукраїнська науково-практична конференція молодих учених і студентів … , 2019
    2019
  • Ефективність застосування аміксину (тилорону дигідрохлориду) проти РНК- та ДНК-вмісних вірусів
    СМЯ Жолобак Н.М., Шидловська О.А., Щербань В.Л., Бабій О.В., Богорад ...
    Актуальна інфектологія 7 (5), 89 , 2019
    2019
  • B IO TEC HNO LO G IC AL ASPECTS OF WA STEW A TER TR EA TM ENT OF ENTERPRISES THAT PROCESS L IVE S TO CK PRODUCTS
    P Rebrykova, O Shydlovska, N Zholobak, O Mokrousova
    2018
  • Nanoparticles of cerium dioxide–an effective antiviral agent and adjuvant of biologically active molecules
    O Shydlovska, E Kharchenko, I Osenniy, M Spivak, A Shcherbakov, ...
    ScienceRise: Biological Science, 26-30 , 2018
    2018
    Citations: 10
  • Біотехнологічні аспекти очищення стічних вод підприємств, що переробляють продукти тваринництва
    ПА Ребрикова, ОА Шидловська, НМ Жолобак, ОР Мокроусова
    Наукові праці Національного університету харчових технологій, 42-49 , 2018
    2018
    Citations: 3
  • Cerium oxide nanoparticles increase the cytotoxicity of TNF-alpha in vitro
    O Shydlovska, E Kharchenko, N Zholobak, A Shcherbakov, A Marynin, ...
    Наносистемы: физика, химия, математика 9 (4), 537-543 , 2018
    2018
    Citations: 6
  • Наночастинки діоксиду церію–ефективний антивірусний засіб та ад’ювант біологічно-активних молекул
    ОА Шидловська, Є Харченко, ІМ Осінній, МЯ Співак, ОБ Щербаков, ...
    ScienceRise. Biological science, 26-30 , 2018
    2018
    Citations: 6
  • In vitro cytotoxicity of cerium dioxide nanoparticles and its effect on nitrite-ion production in normal and transplantable cell lines
    OA Shydlovska, NM Zholobak
    2017 IEEE 7th International Conference Nanomaterials: Application … , 2017
    2017

MOST CITED SCHOLAR PUBLICATIONS

  • Cerium dioxide nanoparticles increase immunogenicity of the influenza vaccine
    NM Zholobak, AP Mironenko, AB Shcherbakov, OA Shydlovska, ...
    Antiviral research 127, 1-9 , 2016
    2016
    Citations: 30
  • Synthesis and comparative characteristics of biological activities of (La, Sr) MnO3 and Fe3O4 nanoparticles
    O Shydlovska, N Zholobak, S Dybkova, S Osinsky, L Bubnovskaya, ...
    European Journal of Nanomedicine 9 (1), 33-43 , 2017
    2017
    Citations: 13
  • Purification of bioliquids from viruses by surface plasmon-polaritons
    M Lokshyn, V Lozovski, V Lysenko, Y Ushenin, N Rusinchuk, ...
    Journal of Bionanoscience 9 (6), 431-438 , 2015
    2015
    Citations: 13
  • Influence of the virus-nanoparticles system illumination on the virus infectivity
    N Zholobak
    Journal of Bionanoscience , 2016
    2016
    Citations: 11
  • Nanoparticles of cerium dioxide–an effective antiviral agent and adjuvant of biologically active molecules
    O Shydlovska, E Kharchenko, I Osenniy, M Spivak, A Shcherbakov, ...
    ScienceRise: Biological Science, 26-30 , 2018
    2018
    Citations: 10
  • Antibacterial Activity of Green Synthesised Silver Nanoparticles on Saccharomyces cerevisiae
    Y Kharchenko, L Lastovetska, V Maslak, M Sidorenko, V Vasylenko, ...
    Applied Sciences 12 (7), 3466 , 2022
    2022
    Citations: 9
  • Review of green methods of synthesis of silver nanoparticles
    O Shydlovska, Y Kharchenko
    ICAMS proceedings of the international conference on advanced materials and … , 2022
    2022
    Citations: 7
  • Cerium oxide nanoparticles increase the cytotoxicity of TNF-alpha in vitro
    O Shydlovska, E Kharchenko, N Zholobak, A Shcherbakov, A Marynin, ...
    Наносистемы: физика, химия, математика 9 (4), 537-543 , 2018
    2018
    Citations: 6
  • Наночастинки діоксиду церію–ефективний антивірусний засіб та ад’ювант біологічно-активних молекул
    ОА Шидловська, Є Харченко, ІМ Осінній, МЯ Співак, ОБ Щербаков, ...
    ScienceRise. Biological science, 26-30 , 2018
    2018
    Citations: 6
  • Obtaining a functional product based on fermented Lactobacillus acidophilus apple juice and studying its properties
    O Shydlovska, A Koiba
    Biological Systems: Theory and Innovation 1 (14), 13-25 , 2023
    2023
    Citations: 3
  • Біотехнологічні аспекти очищення стічних вод підприємств, що переробляють продукти тваринництва
    ПА Ребрикова, ОА Шидловська, НМ Жолобак, ОР Мокроусова
    Наукові праці Національного університету харчових технологій, 42-49 , 2018
    2018
    Citations: 3
  • Response of interferon system to introduction of recombinant interferon modified with cerium dioxide nanoparticles
    O Shydlovska, N Zholobak, A Shcherbakov, M Spivak, ...
    Proceedings of VIII International conference “Bioresources and viruses … , 2015
    2015
    Citations: 2
  • Metal-based nanoparticles: an alternative treatment for biofilm infection in hard-to-heal wounds
    O Iungin, O Shydlovska, O Moshynets, V Vasylenko, M Sidorenko, ...
    Journal of Wound Care 33 (Sup4a), xcix-cx , 2024
    2024
  • Research of the antibacterial effect of nano-filled polymer materials
    O Ishchenko, V Plavan, O Shydlovska, Y Kharchenko
    Technologies and Engineering 5 (24), 96-103 , 2023
    2023
  • THE PROSPECTS OF USING LACTOBACILLUS DELBRUECKII FOR APPLE JUICE FERMENTATION
    O Shydlovska, I Motrenko
    EUROPEAN DIMENSIONS OF THE SUSTAINABLE DEVELOPMENT and ACADEMIC–BUSINESS … , 2023
    2023
  • POTENTIAL USE OF S. CEREVISIAE YEAST FOR THE PRODUCTION OF GLUTEN-FREE BAKERY PRODUCTS
    AD Olga Shydlovska
    The XX International Scientific and Practical Conference «Ways of distance … , 2023
    2023
  • Отримання функціонального продукту на основі ферментованого Lactobacillus acidophilus яблучного соку та дослідження його властивостей
    O Shydlovska, А Koiba
    Biological Systems: Theory and Innovation 14 (1), 13-25 , 2023
    2023
  • ДОСЛІДЖЕННЯ АНТИБАКТЕРІАЛЬНОГО ЕФЕКТУ НАНОНАПОВНЕНИХ ПОЛІМЕРНИХ МАТЕРІАЛІВ
    О ISHCHENKO, V PLAVAN, O SHYDLOVSKA, Y KHARCHENKO
    Technologies and Engineering, 96-103 , 2023
    2023
  • Наночастки діоксиду церію як універсальний ад’ювант для біологічно-активних молекул
    ЖНМ Шидловська О.А.
    “Актуальні проблеми мікробіології, вірусології та імунології”, 147 , 2019
    2019
  • ИСПОЛЬЗОВАНИЕ SACCHAROMYCES В ЖИВОТНОВОДСТВЕ
    БТА Волошина И.Н., Шидловская О.А.
    VІІІ Міжнародна науково-практична онлайн конференція студентів, аспірантів … , 2019
    2019