Effects of 1,25-Dihydroxycholecalciferol Glycoside Supplementation on the Growth, Intestinal Health, and Immunity of Broilers from Breeders Supplemented or Not with the Same Additive Thiago S. Andrade, Nilton Rohloff Junior, Paulo L. O. Carvalho, Bruno S. Vieira, José G. Vargas Junior, et al. Veterinary Sciences, 2025 This study aimed to investigate the effects of 1,25-Dihydroxycholecalciferol glycoside (1,25(OH)2D3-G) supplementation on the performance, biochemical blood, bone health, intestinal histomorphometry, and gene expressions in broilers from breeders supplemented or not with 1,25(OH)2D3-G. A total of 1152 one-day-old, male Ross 308 AP chicks were distributed in a completely randomized design with a 2 × 3 factorial arrangement. One factor was the inclusion or not of 1,25(OH)2D3-G in the breeders’ diets from 21 to 62 weeks of age. The other factor involved three levels (0, 50, and 100 mg/kg) of 1,25(OH)2D3-G inclusion in the broilers’ diets from 1 to 21 days of age. The study totaled six treatments, with eight replicates and 24 birds per experimental unit. Breeders supplemented with of 1,25(OH)2D3-G resulted in heavier broiler chicks at hatch, better feed conversion, and higher concentrations of calbindin D28K, interleukin 10, and interleukin 1β at 21 days of age. Villus height and absorption area in the jejunum showed interactions between breeder supplementation and broiler diets. The inclusion of this additive in both diets was not sufficient to increase villus height and absorption area in broilers at 21 days of age. It is concluded that supplementation in breeders improves growth and immunity in broilers.
Efficacy of organic acids in feed experimentally contaminated with Aspergillus flavus Rogerio Aleson Dias Bezerra, Maria Eduarda Ribeiro Ratão, Thainá Silva Blasques, Bruna Barnei Saraiva, Gabriel Amaral de Araújo, et al. Ciencia Animal Brasileira, 2025 Organic acids have been shown to be a promising alternative to chemical compounds due to their ability to inhibit microbial growth. The aim of this study was to evaluate the antifungal activity of organic acids in animal feed experimentally contaminated with Aspergillus flavus. The A. flavus strain (ACFV) was activated in the Sabouraud Dextrose Agar way and incubated at 25 °C for 7 days. After incubation, it was carried out the preparation of the spore suspensions and counted in a Neubauer chamber, standardizing the inoculum at 5.0 x 10-6 spores/mL. Spore suspensions (10 mm agar discs) were prepared in buffered peptone water (1000 mL) and the feeds were treated with commercial organic acids, including propionic acid or a blend of acids (propionic, tartaric, citric, formic, sorbic and lactic). The feed was analyzed on days 1, 3, 5 and 7 of shelf life, with microbiological analysis of mold and Aspergillus spp. count, in addition to measurements of water activity, dry matter, pH and temperature. Organic acids reduced the total count of filamentous fungi throughout the evaluation period. A significant reduction in Aspergillus spp. counts was observed in feeds treated with organic acids (p<0.05). Furthermore, there was a reduction in dry matter and pH in the feeds. It is concluded that organic acids have great potential to inhibit fungal growth in diets, ensuring their quality and safety during the storage.
Oral tryptophan supplementation to suckling piglets affecting hypothalamic serotonin metabolism, behavior, and growth in the nursery period under social mixing stress Tiago J Pasquetti, Alexa R Gormley, Jiyao Guo, Paulo C Pozza, Sung Woo Kim Journal of Animal Science, 2025 Tryptophan (Trp) is the rate-limiting substrate for the synthesis of serotonin (5-hydroxytryptamine; 5-HT) in the brain, which plays a central role in the regulation of stress sensitivity and feed intake. The objective of this study was to evaluate the effect of oral Trp supplementation to suckling piglets on 5-HT metabolism, growth performance, and behavior during the suckling (14 to 21 d of age) and nursery (21 to 56 d of age) periods, under social mixing stress at weaning. Eighty 14-d-old suckling piglets from 10 sows (8 piglets per litter) were allotted to 2 treatments. The 2 treatments (0.389 g alanine + 0.430 g glucose/kg body weight (BW)/d or 0.467 g Trp/kg BW/d) were given orally to piglets 7 times daily, beginning at 0700 h in 2 h intervals, from day 14 to day 21 of age (suckling period). Alanine (Ala) was used to balance the nitrogen content when Trp was added. Growth performance was measured from day 14 to day 21 of age (suckling period) and from day 21 to day 56 of age (nursery period) in 2 phases. On day 21, 12 piglets from 12 L (6 pigs/treatment) were selected and euthanized to collect hypothalamus to measure 5-HT and 5-hydroxyindoleacetic acid (5-HIAA) concentrations. The remaining piglets were weaned and moved to nursery pens. Pigs within the same treatment and sex, but from different litters, were paired and housed together (2 pigs/pen). All pigs were fed the same diet during the nursery period. Normal and aggressive behaviors were recorded for 24 h immediately after weaning. Oral Trp supplementation during the suckling period increased (P &lt; 0.05) hypothalamic concentration of 5-HT and 5-HIAA at weaning, whereas growth performance was not affected. However, oral Trp supplementation tended to increase (P = 0.057) the frequency of visits to the feeders during social mixing stress. In conclusion, oral Trp supplementation during the suckling period increased hypothalamic serotonin and could influence eating behavior postweaning.
Ratio of Linoleic and Alpha-Linolenic Acids and Vitamin E: The Productive Performance, Serum Biochemical Profile, and Yolk Composition of Japanese Quail Breeders LAL Silva, BC Rosário Silva, MT Frageri Paulino, FE De Marchi, PC Pozza, et al. Revista Brasileira De Ciencia Avicola Brazilian Journal of Poultry Science, 2025 Vitamin E in poultry has beneficial effects on productive and reproductive performance. It also acts as an antioxidant, protecting polyunsaturated fatty acids from the omega-3 group, such as alpha-linolenic acid (ALA) and linoleic acid (LA). This paper aims to assess the effects of vitamin E (vitE) in diets containing two LA/ALA ratios on the productive performance, egg quality, egg composition, and biochemical parameters of Japanese quail breeders. A completely randomized design was used in a 2×3 factorial design with two LA/ALA ratios (9.29:1 and 13.75:1) × three vitamin E levels (25, 200, and 250 mg) with 10 replications of 6 females + 2 males. Data was submitted for analysis of variance in a factorial scheme using the Tukey test. There was no significant effect on performance and egg quality with the interactions. Eggs from females fed with an LA/ALA ratio of 9.29 had the lowest percentage of yolk, the highest percentage of albumen, and the highest luminosity. Females and males fed with the 13.75 ratio had higher levels of triglycerides and serum cholesterol. An interaction effect was observed for serum HDL and LDL levels in females. Breeders fed the diet with the 9.29 ratio and 200 mg of vitamin E has increased HDL levels and reduced LDL levels. The treatments had no effect on the bromatological composition of the yolk. In conclusion, the LA/ALA ratio of 9.29 with 200 mg of vitamin E supplementation can be fed to Japanese quail breeders without affecting productive performance. This ratio also can improve the biochemical composition of blood and yolk.
Efficacy of organic acids in feed experimentally contaminated with Aspergillus flavus Rogerio Aleson Dias Bezerra, Maria Eduarda Ribeiro Ratão, Thainá Silva Blasques, Bruna Barnei Saraiva, Gabriel Amaral de Araújo, et al. Ciencia Animal Brasileira, 2025 Resumo Os ácidos orgânicos têm se mostrado uma alternativa promissora aos compostos químicos devido à sua capacidade de inibir o crescimento microbiano. O objetivo deste estudo foi avaliar a atividade antifúngica de ácidos orgânicos em rações animais experimentalmente contaminadas com Aspergillus flavus. A cepa de A. flavus (ACFV) foi ativada em meio Ágar Sabouraud Dextrose e incubada a 25 °C por 7 dias. Após a incubação, foi realizada a preparação de suspensões de esporos e a contagem em câmara de Neubauer, padronizando o inóculo em 5,0 x 10-6 esporos/mL. Foram preparadas suspensões de esporos (discos de ágar 10 mm) em água peptonada tamponada (1000 mL) e as rações foram tratadas com ácidos orgânicos comerciais, incluindo ácido propiônico ou um blend de ácidos (propiônico, tartárico, cítrico, fórmico, sórbico e lático). A ração foi analisada nos dias 1, 3, 5 e 7 de vida de prateleira, com análise microbiológica de bolores e contagem de Aspergillus spp., além de medidas de atividade de água, matéria seca, pH e temperatura. Os ácidos orgânicos reduziram a contagem total de fungos filamentosos ao longo do período de avaliação. Observou-se uma redução significativa nas contagens de Aspergillus spp. nas rações tratadas com ácidos orgânicos (p<0,05). Além disso, houve redução da matéria seca e do pH nas rações. Conclui-se que os ácidos orgânicos têm grande potencial para inibir o crescimento fúngico em rações, assegurando sua qualidade e segurança durante o armazenamento.
Interaction effects of glycine equivalent and standardized ileal digestible threonine in low protein diets for broiler grower chickens Paschal Chukwudi Aguihe, Amanda Barroso Castelani, Camilo Ivan Ospina-Rojas, Eustace Ayemere Iyayi, Paulo Cesar Pozza, et al. Animal Bioscience, 2024 Objective: This study aims to investigate the interactive effect of a glycine equivalent (Glyequi) and standardized ileal digestible threonine (SID Thr) levels in low crude protein diets on performance, blood biochemistry, pectoral muscular creatine content and oxidative stability of meat in broiler chickens from 21 to 42 days.Methods: A total of 1,500, twenty-one-day-old Cobb-Vantress male broiler chickens were distributed in a completely randomized 5×3 factorial arrangement of Glyequi×SID Thr with five replicates of 20 birds each. Fifteen dietary treatments of 16.5% CP were formulated to contain five levels of total Glyequi (1.16%, 1.26%, 1.36%, 1.46%, and 1.56%) and three levels of SID Thr (0.58%; 0.68% and 0.78%).Results: Interaction effects (p<0.05) of Glyequi and SID Thr levels were observed for weight gain, carcass yield, pectoral muscular creatine content and serum uric acid. Higher levels of Glyequi increased (p = 0.040) weight gain in 0.58% and 0.68% SID Thr diets compare to the 0.78% SID Thr diet. The SID Thr level at 0.68% improved (p = 0.040) feed conversion compared to other SID Thr diets. Levels of Glyequi equal to or above 1.26% in diets with 0.78% SID Thr resulted in birds with higher (p = 0.033) pectoral muscular creatine content. The breast meat yield observed in the 0.68% SID Thr diet was higher (p = 0.05) compared to the 0.58% SID Thr diet. There was a quadratic effect of Glyequi levels for pectoral pectoral muscular creatine content (p = 0.008), breast meat yield (p = 0.030), and serum total protein concentrations (p = 0.040), and the optimal levels were estimated to be 1.47%, 1.35%, and 1.40% Glyequi, respectively. The lowest (p = 0.050) concentration of malondialdehyde in the breast meat was found in 0.68% SID Thr diets at 1.36% Glyequi. Conclusion: The minimum dietary level of Glyequi needed to improve performance in low crude protein diets is 1.26% with adequate SID Thr levels for broiler chickens.
Lycopene affects the immune responses of finishing pigs Marcelise Regina Fachinello, Nelson Luis Mello Fernandes, Eliezer Rodrigues de Souto, Tatiana Carlesso dos Santos, Alcides Emanuel Rodrigues da Costa, et al. Italian Journal of Animal Science, 2018
Potential probiotic lactobacilli of pig origin Mayara Andressa Sabedot, Josiane Mangoni, Magali Soares dos Santos Pozza, Paulo Cesar Pozza, Suzana De Almeida, et al. Acta Scientiarum Animal Sciences, 2011
Evaluation of inulin and probiotic for broiler chickens Wagner Thiago Mozer da Silva, Ricardo Vianna Nunes, Paulo Cesar Pozza, Magali Soares dos Santos Pozza, Matias Djalma Appelt, et al. Acta Scientiarum Animal Sciences, 2011
Energy values of the soybean meal for laying hens in function of the levels of inclusion and of crude protein in the reference diet Archives of Veterinary Science, 2007
Energy values of animal by-products for poultry Ricardo Vianna Nunes, Paulo César Pozza, Christiane Garcia Vilela Nunes, Evandro Campestrini, Rodrigo Kühl, et al. Revista Brasileira De Zootecnia, 2005
