Nanomedicine perspective in HIV therapy: biosynthesized gold nanoparticles exhibiting enhanced anti-HIV property M. R. Kamala Priya, Manickam Ashokkumar, Lucia K. Precilla, Luke Elizabeth Hanna, Priya R. Iyer Beni Suef University Journal of Basic and Applied Sciences, 2021 Background Globally, HIV/AIDS is a major public health issue. Currently, available antiretroviral therapy (ART) has a number of impediments. The need for long-term usage of antiretroviral drugs is associated with the problem of drug resistance, high cost, and other side effects. Hence, there is a constant urge for the development of novel drugs to combat the disease. The synthesis of nanoparticles is of great interest because of their reported application in diverse fields. In contrast to chemical synthesis, green synthesis is inexpensive and enviro-safe. Results Calophyllum inophyllum (CI) is a plant that is known to possess anti-HIV activity. Hence, this plant was exploited for the synthesis of gold nanoparticles (AuNPs) in the present study. Two parts of the plant, namely, the fruit and leaves (CIF and CIL) were used for the synthesis of nanocompounds. The two nanocompounds were tested on HIV-1 isolates at different concentrations and the EC50 values were determined. While CIF-AuNP demonstrated very good anti-HIV activity with an EC50 value of 0.09097 ng/ml, CIL-AuNPs did not show significant anti-HIV activity. Conclusions The present study is a novel attempt to produce nanocompounds that incorporate the medicinal properties of certain plants that are known to have anti-HIV activity within nanoparticles, such that the compound possesses the attributes of a nanomaterial alongside the phytoactivity. Our results provide evidence to suggest that the CIF-AuNP can be further explored for in vivo activity. In vitro cytotoxicity of the AuNPs was checked in VERO cell lines using the MTT assay. Cytotoxicity was observed to be minimal at all the tested concentrations.
Production Of Plastic Degrading Enzymes From Organisms Isolated From Plastic Polluted Areas Indian Journal of Environmental Protection, 2021
Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line Kamala Priya M R, Priya R. Iyer Egyptian Liver Journal, 2020 Background Currently, nanomaterials (NMs) research and development is at a fastest pace due to the enhanced implication in different areas of applications. The synthesis of such NMs through biosynthesis methods is gaining much importance because of low cost involved and environment-friendly approach. In this present study, the nanoparticles (NPs) are synthesized using a medicinal plant, with anticancer properties so as to incorporate the therapeutic activity within the NPs, such that the NPs will have the attributes of NMs alongside the phytoactivity. Results The synthesis of gold nanoparticles (AuNPs) using Graviola, the fruit of Annona muricata (Ramaphal fruit) commonly referred as Mullu seetha fruit (Tamil), was successfully carried out. The initial confirmations of the NPs were using UV-vis spectra, which showed the characteristic peak for the NPs. HRSEM analysis gave an insight on the size and morphology of the NPs. The zeta potential was measured to check the stability of the NPs. The cytotoxicity was carried out in VERO cell line and anticancer study in Hep2 liver cancer cell line. The surface plasmon resonance (SPR) band showed the characteristic peak at 536 nm for the AuNPs. In SEM micrographs, the size was ranging between 20 and 30 nm, on an average of 15 nm with spherical morphology. On the various tested concentrations in VERO cell line, the nanoparticles were non-toxic to the cells. The anticancer study gave an IC50 value at 10.94 μg/ml. Conclusions The NPs showed anticancer activity in treated Hep2 liver cancer cell line and as well as commendable non-toxic effect on normal VERO cell line. The results pose a positive impact to expand further studies in the development of potential drug molecules to tackle the disease of interest.
Apoptotic Activity in Cervical Cancer HeLa Cell Lines Treated with Chitosan Nanoconjugated Drug Doxorubicin – as Nanocarrier for Drug Delivery M. R. Kamala Priya, Priya R. Iyer Springer Proceedings in Materials, 2019 Nanocarriers play an indispensible role in drug delivery mechanisms thereby increasing the efficiency and overall activity of the specified drug in the given dosage. Chitosan is a well known biocompatible molecule that is exploited for various in vivo applications. In this current study, a biocompatible nanocarrier is developed as a conjugate using chitosan nanoparticles for the drug Doxorubicin. A nanoparticle is synthesized using the green extract of a medicinal plant. Upon successful synthesis, a chitosan conjugate is prepared along with the drug and synthesized nanoparticles. The synthesis of gold nanomaterials using Catharanthus roseus (Nithyakalyani) is successfully carried out. The initial confirmation of the nanoconjugate is from UV visible spectrophotometric analysis, which showed the characteristic peak for nanoparticles. SEM & HRSEM analysis revealed the size and shape of the nanocojugate. The Zeta potential is measured to check the stability of the nanoconjugate. The in vitro cytotoxicity is carried out in VERO cell lines and anti cancer study in cervical cancer HeLa cell lines. Further, the gene expression of one of the apoptotic molecule, Caspase was screened. The nanocarrier showed anti cancer activity in treated cervical cancer HeLa cell lines and as well as commendable non toxicity in treated normal vero cell lines. On this positive note, this nanocarrier study could be further expanded.
Isolation and characterization of pigments from microorganisms isolated from marine soil P. Senthamil Selvi, Priya Iyer Research Journal of Pharmacy and Technology, 2018 Bacterial pigments have many applications in current day to day life. The pigments produced by chromobacteria can be used for applications in dairy, pharmaceutical, food etc. In this study, 3 pigments were isolated i.e. Yellow from Xanthomonas sp., Pinkish Red from Rhodotorula sp., Orange from Sarcina sp. pigmented bacterial isolates obtained from the soil, were used for pigment extraction study. To isolate and characterize the Pigment producing bacteria from marine soil. We studied that the pigment producing bacteria and to identify the colour producing pigments. Soil samples from Pondicherry, Cuddalore, Chennai, and Andhra sea coast were collected and used for isolation of microbes producing pigments. Purification of extracted pigments done by column chromatography, whereas identification and characterization of purified pigment done by UV-Visible spectrophotometry and GC/MS analysis etc. The pigment isolated from bacteria was used for the antimicrobial activity, antioxidant, and anticancer and transformation studies. The bacterial extracts of carotenoid pigment extracted and used as natural colorants for food products and for dying of cloth.
Isolation, identification and antimicrobial activity of bioactive compound from saraca asoca International Journal of Current Pharmaceutical Review and Research, 2016
Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines M. R. Kamala Priya, Priya R. Iyer Applied Nanoscience Switzerland, 2015 It has been previously stated that gold nanoparticles have been successfully synthesized using various green extracts of plants. The synthesized gold nanoparticles were characterized under scanning electron microscopy and EDX to identify the size of the nanoparticles. It was found that the nanoparticles were around 30 nm in size, which is a commendable nano dimension achieved through a plant mediated synthesis. The nanoparticles were further studied for their various applications. In the current study, we have made attempts to exploit the anticancer ability of the gold nano particles. The nanoparticles were studied against MCF 7 breast cancer cell lines. The results obtained from the studies of anticancer activity showed that gold nanoparticles gave an equivalent good results, in par with the standard drugs against cancer. The AuNP’s proved to be efficient even from the minimum concentrations of 2 μg/ml, and as the concentration increased the anticancer efficacy as well increased.
Antibiotic efficacy of cinnamon (cinnamon zeylanicum) against urinary pathogens in type II diabetic men T. Sivapriya, Sheila John, Priya R. Iyer Journal of Herbs Spices and Medicinal Plants, 2014 The antibiotic efficacy of cinnamon (Cinnamon zeylanicum) powder on type-2 male diabetic subjects was investigated. A total of 24 type-2 male diabetic subjects were randomly assigned into experimental and control group. The experimental group consumed 1 g cinnamon powder capsule daily for a period of 45 d. Antimicrobial activity of cinnamon powder was evaluated against pathogens causing urinary tract infection before and after supplementation and compared against standard antibiotics. Urine culture examination indicated Escherichia coli as the most predominant pathogen causing urinary tract infection. The minimum inhibitory concentration (MIC) value of cinnamon extract was found to be 0.452 μg whereas the MIC of erythromycin and tetracycline were 10 μg and 30 μg, respectively. The supplementation of cinnamon capsule did not have any effect on gram-negative bacteria.
Comparative study of the production of biosurfactant by Pseudomonas fluorescens 2421 and Pseudomonas aeroginosa isolated from soil Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2013
Isolation, characterization and purification of chitinase from Penicillium, Pseudomonas and Bacillus amyloliquefaciens Asian Journal of Microbiology Biotechnology and Environmental Sciences, 2012
Antimicrobial activity of Aloevera barbedensis, Daucus carota, Emblica officinalis, Honey and Punica granatum and formulation of a health drink and salad Malaysian Journal of Microbiology, 2012
Deinking of note book papers and effuent degradation using Aspergillus fumigatus Iioab Journal, 2011
Antagonistic activity of probiotic organism against Vibrio cholerae and Cryptococcus neoformans Malaysian Journal of Microbiology, 2010
Production, purification and application of collagenase Journal of Pure and Applied Microbiology, 2008
RECENT SCHOLAR PUBLICATIONS
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In vitro efficacy of cinnamon derived copper nanoconjugates against drug-resistant and sensitive strains of Mycobacterium tuberculosis P Rajan, A Devi, A Dusthackeer, PR Iyer SCIREA Journal of Information Science and Systems Science 8 (4), 139-153 , 2024 2024
Determination of anti-tuberculosis activity of biosynthesized gold nanocompounds against M. tuberculosis H37RV MRK Priya, M Balasubramanian, CR Nirmal, A Dusthakeer, PR Iyer Indian Journal of Tuberculosis 70 (3), 329-338 , 2023 2023 Citations: 5
Isolation of bacteria producing lipase from copra (Cocos nucifera l.) Cake for degradation of vegetable waste oils KM Bethapudi, PR Iyer 2023
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A study on ER stress-induced apoptosis pathway in cervical cancer HeLa cells treated with biosynthesized gold nanoparticles MR Kamala Priya, PR Iyer Bulletin of the National Research Centre 45 (1), 212 , 2021 2021 Citations: 2
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Nanomedicine perspective in HIV therapy: biosynthesized gold nanoparticles exhibiting enhanced anti-HIV property MRK Priya, M Ashokkumar, LK Precilla, LE Hanna, PR Iyer Beni-Suef University Journal of Basic and Applied Sciences 10 (1), 23 , 2021 2021 Citations: 12
A review on antifungal agents against Candida albicans and Aspergillus niger R Vishnuvardhini, PR Iyer International Journal of Biological and Pharmaceutical Sciences Archive 2 … , 2021 2021 Citations: 5
Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line K Priya MR, PR Iyer Egyptian Liver Journal 10 (1), 15 , 2020 2020 Citations: 72
Characterisation of the biosynthesised gold nanomaterials-spectroscopic, dimensional, morphological, stability and structural analysis MRK Priya, PR Iyer International Journal of Biomedical Nanoscience and Nanotechnology 4 (3-4 … , 2020 2020 Citations: 3
Molecular and Phenotypic Evaluation of the Mesenchymal-Type Cell Population from Early-Phase Patient-Derived Cultures of Neuroblastoma for Personalized Drug Response Testing EXY Hee, MK Wong, SH Tan, ZX Chen, Z Choo, CH Kuick, SAC Ling, ... PEDIATRIC BLOOD & CANCER 66, S55-S55 , 2019 2019
Apoptotic Activity in Cervical Cancer HeLa Cell Lines Treated with Chitosan Nanoconjugated Drug Doxorubicin–as Nanocarrier for Drug Delivery MR Kamala Priya, PR Iyer International Conference on Nanomedicine, 42-53 , 2019 2019 Citations: 2
Chitosan based films incorporated with turmeric/clove/ginger essential oil for food packaging M Yashaswini, P Iyer J. Nanomed. Nanotechnol 10, 537 , 2019 2019 Citations: 17
Scalable Production of Collagen Isolated from Streptococcus pyogenes RM Akshaya, PR Iyer Int. J. Curr. Microbiol. App. Sci 8 (9), 2565-2578 , 2019 2019
Isolation and characterization of pigments from microorganisms isolated from marine soil PS Selvi, P Iyer Research Journal of Pharmacy and Technology 11 (10), 4296-4302 , 2018 2018 Citations: 4
Isolation and characterization of pigments from marine soil microorganisms PS Selvi, P Iyer IJLSSR 4, 2003-2011 , 2018 2018 Citations: 4
Biosciences and Plant Biology R Gautami, P Iyer Int. J. Curr. Res. Biosci. Plant Biol 5 (1), 60-66 , 2018 2018
Ex-traction of dopamine, serotonin, and gamma-aminobutyric acid from Lactobacillus spp and yeast A Pragalya, P Iyer International Journal of Neuroscience Research 2, 8 , 2018 2018 Citations: 3
Naringin loaded chitosan nanoparticle for bone regeneration: a preliminary in vitro study S Malathy, PR Iyer J. Nanomed. Nanotechnol 9 (4.10), 4172 , 2018 2018 Citations: 28
MOST CITED SCHOLAR PUBLICATIONS
An 80-tile 1.28 TFLOPS network-on-chip in 65nm CMOS S Vangal, J Howard, G Ruhl, S Dighe, H Wilson, J Tschanz, D Finan, ... 2007 IEEE International Solid-State Circuits Conference. Digest of Technical … , 2007 2007 Citations: 936
Antiproliferative effects on tumor cells of the synthesized gold nanoparticles against Hep2 liver cancer cell line K Priya MR, PR Iyer Egyptian Liver Journal 10 (1), 15 , 2020 2020 Citations: 72
Anticancer studies of the synthesized gold nanoparticles against MCF 7 breast cancer cell lines MR Kamala Priya, PR Iyer Applied nanoscience 5 (4), 443-448 , 2015 2015 Citations: 65
Antimicrobial efficacy of mango peel powder and formulation of recipes using mango peel powder (Mangifera indica L.) PA Thambi, S John, E Lydia, P Iyer, SJ Monica Int. J. Home Sci 2 (2), 155-161 , 2016 2016 Citations: 50
Antimicrobial activity of aloevera barbedensis, daucus carota, emblica officinalis, honey and punica granatum and formulation of a health drink and salad J Philip, S John, P Iyer Malays J Microbiol 8 (3), 141-147p , 2012 2012 Citations: 36
Naringin loaded chitosan nanoparticle for bone regeneration: a preliminary in vitro study S Malathy, PR Iyer J. Nanomed. Nanotechnol 9 (4.10), 4172 , 2018 2018 Citations: 28
Anti-microbial, anti-fungal and anti-carcinogenic properties of coconut milk kefir TS Lakshmi, A MaryPramela, P Iyer International Journal of Home Science 3 (1), 365-369 , 2017 2017 Citations: 23
Isolation and optimization of PHB (poly-β-hydroxybutyrate) based biodegradable plastics from Chlorella vulgaris R Robert, PR Iyer Journal of Bioremediation & Biodegradation 9 (2), 2-5 , 2018 2018 Citations: 20
Chitosan based films incorporated with turmeric/clove/ginger essential oil for food packaging M Yashaswini, P Iyer J. Nanomed. Nanotechnol 10, 537 , 2019 2019 Citations: 17
Testing high-speed IO links using on-die circuitry P Iyer, S Jain, B Casper, J Howard 19th International Conference on VLSI Design held jointly with 5th … , 2006 2006 Citations: 16
Nanomedicine perspective in HIV therapy: biosynthesized gold nanoparticles exhibiting enhanced anti-HIV property MRK Priya, M Ashokkumar, LK Precilla, LE Hanna, PR Iyer Beni-Suef University Journal of Basic and Applied Sciences 10 (1), 23 , 2021 2021 Citations: 12
Antibacterial activity of mangosteen (Garcinia mangostana) pericarp D Jacob, N Vigasini, PR Iyer World J. Pharm. Res 5 (9), 1797-1802 , 2016 2016 Citations: 11
Biosynthesis and optimization of highly stable gold nanoparticles, nanoconjugates, nanodrug conjugates and chitosan nanoconjugates using medicinal plants MRK Priya, PR Iyer Bulletin of the National Research Centre 46 (1), 134 , 2022 2022 Citations: 10
Isolation, identification and antimicrobial activity of bioactive compound from Saraca asoca M Maman, A Devi, P Iyer, ARJ Singh, P Jeyakumar Int J Curr Pharm Res 8 (1), 57-61 , 2016 2016 Citations: 9
Antimicrobial activity and characterisation of microflora of vinegar preparations developed from peels and fruit of sweet lime S Priyadarshini, S John, P Iyer European Journal of Biotechnology and Bioscience 2 (2), 42-45 , 2014 2014 Citations: 9
Isolation and identification of Bacillus thuringiensis and corroborate its insecticidal property S Jyothi, I Priya Journal Agricultural Science Food Research 9 (3), 4-6 , 2018 2018 Citations: 7
Extracellular rapid biosynthesis of AuNP’s using various green extracts of plants MR Kamala Priya, PR Iyer International journal of molecular biology & biochemistry 2, 33-40 , 2014 2014 Citations: 7
Isolation & characterization of carbofuran pesticide degrading microorganisms from various field areas in tamil nadu KY Devi, P Iyer Research Journal of Life Sciences, Bioinformatics, Pharmaceutical and … , 2017 2017 Citations: 6
Studies on the various parameters underlying the Synthesis of nanoparticles and the in-vitro stability of nanoparticles MR Kamala Priya, PR Iyer Front Biotechnol 1, 1-4 , 2014 2014 Citations: 6
Tuberculosis in buffaloes. PRK Iyer 1944 Citations: 6
