Priya Iyer
@wcc.edu.in
Associate Professor Biotechnology
Womens Christian College Chennai
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
M.R.Kamala Priya, Magizhaveni Balasubramanian, Christy Rosaline Nirmal, Azger Dusthakeer, and Priya R. Iyer
Elsevier BV
M. R. Kamala Priya, Manickam Ashokkumar, Lucia K. Precilla, Luke Elizabeth Hanna, and Priya R. Iyer
Springer Science and Business Media LLC
Abstract Background Globally, HIV/AIDS is a major public health issue. Currently, available antiretroviral therapy (ART) has a number of impediments. The need for long-term usage of antiretroviral drugs is associated with the problem of drug resistance, high cost, and other side effects. Hence, there is a constant urge for the development of novel drugs to combat the disease. The synthesis of nanoparticles is of great interest because of their reported application in diverse fields. In contrast to chemical synthesis, green synthesis is inexpensive and enviro-safe. Results Calophyllum inophyllum (CI) is a plant that is known to possess anti-HIV activity. Hence, this plant was exploited for the synthesis of gold nanoparticles (AuNPs) in the present study. Two parts of the plant, namely, the fruit and leaves (CIF and CIL) were used for the synthesis of nanocompounds. The two nanocompounds were tested on HIV-1 isolates at different concentrations and the EC50 values were determined. While CIF-AuNP demonstrated very good anti-HIV activity with an EC50 value of 0.09097 ng/ml, CIL-AuNPs did not show significant anti-HIV activity. Conclusions The present study is a novel attempt to produce nanocompounds that incorporate the medicinal properties of certain plants that are known to have anti-HIV activity within nanoparticles, such that the compound possesses the attributes of a nanomaterial alongside the phytoactivity. Our results provide evidence to suggest that the CIF-AuNP can be further explored for in vivo activity. In vitro cytotoxicity of the AuNPs was checked in VERO cell lines using the MTT assay. Cytotoxicity was observed to be minimal at all the tested concentrations.
Kamala Priya M R and Priya R. Iyer
Springer Science and Business Media LLC
Abstract Background Currently, nanomaterials (NMs) research and development is at a fastest pace due to the enhanced implication in different areas of applications. The synthesis of such NMs through biosynthesis methods is gaining much importance because of low cost involved and environment-friendly approach. In this present study, the nanoparticles (NPs) are synthesized using a medicinal plant, with anticancer properties so as to incorporate the therapeutic activity within the NPs, such that the NPs will have the attributes of NMs alongside the phytoactivity. Results The synthesis of gold nanoparticles (AuNPs) using Graviola, the fruit of Annona muricata (Ramaphal fruit) commonly referred as Mullu seetha fruit (Tamil), was successfully carried out. The initial confirmations of the NPs were using UV-vis spectra, which showed the characteristic peak for the NPs. HRSEM analysis gave an insight on the size and morphology of the NPs. The zeta potential was measured to check the stability of the NPs. The cytotoxicity was carried out in VERO cell line and anticancer study in Hep2 liver cancer cell line. The surface plasmon resonance (SPR) band showed the characteristic peak at 536 nm for the AuNPs. In SEM micrographs, the size was ranging between 20 and 30 nm, on an average of 15 nm with spherical morphology. On the various tested concentrations in VERO cell line, the nanoparticles were non-toxic to the cells. The anticancer study gave an IC50 value at 10.94 μg/ml. Conclusions The NPs showed anticancer activity in treated Hep2 liver cancer cell line and as well as commendable non-toxic effect on normal VERO cell line. The results pose a positive impact to expand further studies in the development of potential drug molecules to tackle the disease of interest.
M. R. Kamala Priya and Priya R. Iyer
Springer International Publishing
Nanocarriers play an indispensible role in drug delivery mechanisms thereby increasing the efficiency and overall activity of the specified drug in the given dosage. Chitosan is a well known biocompatible molecule that is exploited for various in vivo applications. In this current study, a biocompatible nanocarrier is developed as a conjugate using chitosan nanoparticles for the drug Doxorubicin. A nanoparticle is synthesized using the green extract of a medicinal plant. Upon successful synthesis, a chitosan conjugate is prepared along with the drug and synthesized nanoparticles. The synthesis of gold nanomaterials using Catharanthus roseus (Nithyakalyani) is successfully carried out. The initial confirmation of the nanoconjugate is from UV visible spectrophotometric analysis, which showed the characteristic peak for nanoparticles. SEM & HRSEM analysis revealed the size and shape of the nanocojugate. The Zeta potential is measured to check the stability of the nanoconjugate. The in vitro cytotoxicity is carried out in VERO cell lines and anti cancer study in cervical cancer HeLa cell lines. Further, the gene expression of one of the apoptotic molecule, Caspase was screened. The nanocarrier showed anti cancer activity in treated cervical cancer HeLa cell lines and as well as commendable non toxicity in treated normal vero cell lines. On this positive note, this nanocarrier study could be further expanded.
P. Senthamil Selvi and Priya Iyer
A and V Publications
M. R. Kamala Priya and Priya R. Iyer
Springer Science and Business Media LLC
It has been previously stated that gold nanoparticles have been successfully synthesized using various green extracts of plants. The synthesized gold nanoparticles were characterized under scanning electron microscopy and EDX to identify the size of the nanoparticles. It was found that the nanoparticles were around 30 nm in size, which is a commendable nano dimension achieved through a plant mediated synthesis. The nanoparticles were further studied for their various applications. In the current study, we have made attempts to exploit the anticancer ability of the gold nano particles. The nanoparticles were studied against MCF 7 breast cancer cell lines. The results obtained from the studies of anticancer activity showed that gold nanoparticles gave an equivalent good results, in par with the standard drugs against cancer. The AuNP’s proved to be efficient even from the minimum concentrations of 2 μg/ml, and as the concentration increased the anticancer efficacy as well increased.
T. Sivapriya, Sheila John, and Priya R. Iyer
Informa UK Limited
The antibiotic efficacy of cinnamon (Cinnamon zeylanicum) powder on type-2 male diabetic subjects was investigated. A total of 24 type-2 male diabetic subjects were randomly assigned into experimental and control group. The experimental group consumed 1 g cinnamon powder capsule daily for a period of 45 d. Antimicrobial activity of cinnamon powder was evaluated against pathogens causing urinary tract infection before and after supplementation and compared against standard antibiotics. Urine culture examination indicated Escherichia coli as the most predominant pathogen causing urinary tract infection. The minimum inhibitory concentration (MIC) value of cinnamon extract was found to be 0.452 μg whereas the MIC of erythromycin and tetracycline were 10 μg and 30 μg, respectively. The supplementation of cinnamon capsule did not have any effect on gram-negative bacteria.