Ponmurugan Karuppiah
@ksu.edu.sa
Research Associate, Department of Botany and Microbiology
King Saud University
RESEARCH INTERESTS
Actinomycetes,
Plant Growth Promoting Activities
Antimicrobials
Nanoparticles
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
V. Vahitha, Growther Lali, Saradh Prasad, Ponmurugan Karuppiah, Gopalu Karunakaran, and Mohamad S. AlSalhi
Springer Science and Business Media LLC
Vijayakumar Aishwarya, Chinnannan Karthik, Kannan Maharajan, Kadirvelu Krishna, Ponmurugan Karuppiah, Jothi Ramalingam Rajabathar, Govindaraju Ramkumar, and Ranganathan Keerthana
Elsevier BV
Abdulrahman I. Almansour, Natarajan Arumugam, Saied M. Soliman, E. Viswanathan, Necmi Dege, Ponmurugan Karuppiah, Dhanaraj Premnath, Jyothi Kumari, Dharmarajan Sriram, and Karthikeyan Perumal
Elsevier BV
Poomany Arul Soundara Rajan Yolin Angel, Palanisamy Jeyakumar, Arul Raj Jasmin Suriya, Aliyas Sheena, Ponmurugan Karuppiah, Govindasami Periyasami, Antony Stalin, and Kasi Murugan
Frontiers Media SA
IntroductionFungal keratitis (FK) poses a severe threat to vision, potentially leading to blindness if not promptly addressed. Clitoria ternatea flower extracts have a history of use in Ayurvedic and Indian traditional medicines, particularly for treating eye ailments. This study investigates the antifungal and antibiofilm effects of Clitoria ternatea flower extracts on the FK clinical isolate Coniochaeta hoffmannii. Structural details and key compound identification were analysed through FTIR and GC-MS.MethodsThe minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of Clitoria ternatea flower extracts were determined using broth dilution and well plate techniques. Biofilm inhibitory activity was assessed through microscopic evaluation, while anti-irritant and cytotoxic properties were evaluated using CAE-EI and MTT assays. Through GC-MS and FT-IR analysis the compounds dissolved in the extract and their functional group were studied, and their toxicity screening and pharmacokinetic prediction were conducted in silico. Subsequently, compounds with high corneal permeability were further identified, and molecular docking and simulation studies at 150 ns were used to investigate their interactions with fungal virulence factors and human inflammatory proteins.Results and DiscussionAt a concentration of 250 µg/mL, the Clitoria ternatea flower extract displayed effective biofilm inhibition. MIC and MFC values were determined as 500 and 1000 µg/mL, respectively. CAE-EI and MTT assays indicated no significant irritant and cytotoxic effects up to a concentration of 3 mg/mL. Compounds like 9,9-dimethoxybicyclo[3.3.1]nonane-2,4-dione showed high corneal permeability with strong and stable interactions with fungal virulence cellobiose dehydrogenase, endo β 1,4 xylanase, and glucanase, as well as corneal inflammation-associated human TNF-α and Interleukin IL-1b protein targets. The findings indicate that extracts from C. ternatea flowers could be formulated for an effective and safe alternative for developing new topical FK therapeutics.
Jasmin Suriya Arul Raj, Sheena Aliyas, Yolin Angel Poomany Arul Soundara Rajan, Kasi Murugan, Ponmurugan Karuppiah, Natarajan Arumugam, Abdulrahman I. Almansour, and Perumal Karthikeyan
Wiley
AbstractThe contemporary food industry's uses of nanoemulsions (NEs) include food processing, effective nutraceutical delivery, the development of functional chemicals, and the synthesis of natural preservatives, such as phytocompounds. Although cinnamon essential oil (CEO) is widely used in the cosmetic, pharmaceutical, and food industries, it is difficult to add to aqueous‐based food formulations due to its weak stability and poor water solubility. This study describes the formulation of a CEO nanoemulsion (CEONE) by spontaneous emulsification and evaluates its antibacterial and antibiofilm properties against biofilm‐forming Serratia rubidaea BFMO8 isolated from spoiled emperor fish (Lethrinus miniatus). Bacteria causing spoilage in emperor fish were isolated and identified as S. rubidaea using common morphological, cultural, and 16S RNA sequencing methods, and their ability to form biofilms and their susceptibility to CEONE were assessed using biofilm‐specific methods. The spontaneous emulsification formulation of CEONE was accomplished using water and Tween 20 surfactant by manipulating organic and aqueous phase interface properties and controlling particle growth by capping surfactant increases. The best emulsification, with highly stable nano‐size droplets, was accomplished at 750 rpm and a 1:3 ratio concentration. The stable CEONE droplet size, polydispersity index, and zeta potential values were 204.8 nm, 0.115, and −6.05 mV, respectively. FTIR and high‐resolution liquid chromatography‐mass spectrometry (HR‐LCMS) analyses have revealed carboxyl, carbonyl, and phenol‐like primary phytochemical functional groups in CEO and CEONE, which contribute to their antibacterial and antibiofilm properties.
Natarajan Arumugam, Khloud Ibrahim Al-Shemaimari, Mohammad Altaf, Karuppiah Ponmurugan, Dhanaraj Premnath, Sinouvassane Djearamane, Ling Shing Wong, and Saminathan Kayarohanam
Elsevier BV
Govindasami Periyasami, Ponmurugan Karuppiah, Perumal Karthikeyan, and Selvakumar Palaniappan
American Chemical Society (ACS)
Catheter-associated urinary tract infections (CAUTIs) frequently occur following the insertion of catheters in hospitalized patients, often leading to severe clinical complications. These complications are exacerbated by biofilm-forming organisms such as Staphylococcus aureus, contributing to the emergence of multidrug-resistant (MDR) strains, which complicates treatment strategies. This study aims to investigate the antibacterial, antibiofilm, and antiadhesive properties of duloxetine against S. aureus in the context of CAUTI. Our findings demonstrate that duloxetine exhibits significant antibacterial activity, as evidenced by the agar diffusion method. A minimal inhibitory concentration (MIC) of 37.5 μg/mL was established using the microdilution method. Notably, duloxetine displayed inhibitory effects against biofilm formation on polystyrene surfaces up to its MIC level, as demonstrated by the crystal violet method. Intriguingly, the study also revealed that duloxetine could prevent biofilm formation at lower concentrations and reduce mature biofilms, as confirmed by scanning electron microscopy (SEM) and quantitative biofilm assays. Furthermore, duloxetine-coated silicone catheter tubes exhibited antibacterial properties against S. aureus in a bladder model, visualized by confocal laser scanning microscopy (CLSM) and corroborated through FDA and PI staining, highlighting noticeable morphological changes in S. aureus post-treatment. In conclusion, this study presents duloxetine as a promising alternative agent with antibacterial and antiadhesive properties against S. aureus in the prevention and management of CAUTI, warranting further exploration in the clinical setting.
Abdulrahman I. Almansour, Manal Fahad Alkaltham, Natarajan Arumugam, Saied M. Soliman, Vedichi Madhu, Shatha Ibrahim Alaqeel, Necmi Dege, Ponmurugan Karuppiah, and Karthikeyan Perumal
Elsevier BV
Adhikesavan Harikrishnan, VijayKumar Veena, Roshini Kancharla, Sakshi Chavan, Jothi Ramalingam Rajabathar, Hamad Al-Lohedan, Saravanan Pandiaraj, Ponmurugan Karuppiah, and Selvaraj Arokiyaraj
Elsevier BV
Natarajan Arumugam, Saied M. Soliman, Vijayan Viswanathan, Abdulrahman I. Almansour, Raju Suresh Kumar, Sakkarapalayam M. Mahalingam, Bellie Sundaram Krishnamoorthy, Necmi Dege, Ponmurugan Karuppiah, and Karthikeyan Perumal
Elsevier BV
Nadarajan Prathap, Putrakumar Balla, Muthugoundar Subramanian Shivakumar, Govindasami Periyasami, Ponmurugan Karuppiah, Krishnaraj Ramasamy, and Srinivasan Venkatesan
Springer Science and Business Media LLC
AbstractCarbon dots have stimulated the curiosity of biomedical researchers due to their unique properties, such as less toxicity and high biocompatibility. The synthesis of carbon dots for biomedical application is a core area in research. In the current research, an eco-friendly hydrothermal technique was employed to synthesize high fluorescent, plant-derived carbon dots from Prosopis juliflora leaves extract (PJ-CDs). The synthesized PJ-CDs were investigated by physicochemical evaluation instruments such as fluorescence spectroscopy, SEM, HR-TEM, EDX, XRD, FTIR, and UV-Vis. The UV-Vis absorption peaks obtained at 270 nm due to carbonyl functional groups shifts of n→π*. In addition, a quantum yield of 7.88 % is achieved. The synthesized PJ-CDs showing the presence of carious functional groups O–H, C–H, C=O, O–H, C–N and the obtained particles in spherical shape with an average size of 8 nm. The fluorescence PJ-CDs showed stability against various environmental factors such as a broad range of ionic strength and pH gradient. The antimicrobial activity of PJ-CDs was tested against a Staphylococcus aureus, and a Escherichia coli. The results suggest that the PJ-CDs could substantially inhibit the growth of Staphylococcus aureus. The findings also indicate that PJ-CDs are effective materials for bio-imaging in Caenorhabditis elegans and they can be also used for pharmaceutical applications.
Thirumalaivasan Ramachandran, Devaprakash Manoharan, Sivakumar Natesan, Shyam Kumar Rajaram, Ponmurugan Karuppiah, Mohammed Rafi Shaik, Mujeeb Khan, and Baji Shaik
MDPI AG
Exopolysaccharides (EPS) are exogenous microbial metabolites generated predominantly during the development of bacteria. They have several biological potentials, including antibacterial, antioxidant, and anticancer actions. Polysaccharide-coated nanoparticles have high biological activity and are used in treatments and diagnostics. In this research, selenium nanoparticles (SeNPs) are synthesized and conjugated with bacterial (Bacillus sp. MKUST-01) exopolysaccharide (EPS). Initially, the creation of SeNPs conjugates was verified through UV–Vis spectral examination, which exhibited a prominent peak at 264 nm. Additionally, X-ray diffraction (XRD) analysis further substantiated the existence of crystalline Se, as evidenced by a robust reflection at 29.78°. Another reflection observed at 23.76° indicated the presence of carbon originating from the EPS. Fourier transform infrared spectroscopy (FT-IR) analysis of the EPS capped with SeNPs displayed characteristic peaks at 3425 cm−1, 2926 cm−1, 1639 cm−1, and 1411 cm−1, corresponding to the presence of O-H, C-H, C=O, and COO–groups. The SeNPs themselves were found to possess elongated rod-shaped structures with lengths ranging from 250 to 550 nm and a diameter of less than 70 nm, as confirmed using scanning electron microscopy and particle size analysis. In contrast to the SeNPs, the SeNPs–EPS conjugates showed no hemolytic activity. The overall antioxidant activity of SeNPs–EPS conjugates outperformed 20% higher than SeNPs and EPS. Additionally, experimental observations involving gnotobiotic Artemia nauplii experiments were also recorded, such as the supplementation of EPS and SeNPs–EPS conjugates corresponding to enhanced growth and increased survival rates compared to Artemia nauplii fed with SeNPs and a microalgal diet.
Vinnarasi A. Raj, Karthikumar Sankar, Pandiarajan Narayanasamy, Innasi Ganesh Moorthy, Natesan Sivakumar, Shyam Kumar Rajaram, Ponmurugan Karuppiah, Mohammed Rafi Shaik, Abdulrahman Alwarthan, Tae Hwan Oh,et al.
MDPI AG
Customer demand for natural packaging materials in the food industry has increased. Biocomposite films developed using boiled rice water could be an eco-friendly and cost-effective packaging product in the future. This study reports the development of bio-based films using waste materials, such as boiled rice water (matrix) and Pistacia vera shells (reinforcement material), using an adapted solution casting method. Several film combinations were developed using various concentrations of plasticizing agent (sorbitol), thickening agent (oil and agar), and stabilizing agents (Arabic gum, corn starch, and Pistacia vera shell powder). Various packaging properties of the film were analyzed and examined to select the best bio-based film for food packaging applications. The film fabricated with Pistacia vera shell powder in the biocomposite film exhibited a reduced water solubility, swelling index, and moisture content, as compared to polyethene packaging material, whereas the biocomposite film exhibited poor antimicrobial properties, high vapor transmission rate, and high biodegradability rate. The packaging properties and characterization of the film indicated that the boiled rice water film with Pistacia vera shell powder was suitable for packaging material applications.
Umamagesh Palaniappan, Jaianand Kannaiyan, Balaji Paulraj, Ponmurugan Karuppiah, Santhosh Basavarajappa, Asad Syed, Abdallah M. Elgorban, Nouf S. Zaghloul, and Veeramani Veeramanikandan
American Chemical Society (ACS)
Therapies based on mesenchymal stem cells have incredible potential for tissue regeneration. Tracking cells and keeping them at the injury site are creating challenges. The cells can be sown into a biocompatible scaffold as a possible remedy. Tissue engineering construction is a difficult, multistep process that requires many variables to be optimized, including the stem cell source, molecular components, scaffold architecture, and a suitable in vivo animal model. In order to locate a suitable regenerative scaffold for delivering stromal cells to regions with greater healing potential, we assessed whether human Wharton’s Jelly-derived mesenchymal stem cells (WJMSCs) responded on biological membranes. WJMSCs were isolated, characterized, and seeded onto an amniotic membrane-based scaffold. Results obtained in vitro revealed that the seeded scaffolds had a significant impact on a number of critical variables, including seeding effectiveness, cellular dispersion, adhesion, survival, and metabolic activity. The research sheds light on a fresh facet of material behavior and paves the way for the creation of scaffold materials that support tissue regeneration and repair. Furthermore, the methods used herein can be utilized to test other scaffold materials to increase their healing potential with WJMSCs.
Kalidoss Rajendran, Ponnusamy Ponmurugan, Balasubramanian Mythili Gnanamangai, Ponmurugan Karuppiah, Mohammed Rafi Shaik, Mujeeb Khan, Merajuddin Khan, and Baji Shaik
MDPI AG
Lichens are symbiotic organisms that are composed of fungal partners and photosynthetic algal partners. During the symbiotic process in lichen thallus, the fungus synthesizes certain secondary metabolites in which lecanoric acid is very important in terms of antibiotic properties. Considering the vital importance of lecanoric acid, the present study aimed to produce lecanoric acid from the thallus of Parmotrema austrosinense lichen using Modified Bold’s basal salt medium and evaluate the bio-efficacy against tea fungal pathogens. Lecanoric acid was purified and confirmed by micro-crystallization method and subsequently bioassayed against tea fungal pathogens. The results revealed that lecanoric acid registered a significant antifungal activity in terms of the growth inhibition of test pathogens. Companion systemic and botanical fungicides were found to be inferior to lecanoric acid in the percentage of growth inhibition. The inhibition rate varied among tea pathogens. Of the tea pathogens tested, tea leaf disease-causing pathogens including Cercospora theae (C. theae), Glomerella cingulata (G. cingulate), and Phomopsis theae (P. theae) showed the highest percentage of growth inhibition followed by stem and root rot diseases. The present study suggests that lecanoric acid showed an inhibitory effect against tea pathogens, which might be due to antibiotic properties and fungicidal action of lecanoric acid.
Selvaraju Sivamani, Prema Binnal, Capili Roy, Amal Al Khaldi, Fatema Al Hamar, J. Prakash Maran, N. Sivarajasekar, G. Rajeshkumar, Naif Abdullah Al-Dhabi, and Ponmurugan Karuppiah
Springer Science and Business Media LLC
Kalidoss Rajendran, Ponmurugan Karuppiah, Ponmurugan Ponnusamy, Mohammed Rafi Shaik, Mujeeb Khan, Tae Hwan Oh, and Baji Shaik
MDPI AG
Thousands of different kinds of lichen metabolites are being examined for their biological activities, including anticancer properties. In this context, the present study aims to assess the anti-inflammatory activity of the acetone extract of Parmotrema austrosinense mycobiont. A lipid peroxidation assay was performed with the acetone extracts of P. austrosinense mycobiont, which was further used to evaluate its anti-inflammatory efficacy using a zebrafish model. Furthermore, the histopathological study was also carried out with muscle tissues and amplification of its inflammation marker. The results revealed that the lichen compound (i.e., lecanoric acid) in the acetone extract of P. austrosinense possesses anti-inflammatory activity. Histopathology studies confirmed the decreased numbers of neutrophil cells in the 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced zebrafishes, as confirmed by changes in the fishes’ weight before and after the sample treatment, prompted by TNBS inflammation. The present results also demonstrated a dose-dependent decrease in the lipid peroxidation (LPO) levels in the muscle tissues of zebrafishes. Gene amplification studies suggested that the lichen compound might perform dose-dependent downregulation of the inflammatory gene marker of the tumor necrosis factor (TNF)-α gene; this further confirms that the extract should possess anti-inflammatory activity. As per the literature, this study is one of the most complete, comprehensive in vivo anti-inflammatory analyses in which inflammation was induced in zebrafish by using 2,4,6-trinitrobenzene sulfonic acid (TNBS). Particularly, this study successfully identified a bioactive compound isolated from the lichen P. austrosinense, and which exhibited decent anti-inflammatory activity.
Dharman Kalaimurgan, Kandhasamy Lalitha, Rasiravathanahalli Kaveriyappan Govindarajan, Kridsada Unban, Muthugoundar Subramanian Shivakumar, Srinivasan Venkatesan, Chartchai Khanongnuch, Fohad Mabood Husain, Faizan Abul Qais, Imran Hasan,et al.
Springer Science and Business Media LLC
Kiruthiga Periyannan, Hemamala Selvaraj, Balachandar Subbu, Muthukrishnan Pallikondaperumal, Ponmurugan Karuppiah, Jothi Ramalingam Rajabathar, Hamad Al-Lohedan, and Sadhasivam Thangarasu
Walter de Gruyter GmbH
Abstract The exoskeletons of crabs, shrimp, and fish are major waste. These wastes contain chitin, an abundant natural polymer found next to cellulose. Thus, disposal of this waste becomes a huge problem for the environment; besides this, reutilization boosts the circular economy. Chitin is partially deacetylated to yield the economically useful product of chitosan and is a heteropolymer. The current study isolated chitosan from mushrooms and various marine crustaceans, i.e., crabs, shrimp, and fish. Chitosan was extracted from marine crustaceans by demineralization, deproteination, and deacetylation. Later, extracted chitosan was characterized by physicochemical characteristics like deacetylation degree, ash content, protein, color, fat-binding capacity (FBC), water-binding capacity (WBC), pH, and moisture content. The result showed that chitosan yield ranges from 13.0% to 17.0%, the degree of deacetylation range from 82.0% to 85.0%, ash content range from 0.8% to 3.0%, and protein content is below 1.0%. The FBC and WBC range between 320% and 444% and 535% and 602%, respectively. The pH and moisture content range from 7.4 to 8.0 and from 2.0% to 4.0%, respectively. Overall, results specified that crustacean waste was an exceptional chitosan source with availability and production consistency.
Uma Eswaranpillai, Priyadharsini Murugesan, and Ponmurugan Karuppiah
Horizon E-Publishing Group
Many people suffer from a deficiency of essential micronutrients. Sprouts and microgreens can transform the whole idea of vegetables to resolve the need for a diet with fresh, nutrient-rich, and high content of phyto-compounds necessary for a healthy body. The study's main objective is to evaluate the growth of 6 different seeds, such as four legumes; fenugreek, mung bean, cowpea, horse gram and two grains, wheat, sorghum microgreens. All the seeds were cultivated in soil, water and coco peat, to estimate and compare the nutritional properties of the selected sprouts vs. microgreens. The growth of microgreens in each medium was evaluated, and the proximate and nutritional properties were analysed. In terms of the growth of microgreens, coco peat medium serves the best, as it retains water for a long and it is porous to provide better aeration for the roots and also the day of harvest is shorter. In terms of the nutritional property of microgreens, soil serves the best, as it contains more nutrients than any other medium. The study results showed sprouts are better sources of proteins and carbohydrates than microgreens. However, microgreens were characterized by a high content of carotenoids, chlorophylls and ascorbic acid. It also exhibiting higher anti-diabetic and anticholinergic activity than sprouts. In addition, the microgreens have more micronutrients like zinc, copper, iron, magnesium, potassium etc., than the sprouts. Finally, microgreens were better growing with coco peat and also sources for functional components for dietary supplements and sustainable agriculture.
Dharman Kalaimurgan, Kandhasamy Lalitha, Rasiravathanahalli Kaveriyappan Govindarajan, Kridsada Unban, Muthugoundar Subramanian Shivakumar, Srinivasan Venkatesan, Chartchai Khanongnuch, Fohad Mabood Husain, Faizan Abul Qais, Imran Hasan,et al.
Springer Science and Business Media LLC
Uma Eswaranpillai, Yhasasuwini Senthilramesh, and Ponmurugan Karuppiah
ANSF Publications
Diet and nutrition are important factors in promoting and maintaining good health. Fruits are important sources of protective foods that are highly beneficial for maintaining good health and preventing diseases.The present study aimed to assess the nutritional profiling (carbohydrates, proteins, lipids, moisture, ash, solids and vitamins content), anti-nutritional (oxalate, saponin and tannin) and physico-chemical (shape, colour, size and weight) parameters of Chrysophyllum oliviforme fruits. The nutritional contents were determined by Biuret, dry ash, and oven dry method for protein, ash and moisture content, respectively. The nutritional values of this fruit were: carbohydrates (5.30±0.21 mg/g), protein (6.30±0.19), lipids (14.00 %), thiamine (0.04 mg/g), calcium (3.29 mg/kg), potassium (348-370 mg/100 g). The results revealed that this plant C. oliviforme has a high nutritious value and suggested that its underutilized edible fruits could be a source of protein, vitamins, and minerals.The study on underutilized edible fruit plant C.oliviforme L.appears to be the first report on its nutritional evaluation.This data may also helpimprove the nutritional and health status of rural populations nationwide by utilizing underutilized and neglected specialities.
Lavanya Kubendiran, Sowmiya Theerthagiri, Naif Abdullah Al-Dhabi, Senthilkumar Palaninaicker, Shivakumar Muthugoundar Subramanian, Venkatesan Srinivasan, and Ponmurugan Karuppiah
Springer Science and Business Media LLC
Ointments are widely used as topical medication as a mode of transdermal drug delivery for treatment of skin lesions. In this study, Tridax procumbens infused oil (oil phase) and Gelatin stabilized silver nanoparticle (Aqueous phase) were blended with natural biosurfactant extracted from Lactobacillus casei (MT012285) to prepare an ointment for topical wounds. GC–MS of infused oil showed the presence of pentadecanoic acid and 1,19-eicosadiene. FT-IR studies show the presence of aliphatic and amino group of biosurfactant from L. casei confirm the presence of lipopeptide, which can form stable W/O emulsion in ointment. The prepared ointment shows higher microbial reduction capacity on clinical pathogens tested namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Klebsiella pneumoniae. Further, herbal-nano-ointment exhibits minimal hemolytic action on human red blood cells at 100 µg/mL. At higher concentration of 4.0 mg/mL of ointment low cytotoxic effect on L929 fibroblastic cell line was observed. Cell migration rate of 62% was recorded at 24 h using wound scratch assay. Based on the study we find that biosurfactant-based nano-topical ointment from T. procumbens oil with G-AgNPs, has low cytotoxic effect and a high cell migration rate and hence can be used in the topical application for treatment of wound.
Sri Shaila Purna Kanagaraj, Shyam Kumar Rajaram, Maqusood Ahamed, Shajahan Subedhar, Karthikumar Sankar, Ganesh Moorthy Innasimuthu, and Ponmurugan Karuppiah
Springer Science and Business Media LLC
In the recent past, the nanobiotechnology field has grown up tremendously with more applications because the synthesis process will be more practically possible techniques. Since, plant-derived biosynthesis of silver nanoparticles is superior since its eco-friendly, cost-efficient, speedy process in nature. Alginate-based eco-friendly, biodegradable nanocomposite films were prepared with Celosia cristata leaf extracts silver nanoparticles (CC-AgNPs) and sodium alginate by simple casting techniques. The synthesized alginate–silver (Alg-Ag) nanocomposite films were characterized and confirmed by UV/Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), thermogravimetric analysis (TGA) and atomic force microscopy (AFM). The various characterization process was further confirmed synthesized CC-AgNPs and Alg-Ag nanocomposite films. In addition, TEM and SEM monogram also showed uniformity and smooth surface of Alg-Ag nanocomposite films. The nanocomposite films displayed effective antimicrobial proficiency against selected foodborne pathogens i.e., Staphylococcus aureus, Salmonella typhimurium and Clostridium perfringens. Further, the Alg-Ag nanocomposite films resolve and eliminate the microbial intruders and enhance food stuff's quality and storage stability.